pycom17g16810

Protein of unknown function (DUF642)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0002157
Physical Location & Seq
Forward (+)
14807963 .. 14810859
2897 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g16810.21

Sequence Viewer

Length: 1890 bp
ATGGCTGCATCTAACGTCTCGTTAGACTGTCTACGTACCCTAAACAAGGATCAAGCCATTCGTAGTAATATACCTAATCAATTTAAAAGTGTCGATGGAACTATCAATAATATGTACGATAAAAGGGAACGATCCACTCACCTGGAGTCCACTGGCGCATGTGACCAATTATCGAATCACATCTCAGAACTCTTATCAATAGAATACAGTTCGAGACCCATTGACCACAAGTCAACCGTCGATCAAAGATCAACGGTAGGGTCTACACACTGTATAACTCGACCCAGAAGATCCGCATATCATAATAACATATTAAAATTTCATTACGATCCAACGGTCGGATCTCTGCCAATTGCCCAAAATAATCGGGAAGATCCATTACATATTATAATGTATCCAATTTTGGTGACGATCCAACGGTCGGATCGTCGATTCACATTTTTATCAAATAACGTATCTCAAGACATTCAACATATACGCGCCACCGTGGGTGGTGGTCGGCCACCCCGACTCGCCGGAAAATCCAACTATTTCCAAAAATTCTCAAACTTCACAGAAATGAAGATCTTAGTGAGTGGAGCAACTTTCATACCTACCACGAAGTCCAAAAGTGAACGGAAGATGGTCAATTTTGCCCACAAAGCCGGCGGGTGCCTAAAACTTCCCGGTGTCGATTCGTCTTCTACAGTGGTCCAACGGGGTCAAGGTTGGTTGGGTTTTGCTCCTGGGATGATGGGATACAAAGCTCAAGTGGTGGCATCGGCCATCGCTTTGCCGATTTGCCGAAAAATTGAAAAGATTGGTCGGAGCACTGTTGATTTTCGTCGACTTTTGTGGCCTCCAACCACCATATACCATGAGCTTCAAGATGGTGGTGGTGGCGTCGAAAAACTCCATCGGAGTTGGCCAAAATCGGCCTTGGAAAATGGGTGGTCACACAGGGTTAGGCACGGGTGCACGGGAAGCTTTCCCATTTTTATTGTTTTTTCTTTCTTTCTTTCTTTTCTGATTGGGTCTCACCCTTTCCCTCTAATTCTAATTGGTTCCTTGACCTTATACTTGATTGGTCCTTCCTTTTTGTGCCTAAAATCTGATTGGCCACTTTCCCACAAGAACTATACCGTTATAATCCGGACTCGCAAACGGTTTTCGCTTATGCATTCACACCAACGAGGTCAACGTAAGTCAAAATCTTTGCCTCTAGGGTATTTTCGTAAATTCACACACACGGGTAACACGTTGGTAAACTGTTTCGACCCATTAAGAAAAAAATTAGCCATATTAATATATAAAATATATTCTATATTAAACATATATTTTATTATGAGAGTTCCTTATTATCATTACTATGATAAATTTTACTTAACATGTTGTTACATACATACAAGTATAAAAAATGTGAAAGAATATATAAACACTCATGATTCATCATTATTTCTCTACGACCTCCAAACCCTCATGAATAATTATTGTAGAAGTGTAACAAATGTAAAAAATATATATACAATTACTCATAGTGACAAGCTTTACAACTTTCATGAAGGGGTCATTAACCATCGATTGTCATTTTACGCTTTATTCATCTTACTGAAATTCATTTTTAAAATTTTTCTTTTTTTGATATCACATTTCGATATTTACACAAGCCATATTTTCACTAACCGTAAAACTCTGAATATAATATCAACGATCCAAACCATTCATCTTCCTGCATCCTCTCATAGATCATGTTTTCAAAAAATAAAATCTGAAAAAATAAAATTTGATGAGAACAATGAAATGATTATAGTGGCGAATTTCAAAGTTAAGCTCTTCATGAAAGTTGTAAAGTTTGTCGTTACGAGCGTTTATATATATTTTCTATATTTTTTACACTTCTACATTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF642

