exonuclease activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1500 |
| AarI | CACCTGC | 2 cut(s) | 204, 2552 |
| AbsI | CCTCGAGG | 1 cut(s) | 95 |
| Acc36I | ACCTGC | 2 cut(s) | 204, 2552 |
| AccB1I | GGYRCC | 3 cut(s) | 582, 880, 1793 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 2345 |
| AccBSI | CCGCTC | 1 cut(s) | 1458 |
| AccI | GTMKAC | 2 cut(s) | 2204, 2509 |
| AccII | CGCG | 1 cut(s) | 2651 |
| AciI | CCGC | 7 cut(s) | 638, 947, 1456, 2011, 2496, 2754, 2819 |
| AclWI | GGATC | 2 cut(s) | 428, 1369 |
| AcoI | YGGCCR | 1 cut(s) | 1245 |
| AcsI | RAATTY | 7 cut(s) | 271, 475, 962, 1268, 1933, 2617, 2793 |
| AcuI | CTGAAG | 2 cut(s) | 1610, 1923 |
| AcvI | CACGTG | 1 cut(s) | 131 |
| AfaI | GTAC | 8 cut(s) | 92, 576, 940, 1414, 1624, 2353, 2676, 2775 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 417, 762, 1636, 1803, 1804, 2345, 2379 |
| AflII | CTTAAG | 2 cut(s) | 301, 1538 |
| AflIII | ACRYGT | 2 cut(s) | 130, 889 |
| AjiI | CACGTC | 1 cut(s) | 2463 |
| AjnI | CCWGG | 3 cut(s) | 551, 980, 1797 |
| Alw21I | GWGCWC | 1 cut(s) | 2308 |
| Alw26I | GTCTC | 6 cut(s) | 559, 746, 1006, 1322, 1450, 1670 |
| Alw44I | GTGCAC | 1 cut(s) | 2304 |
| AlwI | GGATC | 2 cut(s) | 428, 1369 |
| Ama87I | CYCGRG | 2 cut(s) | 95, 1634 |
| AoxI | GGCC | 5 cut(s) | 541, 555, 690, 773, 1245 |
| ApaLI | GTGCAC | 1 cut(s) | 2304 |
| ApoI | RAATTY | 7 cut(s) | 271, 475, 962, 1268, 1933, 2617, 2793 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 785, 817 |
| AspLEI | GCGC | 1 cut(s) | 1825 |
| AspS9I | GGNCC | 6 cut(s) | 541, 555, 635, 691, 726, 2383 |
| AsuC2I | CCSGG | 1 cut(s) | 86 |
| AsuHPI | GGTGA | 7 cut(s) | 108, 127, 813, 1670, 1829, 2189, 2576 |
| AsuII | TTCGAA | 1 cut(s) | 2474 |
| AsuNHI | GCTAGC | 1 cut(s) | 2234 |
| AvaI | CYCGRG | 2 cut(s) | 95, 1634 |
| AvaII | GGWCC | 3 cut(s) | 635, 726, 2383 |
| AxyI | CCTNAGG | 1 cut(s) | 2066 |
| BaeGI | GKGCMC | 2 cut(s) | 1798, 2308 |
| BanI | GGYRCC | 3 cut(s) | 582, 880, 1793 |
| BanII | GRGCYC | 2 cut(s) | 616, 688 |
| BbrPI | CACGTG | 1 cut(s) | 131 |
| BbsI | GAAGAC | 3 cut(s) | 436, 1364, 1755 |
| Bbv12I | GWGCWC | 1 cut(s) | 2308 |
| BbvCI | CCTCAGC | 1 cut(s) | 1842 |
| BccI | CCATC | 7 cut(s) | 34, 305, 511, 1823, 1973, 2347, 2429 |
