pycom09g13460

Protein of unknown function (DUF642)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0002157
Physical Location & Seq
Forward (+)
12259033 .. 12259991
959 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g13460.1

Sequence Viewer

Length: 273 bp
ATGATTGGTCTCTGTGCCACGAAGTCCAAAAGTGGACGGAAGATGGTCAATTTTGCCCACAAAGCCGGCGGGTGCCTAAAACTTCCCGACGTCGATTCGTCGTCTACGGTGGTCCAACGGGGTCAAGGTTGGTTGGGTTTTTCTCCTGGGATGATGGGCTACAAAGCCCAAGTGGTGGCATCGGCCATCGCTTTGCCGATTTGCCGGAAAATCGAAAAGGGTGGCCGGATTCGTCAACTTTCGTGGCCTCAAACCACCTCATACCATGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF642

Protein Analysis

91

Amino Acids

9.64

Weight (kDa)

10.19

Isoelectric Point (pI)

36.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 93
AccB1I GGYRCC 1 cut(s) 72
AccB7I CCANNNNNTGG 1 cut(s) 175
AccI GTMKAC 1 cut(s) 104
AciI CCGC 1 cut(s) 69
AcoI YGGCCR 2 cut(s) 183, 223
AcyI GRCGYC 1 cut(s) 90
AfiI CCNNNNNNNGG 1 cut(s) 175
AjnI CCWGG 1 cut(s) 145
Alw26I GTCTC 1 cut(s) 14
AoxI GGCC 3 cut(s) 183, 223, 245
AspS9I GGNCC 1 cut(s) 112
AvaII GGWCC 1 cut(s) 112
BanI GGYRCC 1 cut(s) 72
BccI CCATC 3 cut(s) 37, 148, 194
BciT130I CCWGG 1 cut(s) 147
BcoDI GTCTC 1 cut(s) 14
Bme1390I CCNGG 1 cut(s) 147
Bme18I GGWCC 1 cut(s) 112
BmgT120I GGNCC 1 cut(s) 112
BmiI GGNNCC 1 cut(s) 74
BmrFI CCNGG 1 cut(s) 147
BmsI GCATC 1 cut(s) 188
BsaHI GRCGYC 1 cut(s) 90
BsaI GGTCTC 1 cut(s) 14
BsaJI CCNNGG 2 cut(s) 146, 265
Bsc4I CCNNNNNNNGG 1 cut(s) 175
Bse118I RCCGGY 1 cut(s) 65
BseBI CCWGG 1 cut(s) 147
BseDI CCNNGG 2 cut(s) 146, 265
BseGI GGATG 1 cut(s) 156
BseLI CCNNNNNNNGG 1 cut(s) 175
BshFI GGCC 3 cut(s) 185, 225, 247
BshNI GGYRCC 1 cut(s) 72
BsiSI CCGG 3 cut(s) 66, 205, 226
BslI CCNNNNNNNGG 1 cut(s) 175
BsmAI GTCTC 1 cut(s) 14
BsnI GGCC 3 cut(s) 185, 225, 247
Bso31I GGTCTC 1 cut(s) 14
Bsp19I CCATGG 1 cut(s) 265
BspACI CCGC 1 cut(s) 69
BspANI GGCC 3 cut(s) 185, 225, 247
BspLI GGNNCC 1 cut(s) 74
BspT107I GGYRCC 1 cut(s) 72
BspTNI GGTCTC 1 cut(s) 14
BsrFI RCCGGY 1 cut(s) 65
BssAI RCCGGY 1 cut(s) 65
BssECI CCNNGG 2 cut(s) 146, 265
BssNI GRCGYC 1 cut(s) 90
BssT1I CCWWGG 1 cut(s) 265
Bst2UI CCWGG 1 cut(s) 147
Bst4CI ACNGT 1 cut(s) 109
BstACI GRCGYC 1 cut(s) 90
BstC8I GCNNGC 1 cut(s) 67
BstDSI CCRYGG 1 cut(s) 265
BstF5I GGATG 1 cut(s) 156
BstMAI GTCTC 1 cut(s) 14
BstMWI GCNNNNNNNGC 1 cut(s) 62
BstNI CCWGG 1 cut(s) 147
BstSCI CCNGG 1 cut(s) 145
BsuRI GGCC 3 cut(s) 185, 225, 247
BtgI CCRYGG 1 cut(s) 265
BtgZI GCGATG 1 cut(s) 172