Protein Analysis

630

Amino Acids

72.64

Weight (kDa)

9.84

Isoelectric Point (pI)

46.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 389, 1127
AccB1I GGYRCC 1 cut(s) 651
AccI GTMKAC 3 cut(s) 31, 263, 826
AccII CGCG 1 cut(s) 480
AccIII TCCGGA 1 cut(s) 1131
AciI CCGC 2 cut(s) 294, 648
AclWI GGATC 9 cut(s) 57, 126, 285, 323, 349, 368, 406, 432, 1684
AcoI YGGCCR 4 cut(s) 500, 762, 905, 1097
AcsI RAATTY 8 cut(s) 317, 539, 1217, 1355, 1592, 1605, 1760, 1795
AcyI GRCGYC 1 cut(s) 882
AfaI GTAC 2 cut(s) 37, 116
AfiI CCNNNNNNNGG 3 cut(s) 46, 338, 421
AflIII ACRYGT 2 cut(s) 1236, 1367
AgsI TTSAA 5 cut(s) 470, 794, 866, 1736, 1801
AhdI GACNNNNNGTC 1 cut(s) 229
AjnI CCWGG 2 cut(s) 141, 724
AluBI AGCT 5 cut(s) 746, 862, 966, 1525, 1810
AluI AGCT 5 cut(s) 746, 862, 966, 1525, 1810
Alw21I GWGCWC 2 cut(s) 812, 959
Alw26I GTCTC 3 cut(s) 22, 208, 1020
Alw44I GTGCAC 1 cut(s) 955
AlwI GGATC 9 cut(s) 57, 126, 285, 323, 349, 368, 406, 432, 1684
Aor13HI TCCGGA 1 cut(s) 1131
AoxI GGCC 6 cut(s) 500, 762, 836, 905, 915, 1097
ApaLI GTGCAC 1 cut(s) 955
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 8 cut(s) 317, 539, 1217, 1355, 1592, 1605, 1760, 1795
AseI ATTAAT 2 cut(s) 1283, 1884
AspLEI GCGC 2 cut(s) 158, 482
AspS9I GGNCC 2 cut(s) 691, 1067
AsuC2I CCSGG 1 cut(s) 666
AsuHPI GGTGA 3 cut(s) 131, 418, 1010
AvaII GGWCC 2 cut(s) 691, 1067
BaeGI GKGCMC 1 cut(s) 959
BalI TGGCCA 2 cut(s) 907, 1099
BanI GGYRCC 1 cut(s) 651
BarI GAAGNNNNNNTAC 2 cut(s) 363, 395
BbsI GAAGAC 1 cut(s) 672
Bbv12I GWGCWC 2 cut(s) 812, 959
BccI CCATC 7 cut(s) 89, 616, 727, 773, 863, 903, 1563
BciT130I CCWGG 2 cut(s) 143, 726
BciVI GTATCC 2 cut(s) 405, 731
BcnI CCSGG 1 cut(s) 666
BcoDI GTCTC 3 cut(s) 22, 208, 1020
BfaI CTAG 1 cut(s) 1202
BfmI CTRYAG 1 cut(s) 684
BfuI GTATCC 2 cut(s) 405, 731
BglII AGATCT 1 cut(s) 564
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 3 cut(s) 143, 666, 726
Bme18I GGWCC 2 cut(s) 691, 1067
BmeRI GACNNNNNGTC 1 cut(s) 229
BmgT120I GGNCC 2 cut(s) 691, 1067
BmiI GGNNCC 2 cut(s) 653, 1045
BmrFI CCNGG 3 cut(s) 143, 666, 726
BmsI GCATC 3 cut(s) 17, 767, 1721
BpiI GAAGAC 1 cut(s) 672
BpmI CTGGAG 1 cut(s) 164
