pycom10g08370

RNA pseudouridine synthase 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0001594
Physical Location & Seq
Reverse (-)
10891376 .. 10894596
3221 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1461 bp
ATGGTGGGCCCCATACTCCTCCGCCGACTCCTCCTCCCCCGTCCACCACCGTCCTCCGCCGAGTTCGGCAGCGCGAGAATATGCTTCTCAGCCTTCTACAACTGCCGATCTTACGCCGCCACCTCTGAAACAATTGACGAAGAGAAAGAAAAGGGCGGTCAAAAATGGTTCACTTTACCTTCGTTCACTGCCGCTGTAAACGGCGCCGCTGTGGGTAAAGAGCTCTCTCGCAATCGTTCAAACGGCGGCAGAGGTACAATCACAAATACTGCCTCTGCCGCCGCCGACACCACCACCACGACGGCGCTTAAATGGGTTACTCGGTGCTGCCCGGAGCTTCCTAGAAGCCTCGTTCAGAAGCTCTTTCGCCTTAGACAGGTTCGAAGAGAATCATGTTCTGGTCAAGCTGTTGAAGCACATGAGCGCCCACTTAAAAGGGTGGCGGCTAAAGATTCCATGAACATAGGAGATCGAATACATCTTCCTTTTAGCGTGCAAGAGTTCCCTGCTGACAAACACAAGTCGTCCAATTATAAACAAGAATGTCATTGTAGTGAAGAAGAAATCAACTTTATCCGGAGCCTGGAGTTGTATAAGGATCCAGCCATCATAGTCATCAATAAACCCCCTGGAATGCCTGTTCAGGGTGGGATTGGAATCAAACGAAGTCTAGATAAACTGGCTACCGCTTGTTTGAGTTATGACTTGTCAGAACCCCCTCGGCTGGTACATAGACTTGACCGAGATAGTAGTGGTCTCCTGGTGATGGGAAGGACACAAACAATTGCGGCTGTTCTGCATTCCATCTTCCGTGAAAAAACTGTTGGAGCATCAAATGATGTAACTGACGATGCAAAAAGAATTCTGCAAAGAAGGTATTGGGCACTTGTCATTGGATCTCCCAGGCAACGTAAAGGATTAATCTCAGCACCTCTGGTAAAGGTGGTGGTGGACAATGGTAAATCGGAGAGAATCACAGCTGCAAACGATTCACAAACTTTGTCTTCTCAGCATGCCATTACAAAGTACAAAGTGATTGGATCATCATGTCCTGGTTTCACATGGCTAGAGCTAACCCCTCTTACTGGCCGAAAGCATCAGCTGCGTGTACACTGTGCGGAGGTTTTGAGAACACCGATAGTTGGAGACTACAAATACGGGTGGCAAACTCATAGGAACTGGAAACCTTTATCTTCGTCTAATACTGAATCTCATTGGAATGAGGAGCTTCGCCCAAGAAGAAACCTCCCCTTTGATCTGAATATGGACAGTGGAAGTATCTCCGAAAAGACACCCCGCTTGCATCTTCATTGTAAGCAAATGGTTTTGCCTAATGTGTATCTGGCTTTGCAAAACGTGAAGCTGCATTCAGATTGTGATCTTTCTGAACTAGAAAGCCTAGAGTTGGTTGCTCCTCTGCCTTCATACATGCAAAGAAGTTGGGATATTACAAATTATTAA

Protein Analysis

487

Amino Acids

54.44

Weight (kDa)

9.41

Isoelectric Point (pI)

