pycom07g26670

Protein phosphatase 2C 57-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0002125
Physical Location & Seq
Forward (+)
26639295 .. 26642815
3521 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1353 bp
ATGAATGTAAAGGGGTCCCACGTTCCTACCCAATCTGTTAATCCAGTTAGAGGTGCAGCTTCATTCTCGCTTTATCCTTCACAACAACAATTCCACCTCCACCACGTTCCTCCTAGTTCCGAACTAACTCGTCCCAACTCACCGACTCAACTCAGTGCCAGTAGTGCTACTACTGCTGGTGATAAAATGACTTTGATAAGCCCACAGCTGCAGAGGTTCGTCCTAACTAAATACCACGGCGGCTCATTCAAGACTGCCGCCGCAAAAAGCAGCTTCAACATAAAAGCAAGAAGCACAAGCTGCTCGGCGATTGCCATCGATGCGCCGGGTTCTTCCCTGACCGACGTGGCTGGAATCCGATGGGGTTCGACGAGTCTGCAGGGTGCCCGGGAGGAGATGGAAGATGCCGTCGTTGTCCGGTCTGAAGGCCTTGAAGAGTTCTCATTTGCCGCCGTTTTTGACGGCCATGCAGGGTTCAACTCGGTCAAGTTCCTCAGAGATGAGCTCTACAAGGAGTGTTGTGCGGCTTTGCAGGGCGGGCTGCTACTACGTGGAAACGACTTCAAGGCCATTAGAGAGGCATTGCTGGAGACGTTTGAAAAAGTTGACTCTAAATTGTTAAATTGGCTCGAAAGCAATGGGGAGGAAGACGGATCTGGTTCGACAGCTACCCTTATGTTCGTTGGAAACGATACGCTGGTTATTTCACACGTTGGTGATTCTTGTGTGGTTCAATCTTGTTCTGGAAAGGCAGAGGTTCTTACGCATCCTCATAGACCGTACGGGAGCAACAAGGCTTCTCTTCGAGAAATCAAAAGAATCAGAGAAGAAGGTGGATGGATCAGCAATGGAAGAATTTGTGGCGACATTGCTGTTTCTCGTGCGTTTGGTGACATGCGGTTCAAGACAAAGAAAAACGAGATGCTGAAAAAAGGAGTTGAAGAAAGGAGATGGACAGAAAAATTTGCTTCTCGAGTACAATTCAACGGTGACCTTGTTACTGCATCTCCAGACGTTTTTCAAGTAACTTTCGGGACAGATTCAGAATTTGTTCTGTTAGCATCAGATGGCTTATGGGACTACATAAACAGCTCAGATGCGGTGACTTTTGTAAGGAATCAGCTTCGAAAACACGGAGATGTTCAGCTAGCTTGCGAAGCGCTTGCTGAAGCTGCTCTGGATCAACGCTCGCAAGATAACATCAGCATTGTTATTGCAGATTTAGGGCGGACAGACTGGCAAGGTTTGCCATTTCAACAACAAAATGTTGTATATGAATTCGGCCAAGCTTTTGCTACGGTTGGGATTGTGTCGCTCGGAATTTGGATCTCGACATCTTTGCTTTCAACATGA

Protein Analysis

451

Amino Acids

49.01

Weight (kDa)

5.98

Isoelectric Point (pI)

44.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 122 - 401 3.6e-51 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 383
AciI CCGC 9 cut(s) 240, 258, 261, 450, 524, 537, 898, 1100, 1228
AclWI GGATC 4 cut(s) 661, 848, 1188, 1334
AcoI YGGCCR 2 cut(s) 463, 1282
AcsI RAATTY 5 cut(s) 855, 962, 1046, 1277, 1320
AcuI CTGAAG 2 cut(s) 444, 1188
AfaI GTAC 2 cut(s) 782, 978
AfeI AGCGCT 1 cut(s) 1161
AfiI CCNNNNNNNGG 1 cut(s) 50