BpuEI CTTGAG 2 cut(s) 444, 732
BpuMI CCSGG 1 cut(s) 666
Bsa29I ATCGAT 1 cut(s) 1558
BsaAI YACGTR 1 cut(s) 35
BsaHI GRCGYC 1 cut(s) 882
BsaI GGTCTC 2 cut(s) 208, 1020
BsaJI CCNNGG 3 cut(s) 486, 725, 918
BsaWI WCCGGW 1 cut(s) 1131
Bsc4I CCNNNNNNNGG 3 cut(s) 46, 338, 421
Bse118I RCCGGY 1 cut(s) 644
Bse1I ACTGG 1 cut(s) 157
BseAI TCCGGA 1 cut(s) 1131
BseBI CCWGG 2 cut(s) 143, 726
BseCI ATCGAT 1 cut(s) 1558
BseDI CCNNGG 3 cut(s) 486, 725, 918
BseGI GGATG 2 cut(s) 735, 1712
BseLI CCNNNNNNNGG 3 cut(s) 46, 338, 421
BseMII CTCAG 1 cut(s) 198
BseNI ACTGG 1 cut(s) 157
BseSI GKGCMC 1 cut(s) 959
Bsh1236I CGCG 1 cut(s) 480
Bsh1285I CGRYCG 2 cut(s) 339, 422
BshFI GGCC 6 cut(s) 502, 764, 838, 907, 917, 1099
BshNI GGYRCC 1 cut(s) 651
BshVI ATCGAT 1 cut(s) 1558
BsiEI CGRYCG 2 cut(s) 339, 422
BsiHKAI GWGCWC 2 cut(s) 812, 959
BsiSI CCGG 4 cut(s) 516, 645, 666, 1132
BslI CCNNNNNNNGG 3 cut(s) 46, 338, 421
BsmAI GTCTC 3 cut(s) 22, 208, 1020
BsmBI CGTCTC 1 cut(s) 22
BsmI GAATGC 1 cut(s) 1159
BsnI GGCC 6 cut(s) 502, 764, 838, 907, 917, 1099
Bso31I GGTCTC 2 cut(s) 208, 1020
Bsp1286I GDGCHC 2 cut(s) 812, 959
Bsp13I TCCGGA 1 cut(s) 1131
BspACI CCGC 2 cut(s) 294, 648
BspANI GGCC 6 cut(s) 502, 764, 838, 907, 917, 1099
BspCNI CTCAG 1 cut(s) 197
BspDI ATCGAT 1 cut(s) 1558
BspEI TCCGGA 1 cut(s) 1131
BspFNI CGCG 1 cut(s) 480
BspHI TCATGA 4 cut(s) 1420, 1458, 1537, 1815
BspLI GGNNCC 2 cut(s) 653, 1045
BspPI GGATC 9 cut(s) 57, 126, 285, 323, 349, 368, 406, 432, 1684
BspQI GCTCTTC 1 cut(s) 1817
BspT107I GGYRCC 1 cut(s) 651
BspTNI GGTCTC 2 cut(s) 208, 1020
BsrFI RCCGGY 1 cut(s) 644
BsrI ACTGG 1 cut(s) 157
BssAI RCCGGY 1 cut(s) 644
BssECI CCNNGG 3 cut(s) 486, 725, 918
BssNI GRCGYC 1 cut(s) 882
BssT1I CCWWGG 1 cut(s) 918
Bst2UI CCWGG 2 cut(s) 143, 726
Bst6I CTCTTC 1 cut(s) 1817
BstACI GRCGYC 1 cut(s) 882
BstBAI YACGTR 1 cut(s) 35
BstC8I GCNNGC 1 cut(s) 646
BstDEI CTNAG 2 cut(s) 184, 568
BstDSI CCRYGG 1 cut(s) 486
BstENI CCTNNNNNAGG 1 cut(s) 44
BstF5I GGATG 2 cut(s) 735, 1712
BstFNI CGCG 1 cut(s) 480
BstHHI GCGC 2 cut(s) 158, 482
BstMAI GTCTC 3 cut(s) 22, 208, 1020
BstMCI CGRYCG 2 cut(s) 339, 422
BstMWI GCNNNNNNNGC 2 cut(s) 641, 963
BstNI CCWGG 2 cut(s) 143, 726
