pycom07g20810

Protein of unknown function (DUF642)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0002157
Physical Location & Seq
Reverse (-)
22780456 .. 22780780
325 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g20810.2

Sequence Viewer

Length: 285 bp
ATGAAGATCTCTGTGAGTGGAGCAACTTTCATATCTGCCACGAACGTCAATTTTGCCCATAAAGCCGGCGGGTGCCTAAAACTTCCCGACGTCAATTCGTCATCTACGGTGGTCCAACGGGGTCAAGGTTGGTTGGGTTTTGCTCTTGGGATGATGGGCTACAAAGCCCAAGTGGTGGCATCAGCCATCGCTTTGCCGATTTGCCAGAAAATCGAAAAGGGTGGCCGGAGCACTATTGATTTTCGTCAAATTTCGTGGCCTCAAACCGCCACATTTCATGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF642

Protein Analysis

95

Amino Acids

9.94

Weight (kDa)

9.92

Isoelectric Point (pI)

28.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 93
AccB1I GGYRCC 1 cut(s) 72
AccB7I CCANNNNNTGG 1 cut(s) 175
AciI CCGC 2 cut(s) 69, 267
AcoI YGGCCR 1 cut(s) 223
AcsI RAATTY 1 cut(s) 249
AcyI GRCGYC 1 cut(s) 90
AfiI CCNNNNNNNGG 1 cut(s) 175
Alw21I GWGCWC 1 cut(s) 233
AoxI GGCC 2 cut(s) 223, 257
ApoI RAATTY 1 cut(s) 249
AspS9I GGNCC 1 cut(s) 112
AvaII GGWCC 1 cut(s) 112
BanI GGYRCC 1 cut(s) 72
Bbv12I GWGCWC 1 cut(s) 233
BccI CCATC 2 cut(s) 148, 194
BglII AGATCT 1 cut(s) 6
Bme18I GGWCC 1 cut(s) 112
BmgT120I GGNCC 1 cut(s) 112
BmiI GGNNCC 1 cut(s) 74
BmsI GCATC 1 cut(s) 188
BsaHI GRCGYC 1 cut(s) 90
Bsc4I CCNNNNNNNGG 1 cut(s) 175
Bse118I RCCGGY 1 cut(s) 65
BseGI GGATG 1 cut(s) 156
BseLI CCNNNNNNNGG 1 cut(s) 175
BshFI GGCC 2 cut(s) 225, 259
BshNI GGYRCC 1 cut(s) 72
BsiHKAI GWGCWC 1 cut(s) 233
BsiSI CCGG 2 cut(s) 66, 226
BslI CCNNNNNNNGG 1 cut(s) 175
BsnI GGCC 2 cut(s) 225, 259
Bsp1286I GDGCHC 1 cut(s) 233
Bsp143I GATC 1 cut(s) 6
BspACI CCGC 2 cut(s) 69, 267
BspANI GGCC 2 cut(s) 225, 259
BspLI GGNNCC 1 cut(s) 74
BspT107I GGYRCC 1 cut(s) 72
BsrFI RCCGGY 1 cut(s) 65
BssAI RCCGGY 1 cut(s) 65
BssMI GATC 1 cut(s) 6
BssNI GRCGYC 1 cut(s) 90
Bst4CI ACNGT 1 cut(s) 109
BstACI GRCGYC 1 cut(s) 90
BstC8I GCNNGC 1 cut(s) 67
BstF5I GGATG 1 cut(s) 156
BstKTI GATC 1 cut(s) 9
BstMBI GATC 1 cut(s) 6
BstMWI GCNNNNNNNGC 1 cut(s) 62
BstX2I RGATCY 1 cut(s) 6
BstYI RGATCY 1 cut(s) 6
BsuRI GGCC 2 cut(s) 225, 259
BtgZI GCGATG 1 cut(s) 172
