pycom07g09110

motif in proteasome subunits, Int-6, Nip-1 and TRIP-15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0001916
Physical Location & Seq
Reverse (-)
8321561 .. 8325384
3824 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 744 bp
ATGGAGATTGAGCAAAAGCAAGAGGAGGTCATTGATCACTACGTGAAGCAAGCCTCATCACTAGATGGCTCCGCTCTCGGTCCTCTCGTCGCAGAAGTCACCTCCCACCCTGCCCTTTTTGCCTTTTCGGAAATTATCGCTGTCCCTAATGTTCTTCAGCTTGAAGGAACTGAAAATTCTGTGTACCTCGATGTGCTTCGATTGTTTGCACATGGCACATGGAGTGATTACAAGAGTGATGCCAGTCGTCGTCCACAACTGGTCCCTGATCAAGTCTTAAAGCTGAAACAGCTTACTGTTCTTACACTGGCTGAGACAAACAAGGTACTACCGTATCATCAACTTATGCAGGAGTTAGATGTTATCAATGTACGAGAGCTCGAGGATTTTCTCATTAATGAGTGCATGTATGTGGGCATACTAAGAGGAAAGCTGGACCAGTTGCGAAAATGCTTTGAGGTCCAGTTTGCTGCAGGGAGAGATCCGAGACCTGGACAACTTGGGAGTATGATTCAAACACTGTCAAACTGGTTGGATACCTCAAGCAATCTTCTTATCTCAATTCAAGAAAAGATGAAGTGGGCTGATACTATGACTGAAGTGGCCAAGGAACACAAGAAAGAAGTTGGAAATAGGGTGGAAGAAGTAAAGAAGTCGATTTCCATCAAGAAGTTCGCTACTGTAAGCAGGCCGACATTGACTTCCGGGGGCATGAGGAGATCTACTCCGAACCTGGAGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

248

Amino Acids

27.88

Weight (kDa)

5.66

Isoelectric Point (pI)