BstNSI RCATGY 2 cut(s) 162, 1371
BstSCI CCNGG 3 cut(s) 141, 664, 724
BstSFI CTRYAG 1 cut(s) 684
BstSLI GKGCMC 1 cut(s) 959
BstSNI TACGTA 1 cut(s) 35
BstUI CGCG 1 cut(s) 480
BstV2I GAAGAC 1 cut(s) 672
BstX2I RGATCY 4 cut(s) 290, 341, 373, 564
BstXI CCANNNNNNTGG 1 cut(s) 142
BstYI RGATCY 4 cut(s) 290, 341, 373, 564
Bsu15I ATCGAT 1 cut(s) 1558
BsuI GTATCC 2 cut(s) 405, 731
BsuRI GGCC 6 cut(s) 502, 764, 838, 907, 917, 1099
BsuTUI ATCGAT 1 cut(s) 1558
BtgI CCRYGG 1 cut(s) 486
BtgZI GCGATG 1 cut(s) 751
BtsCI GGATG 2 cut(s) 735, 1712
BtsIMutI CAGTG 4 cut(s) 150, 268, 693, 810
Cac8I GCNNGC 1 cut(s) 646
CciI TCATGA 4 cut(s) 1420, 1458, 1537, 1815
CfoI GCGC 2 cut(s) 158, 482
Cfr10I RCCGGY 1 cut(s) 644
Cfr13I GGNCC 2 cut(s) 691, 1067
ClaI ATCGAT 1 cut(s) 1558
CseI GACGC 1 cut(s) 871
Csp6I GTAC 2 cut(s) 36, 115
CviAII CATG 8 cut(s) 159, 857, 1368, 1421, 1459, 1538, 1728, 1816
CviQI GTAC 2 cut(s) 36, 115
DdeI CTNAG 2 cut(s) 184, 568
DraI TTTAAA 2 cut(s) 85, 1603
DriI GACNNNNNGTC 1 cut(s) 229
EaeI YGGCCR 4 cut(s) 500, 762, 905, 1097
Eam1104I CTCTTC 1 cut(s) 1817
Eam1105I GACNNNNNGTC 1 cut(s) 229
EarI CTCTTC 1 cut(s) 1817
Eco105I TACGTA 1 cut(s) 35
Eco130I CCWWGG 1 cut(s) 918
Eco31I GGTCTC 2 cut(s) 208, 1020
Eco32I GATATC 1 cut(s) 1623
Eco47I GGWCC 2 cut(s) 691, 1067
EcoNI CCTNNNNNAGG 1 cut(s) 44
EcoRII CCWGG 2 cut(s) 141, 724
EcoRV GATATC 1 cut(s) 1623
EcoT14I CCWWGG 1 cut(s) 918
EcoT22I ATGCAT 1 cut(s) 1161
ErhI CCWWGG 1 cut(s) 918
Esp3I CGTCTC 1 cut(s) 22
FaeI CATG 8 cut(s) 162, 860, 1371, 1424, 1462, 1541, 1731, 1819
FatI CATG 8 cut(s) 158, 856, 1367, 1420, 1458, 1537, 1727, 1815
FauI CCCGC 1 cut(s) 641
FblI GTMKAC 3 cut(s) 31, 263, 826
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 2 cut(s) 742, 1699
Fsp4HI GCNGC 1 cut(s) 6
FspBI CTAG 1 cut(s) 1202
GlaI GCGC 2 cut(s) 157, 481
GluI GCNGC 1 cut(s) 6
GsuI CTGGAG 1 cut(s) 164
HaeIII GGCC 6 cut(s) 502, 764, 838, 907, 917, 1099
HapII CCGG 4 cut(s) 516, 645, 666, 1132
HgaI GACGC 1 cut(s) 871
HhaI GCGC 2 cut(s) 158, 482
Hin1I GRCGYC 1 cut(s) 882
Hin1II CATG 8 cut(s) 162, 860, 1371, 1424, 1462, 1541, 1731, 1819
Hin6I GCGC 2 cut(s) 156, 480
