pycom11g13350

exonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0005203
Physical Location & Seq
Forward (+)
12629283 .. 12629768
486 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g13350.1

Sequence Viewer

Length: 486 bp
ATGTTGGAAGCTGTAGAGAAGGAAATCAATCGTGTTTCTCCGGTGGTCGAGGACCTTAAGTGTGTTAGTTTGGAGTTACGATCTGCTTGTTTTGCCAAGGATGAGGAGTTTATCTTCATGCATGCTGAAGTGTCTCGTCTCAAGGAGATGACTAGAAAGCTTGAGTCTAAGGAAGTGGATCTGCAAGGTGCGTTGTCTGCTAGTGTGAACCTAAAAAATGAGCTGGATAAGCTGCATGGTGCTCATACTGGGCTTGTTGAGCAGAATGCACAACTGAAGAATGAGAAAGTTGGTACTGAAGTGGCGCTTGCTTCTTGTCAGGCCGATTTCTATAAGCTTGGCTATGTAGACCATCTTCAGGGCAGGCCGTTAGATTATGAGTTTTCCGAGAAAAACTTCGAGACTTTCTCCATTTCTCCAATTGATTTGCTTGATTTCTTGTTTGAGGCTGCCTTTGGTGAAGCAGCTGAAGGTCAGGCAGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
EloA-BP1

Protein Analysis

162

Amino Acids

18.0

Weight (kDa)

4.7

Isoelectric Point (pI)