| BceAI | ACGGC | 2 cut(s) | 542, 2252 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1715, 1749 |
| BciT130I | CCWGG | 3 cut(s) | 553, 982, 1799 |
| BciVI | GTATCC | 1 cut(s) | 1183 |
| BclI | TGATCA | 1 cut(s) | 1180 |
| BcnI | CCSGG | 1 cut(s) | 86 |
| BcoDI | GTCTC | 6 cut(s) | 559, 746, 1006, 1322, 1450, 1670 |
| BfaI | CTAG | 9 cut(s) | 389, 1812, 1928, 2235, 2330, 2379, 2441, 2823, 2842 |
| BfmI | CTRYAG | 2 cut(s) | 1610, 2110 |
| BfoI | RGCGCY | 1 cut(s) | 1826 |
| BfrI | CTTAAG | 2 cut(s) | 301, 1538 |
| BfuAI | ACCTGC | 2 cut(s) | 204, 2552 |
| BfuI | GTATCC | 1 cut(s) | 1183 |
| BglII | AGATCT | 2 cut(s) | 223, 1429 |
| BlpI | GCTNAGC | 2 cut(s) | 786, 2450 |
| BmcAI | AGTACT | 1 cut(s) | 1624 |
| Bme1390I | CCNGG | 4 cut(s) | 86, 553, 982, 1799 |
| Bme18I | GGWCC | 3 cut(s) | 635, 726, 2383 |
| BmeT110I | CYCGRG | 2 cut(s) | 95, 1634 |
| BmgBI | CACGTC | 1 cut(s) | 2463 |
| BmgT120I | GGNCC | 6 cut(s) | 541, 555, 635, 691, 726, 2383 |
| BmiI | GGNNCC | 7 cut(s) | 584, 615, 727, 882, 1795, 2384, 2809 |
| BmrFI | CCNGG | 4 cut(s) | 86, 553, 982, 1799 |
| BmrI | ACTGGG | 1 cut(s) | 1371 |
| BmsI | GCATC | 5 cut(s) | 472, 1128, 1857, 2137, 2437 |
| BmtI | GCTAGC | 1 cut(s) | 2238 |
| BmuI | ACTGGG | 1 cut(s) | 1371 |
| BpiI | GAAGAC | 3 cut(s) | 436, 1364, 1755 |
| BpmI | CTGGAG | 1 cut(s) | 1046 |
| Bpu10I | CCTNAGC | 3 cut(s) | 687, 1059, 1842 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 786, 2450 |
| Bpu14I | TTCGAA | 1 cut(s) | 2474 |
| BpuEI | CTTGAG | 3 cut(s) | 1346, 1454, 1530 |
| BpuMI | CCSGG | 1 cut(s) | 86 |
| BsaAI | YACGTR | 1 cut(s) | 131 |
| BsaI | GGTCTC | 1 cut(s) | 746 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 417, 762, 1636, 1803, 1804, 2345, 2379 |
| Bse1I | ACTGG | 3 cut(s) | 1029, 1366, 1719 |
| Bse21I | CCTNAGG | 1 cut(s) | 2066 |
| Bse3DI | GCAATG | 2 cut(s) | 1461, 2694 |
| BseBI | CCWGG | 3 cut(s) | 553, 982, 1799 |
| BseGI | GGATG | 7 cut(s) | 45, 60, 1483, 1834, 1894, 2339, 2456 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 417, 762, 1636, 1803, 1804, 2345, 2379 |
| BseMI | GCAATG | 2 cut(s) | 1461, 2694 |
| BseNI | ACTGG | 3 cut(s) | 1029, 1366, 1719 |
| BseRI | GAGGAG | 5 cut(s) | 375, 577, 1619, 1652, 2359 |
| BseSI | GKGCMC | 2 cut(s) | 1798, 2308 |
| BseYI | CCCAGC | 3 cut(s) | 609, 681, 762 |
