pycom06g14590

Rhodanese-like domain-containing protein 11

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0002465
Physical Location & Seq
Forward (+)
19311143 .. 19313979
2837 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 891 bp
ATGGAAGCTCTGGGATTTCCTTCTCTCAATACTCACAGAATTTCGTGCTCCAGTTCTCAGAAACGAAGAGCCACCAAACCACCATGTCAAAGAACACATTTTTCAAGCATTTCACCTCCATCTTCCCTAAAACTCACACCCTCTCACAGAAGTTACAATTTTCGGAGGAGTGCCACTCGGATGCAAGCTGACAGTGAAGATTACGAGTTGAAGCAAATGAGGGATATGGCTGCAGCCAAAAAGAGATGGGAAGCTCTGGTTAGGGAAGGAAAGGTTAAAGCTTTAACACCAAGGGAAGCCGGCTATGCAATTCAGCTATCTAACAAAACCCTTCTTGATGTTCGTCCCTCTACAGAACACAAAAAGGCATGGGTTAAAGGATCAACCTGGATTCCAATATTTGACGTTGGTAATGAATTCGACGCTGGAACTCTTTCCAGAAAGTTCATGAATTTCACAATGGGGGGTTGGTGGAGTGGCGTTCCTATGTTGTCGTATAACACCCAGTTCGTGGACAAAGTTAGTGAGAAGTTTCCAGAAGATACAGATCTTATTGTTGCATGTCAGAAGGGATTGAGATCTCTGGCCGCTTGTGAGCAGTTGTACAACGCAGGCTACAAAAACCTTTTCTGGGTTCAAGGAGGTTTAGAGGCTGCTGAAGAAGAGGATCTTGTCAGAGAAGGACCTCAGCCATTGAAGTTTGCTGGAATTGGTGGGGTTTCAGAATTCCTCGGTTGGACGGATCAGCAAAGAGCTGCAGCTGCCAAAGAAGGTTGGAGTTACCGATTAGTGTTCTCTGCCCGGCTGGTCGGAGTCATTGTCCTTGCCGATGCCTTGTTTTTTGGTGCTCAGCAATTCGGACATTATCTTCAGGATATAAGGTCGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhodanese

Protein Analysis

297

Amino Acids

33.27

Weight (kDa)

9.28

Isoelectric Point (pI)

