Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr9

Orthogroup ID

OG0002908

Physical Location & Seq

Genomic Coordinates Forward (+)

5012092 .. 5015758

Gene Structure 3667 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1014 bp

ATGCATGTGTGGACAGAAGAGAGAGAGCTAAGCGCAGAGAAAAATAATAGAATTGTGAGAGAGAGAGAGAGAGAGAGAGATATGGAGGTGACAAAGGCGGTGAGATATTCATCAGAAAAGGTTGAGGATGAAGAGGAAGTGATGCTTCCAGGGTTTAGATTTTATCCAACAGATGAGGAGCTTGTTGGGTTTTATCTAAAGAGGAAGGTGGAGAAGAGAGCCATTCGTATGGAACTCATCAAACAGATTGATATCTACAAATATGATCCTTGGATCGATCTCCCAAAAGCTAGCAGCGTGGGGGACAATGAGTGCTACTTCTTTTGCAGAAGAGGAAGAAAATATAGGAACAGTGTAAGACCTAACAGAGTCACAGGTTCTGGATTTTGGAAAGCCACTGGGATTGACAAGCCTATAAATTCAGTTAAAGATCCACTCGAGTGCATTGGCCTCAAGAAATCATTAGTCTACTACCGTGGGAGTGCTGGCAAAGGCACCAAAACTGATTGGATGATGAATGAGTTTCGCCTTCCTCCTAATGGCCATGGCAAAAATGCCAAGTTCCTAAAGGCCAAAGTCGACGTTACACAAGAAGAAGCTGAGGTCTGGACACTCTGCCGAATTTTCAAACGAATCCCATCTTATAAAAAGTACACACCAGACTTCAAAGAATCCACGACCAGACAAAACCCTACTGATTCAAGTTCTAAAACATGCAGCTTCGAATCTGGGATCTACTGCAGTGAGCTACACGTCGGTCTCGAAGATTCGGTCTTTCAACGCATTGAAAGATCAAAACCAATTCATGTGGATGAACAAGTTGACGAAACAAATCACTGGCTTGGAGGGGAGTTGGACCCTATCTATGACCCAACTCATTTTGCAACAACTTATTCAAGCTTTAGGAACCCGAGCGACGGCGATTTCTTTACTAATGGAAACTGGGATGAGCTGAGACCAGTGGTTCAGCTGGCTGTTGATCCATCGTCCCAAGTTTATGACTGTAGAATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0005975 GO:0005984 GO:0005991 GO:0005992 GO:0006082 GO:0006355 GO:0006520 GO:0006560 GO:0006561 GO:0006725 GO:0006790 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009311 GO:0009312 GO:0009403 GO:0009404 GO:0009628 GO:0009651 GO:0009700 GO:0009718 GO:0009812 GO:0009813 GO:0009889 GO:0009987 GO:0010120 GO:0010150 GO:0010468 GO:0010556 GO:0016051 GO:0016053 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019748 GO:0019752 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0034637 GO:0034641 GO:0042430 GO:0042435 GO:0042440 GO:0042538 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044262 GO:0044271 GO:0044272 GO:0044281 GO:0044283 GO:0044550 GO:0046148 GO:0046217 GO:0046283 GO:0046351 GO:0046394 GO:0046483 GO:0048366 GO:0048367 GO:0048519 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051239 GO:0051241 GO:0051252 GO:0052314 GO:0052315 GO:0052317 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090693 GO:0099402 GO:0140110 GO:1900055 GO:1900056 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1903506 GO:1905622 GO:2000024 GO:2000026 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NAM

Protein Analysis

338

Amino Acids

39.36

Weight (kDa)

6.64

Isoelectric Point (pI)

