pycom08g04230

Protein of unknown function (DUF642)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0002157
Physical Location & Seq
Forward (+)
3258711 .. 3258953
243 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g04230.1

Sequence Viewer

Length: 243 bp
ATGGTCAATTTTGCCCACAAAGCCGGCGGGTGCCTAAAACTTTCCGACGTCGATTCGTCGTCTACGGTGGTCCAACGGGGTCAAGGTTGGTTGGGTTTTGCTCCTGGGATGATGGGCTACAAAGCCCAAGTGGTGGCATCGGCCATCGCTTTGCCGATTTGCCGGAAAATCGAAAAGGGTGGCCGGAGCACCATTGATTTTCGTCAACTTTCGTGGTCTCAAACCGCCGCATACCATGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF642

Protein Analysis

81

Amino Acids

8.54

Weight (kDa)

9.69

Isoelectric Point (pI)

36.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 51
AccB1I GGYRCC 1 cut(s) 30
AccB7I CCANNNNNTGG 1 cut(s) 133
AccI GTMKAC 1 cut(s) 62
AciI CCGC 3 cut(s) 27, 225, 228
AcoI YGGCCR 2 cut(s) 141, 181
AcyI GRCGYC 1 cut(s) 48
AfiI CCNNNNNNNGG 1 cut(s) 133
AjnI CCWGG 1 cut(s) 103
Alw21I GWGCWC 1 cut(s) 191
Alw26I GTCTC 1 cut(s) 222
AoxI GGCC 2 cut(s) 141, 181
AspS9I GGNCC 1 cut(s) 70
AvaII GGWCC 1 cut(s) 70
BanI GGYRCC 1 cut(s) 30
Bbv12I GWGCWC 1 cut(s) 191
BccI CCATC 2 cut(s) 106, 152
BciT130I CCWGG 1 cut(s) 105
BcoDI GTCTC 1 cut(s) 222
BisI GCNGC 1 cut(s) 228
BlsI GCNGC 1 cut(s) 229
Bme1390I CCNGG 1 cut(s) 105
Bme18I GGWCC 1 cut(s) 70
BmgT120I GGNCC 1 cut(s) 70
BmiI GGNNCC 1 cut(s) 32
BmrFI CCNGG 1 cut(s) 105
BmsI GCATC 1 cut(s) 146
BsaHI GRCGYC 1 cut(s) 48
BsaI GGTCTC 1 cut(s) 222
BsaJI CCNNGG 2 cut(s) 104, 235
Bsc4I CCNNNNNNNGG 1 cut(s) 133
Bse118I RCCGGY 1 cut(s) 23
BseBI CCWGG 1 cut(s) 105
BseDI CCNNGG 2 cut(s) 104, 235
BseGI GGATG 1 cut(s) 114
BseLI CCNNNNNNNGG 1 cut(s) 133
BshFI GGCC 2 cut(s) 143, 183
BshNI GGYRCC 1 cut(s) 30
BsiHKAI GWGCWC 1 cut(s) 191
BsiSI CCGG 3 cut(s) 24, 163, 184
BslI CCNNNNNNNGG 1 cut(s) 133
BsmAI GTCTC 1 cut(s) 222
BsnI GGCC 2 cut(s) 143, 183
Bso31I GGTCTC 1 cut(s) 222
Bsp1286I GDGCHC 1 cut(s) 191
Bsp19I CCATGG 1 cut(s) 235
BspACI CCGC 3 cut(s) 27, 225, 228
BspANI GGCC 2 cut(s) 143, 183
BspLI GGNNCC 1 cut(s) 32
BspT107I GGYRCC 1 cut(s) 30
BspTNI GGTCTC 1 cut(s) 222
BsrFI RCCGGY 1 cut(s) 23
BssAI RCCGGY 1 cut(s) 23
BssECI CCNNGG 2 cut(s) 104, 235
BssNI GRCGYC 1 cut(s) 48
BssT1I CCWWGG 1 cut(s) 235
Bst2UI CCWGG 1 cut(s) 105
Bst4CI ACNGT 1 cut(s) 67
BstACI GRCGYC 1 cut(s) 48
BstC8I GCNNGC 1 cut(s) 25
BstDSI CCRYGG 1 cut(s) 235
BstF5I GGATG 1 cut(s) 114
BstMAI GTCTC 1 cut(s) 222
BstMWI GCNNNNNNNGC 1 cut(s) 20
BstNI CCWGG 1 cut(s) 105
BstSCI CCNGG 1 cut(s) 103
BsuRI GGCC 2 cut(s) 143, 183
BtgI CCRYGG 1 cut(s) 235
BtgZI GCGATG 1 cut(s) 130