| BsgI | GTGCAG | 3 cut(s) | 1416, 2172, 2580 |
| Bsh1236I | CGCG | 1 cut(s) | 2651 |
| BshFI | GGCC | 5 cut(s) | 543, 557, 692, 775, 1247 |
| BshNI | GGYRCC | 3 cut(s) | 582, 880, 1793 |
| BsiHKAI | GWGCWC | 1 cut(s) | 2308 |
| BsiHKCI | CYCGRG | 2 cut(s) | 95, 1634 |
| BsiSI | CCGG | 3 cut(s) | 86, 1782, 1826 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 417, 762, 1636, 1803, 1804, 2345, 2379 |
| BsmAI | GTCTC | 6 cut(s) | 559, 746, 1006, 1322, 1450, 1670 |
| BsnI | GGCC | 5 cut(s) | 543, 557, 692, 775, 1247 |
| Bso31I | GGTCTC | 1 cut(s) | 746 |
| BsoBI | CYCGRG | 2 cut(s) | 95, 1634 |
| Bsp119I | TTCGAA | 1 cut(s) | 2474 |
| Bsp1286I | GDGCHC | 4 cut(s) | 616, 688, 1798, 2308 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 786, 2450 |
| Bsp19I | CCATGG | 1 cut(s) | 483 |
| Bsp68I | TCGCGA | 1 cut(s) | 2651 |
| BspACI | CCGC | 7 cut(s) | 638, 947, 1456, 2011, 2496, 2754, 2819 |
| BspANI | GGCC | 5 cut(s) | 543, 557, 692, 775, 1247 |
| BspFNI | CGCG | 1 cut(s) | 2651 |
| BspHI | TCATGA | 1 cut(s) | 2241 |
| BspLI | GGNNCC | 7 cut(s) | 584, 615, 727, 882, 1795, 2384, 2809 |
| BspMAI | CTGCAG | 1 cut(s) | 1614 |
| BspMI | ACCTGC | 2 cut(s) | 204, 2552 |
| BspOI | GCTAGC | 1 cut(s) | 2238 |
| BspPI | GGATC | 2 cut(s) | 428, 1369 |
| BspT104I | TTCGAA | 1 cut(s) | 2474 |
| BspT107I | GGYRCC | 3 cut(s) | 582, 880, 1793 |
| BspTI | CTTAAG | 2 cut(s) | 301, 1538 |
| BspTNI | GGTCTC | 1 cut(s) | 746 |
| BsrBI | CCGCTC | 1 cut(s) | 1458 |
| BsrDI | GCAATG | 2 cut(s) | 1461, 2694 |
| BsrI | ACTGG | 3 cut(s) | 1029, 1366, 1719 |
| BssNAI | GTATAC | 1 cut(s) | 2510 |
| BssT1I | CCWWGG | 5 cut(s) | 483, 855, 1194, 1203, 2642 |
| Bst1107I | GTATAC | 1 cut(s) | 2510 |
| Bst2UI | CCWGG | 3 cut(s) | 553, 982, 1799 |
| Bst4CI | ACNGT | 5 cut(s) | 501, 1222, 1586, 2209, 2703 |
| Bst6I | CTCTTC | 2 cut(s) | 738, 842 |
| BstAFI | CTTAAG | 2 cut(s) | 301, 1538 |
| BstBAI | YACGTR | 1 cut(s) | 131 |
| BstBI | TTCGAA | 1 cut(s) | 2474 |
| BstDSI | CCRYGG | 2 cut(s) | 483, 500 |
| BstEII | GGTNACC | 1 cut(s) | 12 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1634 |
| BstF5I | GGATG | 7 cut(s) | 45, 60, 1483, 1834, 1894, 2339, 2456 |
| BstFNI | CGCG | 1 cut(s) | 2651 |
| BstH2I | RGCGCY | 1 cut(s) | 1826 |
| BstHHI | GCGC | 1 cut(s) | 1825 |