34.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NAM	PF02365	50 - 177	2.9e-41	No apical meristem (NAM) protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002908)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G43000 AT2G43000
fragaria_vesca	FvH4_3g11860 FvH4_6g38750
malus_domestica	MD05G1258300.v1.1 MD09G1147500.v1.1 MD09G1147600.v1.1 MD10G1238200.v1.1 MD17G1134400.v1.1
prunus_persica	Prupe.3G178000_v2.0.a1 Prupe.3G178000_v2.0.a1 Prupe.4G106100_v2.0.a1 Prupe.4G106100_v2.0.a1
pyrus_communis	pycom05g24310 pycom09g06780 pycom10g19920 pycom17g12860
rosa_chinensis	RchiOBHm_Chr2g0152271 RchiOBHm_Chr2g0152301 RchiOBHm_Chr5g0019291
rosa_laevigata	RLG00000020626 RLG00000032435
rosa_multiflora	Rmu_sc0004002.1_g000004 Rmu_sc0004608.1_g000002
rosa_roxburghii	Rroxscaffold_1G00058710 Rroxscaffold_1G00074210 Rroxscaffold_2G00095810
rosa_rugosa	Rorug02G0431900 Rorug05G0049400
rosa_samantha	Rh2AG493700 Rh2BG506000 Rh2CG479900 Rh2DG517100 Rh5AG140500 Rh5CG150800 Rh5DG140000
rosa_wichuraiana	Rw2G040600 Rw5G012490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	645
AccB1I	GGYRCC	1 cut(s)	494
AccI	GTMKAC	2 cut(s)	468, 579
AciI	CCGC	1 cut(s)	98
AclWI	GGATC	5 cut(s)	260, 281, 425, 740, 974
AcoI	YGGCCR	1 cut(s)	541
AcsI	RAATTY	2 cut(s)	418, 621
AfaI	GTAC	1 cut(s)	653
AfiI	CCNNNNNNNGG	2 cut(s)	539, 917
AflIII	ACRYGT	1 cut(s)	751
AgsI	TTSAA	6 cut(s)	628, 667, 702, 779, 788, 897
AjiI	CACGTC	1 cut(s)	754
AjnI	CCWGG	1 cut(s)	148
AleI	CACNNNNGTG	1 cut(s)	439
AluBI	AGCT	9 cut(s)	28, 181, 290, 599, 720, 748, 900, 952, 970
AluI	AGCT	9 cut(s)	28, 181, 290, 599, 720, 748, 900, 952, 970
Alw26I	GTCTC	2 cut(s)	764, 949
AlwI	GGATC	5 cut(s)	260, 281, 425, 740, 974
AlwNI	CAGNNNCTG	1 cut(s)	380
Ama87I	CYCGRG	2 cut(s)	437, 910
AoxI	GGCC	3 cut(s)	448, 541, 570
ApeKI	GCWGC	2 cut(s)	294, 717
ApoI	RAATTY	2 cut(s)	418, 621
ArsI	GACNNNNNNTTYG	2 cut(s)	756, 788
AspLEI	GCGC	1 cut(s)	35
AspS9I	GGNCC	1 cut(s)	856
AsuHPI	GGTGA	2 cut(s)	100, 112
AsuII	TTCGAA	1 cut(s)	723
AsuNHI	GCTAGC	1 cut(s)	290
AvaI	CYCGRG	2 cut(s)	437, 910
AvaII	GGWCC	1 cut(s)	856
