pycom07g07980

At1g28695-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0002782
Physical Location & Seq
Forward (+)
7381260 .. 7386407
5148 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1119 bp
ATGGATCACCCAAAACATTCCTCTCTCGGATCCAATCTTGCCTTCCTCGCTCTATTCTTGGCTTGCGTTGTTTATGTTTGCATATGGTCGTCTTCTTCCTCTCTAATAAACCCTCTCTTTCCCTTTCAGAAACGTGGCGTCGACTGTGCGTCGCAAACAACCACAGCCGCCGCCACCACGGAATTTAATGTGGTGGAGGACGAGCTTGAGACCGTCTTGGGCAAGGCCTCCATGGATAACAAGACCGTGATCATTGCCGTTATCAACACGGCATATGCAGTCCAAGATGTGAAGGCAGACACCACCATGCTTGACCTTTTTATTGAGAGCTTTTGGCAAGGGGAGGGCACGAGGACGTTGGTCGACCACCTCGTCCTCGTTGCGGTTGATCAAACCGCCTTCGACCGGTGCATGTTTCTGCGGCTGAATTGCTACCGGTTGGAGACGGAGGGTGTGGATTTTGGGGGAGAGAAGCTTTTCATGTCTGAAGATTTTATCAAGATGATGTGGAGGAGAACTTGGTTACTGTTGGAGGTTCTCAAGCGTGGCTACAGCTTCGTGTTTACGACCGATGTTGTGTGGCTAAGGGACCCCTTCACAAAACTAAGCAAGAACGAAACAGAAGATTTACAAATGAGTACAGATATGTTCTTCGGGGATCGATCTTGGAACGCAAGTCAGTTGATCAACACTGGCTTCTACTACGTCAGATCCAATAACAAGACCATAGCACTGTTTGATAGATGGTACACCTCCAAGGACAATGCCACAGGACTGAAAGAGCAGGATGTCCTATTGAGCTTGTTCCGAGGGGGTATTACCGGGGAGTTGGGACTTAGGGTAAGGTTCTTAGACACCCTTTATTTCAGTGGTTTTTGCCAAAACAGCAAGGACTTCGGGCAAGTGACCACGGTCCACGCCAATTGTTGCCGGAGTATTGTCGCCAAAGTTAAGGACTTGAAAGCCGTCCTCCGGGATTGGAAGCGGTTCAAGAGGTTCATGTCCCACAAGAGGAGTTCTGGTACTAGTACGACGAGTTTCCAATGGACTGGGCACTTTGGATGTTGGAATTCCTGGGCCGTTCCCCGTAACTCTGGTAATGCAACTCATAAGACATGA

Protein Analysis

373

Amino Acids

42.37

Weight (kDa)

8.3

Isoelectric Point (pI)