AflIII ACRYGT 1 cut(s) 709
AjiI CACGTC 1 cut(s) 346
AleI CACNNNNGTG 1 cut(s) 714
Alw21I GWGCWC 1 cut(s) 507
Alw26I GTCTC 1 cut(s) 584
AlwI GGATC 4 cut(s) 661, 848, 1188, 1334
Ama87I CYCGRG 2 cut(s) 387, 972
Aor51HI AGCGCT 1 cut(s) 1161
AoxI GGCC 4 cut(s) 427, 463, 567, 1282
ApeKI GCWGC 6 cut(s) 56, 208, 270, 300, 541, 1172
ApoI RAATTY 5 cut(s) 855, 962, 1046, 1277, 1320
Asp700I GAANNNNTTC 1 cut(s) 1050
AspLEI GCGC 2 cut(s) 325, 1162
AspS9I GGNCC 1 cut(s) 15
AsuC2I CCSGG 3 cut(s) 327, 388, 389
AsuHPI GGTGA 6 cut(s) 132, 191, 728, 902, 1001, 1114
AsuII TTCGAA 1 cut(s) 1126
AsuNHI GCTAGC 1 cut(s) 1147
AvaI CYCGRG 2 cut(s) 387, 972
AvaII GGWCC 1 cut(s) 15
BaeGI GKGCMC 1 cut(s) 388
BaeI ACNNNNGTAYC 2 cut(s) 684, 717
BanI GGYRCC 1 cut(s) 383
BanII GRGCYC 1 cut(s) 507
BauI CACGAG 1 cut(s) 879
BbsI GAAGAC 1 cut(s) 654
Bbv12I GWGCWC 1 cut(s) 507
BbvI GCAGC 6 cut(s) 68, 195, 282, 287, 528, 1159
BccI CCATC 6 cut(s) 323, 354, 391, 831, 945, 1061
BceAI ACGGC 4 cut(s) 253, 392, 437, 478
BcgI CGANNNNNNTGC 5 cut(s) 358, 392, 1145, 1179, 1321
BcnI CCSGG 3 cut(s) 327, 388, 389
BcoDI GTCTC 1 cut(s) 584
BfaI CTAG 2 cut(s) 114, 1148
BfmI CTRYAG 2 cut(s) 209, 377
BfoI RGCGCY 1 cut(s) 1163
Bme1390I CCNGG 3 cut(s) 327, 388, 389
Bme18I GGWCC 1 cut(s) 15
BmeT110I CYCGRG 2 cut(s) 387, 972
BmgBI CACGTC 1 cut(s) 346
BmgT120I GGNCC 1 cut(s) 15
BmiI GGNNCC 3 cut(s) 16, 17, 385
BmrFI CCNGG 3 cut(s) 327, 388, 389
BmsI GCATC 7 cut(s) 310, 394, 775, 912, 1013, 1070, 1087
BmtI GCTAGC 1 cut(s) 1151
BpiI GAAGAC 1 cut(s) 654
BplI GAGNNNNNCTC 2 cut(s) 489, 521
BpmI CTGGAG 2 cut(s) 608, 993
Bpu14I TTCGAA 1 cut(s) 1126
BpuMI CCSGG 3 cut(s) 327, 388, 389
Bsa29I ATCGAT 1 cut(s) 318
BsaAI YACGTR 1 cut(s) 551
BsaBI GATNNNNATC 1 cut(s) 314
BsaJI CCNNGG 2 cut(s) 235, 387
BsaWI WCCGGW 1 cut(s) 417
BsaXI ACNNNNNCTCC 2 cut(s) 991, 1021
Bsc4I CCNNNNNNNGG 1 cut(s) 50
Bse1I ACTGG 3 cut(s) 44, 159, 1241
Bse3DI GCAATG 4 cut(s) 581, 643, 853, 867
Bse8I GATNNNNATC 1 cut(s) 314
BseCI ATCGAT 1 cut(s) 318
BseDI CCNNGG 2 cut(s) 235, 387
BseGI GGATG 2 cut(s) 766, 842
BseJI GATNNNNATC 1 cut(s) 314
BseLI CCNNNNNNNGG 1 cut(s) 50
