pycom04g07720

Indole-3-acetic acid-amido synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0005203
Physical Location & Seq
Reverse (-)
8595593 .. 8596036
444 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g07720.1

Sequence Viewer

Length: 444 bp
ATGAATAGCAATAAGGTGGACTTTGCTCCTAAAGTGGCACCAAGGTCCATTCCTTTTGCTGTTGAGTCTAATTCGCCTGCTGAGAAAGAGAAGACTACTCACGTGGGCAATTGTGAGAAAACCACCAAGCCTGTTTCTAACGAGGCTGCTAAGATTTGTGTGCTCTTGAAACCAAGTTTGCTTGAAGGCATGGACACGTATACCAAGCTTGCTGATGGCGTTAAAGTGGTTGTTTACCCAAGTTCCTTTGTGAAGCATACGACCAAATATAGAAAAACTGCTCTGCTTGCTACGATGCAGAAAATGACGATTTTGGCAGCTGAGTCTATGCTCCTTAATCAAAAGGATACCAAGGCTGCCAAAGAGGTGGCAAAGATTAGGCAGTTGAAGCTTATTCCTCTGCCGAGAAGATCAAAAGGTTGGAGTCTGAGCTCGTTCCTTTAA

Protein Analysis

148

Amino Acids

16.22

Weight (kDa)

9.9

Isoelectric Point (pI)