BalI	TGGCCA	1 cut(s)	543
BanI	GGYRCC	1 cut(s)	494
BbvCI	CCTCAGC	1 cut(s)	600
BbvI	GCAGC	2 cut(s)	306, 729
BccI	CCATC	2 cut(s)	646, 991
BceAI	ACGGC	1 cut(s)	934
BciT130I	CCWGG	1 cut(s)	150
BcoDI	GTCTC	2 cut(s)	764, 949
BfaI	CTAG	1 cut(s)	291
BfmI	CTRYAG	2 cut(s)	739, 1003
BisI	GCNGC	2 cut(s)	295, 718
BlpI	GCTNAGC	1 cut(s)	29
BlsI	GCNGC	2 cut(s)	296, 719
Bme1390I	CCNGG	1 cut(s)	150
Bme18I	GGWCC	1 cut(s)	856
BmeT110I	CYCGRG	2 cut(s)	437, 910
BmgBI	CACGTC	1 cut(s)	754
BmgT120I	GGNCC	1 cut(s)	856
BmiI	GGNNCC	3 cut(s)	496, 858, 908
BmrFI	CCNGG	1 cut(s)	150
BmrI	ACTGGG	2 cut(s)	408, 952
BmsI	GCATC	1 cut(s)	132
BmtI	GCTAGC	1 cut(s)	294
BmuI	ACTGGG	2 cut(s)	408, 952
Bpu10I	CCTNAGC	1 cut(s)	600
Bpu1102I	GCTNAGC	1 cut(s)	29
Bpu14I	TTCGAA	1 cut(s)	723
BpuEI	CTTGAG	1 cut(s)	437
Bsa29I	ATCGAT	1 cut(s)	276
BsaBI	GATNNNNATC	2 cut(s)	109, 251
BsaI	GGTCTC	2 cut(s)	764, 949
BsaJI	CCNNGG	4 cut(s)	149, 269, 475, 544
Bsc4I	CCNNNNNNNGG	2 cut(s)	539, 917
Bse1I	ACTGG	4 cut(s)	403, 842, 947, 959
Bse8I	GATNNNNATC	2 cut(s)	109, 251
BseBI	CCWGG	1 cut(s)	150
BseCI	ATCGAT	1 cut(s)	276
BseDI	CCNNGG	4 cut(s)	149, 269, 475, 544
BseGI	GGATG	4 cut(s)	133, 516, 817, 952
BseJI	GATNNNNATC	2 cut(s)	109, 251
BseLI	CCNNNNNNNGG	2 cut(s)	539, 917
BseMII	CTCAG	2 cut(s)	591, 944
BseNI	ACTGG	4 cut(s)	403, 842, 947, 959
BseRI	GAGGAG	1 cut(s)	191
BseXI	GCAGC	2 cut(s)	306, 729
BshFI	GGCC	3 cut(s)	450, 543, 572
BshNI	GGYRCC	1 cut(s)	494
BshVI	ATCGAT	1 cut(s)	276
BsiHKCI	CYCGRG	2 cut(s)	437, 910
BslFI	GGGAC	2 cut(s)	317, 973
BslI	CCNNNNNNNGG	2 cut(s)	539, 917
BsmAI	GTCTC	2 cut(s)	764, 949
BsmFI	GGGAC	2 cut(s)	317, 973
BsnI	GGCC	3 cut(s)	450, 543, 572
Bso31I	GGTCTC	2 cut(s)	764, 949
BsoBI	CYCGRG	2 cut(s)	437, 910
Bsp119I	TTCGAA	1 cut(s)	723
Bsp143I	GATC	7 cut(s)	265, 273, 277, 430, 732, 791, 979
Bsp1720I	GCTNAGC	1 cut(s)	29
Bsp19I	CCATGG	1 cut(s)	544
BspACI	CCGC	1 cut(s)	98
BspANI	GGCC	3 cut(s)	450, 543, 572
BspCNI	CTCAG	2 cut(s)	592, 945
BspDI	ATCGAT	1 cut(s)	276
BspLI	GGNNCC	3 cut(s)	496, 858, 908
BspMAI	CTGCAG	1 cut(s)	743
BspOI	GCTAGC	1 cut(s)	294
BspPI	GGATC	5 cut(s)	260, 281, 425, 740, 974
