pycom03g21460

CSC1-like protein At4g02900

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0001682
Physical Location & Seq
Forward (+)
21756568 .. 21757802
1235 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g21460.4

Sequence Viewer

Length: 936 bp
ATGACTGGTTTCTTGGGCCTGTGGGGTGGAAAGATCGGGAAGTTGAGCGAAGAAGTGAGTATTTCTAGTATTCACAAAAACTGTTGGAGGTCCATCCCAATGAAAGCCACATTCTTCATCACTTATATCATGGTTGATGATTGGAGTGGAATCGCTGCAGAAATCCTCAGATTGACGCCGTTTATTTTGTTTCACCTCAAGAACGCATTTCTGGTTACGACAAAACAAGATCGGGAGCAGGCAATTGATCTCGGTTCCTTGAATTTCGCTACAAACGAACCCAGGACACAGTTATATTTTCTACTCGGGCTAGTGTATTCTGTTGTCACGCCATTACTTCTTCCTTTCATTCTCATCTTCTTCGCTTTCGCCTTCCTAGTTTATTGTCATCTGCTCATTGTTACGGTGGCACTAATGGTTCCTTTTTGCTGCCAGATTATTAATGTCTATGACCAGAAATATGAGAGTGGTGCAGCCTTCTGGCCACAAGTTCATCTTCGCGTAATCATATGTCTAATCATAGCACAAGTTCTACTAATGCGGCTGTTCGGCACATTAGGCGTTGCAAAATCAACGTTCATTCTCATCGTGCAACCTATTTTGACAATCTGGCTCCATAGAGTCTGCAAGGGACGTTTTGGAATCAGCATTCGTAAAATTCCCACTACAGCATTACCATTTTCTCAATGTGCTTGGTTTGGCGAAAAACAGGAGGCAATGATGAAGGATACGGTCGAGCGCGCAACGGAACCAAACCTGAACTTGATGACCTATCTGAAGGACGCTTACGTGCACCCCGTCTTCAAGGGAGGGCAGATGCAGAAGCAGGAAGTAGAAGTTGATGAAGAAGGGAGCAGTCCTCTTGTTCGTACAAAGAGAACGTCTCAAATGGGTAGTAAACACGAATCTGATGCCAGTCCGCAAATTCGCAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PHM7_cyt RSN1_7TM RSN1_TM

Protein Analysis

312

Amino Acids

35.25

Weight (kDa)

8.91

Isoelectric Point (pI)