| BstMAI | GTCTC | 6 cut(s) | 559, 746, 1006, 1322, 1450, 1670 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 540, 1130, 1232, 2015, 2125, 2762, 2816 |
| BstNI | CCWGG | 3 cut(s) | 553, 982, 1799 |
| BstNSI | RCATGY | 4 cut(s) | 681, 893, 2128, 2203 |
| BstPI | GGTNACC | 1 cut(s) | 12 |
| BstSCI | CCNGG | 4 cut(s) | 84, 551, 980, 1797 |
| BstSFI | CTRYAG | 2 cut(s) | 1610, 2110 |
| BstSLI | GKGCMC | 2 cut(s) | 1798, 2308 |
| BstUI | CGCG | 1 cut(s) | 2651 |
| BstV2I | GAAGAC | 3 cut(s) | 436, 1364, 1755 |
| BstX2I | RGATCY | 2 cut(s) | 223, 1429 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1210 |
| BstYI | RGATCY | 2 cut(s) | 223, 1429 |
| BstZ17I | GTATAC | 1 cut(s) | 2510 |
| Bsu36I | CCTNAGG | 1 cut(s) | 2066 |
| BsuI | GTATCC | 1 cut(s) | 1183 |
| BsuRI | GGCC | 5 cut(s) | 543, 557, 692, 775, 1247 |
| BtgI | CCRYGG | 2 cut(s) | 483, 500 |
| BtrI | CACGTC | 1 cut(s) | 2463 |
| BtsCI | GGATG | 7 cut(s) | 45, 60, 1483, 1834, 1894, 2339, 2456 |
| BtsI | GCAGTG | 1 cut(s) | 64 |
| BtsIMutI | CAGTG | 4 cut(s) | 64, 497, 2214, 2699 |
| BtuMI | TCGCGA | 1 cut(s) | 2651 |
| BveI | ACCTGC | 2 cut(s) | 204, 2552 |
| CciI | TCATGA | 1 cut(s) | 2241 |
| CfoI | GCGC | 1 cut(s) | 1825 |
| Cfr13I | GGNCC | 6 cut(s) | 541, 555, 635, 691, 726, 2383 |
| CseI | GACGC | 2 cut(s) | 1159, 2422 |
| Csp6I | GTAC | 8 cut(s) | 91, 575, 939, 1413, 1623, 2352, 2675, 2774 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1203, 1238 |
| CviQI | GTAC | 8 cut(s) | 91, 575, 939, 1413, 1623, 2352, 2675, 2774 |
| EaeI | YGGCCR | 1 cut(s) | 1245 |
| Eam1104I | CTCTTC | 2 cut(s) | 738, 842 |
| EarI | CTCTTC | 2 cut(s) | 738, 842 |
| Eco130I | CCWWGG | 5 cut(s) | 483, 855, 1194, 1203, 2642 |
| Eco24I | GRGCYC | 2 cut(s) | 616, 688 |
| Eco31I | GGTCTC | 1 cut(s) | 746 |
| Eco32I | GATATC | 1 cut(s) | 2713 |
| Eco47I | GGWCC | 3 cut(s) | 635, 726, 2383 |
| Eco57I | CTGAAG | 2 cut(s) | 1610, 1923 |
| Eco72I | CACGTG | 1 cut(s) | 131 |
| Eco81I | CCTNAGG | 1 cut(s) | 2066 |
| Eco88I | CYCGRG | 2 cut(s) | 95, 1634 |
| Eco91I | GGTNACC | 1 cut(s) | 12 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1634 |
| EcoO65I | GGTNACC | 1 cut(s) | 12 |
| EcoRI | GAATTC | 1 cut(s) | 2617 |
| EcoRII | CCWGG | 3 cut(s) | 551, 980, 1797 |