38.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nucleotid_trans PF03407 120 - 320 8.4e-58 Nucleotide-diphospho-sugar transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 371
AccI GTMKAC 2 cut(s) 141, 363
AciI CCGC 6 cut(s) 168, 171, 383, 396, 421, 985
AclWI GGATC 5 cut(s) 12, 24, 37, 666, 705
AcsI RAATTY 2 cut(s) 182, 1069
AcuI CTGAAG 1 cut(s) 507
AcyI GRCGYC 1 cut(s) 138
AfaI GTAC 4 cut(s) 640, 749, 1024, 1030
AfiI CCNNNNNNNGG 4 cut(s) 382, 405, 972, 1011
AgeI ACCGGT 2 cut(s) 405, 435
AgsI TTSAA 2 cut(s) 961, 991
AhdI GACNNNNNGTC 1 cut(s) 148
AhlI ACTAGT 1 cut(s) 1025
AjnI CCWGG 1 cut(s) 1073
AjuI GAANNNNNNNTTGG 2 cut(s) 26, 58
AluBI AGCT 5 cut(s) 205, 330, 475, 555, 801
AluI AGCT 5 cut(s) 205, 330, 475, 555, 801
Alw26I GTCTC 2 cut(s) 203, 437
AlwI GGATC 5 cut(s) 12, 24, 37, 666, 705
AoxI GGCC 2 cut(s) 225, 1077
ApoI RAATTY 2 cut(s) 182, 1069
AsiGI ACCGGT 2 cut(s) 405, 435
Asp700I GAANNNNTTC 2 cut(s) 476, 986
AspS9I GGNCC 3 cut(s) 589, 913, 1077
AsuC2I CCSGG 2 cut(s) 823, 974
AvaII GGWCC 2 cut(s) 589, 913
BaeGI GKGCMC 2 cut(s) 350, 1056
BamHI GGATCC 1 cut(s) 29
BauI CACGAG 1 cut(s) 349
BbsI GAAGAC 1 cut(s) 84
BccI CCATC 1 cut(s) 738
BceAI ACGGC 4 cut(s) 242, 285, 950, 1064
BcgI CGANNNNNNTGC 2 cut(s) 877, 911
BciT130I CCWGG 1 cut(s) 1075
BclI TGATCA 3 cut(s) 249, 388, 684
BcnI CCSGG 2 cut(s) 823, 974
BcoDI GTCTC 2 cut(s) 203, 437
BcuI ACTAGT 1 cut(s) 1025
BfaI CTAG 1 cut(s) 1026
BfmI CTRYAG 1 cut(s) 550
BisI GCNGC 3 cut(s) 168, 171, 422
BlsI GCNGC 3 cut(s) 169, 172, 423
Bme1390I CCNGG 3 cut(s) 823, 974, 1075
Bme18I GGWCC 2 cut(s) 589, 913
BmeRI GACNNNNNGTC 1 cut(s) 148
BmgT120I GGNCC 3 cut(s) 589, 913, 1077
BmiI GGNNCC 3 cut(s) 31, 590, 591
BmrFI CCNGG 3 cut(s) 823, 974, 1075
BmrI ACTGGG 1 cut(s) 1059
BmuI ACTGGG 1 cut(s) 1059
BoxI GACNNNNGTC 2 cut(s) 359, 911
BpiI GAAGAC 1 cut(s) 84
Bpu10I CCTNAGC 1 cut(s) 584
BpuEI CTTGAG 2 cut(s) 227, 524
BpuMI CCSGG 2 cut(s) 823, 974
Bsa29I ATCGAT 1 cut(s) 661
BsaHI GRCGYC 1 cut(s) 138
BsaI GGTCTC 1 cut(s) 203
BsaJI CCNNGG 7 cut(s) 177, 231, 756, 808, 822, 909, 1074
BsaWI WCCGGW 2 cut(s) 405, 435
BsaXI ACNNNNNCTCC 2 cut(s) 1006, 1036
Bsc4I CCNNNNNNNGG 4 cut(s) 382, 405, 972, 1011
Bse118I RCCGGY 2 cut(s) 405, 435
Bse1I ACTGG 2 cut(s) 697, 1054
Bse3DI GCAATG 1 cut(s) 252
BseBI CCWGG 1 cut(s) 1075
BseCI ATCGAT 1 cut(s) 661
BseDI CCNNGG 7 cut(s) 177, 231, 756, 808, 822, 909, 1074
BseGI GGATG 2 cut(s) 793, 1067
BseLI CCNNNNNNNGG 4 cut(s) 382, 405, 972, 1011
BseMI GCAATG 1 cut(s) 252