44.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCI PF01399 86 - 155 7.5e-06 PCI domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 74
AciI CCGC 1 cut(s) 72
AclWI GGATC 1 cut(s) 476
AcoI YGGCCR 1 cut(s) 603
AcsI RAATTY 1 cut(s) 175
AcuI CTGAAG 2 cut(s) 140, 618
AdeI CACNNNGTG 1 cut(s) 43
AfaI GTAC 3 cut(s) 185, 327, 372
AfiI CCNNNNNNNGG 1 cut(s) 491
AgsI TTSAA 3 cut(s) 164, 515, 566
AjnI CCWGG 2 cut(s) 490, 732
AluBI AGCT 5 cut(s) 160, 283, 292, 379, 433
AluI AGCT 5 cut(s) 160, 283, 292, 379, 433
Alw21I GWGCWC 1 cut(s) 381
Alw26I GTCTC 2 cut(s) 308, 481
AlwI GGATC 1 cut(s) 476
Ama87I CYCGRG 1 cut(s) 380
AoxI GGCC 2 cut(s) 603, 689
ApeKI GCWGC 1 cut(s) 470
ApoI RAATTY 1 cut(s) 175
AseI ATTAAT 1 cut(s) 396
AspS9I GGNCC 4 cut(s) 80, 262, 436, 460
AsuC2I CCSGG 1 cut(s) 706
AsuHPI GGTGA 1 cut(s) 91
AvaI CYCGRG 1 cut(s) 380
AvaII GGWCC 4 cut(s) 80, 262, 436, 460
BaeI ACNNNNGTAYC 4 cut(s) 317, 317, 350, 350
BalI TGGCCA 1 cut(s) 605
BanII GRGCYC 1 cut(s) 381
Bbv12I GWGCWC 1 cut(s) 381
BbvI GCAGC 1 cut(s) 457
BccI CCATC 2 cut(s) 59, 671
BciT130I CCWGG 2 cut(s) 492, 734
BciVI GTATCC 1 cut(s) 529
BclI TGATCA 2 cut(s) 34, 268
BcnI CCSGG 1 cut(s) 706
BcoDI GTCTC 2 cut(s) 308, 481
BfaI CTAG 1 cut(s) 62
BfmI CTRYAG 1 cut(s) 471
BfuI GTATCC 1 cut(s) 529
BglII AGATCT 1 cut(s) 719
BisI GCNGC 1 cut(s) 471
BlsI GCNGC 1 cut(s) 472
Bme1390I CCNGG 3 cut(s) 492, 706, 734
Bme18I GGWCC 4 cut(s) 80, 262, 436, 460
BmeT110I CYCGRG 1 cut(s) 380
BmgT120I GGNCC 4 cut(s) 80, 262, 436, 460
BmiI GGNNCC 2 cut(s) 70, 264
BmrFI CCNGG 3 cut(s) 492, 706, 734
BmsI GCATC 1 cut(s) 229
BplI GAGNNNNNCTC 2 cut(s) 709, 741
BpuEI CTTGAG 1 cut(s) 526
BpuMI CCSGG 1 cut(s) 706
BsaAI YACGTR 1 cut(s) 43
BsaBI GATNNNNATC 1 cut(s) 662
BsaI GGTCTC 1 cut(s) 481
BsaJI CCNNGG 2 cut(s) 606, 705
Bsc4I CCNNNNNNNGG 1 cut(s) 491
Bse1I ACTGG 6 cut(s) 243, 264, 312, 439, 463, 533
Bse8I GATNNNNATC 1 cut(s) 662
BseBI CCWGG 2 cut(s) 492, 734
BseDI CCNNGG 2 cut(s) 606, 705
BseJI GATNNNNATC 1 cut(s) 662
BseLI CCNNNNNNNGG 1 cut(s) 491
BseMII CTCAG 1 cut(s) 303
BseNI ACTGG 6 cut(s) 243, 264, 312, 439, 463, 533
BseRI GAGGAG 2 cut(s) 38, 730
BseXI GCAGC 1 cut(s) 457
BshFI GGCC 2 cut(s) 605, 691
BsiHKAI GWGCWC 1 cut(s) 381
BsiHKCI CYCGRG 1 cut(s) 380
BsiSI CCGG 1 cut(s) 705
BslFI GGGAC 2 cut(s) 128, 248
BslI CCNNNNNNNGG 1 cut(s) 491
BsmAI GTCTC 2 cut(s) 308, 481
BsmFI GGGAC 2 cut(s) 128, 248
BsnI GGCC 2 cut(s) 605, 691
Bso31I GGTCTC 1 cut(s) 481
BsoBI CYCGRG 1 cut(s) 380
Bsp1286I GDGCHC 1 cut(s) 381
Bsp143I GATC 4 cut(s) 34, 268, 481, 719
BspACI CCGC 1 cut(s) 72
BspANI GGCC 2 cut(s) 605, 691
BspCNI CTCAG 1 cut(s) 304