BspT104I	TTCGAA	1 cut(s)	723
BspT107I	GGYRCC	1 cut(s)	494
BspTNI	GGTCTC	2 cut(s)	764, 949
BsrI	ACTGG	4 cut(s)	403, 842, 947, 959
BssECI	CCNNGG	4 cut(s)	149, 269, 475, 544
BssMI	GATC	7 cut(s)	265, 273, 277, 430, 732, 791, 979
BssT1I	CCWWGG	2 cut(s)	269, 544
Bst2UI	CCWGG	1 cut(s)	150
Bst4CI	ACNGT	3 cut(s)	353, 476, 1004
Bst6I	CTCTTC	4 cut(s)	12, 126, 209, 325
BstBI	TTCGAA	1 cut(s)	723
BstC8I	GCNNGC	3 cut(s)	292, 487, 972
BstDEI	CTNAG	3 cut(s)	29, 600, 953
BstDSI	CCRYGG	2 cut(s)	475, 544
BstF5I	GGATG	4 cut(s)	133, 516, 817, 952
BstHHI	GCGC	1 cut(s)	35
BstKTI	GATC	7 cut(s)	268, 276, 280, 433, 735, 794, 982
BstMAI	GTCTC	2 cut(s)	764, 949
BstMBI	GATC	7 cut(s)	265, 273, 277, 430, 732, 791, 979
BstNI	CCWGG	1 cut(s)	150
BstNSI	RCATGY	2 cut(s)	8, 717
BstSCI	CCNGG	1 cut(s)	148
BstSFI	CTRYAG	2 cut(s)	739, 1003
BstV1I	GCAGC	2 cut(s)	306, 729
BstX2I	RGATCY	2 cut(s)	430, 732
BstXI	CCANNNNNNTGG	1 cut(s)	229
BstYI	RGATCY	2 cut(s)	430, 732
Bsu15I	ATCGAT	1 cut(s)	276
BsuRI	GGCC	3 cut(s)	450, 543, 572
BsuTUI	ATCGAT	1 cut(s)	276
BtgI	CCRYGG	2 cut(s)	475, 544
BtrI	CACGTC	1 cut(s)	754
BtsCI	GGATG	4 cut(s)	133, 516, 817, 952
BtsI	GCAGTG	1 cut(s)	748
BtsIMutI	CAGTG	5 cut(s)	358, 396, 748, 835, 966
Cac8I	GCNNGC	3 cut(s)	292, 487, 972
CaiI	CAGNNNCTG	1 cut(s)	380
CfoI	GCGC	1 cut(s)	35
Cfr13I	GGNCC	1 cut(s)	856
ClaI	ATCGAT	1 cut(s)	276
Csp6I	GTAC	1 cut(s)	652
CspCI	CAANNNNNGTGG	4 cut(s)	385, 420, 789, 824
CviAII	CATG	4 cut(s)	5, 545, 714, 806
CviQI	GTAC	1 cut(s)	652
DdeI	CTNAG	3 cut(s)	29, 600, 953
DpnI	GATC	7 cut(s)	267, 275, 279, 432, 734, 793, 981
DpnII	GATC	7 cut(s)	265, 273, 277, 430, 732, 791, 979
EaeI	YGGCCR	1 cut(s)	541
Eam1104I	CTCTTC	4 cut(s)	12, 126, 209, 325
EarI	CTCTTC	4 cut(s)	12, 126, 209, 325
Eco130I	CCWWGG	2 cut(s)	269, 544
Eco31I	GGTCTC	2 cut(s)	764, 949
Eco32I	GATATC	1 cut(s)	253
Eco47I	GGWCC	1 cut(s)	856
Eco88I	CYCGRG	2 cut(s)	437, 910
EcoRII	CCWGG	1 cut(s)	148
EcoRV	GATATC	1 cut(s)	253
EcoT14I	CCWWGG	2 cut(s)	269, 544
EcoT22I	ATGCAT	1 cut(s)	6
ErhI	CCWWGG	2 cut(s)	269, 544
FaeI	CATG	4 cut(s)	8, 548, 717, 809