BseNI ACTGG 2 cut(s) 697, 1054
BseRI GAGGAG 2 cut(s) 526, 1027
BseSI GKGCMC 2 cut(s) 350, 1056
Bsh1285I CGRYCG 2 cut(s) 406, 570
BshFI GGCC 2 cut(s) 227, 1079
BshTI ACCGGT 2 cut(s) 405, 435
BshVI ATCGAT 1 cut(s) 661
BsiEI CGRYCG 2 cut(s) 406, 570
BsiSI CCGG 5 cut(s) 406, 436, 822, 931, 973
BslFI GGGAC 3 cut(s) 602, 846, 988
BslI CCNNNNNNNGG 4 cut(s) 382, 405, 972, 1011
BsmAI GTCTC 2 cut(s) 203, 437
BsmBI CGTCTC 1 cut(s) 437
BsmFI GGGAC 3 cut(s) 602, 846, 988
BsnI GGCC 2 cut(s) 227, 1079
Bso31I GGTCTC 1 cut(s) 203
Bsp1286I GDGCHC 2 cut(s) 350, 1056
Bsp143I GATC 8 cut(s) 4, 29, 249, 388, 658, 662, 684, 710
Bsp19I CCATGG 1 cut(s) 231
BspACI CCGC 6 cut(s) 168, 171, 383, 396, 421, 985
BspANI GGCC 2 cut(s) 227, 1079
BspDI ATCGAT 1 cut(s) 661
BspLI GGNNCC 3 cut(s) 31, 590, 591
BspPI GGATC 5 cut(s) 12, 24, 37, 666, 705
BspTNI GGTCTC 1 cut(s) 203
BsrDI GCAATG 1 cut(s) 252
BsrFI RCCGGY 2 cut(s) 405, 435
BsrI ACTGG 2 cut(s) 697, 1054
BssAI RCCGGY 2 cut(s) 405, 435
BssECI CCNNGG 7 cut(s) 177, 231, 756, 808, 822, 909, 1074
BssMI GATC 8 cut(s) 4, 29, 249, 388, 658, 662, 684, 710
BssNI GRCGYC 1 cut(s) 138
BssSI CACGAG 1 cut(s) 349
BssT1I CCWWGG 2 cut(s) 231, 756
Bst2BI CACGAG 1 cut(s) 349
Bst2UI CCWGG 1 cut(s) 1075
Bst4CI ACNGT 6 cut(s) 146, 214, 247, 528, 735, 913
BstACI GRCGYC 1 cut(s) 138
BstC8I GCNNGC 1 cut(s) 64
BstDEI CTNAG 4 cut(s) 584, 605, 836, 850
BstDSI CCRYGG 3 cut(s) 177, 231, 909
BstF5I GGATG 2 cut(s) 793, 1067
BstKTI GATC 8 cut(s) 7, 32, 252, 391, 661, 665, 687, 713
BstMAI GTCTC 2 cut(s) 203, 437
BstMBI GATC 8 cut(s) 4, 29, 249, 388, 658, 662, 684, 710
BstMCI CGRYCG 2 cut(s) 406, 570
BstMWI GCNNNNNNNGC 2 cut(s) 47, 885
BstNI CCWGG 1 cut(s) 1075
BstNSI RCATGY 1 cut(s) 415
BstPAI GACNNNNGTC 2 cut(s) 359, 911
BstSCI CCNGG 3 cut(s) 821, 972, 1073
BstSFI CTRYAG 1 cut(s) 550
BstSLI GKGCMC 2 cut(s) 350, 1056
BstV2I GAAGAC 1 cut(s) 84
BstX2I RGATCY 2 cut(s) 29, 710
BstXI CCANNNNNNTGG 1 cut(s) 1049
BstYI RGATCY 2 cut(s) 29, 710
Bsu15I ATCGAT 1 cut(s) 661
BsuRI GGCC 2 cut(s) 227, 1079
BsuTUI ATCGAT 1 cut(s) 661
BtgI CCRYGG 3 cut(s) 177, 231, 909
BtsCI GGATG 2 cut(s) 793, 1067
BtsIMutI CAGTG 3 cut(s) 690, 731, 874
Cac8I GCNNGC 1 cut(s) 64
Cfr10I RCCGGY 2 cut(s) 405, 435
Cfr13I GGNCC 3 cut(s) 589, 913, 1077
ClaI ATCGAT 1 cut(s) 661
CseI GACGC 2 cut(s) 127, 138
Csp6I GTAC 4 cut(s) 639, 748, 1023, 1029
CspAI ACCGGT 2 cut(s) 405, 435
CspCI CAANNNNNGTGG 4 cut(s) 292, 327, 905, 940
CviAII CATG 6 cut(s) 232, 307, 412, 481, 1000, 1116