BtsCI GGATG 1 cut(s) 156
Cac8I GCNNGC 1 cut(s) 67
Cfr10I RCCGGY 1 cut(s) 65
Cfr13I GGNCC 1 cut(s) 112
CspCI CAANNNNNGTGG 2 cut(s) 236, 271
CviAII CATG 1 cut(s) 278
CviJI RGCY 6 cut(s) 65, 159, 167, 185, 225, 259
CviKI_1 RGCY 6 cut(s) 65, 159, 167, 185, 225, 259
DpnI GATC 1 cut(s) 8
DpnII GATC 1 cut(s) 6
EaeI YGGCCR 1 cut(s) 223
Eco47I GGWCC 1 cut(s) 112
FaeI CATG 1 cut(s) 281
FaiI YATR 3 cut(s) 32, 60, 279
FatI CATG 1 cut(s) 277
FauI CCCGC 1 cut(s) 62
FokI GGATG 1 cut(s) 163
HaeIII GGCC 2 cut(s) 225, 259
HapII CCGG 2 cut(s) 66, 226
Hin1I GRCGYC 1 cut(s) 90
Hin1II CATG 1 cut(s) 281
HpaII CCGG 2 cut(s) 66, 226
Hpy188III TCNNGA 1 cut(s) 86
Hpy99I CGWCG 1 cut(s) 92
HpyCH4III ACNGT 1 cut(s) 109
HpyCH4IV ACGT 2 cut(s) 45, 90
HpyF10VI GCNNNNNNNGC 1 cut(s) 62
HpySE526I ACGT 2 cut(s) 45, 90
Hsp92I GRCGYC 1 cut(s) 90
Hsp92II CATG 1 cut(s) 281
KroI GCCGGC 1 cut(s) 65
KroNI GCCGGC 1 cut(s) 67
Kzo9I GATC 1 cut(s) 6
LmnI GCTCC 2 cut(s) 20, 228
LpnPI CCDG 3 cut(s) 79, 218, 239
LweI GCATC 1 cut(s) 188
MaeII ACGT 2 cut(s) 45, 90
MalI GATC 1 cut(s) 8
MboI GATC 1 cut(s) 6
MboII GAAGA 1 cut(s) 16
MflI RGATCY 1 cut(s) 6
MhlI GDGCHC 1 cut(s) 233
MluCI AATT 3 cut(s) 49, 94, 249
MmeI TCCRAC 1 cut(s) 139
MnlI CCTC 1 cut(s) 270
MroNI GCCGGC 1 cut(s) 65
MseI TTAA 1 cut(s) 283
MspI CCGG 2 cut(s) 66, 226
MwoI GCNNNNNNNGC 1 cut(s) 62
NaeI GCCGGC 1 cut(s) 67
NdeII GATC 1 cut(s) 6
NgoMIV GCCGGC 1 cut(s) 65
NlaIII CATG 1 cut(s) 281
NlaIV GGNNCC 1 cut(s) 74
PdiI GCCGGC 1 cut(s) 67
PflMI CCANNNNNTGG 1 cut(s) 175
PspN4I GGNNCC 1 cut(s) 74
PspPI GGNCC 1 cut(s) 112
PsuI RGATCY 1 cut(s) 6
SaqAI TTAA 1 cut(s) 283
Sau3AI GATC 1 cut(s) 6
Sau96I GGNCC 1 cut(s) 112
SduI GDGCHC 1 cut(s) 233
SetI ASST 3 cut(s) 48, 93, 130
SfaNI GCATC 1 cut(s) 188
SinI GGWCC 1 cut(s) 112
Sse9I AATT 3 cut(s) 49, 94, 249
SsiI CCGC 2 cut(s) 69, 267
TaaI ACNGT 1 cut(s) 109
TaiI ACGT 2 cut(s) 48, 93
TaqI TCGA 1 cut(s) 213
TasI AATT 3 cut(s) 49, 94, 249
Tru1I TTAA 1 cut(s) 283
Tru9I TTAA 1 cut(s) 283
TspDTI ATGAA 3 cut(s) 17, 19, 266
Van91I CCANNNNNTGG 1 cut(s) 175
VpaK11BI GGWCC 1 cut(s) 112
XapI RAATTY 1 cut(s) 249
ZraI GACGTC 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.