HinP1I GCGC 2 cut(s) 156, 480
HincII GTYRAC 3 cut(s) 234, 827, 1178
HindII GTYRAC 3 cut(s) 234, 827, 1178
HindIII AAGCTT 2 cut(s) 964, 1523
HinfI GANTC 7 cut(s) 146, 175, 432, 510, 674, 1135, 1424
HpaII CCGG 4 cut(s) 516, 645, 666, 1132
HphI GGTGA 3 cut(s) 131, 418, 1010
Hpy166II GTNNAC 9 cut(s) 32, 150, 234, 264, 614, 827, 957, 1178, 1246
Hpy188I TCNGA 9 cut(s) 187, 341, 424, 807, 900, 1008, 1093, 1674, 1750
Hpy188III TCNNGA 9 cut(s) 213, 368, 461, 866, 1132, 1421, 1459, 1538, 1816
Hpy8I GTNNAC 9 cut(s) 32, 150, 234, 264, 614, 827, 957, 1178, 1246
Hpy99I CGWCG 4 cut(s) 242, 432, 828, 887
HpyAV CCTTC 2 cut(s) 1080, 1535
HpyCH4IV ACGT 5 cut(s) 15, 34, 453, 1180, 1238
HpyCH4V TGCA 4 cut(s) 8, 957, 1159, 1712
HpyF10VI GCNNNNNNNGC 2 cut(s) 641, 963
HpyF3I CTNAG 2 cut(s) 184, 568
HpySE526I ACGT 5 cut(s) 15, 34, 453, 1180, 1238
Hsp92I GRCGYC 1 cut(s) 882
Hsp92II CATG 8 cut(s) 162, 860, 1371, 1424, 1462, 1541, 1731, 1819
HspAI GCGC 2 cut(s) 156, 480
Kpn2I TCCGGA 1 cut(s) 1131
KroI GCCGGC 1 cut(s) 644
KroNI GCCGGC 1 cut(s) 646
LguI GCTCTTC 1 cut(s) 1817
LmnI GCTCC 3 cut(s) 578, 727, 807
LweI GCATC 3 cut(s) 17, 767, 1721
MaeI CTAG 1 cut(s) 1202
MaeII ACGT 5 cut(s) 15, 34, 453, 1180, 1238
MaeIII GTNAC 8 cut(s) 161, 406, 933, 1232, 1373, 1480, 1517, 1837
MboII GAAGA 7 cut(s) 300, 383, 574, 631, 672, 1697, 1804
MfeI CAATTG 1 cut(s) 351
MflI RGATCY 4 cut(s) 290, 341, 373, 564
MhlI GDGCHC 2 cut(s) 812, 959
MlsI TGGCCA 2 cut(s) 907, 1099
MluNI TGGCCA 2 cut(s) 907, 1099
MlyI GAGTC 3 cut(s) 155, 504, 1129
MmeI TCCRAC 8 cut(s) 319, 356, 402, 439, 549, 718, 785, 866
MnlI CCTC 7 cut(s) 849, 1038, 1166, 1209, 1457, 1466, 1726
Mox20I TGGCCA 2 cut(s) 907, 1099
Mph1103I ATGCAT 1 cut(s) 1161
MroI TCCGGA 1 cut(s) 1131
MroNI GCCGGC 1 cut(s) 644
MscI TGGCCA 2 cut(s) 907, 1099
MslI CAYNNNNRTG 2 cut(s) 557, 1347
Msp20I TGGCCA 2 cut(s) 907, 1099
MspI CCGG 4 cut(s) 516, 645, 666, 1132
MspR9I CCNGG 3 cut(s) 143, 666, 726
MunI CAATTG 1 cut(s) 351
Mva1269I GAATGC 1 cut(s) 1159
MvaI CCWGG 2 cut(s) 143, 726
MvnI CGCG 1 cut(s) 480
MwoI GCNNNNNNNGC 2 cut(s) 641, 963
NaeI GCCGGC 1 cut(s) 646
NciI CCSGG 1 cut(s) 666
NgoMIV GCCGGC 1 cut(s) 644