31.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 348
AclWI GGATC 1 cut(s) 186
AcuI CTGAAG 4 cut(s) 147, 296, 318, 341
AfaI GTAC 1 cut(s) 295
AfiI CCNNNNNNNGG 1 cut(s) 358
AflII CTTAAG 1 cut(s) 56
AluBI AGCT 6 cut(s) 11, 160, 223, 232, 337, 467
AluI AGCT 6 cut(s) 11, 160, 223, 232, 337, 467
Alw21I GWGCWC 1 cut(s) 244
Alw26I GTCTC 3 cut(s) 138, 143, 395
AlwI GGATC 1 cut(s) 186
AoxI GGCC 2 cut(s) 321, 365
ApeKI GCWGC 3 cut(s) 232, 449, 464
Asp700I GAANNNNTTC 1 cut(s) 395
AspLEI GCGC 1 cut(s) 307
AspS9I GGNCC 1 cut(s) 52
AsuHPI GGTGA 1 cut(s) 470
AvaII GGWCC 1 cut(s) 52
BaeI ACNNNNGTAYC 2 cut(s) 285, 318
BarI GAAGNNNNNNTAC 2 cut(s) 339, 371
Bbv12I GWGCWC 1 cut(s) 244
BbvI GCAGC 3 cut(s) 219, 436, 476
BccI CCATC 1 cut(s) 360
BceAI ACGGC 1 cut(s) 352
BcoDI GTCTC 3 cut(s) 138, 143, 395
BfaI CTAG 3 cut(s) 153, 201, 484
BfmI CTRYAG 1 cut(s) 12
BfoI RGCGCY 1 cut(s) 308
BfrI CTTAAG 1 cut(s) 56
BisI GCNGC 3 cut(s) 233, 450, 465
BlsI GCNGC 3 cut(s) 234, 451, 466
Bme18I GGWCC 1 cut(s) 52
BmgT120I GGNCC 1 cut(s) 52
BmrI ACTGGG 1 cut(s) 258
BmuI ACTGGG 1 cut(s) 258
BplI GAGNNNNNCTC 2 cut(s) 392, 424
BpuEI CTTGAG 2 cut(s) 125, 182
BsaJI CCNNGG 1 cut(s) 96
BsaWI WCCGGW 1 cut(s) 40
Bsc4I CCNNNNNNNGG 1 cut(s) 358
Bse1I ACTGG 1 cut(s) 253
BseDI CCNNGG 1 cut(s) 96
BseGI GGATG 1 cut(s) 106
BseLI CCNNNNNNNGG 1 cut(s) 358
BseNI ACTGG 1 cut(s) 253
BseRI GAGGAG 1 cut(s) 119
BseXI GCAGC 3 cut(s) 219, 436, 476
BshFI GGCC 2 cut(s) 323, 367
BsiHKAI GWGCWC 1 cut(s) 244
BsiSI CCGG 1 cut(s) 41
BslI CCNNNNNNNGG 1 cut(s) 358
BsmAI GTCTC 3 cut(s) 138, 143, 395
BsmBI CGTCTC 1 cut(s) 143
BsmI GAATGC 1 cut(s) 271
BsnI GGCC 2 cut(s) 323, 367
Bsp1286I GDGCHC 1 cut(s) 244
Bsp143I GATC 2 cut(s) 80, 178
BspANI GGCC 2 cut(s) 323, 367
BspPI GGATC 1 cut(s) 186
BspTI CTTAAG 1 cut(s) 56
BsrI ACTGG 1 cut(s) 253
BssECI CCNNGG 1 cut(s) 96
BssMI GATC 2 cut(s) 80, 178
BssT1I CCWWGG 1 cut(s) 96
BstAFI CTTAAG 1 cut(s) 56
BstC8I GCNNGC 3 cut(s) 123, 309, 365
BstDEI CTNAG 1 cut(s) 168
BstF5I GGATG 1 cut(s) 106
BstH2I RGCGCY 1 cut(s) 308
BstHHI GCGC 1 cut(s) 307
BstKTI GATC 2 cut(s) 83, 181
BstMAI GTCTC 3 cut(s) 138, 143, 395
BstMBI GATC 2 cut(s) 80, 178
BstMWI GCNNNNNNNGC 4 cut(s) 92, 197, 229, 259
BstNSI RCATGY 1 cut(s) 125
BstSFI CTRYAG 1 cut(s) 12
BstV1I GCAGC 3 cut(s) 219, 436, 476
BstX2I RGATCY 1 cut(s) 178
BstYI RGATCY 1 cut(s) 178
BsuRI GGCC 2 cut(s) 323, 367
BtsCI GGATG 1 cut(s) 106
Cac8I GCNNGC 3 cut(s) 123, 309, 365
CfoI GCGC 1 cut(s) 307
Cfr13I GGNCC 1 cut(s) 52
Csp6I GTAC 1 cut(s) 294
CviAII CATG 3 cut(s) 118, 122, 236
CviQI GTAC 1 cut(s) 294
DdeI CTNAG 1 cut(s) 168
DpnI GATC 2 cut(s) 82, 180
DpnII GATC 2 cut(s) 80, 178
Eco130I CCWWGG 1 cut(s) 96
Eco47I GGWCC 1 cut(s) 52
Eco57I CTGAAG 4 cut(s) 147, 296, 318, 341
EcoO109I RGGNCCY 1 cut(s) 52
EcoT14I CCWWGG 1 cut(s) 96
EcoT22I ATGCAT 1 cut(s) 123
ErhI CCWWGG 1 cut(s) 96
Esp3I CGTCTC 1 cut(s) 143
FaeI CATG 3 cut(s) 121, 125, 239