BtsCI GGATG 1 cut(s) 114
Cac8I GCNNGC 1 cut(s) 25
Cfr10I RCCGGY 1 cut(s) 23
Cfr13I GGNCC 1 cut(s) 70
CspCI CAANNNNNGTGG 2 cut(s) 194, 229
CviAII CATG 1 cut(s) 236
CviJI RGCY 5 cut(s) 23, 117, 125, 143, 183
CviKI_1 RGCY 5 cut(s) 23, 117, 125, 143, 183
EaeI YGGCCR 2 cut(s) 141, 181
Eco130I CCWWGG 1 cut(s) 235
Eco31I GGTCTC 1 cut(s) 222
Eco47I GGWCC 1 cut(s) 70
EcoRII CCWGG 1 cut(s) 103
EcoT14I CCWWGG 1 cut(s) 235
ErhI CCWWGG 1 cut(s) 235
FaeI CATG 1 cut(s) 239
FaiI YATR 2 cut(s) 232, 237
FatI CATG 1 cut(s) 235
FauI CCCGC 1 cut(s) 20
FblI GTMKAC 1 cut(s) 62
Fnu4HI GCNGC 1 cut(s) 228
FokI GGATG 1 cut(s) 121
Fsp4HI GCNGC 1 cut(s) 228
GluI GCNGC 1 cut(s) 228
HaeIII GGCC 2 cut(s) 143, 183
HapII CCGG 3 cut(s) 24, 163, 184
Hin1I GRCGYC 1 cut(s) 48
Hin1II CATG 1 cut(s) 239
HincII GTYRAC 1 cut(s) 206
HindII GTYRAC 1 cut(s) 206
HinfI GANTC 1 cut(s) 53
HpaII CCGG 3 cut(s) 24, 163, 184
Hpy166II GTNNAC 2 cut(s) 63, 206
Hpy188I TCNGA 1 cut(s) 46
Hpy8I GTNNAC 2 cut(s) 63, 206
Hpy99I CGWCG 3 cut(s) 50, 53, 61
HpyCH4III ACNGT 1 cut(s) 67
HpyCH4IV ACGT 1 cut(s) 48
HpyF10VI GCNNNNNNNGC 1 cut(s) 20
HpySE526I ACGT 1 cut(s) 48
Hsp92I GRCGYC 1 cut(s) 48
Hsp92II CATG 1 cut(s) 239
KroI GCCGGC 1 cut(s) 23
KroNI GCCGGC 1 cut(s) 25
LmnI GCTCC 2 cut(s) 106, 186
LpnPI CCDG 5 cut(s) 37, 90, 117, 176, 197
LweI GCATC 1 cut(s) 146
MaeII ACGT 1 cut(s) 48
MhlI GDGCHC 1 cut(s) 191
MluCI AATT 1 cut(s) 7
MmeI TCCRAC 2 cut(s) 69, 97
MroNI GCCGGC 1 cut(s) 23
MseI TTAA 1 cut(s) 241
MspI CCGG 3 cut(s) 24, 163, 184
MspR9I CCNGG 1 cut(s) 105
MvaI CCWGG 1 cut(s) 105
MwoI GCNNNNNNNGC 1 cut(s) 20
NaeI GCCGGC 1 cut(s) 25
NcoI CCATGG 1 cut(s) 235
NgoMIV GCCGGC 1 cut(s) 23
NlaIII CATG 1 cut(s) 239
NlaIV GGNNCC 1 cut(s) 32
PdiI GCCGGC 1 cut(s) 25
PfeI GAWTC 1 cut(s) 53
PflMI CCANNNNNTGG 1 cut(s) 133
PkrI GCNGC 1 cut(s) 229
Psp6I CCWGG 1 cut(s) 103
PspGI CCWGG 1 cut(s) 103
PspN4I GGNNCC 1 cut(s) 32
PspPI GGNCC 1 cut(s) 70
SaqAI TTAA 1 cut(s) 241
SatI GCNGC 1 cut(s) 228
Sau96I GGNCC 1 cut(s) 70
ScrFI CCNGG 1 cut(s) 105
SduI GDGCHC 1 cut(s) 191
SetI ASST 2 cut(s) 51, 88
SfaNI GCATC 1 cut(s) 146
SinI GGWCC 1 cut(s) 70
Sse9I AATT 1 cut(s) 7
SsiI CCGC 3 cut(s) 27, 225, 228
StyD4I CCNGG 1 cut(s) 103
StyI CCWWGG 1 cut(s) 235
TaaI ACNGT 1 cut(s) 67
TaiI ACGT 1 cut(s) 51
TaqI TCGA 2 cut(s) 51, 171
TasI AATT 1 cut(s) 7
TauI GCSGC 1 cut(s) 230
TfiI GAWTC 1 cut(s) 53
Tru1I TTAA 1 cut(s) 241
Tru9I TTAA 1 cut(s) 241
Van91I CCANNNNNTGG 1 cut(s) 133
VpaK11BI GGWCC 1 cut(s) 70
XmiI GTMKAC 1 cut(s) 62
ZraI GACGTC 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.