pycom06g01870
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0003348
Physical Location & Seq
Reverse (-)
2375043 .. 2381091
6049 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 519 bp
ATGGCGATGCATAGATTATTCTCCAAGCTTCGCTCTTTCTCTGTGCACTCAGCCCAAACTCTCCCATCATCTCTCTCTTCTCCTTCATTGCATCGGTCTTGCCCTCCGCTGCTTCACCAGCCTCTCCCTTTCGCATCAATTGATAACAGACGGATCGTCAGGTCCCTTTTCAGTGCCCATTCACCGACCCAACCTTCACTGGCTGTCACTGCTCTTCGCTCCTCACCATTCCCTTTCCCGATGGTTCAAGTGCGGCATGTTTCGTCAAGGGAGCGTAAGAAGAGGAGAAAGCCGATGACTCCAGTCACATCCAAGTTAAAGAAAACCAAAATGAAGTCTTACTCGTCGTACAAGTCCCGGTTTAGGACAATGAATGATGGGACTATTCGGCGGTGGAGGGCGGGCAAGCGGCACAATGCCCATTCGAAGTCGAAGATATCTAAGCGTAGACTCAGAGTGCCAGCCCTTGTCCATCCTGCTTATGCTACAGTGATGAAGAAGCTCAATTTTTGCGGTTAA

Protein Analysis

173

Amino Acids

19.7

Weight (kDa)

12.0

Isoelectric Point (pI)

