pycom04g10700

Protein of unknown function (DUF642)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0002157
Physical Location & Seq
Reverse (-)
13451158 .. 13451717
560 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g10700.1

Sequence Viewer

Length: 465 bp
ATGTATCCAAGTTTGGTGACGATCCAACGTCAAGACATTCGACATAGCCTAAAAATAGCATTGGCGGCCCACTGGCCATGCACCACCGCGGGTGGCGGTCGGCCACCCCGACTCGCCGGAAAATCCAACTATTTCCAAAAATTCTCAAACTTCACAGAAATGAAGACCTCAGTGAGTGGAGAAACTTTCATACTTGCCACGAAGTCCAAAAGTGGACGGAAGATGGTCAATTTTGCCCACAAAGCCGGCGGGTGCCTAAAACTTCCCGACGTCGATTCGTCGTCTACGGTGGTCCAACGGGGTCAAGGTTGGTTGGGTTTTGCTCCTGGGATGATGGGCTACAAAGCCCAAGTGGTGGCATCGGCCATCGCTTTGCCAATTTGCCGGAAAATCGAAAAGGGTGGCCGGAGCACTATTGATTTTCATCAACTTTCGTGGCCTCAAACCGCCACATCCCATGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF642

Protein Analysis

155

Amino Acids

16.68

Weight (kDa)

10.18

Isoelectric Point (pI)

