pycom09g04770

Transmembrane protein 256 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0002952
Physical Location & Seq
Reverse (-)
3528441 .. 3529611
1171 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 432 bp
ATGTATAAATCTCATCATCCACGTAGTGCATGGAATTTTGAAACATATTTACTAAGTGAGAGAGGCACTGAAACAGAAGCAAGAGAAGCAACAACAATGGATCTTCAGCTGTGGCATAAAGCAGCGGCGCTCTCTGGAATTGCAGCTGTTGGGTTGGGTACATACGGCGCCCTTGGCTTCAAACCCAAAGACCCAACTTACAAAGAGGTTTGGCTGACGGCGTCTCTGTACCATTTGGTTCACACAGCAGCTCTGCTTGCTGCCCCCATCGCGAAACACCCCACCCTTTTTGGAGGCCTTTTGGCTGCTGGAATACTCGCATTATCCGGGACGTATTATTTTGTGGCATTACTTGAGGATAGAAAGTACTTGAAATTGGCTCTTTTCGGTGGCTTTGTTTTCATCGCTGCTTGGGGTAGTTTGCTGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF423

Protein Analysis

144

Amino Acids

15.65

Weight (kDa)

8.83

Isoelectric Point (pI)

23.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF423 PF04241 52 - 131 9e-15 Protein of unknown function (DUF423)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 167
AccII CGCG 1 cut(s) 272
AciI CCGC 1 cut(s) 125
AclWI GGATC 1 cut(s) 108
AcsI RAATTY 1 cut(s) 34
AcuI CTGAAG 1 cut(s) 89
AcyI GRCGYC 2 cut(s) 168, 221
AdeI CACNNNGTG 1 cut(s) 26
AfaI GTAC 3 cut(s) 160, 230, 368
AgsI TTSAA 3 cut(s) 41, 181, 373
AluBI AGCT 3 cut(s) 109, 146, 251
AluI AGCT 3 cut(s) 109, 146, 251
Alw26I GTCTC 1 cut(s) 228
AlwI GGATC 1 cut(s) 108
AoxI GGCC 1 cut(s) 295
ApeKI GCWGC 6 cut(s) 122, 143, 248, 260, 305, 407
ApoI RAATTY 1 cut(s) 34
AspLEI GCGC 2 cut(s) 130, 170
AsuC2I CCSGG 1 cut(s) 328
BanI GGYRCC 1 cut(s) 167
BbvI GCAGC 6 cut(s) 134, 155, 247, 260, 292, 394
BccI CCATC 1 cut(s) 275
BceAI ACGGC 2 cut(s) 181, 234
BcnI CCSGG 1 cut(s) 328
BcoDI GTCTC 1 cut(s) 228
BfoI RGCGCY 2 cut(s) 131, 171
BisI GCNGC 7 cut(s) 123, 126, 144, 249, 261, 306, 408
BlsI GCNGC 7 cut(s) 124, 127, 145, 250, 262, 307, 409
BmcAI AGTACT 1 cut(s) 368
Bme1390I CCNGG 1 cut(s) 328
BmiI GGNNCC 1 cut(s) 169
BmrFI CCNGG 1 cut(s) 328
BpuEI CTTGAG 1 cut(s) 374
BpuMI CCSGG 1 cut(s) 328
BsaAI YACGTR 1 cut(s) 23
BsaHI GRCGYC 2 cut(s) 168, 221
BsaJI CCNNGG 1 cut(s) 172
BseDI CCNNGG 1 cut(s) 172
BseGI GGATG 1 cut(s) 16
BseXI GCAGC 6 cut(s) 134, 155, 247, 260, 292, 394
Bsh1236I CGCG 1 cut(s) 272
BshFI GGCC 1 cut(s) 297
BshNI GGYRCC 1 cut(s) 167
BsiSI CCGG 1 cut(s) 327
BslFI GGGAC 1 cut(s) 343