35.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 501, 741
AciI CCGC 2 cut(s) 541, 920
AclI AACGTT 1 cut(s) 575
AcoI YGGCCR 1 cut(s) 482
AcsI RAATTY 3 cut(s) 262, 657, 924
AcuI CTGAAG 1 cut(s) 797
AcyI GRCGYC 1 cut(s) 176
AfaI GTAC 1 cut(s) 871
AgsI TTSAA 2 cut(s) 262, 805
AjnI CCWGG 1 cut(s) 281
Alw21I GWGCWC 1 cut(s) 795
Alw26I GTCTC 1 cut(s) 888
Alw44I GTGCAC 1 cut(s) 791
Ama87I CYCGRG 1 cut(s) 305
AoxI GGCC 2 cut(s) 16, 482
ApaLI GTGCAC 1 cut(s) 791
ApeKI GCWGC 3 cut(s) 155, 429, 473
ApoI RAATTY 3 cut(s) 262, 657, 924
ArsI GACNNNNNNTTYG 2 cut(s) 866, 898
AseI ATTAAT 1 cut(s) 441
AspLEI GCGC 2 cut(s) 741, 743
AspS9I GGNCC 2 cut(s) 16, 90
AsuHPI GGTGA 1 cut(s) 185
AvaI CYCGRG 1 cut(s) 305
AvaII GGWCC 1 cut(s) 90
BaeGI GKGCMC 1 cut(s) 795
BalI TGGCCA 1 cut(s) 484
BarI GAAGNNNNNNTAC 2 cut(s) 770, 802
BbsI GAAGAC 1 cut(s) 793
Bbv12I GWGCWC 1 cut(s) 795
BbvI GCAGC 3 cut(s) 142, 416, 485
BccI CCATC 1 cut(s) 101
BceAI ACGGC 1 cut(s) 163
BcgI CGANNNNNNTGC 2 cut(s) 893, 927
BciT130I CCWGG 1 cut(s) 283
BciVI GTATCC 1 cut(s) 721
BcoDI GTCTC 1 cut(s) 888
BfaI CTAG 3 cut(s) 66, 311, 377
BfmI CTRYAG 2 cut(s) 156, 666
BfuI GTATCC 1 cut(s) 721
BisI GCNGC 4 cut(s) 156, 430, 474, 542
BlsI GCNGC 4 cut(s) 157, 431, 475, 543
Bme1390I CCNGG 1 cut(s) 283
Bme18I GGWCC 1 cut(s) 90
BmeT110I CYCGRG 1 cut(s) 305
BmgT120I GGNCC 2 cut(s) 16, 90
BmiI GGNNCC 4 cut(s) 256, 420, 614, 750
BmrFI CCNGG 1 cut(s) 283
BmsI GCATC 2 cut(s) 807, 901
BpiI GAAGAC 1 cut(s) 793
BplI GAGNNNNNCTC 4 cut(s) 844, 876, 868, 900
BpuEI CTTGAG 1 cut(s) 182
BsaAI YACGTR 1 cut(s) 790
BsaHI GRCGYC 1 cut(s) 176
BsaJI CCNNGG 1 cut(s) 281
Bse1I ACTGG 2 cut(s) 10, 915
Bse3DI GCAATG 1 cut(s) 723
BseBI CCWGG 1 cut(s) 283
BseDI CCNNGG 1 cut(s) 281
BseGI GGATG 1 cut(s) 93
BseMI GCAATG 1 cut(s) 723
BseMII CTCAG 1 cut(s) 181
BseNI ACTGG 2 cut(s) 10, 915
BsePI GCGCGC 1 cut(s) 739
BseSI GKGCMC 1 cut(s) 795
BseXI GCAGC 3 cut(s) 142, 416, 485
BsgI GTGCAG 1 cut(s) 492
Bsh1236I CGCG 2 cut(s) 501, 741
Bsh1285I CGRYCG 1 cut(s) 735
BshFI GGCC 2 cut(s) 18, 484
BsiEI CGRYCG 1 cut(s) 735
BsiHKAI GWGCWC 1 cut(s) 795
BsiHKCI CYCGRG 1 cut(s) 305
BslFI GGGAC 1 cut(s) 645
BsmAI GTCTC 1 cut(s) 888
BsmBI CGTCTC 1 cut(s) 888
BsmFI GGGAC 1 cut(s) 645
BsmI GAATGC 1 cut(s) 648
BsnI GGCC 2 cut(s) 18, 484
BsoBI CYCGRG 1 cut(s) 305
Bsp1286I GDGCHC 1 cut(s) 795
Bsp143I GATC 3 cut(s) 33, 229, 247
BspACI CCGC 2 cut(s) 541, 920
BspANI GGCC 2 cut(s) 18, 484
BspCNI CTCAG 1 cut(s) 180
BspFNI CGCG 2 cut(s) 501, 741
BspLI GGNNCC 4 cut(s) 256, 420, 614, 750
BspMAI CTGCAG 1 cut(s) 160
BsrDI GCAATG 1 cut(s) 723
BsrI ACTGG 2 cut(s) 10, 915
BssECI CCNNGG 1 cut(s) 281