| EcoRV | GATATC | 1 cut(s) | 2713 |
| EcoT14I | CCWWGG | 5 cut(s) | 483, 855, 1194, 1203, 2642 |
| EcoT22I | ATGCAT | 1 cut(s) | 2130 |
| EcoT38I | GRGCYC | 2 cut(s) | 616, 688 |
| ErhI | CCWWGG | 5 cut(s) | 483, 855, 1194, 1203, 2642 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 284, 316 |
| FauI | CCCGC | 3 cut(s) | 940, 2018, 2503 |
| FauNDI | CATATG | 1 cut(s) | 2788 |
| FbaI | TGATCA | 1 cut(s) | 1180 |
| FblI | GTMKAC | 2 cut(s) | 2204, 2509 |
| FokI | GGATG | 7 cut(s) | 52, 67, 1490, 1841, 1901, 2326, 2443 |
| FriOI | GRGCYC | 2 cut(s) | 616, 688 |
| FspBI | CTAG | 9 cut(s) | 389, 1812, 1928, 2235, 2330, 2379, 2441, 2823, 2842 |
| GlaI | GCGC | 1 cut(s) | 1824 |
| GsaI | CCCAGC | 3 cut(s) | 613, 685, 766 |
| GsuI | CTGGAG | 1 cut(s) | 1046 |
| HaeII | RGCGCY | 1 cut(s) | 1826 |
| HaeIII | GGCC | 5 cut(s) | 543, 557, 692, 775, 1247 |
| HapII | CCGG | 3 cut(s) | 86, 1782, 1826 |
| HgaI | GACGC | 2 cut(s) | 1159, 2422 |
| HhaI | GCGC | 1 cut(s) | 1825 |
| Hin6I | GCGC | 1 cut(s) | 1823 |
| HinP1I | GCGC | 1 cut(s) | 1823 |
| HincII | GTYRAC | 2 cut(s) | 508, 2205 |
| HindII | GTYRAC | 2 cut(s) | 508, 2205 |
| HindIII | AAGCTT | 3 cut(s) | 361, 1233, 2181 |
| HinfI | GANTC | 8 cut(s) | 649, 914, 1423, 1617, 1693, 1760, 1973, 2406 |
| HpaII | CCGG | 3 cut(s) | 86, 1782, 1826 |
| HphI | GGTGA | 7 cut(s) | 108, 127, 813, 1670, 1829, 2189, 2576 |
| Hpy99I | CGWCG | 1 cut(s) | 2416 |
| HpyCH4III | ACNGT | 5 cut(s) | 501, 1222, 1586, 2209, 2703 |
| HpyCH4IV | ACGT | 4 cut(s) | 130, 796, 2357, 2462 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 540, 1130, 1232, 2015, 2125, 2762, 2816 |
| HpySE526I | ACGT | 4 cut(s) | 130, 796, 2357, 2462 |
| HspAI | GCGC | 1 cut(s) | 1823 |
| Ksp22I | TGATCA | 1 cut(s) | 1180 |
| LmnI | GCTCC | 8 cut(s) | 170, 460, 468, 619, 1458, 1463, 1606, 2807 |
| LweI | GCATC | 5 cut(s) | 472, 1128, 1857, 2137, 2437 |
| MaeI | CTAG | 9 cut(s) | 389, 1812, 1928, 2235, 2330, 2379, 2441, 2823, 2842 |
| MaeII | ACGT | 4 cut(s) | 130, 796, 2357, 2462 |
| MaeIII | GTNAC | 3 cut(s) | 12, 133, 2664 |
| MbiI | CCGCTC | 1 cut(s) | 1458 |
| MflI | RGATCY | 2 cut(s) | 223, 1429 |
| MhlI | GDGCHC | 4 cut(s) | 616, 688, 1798, 2308 |
| MlyI | GAGTC | 3 cut(s) | 908, 1702, 1769 |
| MmeI | TCCRAC | 1 cut(s) | 1533 |