BsmAI GTCTC 1 cut(s) 228
BsmBI CGTCTC 1 cut(s) 228
BsmFI GGGAC 1 cut(s) 343
BsnI GGCC 1 cut(s) 297
Bsp143I GATC 1 cut(s) 100
Bsp68I TCGCGA 1 cut(s) 272
BspACI CCGC 1 cut(s) 125
BspANI GGCC 1 cut(s) 297
BspFNI CGCG 1 cut(s) 272
BspLI GGNNCC 1 cut(s) 169
BspPI GGATC 1 cut(s) 108
BspT107I GGYRCC 1 cut(s) 167
BssECI CCNNGG 1 cut(s) 172
BssMI GATC 1 cut(s) 100
BssNI GRCGYC 2 cut(s) 168, 221
BssT1I CCWWGG 1 cut(s) 172
BstACI GRCGYC 2 cut(s) 168, 221
BstBAI YACGTR 1 cut(s) 23
BstC8I GCNNGC 1 cut(s) 258
BstDEI CTNAG 1 cut(s) 53
BstF5I GGATG 1 cut(s) 16
BstFNI CGCG 1 cut(s) 272
BstH2I RGCGCY 2 cut(s) 131, 171
BstHHI GCGC 2 cut(s) 130, 170
BstKTI GATC 1 cut(s) 103
BstMAI GTCTC 1 cut(s) 228
BstMBI GATC 1 cut(s) 100
BstMWI GCNNNNNNNGC 4 cut(s) 86, 174, 257, 269
BstSCI CCNGG 1 cut(s) 326
BstUI CGCG 1 cut(s) 272
BstV1I GCAGC 6 cut(s) 134, 155, 247, 260, 292, 394
BstX2I RGATCY 1 cut(s) 100
BstYI RGATCY 1 cut(s) 100
BsuRI GGCC 1 cut(s) 297
BtgZI GCGATG 2 cut(s) 253, 388
BtsCI GGATG 1 cut(s) 16
BtsIMutI CAGTG 1 cut(s) 66
BtuMI TCGCGA 1 cut(s) 272
Cac8I GCNNGC 1 cut(s) 258
CfoI GCGC 2 cut(s) 130, 170
CseI GACGC 1 cut(s) 210
Csp6I GTAC 3 cut(s) 159, 229, 367
CspCI CAANNNNNGTGG 2 cut(s) 271, 306
CviAII CATG 1 cut(s) 30
CviJI RGCY 9 cut(s) 109, 146, 177, 214, 251, 297, 305, 380, 393
CviKI_1 RGCY 9 cut(s) 109, 146, 177, 214, 251, 297, 305, 380, 393
CviQI GTAC 3 cut(s) 159, 229, 367
DdeI CTNAG 1 cut(s) 53
DinI GGCGCC 1 cut(s) 169
DpnI GATC 1 cut(s) 102
DpnII GATC 1 cut(s) 100
DraIII CACNNNGTG 1 cut(s) 26
Eco130I CCWWGG 1 cut(s) 172
Eco147I AGGCCT 1 cut(s) 297
Eco57I CTGAAG 1 cut(s) 89
EcoT14I CCWWGG 1 cut(s) 172
EgeI GGCGCC 1 cut(s) 169
EheI GGCGCC 1 cut(s) 169
ErhI CCWWGG 1 cut(s) 172
Esp3I CGTCTC 1 cut(s) 228
FaeI CATG 1 cut(s) 33
FaiI YATR 5 cut(s) 6, 31, 46, 117, 163
FaqI GGGAC 1 cut(s) 343
FatI CATG 1 cut(s) 29
Fnu4HI GCNGC 7 cut(s) 123, 126, 144, 249, 261, 306, 408
FokI GGATG 1 cut(s) 3
Fsp4HI GCNGC 7 cut(s) 123, 126, 144, 249, 261, 306, 408
GlaI GCGC 2 cut(s) 129, 169
GluI GCNGC 7 cut(s) 123, 126, 144, 249, 261, 306, 408
HaeII RGCGCY 2 cut(s) 131, 171
HaeIII GGCC 1 cut(s) 297
HapII CCGG 1 cut(s) 327
HgaI GACGC 1 cut(s) 210
HhaI GCGC 2 cut(s) 130, 170
Hin1I GRCGYC 2 cut(s) 168, 221
Hin1II CATG 1 cut(s) 33
Hin6I GCGC 2 cut(s) 128, 168