85.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L35p PF01632 110 - 168 2.6e-15 Ribosomal protein L35
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 448
AciI CCGC 6 cut(s) 107, 253, 391, 401, 409, 513
AclWI GGATC 1 cut(s) 161
AfaI GTAC 1 cut(s) 350
AfiI CCNNNNNNNGG 1 cut(s) 363
AgsI TTSAA 1 cut(s) 248
AhdI GACNNNNNGTC 1 cut(s) 155
AluBI AGCT 2 cut(s) 28, 502
AluI AGCT 2 cut(s) 28, 502
Alw21I GWGCWC 1 cut(s) 48
Alw44I GTGCAC 1 cut(s) 44
AlwI GGATC 1 cut(s) 161
ApaLI GTGCAC 1 cut(s) 44
ApeKI GCWGC 1 cut(s) 109
AspS9I GGNCC 1 cut(s) 162
AsuC2I CCSGG 1 cut(s) 358
AsuHPI GGTGA 3 cut(s) 107, 174, 216
AsuII TTCGAA 1 cut(s) 425
AvaII GGWCC 1 cut(s) 162
BaeGI GKGCMC 2 cut(s) 48, 178
Bbv12I GWGCWC 1 cut(s) 48
BbvI GCAGC 1 cut(s) 96
BccI CCATC 4 cut(s) 73, 235, 371, 480
BcnI CCSGG 1 cut(s) 358
BfmI CTRYAG 1 cut(s) 486
BisI GCNGC 3 cut(s) 110, 254, 410
BlsI GCNGC 3 cut(s) 111, 255, 411
Bme1390I CCNGG 1 cut(s) 358
Bme18I GGWCC 1 cut(s) 162
BmeRI GACNNNNNGTC 1 cut(s) 155
BmgT120I GGNCC 1 cut(s) 162
BmiI GGNNCC 1 cut(s) 164
BmrFI CCNGG 1 cut(s) 358
BmsI GCATC 2 cut(s) 100, 143
BoxI GACNNNNGTC 1 cut(s) 302
BpmI CTGGAG 1 cut(s) 285
Bpu14I TTCGAA 1 cut(s) 425
BpuMI CCSGG 1 cut(s) 358
Bsc4I CCNNNNNNNGG 1 cut(s) 363
Bse1I ACTGG 2 cut(s) 204, 302
Bse3DI GCAATG 1 cut(s) 86
BseGI GGATG 2 cut(s) 308, 472
BseLI CCNNNNNNNGG 1 cut(s) 363
BseMI GCAATG 1 cut(s) 86
BseMII CTCAG 2 cut(s) 63, 466
BseNI ACTGG 2 cut(s) 204, 302
BseRI GAGGAG 2 cut(s) 211, 298
BseSI GKGCMC 2 cut(s) 48, 178
BseXI GCAGC 1 cut(s) 96
BsiHKAI GWGCWC 1 cut(s) 48
BsiSI CCGG 1 cut(s) 358
BslFI GGGAC 3 cut(s) 148, 340, 394
BslI CCNNNNNNNGG 1 cut(s) 363
BsmFI GGGAC 3 cut(s) 148, 340, 394
Bsp119I TTCGAA 1 cut(s) 425
Bsp1286I GDGCHC 2 cut(s) 48, 178
Bsp143I GATC 1 cut(s) 153
BspACI CCGC 6 cut(s) 107, 253, 391, 401, 409, 513
BspCNI CTCAG 2 cut(s) 62, 465
BspLI GGNNCC 1 cut(s) 164
BspPI GGATC 1 cut(s) 161
BspQI GCTCTTC 1 cut(s) 219
BspT104I TTCGAA 1 cut(s) 425
BsrDI GCAATG 1 cut(s) 86
BsrI ACTGG 2 cut(s) 204, 302
BssMI GATC 1 cut(s) 153
Bst4CI ACNGT 1 cut(s) 490
Bst6I CTCTTC 3 cut(s) 82, 219, 275
BstBI TTCGAA 1 cut(s) 425
BstC8I GCNNGC 3 cut(s) 403, 407, 462
BstDEI CTNAG 3 cut(s) 49, 441, 452
BstF5I GGATG 2 cut(s) 308, 472
BstKTI GATC 1 cut(s) 156
BstMBI GATC 1 cut(s) 153
BstMWI GCNNNNNNNGC 2 cut(s) 118, 209
BstNSI RCATGY 1 cut(s) 260
BstPAI GACNNNNGTC 1 cut(s) 302
BstSCI CCNGG 1 cut(s) 356
BstSFI CTRYAG 1 cut(s) 486
BstSLI GKGCMC 2 cut(s) 48, 178
BstV1I GCAGC 1 cut(s) 96
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 2 cut(s) 308, 472
BtsI GCAGTG 1 cut(s) 207
BtsIMutI CAGTG 4 cut(s) 178, 197, 207, 495
Cac8I GCNNGC 3 cut(s) 403, 407, 462
Cfr13I GGNCC 1 cut(s) 162
Csp6I GTAC 1 cut(s) 349
CviAII CATG 1 cut(s) 257
CviJI RGCY 7 cut(s) 28, 53, 121, 203, 292, 464, 502
CviKI_1 RGCY 7 cut(s) 28, 53, 121, 203, 292, 464, 502
CviQI GTAC 1 cut(s) 349
DdeI CTNAG 3 cut(s) 49, 441, 452
DpnI GATC 1 cut(s) 155
DpnII GATC 1 cut(s) 153
DriI GACNNNNNGTC 1 cut(s) 155
Eam1104I CTCTTC 3 cut(s) 82, 219, 275
Eam1105I GACNNNNNGTC 1 cut(s) 155
EarI CTCTTC 3 cut(s) 82, 219, 275
Eco32I GATATC 1 cut(s) 438
Eco47I GGWCC 1 cut(s) 162
EcoO109I RGGNCCY 1 cut(s) 162