NlaIII CATG 8 cut(s) 162, 860, 1371, 1424, 1462, 1541, 1731, 1819
NlaIV GGNNCC 2 cut(s) 653, 1045
NmuCI GTSAC 4 cut(s) 161, 406, 933, 1517
NsiI ATGCAT 1 cut(s) 1161
NspI RCATGY 2 cut(s) 162, 1371
PacI TTAATTAA 1 cut(s) 1888
PagI TCATGA 4 cut(s) 1420, 1458, 1537, 1815
PciI ACATGT 1 cut(s) 1367
PciSI GCTCTTC 1 cut(s) 1817
PcsI WCGNNNNNNNCGW 5 cut(s) 424, 505, 1177, 1235, 1842
PctI GAATGC 1 cut(s) 1159
PdiI GCCGGC 1 cut(s) 646
PfeI GAWTC 4 cut(s) 175, 432, 674, 1424
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 3 cut(s) 154, 504, 1129
PpsI GAGTC 3 cut(s) 154, 504, 1129
Ppu21I YACGTR 1 cut(s) 35
PscI ACATGT 1 cut(s) 1367
PshBI ATTAAT 2 cut(s) 1283, 1884
PsiI TTATAA 2 cut(s) 389, 1127
Psp6I CCWGG 2 cut(s) 141, 724
PspGI CCWGG 2 cut(s) 141, 724
PspN4I GGNNCC 2 cut(s) 653, 1045
PspPI GGNCC 2 cut(s) 691, 1067
PsuI RGATCY 4 cut(s) 290, 341, 373, 564
RsaI GTAC 2 cut(s) 37, 116
RsaNI GTAC 2 cut(s) 36, 115
RseI CAYNNNNRTG 2 cut(s) 557, 1347
SalI GTCGAC 1 cut(s) 825
SapI GCTCTTC 1 cut(s) 1817
SatI GCNGC 1 cut(s) 6
Sau96I GGNCC 2 cut(s) 691, 1067
SchI GAGTC 3 cut(s) 155, 504, 1129
ScrFI CCNGG 3 cut(s) 143, 666, 726
SduI GDGCHC 2 cut(s) 812, 959
SfaNI GCATC 3 cut(s) 17, 767, 1721
SfcI CTRYAG 1 cut(s) 684
SinI GGWCC 2 cut(s) 691, 1067
SmiMI CAYNNNNRTG 2 cut(s) 557, 1347
SmlI CTYRAG 2 cut(s) 459, 747
SmoI CTYRAG 2 cut(s) 459, 747
SnaBI TACGTA 1 cut(s) 35
SsiI CCGC 2 cut(s) 294, 648
SspMI CTAG 1 cut(s) 1202
StyD4I CCNGG 3 cut(s) 141, 664, 724
StyI CCWWGG 1 cut(s) 918
TaiI ACGT 5 cut(s) 18, 37, 456, 1183, 1241
TfiI GAWTC 4 cut(s) 175, 432, 674, 1424
TscAI CASTG 4 cut(s) 157, 275, 693, 817
TseFI GTSAC 4 cut(s) 161, 406, 933, 1517
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 4 cut(s) 161, 406, 933, 1517
TspGWI ACGGA 1 cut(s) 631
TspRI CASTG 4 cut(s) 157, 275, 693, 817
VneI GTGCAC 1 cut(s) 955
VpaK11BI GGWCC 2 cut(s) 691, 1067
VspI ATTAAT 2 cut(s) 1283, 1884
XagI CCTNNNNNAGG 1 cut(s) 44
XapI RAATTY 8 cut(s) 317, 539, 1217, 1355, 1592, 1605, 1760, 1795
XceI RCATGY 2 cut(s) 162, 1371
XmiI GTMKAC 3 cut(s) 31, 263, 826
XspI CTAG 1 cut(s) 1202
Zsp2I ATGCAT 1 cut(s) 1161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.