FalI	AAGNNNNNCTT	2 cut(s)	129, 161
FaqI	GGGAC	2 cut(s)	317, 973
FatI	CATG	4 cut(s)	4, 544, 713, 805
FblI	GTMKAC	2 cut(s)	468, 579
Fnu4HI	GCNGC	2 cut(s)	295, 718
FokI	GGATG	4 cut(s)	140, 523, 824, 959
Fsp4HI	GCNGC	2 cut(s)	295, 718
FspBI	CTAG	1 cut(s)	291
GlaI	GCGC	1 cut(s)	34
GluI	GCNGC	2 cut(s)	295, 718
HaeIII	GGCC	3 cut(s)	450, 543, 572
HhaI	GCGC	1 cut(s)	35
Hin1II	CATG	4 cut(s)	8, 548, 717, 809
Hin6I	GCGC	1 cut(s)	33
HinP1I	GCGC	1 cut(s)	33
HincII	GTYRAC	2 cut(s)	580, 823
HindII	GTYRAC	2 cut(s)	580, 823
HindIII	AAGCTT	1 cut(s)	898
HinfI	GANTC	6 cut(s)	369, 633, 671, 698, 725, 767
HphI	GGTGA	2 cut(s)	100, 112
Hpy166II	GTNNAC	5 cut(s)	12, 469, 580, 654, 823
Hpy188I	TCNGA	1 cut(s)	115
Hpy188III	TCNNGA	4 cut(s)	381, 454, 607, 761
Hpy8I	GTNNAC	5 cut(s)	12, 469, 580, 654, 823
Hpy99I	CGWCG	3 cut(s)	584, 758, 920
HpyAV	CCTTC	2 cut(s)	199, 539
HpyCH4III	ACNGT	3 cut(s)	353, 476, 1004
HpyCH4IV	ACGT	2 cut(s)	582, 753
HpyCH4V	TGCA	6 cut(s)	4, 327, 444, 717, 741, 884
HpyF3I	CTNAG	3 cut(s)	29, 600, 953
HpySE526I	ACGT	2 cut(s)	582, 753
Hsp92II	CATG	4 cut(s)	8, 548, 717, 809
HspAI	GCGC	1 cut(s)	33
Kzo9I	GATC	7 cut(s)	265, 273, 277, 430, 732, 791, 979
LmnI	GCTCC	1 cut(s)	178
Lsp1109I	GCAGC	2 cut(s)	306, 729
LweI	GCATC	1 cut(s)	132
MaeI	CTAG	1 cut(s)	291
MaeII	ACGT	2 cut(s)	582, 753
MaeIII	GTNAC	3 cut(s)	88, 370, 583
MalI	GATC	7 cut(s)	267, 275, 279, 432, 734, 793, 981
MboI	GATC	7 cut(s)	265, 273, 277, 430, 732, 791, 979
MboII	GAAGA	7 cut(s)	29, 143, 226, 342, 348, 605, 776
MflI	RGATCY	2 cut(s)	430, 732
MlsI	TGGCCA	1 cut(s)	543
MluCI	AATT	4 cut(s)	51, 418, 621, 801
MluNI	TGGCCA	1 cut(s)	543
MlyI	GAGTC	1 cut(s)	378
MmeI	TCCRAC	2 cut(s)	191, 834
Mox20I	TGGCCA	1 cut(s)	543
Mph1103I	ATGCAT	1 cut(s)	6
MscI	TGGCCA	1 cut(s)	543
MseI	TTAA	1 cut(s)	426
MslI	CAYNNNNRTG	3 cut(s)	227, 439, 810
Msp20I	TGGCCA	1 cut(s)	543
MspA1I	CMGCKG	1 cut(s)	970
MspR9I	CCNGG	1 cut(s)	150
MvaI	CCWGG	1 cut(s)	150
NcoI	CCATGG	1 cut(s)	544
NdeII	GATC	7 cut(s)	265, 273, 277, 430, 732, 791, 979
NheI	GCTAGC	1 cut(s)	290
NlaIII	CATG	4 cut(s)	8, 548, 717, 809