BssHII GCGCGC 1 cut(s) 739
BssMI GATC 3 cut(s) 33, 229, 247
BssNI GRCGYC 1 cut(s) 176
Bst2UI CCWGG 1 cut(s) 283
Bst4CI ACNGT 4 cut(s) 83, 291, 406, 733
BstACI GRCGYC 1 cut(s) 176
BstBAI YACGTR 1 cut(s) 790
BstC8I GCNNGC 2 cut(s) 240, 741
BstDEI CTNAG 1 cut(s) 167
BstF5I GGATG 1 cut(s) 93
BstFNI CGCG 2 cut(s) 501, 741
BstHHI GCGC 2 cut(s) 741, 743
BstKTI GATC 3 cut(s) 36, 232, 250
BstMAI GTCTC 1 cut(s) 888
BstMBI GATC 3 cut(s) 33, 229, 247
BstMCI CGRYCG 1 cut(s) 735
BstMWI GCNNNNNNNGC 1 cut(s) 558
BstNI CCWGG 1 cut(s) 283
BstSCI CCNGG 1 cut(s) 281
BstSFI CTRYAG 2 cut(s) 156, 666
BstSLI GKGCMC 1 cut(s) 795
BstUI CGCG 2 cut(s) 501, 741
BstV1I GCAGC 3 cut(s) 142, 416, 485
BstV2I GAAGAC 1 cut(s) 793
BsuI GTATCC 1 cut(s) 721
BsuRI GGCC 2 cut(s) 18, 484
BtsCI GGATG 1 cut(s) 93
Cac8I GCNNGC 2 cut(s) 240, 741
CfoI GCGC 2 cut(s) 741, 743
Cfr13I GGNCC 2 cut(s) 16, 90
CseI GACGC 2 cut(s) 184, 791
Csp6I GTAC 1 cut(s) 870
CviAII CATG 1 cut(s) 130
CviJI RGCY 7 cut(s) 18, 107, 310, 476, 484, 544, 613
CviKI_1 RGCY 7 cut(s) 18, 107, 310, 476, 484, 544, 613
CviQI GTAC 1 cut(s) 870
DdeI CTNAG 1 cut(s) 167
DpnI GATC 3 cut(s) 35, 231, 249
DpnII GATC 3 cut(s) 33, 229, 247
EaeI YGGCCR 1 cut(s) 482
Eco47I GGWCC 1 cut(s) 90
Eco57I CTGAAG 1 cut(s) 797
Eco88I CYCGRG 1 cut(s) 305
EcoRII CCWGG 1 cut(s) 281
Esp3I CGTCTC 1 cut(s) 888
FaeI CATG 1 cut(s) 133
FaiI YATR 9 cut(s) 126, 131, 295, 450, 462, 509, 511, 521, 618
FalI AAGNNNNNCTT 2 cut(s) 480, 512
FaqI GGGAC 1 cut(s) 645
FatI CATG 1 cut(s) 129
FauNDI CATATG 1 cut(s) 509
Fnu4HI GCNGC 4 cut(s) 156, 430, 474, 542
FokI GGATG 1 cut(s) 80
Fsp4HI GCNGC 4 cut(s) 156, 430, 474, 542
FspBI CTAG 3 cut(s) 66, 311, 377
GlaI GCGC 2 cut(s) 740, 742
GluI GCNGC 4 cut(s) 156, 430, 474, 542
HaeIII GGCC 2 cut(s) 18, 484
HgaI GACGC 2 cut(s) 184, 791
HhaI GCGC 2 cut(s) 741, 743
Hin1I GRCGYC 1 cut(s) 176
Hin1II CATG 1 cut(s) 133
Hin6I GCGC 2 cut(s) 739, 741
HinP1I GCGC 2 cut(s) 739, 741
HinfI GANTC 4 cut(s) 150, 621, 642, 905
HphI GGTGA 1 cut(s) 185
Hpy166II GTNNAC 2 cut(s) 793, 899
Hpy188I TCNGA 3 cut(s) 170, 777, 910
Hpy188III TCNNGA 3 cut(s) 37, 199, 233
Hpy8I GTNNAC 2 cut(s) 793, 899
HpyAV CCTTC 5 cut(s) 382, 487, 718, 772, 842
HpyCH4III ACNGT 4 cut(s) 83, 291, 406, 733
HpyCH4IV ACGT 4 cut(s) 575, 634, 789, 881
HpyCH4V TGCA 7 cut(s) 158, 473, 566, 592, 627, 793, 820
HpyF10VI GCNNNNNNNGC 1 cut(s) 558
HpyF3I CTNAG 1 cut(s) 167
HpySE526I ACGT 4 cut(s) 575, 634, 789, 881
Hsp92I GRCGYC 1 cut(s) 176
Hsp92II CATG 1 cut(s) 133
HspAI GCGC 2 cut(s) 739, 741
Kzo9I GATC 3 cut(s) 33, 229, 247
LmnI GCTCC 3 cut(s) 235, 618, 852
Lsp1109I GCAGC 3 cut(s) 142, 416, 485
