pycom14g06700

exonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0005203
Physical Location & Seq
Reverse (-)
6691941 .. 6693009
1069 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g06700.2

Sequence Viewer

Length: 564 bp
ATGTCGGTTGAGAAAAAACTGAAGACTTCCTCCGTTGCTCGTAAGGGTGCACCTGCTATGAAAAAGCCTGTGATTTATATGAATTCTTCCTATGGGAAGAAAAATGAGGCTGCTAGATTTGAGCCTGTGATGCCTACCATGTCAAGAATGGCTAGTACGATTACGAATAGGATTGCTCAATATAGAGGTCCTGTCATGCCCCCAGTCCTGAAGTCTGTACCCAGACGTTTGTTGGGAGCCAAGTCTAGTTCACATTTGGAGAGGCTTGTTATTATGAAAAGCGATAAGCACATCCGTGTTGGTCTGCTCGAGGAGAACGAGCAACTGAAGGGTGAAAAGGCTGGGCTCGAGGCTTCGATTGTTCAAAGTCATGCCAATTTCTACAAGCTAGTTTATGTAGATCATCTCTTTGGGCGGTCGTCTGACTTTGAGGTTTTTGGTGAAGACTTCGAGATCTTCTCTATTTCTCCGGGAAACTTGTTCGCCTTTACTTTTGAGTCTTCCATTAGTGAAGTAGTTAGAGAAGTCAGTGCCCAGGCTGGGCAGCTGAGGGTGAAGCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
EloA-BP1

Protein Analysis

188

Amino Acids

20.91

Weight (kDa)

9.87

Isoelectric Point (pI)

