pycom09g08170

Protein of unknown function (DUF642)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0002157
Physical Location & Seq
Reverse (-)
6267412 .. 6267717
306 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g08170.1

Sequence Viewer

Length: 306 bp
ATGAAGATCTCAGTGAGTGGAGCAACTTTTATACGTACCACGAAGTCCAAAAGTGGACGAAAGATGGTCAATTTTGCCCACAAAGCCGGCGGGTGCCTAAAACTTCCCGATGTCGATTCGTCTTCTACAGTGGTCCAACCGGGTCAAGGTTGGTTGGGTTTTTCTCCTGGGATGATGGGCTACAAAGCCCAAGTGGTGGCATCGGCCATCACTTTGCCGATTTTCCGGAAAATCAAAAAGAGTGGTCGGAGCACCGTTGATTTTTGTCGACTTTCGTGGCCTCAAACCGCCACATACCATGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF642

Protein Analysis

102

Amino Acids

10.88

Weight (kDa)

10.46

Isoelectric Point (pI)

30.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 93
AccB7I CCANNNNNTGG 1 cut(s) 196
AccI GTMKAC 1 cut(s) 268
AccIII TCCGGA 1 cut(s) 225
AciI CCGC 2 cut(s) 90, 288
AcoI YGGCCR 1 cut(s) 204
AfaI GTAC 1 cut(s) 37
AfiI CCNNNNNNNGG 2 cut(s) 146, 196
AjnI CCWGG 1 cut(s) 166
Alw21I GWGCWC 1 cut(s) 254
Aor13HI TCCGGA 1 cut(s) 225
AoxI GGCC 2 cut(s) 204, 278
AspS9I GGNCC 1 cut(s) 133
AsuC2I CCSGG 1 cut(s) 141
AvaII GGWCC 1 cut(s) 133
BanI GGYRCC 1 cut(s) 93
BbsI GAAGAC 1 cut(s) 114
Bbv12I GWGCWC 1 cut(s) 254
BccI CCATC 3 cut(s) 58, 169, 215
BciT130I CCWGG 1 cut(s) 168
BcnI CCSGG 1 cut(s) 141
BfmI CTRYAG 1 cut(s) 126
BglII AGATCT 1 cut(s) 6
Bme1390I CCNGG 2 cut(s) 141, 168
Bme18I GGWCC 1 cut(s) 133
BmgT120I GGNCC 1 cut(s) 133
BmiI GGNNCC 1 cut(s) 95
BmrFI CCNGG 2 cut(s) 141, 168
BmsI GCATC 1 cut(s) 209
BpiI GAAGAC 1 cut(s) 114
BpuMI CCSGG 1 cut(s) 141
BsaAI YACGTR 1 cut(s) 35
BsaJI CCNNGG 2 cut(s) 167, 298
BsaWI WCCGGW 1 cut(s) 225
Bsc4I CCNNNNNNNGG 2 cut(s) 146, 196
Bse118I RCCGGY 1 cut(s) 86
BseAI TCCGGA 1 cut(s) 225
BseBI CCWGG 1 cut(s) 168
BseDI CCNNGG 2 cut(s) 167, 298
BseGI GGATG 1 cut(s) 177
BseLI CCNNNNNNNGG 2 cut(s) 146, 196
BseMII CTCAG 1 cut(s) 24
BshFI GGCC 2 cut(s) 206, 280
BshNI GGYRCC 1 cut(s) 93
BsiHKAI GWGCWC 1 cut(s) 254
BsiSI CCGG 3 cut(s) 87, 140, 226
BslI CCNNNNNNNGG 2 cut(s) 146, 196
BsnI GGCC 2 cut(s) 206, 280
Bsp1286I GDGCHC 1 cut(s) 254
Bsp13I TCCGGA 1 cut(s) 225
Bsp143I GATC 1 cut(s) 6
Bsp19I CCATGG 1 cut(s) 298
BspACI CCGC 2 cut(s) 90, 288
BspANI GGCC 2 cut(s) 206, 280
BspCNI CTCAG 1 cut(s) 23
BspEI TCCGGA 1 cut(s) 225
BspLI GGNNCC 1 cut(s) 95
BspT107I GGYRCC 1 cut(s) 93
BsrFI RCCGGY 1 cut(s) 86
BssAI RCCGGY 1 cut(s) 86
BssECI CCNNGG 2 cut(s) 167, 298
BssMI GATC 1 cut(s) 6
BssT1I CCWWGG 1 cut(s) 298
Bst2UI CCWGG 1 cut(s) 168
Bst4CI ACNGT 2 cut(s) 130, 256
BstBAI YACGTR 1 cut(s) 35
BstC8I GCNNGC 1 cut(s) 88
BstDEI CTNAG 1 cut(s) 10
BstDSI CCRYGG 1 cut(s) 298
BstF5I GGATG 1 cut(s) 177
BstKTI GATC 1 cut(s) 9
BstMBI GATC 1 cut(s) 6
BstMWI GCNNNNNNNGC 1 cut(s) 83
BstNI CCWGG 1 cut(s) 168
BstSCI CCNGG 2 cut(s) 139, 166
BstSFI CTRYAG 1 cut(s) 126
BstSNI TACGTA 1 cut(s) 35
BstV2I GAAGAC 1 cut(s) 114
BstX2I RGATCY 1 cut(s) 6
BstYI RGATCY 1 cut(s) 6
BsuRI GGCC 2 cut(s) 206, 280
BtgI CCRYGG 1 cut(s) 298
BtsCI GGATG 1 cut(s) 177
BtsIMutI CAGTG 2 cut(s) 18, 135