28.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 37
AccI GTMKAC 1 cut(s) 200
AcvI CACGTG 1 cut(s) 103
AflIII ACRYGT 1 cut(s) 195
AgsI TTSAA 3 cut(s) 169, 185, 388
AluBI AGCT 4 cut(s) 208, 320, 391, 432
AluI AGCT 4 cut(s) 208, 320, 391, 432
Alw21I GWGCWC 2 cut(s) 165, 434
ApeKI GCWGC 3 cut(s) 146, 317, 356
AspS9I GGNCC 1 cut(s) 45
AvaII GGWCC 1 cut(s) 45
BanI GGYRCC 1 cut(s) 37
BanII GRGCYC 1 cut(s) 434
BbrPI CACGTG 1 cut(s) 103
BbsI GAAGAC 1 cut(s) 98
Bbv12I GWGCWC 2 cut(s) 165, 434
BbvI GCAGC 3 cut(s) 133, 329, 343
BccI CCATC 1 cut(s) 209
BciVI GTATCC 1 cut(s) 340
BfuI GTATCC 1 cut(s) 340
BisI GCNGC 3 cut(s) 147, 318, 357
BlsI GCNGC 3 cut(s) 148, 319, 358
Bme18I GGWCC 1 cut(s) 45
BmgT120I GGNCC 1 cut(s) 45
BmiI GGNNCC 1 cut(s) 39
BmsI GCATC 1 cut(s) 285
BpiI GAAGAC 1 cut(s) 98
BsaAI YACGTR 2 cut(s) 103, 198
BsaJI CCNNGG 2 cut(s) 41, 351
BseDI CCNNGG 2 cut(s) 41, 351
BseMII CTCAG 3 cut(s) 72, 312, 419
BseXI GCAGC 3 cut(s) 133, 329, 343
BshNI GGYRCC 1 cut(s) 37
BsiHKAI GWGCWC 2 cut(s) 165, 434
Bsp1286I GDGCHC 2 cut(s) 165, 434
Bsp143I GATC 1 cut(s) 410
BspCNI CTCAG 3 cut(s) 73, 313, 420
BspLI GGNNCC 1 cut(s) 39
BspT107I GGYRCC 1 cut(s) 37
BssECI CCNNGG 2 cut(s) 41, 351
BssMI GATC 1 cut(s) 410
BssNAI GTATAC 1 cut(s) 201
BssT1I CCWWGG 2 cut(s) 41, 351
Bst1107I GTATAC 1 cut(s) 201
BstBAI YACGTR 2 cut(s) 103, 198
BstC8I GCNNGC 3 cut(s) 78, 210, 288
BstDEI CTNAG 4 cut(s) 81, 150, 321, 428
BstKTI GATC 1 cut(s) 413
BstMBI GATC 1 cut(s) 410
BstMWI GCNNNNNNNGC 2 cut(s) 287, 388
BstV1I GCAGC 3 cut(s) 133, 329, 343
BstV2I GAAGAC 1 cut(s) 98
BstXI CCANNNNNNTGG 1 cut(s) 367
BstZ17I GTATAC 1 cut(s) 201
BsuI GTATCC 1 cut(s) 340
Cac8I GCNNGC 3 cut(s) 78, 210, 288
Cfr13I GGNCC 1 cut(s) 45
CviAII CATG 1 cut(s) 190
CviJI RGCY 7 cut(s) 130, 146, 208, 320, 356, 391, 432
CviKI_1 RGCY 7 cut(s) 130, 146, 208, 320, 356, 391, 432
DdeI CTNAG 4 cut(s) 81, 150, 321, 428
DpnI GATC 1 cut(s) 412
DpnII GATC 1 cut(s) 410
Ecl136II GAGCTC 1 cut(s) 432
Eco130I CCWWGG 2 cut(s) 41, 351
Eco24I GRGCYC 1 cut(s) 434
Eco47I GGWCC 1 cut(s) 45
Eco53kI GAGCTC 1 cut(s) 432
Eco72I CACGTG 1 cut(s) 103
EcoICRI GAGCTC 1 cut(s) 432
EcoT14I CCWWGG 2 cut(s) 41, 351
EcoT38I GRGCYC 1 cut(s) 434
ErhI CCWWGG 2 cut(s) 41, 351
FaeI CATG 1 cut(s) 193
FaiI YATR 5 cut(s) 191, 201, 258, 270, 329
FalI AAGNNNNNCTT 1 cut(s) 37
FatI CATG 1 cut(s) 189
FblI GTMKAC 1 cut(s) 200
Fnu4HI GCNGC 3 cut(s) 147, 318, 357
FriOI GRGCYC 1 cut(s) 434
Fsp4HI GCNGC 3 cut(s) 147, 318, 357
GluI GCNGC 3 cut(s) 147, 318, 357
Hin1II CATG 1 cut(s) 193
HindIII AAGCTT 2 cut(s) 206, 389
HinfI GANTC 3 cut(s) 65, 323, 424
Hpy166II GTNNAC 3 cut(s) 19, 201, 235
Hpy188I TCNGA 1 cut(s) 429
Hpy188III TCNNGA 1 cut(s) 166
Hpy8I GTNNAC 3 cut(s) 19, 201, 235
HpyAV CCTTC 1 cut(s) 179
HpyCH4IV ACGT 2 cut(s) 102, 197
HpyCH4V TGCA 1 cut(s) 298
HpyF10VI GCNNNNNNNGC 2 cut(s) 287, 388
HpyF3I CTNAG 4 cut(s) 81, 150, 321, 428
HpySE526I ACGT 2 cut(s) 102, 197
Hsp92II CATG 1 cut(s) 193
Kzo9I GATC 1 cut(s) 410
LmnI GCTCC 2 cut(s) 31, 336
LpnPI CCDG 2 cut(s) 90, 144
Lsp1109I GCAGC 3 cut(s) 133, 329, 343
LweI GCATC 1 cut(s) 285
MaeII ACGT 2 cut(s) 102, 197
MalI GATC 1 cut(s) 412
MboI GATC 1 cut(s) 410
MboII GAAGA 2 cut(s) 103, 420
MfeI CAATTG 1 cut(s) 109
MhlI GDGCHC 2 cut(s) 165, 434
MluCI AATT 2 cut(s) 70, 109
MlyI GAGTC 3 cut(s) 74, 332, 433
MmeI TCCRAC 1 cut(s) 401
MnlI CCTC 3 cut(s) 136, 358, 408
MseI TTAA 3 cut(s) 222, 336, 442
MspA1I CMGCKG 1 cut(s) 320
MunI CAATTG 1 cut(s) 109
MwoI GCNNNNNNNGC 2 cut(s) 287, 388
NdeII GATC 1 cut(s) 410
NlaIII CATG 1 cut(s) 193
NlaIV GGNNCC 1 cut(s) 39
NmeAIII GCCGAG 1 cut(s) 429
PkrI GCNGC 3 cut(s) 148, 319, 358
PleI GAGTC 3 cut(s) 73, 331, 432
PmaCI CACGTG 1 cut(s) 103
PmlI CACGTG 1 cut(s) 103
PpsI GAGTC 3 cut(s) 73, 331, 432
Ppu21I YACGTR 2 cut(s) 103, 198
Psp124BI GAGCTC 1 cut(s) 434
PspCI CACGTG 1 cut(s) 103
PspN4I GGNNCC 1 cut(s) 39
PspPI GGNCC 1 cut(s) 45
PvuII CAGCTG 1 cut(s) 320
SacI GAGCTC 1 cut(s) 434
SaqAI TTAA 3 cut(s) 222, 336, 442
SatI GCNGC 3 cut(s) 147, 318, 357
Sau3AI GATC 1 cut(s) 410
Sau96I GGNCC 1 cut(s) 45
SchI GAGTC 3 cut(s) 74, 332, 433
SduI GDGCHC 2 cut(s) 165, 434
SfaNI GCATC 1 cut(s) 285
SinI GGWCC 1 cut(s) 45
Sse9I AATT 2 cut(s) 70, 109
SstI GAGCTC 1 cut(s) 434
StyI CCWWGG 2 cut(s) 41, 351
TaiI ACGT 2 cut(s) 105, 200
TasI AATT 2 cut(s) 70, 109
Tru1I TTAA 3 cut(s) 222, 336, 442
Tru9I TTAA 3 cut(s) 222, 336, 442
TseI GCWGC 3 cut(s) 146, 317, 356
TspDTI ATGAA 1 cut(s) 17
VpaK11BI GGWCC 1 cut(s) 45
XmiI GTMKAC 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.