pycom09g20030

Protein of unknown function (DUF642)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0002157
Physical Location & Seq
Forward (+)
20850978 .. 20851325
348 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g20030.1

Sequence Viewer

Length: 348 bp
ATGACAAAACTGAATTCAAAACATTCGGCCACCCCGATTCGCCGGAAAATCCAACTATTTCCAAAAATTCTCAAACTTCACAGAAATGAAGATCGATCAGTGAGTGGAGCAACTTTCATACCTGCCACGAAGTCCAAAAGTGGACGGAAGATGGTCAATTTTGCCCACGAAACCGGCGGGTGCCTAAAACTTCCCGACGTCGATTTGTCGTCTACGGTGGTCCAACGGGGTCAAGGTTGGTTGGGTTTTGCTCCTGGGATGATGGGCTACAAAGCCCAAGTGGTGGCAGATGGCCATTGCTTTGCCGATTTGCCGGAAAATCGAGAAGGGTGGCCGGAGCACCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF642

Protein Analysis

116

Amino Acids

12.75

Weight (kDa)

9.8

Isoelectric Point (pI)

29.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 201
Acc36I ACCTGC 1 cut(s) 130
AccB1I GGYRCC 1 cut(s) 180
AccB7I CCANNNNNTGG 1 cut(s) 283
AccI GTMKAC 1 cut(s) 212
AciI CCGC 1 cut(s) 177
AcoI YGGCCR 3 cut(s) 27, 292, 332
AcsI RAATTY 2 cut(s) 13, 66
AcyI GRCGYC 1 cut(s) 198
AfiI CCNNNNNNNGG 1 cut(s) 283
AgsI TTSAA 1 cut(s) 18
AjnI CCWGG 1 cut(s) 253
Alw21I GWGCWC 1 cut(s) 342
AoxI GGCC 3 cut(s) 27, 292, 332
ApoI RAATTY 2 cut(s) 13, 66
AspS9I GGNCC 1 cut(s) 220
AvaII GGWCC 1 cut(s) 220
BalI TGGCCA 1 cut(s) 294
BanI GGYRCC 1 cut(s) 180
Bbv12I GWGCWC 1 cut(s) 342
BccI CCATC 3 cut(s) 145, 256, 284
BciT130I CCWGG 1 cut(s) 255
BfuAI ACCTGC 1 cut(s) 130
Bme1390I CCNGG 1 cut(s) 255
Bme18I GGWCC 1 cut(s) 220
BmgT120I GGNCC 1 cut(s) 220
BmiI GGNNCC 1 cut(s) 182
BmrFI CCNGG 1 cut(s) 255
Bsa29I ATCGAT 1 cut(s) 94
BsaHI GRCGYC 1 cut(s) 198
BsaJI CCNNGG 1 cut(s) 254
Bsc4I CCNNNNNNNGG 1 cut(s) 283
Bse118I RCCGGY 1 cut(s) 173
Bse3DI GCAATG 1 cut(s) 295
BseBI CCWGG 1 cut(s) 255
BseCI ATCGAT 1 cut(s) 94
BseDI CCNNGG 1 cut(s) 254
BseGI GGATG 1 cut(s) 264
BseLI CCNNNNNNNGG 1 cut(s) 283
BseMI GCAATG 1 cut(s) 295
BshFI GGCC 3 cut(s) 29, 294, 334
BshNI GGYRCC 1 cut(s) 180
BshVI ATCGAT 1 cut(s) 94
BsiHKAI GWGCWC 1 cut(s) 342
BsiSI CCGG 4 cut(s) 43, 174, 314, 335
BslI CCNNNNNNNGG 1 cut(s) 283
BsnI GGCC 3 cut(s) 29, 294, 334
Bsp1286I GDGCHC 1 cut(s) 342
Bsp143I GATC 2 cut(s) 91, 95
BspACI CCGC 1 cut(s) 177
BspANI GGCC 3 cut(s) 29, 294, 334
BspDI ATCGAT 1 cut(s) 94
BspLI GGNNCC 1 cut(s) 182
BspMI ACCTGC 1 cut(s) 130
BspT107I GGYRCC 1 cut(s) 180
BsrDI GCAATG 1 cut(s) 295
BsrFI RCCGGY 1 cut(s) 173
BssAI RCCGGY 1 cut(s) 173
BssECI CCNNGG 1 cut(s) 254
BssMI GATC 2 cut(s) 91, 95
BssNI GRCGYC 1 cut(s) 198
Bst2UI CCWGG 1 cut(s) 255
Bst4CI ACNGT 1 cut(s) 217
BstACI GRCGYC 1 cut(s) 198
BstF5I GGATG 1 cut(s) 264
BstKTI GATC 2 cut(s) 94, 98