| Mph1103I | ATGCAT | 1 cut(s) | 2130 |
| MseI | TTAA | 5 cut(s) | 302, 314, 449, 1539, 1937 |
| MspA1I | CMGCKG | 1 cut(s) | 954 |
| MspCI | CTTAAG | 2 cut(s) | 301, 1538 |
| MspI | CCGG | 3 cut(s) | 86, 1782, 1826 |
| MspR9I | CCNGG | 4 cut(s) | 86, 553, 982, 1799 |
| MvaI | CCWGG | 3 cut(s) | 553, 982, 1799 |
| MvnI | CGCG | 1 cut(s) | 2651 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 540, 1130, 1232, 2015, 2125, 2762, 2816 |
| NciI | CCSGG | 1 cut(s) | 86 |
| NcoI | CCATGG | 1 cut(s) | 483 |
| NdeI | CATATG | 1 cut(s) | 2788 |
| NheI | GCTAGC | 1 cut(s) | 2234 |
| NlaIV | GGNNCC | 7 cut(s) | 584, 615, 727, 882, 1795, 2384, 2809 |
| NmeAIII | GCCGAG | 1 cut(s) | 1223 |
| NmuCI | GTSAC | 2 cut(s) | 133, 2664 |
| NruI | TCGCGA | 1 cut(s) | 2651 |
| NsiI | ATGCAT | 1 cut(s) | 2130 |
| NspI | RCATGY | 4 cut(s) | 681, 893, 2128, 2203 |
| NspV | TTCGAA | 1 cut(s) | 2474 |
| PaeI | GCATGC | 1 cut(s) | 2128 |
| PaeR7I | CTCGAG | 2 cut(s) | 95, 1634 |
| PagI | TCATGA | 1 cut(s) | 2241 |
| PaqCI | CACCTGC | 2 cut(s) | 204, 2552 |
| PciI | ACATGT | 1 cut(s) | 889 |
| PfeI | GAWTC | 5 cut(s) | 649, 1423, 1617, 1973, 2406 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 2345 |
| PleI | GAGTC | 3 cut(s) | 908, 1701, 1768 |
| PmaCI | CACGTG | 1 cut(s) | 131 |
| PmlI | CACGTG | 1 cut(s) | 131 |
| PpsI | GAGTC | 3 cut(s) | 908, 1701, 1768 |
| Ppu21I | YACGTR | 1 cut(s) | 131 |
| PscI | ACATGT | 1 cut(s) | 889 |
| PsiI | TTATAA | 1 cut(s) | 1500 |
| Psp6I | CCWGG | 3 cut(s) | 551, 980, 1797 |
| PspCI | CACGTG | 1 cut(s) | 131 |
| PspEI | GGTNACC | 1 cut(s) | 12 |
| PspFI | CCCAGC | 3 cut(s) | 609, 681, 762 |
| PspGI | CCWGG | 3 cut(s) | 551, 980, 1797 |
| PspN4I | GGNNCC | 7 cut(s) | 584, 615, 727, 882, 1795, 2384, 2809 |
| PspPI | GGNCC | 6 cut(s) | 541, 555, 635, 691, 726, 2383 |
| PspXI | VCTCGAGB | 2 cut(s) | 95, 1634 |
| PstI | CTGCAG | 1 cut(s) | 1614 |
| PsuI | RGATCY | 2 cut(s) | 223, 1429 |
| PvuII | CAGCTG | 1 cut(s) | 954 |
| RruI | TCGCGA | 1 cut(s) | 2651 |
| RsaI | GTAC | 8 cut(s) | 92, 576, 940, 1414, 1624, 2353, 2676, 2775 |
| RsaNI | GTAC | 8 cut(s) | 91, 575, 939, 1413, 1623, 2352, 2675, 2774 |
| SalI | GTCGAC | 1 cut(s) | 2203 |
| SaqAI | TTAA | 5 cut(s) | 302, 314, 449, 1539, 1937 |
| Sau96I | GGNCC | 6 cut(s) | 541, 555, 635, 691, 726, 2383 |