CviQI GTAC 4 cut(s) 639, 748, 1023, 1029
DdeI CTNAG 4 cut(s) 584, 605, 836, 850
DpnI GATC 8 cut(s) 6, 31, 251, 390, 660, 664, 686, 712
DpnII GATC 8 cut(s) 4, 29, 249, 388, 658, 662, 684, 710
DrdI GACNNNNNNGTC 1 cut(s) 371
DriI GACNNNNNGTC 1 cut(s) 148
DseDI GACNNNNNNGTC 1 cut(s) 371
Eam1105I GACNNNNNGTC 1 cut(s) 148
Eco130I CCWWGG 2 cut(s) 231, 756
Eco147I AGGCCT 1 cut(s) 227
Eco31I GGTCTC 1 cut(s) 203
Eco47I GGWCC 2 cut(s) 589, 913
Eco57I CTGAAG 1 cut(s) 507
EcoO109I RGGNCCY 1 cut(s) 589
EcoRI GAATTC 1 cut(s) 1069
EcoRII CCWGG 1 cut(s) 1073
EcoT14I CCWWGG 2 cut(s) 231, 756
ErhI CCWWGG 2 cut(s) 231, 756
Esp3I CGTCTC 1 cut(s) 437
FaeI CATG 6 cut(s) 235, 310, 415, 484, 1003, 1119
FaqI GGGAC 3 cut(s) 602, 846, 988
FatI CATG 6 cut(s) 231, 306, 411, 480, 999, 1115
FauNDI CATATG 2 cut(s) 83, 274
FbaI TGATCA 3 cut(s) 249, 388, 684
FblI GTMKAC 2 cut(s) 141, 363
Fnu4HI GCNGC 3 cut(s) 168, 171, 422
FokI GGATG 2 cut(s) 800, 1074
Fsp4HI GCNGC 3 cut(s) 168, 171, 422
FspBI CTAG 1 cut(s) 1026
GluI GCNGC 3 cut(s) 168, 171, 422
HaeIII GGCC 2 cut(s) 227, 1079
HapII CCGG 5 cut(s) 406, 436, 822, 931, 973
HgaI GACGC 2 cut(s) 127, 138
Hin1I GRCGYC 1 cut(s) 138
Hin1II CATG 6 cut(s) 235, 310, 415, 484, 1003, 1119
HincII GTYRAC 2 cut(s) 142, 364
HindII GTYRAC 2 cut(s) 142, 364
HindIII AAGCTT 1 cut(s) 473
HpaII CCGG 5 cut(s) 406, 436, 822, 931, 973
Hpy166II GTNNAC 5 cut(s) 142, 364, 564, 750, 916
Hpy188I TCNGA 5 cut(s) 29, 129, 487, 710, 809
Hpy188III TCNNGA 2 cut(s) 499, 991
Hpy8I GTNNAC 5 cut(s) 142, 364, 564, 750, 916
Hpy99I CGWCG 3 cut(s) 143, 154, 1036
HpyAV CCTTC 4 cut(s) 52, 286, 409, 604
HpyCH4III ACNGT 6 cut(s) 146, 214, 247, 528, 735, 913
HpyCH4IV ACGT 3 cut(s) 133, 356, 705
HpyCH4V TGCA 4 cut(s) 81, 278, 411, 1103
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 885
HpyF3I CTNAG 4 cut(s) 584, 605, 836, 850
HpySE526I ACGT 3 cut(s) 133, 356, 705
Hsp92I GRCGYC 1 cut(s) 138
Hsp92II CATG 6 cut(s) 235, 310, 415, 484, 1003, 1119
KflI GGGWCCC 1 cut(s) 589
Ksp22I TGATCA 3 cut(s) 249, 388, 684
Kzo9I GATC 8 cut(s) 4, 29, 249, 388, 658, 662, 684, 710
MaeI CTAG 1 cut(s) 1026
MaeII ACGT 3 cut(s) 133, 356, 705
MaeIII GTNAC 3 cut(s) 522, 904, 1088
MalI GATC 8 cut(s) 6, 31, 251, 390, 660, 664, 686, 712
MboI GATC 8 cut(s) 4, 29, 249, 388, 658, 662, 684, 710
MboII GAAGA 5 cut(s) 84, 87, 500, 635, 643
MfeI CAATTG 1 cut(s) 922
MflI RGATCY 2 cut(s) 29, 710
MhlI GDGCHC 2 cut(s) 350, 1056
MluCI AATT 4 cut(s) 182, 427, 922, 1069
MmeI TCCRAC 3 cut(s) 420, 510, 1046