35.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 273
AccB1I GGYRCC 1 cut(s) 252
AccB7I CCANNNNNTGG 1 cut(s) 355
AccI GTMKAC 1 cut(s) 284
AccII CGCG 1 cut(s) 89
AciI CCGC 6 cut(s) 65, 87, 89, 96, 249, 447
AclWI GGATC 1 cut(s) 16
AcoI YGGCCR 4 cut(s) 74, 101, 363, 403
AcsI RAATTY 1 cut(s) 140
AcyI GRCGYC 1 cut(s) 270
AfiI CCNNNNNNNGG 1 cut(s) 355
AjnI CCWGG 1 cut(s) 325
Alw21I GWGCWC 1 cut(s) 413
AlwI GGATC 1 cut(s) 16
AoxI GGCC 6 cut(s) 66, 74, 101, 363, 403, 437
ApoI RAATTY 1 cut(s) 140
AspS9I GGNCC 2 cut(s) 67, 292
AsuHPI GGTGA 1 cut(s) 28
AvaII GGWCC 1 cut(s) 292
BalI TGGCCA 1 cut(s) 76
BanI GGYRCC 1 cut(s) 252
BbsI GAAGAC 1 cut(s) 170
Bbv12I GWGCWC 1 cut(s) 413
BccI CCATC 3 cut(s) 217, 328, 374
BciT130I CCWGG 1 cut(s) 327
BciVI GTATCC 1 cut(s) 15
BfuI GTATCC 1 cut(s) 15
BisI GCNGC 1 cut(s) 66
BlsI GCNGC 1 cut(s) 67
Bme1390I CCNGG 1 cut(s) 327
Bme18I GGWCC 1 cut(s) 292
BmgT120I GGNCC 2 cut(s) 67, 292
BmiI GGNNCC 1 cut(s) 254
BmrFI CCNGG 1 cut(s) 327
BmsI GCATC 1 cut(s) 368
BpiI GAAGAC 1 cut(s) 170
BsaBI GATNNNNATC 1 cut(s) 423
BsaHI GRCGYC 1 cut(s) 270
BsaJI CCNNGG 3 cut(s) 87, 326, 457
Bsc4I CCNNNNNNNGG 1 cut(s) 355
Bse118I RCCGGY 1 cut(s) 245
Bse1I ACTGG 1 cut(s) 77
Bse8I GATNNNNATC 1 cut(s) 423
BseBI CCWGG 1 cut(s) 327
BseDI CCNNGG 3 cut(s) 87, 326, 457
BseGI GGATG 2 cut(s) 336, 452
BseJI GATNNNNATC 1 cut(s) 423
BseLI CCNNNNNNNGG 1 cut(s) 355
BseMII CTCAG 1 cut(s) 183
BseNI ACTGG 1 cut(s) 77
Bsh1236I CGCG 1 cut(s) 89
Bsh1285I CGRYCG 1 cut(s) 100
BshFI GGCC 6 cut(s) 68, 76, 103, 365, 405, 439
BshNI GGYRCC 1 cut(s) 252
BsiEI CGRYCG 1 cut(s) 100
BsiHKAI GWGCWC 1 cut(s) 413
BsiSI CCGG 4 cut(s) 117, 246, 385, 406
BslI CCNNNNNNNGG 1 cut(s) 355
BsnI GGCC 6 cut(s) 68, 76, 103, 365, 405, 439
Bsp1286I GDGCHC 1 cut(s) 413
Bsp143I GATC 1 cut(s) 21
Bsp19I CCATGG 1 cut(s) 457
BspACI CCGC 6 cut(s) 65, 87, 89, 96, 249, 447
BspANI GGCC 6 cut(s) 68, 76, 103, 365, 405, 439
BspCNI CTCAG 1 cut(s) 182
BspFNI CGCG 1 cut(s) 89
BspLI GGNNCC 1 cut(s) 254
BspPI GGATC 1 cut(s) 16
BspT107I GGYRCC 1 cut(s) 252
BsrFI RCCGGY 1 cut(s) 245
BsrI ACTGG 1 cut(s) 77
BssAI RCCGGY 1 cut(s) 245
BssECI CCNNGG 3 cut(s) 87, 326, 457
BssMI GATC 1 cut(s) 21
BssNI GRCGYC 1 cut(s) 270
BssT1I CCWWGG 1 cut(s) 457
Bst2UI CCWGG 1 cut(s) 327
Bst4CI ACNGT 1 cut(s) 289
BstACI GRCGYC 1 cut(s) 270
BstC8I GCNNGC 1 cut(s) 247
BstDEI CTNAG 1 cut(s) 169
BstDSI CCRYGG 2 cut(s) 87, 457
BstF5I GGATG 2 cut(s) 336, 452
BstFNI CGCG 1 cut(s) 89
BstKTI GATC 1 cut(s) 24
BstMBI GATC 1 cut(s) 21
BstMCI CGRYCG 1 cut(s) 100
BstMWI GCNNNNNNNGC 2 cut(s) 65, 242
BstNI CCWGG 1 cut(s) 327
BstSCI CCNGG 1 cut(s) 325
BstUI CGCG 1 cut(s) 89
BstV2I GAAGAC 1 cut(s) 170
BsuI GTATCC 1 cut(s) 15
BsuRI GGCC 6 cut(s) 68, 76, 103, 365, 405, 439
BtgI CCRYGG 2 cut(s) 87, 457
BtgZI GCGATG 1 cut(s) 352
BtsCI GGATG 2 cut(s) 336, 452
BtsIMutI CAGTG 2 cut(s) 70, 177
Cac8I GCNNGC 1 cut(s) 247
Cfr10I RCCGGY 1 cut(s) 245
Cfr13I GGNCC 2 cut(s) 67, 292
Cfr42I CCGCGG 1 cut(s) 90
CspCI CAANNNNNGTGG 2 cut(s) 416, 451
CviAII CATG 2 cut(s) 78, 458
DdeI CTNAG 1 cut(s) 169
DpnI GATC 1 cut(s) 23
DpnII GATC 1 cut(s) 21
EaeI YGGCCR 4 cut(s) 74, 101, 363, 403
Eco130I CCWWGG 1 cut(s) 457
Eco47I GGWCC 1 cut(s) 292
EcoRII CCWGG 1 cut(s) 325
EcoT14I CCWWGG 1 cut(s) 457
ErhI CCWWGG 1 cut(s) 457