49.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 61
Acc36I ACCTGC 1 cut(s) 61
AciI CCGC 1 cut(s) 415
AcsI RAATTY 1 cut(s) 82
AcuI CTGAAG 3 cut(s) 41, 230, 347
AfaI GTAC 2 cut(s) 157, 219
AfeI AGCGCT 1 cut(s) 560
AfiI CCNNNNNNNGG 1 cut(s) 540
AgsI TTSAA 1 cut(s) 365
AjnI CCWGG 1 cut(s) 534
AleI CACNNNNGTG 1 cut(s) 294
AluBI AGCT 2 cut(s) 388, 547
AluI AGCT 2 cut(s) 388, 547
Alw21I GWGCWC 1 cut(s) 52
Alw44I GTGCAC 1 cut(s) 48
Ama87I CYCGRG 2 cut(s) 308, 347
Aor51HI AGCGCT 1 cut(s) 560
ApaLI GTGCAC 1 cut(s) 48
ApeKI GCWGC 2 cut(s) 110, 544
ApoI RAATTY 1 cut(s) 82
AspLEI GCGC 1 cut(s) 561
AspS9I GGNCC 1 cut(s) 188
AsuC2I CCSGG 1 cut(s) 471
AsuHPI GGTGA 2 cut(s) 344, 452
AvaI CYCGRG 2 cut(s) 308, 347
AvaII GGWCC 1 cut(s) 188
BaeGI GKGCMC 2 cut(s) 52, 535
BanII GRGCYC 1 cut(s) 348
BbsI GAAGAC 3 cut(s) 29, 450, 492
Bbv12I GWGCWC 1 cut(s) 52
BbvCI CCTCAGC 1 cut(s) 548
BbvI GCAGC 2 cut(s) 97, 556
BciT130I CCWGG 1 cut(s) 536
BcnI CCSGG 1 cut(s) 471
BfaI CTAG 4 cut(s) 114, 153, 246, 389
BfoI RGCGCY 1 cut(s) 562
BfuAI ACCTGC 1 cut(s) 61
BglII AGATCT 1 cut(s) 453
BisI GCNGC 2 cut(s) 111, 545
BlsI GCNGC 2 cut(s) 112, 546
Bme1390I CCNGG 2 cut(s) 471, 536
Bme18I GGWCC 1 cut(s) 188
BmeT110I CYCGRG 2 cut(s) 308, 347
BmgT120I GGNCC 1 cut(s) 188
BmiI GGNNCC 1 cut(s) 238
BmrFI CCNGG 2 cut(s) 471, 536
BmrI ACTGGG 1 cut(s) 197
BmsI GCATC 1 cut(s) 120
BmuI ACTGGG 1 cut(s) 197
BpiI GAAGAC 3 cut(s) 29, 450, 492
BplI GAGNNNNNCTC 2 cut(s) 443, 475
Bpu10I CCTNAGC 1 cut(s) 548
BpuMI CCSGG 1 cut(s) 471
BsaJI CCNNGG 1 cut(s) 534
Bsc4I CCNNNNNNNGG 1 cut(s) 540
Bse1I ACTGG 1 cut(s) 203
BseBI CCWGG 1 cut(s) 536
BseDI CCNNGG 1 cut(s) 534
BseGI GGATG 1 cut(s) 291
BseLI CCNNNNNNNGG 1 cut(s) 540
BseMII CTCAG 1 cut(s) 539
BseNI ACTGG 1 cut(s) 203
BseRI GAGGAG 1 cut(s) 326
BseSI GKGCMC 2 cut(s) 52, 535
BseXI GCAGC 2 cut(s) 97, 556
BseYI CCCAGC 2 cut(s) 341, 539
Bsh1285I CGRYCG 1 cut(s) 419
BsiEI CGRYCG 1 cut(s) 419
BsiHKAI GWGCWC 1 cut(s) 52
BsiHKCI CYCGRG 2 cut(s) 308, 347
BsiSI CCGG 1 cut(s) 470
BslI CCNNNNNNNGG 1 cut(s) 540
BsoBI CYCGRG 2 cut(s) 308, 347
Bsp1286I GDGCHC 3 cut(s) 52, 348, 535
Bsp143I GATC 2 cut(s) 400, 453
BspACI CCGC 1 cut(s) 415
BspCNI CTCAG 1 cut(s) 540
BspLI GGNNCC 1 cut(s) 238
BspMI ACCTGC 1 cut(s) 61
BsrI ACTGG 1 cut(s) 203
BssECI CCNNGG 1 cut(s) 534
BssMI GATC 2 cut(s) 400, 453
Bst2UI CCWGG 1 cut(s) 536
BstDEI CTNAG 1 cut(s) 548
BstF5I GGATG 1 cut(s) 291
BstH2I RGCGCY 1 cut(s) 562
BstHHI GCGC 1 cut(s) 561
BstKTI GATC 2 cut(s) 403, 456
BstMBI GATC 2 cut(s) 400, 453
BstMCI CGRYCG 1 cut(s) 419
BstMWI GCNNNNNNNGC 1 cut(s) 130
BstNI CCWGG 1 cut(s) 536
BstSCI CCNGG 2 cut(s) 469, 534
BstSLI GKGCMC 2 cut(s) 52, 535
BstV1I GCAGC 2 cut(s) 97, 556
BstV2I GAAGAC 3 cut(s) 29, 450, 492
BstX2I RGATCY 1 cut(s) 453
BstYI RGATCY 1 cut(s) 453
BtsCI GGATG 1 cut(s) 291
BtsIMutI CAGTG 1 cut(s) 535
BveI ACCTGC 1 cut(s) 61
CfoI GCGC 1 cut(s) 561
Cfr13I GGNCC 1 cut(s) 188
Csp6I GTAC 2 cut(s) 156, 218
CviAII CATG 3 cut(s) 139, 196, 371
CviQI GTAC 2 cut(s) 156, 218
DdeI CTNAG 1 cut(s) 548
DpnI GATC 2 cut(s) 402, 455
DpnII GATC 2 cut(s) 400, 453
Eco24I GRGCYC 1 cut(s) 348
Eco47I GGWCC 1 cut(s) 188
Eco47III AGCGCT 1 cut(s) 560
Eco57I CTGAAG 3 cut(s) 41, 230, 347
Eco88I CYCGRG 2 cut(s) 308, 347
EcoO109I RGGNCCY 1 cut(s) 188
EcoRI GAATTC 1 cut(s) 82