BseMI GCAATG 4 cut(s) 581, 643, 853, 867
BseMII CTCAG 3 cut(s) 166, 508, 1107
BseNI ACTGG 3 cut(s) 44, 159, 1241
BseRI GAGGAG 1 cut(s) 407
BseSI GKGCMC 1 cut(s) 388
BseXI GCAGC 6 cut(s) 68, 195, 282, 287, 528, 1159
BsgI GTGCAG 1 cut(s) 75
BshFI GGCC 4 cut(s) 429, 465, 569, 1284
BshNI GGYRCC 1 cut(s) 383
BshVI ATCGAT 1 cut(s) 318
BsiHKAI GWGCWC 1 cut(s) 507
BsiHKCI CYCGRG 2 cut(s) 387, 972
BsiSI CCGG 3 cut(s) 326, 388, 418
BsiWI CGTACG 1 cut(s) 780
BslFI GGGAC 3 cut(s) 117, 1048, 1091
BslI CCNNNNNNNGG 1 cut(s) 50
BsmAI GTCTC 1 cut(s) 584
BsmBI CGTCTC 1 cut(s) 584
BsmFI GGGAC 3 cut(s) 117, 1048, 1091
BsnI GGCC 4 cut(s) 429, 465, 569, 1284
BsoBI CYCGRG 2 cut(s) 387, 972
Bsp119I TTCGAA 1 cut(s) 1126
Bsp1286I GDGCHC 2 cut(s) 388, 507
Bsp143I GATC 4 cut(s) 653, 840, 1180, 1326
BspACI CCGC 9 cut(s) 240, 258, 261, 450, 524, 537, 898, 1100, 1228
BspANI GGCC 4 cut(s) 429, 465, 569, 1284
BspCNI CTCAG 3 cut(s) 165, 507, 1106
BspDI ATCGAT 1 cut(s) 318
BspLI GGNNCC 3 cut(s) 16, 17, 385
BspMAI CTGCAG 2 cut(s) 213, 381
BspOI GCTAGC 1 cut(s) 1151
BspPI GGATC 4 cut(s) 661, 848, 1188, 1334
BspT104I TTCGAA 1 cut(s) 1126
BspT107I GGYRCC 1 cut(s) 383
BsrDI GCAATG 4 cut(s) 581, 643, 853, 867
BsrI ACTGG 3 cut(s) 44, 159, 1241
BssECI CCNNGG 2 cut(s) 235, 387
BssMI GATC 4 cut(s) 653, 840, 1180, 1326
BssSI CACGAG 1 cut(s) 879
Bst2BI CACGAG 1 cut(s) 879
Bst4CI ACNGT 3 cut(s) 780, 989, 1300
Bst6I CTCTTC 2 cut(s) 429, 807
BstAPI GCANNNNNTGC 2 cut(s) 300, 1246
BstBAI YACGTR 1 cut(s) 551
BstBI TTCGAA 1 cut(s) 1126
BstC8I GCNNGC 5 cut(s) 539, 1149, 1153, 1164, 1190
BstDEI CTNAG 3 cut(s) 152, 494, 1093
BstDSI CCRYGG 1 cut(s) 235
BstEII GGTNACC 1 cut(s) 989
BstF5I GGATG 2 cut(s) 766, 842
BstH2I RGCGCY 1 cut(s) 1163
BstHHI GCGC 2 cut(s) 325, 1162
BstKTI GATC 4 cut(s) 656, 843, 1183, 1329
BstMAI GTCTC 1 cut(s) 584
BstMBI GATC 4 cut(s) 653, 840, 1180, 1326
BstMWI GCNNNNNNNGC 8 cut(s) 164, 173, 300, 320, 538, 1157, 1172, 1246
BstNSI RCATGY 1 cut(s) 898
BstPI GGTNACC 1 cut(s) 989
BstSCI CCNGG 3 cut(s) 325, 386, 387
BstSFI CTRYAG 2 cut(s) 209, 377
BstSLI GKGCMC 1 cut(s) 388
