pycom04g00010

Protein of unknown function (DUF642)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0002157
Physical Location & Seq
Reverse (-)
8431 .. 8700
270 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g00010.1

Sequence Viewer

Length: 270 bp
ATGGTCAATTTTGCCCACAAAGCCGGCGGGTGCCTAAAACTTCCCGACGTCAATTCGTCGTCTACAGTGGTCCAACGGGGTCAAGGTTGGTTGGGTTTTGCTCCTGGGGGATGGGCTACAAAGCCCAAGTGGTGGCATCGGCCATCGCTTTGCCGATTTGCCGGAAAATCGAAAAGGGTGGCCGGAGCACCATTGATTTTCGTCAACTTTCGTGGCCTCAAACCGCCACATACCATGGTTAATCAAGGTGTTCTTGGGTGGGTTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF642

Protein Analysis

90

Amino Acids

9.71

Weight (kDa)

11.51

Isoelectric Point (pI)

28.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 51
AccB1I GGYRCC 1 cut(s) 30
AccB7I CCANNNNNTGG 1 cut(s) 132
AccI GTMKAC 1 cut(s) 62
AciI CCGC 2 cut(s) 27, 224
AcoI YGGCCR 2 cut(s) 140, 180
AcyI GRCGYC 1 cut(s) 48
AfiI CCNNNNNNNGG 1 cut(s) 132
AjnI CCWGG 1 cut(s) 103
Alw21I GWGCWC 1 cut(s) 190
AoxI GGCC 3 cut(s) 140, 180, 214
AspS9I GGNCC 1 cut(s) 70
AvaII GGWCC 1 cut(s) 70
BanI GGYRCC 1 cut(s) 30
Bbv12I GWGCWC 1 cut(s) 190
BccI CCATC 2 cut(s) 105, 151
BciT130I CCWGG 1 cut(s) 105
BfmI CTRYAG 1 cut(s) 63
Bme1390I CCNGG 1 cut(s) 105
Bme18I GGWCC 1 cut(s) 70
BmgT120I GGNCC 1 cut(s) 70
BmiI GGNNCC 1 cut(s) 32
BmrFI CCNGG 1 cut(s) 105
BmsI GCATC 1 cut(s) 145
BsaHI GRCGYC 1 cut(s) 48
BsaJI CCNNGG 2 cut(s) 104, 234
Bsc4I CCNNNNNNNGG 1 cut(s) 132
Bse118I RCCGGY 1 cut(s) 23
BseBI CCWGG 1 cut(s) 105
BseDI CCNNGG 2 cut(s) 104, 234
BseGI GGATG 1 cut(s) 116
BseLI CCNNNNNNNGG 1 cut(s) 132
BshFI GGCC 3 cut(s) 142, 182, 216
BshNI GGYRCC 1 cut(s) 30
BsiHKAI GWGCWC 1 cut(s) 190
BsiSI CCGG 3 cut(s) 24, 162, 183
BslI CCNNNNNNNGG 1 cut(s) 132
BsnI GGCC 3 cut(s) 142, 182, 216
Bsp1286I GDGCHC 1 cut(s) 190
Bsp19I CCATGG 1 cut(s) 234
BspACI CCGC 2 cut(s) 27, 224
BspANI GGCC 3 cut(s) 142, 182, 216
BspLI GGNNCC 1 cut(s) 32
BspT107I GGYRCC 1 cut(s) 30
BsrFI RCCGGY 1 cut(s) 23
BssAI RCCGGY 1 cut(s) 23
BssECI CCNNGG 2 cut(s) 104, 234
BssNI GRCGYC 1 cut(s) 48
BssT1I CCWWGG 1 cut(s) 234
Bst2UI CCWGG 1 cut(s) 105
Bst4CI ACNGT 1 cut(s) 67
BstACI GRCGYC 1 cut(s) 48
BstC8I GCNNGC 1 cut(s) 25
BstDSI CCRYGG 1 cut(s) 234
BstF5I GGATG 1 cut(s) 116
BstMWI GCNNNNNNNGC 1 cut(s) 20
BstNI CCWGG 1 cut(s) 105
BstSCI CCNGG 1 cut(s) 103
BstSFI CTRYAG 1 cut(s) 63
BsuRI GGCC 3 cut(s) 142, 182, 216
BtgI CCRYGG 1 cut(s) 234
BtgZI GCGATG 1 cut(s) 129
BtsCI GGATG 1 cut(s) 116