| ScaI | AGTACT | 1 cut(s) | 1624 |
| SchI | GAGTC | 3 cut(s) | 908, 1702, 1769 |
| ScrFI | CCNGG | 4 cut(s) | 86, 553, 982, 1799 |
| SduI | GDGCHC | 4 cut(s) | 616, 688, 1798, 2308 |
| SfaNI | GCATC | 5 cut(s) | 472, 1128, 1857, 2137, 2437 |
| SfcI | CTRYAG | 2 cut(s) | 1610, 2110 |
| Sfr274I | CTCGAG | 2 cut(s) | 95, 1634 |
| SfuI | TTCGAA | 1 cut(s) | 2474 |
| SinI | GGWCC | 3 cut(s) | 635, 726, 2383 |
| SlaI | CTCGAG | 2 cut(s) | 95, 1634 |
| SmlI | CTYRAG | 7 cut(s) | 95, 301, 1325, 1433, 1538, 1545, 1634 |
| SmoI | CTYRAG | 7 cut(s) | 95, 301, 1325, 1433, 1538, 1545, 1634 |
| SphI | GCATGC | 1 cut(s) | 2128 |
| SsiI | CCGC | 7 cut(s) | 638, 947, 1456, 2011, 2496, 2754, 2819 |
| SspMI | CTAG | 9 cut(s) | 389, 1812, 1928, 2235, 2330, 2379, 2441, 2823, 2842 |
| StyD4I | CCNGG | 4 cut(s) | 84, 551, 980, 1797 |
| StyI | CCWWGG | 5 cut(s) | 483, 855, 1194, 1203, 2642 |
| TaaI | ACNGT | 5 cut(s) | 501, 1222, 1586, 2209, 2703 |
| TaiI | ACGT | 4 cut(s) | 133, 799, 2360, 2465 |
| TaqI | TCGA | 9 cut(s) | 96, 1635, 1735, 1971, 2188, 2204, 2256, 2474, 2709 |
| TaqII | GACCGA | 1 cut(s) | 770 |
| TatI | WGTACW | 2 cut(s) | 1622, 2773 |
| TauI | GCSGC | 1 cut(s) | 2822 |
| TfiI | GAWTC | 5 cut(s) | 649, 1423, 1617, 1973, 2406 |
| Tru1I | TTAA | 5 cut(s) | 302, 314, 449, 1539, 1937 |
| Tru9I | TTAA | 5 cut(s) | 302, 314, 449, 1539, 1937 |
| TscAI | CASTG | 4 cut(s) | 71, 497, 2214, 2706 |
| TseFI | GTSAC | 2 cut(s) | 133, 2664 |
| Tsp45I | GTSAC | 2 cut(s) | 133, 2664 |
| TspDTI | ATGAA | 8 cut(s) | 860, 1404, 1494, 2230, 2714, 2786, 2805, 2844 |
| TspGWI | ACGGA | 1 cut(s) | 789 |
| TspRI | CASTG | 4 cut(s) | 71, 497, 2214, 2706 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 2345 |
| Vha464I | CTTAAG | 2 cut(s) | 301, 1538 |
| VneI | GTGCAC | 1 cut(s) | 2304 |
| VpaK11BI | GGWCC | 3 cut(s) | 635, 726, 2383 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1634 |
| XapI | RAATTY | 7 cut(s) | 271, 475, 962, 1268, 1933, 2617, 2793 |
| XceI | RCATGY | 4 cut(s) | 681, 893, 2128, 2203 |
| XhoI | CTCGAG | 2 cut(s) | 95, 1634 |
| XmiI | GTMKAC | 2 cut(s) | 2204, 2509 |
| XspI | CTAG | 9 cut(s) | 389, 1812, 1928, 2235, 2330, 2379, 2441, 2823, 2842 |
| ZrmI | AGTACT | 1 cut(s) | 1624 |
| Zsp2I | ATGCAT | 1 cut(s) | 2130 |