HinP1I GCGC 2 cut(s) 128, 168
HpaII CCGG 1 cut(s) 327
Hpy166II GTNNAC 1 cut(s) 241
Hpy188III TCNNGA 2 cut(s) 135, 271
Hpy8I GTNNAC 1 cut(s) 241
HpyCH4IV ACGT 2 cut(s) 22, 332
HpyCH4V TGCA 2 cut(s) 29, 143
HpyF10VI GCNNNNNNNGC 4 cut(s) 86, 174, 257, 269
HpyF3I CTNAG 1 cut(s) 53
HpySE526I ACGT 2 cut(s) 22, 332
Hsp92I GRCGYC 2 cut(s) 168, 221
Hsp92II CATG 1 cut(s) 33
HspAI GCGC 2 cut(s) 128, 168
KasI GGCGCC 1 cut(s) 167
Kzo9I GATC 1 cut(s) 100
LpnPI CCDG 3 cut(s) 120, 294, 340
Lsp1109I GCAGC 6 cut(s) 134, 155, 247, 260, 292, 394
MaeII ACGT 2 cut(s) 22, 332
MalI GATC 1 cut(s) 102
MboI GATC 1 cut(s) 100
MboII GAAGA 1 cut(s) 95
MflI RGATCY 1 cut(s) 100
MluCI AATT 3 cut(s) 34, 138, 374
Mly113I GGCGCC 1 cut(s) 168
MnlI CCTC 4 cut(s) 56, 199, 287, 349
MspA1I CMGCKG 3 cut(s) 109, 125, 146
MspI CCGG 1 cut(s) 327
MspR9I CCNGG 1 cut(s) 328
MvnI CGCG 1 cut(s) 272
MwoI GCNNNNNNNGC 4 cut(s) 86, 174, 257, 269
NarI GGCGCC 1 cut(s) 168
NciI CCSGG 1 cut(s) 328
NdeII GATC 1 cut(s) 100
NlaIII CATG 1 cut(s) 33
NlaIV GGNNCC 1 cut(s) 169
NruI TCGCGA 1 cut(s) 272
PceI AGGCCT 1 cut(s) 297
PflFI GACNNNGTC 1 cut(s) 220
PfoI TCCNGGA 1 cut(s) 326
PkrI GCNGC 7 cut(s) 124, 127, 145, 250, 262, 307, 409
PluTI GGCGCC 1 cut(s) 171
Ppu21I YACGTR 1 cut(s) 23
PspN4I GGNNCC 1 cut(s) 169
PsuI RGATCY 1 cut(s) 100
PsyI GACNNNGTC 1 cut(s) 220
PvuII CAGCTG 2 cut(s) 109, 146
RruI TCGCGA 1 cut(s) 272
RsaI GTAC 3 cut(s) 160, 230, 368
RsaNI GTAC 3 cut(s) 159, 229, 367
SatI GCNGC 7 cut(s) 123, 126, 144, 249, 261, 306, 408
Sau3AI GATC 1 cut(s) 100
ScaI AGTACT 1 cut(s) 368
ScrFI CCNGG 1 cut(s) 328
SetI ASST 6 cut(s) 25, 111, 148, 210, 253, 335
SfoI GGCGCC 1 cut(s) 169
SmlI CTYRAG 1 cut(s) 353
SmoI CTYRAG 1 cut(s) 353
Sse9I AATT 3 cut(s) 34, 138, 374
SseBI AGGCCT 1 cut(s) 297
SsiI CCGC 1 cut(s) 125
SspDI GGCGCC 1 cut(s) 167
StuI AGGCCT 1 cut(s) 297
StyD4I CCNGG 1 cut(s) 326
StyI CCWWGG 1 cut(s) 172
TaiI ACGT 2 cut(s) 25, 335
TasI AATT 3 cut(s) 34, 138, 374
TatI WGTACW 1 cut(s) 366
TauI GCSGC 1 cut(s) 128
TscAI CASTG 1 cut(s) 73
TseI GCWGC 6 cut(s) 122, 143, 248, 260, 305, 407
TspDTI ATGAA 1 cut(s) 391
TspRI CASTG 1 cut(s) 73
Tth111I GACNNNGTC 1 cut(s) 220
XapI RAATTY 1 cut(s) 34
XcmI CCANNNNNNNNNTGG 1 cut(s) 27
ZrmI AGTACT 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.