BstV1I GCAGC 6 cut(s) 68, 195, 282, 287, 528, 1159
BstV2I GAAGAC 1 cut(s) 654
BstX2I RGATCY 2 cut(s) 653, 1326
BstYI RGATCY 2 cut(s) 653, 1326
Bsu15I ATCGAT 1 cut(s) 318
BsuRI GGCC 4 cut(s) 429, 465, 569, 1284
BsuTUI ATCGAT 1 cut(s) 318
BtgI CCRYGG 1 cut(s) 235
BtrI CACGTC 1 cut(s) 346
BtsCI GGATG 2 cut(s) 766, 842
BtsIMutI CAGTG 1 cut(s) 160
Cac8I GCNNGC 5 cut(s) 539, 1149, 1153, 1164, 1190
CfoI GCGC 2 cut(s) 325, 1162
Cfr13I GGNCC 1 cut(s) 15
Cfr9I CCCGGG 1 cut(s) 387
ClaI ATCGAT 1 cut(s) 318
Csp6I GTAC 2 cut(s) 781, 977
CviAII CATG 3 cut(s) 467, 895, 1350
CviQI GTAC 2 cut(s) 781, 977
DdeI CTNAG 3 cut(s) 152, 494, 1093
DpnI GATC 4 cut(s) 655, 842, 1182, 1328
DpnII GATC 4 cut(s) 653, 840, 1180, 1326
EaeI YGGCCR 2 cut(s) 463, 1282
Eam1104I CTCTTC 2 cut(s) 429, 807
EarI CTCTTC 2 cut(s) 429, 807
EciI GGCGGA 1 cut(s) 1243
Ecl136II GAGCTC 1 cut(s) 505
Eco147I AGGCCT 1 cut(s) 429
Eco24I GRGCYC 1 cut(s) 507
Eco47I GGWCC 1 cut(s) 15
Eco47III AGCGCT 1 cut(s) 1161
Eco53kI GAGCTC 1 cut(s) 505
Eco57I CTGAAG 2 cut(s) 444, 1188
Eco88I CYCGRG 2 cut(s) 387, 972
Eco91I GGTNACC 1 cut(s) 989
EcoICRI GAGCTC 1 cut(s) 505
EcoO109I RGGNCCY 1 cut(s) 15
EcoO65I GGTNACC 1 cut(s) 989
EcoRI GAATTC 1 cut(s) 1277
EcoT38I GRGCYC 1 cut(s) 507
Esp3I CGTCTC 1 cut(s) 584
FaeI CATG 3 cut(s) 470, 898, 1353
FaqI GGGAC 3 cut(s) 117, 1048, 1091
FatI CATG 3 cut(s) 466, 894, 1349
FauI CCCGC 1 cut(s) 530
FokI GGATG 2 cut(s) 753, 849
FriOI GRGCYC 1 cut(s) 507
FspBI CTAG 2 cut(s) 114, 1148
GlaI GCGC 2 cut(s) 324, 1161
GsuI CTGGAG 2 cut(s) 608, 993
HaeII RGCGCY 1 cut(s) 1163
HaeIII GGCC 4 cut(s) 429, 465, 569, 1284
HapII CCGG 3 cut(s) 326, 388, 418
HhaI GCGC 2 cut(s) 325, 1162
Hin1II CATG 3 cut(s) 470, 898, 1353
Hin6I GCGC 2 cut(s) 323, 1160
HinP1I GCGC 2 cut(s) 323, 1160
HincII GTYRAC 1 cut(s) 607
HindII GTYRAC 1 cut(s) 607
HindIII AAGCTT 1 cut(s) 1287
HinfI GANTC 8 cut(s) 145, 354, 373, 608, 719, 819, 1040, 1117
HpaII CCGG 3 cut(s) 326, 388, 418
HphI GGTGA 6 cut(s) 132, 191, 728, 902, 1001, 1114
Hpy166II GTNNAC 1 cut(s) 607
Hpy188I TCNGA 9 cut(s) 121, 359, 424, 497, 824, 1045, 1066, 1096, 1319