BtsIMutI CAGTG 1 cut(s) 72
Cac8I GCNNGC 1 cut(s) 25
Cfr10I RCCGGY 1 cut(s) 23
Cfr13I GGNCC 1 cut(s) 70
CspCI CAANNNNNGTGG 2 cut(s) 193, 228
CviAII CATG 1 cut(s) 235
CviJI RGCY 6 cut(s) 23, 116, 124, 142, 182, 216
CviKI_1 RGCY 6 cut(s) 23, 116, 124, 142, 182, 216
EaeI YGGCCR 2 cut(s) 140, 180
Eco130I CCWWGG 1 cut(s) 234
Eco47I GGWCC 1 cut(s) 70
EcoRII CCWGG 1 cut(s) 103
EcoT14I CCWWGG 1 cut(s) 234
ErhI CCWWGG 1 cut(s) 234
FaeI CATG 1 cut(s) 238
FaiI YATR 2 cut(s) 231, 236
FalI AAGNNNNNCTT 2 cut(s) 237, 269
FatI CATG 1 cut(s) 234
FauI CCCGC 1 cut(s) 20
FblI GTMKAC 1 cut(s) 62
FokI GGATG 1 cut(s) 123
HaeIII GGCC 3 cut(s) 142, 182, 216
HapII CCGG 3 cut(s) 24, 162, 183
Hin1I GRCGYC 1 cut(s) 48
Hin1II CATG 1 cut(s) 238
HincII GTYRAC 1 cut(s) 205
HindII GTYRAC 1 cut(s) 205
HpaII CCGG 3 cut(s) 24, 162, 183
Hpy166II GTNNAC 2 cut(s) 63, 205
Hpy188III TCNNGA 1 cut(s) 44
Hpy8I GTNNAC 2 cut(s) 63, 205
Hpy99I CGWCG 2 cut(s) 50, 61
HpyCH4III ACNGT 1 cut(s) 67
HpyCH4IV ACGT 1 cut(s) 48
HpyF10VI GCNNNNNNNGC 1 cut(s) 20
HpySE526I ACGT 1 cut(s) 48
Hsp92I GRCGYC 1 cut(s) 48
Hsp92II CATG 1 cut(s) 238
KroI GCCGGC 1 cut(s) 23
KroNI GCCGGC 1 cut(s) 25
LmnI GCTCC 2 cut(s) 106, 185
LpnPI CCDG 5 cut(s) 37, 90, 117, 175, 196
LweI GCATC 1 cut(s) 145
MaeII ACGT 1 cut(s) 48
MhlI GDGCHC 1 cut(s) 190
MluCI AATT 2 cut(s) 7, 52
MmeI TCCRAC 1 cut(s) 97
MnlI CCTC 1 cut(s) 227
MroNI GCCGGC 1 cut(s) 23
MseI TTAA 1 cut(s) 240
MspI CCGG 3 cut(s) 24, 162, 183
MspR9I CCNGG 1 cut(s) 105
MvaI CCWGG 1 cut(s) 105
MwoI GCNNNNNNNGC 1 cut(s) 20
NaeI GCCGGC 1 cut(s) 25
NcoI CCATGG 1 cut(s) 234
NgoMIV GCCGGC 1 cut(s) 23
NlaIII CATG 1 cut(s) 238
NlaIV GGNNCC 1 cut(s) 32
PdiI GCCGGC 1 cut(s) 25
PflMI CCANNNNNTGG 1 cut(s) 132
Psp6I CCWGG 1 cut(s) 103
PspGI CCWGG 1 cut(s) 103
PspN4I GGNNCC 1 cut(s) 32
PspPI GGNCC 1 cut(s) 70
SaqAI TTAA 1 cut(s) 240
Sau96I GGNCC 1 cut(s) 70
ScrFI CCNGG 1 cut(s) 105
SduI GDGCHC 1 cut(s) 190
SetI ASST 3 cut(s) 51, 88, 250
SfaNI GCATC 1 cut(s) 145
SfcI CTRYAG 1 cut(s) 63
SinI GGWCC 1 cut(s) 70
Sse9I AATT 2 cut(s) 7, 52
SsiI CCGC 2 cut(s) 27, 224
StyD4I CCNGG 1 cut(s) 103
StyI CCWWGG 1 cut(s) 234
TaaI ACNGT 1 cut(s) 67
TaiI ACGT 1 cut(s) 51
TaqI TCGA 1 cut(s) 170
TasI AATT 2 cut(s) 7, 52
Tru1I TTAA 1 cut(s) 240
Tru9I TTAA 1 cut(s) 240
TscAI CASTG 1 cut(s) 72
TspRI CASTG 1 cut(s) 72
Van91I CCANNNNNTGG 1 cut(s) 132
VpaK11BI GGWCC 1 cut(s) 70
XmiI GTMKAC 1 cut(s) 62
ZraI GACGTC 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.