pycom06g08870

Indole-3-acetic acid-amido synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0005203
Physical Location & Seq
Reverse (-)
14115446 .. 14115811
366 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g08870.1

Sequence Viewer

Length: 366 bp
ATGCACCATGAGTACTTCTTCAGGGCATCTCTAATAAAAGATGCAAATGCCTTAACATGTGTATTTTGCTTTTGTGCTTCTAGAGGTTTTCTTGAGATTTGTGTGCTGCATAAAGCTAACCTGCTCGAAGACCTTGTAGTGTGTGCCAAGTTTTTTGAGAACGTTGAAGAGGTGGTTCACCTGGGTTTCTTTGCAAATCATCTTGCTGAAGAGAAAAGAGTCCCCCTCTTTGCCACCATGCAGAAGACTTTGATTTTAGCTACTGAGACGATGCATCTTGACTATGATGTAGTTCATGTTGTGAAACAGACTGAGGCAGTGATGGAATCTAAAGCTCGCTCTGCTTCAAAAATGTTGAAGAGTTGA

Protein Analysis

122

Amino Acids

13.8

Weight (kDa)

6.36

Isoelectric Point (pI)

48.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 129
AclI AACGTT 1 cut(s) 162
AcuI CTGAAG 2 cut(s) 4, 228
AfaI GTAC 1 cut(s) 14
AflIII ACRYGT 1 cut(s) 56
AgsI TTSAA 3 cut(s) 167, 348, 358
AjnI CCWGG 1 cut(s) 180
AluBI AGCT 3 cut(s) 116, 260, 335
AluI AGCT 3 cut(s) 116, 260, 335
Alw26I GTCTC 1 cut(s) 260
ApeKI GCWGC 1 cut(s) 106
AsuHPI GGTGA 1 cut(s) 170
BbsI GAAGAC 2 cut(s) 135, 251
BbvI GCAGC 1 cut(s) 93
BccI CCATC 1 cut(s) 316
BciT130I CCWGG 1 cut(s) 182
BcoDI GTCTC 1 cut(s) 260
BfaI CTAG 1 cut(s) 81
BfuAI ACCTGC 1 cut(s) 129
BisI GCNGC 1 cut(s) 107
BlsI GCNGC 1 cut(s) 108
BmcAI AGTACT 1 cut(s) 14
Bme1390I CCNGG 1 cut(s) 182
BmrFI CCNGG 1 cut(s) 182
BmsI GCATC 4 cut(s) 31, 35, 261, 283
BpiI GAAGAC 2 cut(s) 135, 251
BplI GAGNNNNNCTC 2 cut(s) 210, 242
BpuEI CTTGAG 1 cut(s) 113
BsaJI CCNNGG 1 cut(s) 181
BseBI CCWGG 1 cut(s) 182
BseDI CCNNGG 1 cut(s) 181
BseMII CTCAG 2 cut(s) 255, 303
BseXI GCAGC 1 cut(s) 93
BslFI GGGAC 1 cut(s) 206
BsmAI GTCTC 1 cut(s) 260
BsmBI CGTCTC 1 cut(s) 260
BsmFI GGGAC 1 cut(s) 206
BspCNI CTCAG 2 cut(s) 256, 304
BspMI ACCTGC 1 cut(s) 129
BssECI CCNNGG 1 cut(s) 181
Bst2UI CCWGG 1 cut(s) 182
Bst6I CTCTTC 3 cut(s) 162, 204, 353
BstC8I GCNNGC 1 cut(s) 337
BstDEI CTNAG 2 cut(s) 264, 312
BstMAI GTCTC 1 cut(s) 260
BstMWI GCNNNNNNNGC 1 cut(s) 341
BstNI CCWGG 1 cut(s) 182
BstNSI RCATGY 1 cut(s) 60
BstSCI CCNGG 1 cut(s) 180
BstV1I GCAGC 1 cut(s) 93
BstV2I GAAGAC 2 cut(s) 135, 251
BtsI GCAGTG 1 cut(s) 324
BtsIMutI CAGTG 1 cut(s) 324
BveI ACCTGC 1 cut(s) 129
Cac8I GCNNGC 1 cut(s) 337
Csp6I GTAC 1 cut(s) 13
CviAII CATG 4 cut(s) 8, 57, 238, 296
CviJI RGCY 3 cut(s) 116, 260, 335