Hpy188III TCNNGA 9 cut(s) 250, 744, 806, 904, 972, 1010, 1033, 1178, 1330
Hpy8I GTNNAC 1 cut(s) 607
Hpy99I CGWCG 3 cut(s) 347, 373, 413
HpyAV CCTTC 3 cut(s) 87, 419, 824
HpyCH4III ACNGT 3 cut(s) 780, 989, 1300
HpyCH4IV ACGT 7 cut(s) 21, 105, 345, 550, 593, 711, 1014
HpyCH4V TGCA 7 cut(s) 56, 211, 379, 470, 532, 1004, 1217
HpyF10VI GCNNNNNNNGC 8 cut(s) 164, 173, 300, 320, 538, 1157, 1172, 1246
HpyF3I CTNAG 3 cut(s) 152, 494, 1093
HpySE526I ACGT 7 cut(s) 21, 105, 345, 550, 593, 711, 1014
Hsp92II CATG 3 cut(s) 470, 898, 1353
HspAI GCGC 2 cut(s) 323, 1160
KflI GGGWCCC 1 cut(s) 15
Kzo9I GATC 4 cut(s) 653, 840, 1180, 1326
LmnI GCTCC 1 cut(s) 786
Lsp1109I GCAGC 6 cut(s) 68, 195, 282, 287, 528, 1159
LweI GCATC 7 cut(s) 310, 394, 775, 912, 1013, 1070, 1087
MaeI CTAG 2 cut(s) 114, 1148
MaeII ACGT 7 cut(s) 21, 105, 345, 550, 593, 711, 1014
MaeIII GTNAC 5 cut(s) 890, 989, 997, 1024, 1102
MalI GATC 4 cut(s) 655, 842, 1182, 1328
MboI GATC 4 cut(s) 653, 840, 1180, 1326
MboII GAAGA 8 cut(s) 324, 413, 446, 659, 794, 839, 864, 953
MflI RGATCY 2 cut(s) 653, 1326
MhlI GDGCHC 2 cut(s) 388, 507
MluCI AATT 9 cut(s) 89, 614, 622, 855, 962, 980, 1046, 1277, 1320
MlyI GAGTC 3 cut(s) 139, 382, 602
MmeI TCCRAC 1 cut(s) 664
MroXI GAANNNNTTC 1 cut(s) 1050
MseI TTAA 2 cut(s) 39, 620
MslI CAYNNNNRTG 2 cut(s) 714, 1137
MspA1I CMGCKG 1 cut(s) 208
MspI CCGG 3 cut(s) 326, 388, 418
MspR9I CCNGG 3 cut(s) 327, 388, 389
MwoI GCNNNNNNNGC 8 cut(s) 164, 173, 300, 320, 538, 1157, 1172, 1246
NciI CCSGG 3 cut(s) 327, 388, 389
NdeII GATC 4 cut(s) 653, 840, 1180, 1326
NheI GCTAGC 1 cut(s) 1147
NlaIII CATG 3 cut(s) 470, 898, 1353
NlaIV GGNNCC 3 cut(s) 16, 17, 385
NmeAIII GCCGAG 1 cut(s) 284
NmuCI GTSAC 3 cut(s) 890, 989, 1102
NspI RCATGY 1 cut(s) 898
NspV TTCGAA 1 cut(s) 1126
OliI CACNNNNGTG 1 cut(s) 714
PaeR7I CTCGAG 1 cut(s) 972
PceI AGGCCT 1 cut(s) 429
PcsI WCGNNNNNNNCGW 1 cut(s) 687
PdmI GAANNNNTTC 1 cut(s) 1050
PfeI GAWTC 5 cut(s) 354, 719, 819, 1040, 1117
Pfl23II CGTACG 1 cut(s) 780
PleI GAGTC 3 cut(s) 139, 381, 602
PpsI GAGTC 3 cut(s) 139, 381, 602
Ppu21I YACGTR 1 cut(s) 551
PpuMI RGGWCCY 1 cut(s) 15
Psp124BI GAGCTC 1 cut(s) 507