NlaIV	GGNNCC	3 cut(s)	496, 858, 908
NmuCI	GTSAC	2 cut(s)	88, 370
NsiI	ATGCAT	1 cut(s)	6
NspI	RCATGY	2 cut(s)	8, 717
NspV	TTCGAA	1 cut(s)	723
OliI	CACNNNNGTG	1 cut(s)	439
PaeR7I	CTCGAG	1 cut(s)	437
PcsI	WCGNNNNNNNCGW	1 cut(s)	759
PfeI	GAWTC	5 cut(s)	633, 671, 698, 725, 767
PkrI	GCNGC	2 cut(s)	296, 719
PleI	GAGTC	1 cut(s)	377
PpsI	GAGTC	1 cut(s)	377
PsiI	TTATAA	1 cut(s)	645
Psp6I	CCWGG	1 cut(s)	148
PspGI	CCWGG	1 cut(s)	148
PspN4I	GGNNCC	3 cut(s)	496, 858, 908
PspPI	GGNCC	1 cut(s)	856
PspXI	VCTCGAGB	1 cut(s)	437
PstI	CTGCAG	1 cut(s)	743
PstNI	CAGNNNCTG	1 cut(s)	380
PsuI	RGATCY	2 cut(s)	430, 732
PvuII	CAGCTG	1 cut(s)	970
RsaI	GTAC	1 cut(s)	653
RsaNI	GTAC	1 cut(s)	652
RseI	CAYNNNNRTG	3 cut(s)	227, 439, 810
SalI	GTCGAC	1 cut(s)	578
SaqAI	TTAA	1 cut(s)	426
SatI	GCNGC	2 cut(s)	295, 718
Sau3AI	GATC	7 cut(s)	265, 273, 277, 430, 732, 791, 979
Sau96I	GGNCC	1 cut(s)	856
SchI	GAGTC	1 cut(s)	378
ScrFI	CCNGG	1 cut(s)	150
SfaNI	GCATC	1 cut(s)	132
SfcI	CTRYAG	2 cut(s)	739, 1003
Sfr274I	CTCGAG	1 cut(s)	437
SfuI	TTCGAA	1 cut(s)	723
SinI	GGWCC	1 cut(s)	856
SlaI	CTCGAG	1 cut(s)	437
SmiMI	CAYNNNNRTG	3 cut(s)	227, 439, 810
SmlI	CTYRAG	2 cut(s)	437, 452
SmoI	CTYRAG	2 cut(s)	437, 452
Sse9I	AATT	4 cut(s)	51, 418, 621, 801
SsiI	CCGC	1 cut(s)	98
SspMI	CTAG	1 cut(s)	291
StyD4I	CCNGG	1 cut(s)	148
StyI	CCWWGG	2 cut(s)	269, 544
TaaI	ACNGT	3 cut(s)	353, 476, 1004
TaiI	ACGT	2 cut(s)	585, 756
TaqI	TCGA	5 cut(s)	276, 438, 579, 723, 762
TaqII	GACCGA	2 cut(s)	746, 760
TasI	AATT	4 cut(s)	51, 418, 621, 801
TatI	WGTACW	1 cut(s)	651
TfiI	GAWTC	5 cut(s)	633, 671, 698, 725, 767
Tru1I	TTAA	1 cut(s)	426
Tru9I	TTAA	1 cut(s)	426
TscAI	CASTG	5 cut(s)	358, 403, 748, 842, 966
TseFI	GTSAC	2 cut(s)	88, 370
TseI	GCWGC	2 cut(s)	294, 717
Tsp45I	GTSAC	2 cut(s)	88, 370
TspDTI	ATGAA	5 cut(s)	99, 144, 530, 794, 828
TspRI	CASTG	5 cut(s)	358, 403, 748, 842, 966
VpaK11BI	GGWCC	1 cut(s)	856
XapI	RAATTY	2 cut(s)	418, 621
XceI	RCATGY	2 cut(s)	8, 717
XhoI	CTCGAG	1 cut(s)	437
XmiI	GTMKAC	2 cut(s)	468, 579
XspI	CTAG	1 cut(s)	291
Zsp2I	ATGCAT	1 cut(s)	6

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.