CviKI_1 RGCY 3 cut(s) 116, 260, 335
CviQI GTAC 1 cut(s) 13
DdeI CTNAG 2 cut(s) 264, 312
Eam1104I CTCTTC 3 cut(s) 162, 204, 353
EarI CTCTTC 3 cut(s) 162, 204, 353
Eco57I CTGAAG 2 cut(s) 4, 228
EcoRII CCWGG 1 cut(s) 180
EcoT22I ATGCAT 1 cut(s) 276
Esp3I CGTCTC 1 cut(s) 260
FaeI CATG 4 cut(s) 11, 60, 241, 299
FaiI YATR 6 cut(s) 9, 58, 111, 239, 285, 297
FaqI GGGAC 1 cut(s) 206
FatI CATG 4 cut(s) 7, 56, 237, 295
Fnu4HI GCNGC 1 cut(s) 107
Fsp4HI GCNGC 1 cut(s) 107
FspBI CTAG 1 cut(s) 81
GluI GCNGC 1 cut(s) 107
Hin1II CATG 4 cut(s) 11, 60, 241, 299
HinfI GANTC 2 cut(s) 219, 326
HphI GGTGA 1 cut(s) 170
Hpy166II GTNNAC 1 cut(s) 178
Hpy188III TCNNGA 3 cut(s) 81, 92, 278
Hpy8I GTNNAC 1 cut(s) 178
HpyCH4IV ACGT 1 cut(s) 162
HpyCH4V TGCA 6 cut(s) 4, 44, 109, 194, 241, 274
HpyF10VI GCNNNNNNNGC 1 cut(s) 341
HpyF3I CTNAG 2 cut(s) 264, 312
HpySE526I ACGT 1 cut(s) 162
Hsp92II CATG 4 cut(s) 11, 60, 241, 299
LpnPI CCDG 4 cut(s) 7, 134, 167, 194
Lsp1109I GCAGC 1 cut(s) 93
LweI GCATC 4 cut(s) 31, 35, 261, 283
MaeI CTAG 1 cut(s) 81
MaeII ACGT 1 cut(s) 162
MboII GAAGA 5 cut(s) 10, 140, 179, 221, 256
MlyI GAGTC 1 cut(s) 228
MnlI CCTC 4 cut(s) 77, 163, 236, 307
Mph1103I ATGCAT 1 cut(s) 276
MseI TTAA 1 cut(s) 53
MspR9I CCNGG 1 cut(s) 182
MvaI CCWGG 1 cut(s) 182
MwoI GCNNNNNNNGC 1 cut(s) 341
NlaIII CATG 4 cut(s) 11, 60, 241, 299
NsiI ATGCAT 1 cut(s) 276
NspI RCATGY 1 cut(s) 60
PciI ACATGT 1 cut(s) 56
PfeI GAWTC 1 cut(s) 326
PkrI GCNGC 1 cut(s) 108
PleI GAGTC 1 cut(s) 227
PpsI GAGTC 1 cut(s) 227
PscI ACATGT 1 cut(s) 56
Psp1406I AACGTT 1 cut(s) 162
Psp6I CCWGG 1 cut(s) 180
PspGI CCWGG 1 cut(s) 180
RsaI GTAC 1 cut(s) 14
RsaNI GTAC 1 cut(s) 13
SaqAI TTAA 1 cut(s) 53
SatI GCNGC 1 cut(s) 107
ScaI AGTACT 1 cut(s) 14
SchI GAGTC 1 cut(s) 228
ScrFI CCNGG 1 cut(s) 182
SetI ASST 9 cut(s) 88, 118, 123, 135, 165, 174, 183, 262, 337
SfaNI GCATC 4 cut(s) 31, 35, 261, 283
SmlI CTYRAG 1 cut(s) 92
SmoI CTYRAG 1 cut(s) 92
SspMI CTAG 1 cut(s) 81
StyD4I CCNGG 1 cut(s) 180
TaiI ACGT 1 cut(s) 165
TaqI TCGA 1 cut(s) 126
TatI WGTACW 1 cut(s) 12
TfiI GAWTC 1 cut(s) 326
Tru1I TTAA 1 cut(s) 53
Tru9I TTAA 1 cut(s) 53
TscAI CASTG 1 cut(s) 324
TseI GCWGC 1 cut(s) 106
TspDTI ATGAA 1 cut(s) 284
TspRI CASTG 1 cut(s) 324
XbaI TCTAGA 1 cut(s) 80
XceI RCATGY 1 cut(s) 60
XspI CTAG 1 cut(s) 81
ZrmI AGTACT 1 cut(s) 14
Zsp2I ATGCAT 1 cut(s) 276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.