BstMBI GATC 2 cut(s) 91, 95
BstNI CCWGG 1 cut(s) 255
BstSCI CCNGG 1 cut(s) 253
Bsu15I ATCGAT 1 cut(s) 94
BsuRI GGCC 3 cut(s) 29, 294, 334
BsuTUI ATCGAT 1 cut(s) 94
BtsCI GGATG 1 cut(s) 264
BtsIMutI CAGTG 1 cut(s) 105
BveI ACCTGC 1 cut(s) 130
Cfr10I RCCGGY 1 cut(s) 173
Cfr13I GGNCC 1 cut(s) 220
ClaI ATCGAT 1 cut(s) 94
CviJI RGCY 5 cut(s) 29, 267, 275, 294, 334
CviKI_1 RGCY 5 cut(s) 29, 267, 275, 294, 334
DpnI GATC 2 cut(s) 93, 97
DpnII GATC 2 cut(s) 91, 95
EaeI YGGCCR 3 cut(s) 27, 292, 332
Eco47I GGWCC 1 cut(s) 220
EcoRI GAATTC 1 cut(s) 13
EcoRII CCWGG 1 cut(s) 253
FaiI YATR 1 cut(s) 119
FauI CCCGC 1 cut(s) 170
FblI GTMKAC 1 cut(s) 212
FokI GGATG 1 cut(s) 271
HaeIII GGCC 3 cut(s) 29, 294, 334
HapII CCGG 4 cut(s) 43, 174, 314, 335
Hin1I GRCGYC 1 cut(s) 198
HinfI GANTC 1 cut(s) 37
HpaII CCGG 4 cut(s) 43, 174, 314, 335
Hpy166II GTNNAC 2 cut(s) 143, 213
Hpy188III TCNNGA 2 cut(s) 194, 323
Hpy8I GTNNAC 2 cut(s) 143, 213
Hpy99I CGWCG 2 cut(s) 200, 203
HpyAV CCTTC 1 cut(s) 320
HpyCH4III ACNGT 1 cut(s) 217
HpyCH4IV ACGT 1 cut(s) 198
HpySE526I ACGT 1 cut(s) 198
Hsp92I GRCGYC 1 cut(s) 198
Kzo9I GATC 2 cut(s) 91, 95
LmnI GCTCC 3 cut(s) 107, 256, 337
LpnPI CCDG 6 cut(s) 56, 135, 187, 240, 267, 327
MaeII ACGT 1 cut(s) 198
MalI GATC 2 cut(s) 93, 97
MboI GATC 2 cut(s) 91, 95
MboII GAAGA 2 cut(s) 101, 160
MhlI GDGCHC 1 cut(s) 342
MlsI TGGCCA 1 cut(s) 294
MluCI AATT 3 cut(s) 13, 66, 157
MluNI TGGCCA 1 cut(s) 294
MmeI TCCRAC 2 cut(s) 76, 247
Mox20I TGGCCA 1 cut(s) 294
MscI TGGCCA 1 cut(s) 294
MslI CAYNNNNRTG 1 cut(s) 84
Msp20I TGGCCA 1 cut(s) 294
MspI CCGG 4 cut(s) 43, 174, 314, 335
MspR9I CCNGG 1 cut(s) 255
MvaI CCWGG 1 cut(s) 255
NdeII GATC 2 cut(s) 91, 95
NlaIV GGNNCC 1 cut(s) 182
PcsI WCGNNNNNNNCGW 1 cut(s) 32
PfeI GAWTC 1 cut(s) 37
PflMI CCANNNNNTGG 1 cut(s) 283
Psp6I CCWGG 1 cut(s) 253
PspGI CCWGG 1 cut(s) 253
PspN4I GGNNCC 1 cut(s) 182
PspPI GGNCC 1 cut(s) 220
RseI CAYNNNNRTG 1 cut(s) 84
Sau3AI GATC 2 cut(s) 91, 95
Sau96I GGNCC 1 cut(s) 220
ScrFI CCNGG 1 cut(s) 255
SduI GDGCHC 1 cut(s) 342
SetI ASST 3 cut(s) 124, 201, 238
SinI GGWCC 1 cut(s) 220
SmiMI CAYNNNNRTG 1 cut(s) 84
Sse9I AATT 3 cut(s) 13, 66, 157
SsiI CCGC 1 cut(s) 177
StyD4I CCNGG 1 cut(s) 253
TaaI ACNGT 1 cut(s) 217
TaiI ACGT 1 cut(s) 201
TaqI TCGA 3 cut(s) 94, 201, 322
TasI AATT 3 cut(s) 13, 66, 157
TfiI GAWTC 1 cut(s) 37
TscAI CASTG 1 cut(s) 105
TspDTI ATGAA 2 cut(s) 102, 106
TspGWI ACGGA 1 cut(s) 160
TspRI CASTG 1 cut(s) 105
Van91I CCANNNNNTGG 1 cut(s) 283
VpaK11BI GGWCC 1 cut(s) 220
XapI RAATTY 2 cut(s) 13, 66
XmiI GTMKAC 1 cut(s) 212
ZraI GACGTC 1 cut(s) 199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.