MroXI GAANNNNTTC 2 cut(s) 476, 986
MseI TTAA 2 cut(s) 186, 951
MslI CAYNNNNRTG 1 cut(s) 305
MspI CCGG 5 cut(s) 406, 436, 822, 931, 973
MspR9I CCNGG 3 cut(s) 823, 974, 1075
MunI CAATTG 1 cut(s) 922
MvaI CCWGG 1 cut(s) 1075
MwoI GCNNNNNNNGC 2 cut(s) 47, 885
NciI CCSGG 2 cut(s) 823, 974
NcoI CCATGG 1 cut(s) 231
NdeI CATATG 2 cut(s) 83, 274
NdeII GATC 8 cut(s) 4, 29, 249, 388, 658, 662, 684, 710
NlaIII CATG 6 cut(s) 235, 310, 415, 484, 1003, 1119
NlaIV GGNNCC 3 cut(s) 31, 590, 591
NmuCI GTSAC 1 cut(s) 904
NspI RCATGY 1 cut(s) 415
PceI AGGCCT 1 cut(s) 227
PcsI WCGNNNNNNNCGW 1 cut(s) 369
PdmI GAANNNNTTC 2 cut(s) 476, 986
PfoI TCCNGGA 1 cut(s) 972
PinAI ACCGGT 2 cut(s) 405, 435
PkrI GCNGC 3 cut(s) 169, 172, 423
PpuMI RGGWCCY 1 cut(s) 589
PshAI GACNNNNGTC 2 cut(s) 359, 911
Psp5II RGGWCCY 1 cut(s) 589
Psp6I CCWGG 1 cut(s) 1073
PspGI CCWGG 1 cut(s) 1073
PspN4I GGNNCC 3 cut(s) 31, 590, 591
PspPI GGNCC 3 cut(s) 589, 913, 1077
PspPPI RGGWCCY 1 cut(s) 589
PsuI RGATCY 2 cut(s) 29, 710
RsaI GTAC 4 cut(s) 640, 749, 1024, 1030
RsaNI GTAC 4 cut(s) 639, 748, 1023, 1029
RseI CAYNNNNRTG 1 cut(s) 305
SalI GTCGAC 2 cut(s) 140, 362
SaqAI TTAA 2 cut(s) 186, 951
SatI GCNGC 3 cut(s) 168, 171, 422
Sau3AI GATC 8 cut(s) 4, 29, 249, 388, 658, 662, 684, 710
Sau96I GGNCC 3 cut(s) 589, 913, 1077
ScrFI CCNGG 3 cut(s) 823, 974, 1075
SduI GDGCHC 2 cut(s) 350, 1056
SfcI CTRYAG 1 cut(s) 550
SinI GGWCC 2 cut(s) 589, 913
SmiMI CAYNNNNRTG 1 cut(s) 305
SmlI CTYRAG 2 cut(s) 206, 539
SmoI CTYRAG 2 cut(s) 206, 539
SpeI ACTAGT 1 cut(s) 1025
Sse9I AATT 4 cut(s) 182, 427, 922, 1069
SseBI AGGCCT 1 cut(s) 227
SsiI CCGC 6 cut(s) 168, 171, 383, 396, 421, 985
SspMI CTAG 1 cut(s) 1026
StuI AGGCCT 1 cut(s) 227
StyD4I CCNGG 3 cut(s) 821, 972, 1073
StyI CCWWGG 2 cut(s) 231, 756
TaaI ACNGT 6 cut(s) 146, 214, 247, 528, 735, 913
TaiI ACGT 3 cut(s) 136, 359, 708
TaqI TCGA 4 cut(s) 141, 363, 402, 661
TaqII GACCGA 1 cut(s) 584
TasI AATT 4 cut(s) 182, 427, 922, 1069
TatI WGTACW 1 cut(s) 638
TauI GCSGC 3 cut(s) 170, 173, 424
Tru1I TTAA 2 cut(s) 186, 951
Tru9I TTAA 2 cut(s) 186, 951
TscAI CASTG 3 cut(s) 697, 738, 874
TseFI GTSAC 1 cut(s) 904
Tsp45I GTSAC 1 cut(s) 904
TspDTI ATGAA 2 cut(s) 469, 988
TspGWI ACGGA 2 cut(s) 194, 461
TspRI CASTG 3 cut(s) 697, 738, 874
VpaK11BI GGWCC 2 cut(s) 589, 913
XapI RAATTY 2 cut(s) 182, 1069
XceI RCATGY 1 cut(s) 415
XmiI GTMKAC 2 cut(s) 141, 363
XmnI GAANNNNTTC 2 cut(s) 476, 986
XspI CTAG 1 cut(s) 1026
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.