46.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhodanese PF00581 108 - 219 1.4e-09 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 511
AciI CCGC 1 cut(s) 588
AclWI GGATC 3 cut(s) 388, 675, 750
AcoI YGGCCR 1 cut(s) 585
AcsI RAATTY 4 cut(s) 39, 416, 451, 725
AcuI CTGAAG 2 cut(s) 678, 854
AfaI GTAC 1 cut(s) 605
AfiI CCNNNNNNNGG 2 cut(s) 511, 631
AgsI TTSAA 4 cut(s) 105, 211, 638, 697
AjnI CCWGG 1 cut(s) 386
AluBI AGCT 7 cut(s) 8, 188, 254, 281, 316, 755, 761
AluI AGCT 7 cut(s) 8, 188, 254, 281, 316, 755, 761
Alw21I GWGCWC 2 cut(s) 50, 850
AlwI GGATC 3 cut(s) 388, 675, 750
AoxI GGCC 1 cut(s) 585
ApeKI GCWGC 6 cut(s) 230, 233, 653, 755, 758, 761
ApoI RAATTY 4 cut(s) 39, 416, 451, 725
Asp700I GAANNNNTTC 1 cut(s) 433
AspS9I GGNCC 1 cut(s) 683
AsuC2I CCSGG 1 cut(s) 802
AsuHPI GGTGA 1 cut(s) 105
AvaII GGWCC 1 cut(s) 683
Bbv12I GWGCWC 2 cut(s) 50, 850
BbvCI CCTCAGC 1 cut(s) 687
BbvI GCAGC 6 cut(s) 217, 245, 640, 742, 748, 770
BccI CCATC 2 cut(s) 127, 240
BciT130I CCWGG 1 cut(s) 388
BcnI CCSGG 1 cut(s) 802
BfmI CTRYAG 3 cut(s) 231, 351, 756
BglII AGATCT 2 cut(s) 547, 578
BisI GCNGC 7 cut(s) 231, 234, 588, 654, 756, 759, 762
BlpI GCTNAGC 1 cut(s) 849
BlsI GCNGC 7 cut(s) 232, 235, 589, 655, 757, 760, 763
Bme1390I CCNGG 2 cut(s) 388, 802
Bme18I GGWCC 1 cut(s) 683
BmgT120I GGNCC 1 cut(s) 683
BmrFI CCNGG 2 cut(s) 388, 802
BmrI ACTGGG 1 cut(s) 499
BmsI GCATC 2 cut(s) 171, 820
BmuI ACTGGG 1 cut(s) 499
BplI GAGNNNNNCTC 2 cut(s) 160, 192
BpmI CTGGAG 1 cut(s) 34
Bpu10I CCTNAGC 1 cut(s) 687
Bpu1102I GCTNAGC 1 cut(s) 849
BpuMI CCSGG 1 cut(s) 802
BsaBI GATNNNNATC 2 cut(s) 546, 577
BsaJI CCNNGG 2 cut(s) 290, 730
BsaXI ACNNNNNCTCC 2 cut(s) 804, 834
Bsc4I CCNNNNNNNGG 2 cut(s) 511, 631
Bse118I RCCGGY 1 cut(s) 299
Bse1I ACTGG 2 cut(s) 51, 505
Bse8I GATNNNNATC 2 cut(s) 546, 577
BseBI CCWGG 1 cut(s) 388
BseDI CCNNGG 2 cut(s) 290, 730
BseGI GGATG 1 cut(s) 186
BseJI GATNNNNATC 2 cut(s) 546, 577
BseLI CCNNNNNNNGG 2 cut(s) 511, 631
BseMII CTCAG 3 cut(s) 71, 701, 863
BseNI ACTGG 2 cut(s) 51, 505
BseRI GAGGAG 1 cut(s) 181
BseXI GCAGC 6 cut(s) 217, 245, 640, 742, 748, 770
BshFI GGCC 1 cut(s) 587
BsiHKAI GWGCWC 2 cut(s) 50, 850
BsiSI CCGG 2 cut(s) 300, 802
BslFI GGGAC 1 cut(s) 330
BslI CCNNNNNNNGG 2 cut(s) 511, 631
BsmFI GGGAC 1 cut(s) 330
BsnI GGCC 1 cut(s) 587
Bsp1286I GDGCHC 2 cut(s) 50, 850
Bsp1407I TGTACA 1 cut(s) 603
Bsp143I GATC 5 cut(s) 380, 547, 578, 667, 742
Bsp1720I GCTNAGC 1 cut(s) 849
BspACI CCGC 1 cut(s) 588
BspANI GGCC 1 cut(s) 587
BspCNI CTCAG 3 cut(s) 70, 700, 862
BspHI TCATGA 1 cut(s) 447
BspMAI CTGCAG 2 cut(s) 235, 760
BspPI GGATC 3 cut(s) 388, 675, 750
BspQI GCTCTTC 1 cut(s) 61
BsrFI RCCGGY 1 cut(s) 299
BsrGI TGTACA 1 cut(s) 603
BsrI ACTGG 2 cut(s) 51, 505
