pycom14g16640

Protein of unknown function (DUF642)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0002157
Physical Location & Seq
Reverse (-)
18861490 .. 18861795
306 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g16640.1

Sequence Viewer

Length: 306 bp
ATGAAGATCTCAGTGAGTGGAGCAACTTTCATACCTGCCACGAAGTCCAAAAGTGGACGGAAGATGGTCAATTTTGTCCACAAAGCCGGCGGGTGCCTAAAACTTCCTGACGTCGATTTGTCGTCTACGGTGGTCCAACGGGGTCAAGGTTGGTTGGGTTTTGCTCCTGGGATGATGGGATACAAAGCCCAAGTGGTGGCATCGGTCATCGCTTTGCCGATTTGCCGGAAAATCGAAAAGGGTGGCCGGAGCACCATTGATTTTCGTCAACTTTCGTGGCCTCAAACCGCCCCATACCATAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF642

Protein Analysis

102

Amino Acids

10.88

Weight (kDa)

10.25

Isoelectric Point (pI)

36.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 114
Acc36I ACCTGC 1 cut(s) 43
AccB1I GGYRCC 1 cut(s) 93
AccB7I CCANNNNNTGG 1 cut(s) 196
AccI GTMKAC 1 cut(s) 125
AciI CCGC 2 cut(s) 90, 288
AcoI YGGCCR 1 cut(s) 244
AcyI GRCGYC 1 cut(s) 111
AfiI CCNNNNNNNGG 1 cut(s) 196
AjnI CCWGG 1 cut(s) 166
Alw21I GWGCWC 1 cut(s) 254
AoxI GGCC 2 cut(s) 244, 278
AspS9I GGNCC 1 cut(s) 133
AvaII GGWCC 1 cut(s) 133
BanI GGYRCC 1 cut(s) 93
Bbv12I GWGCWC 1 cut(s) 254
BccI CCATC 2 cut(s) 58, 169
BciT130I CCWGG 1 cut(s) 168
BciVI GTATCC 1 cut(s) 173
BfuAI ACCTGC 1 cut(s) 43
BfuI GTATCC 1 cut(s) 173
BglII AGATCT 1 cut(s) 6
Bme1390I CCNGG 1 cut(s) 168
Bme18I GGWCC 1 cut(s) 133
BmgT120I GGNCC 1 cut(s) 133
BmiI GGNNCC 1 cut(s) 95
BmrFI CCNGG 1 cut(s) 168
BmsI GCATC 1 cut(s) 209
BsaHI GRCGYC 1 cut(s) 111
BsaJI CCNNGG 1 cut(s) 167
Bsc4I CCNNNNNNNGG 1 cut(s) 196
Bse118I RCCGGY 1 cut(s) 86
BseBI CCWGG 1 cut(s) 168
BseDI CCNNGG 1 cut(s) 167
BseGI GGATG 1 cut(s) 177
BseLI CCNNNNNNNGG 1 cut(s) 196
BseMII CTCAG 1 cut(s) 24
BshFI GGCC 2 cut(s) 246, 280
BshNI GGYRCC 1 cut(s) 93
BsiHKAI GWGCWC 1 cut(s) 254
BsiSI CCGG 3 cut(s) 87, 226, 247
BslI CCNNNNNNNGG 1 cut(s) 196
BsnI GGCC 2 cut(s) 246, 280
Bsp1286I GDGCHC 1 cut(s) 254
Bsp143I GATC 1 cut(s) 6
BspACI CCGC 2 cut(s) 90, 288
BspANI GGCC 2 cut(s) 246, 280
BspCNI CTCAG 1 cut(s) 23
BspLI GGNNCC 1 cut(s) 95
BspMI ACCTGC 1 cut(s) 43
BspT107I GGYRCC 1 cut(s) 93
BsrFI RCCGGY 1 cut(s) 86
BssAI RCCGGY 1 cut(s) 86
BssECI CCNNGG 1 cut(s) 167
BssMI GATC 1 cut(s) 6
BssNI GRCGYC 1 cut(s) 111
Bst2UI CCWGG 1 cut(s) 168
Bst4CI ACNGT 1 cut(s) 130
BstACI GRCGYC 1 cut(s) 111
BstC8I GCNNGC 1 cut(s) 88
BstDEI CTNAG 1 cut(s) 10
BstF5I GGATG 1 cut(s) 177
BstKTI GATC 1 cut(s) 9
BstMBI GATC 1 cut(s) 6
BstNI CCWGG 1 cut(s) 168
BstSCI CCNGG 1 cut(s) 166
BstX2I RGATCY 1 cut(s) 6
BstYI RGATCY 1 cut(s) 6