BtsCI GGATG 1 cut(s) 156
Cac8I GCNNGC 1 cut(s) 67
Cfr10I RCCGGY 1 cut(s) 65
Cfr13I GGNCC 1 cut(s) 112
CspCI CAANNNNNGTGG 2 cut(s) 224, 259
CviAII CATG 1 cut(s) 266
CviJI RGCY 6 cut(s) 65, 159, 167, 185, 225, 247
CviKI_1 RGCY 6 cut(s) 65, 159, 167, 185, 225, 247
EaeI YGGCCR 2 cut(s) 183, 223
Eco130I CCWWGG 1 cut(s) 265
Eco31I GGTCTC 1 cut(s) 14
Eco47I GGWCC 1 cut(s) 112
EcoRII CCWGG 1 cut(s) 145
EcoT14I CCWWGG 1 cut(s) 265
ErhI CCWWGG 1 cut(s) 265
FaeI CATG 1 cut(s) 269
FaiI YATR 2 cut(s) 262, 267
FatI CATG 1 cut(s) 265
FauI CCCGC 1 cut(s) 62
FblI GTMKAC 1 cut(s) 104
FokI GGATG 1 cut(s) 163
HaeIII GGCC 3 cut(s) 185, 225, 247
HapII CCGG 3 cut(s) 66, 205, 226
Hin1I GRCGYC 1 cut(s) 90
Hin1II CATG 1 cut(s) 269
HincII GTYRAC 1 cut(s) 236
HindII GTYRAC 1 cut(s) 236
HinfI GANTC 2 cut(s) 95, 229
HpaII CCGG 3 cut(s) 66, 205, 226
Hpy166II GTNNAC 3 cut(s) 35, 105, 236
Hpy188III TCNNGA 1 cut(s) 86
Hpy8I GTNNAC 3 cut(s) 35, 105, 236
Hpy99I CGWCG 3 cut(s) 92, 95, 103
HpyCH4III ACNGT 1 cut(s) 109
HpyCH4IV ACGT 1 cut(s) 90
HpyF10VI GCNNNNNNNGC 1 cut(s) 62
HpySE526I ACGT 1 cut(s) 90
Hsp92I GRCGYC 1 cut(s) 90
Hsp92II CATG 1 cut(s) 269
KroI GCCGGC 1 cut(s) 65
KroNI GCCGGC 1 cut(s) 67
LpnPI CCDG 5 cut(s) 79, 132, 159, 218, 239
LweI GCATC 1 cut(s) 188
MaeII ACGT 1 cut(s) 90
MboII GAAGA 1 cut(s) 52
MluCI AATT 1 cut(s) 49
MmeI TCCRAC 1 cut(s) 139
MnlI CCTC 2 cut(s) 258, 268
MroNI GCCGGC 1 cut(s) 65
MseI TTAA 1 cut(s) 271
MspI CCGG 3 cut(s) 66, 205, 226
MspR9I CCNGG 1 cut(s) 147
MvaI CCWGG 1 cut(s) 147
MwoI GCNNNNNNNGC 1 cut(s) 62
NaeI GCCGGC 1 cut(s) 67
NcoI CCATGG 1 cut(s) 265
NgoMIV GCCGGC 1 cut(s) 65
NlaIII CATG 1 cut(s) 269
NlaIV GGNNCC 1 cut(s) 74
PdiI GCCGGC 1 cut(s) 67
PfeI GAWTC 2 cut(s) 95, 229
PflMI CCANNNNNTGG 1 cut(s) 175
Psp6I CCWGG 1 cut(s) 145
PspGI CCWGG 1 cut(s) 145
PspN4I GGNNCC 1 cut(s) 74
PspPI GGNCC 1 cut(s) 112
SaqAI TTAA 1 cut(s) 271
Sau96I GGNCC 1 cut(s) 112
ScrFI CCNGG 1 cut(s) 147
SetI ASST 3 cut(s) 93, 130, 260
SfaNI GCATC 1 cut(s) 188
SinI GGWCC 1 cut(s) 112
Sse9I AATT 1 cut(s) 49
SsiI CCGC 1 cut(s) 69
StyD4I CCNGG 1 cut(s) 145
StyI CCWWGG 1 cut(s) 265
TaaI ACNGT 1 cut(s) 109
TaiI ACGT 1 cut(s) 93
TaqI TCGA 2 cut(s) 93, 213
TasI AATT 1 cut(s) 49
TfiI GAWTC 2 cut(s) 95, 229
Tru1I TTAA 1 cut(s) 271
Tru9I TTAA 1 cut(s) 271
TspGWI ACGGA 1 cut(s) 52
Van91I CCANNNNNTGG 1 cut(s) 175
VpaK11BI GGWCC 1 cut(s) 112
XmiI GTMKAC 1 cut(s) 104
ZraI GACGTC 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.