BssAI RCCGGY 1 cut(s) 299
BssECI CCNNGG 2 cut(s) 290, 730
BssMI GATC 5 cut(s) 380, 547, 578, 667, 742
BssT1I CCWWGG 1 cut(s) 290
Bst2UI CCWGG 1 cut(s) 388
Bst4CI ACNGT 1 cut(s) 194
Bst6I CTCTTC 2 cut(s) 61, 657
BstAUI TGTACA 1 cut(s) 603
BstC8I GCNNGC 3 cut(s) 186, 301, 613
BstDEI CTNAG 3 cut(s) 57, 687, 849
BstF5I GGATG 1 cut(s) 186
BstKTI GATC 5 cut(s) 383, 550, 581, 670, 745
BstMBI GATC 5 cut(s) 380, 547, 578, 667, 742
BstMWI GCNNNNNNNGC 2 cut(s) 305, 761
BstNI CCWGG 1 cut(s) 388
BstNSI RCATGY 1 cut(s) 564
BstSCI CCNGG 2 cut(s) 386, 800
BstSFI CTRYAG 3 cut(s) 231, 351, 756
BstV1I GCAGC 6 cut(s) 217, 245, 640, 742, 748, 770
BstX2I RGATCY 3 cut(s) 547, 578, 667
BstYI RGATCY 3 cut(s) 547, 578, 667
BsuRI GGCC 1 cut(s) 587
BtsCI GGATG 1 cut(s) 186
BtsIMutI CAGTG 2 cut(s) 199, 886
Cac8I GCNNGC 3 cut(s) 186, 301, 613
CciI TCATGA 1 cut(s) 447
Cfr10I RCCGGY 1 cut(s) 299
Cfr13I GGNCC 1 cut(s) 683
CseI GACGC 1 cut(s) 431
Csp6I GTAC 1 cut(s) 604
CviAII CATG 4 cut(s) 84, 369, 448, 561
CviQI GTAC 1 cut(s) 604
DdeI CTNAG 3 cut(s) 57, 687, 849
DpnI GATC 5 cut(s) 382, 549, 580, 669, 744
DpnII GATC 5 cut(s) 380, 547, 578, 667, 742
EaeI YGGCCR 1 cut(s) 585
Eam1104I CTCTTC 2 cut(s) 61, 657
EarI CTCTTC 2 cut(s) 61, 657
Eco130I CCWWGG 1 cut(s) 290
Eco47I GGWCC 1 cut(s) 683
Eco57I CTGAAG 2 cut(s) 678, 854
EcoO109I RGGNCCY 1 cut(s) 683
EcoRI GAATTC 2 cut(s) 416, 725
EcoRII CCWGG 1 cut(s) 386
EcoT14I CCWWGG 1 cut(s) 290
ErhI CCWWGG 1 cut(s) 290
FaeI CATG 4 cut(s) 87, 372, 451, 564
FaiI YATR 9 cut(s) 85, 227, 306, 370, 449, 488, 498, 562, 878
FalI AAGNNNNNCTT 2 cut(s) 654, 686
FaqI GGGAC 1 cut(s) 330
FatI CATG 4 cut(s) 83, 368, 447, 560
Fnu4HI GCNGC 7 cut(s) 231, 234, 588, 654, 756, 759, 762
FokI GGATG 1 cut(s) 193
Fsp4HI GCNGC 7 cut(s) 231, 234, 588, 654, 756, 759, 762
GluI GCNGC 7 cut(s) 231, 234, 588, 654, 756, 759, 762
GsuI CTGGAG 1 cut(s) 34
HaeIII GGCC 1 cut(s) 587
HapII CCGG 2 cut(s) 300, 802
HgaI GACGC 1 cut(s) 431
Hin1II CATG 4 cut(s) 87, 372, 451, 564
HindIII AAGCTT 1 cut(s) 279
HinfI GANTC 2 cut(s) 391, 813
HpaII CCGG 2 cut(s) 300, 802
HphI GGTGA 1 cut(s) 105
Hpy166II GTNNAC 1 cut(s) 514
Hpy188I TCNGA 8 cut(s) 60, 165, 180, 567, 677, 724, 812, 860
Hpy188III TCNNGA 5 cut(s) 335, 438, 448, 536, 872
Hpy8I GTNNAC 1 cut(s) 514
Hpy99I CGWCG 1 cut(s) 425
HpyAV CCTTC 6 cut(s) 30, 260, 341, 562, 674, 764
HpyCH4III ACNGT 1 cut(s) 194
HpyCH4IV ACGT 1 cut(s) 405
HpyCH4V TGCA 5 cut(s) 184, 233, 308, 560, 758
HpyF10VI GCNNNNNNNGC 2 cut(s) 305, 761
HpyF3I CTNAG 3 cut(s) 57, 687, 849
HpySE526I ACGT 1 cut(s) 405
Hsp92II CATG 4 cut(s) 87, 372, 451, 564
KroI GCCGGC 1 cut(s) 299
KroNI GCCGGC 1 cut(s) 301
Kzo9I GATC 5 cut(s) 380, 547, 578, 667, 742
LguI GCTCTTC 1 cut(s) 61