BspLI GGNNCC 2 cut(s) 70, 264
BspMAI CTGCAG 1 cut(s) 475
BspPI GGATC 1 cut(s) 476
BspTNI GGTCTC 1 cut(s) 481
BsrBI CCGCTC 1 cut(s) 74
BsrI ACTGG 6 cut(s) 243, 264, 312, 439, 463, 533
BssECI CCNNGG 2 cut(s) 606, 705
BssMI GATC 4 cut(s) 34, 268, 481, 719
BssT1I CCWWGG 1 cut(s) 606
Bst2UI CCWGG 2 cut(s) 492, 734
Bst4CI ACNGT 4 cut(s) 298, 333, 522, 682
BstBAI YACGTR 1 cut(s) 43
BstC8I GCNNGC 2 cut(s) 51, 689
BstDEI CTNAG 2 cut(s) 312, 422
BstKTI GATC 4 cut(s) 37, 271, 484, 722
BstMAI GTCTC 2 cut(s) 308, 481
BstMBI GATC 4 cut(s) 34, 268, 481, 719
BstMWI GCNNNNNNNGC 2 cut(s) 119, 289
BstNI CCWGG 2 cut(s) 492, 734
BstNSI RCATGY 1 cut(s) 409
BstSCI CCNGG 3 cut(s) 490, 704, 732
BstSFI CTRYAG 1 cut(s) 471
BstV1I GCAGC 1 cut(s) 457
BstX2I RGATCY 2 cut(s) 481, 719
BstYI RGATCY 2 cut(s) 481, 719
BsuI GTATCC 1 cut(s) 529
BsuRI GGCC 2 cut(s) 605, 691
BtsIMutI CAGTG 2 cut(s) 305, 518
Cac8I GCNNGC 2 cut(s) 51, 689
Cfr13I GGNCC 4 cut(s) 80, 262, 436, 460
Csp6I GTAC 3 cut(s) 184, 326, 371
CviAII CATG 4 cut(s) 212, 219, 406, 712
CviQI GTAC 3 cut(s) 184, 326, 371
DdeI CTNAG 2 cut(s) 312, 422
DpnI GATC 4 cut(s) 36, 270, 483, 721
DpnII GATC 4 cut(s) 34, 268, 481, 719
DraIII CACNNNGTG 1 cut(s) 43
EaeI YGGCCR 1 cut(s) 603
Ecl136II GAGCTC 1 cut(s) 379
Eco130I CCWWGG 1 cut(s) 606
Eco24I GRGCYC 1 cut(s) 381
Eco31I GGTCTC 1 cut(s) 481
Eco47I GGWCC 4 cut(s) 80, 262, 436, 460
Eco53kI GAGCTC 1 cut(s) 379
Eco57I CTGAAG 2 cut(s) 140, 618
Eco88I CYCGRG 1 cut(s) 380
EcoICRI GAGCTC 1 cut(s) 379
EcoRII CCWGG 2 cut(s) 490, 732
EcoT14I CCWWGG 1 cut(s) 606
EcoT38I GRGCYC 1 cut(s) 381
ErhI CCWWGG 1 cut(s) 606
FaeI CATG 4 cut(s) 215, 222, 409, 715
FaiI YATR 9 cut(s) 213, 220, 347, 407, 411, 419, 509, 593, 713
FaqI GGGAC 2 cut(s) 128, 248
FatI CATG 4 cut(s) 211, 218, 405, 711
FbaI TGATCA 2 cut(s) 34, 268
Fnu4HI GCNGC 1 cut(s) 471
FriOI GRGCYC 1 cut(s) 381
Fsp4HI GCNGC 1 cut(s) 471
FspBI CTAG 1 cut(s) 62
GluI GCNGC 1 cut(s) 471
HaeIII GGCC 2 cut(s) 605, 691
HapII CCGG 1 cut(s) 705
Hin1II CATG 4 cut(s) 215, 222, 409, 715
HinfI GANTC 1 cut(s) 511
HpaII CCGG 1 cut(s) 705
HphI GGTGA 1 cut(s) 91
Hpy166II GTNNAC 2 cut(s) 184, 254
Hpy188I TCNGA 3 cut(s) 130, 486, 729
Hpy188III TCNNGA 2 cut(s) 566, 667
Hpy8I GTNNAC 2 cut(s) 184, 254
Hpy99I CGWCG 2 cut(s) 92, 252
HpyAV CCTTC 1 cut(s) 158
HpyCH4III ACNGT 4 cut(s) 298, 333, 522, 682
HpyCH4IV ACGT 1 cut(s) 42
HpyCH4V TGCA 4 cut(s) 209, 349, 405, 473
HpyF10VI GCNNNNNNNGC 2 cut(s) 119, 289
HpyF3I CTNAG 2 cut(s) 312, 422
HpySE526I ACGT 1 cut(s) 42
Hsp92II CATG 4 cut(s) 215, 222, 409, 715
Ksp22I TGATCA 2 cut(s) 34, 268
Kzo9I GATC 4 cut(s) 34, 268, 481, 719
LmnI GCTCC 1 cut(s) 74