Cac8I GCNNGC 1 cut(s) 88
Cfr10I RCCGGY 1 cut(s) 86
Cfr13I GGNCC 1 cut(s) 133
Csp6I GTAC 1 cut(s) 36
CspCI CAANNNNNGTGG 2 cut(s) 223, 258
CviAII CATG 1 cut(s) 299
CviJI RGCY 5 cut(s) 86, 180, 188, 206, 280
CviKI_1 RGCY 5 cut(s) 86, 180, 188, 206, 280
CviQI GTAC 1 cut(s) 36
DdeI CTNAG 1 cut(s) 10
DpnI GATC 1 cut(s) 8
DpnII GATC 1 cut(s) 6
EaeI YGGCCR 1 cut(s) 204
Eco105I TACGTA 1 cut(s) 35
Eco130I CCWWGG 1 cut(s) 298
Eco47I GGWCC 1 cut(s) 133
EcoRII CCWGG 1 cut(s) 166
EcoT14I CCWWGG 1 cut(s) 298
ErhI CCWWGG 1 cut(s) 298
FaeI CATG 1 cut(s) 302
FaiI YATR 3 cut(s) 32, 295, 300
FatI CATG 1 cut(s) 298
FauI CCCGC 1 cut(s) 83
FblI GTMKAC 1 cut(s) 268
FokI GGATG 1 cut(s) 184
HaeIII GGCC 2 cut(s) 206, 280
HapII CCGG 3 cut(s) 87, 140, 226
Hin1II CATG 1 cut(s) 302
HincII GTYRAC 1 cut(s) 269
HindII GTYRAC 1 cut(s) 269
HinfI GANTC 1 cut(s) 116
HpaII CCGG 3 cut(s) 87, 140, 226
Hpy166II GTNNAC 2 cut(s) 56, 269
Hpy188I TCNGA 1 cut(s) 249
Hpy188III TCNNGA 2 cut(s) 107, 226
Hpy8I GTNNAC 2 cut(s) 56, 269
HpyCH4III ACNGT 2 cut(s) 130, 256
HpyCH4IV ACGT 1 cut(s) 34
HpyF10VI GCNNNNNNNGC 1 cut(s) 83
HpyF3I CTNAG 1 cut(s) 10
HpySE526I ACGT 1 cut(s) 34
Hsp92II CATG 1 cut(s) 302
Kpn2I TCCGGA 1 cut(s) 225
KroI GCCGGC 1 cut(s) 86
KroNI GCCGGC 1 cut(s) 88
Kzo9I GATC 1 cut(s) 6
LmnI GCTCC 2 cut(s) 20, 249
LpnPI CCDG 5 cut(s) 100, 153, 153, 180, 239
LweI GCATC 1 cut(s) 209
MaeII ACGT 1 cut(s) 34
MalI GATC 1 cut(s) 8
MboI GATC 1 cut(s) 6
MboII GAAGA 2 cut(s) 16, 114
MflI RGATCY 1 cut(s) 6
MhlI GDGCHC 1 cut(s) 254
MluCI AATT 1 cut(s) 70
MmeI TCCRAC 2 cut(s) 160, 227
MnlI CCTC 1 cut(s) 291
MroI TCCGGA 1 cut(s) 225
MroNI GCCGGC 1 cut(s) 86
MseI TTAA 1 cut(s) 304
MspI CCGG 3 cut(s) 87, 140, 226
MspR9I CCNGG 2 cut(s) 141, 168
MvaI CCWGG 1 cut(s) 168
MwoI GCNNNNNNNGC 1 cut(s) 83
NaeI GCCGGC 1 cut(s) 88
NciI CCSGG 1 cut(s) 141
NcoI CCATGG 1 cut(s) 298
NdeII GATC 1 cut(s) 6
NgoMIV GCCGGC 1 cut(s) 86
NlaIII CATG 1 cut(s) 302
NlaIV GGNNCC 1 cut(s) 95
PdiI GCCGGC 1 cut(s) 88
PfeI GAWTC 1 cut(s) 116
PflMI CCANNNNNTGG 1 cut(s) 196
Ppu21I YACGTR 1 cut(s) 35
Psp6I CCWGG 1 cut(s) 166
PspGI CCWGG 1 cut(s) 166
PspN4I GGNNCC 1 cut(s) 95
PspPI GGNCC 1 cut(s) 133
PsuI RGATCY 1 cut(s) 6
RsaI GTAC 1 cut(s) 37
RsaNI GTAC 1 cut(s) 36
SalI GTCGAC 1 cut(s) 267
SaqAI TTAA 1 cut(s) 304
Sau3AI GATC 1 cut(s) 6
Sau96I GGNCC 1 cut(s) 133
ScrFI CCNGG 2 cut(s) 141, 168
SduI GDGCHC 1 cut(s) 254
SetI ASST 2 cut(s) 37, 151
SfaNI GCATC 1 cut(s) 209
SfcI CTRYAG 1 cut(s) 126
SinI GGWCC 1 cut(s) 133
SnaBI TACGTA 1 cut(s) 35
Sse9I AATT 1 cut(s) 70
SsiI CCGC 2 cut(s) 90, 288
StyD4I CCNGG 2 cut(s) 139, 166
StyI CCWWGG 1 cut(s) 298
TaaI ACNGT 2 cut(s) 130, 256
TaiI ACGT 1 cut(s) 37
TaqI TCGA 2 cut(s) 114, 268
TasI AATT 1 cut(s) 70
TfiI GAWTC 1 cut(s) 116
Tru1I TTAA 1 cut(s) 304
Tru9I TTAA 1 cut(s) 304
TscAI CASTG 2 cut(s) 18, 135
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 2 cut(s) 18, 135
Van91I CCANNNNNTGG 1 cut(s) 196
VpaK11BI GGWCC 1 cut(s) 133
XmiI GTMKAC 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.