FaeI CATG 2 cut(s) 81, 461
FaiI YATR 4 cut(s) 45, 79, 191, 459
FatI CATG 2 cut(s) 77, 457
FauI CCCGC 2 cut(s) 82, 242
FblI GTMKAC 1 cut(s) 284
Fnu4HI GCNGC 1 cut(s) 66
FokI GGATG 2 cut(s) 343, 439
Fsp4HI GCNGC 1 cut(s) 66
GluI GCNGC 1 cut(s) 66
HaeIII GGCC 6 cut(s) 68, 76, 103, 365, 405, 439
HapII CCGG 4 cut(s) 117, 246, 385, 406
Hin1I GRCGYC 1 cut(s) 270
Hin1II CATG 2 cut(s) 81, 461
HinfI GANTC 2 cut(s) 111, 275
HpaII CCGG 4 cut(s) 117, 246, 385, 406
HphI GGTGA 1 cut(s) 28
Hpy166II GTNNAC 2 cut(s) 215, 285
Hpy188III TCNNGA 2 cut(s) 32, 266
Hpy8I GTNNAC 2 cut(s) 215, 285
Hpy99I CGWCG 3 cut(s) 272, 275, 283
HpyCH4III ACNGT 1 cut(s) 289
HpyCH4IV ACGT 2 cut(s) 28, 270
HpyCH4V TGCA 1 cut(s) 81
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 242
HpyF3I CTNAG 1 cut(s) 169
HpySE526I ACGT 2 cut(s) 28, 270
Hsp92I GRCGYC 1 cut(s) 270
Hsp92II CATG 2 cut(s) 81, 461
KroI GCCGGC 1 cut(s) 245
KroNI GCCGGC 1 cut(s) 247
KspI CCGCGG 1 cut(s) 90
Kzo9I GATC 1 cut(s) 21
LmnI GCTCC 2 cut(s) 328, 408
LpnPI CCDG 7 cut(s) 58, 130, 259, 312, 339, 398, 419
LweI GCATC 1 cut(s) 368
MaeII ACGT 2 cut(s) 28, 270
MaeIII GTNAC 1 cut(s) 16
MalI GATC 1 cut(s) 23
MboI GATC 1 cut(s) 21
MboII GAAGA 2 cut(s) 175, 232
MhlI GDGCHC 1 cut(s) 413
MlsI TGGCCA 1 cut(s) 76
MluCI AATT 3 cut(s) 140, 229, 378
MluNI TGGCCA 1 cut(s) 76
MlyI GAGTC 1 cut(s) 105
MmeI TCCRAC 3 cut(s) 49, 150, 319
MnlI CCTC 2 cut(s) 178, 450
Mox20I TGGCCA 1 cut(s) 76
MroNI GCCGGC 1 cut(s) 245
MscI TGGCCA 1 cut(s) 76
MseI TTAA 1 cut(s) 463
MslI CAYNNNNRTG 1 cut(s) 158
Msp20I TGGCCA 1 cut(s) 76
MspA1I CMGCKG 1 cut(s) 89
MspI CCGG 4 cut(s) 117, 246, 385, 406
MspR9I CCNGG 1 cut(s) 327
MvaI CCWGG 1 cut(s) 327
MvnI CGCG 1 cut(s) 89
MwoI GCNNNNNNNGC 2 cut(s) 65, 242
NaeI GCCGGC 1 cut(s) 247
NcoI CCATGG 1 cut(s) 457
NdeII GATC 1 cut(s) 21
NgoMIV GCCGGC 1 cut(s) 245
NlaIII CATG 2 cut(s) 81, 461
NlaIV GGNNCC 1 cut(s) 254
NmuCI GTSAC 1 cut(s) 16
PcsI WCGNNNNNNNCGW 1 cut(s) 106
PdiI GCCGGC 1 cut(s) 247
PfeI GAWTC 1 cut(s) 275
PflMI CCANNNNNTGG 1 cut(s) 355
PkrI GCNGC 1 cut(s) 67
PleI GAGTC 1 cut(s) 105
PpsI GAGTC 1 cut(s) 105
Psp6I CCWGG 1 cut(s) 325
PspGI CCWGG 1 cut(s) 325
PspN4I GGNNCC 1 cut(s) 254
PspPI GGNCC 2 cut(s) 67, 292
RseI CAYNNNNRTG 1 cut(s) 158
SacII CCGCGG 1 cut(s) 90
SaqAI TTAA 1 cut(s) 463
SatI GCNGC 1 cut(s) 66
Sau3AI GATC 1 cut(s) 21
Sau96I GGNCC 2 cut(s) 67, 292
SchI GAGTC 1 cut(s) 105
ScrFI CCNGG 1 cut(s) 327
SduI GDGCHC 1 cut(s) 413
SetI ASST 4 cut(s) 31, 170, 273, 310
SfaNI GCATC 1 cut(s) 368
Sfr303I CCGCGG 1 cut(s) 90
SgrBI CCGCGG 1 cut(s) 90
SinI GGWCC 1 cut(s) 292
SmiMI CAYNNNNRTG 1 cut(s) 158
Sse9I AATT 3 cut(s) 140, 229, 378
SsiI CCGC 6 cut(s) 65, 87, 89, 96, 249, 447
StyD4I CCNGG 1 cut(s) 325
StyI CCWWGG 1 cut(s) 457
TaaI ACNGT 1 cut(s) 289
TaiI ACGT 2 cut(s) 31, 273
TaqI TCGA 3 cut(s) 40, 273, 393
TasI AATT 3 cut(s) 140, 229, 378
TauI GCSGC 1 cut(s) 68
TfiI GAWTC 1 cut(s) 275
Tru1I TTAA 1 cut(s) 463
Tru9I TTAA 1 cut(s) 463
TscAI CASTG 2 cut(s) 77, 177
TseFI GTSAC 1 cut(s) 16
Tsp45I GTSAC 1 cut(s) 16
TspDTI ATGAA 3 cut(s) 176, 178, 413
TspGWI ACGGA 1 cut(s) 232
TspRI CASTG 2 cut(s) 77, 177
Van91I CCANNNNNTGG 1 cut(s) 355
VpaK11BI GGWCC 1 cut(s) 292
XapI RAATTY 1 cut(s) 140
XmiI GTMKAC 1 cut(s) 284
ZraI GACGTC 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.