Psp5II RGGWCCY 1 cut(s) 15
PspEI GGTNACC 1 cut(s) 989
PspLI CGTACG 1 cut(s) 780
PspN4I GGNNCC 3 cut(s) 16, 17, 385
PspPI GGNCC 1 cut(s) 15
PspPPI RGGWCCY 1 cut(s) 15
PstI CTGCAG 2 cut(s) 213, 381
PsuI RGATCY 2 cut(s) 653, 1326
PvuII CAGCTG 1 cut(s) 208
RsaI GTAC 2 cut(s) 782, 978
RsaNI GTAC 2 cut(s) 781, 977
RseI CAYNNNNRTG 2 cut(s) 714, 1137
SacI GAGCTC 1 cut(s) 507
SaqAI TTAA 2 cut(s) 39, 620
Sau3AI GATC 4 cut(s) 653, 840, 1180, 1326
Sau96I GGNCC 1 cut(s) 15
SchI GAGTC 3 cut(s) 139, 382, 602
ScrFI CCNGG 3 cut(s) 327, 388, 389
SduI GDGCHC 2 cut(s) 388, 507
SfaNI GCATC 7 cut(s) 310, 394, 775, 912, 1013, 1070, 1087
SfcI CTRYAG 2 cut(s) 209, 377
Sfr274I CTCGAG 1 cut(s) 972
SfuI TTCGAA 1 cut(s) 1126
SinI GGWCC 1 cut(s) 15
SlaI CTCGAG 1 cut(s) 972
SmaI CCCGGG 1 cut(s) 389
SmiMI CAYNNNNRTG 2 cut(s) 714, 1137
SmlI CTYRAG 1 cut(s) 972
SmoI CTYRAG 1 cut(s) 972
Sse9I AATT 9 cut(s) 89, 614, 622, 855, 962, 980, 1046, 1277, 1320
SseBI AGGCCT 1 cut(s) 429
SsiI CCGC 9 cut(s) 240, 258, 261, 450, 524, 537, 898, 1100, 1228
SspMI CTAG 2 cut(s) 114, 1148
SstI GAGCTC 1 cut(s) 507
StuI AGGCCT 1 cut(s) 429
StyD4I CCNGG 3 cut(s) 325, 386, 387
TaaI ACNGT 3 cut(s) 780, 989, 1300
TaiI ACGT 7 cut(s) 24, 108, 348, 553, 596, 714, 1017
TaqI TCGA 8 cut(s) 318, 368, 630, 662, 805, 973, 1126, 1331
TaqII GACCGA 2 cut(s) 356, 472
TasI AATT 9 cut(s) 89, 614, 622, 855, 962, 980, 1046, 1277, 1320
TatI WGTACW 1 cut(s) 976
TauI GCSGC 5 cut(s) 243, 260, 263, 452, 527
TfiI GAWTC 5 cut(s) 354, 719, 819, 1040, 1117
Tru1I TTAA 2 cut(s) 39, 620
Tru9I TTAA 2 cut(s) 39, 620
TscAI CASTG 1 cut(s) 160
TseFI GTSAC 3 cut(s) 890, 989, 1102
TseI GCWGC 6 cut(s) 56, 208, 270, 300, 541, 1172
Tsp45I GTSAC 3 cut(s) 890, 989, 1102
TspDTI ATGAA 3 cut(s) 17, 51, 1290
TspGWI ACGGA 2 cut(s) 666, 1149
TspMI CCCGGG 1 cut(s) 387
TspRI CASTG 1 cut(s) 160
VpaK11BI GGWCC 1 cut(s) 15
XapI RAATTY 5 cut(s) 855, 962, 1046, 1277, 1320
XceI RCATGY 1 cut(s) 898
XhoI CTCGAG 1 cut(s) 972
XmaI CCCGGG 1 cut(s) 387
XmnI GAANNNNTTC 1 cut(s) 1050
XspI CTAG 2 cut(s) 114, 1148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.