Lsp1109I GCAGC 1 cut(s) 457
LweI GCATC 1 cut(s) 229
MaeI CTAG 1 cut(s) 62
MaeII ACGT 1 cut(s) 42
MaeIII GTNAC 1 cut(s) 97
MalI GATC 4 cut(s) 36, 270, 483, 721
MbiI CCGCTC 1 cut(s) 74
MboI GATC 4 cut(s) 34, 268, 481, 719
MboII GAAGA 3 cut(s) 146, 542, 653
MflI RGATCY 2 cut(s) 481, 719
MhlI GDGCHC 1 cut(s) 381
MlsI TGGCCA 1 cut(s) 605
MluCI AATT 3 cut(s) 132, 175, 561
MluNI TGGCCA 1 cut(s) 605
MmeI TCCRAC 2 cut(s) 513, 607
Mox20I TGGCCA 1 cut(s) 605
MscI TGGCCA 1 cut(s) 605
MseI TTAA 2 cut(s) 278, 396
MslI CAYNNNNRTG 1 cut(s) 410
Msp20I TGGCCA 1 cut(s) 605
MspI CCGG 1 cut(s) 705
MspR9I CCNGG 3 cut(s) 492, 706, 734
MvaI CCWGG 2 cut(s) 492, 734
MwoI GCNNNNNNNGC 2 cut(s) 119, 289
NciI CCSGG 1 cut(s) 706
NdeII GATC 4 cut(s) 34, 268, 481, 719
NlaIII CATG 4 cut(s) 215, 222, 409, 715
NlaIV GGNNCC 2 cut(s) 70, 264
NmuCI GTSAC 1 cut(s) 97
NspI RCATGY 1 cut(s) 409
PaeR7I CTCGAG 1 cut(s) 380
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PfeI GAWTC 1 cut(s) 511
PkrI GCNGC 1 cut(s) 472
Ppu21I YACGTR 1 cut(s) 43
PshBI ATTAAT 1 cut(s) 396
Psp124BI GAGCTC 1 cut(s) 381
Psp6I CCWGG 2 cut(s) 490, 732
PspGI CCWGG 2 cut(s) 490, 732
PspN4I GGNNCC 2 cut(s) 70, 264
PspPI GGNCC 4 cut(s) 80, 262, 436, 460
PspXI VCTCGAGB 1 cut(s) 380
PstI CTGCAG 1 cut(s) 475
PsuI RGATCY 2 cut(s) 481, 719
RsaI GTAC 3 cut(s) 185, 327, 372
RsaNI GTAC 3 cut(s) 184, 326, 371
RseI CAYNNNNRTG 1 cut(s) 410
SacI GAGCTC 1 cut(s) 381
SaqAI TTAA 2 cut(s) 278, 396
SatI GCNGC 1 cut(s) 471
Sau3AI GATC 4 cut(s) 34, 268, 481, 719
Sau96I GGNCC 4 cut(s) 80, 262, 436, 460
ScrFI CCNGG 3 cut(s) 492, 706, 734
SduI GDGCHC 1 cut(s) 381
SfaNI GCATC 1 cut(s) 229
SfcI CTRYAG 1 cut(s) 471
Sfr274I CTCGAG 1 cut(s) 380
SinI GGWCC 4 cut(s) 80, 262, 436, 460
SlaI CTCGAG 1 cut(s) 380
SmiMI CAYNNNNRTG 1 cut(s) 410
SmlI CTYRAG 2 cut(s) 380, 541
SmoI CTYRAG 2 cut(s) 380, 541
Sse9I AATT 3 cut(s) 132, 175, 561
SsiI CCGC 1 cut(s) 72
SspMI CTAG 1 cut(s) 62
SstI GAGCTC 1 cut(s) 381
StyD4I CCNGG 3 cut(s) 490, 704, 732
StyI CCWWGG 1 cut(s) 606
TaaI ACNGT 4 cut(s) 298, 333, 522, 682
TaiI ACGT 1 cut(s) 45
TaqI TCGA 4 cut(s) 189, 199, 381, 656
TaqII GACCGA 1 cut(s) 68
TasI AATT 3 cut(s) 132, 175, 561
TfiI GAWTC 1 cut(s) 511
Tru1I TTAA 2 cut(s) 278, 396
Tru9I TTAA 2 cut(s) 278, 396
TscAI CASTG 2 cut(s) 312, 525
TseFI GTSAC 1 cut(s) 97
TseI GCWGC 1 cut(s) 470
Tsp45I GTSAC 1 cut(s) 97
TspDTI ATGAA 1 cut(s) 590
TspRI CASTG 2 cut(s) 312, 525
VpaK11BI GGWCC 4 cut(s) 80, 262, 436, 460
VspI ATTAAT 1 cut(s) 396
XapI RAATTY 1 cut(s) 175
XceI RCATGY 1 cut(s) 409
XhoI CTCGAG 1 cut(s) 380
XspI CTAG 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.