LweI GCATC 2 cut(s) 807, 901
MaeI CTAG 3 cut(s) 66, 311, 377
MaeII ACGT 4 cut(s) 575, 634, 789, 881
MaeIII GTNAC 3 cut(s) 214, 325, 400
MalI GATC 3 cut(s) 35, 231, 249
MboI GATC 3 cut(s) 33, 229, 247
MboII GAAGA 8 cut(s) 62, 106, 332, 349, 352, 488, 793, 857
MfeI CAATTG 1 cut(s) 243
MhlI GDGCHC 1 cut(s) 795
MlsI TGGCCA 1 cut(s) 484
MluCI AATT 4 cut(s) 243, 262, 657, 924
MluNI TGGCCA 1 cut(s) 484
MlyI GAGTC 1 cut(s) 630
MmeI TCCRAC 1 cut(s) 65
MnlI CCTC 6 cut(s) 81, 176, 206, 706, 803, 870
Mox20I TGGCCA 1 cut(s) 484
MscI TGGCCA 1 cut(s) 484
MseI TTAA 1 cut(s) 441
MslI CAYNNNNRTG 1 cut(s) 98
Msp20I TGGCCA 1 cut(s) 484
MspR9I CCNGG 1 cut(s) 283
MunI CAATTG 1 cut(s) 243
Mva1269I GAATGC 1 cut(s) 648
MvaI CCWGG 1 cut(s) 283
MvnI CGCG 2 cut(s) 501, 741
MwoI GCNNNNNNNGC 1 cut(s) 558
NdeI CATATG 1 cut(s) 509
NdeII GATC 3 cut(s) 33, 229, 247
NlaIII CATG 1 cut(s) 133
NlaIV GGNNCC 4 cut(s) 256, 420, 614, 750
NmuCI GTSAC 1 cut(s) 325
PauI GCGCGC 1 cut(s) 739
PcsI WCGNNNNNNNCGW 2 cut(s) 273, 795
PctI GAATGC 1 cut(s) 648
PfeI GAWTC 3 cut(s) 150, 642, 905
PkrI GCNGC 4 cut(s) 157, 431, 475, 543
PleI GAGTC 1 cut(s) 629
PpsI GAGTC 1 cut(s) 629
Ppu21I YACGTR 1 cut(s) 790
PshBI ATTAAT 1 cut(s) 441
Psp1406I AACGTT 1 cut(s) 575
Psp6I CCWGG 1 cut(s) 281
PspGI CCWGG 1 cut(s) 281
PspN4I GGNNCC 4 cut(s) 256, 420, 614, 750
PspPI GGNCC 2 cut(s) 16, 90
PstI CTGCAG 1 cut(s) 160
PteI GCGCGC 1 cut(s) 739
RsaI GTAC 1 cut(s) 871
RsaNI GTAC 1 cut(s) 870
RseI CAYNNNNRTG 1 cut(s) 98
SaqAI TTAA 1 cut(s) 441
SatI GCNGC 4 cut(s) 156, 430, 474, 542
Sau3AI GATC 3 cut(s) 33, 229, 247
Sau96I GGNCC 2 cut(s) 16, 90
SchI GAGTC 1 cut(s) 630
ScrFI CCNGG 1 cut(s) 283
SduI GDGCHC 1 cut(s) 795
SetI ASST 9 cut(s) 92, 198, 578, 598, 637, 759, 773, 792, 884
SfaNI GCATC 2 cut(s) 807, 901
SfcI CTRYAG 2 cut(s) 156, 666
SinI GGWCC 1 cut(s) 90
SmiMI CAYNNNNRTG 1 cut(s) 98
SmlI CTYRAG 1 cut(s) 197
SmoI CTYRAG 1 cut(s) 197
Sse9I AATT 4 cut(s) 243, 262, 657, 924
SsiI CCGC 2 cut(s) 541, 920
SspMI CTAG 3 cut(s) 66, 311, 377
StyD4I CCNGG 1 cut(s) 281
TaaI ACNGT 4 cut(s) 83, 291, 406, 733
TaiI ACGT 4 cut(s) 578, 637, 792, 884
TaqI TCGA 1 cut(s) 735
TasI AATT 4 cut(s) 243, 262, 657, 924
TauI GCSGC 1 cut(s) 544
TfiI GAWTC 3 cut(s) 150, 642, 905
Tru1I TTAA 1 cut(s) 441
Tru9I TTAA 1 cut(s) 441
TseFI GTSAC 1 cut(s) 325
TseI GCWGC 3 cut(s) 155, 429, 473
Tsp45I GTSAC 1 cut(s) 325
TspDTI ATGAA 7 cut(s) 106, 116, 337, 482, 568, 737, 858
TspGWI ACGGA 1 cut(s) 761
VneI GTGCAC 1 cut(s) 791
VpaK11BI GGWCC 1 cut(s) 90
VspI ATTAAT 1 cut(s) 441
XapI RAATTY 3 cut(s) 262, 657, 924
XspI CTAG 3 cut(s) 66, 311, 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.