EcoRII CCWGG 1 cut(s) 534
EcoT38I GRGCYC 1 cut(s) 348
FaeI CATG 3 cut(s) 142, 199, 374
FatI CATG 3 cut(s) 138, 195, 370
Fnu4HI GCNGC 2 cut(s) 111, 545
FokI GGATG 1 cut(s) 278
FriOI GRGCYC 1 cut(s) 348
Fsp4HI GCNGC 2 cut(s) 111, 545
FspBI CTAG 4 cut(s) 114, 153, 246, 389
GlaI GCGC 1 cut(s) 560
GluI GCNGC 2 cut(s) 111, 545
GsaI CCCAGC 2 cut(s) 345, 543
HaeII RGCGCY 1 cut(s) 562
HapII CCGG 1 cut(s) 470
HhaI GCGC 1 cut(s) 561
Hin1II CATG 3 cut(s) 142, 199, 374
Hin6I GCGC 1 cut(s) 559
HinP1I GCGC 1 cut(s) 559
HinfI GANTC 1 cut(s) 497
HpaII CCGG 1 cut(s) 470
HphI GGTGA 2 cut(s) 344, 452
Hpy166II GTNNAC 2 cut(s) 50, 251
Hpy188I TCNGA 1 cut(s) 424
Hpy188III TCNNGA 3 cut(s) 144, 208, 451
Hpy8I GTNNAC 2 cut(s) 50, 251
HpyAV CCTTC 1 cut(s) 322
HpyCH4IV ACGT 1 cut(s) 226
HpyCH4V TGCA 1 cut(s) 50
HpyF10VI GCNNNNNNNGC 1 cut(s) 130
HpyF3I CTNAG 1 cut(s) 548
HpySE526I ACGT 1 cut(s) 226
Hsp92II CATG 3 cut(s) 142, 199, 374
HspAI GCGC 1 cut(s) 559
Kzo9I GATC 2 cut(s) 400, 453
LmnI GCTCC 1 cut(s) 236
Lsp1109I GCAGC 2 cut(s) 97, 556
LweI GCATC 1 cut(s) 120
MaeI CTAG 4 cut(s) 114, 153, 246, 389
MaeII ACGT 1 cut(s) 226
MalI GATC 2 cut(s) 402, 455
MboI GATC 2 cut(s) 400, 453
MboII GAAGA 6 cut(s) 34, 78, 109, 448, 455, 492
MflI RGATCY 1 cut(s) 453
MhlI GDGCHC 3 cut(s) 52, 348, 535
MluCI AATT 2 cut(s) 82, 376
MlyI GAGTC 1 cut(s) 506
MnlI CCTC 8 cut(s) 40, 100, 179, 255, 304, 343, 424, 543
MslI CAYNNNNRTG 1 cut(s) 294
MspA1I CMGCKG 1 cut(s) 547
MspI CCGG 1 cut(s) 470
MspR9I CCNGG 2 cut(s) 471, 536
MvaI CCWGG 1 cut(s) 536
MwoI GCNNNNNNNGC 1 cut(s) 130
NciI CCSGG 1 cut(s) 471
NdeII GATC 2 cut(s) 400, 453
NlaIII CATG 3 cut(s) 142, 199, 374
NlaIV GGNNCC 1 cut(s) 238
OliI CACNNNNGTG 1 cut(s) 294
PaeR7I CTCGAG 2 cut(s) 308, 347
PaqCI CACCTGC 1 cut(s) 61
PcsI WCGNNNNNNNCGW 1 cut(s) 315
PfoI TCCNGGA 1 cut(s) 469
PkrI GCNGC 2 cut(s) 112, 546
PleI GAGTC 1 cut(s) 505
PpsI GAGTC 1 cut(s) 505
PpuMI RGGWCCY 1 cut(s) 188
Psp5II RGGWCCY 1 cut(s) 188
Psp6I CCWGG 1 cut(s) 534
PspFI CCCAGC 2 cut(s) 341, 539
PspGI CCWGG 1 cut(s) 534
PspN4I GGNNCC 1 cut(s) 238
PspPI GGNCC 1 cut(s) 188
PspPPI RGGWCCY 1 cut(s) 188
PspXI VCTCGAGB 2 cut(s) 308, 347
PsuI RGATCY 1 cut(s) 453
PvuII CAGCTG 1 cut(s) 547
RsaI GTAC 2 cut(s) 157, 219
RsaNI GTAC 2 cut(s) 156, 218
RseI CAYNNNNRTG 1 cut(s) 294
SatI GCNGC 2 cut(s) 111, 545
Sau3AI GATC 2 cut(s) 400, 453
Sau96I GGNCC 1 cut(s) 188
SchI GAGTC 1 cut(s) 506
ScrFI CCNGG 2 cut(s) 471, 536
SduI GDGCHC 3 cut(s) 52, 348, 535
SetI ASST 6 cut(s) 55, 190, 229, 390, 435, 549
SfaNI GCATC 1 cut(s) 120
Sfr274I CTCGAG 2 cut(s) 308, 347
SinI GGWCC 1 cut(s) 188
SlaI CTCGAG 2 cut(s) 308, 347
SmiMI CAYNNNNRTG 1 cut(s) 294
SmlI CTYRAG 2 cut(s) 308, 347
SmoI CTYRAG 2 cut(s) 308, 347
Sse9I AATT 2 cut(s) 82, 376
SsiI CCGC 1 cut(s) 415
SspMI CTAG 4 cut(s) 114, 153, 246, 389
StyD4I CCNGG 2 cut(s) 469, 534
TaiI ACGT 1 cut(s) 229
TaqI TCGA 4 cut(s) 309, 348, 356, 450
TasI AATT 2 cut(s) 82, 376
TscAI CASTG 1 cut(s) 535
TseI GCWGC 2 cut(s) 110, 544
TspDTI ATGAA 3 cut(s) 74, 95, 290
TspGWI ACGGA 2 cut(s) 22, 284
TspRI CASTG 1 cut(s) 535
VneI GTGCAC 1 cut(s) 48
VpaK11BI GGWCC 1 cut(s) 188
XapI RAATTY 1 cut(s) 82
XcmI CCANNNNNNNNNTGG 2 cut(s) 145, 229
XhoI CTCGAG 2 cut(s) 308, 347
XspI CTAG 4 cut(s) 114, 153, 246, 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.