BsuI GTATCC 1 cut(s) 173
BsuRI GGCC 2 cut(s) 246, 280
BtgZI GCGATG 1 cut(s) 193
BtsCI GGATG 1 cut(s) 177
BtsIMutI CAGTG 1 cut(s) 18
BveI ACCTGC 1 cut(s) 43
Cac8I GCNNGC 1 cut(s) 88
Cfr10I RCCGGY 1 cut(s) 86
Cfr13I GGNCC 1 cut(s) 133
CspCI CAANNNNNGTGG 2 cut(s) 257, 292
CviJI RGCY 4 cut(s) 86, 188, 246, 280
CviKI_1 RGCY 4 cut(s) 86, 188, 246, 280
DdeI CTNAG 1 cut(s) 10
DpnI GATC 1 cut(s) 8
DpnII GATC 1 cut(s) 6
EaeI YGGCCR 1 cut(s) 244
Eco47I GGWCC 1 cut(s) 133
EcoRII CCWGG 1 cut(s) 166
FaiI YATR 3 cut(s) 32, 295, 300
FauI CCCGC 1 cut(s) 83
FblI GTMKAC 1 cut(s) 125
FokI GGATG 1 cut(s) 184
HaeIII GGCC 2 cut(s) 246, 280
HapII CCGG 3 cut(s) 87, 226, 247
Hin1I GRCGYC 1 cut(s) 111
HincII GTYRAC 1 cut(s) 269
HindII GTYRAC 1 cut(s) 269
HpaII CCGG 3 cut(s) 87, 226, 247
Hpy166II GTNNAC 4 cut(s) 56, 79, 126, 269
Hpy188III TCNNGA 1 cut(s) 107
Hpy8I GTNNAC 4 cut(s) 56, 79, 126, 269
Hpy99I CGWCG 1 cut(s) 116
HpyCH4III ACNGT 1 cut(s) 130
HpyCH4IV ACGT 1 cut(s) 111
HpyF3I CTNAG 1 cut(s) 10
HpySE526I ACGT 1 cut(s) 111
Hsp92I GRCGYC 1 cut(s) 111
KroI GCCGGC 1 cut(s) 86
KroNI GCCGGC 1 cut(s) 88
Kzo9I GATC 1 cut(s) 6
LmnI GCTCC 3 cut(s) 20, 169, 249
LpnPI CCDG 7 cut(s) 48, 100, 120, 153, 180, 239, 260
LweI GCATC 1 cut(s) 209
MaeII ACGT 1 cut(s) 111
MalI GATC 1 cut(s) 8
MboI GATC 1 cut(s) 6
MboII GAAGA 2 cut(s) 16, 73
MflI RGATCY 1 cut(s) 6
MhlI GDGCHC 1 cut(s) 254
MluCI AATT 1 cut(s) 70
MmeI TCCRAC 1 cut(s) 160
MnlI CCTC 1 cut(s) 291
MroNI GCCGGC 1 cut(s) 86
MseI TTAA 1 cut(s) 304
MspI CCGG 3 cut(s) 87, 226, 247
MspR9I CCNGG 1 cut(s) 168
MvaI CCWGG 1 cut(s) 168
NaeI GCCGGC 1 cut(s) 88
NdeII GATC 1 cut(s) 6
NgoMIV GCCGGC 1 cut(s) 86
NlaIV GGNNCC 1 cut(s) 95
PdiI GCCGGC 1 cut(s) 88
PflMI CCANNNNNTGG 1 cut(s) 196
Psp6I CCWGG 1 cut(s) 166
PspGI CCWGG 1 cut(s) 166
PspN4I GGNNCC 1 cut(s) 95
PspPI GGNCC 1 cut(s) 133
PsuI RGATCY 1 cut(s) 6
SaqAI TTAA 1 cut(s) 304
Sau3AI GATC 1 cut(s) 6
Sau96I GGNCC 1 cut(s) 133
ScrFI CCNGG 1 cut(s) 168
SduI GDGCHC 1 cut(s) 254
SetI ASST 3 cut(s) 37, 114, 151
SfaNI GCATC 1 cut(s) 209
SinI GGWCC 1 cut(s) 133
Sse9I AATT 1 cut(s) 70
SsiI CCGC 2 cut(s) 90, 288
StyD4I CCNGG 1 cut(s) 166
TaaI ACNGT 1 cut(s) 130
TaiI ACGT 1 cut(s) 114
TaqI TCGA 2 cut(s) 114, 234
TaqII GACCGA 1 cut(s) 193
TasI AATT 1 cut(s) 70
Tru1I TTAA 1 cut(s) 304
Tru9I TTAA 1 cut(s) 304
TscAI CASTG 1 cut(s) 18
TspDTI ATGAA 2 cut(s) 17, 19
TspGWI ACGGA 1 cut(s) 73
TspRI CASTG 1 cut(s) 18
Van91I CCANNNNNTGG 1 cut(s) 196
VpaK11BI GGWCC 1 cut(s) 133
XmiI GTMKAC 1 cut(s) 125
ZraI GACGTC 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.