LmnI GCTCC 1 cut(s) 53
Lsp1109I GCAGC 6 cut(s) 217, 245, 640, 742, 748, 770
LweI GCATC 2 cut(s) 171, 820
MaeII ACGT 1 cut(s) 405
MaeIII GTNAC 2 cut(s) 152, 779
MalI GATC 5 cut(s) 382, 549, 580, 669, 744
MboI GATC 5 cut(s) 380, 547, 578, 667, 742
MboII GAAGA 7 cut(s) 78, 114, 209, 551, 671, 674, 860
MflI RGATCY 3 cut(s) 547, 578, 667
MhlI GDGCHC 2 cut(s) 50, 850
MluCI AATT 8 cut(s) 39, 157, 309, 416, 451, 708, 725, 854
MlyI GAGTC 1 cut(s) 822
MmeI TCCRAC 3 cut(s) 716, 755, 790
MroNI GCCGGC 1 cut(s) 299
MroXI GAANNNNTTC 1 cut(s) 433
MseI TTAA 3 cut(s) 276, 284, 375
MslI CAYNNNNRTG 1 cut(s) 179
MspA1I CMGCKG 1 cut(s) 761
MspI CCGG 2 cut(s) 300, 802
MspR9I CCNGG 2 cut(s) 388, 802
MvaI CCWGG 1 cut(s) 388
MwoI GCNNNNNNNGC 2 cut(s) 305, 761
NaeI GCCGGC 1 cut(s) 301
NciI CCSGG 1 cut(s) 802
NdeII GATC 5 cut(s) 380, 547, 578, 667, 742
NgoMIV GCCGGC 1 cut(s) 299
NlaIII CATG 4 cut(s) 87, 372, 451, 564
NspI RCATGY 1 cut(s) 564
PagI TCATGA 1 cut(s) 447
PciSI GCTCTTC 1 cut(s) 61
PdiI GCCGGC 1 cut(s) 301
PdmI GAANNNNTTC 1 cut(s) 433
PfeI GAWTC 1 cut(s) 391
PflMI CCANNNNNTGG 1 cut(s) 511
PkrI GCNGC 7 cut(s) 232, 235, 589, 655, 757, 760, 763
PleI GAGTC 1 cut(s) 821
PpsI GAGTC 1 cut(s) 821
PpuMI RGGWCCY 1 cut(s) 683
Psp5II RGGWCCY 1 cut(s) 683
Psp6I CCWGG 1 cut(s) 386
PspGI CCWGG 1 cut(s) 386
PspPI GGNCC 1 cut(s) 683
PspPPI RGGWCCY 1 cut(s) 683
PstI CTGCAG 2 cut(s) 235, 760
PsuI RGATCY 3 cut(s) 547, 578, 667
PvuII CAGCTG 1 cut(s) 761
RsaI GTAC 1 cut(s) 605
RsaNI GTAC 1 cut(s) 604
RseI CAYNNNNRTG 1 cut(s) 179
SapI GCTCTTC 1 cut(s) 61
SaqAI TTAA 3 cut(s) 276, 284, 375
SatI GCNGC 7 cut(s) 231, 234, 588, 654, 756, 759, 762
Sau3AI GATC 5 cut(s) 380, 547, 578, 667, 742
Sau96I GGNCC 1 cut(s) 683
SchI GAGTC 1 cut(s) 822
ScrFI CCNGG 2 cut(s) 388, 802
SduI GDGCHC 2 cut(s) 50, 850
SfaNI GCATC 2 cut(s) 171, 820
SfcI CTRYAG 3 cut(s) 231, 351, 756
SinI GGWCC 1 cut(s) 683
SmiMI CAYNNNNRTG 1 cut(s) 179
Sse9I AATT 8 cut(s) 39, 157, 309, 416, 451, 708, 725, 854
SsiI CCGC 1 cut(s) 588
SspI AATATT 1 cut(s) 399
StyD4I CCNGG 2 cut(s) 386, 800
StyI CCWWGG 1 cut(s) 290
TaaI ACNGT 1 cut(s) 194
TaiI ACGT 1 cut(s) 408
TaqI TCGA 1 cut(s) 420
TasI AATT 8 cut(s) 39, 157, 309, 416, 451, 708, 725, 854
TatI WGTACW 1 cut(s) 603
TauI GCSGC 1 cut(s) 590
TfiI GAWTC 1 cut(s) 391
Tru1I TTAA 3 cut(s) 276, 284, 375
Tru9I TTAA 3 cut(s) 276, 284, 375
TscAI CASTG 1 cut(s) 199
TseI GCWGC 6 cut(s) 230, 233, 653, 755, 758, 761
TspDTI ATGAA 3 cut(s) 429, 436, 464
TspGWI ACGGA 1 cut(s) 755
TspRI CASTG 1 cut(s) 199
Van91I CCANNNNNTGG 1 cut(s) 511
VpaK11BI GGWCC 1 cut(s) 683
XapI RAATTY 4 cut(s) 39, 416, 451, 725
XceI RCATGY 1 cut(s) 564
XmnI GAANNNNTTC 1 cut(s) 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.