FaiI YATR 7 cut(s) 119, 123, 237, 246, 333, 345, 378
FalI AAGNNNNNCTT 2 cut(s) 291, 323
FatI CATG 3 cut(s) 117, 121, 235
FblI GTMKAC 1 cut(s) 348
Fnu4HI GCNGC 3 cut(s) 233, 450, 465
FokI GGATG 1 cut(s) 113
Fsp4HI GCNGC 3 cut(s) 233, 450, 465
FspBI CTAG 3 cut(s) 153, 201, 484
GlaI GCGC 1 cut(s) 306
GluI GCNGC 3 cut(s) 233, 450, 465
HaeII RGCGCY 1 cut(s) 308
HaeIII GGCC 2 cut(s) 323, 367
HapII CCGG 1 cut(s) 41
HhaI GCGC 1 cut(s) 307
Hin1II CATG 3 cut(s) 121, 125, 239
Hin6I GCGC 1 cut(s) 305
HinP1I GCGC 1 cut(s) 305
HindIII AAGCTT 2 cut(s) 158, 335
HinfI GANTC 1 cut(s) 164
HpaII CCGG 1 cut(s) 41
HphI GGTGA 1 cut(s) 470
Hpy166II GTNNAC 2 cut(s) 208, 349
Hpy188I TCNGA 1 cut(s) 388
Hpy188III TCNNGA 1 cut(s) 400
Hpy8I GTNNAC 2 cut(s) 208, 349
HpyAV CCTTC 2 cut(s) 13, 464
HpyCH4V TGCA 4 cut(s) 121, 184, 235, 269
HpyF10VI GCNNNNNNNGC 4 cut(s) 92, 197, 229, 259
HpyF3I CTNAG 1 cut(s) 168
Hsp92II CATG 3 cut(s) 121, 125, 239
HspAI GCGC 1 cut(s) 305
Kzo9I GATC 2 cut(s) 80, 178
LpnPI CCDG 7 cut(s) 54, 209, 234, 305, 344, 349, 461
Lsp1109I GCAGC 3 cut(s) 219, 436, 476
MaeI CTAG 3 cut(s) 153, 201, 484
MaeIII GTNAC 1 cut(s) 75
MalI GATC 2 cut(s) 82, 180
MboI GATC 2 cut(s) 80, 178
MboII GAAGA 3 cut(s) 106, 289, 347
MfeI CAATTG 1 cut(s) 420
MflI RGATCY 1 cut(s) 178
MhlI GDGCHC 1 cut(s) 244
MluCI AATT 1 cut(s) 420
MlyI GAGTC 1 cut(s) 173
MnlI CCTC 3 cut(s) 43, 97, 439
Mph1103I ATGCAT 1 cut(s) 123
MroXI GAANNNNTTC 1 cut(s) 395
MseI TTAA 1 cut(s) 57
MspA1I CMGCKG 1 cut(s) 467
MspCI CTTAAG 1 cut(s) 56
MspI CCGG 1 cut(s) 41
MunI CAATTG 1 cut(s) 420
Mva1269I GAATGC 1 cut(s) 271
MwoI GCNNNNNNNGC 4 cut(s) 92, 197, 229, 259
NdeII GATC 2 cut(s) 80, 178
NlaIII CATG 3 cut(s) 121, 125, 239
NsiI ATGCAT 1 cut(s) 123
NspI RCATGY 1 cut(s) 125
PaeI GCATGC 1 cut(s) 125
PctI GAATGC 1 cut(s) 271
PdmI GAANNNNTTC 1 cut(s) 395
PkrI GCNGC 3 cut(s) 234, 451, 466
PleI GAGTC 1 cut(s) 172
PpsI GAGTC 1 cut(s) 172
PpuMI RGGWCCY 1 cut(s) 52
Psp5II RGGWCCY 1 cut(s) 52
PspPI GGNCC 1 cut(s) 52
PspPPI RGGWCCY 1 cut(s) 52
PsuI RGATCY 1 cut(s) 178
PvuII CAGCTG 1 cut(s) 467
RsaI GTAC 1 cut(s) 295
RsaNI GTAC 1 cut(s) 294
SaqAI TTAA 1 cut(s) 57
SatI GCNGC 3 cut(s) 233, 450, 465
Sau3AI GATC 2 cut(s) 80, 178
Sau96I GGNCC 1 cut(s) 52
SchI GAGTC 1 cut(s) 173
SduI GDGCHC 1 cut(s) 244
SfcI CTRYAG 1 cut(s) 12
SinI GGWCC 1 cut(s) 52
SmlI CTYRAG 3 cut(s) 56, 140, 161
SmoI CTYRAG 3 cut(s) 56, 140, 161
SphI GCATGC 1 cut(s) 125
Sse9I AATT 1 cut(s) 420
SspMI CTAG 3 cut(s) 153, 201, 484
StyI CCWWGG 1 cut(s) 96
TaqI TCGA 2 cut(s) 48, 399
TasI AATT 1 cut(s) 420
Tru1I TTAA 1 cut(s) 57
Tru9I TTAA 1 cut(s) 57
TseI GCWGC 3 cut(s) 232, 449, 464
TspDTI ATGAA 1 cut(s) 106
Vha464I CTTAAG 1 cut(s) 56
VpaK11BI GGWCC 1 cut(s) 52
XceI RCATGY 1 cut(s) 125
XmiI GTMKAC 1 cut(s) 348
XmnI GAANNNNTTC 1 cut(s) 395
XspI CTAG 3 cut(s) 153, 201, 484
Zsp2I ATGCAT 1 cut(s) 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.