EcoRV GATATC 1 cut(s) 438
EcoT22I ATGCAT 1 cut(s) 12
FaeI CATG 1 cut(s) 260
FaiI YATR 3 cut(s) 12, 258, 483
FaqI GGGAC 3 cut(s) 148, 340, 394
FatI CATG 1 cut(s) 256
FauI CCCGC 1 cut(s) 394
FblI GTMKAC 1 cut(s) 448
Fnu4HI GCNGC 3 cut(s) 110, 254, 410
FokI GGATG 2 cut(s) 295, 459
Fsp4HI GCNGC 3 cut(s) 110, 254, 410
GluI GCNGC 3 cut(s) 110, 254, 410
GsuI CTGGAG 1 cut(s) 285
HapII CCGG 1 cut(s) 358
Hin1II CATG 1 cut(s) 260
HindIII AAGCTT 1 cut(s) 26
HinfI GANTC 2 cut(s) 298, 450
HpaII CCGG 1 cut(s) 358
HphI GGTGA 3 cut(s) 107, 174, 216
Hpy166II GTNNAC 2 cut(s) 46, 449
Hpy188I TCNGA 1 cut(s) 455
Hpy188III TCNNGA 1 cut(s) 238
Hpy8I GTNNAC 2 cut(s) 46, 449
Hpy99I CGWCG 1 cut(s) 349
HpyAV CCTTC 2 cut(s) 93, 204
HpyCH4III ACNGT 1 cut(s) 490
HpyCH4V TGCA 3 cut(s) 10, 46, 91
HpyF10VI GCNNNNNNNGC 2 cut(s) 118, 209
HpyF3I CTNAG 3 cut(s) 49, 441, 452
Hsp92II CATG 1 cut(s) 260
Kzo9I GATC 1 cut(s) 153
LguI GCTCTTC 1 cut(s) 219
LmnI GCTCC 2 cut(s) 224, 271
LpnPI CCDG 7 cut(s) 131, 145, 185, 315, 371, 474, 489
Lsp1109I GCAGC 1 cut(s) 96
LweI GCATC 2 cut(s) 100, 143
MaeIII GTNAC 2 cut(s) 205, 304
MalI GATC 1 cut(s) 155
MboI GATC 1 cut(s) 153
MboII GAAGA 5 cut(s) 69, 206, 292, 445, 508
MfeI CAATTG 1 cut(s) 138
MhlI GDGCHC 2 cut(s) 48, 178
MluCI AATT 2 cut(s) 138, 505
MlyI GAGTC 2 cut(s) 292, 444
MnlI CCTC 5 cut(s) 114, 132, 232, 276, 390
Mph1103I ATGCAT 1 cut(s) 12
MseI TTAA 2 cut(s) 317, 517
MspA1I CMGCKG 1 cut(s) 109
MspI CCGG 1 cut(s) 358
MspR9I CCNGG 1 cut(s) 358
MunI CAATTG 1 cut(s) 138
MwoI GCNNNNNNNGC 2 cut(s) 118, 209
NciI CCSGG 1 cut(s) 358
NdeII GATC 1 cut(s) 153
NlaIII CATG 1 cut(s) 260
NlaIV GGNNCC 1 cut(s) 164
NmuCI GTSAC 2 cut(s) 205, 304
NsiI ATGCAT 1 cut(s) 12
NspI RCATGY 1 cut(s) 260
NspV TTCGAA 1 cut(s) 425
PciSI GCTCTTC 1 cut(s) 219
PkrI GCNGC 3 cut(s) 111, 255, 411
PleI GAGTC 2 cut(s) 292, 444
PpsI GAGTC 2 cut(s) 292, 444
PpuMI RGGWCCY 1 cut(s) 162
PshAI GACNNNNGTC 1 cut(s) 302
Psp5II RGGWCCY 1 cut(s) 162
PspN4I GGNNCC 1 cut(s) 164
PspPI GGNCC 1 cut(s) 162
PspPPI RGGWCCY 1 cut(s) 162
RsaI GTAC 1 cut(s) 350
RsaNI GTAC 1 cut(s) 349
SapI GCTCTTC 1 cut(s) 219
SaqAI TTAA 2 cut(s) 317, 517
SatI GCNGC 3 cut(s) 110, 254, 410
Sau3AI GATC 1 cut(s) 153
Sau96I GGNCC 1 cut(s) 162
SchI GAGTC 2 cut(s) 292, 444
ScrFI CCNGG 1 cut(s) 358
SduI GDGCHC 2 cut(s) 48, 178
SetI ASST 4 cut(s) 30, 164, 196, 504
SfaNI GCATC 2 cut(s) 100, 143
SfcI CTRYAG 1 cut(s) 486
SfuI TTCGAA 1 cut(s) 425
SinI GGWCC 1 cut(s) 162
Sse9I AATT 2 cut(s) 138, 505
SsiI CCGC 6 cut(s) 107, 253, 391, 401, 409, 513
StyD4I CCNGG 1 cut(s) 356
TaaI ACNGT 1 cut(s) 490
TaqI TCGA 2 cut(s) 425, 431
TaqII GACCGA 1 cut(s) 84
TasI AATT 2 cut(s) 138, 505
TauI GCSGC 2 cut(s) 256, 412
Tru1I TTAA 2 cut(s) 317, 517
Tru9I TTAA 2 cut(s) 317, 517
TscAI CASTG 4 cut(s) 178, 204, 214, 495
TseFI GTSAC 2 cut(s) 205, 304
TseI GCWGC 1 cut(s) 109
Tsp45I GTSAC 2 cut(s) 205, 304
TspDTI ATGAA 4 cut(s) 75, 347, 386, 509
TspGWI ACGGA 1 cut(s) 166
TspRI CASTG 4 cut(s) 178, 204, 214, 495
VneI GTGCAC 1 cut(s) 44
VpaK11BI GGWCC 1 cut(s) 162
XceI RCATGY 1 cut(s) 260
XmiI GTMKAC 1 cut(s) 448
Zsp2I ATGCAT 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.