54.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PseudoU_synth_2 PF00849 203 - 373 2.2e-27 RNA pseudouridylate synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 534
AccB1I GGYRCC 1 cut(s) 203
AccII CGCG 1 cut(s) 74
AccIII TCCGGA 1 cut(s) 576
AclWI GGATC 4 cut(s) 593, 606, 904, 1048
AcoI YGGCCR 1 cut(s) 1087
AcsI RAATTY 1 cut(s) 861
AcyI GRCGYC 1 cut(s) 204
AfaI GTAC 4 cut(s) 256, 729, 1028, 1110
AfiI CCNNNNNNNGG 8 cut(s) 376, 583, 644, 724, 766, 1085, 1142, 1405
AgsI TTSAA 2 cut(s) 240, 413
AjnI CCWGG 5 cut(s) 582, 628, 759, 902, 1051
AjuI GAANNNNNNNTTGG 2 cut(s) 807, 839
AloI GAACNNNNNNTCC 2 cut(s) 624, 656
AluBI AGCT 9 cut(s) 223, 337, 361, 407, 980, 1072, 1102, 1228, 1363
AluI AGCT 9 cut(s) 223, 337, 361, 407, 980, 1072, 1102, 1228, 1363
Alw21I GWGCWC 1 cut(s) 225
Alw26I GTCTC 2 cut(s) 761, 1140
AlwI GGATC 4 cut(s) 593, 606, 904, 1048
Aor13HI TCCGGA 1 cut(s) 576
AoxI GGCC 2 cut(s) 7, 1087
ApaI GGGCCC 1 cut(s) 11
ApeKI GCWGC 5 cut(s) 69, 327, 980, 1102, 1363
ApoI RAATTY 1 cut(s) 861
AseI ATTAAT 1 cut(s) 920
AspLEI GCGC 4 cut(s) 74, 206, 307, 426
AspS9I GGNCC 2 cut(s) 7, 8
AsuC2I CCSGG 1 cut(s) 332
AsuHPI GGTGA 1 cut(s) 775
AsuII TTCGAA 1 cut(s) 382
BaeGI GKGCMC 2 cut(s) 11, 886
BamHI GGATCC 1 cut(s) 598
BanI GGYRCC 1 cut(s) 203
BanII GRGCYC 2 cut(s) 11, 225
BbsI GAAGAC 1 cut(s) 996
Bbv12I GWGCWC 1 cut(s) 225
BbvI GCAGC 5 cut(s) 81, 314, 967, 1089, 1350
BccI CCATC 3 cut(s) 614, 760, 812
BceAI ACGGC 3 cut(s) 217, 259, 318
BciT130I CCWGG 5 cut(s) 584, 630, 761, 904, 1053
BcnI CCSGG 1 cut(s) 332
BcoDI GTCTC 2 cut(s) 761, 1140
BfaI CTAG 5 cut(s) 342, 671, 1067, 1391, 1400
BfoI RGCGCY 3 cut(s) 207, 308, 427
Bme1390I CCNGG 6 cut(s) 332, 584, 630, 761, 904, 1053
BmgT120I GGNCC 2 cut(s) 7, 8
BmiI GGNNCC 5 cut(s) 9, 10, 205, 581, 600
BmrFI CCNGG 6 cut(s) 332, 584, 630, 761, 904, 1053
BmsI GCATC 4 cut(s) 839, 841, 1105, 1312
BpiI GAAGAC 1 cut(s) 996
BpmI CTGGAG 1 cut(s) 605
Bpu14I TTCGAA 1 cut(s) 382
BpuMI CCSGG 1 cut(s) 332
BsaBI GATNNNNATC 2 cut(s) 656, 1377
BsaHI GRCGYC 1 cut(s) 204
BsaI GGTCTC 1 cut(s) 761
BsaJI CCNNGG 3 cut(s) 628, 719, 902
BsaWI WCCGGW 1 cut(s) 576
BsaXI ACNNNNNCTCC 2 cut(s) 18, 48
Bsc4I CCNNNNNNNGG 8 cut(s) 376, 583, 644, 724, 766, 1085, 1142, 1405
Bse1I ACTGG 3 cut(s) 684, 1090, 1184
Bse8I GATNNNNATC 2 cut(s) 656, 1377
BseAI TCCGGA 1 cut(s) 576
BseBI CCWGG 5 cut(s) 584, 630, 761, 904, 1053
BseDI CCNNGG 3 cut(s) 628, 719, 902
BseJI GATNNNNATC 2 cut(s) 656, 1377
BseLI CCNNNNNNNGG 8 cut(s) 376, 583, 644, 724, 766, 1085, 1142, 1405
BseMII CTCAG 3 cut(s) 102, 939, 1022
BseNI ACTGG 3 cut(s) 684, 1090, 1184
BseRI GAGGAG 5 cut(s) 8, 20, 23, 1238, 1404
BseSI GKGCMC 2 cut(s) 11, 886
BseXI GCAGC 5 cut(s) 81, 314, 967, 1089, 1350
Bsh1236I CGCG 1 cut(s) 74
BshFI GGCC 2 cut(s) 9, 1089
BshNI GGYRCC 1 cut(s) 203
BsiHKAI GWGCWC 1 cut(s) 225
BsiSI CCGG 2 cut(s) 332, 577
BslI CCNNNNNNNGG 8 cut(s) 376, 583, 644, 724, 766, 1085, 1142, 1405
BsmAI GTCTC 2 cut(s) 761, 1140
BsmI GAATGC 3 cut(s) 639, 799, 1366
BsnI GGCC 2 cut(s) 9, 1089
Bso31I GGTCTC 1 cut(s) 761
Bsp119I TTCGAA 1 cut(s) 382
Bsp120I GGGCCC 1 cut(s) 7
Bsp1286I GDGCHC 3 cut(s) 11, 225, 886
Bsp13I TCCGGA 1 cut(s) 576
Bsp1407I TGTACA 1 cut(s) 1108
Bsp143I GATC 7 cut(s) 107, 469, 598, 896, 1040, 1255, 1378
BspANI GGCC 2 cut(s) 9, 1089
BspCNI CTCAG 3 cut(s) 101, 938, 1021
BspEI TCCGGA 1 cut(s) 576
BspFNI CGCG 1 cut(s) 74
BspLI GGNNCC 5 cut(s) 9, 10, 205, 581, 600
BspPI GGATC 4 cut(s) 593, 606, 904, 1048
BspT104I TTCGAA 1 cut(s) 382
BspT107I GGYRCC 1 cut(s) 203
BspTNI GGTCTC 1 cut(s) 761
BsrGI TGTACA 1 cut(s) 1108
BsrI ACTGG 3 cut(s) 684, 1090, 1184
BssECI CCNNGG 3 cut(s) 628, 719, 902
BssMI GATC 7 cut(s) 107, 469, 598, 896, 1040, 1255, 1378
BssNI GRCGYC 1 cut(s) 204
Bst2UI CCWGG 5 cut(s) 584, 630, 761, 904, 1053
Bst4CI ACNGT 4 cut(s) 51, 823, 1115, 1271
Bst6I CTCTTC 2 cut(s) 135, 379
BstACI GRCGYC 1 cut(s) 204
BstAPI GCANNNNNTGC 1 cut(s) 1102
BstAUI TGTACA 1 cut(s) 1108
BstBI TTCGAA 1 cut(s) 382
BstC8I GCNNGC 3 cut(s) 494, 1014, 1301
BstDEI CTNAG 4 cut(s) 88, 371, 925, 1008
BstENI CCTNNNNNAGG 1 cut(s) 374
BstFNI CGCG 1 cut(s) 74
BstH2I RGCGCY 3 cut(s) 207, 308, 427
BstHHI GCGC 4 cut(s) 74, 206, 307, 426
BstKTI GATC 7 cut(s) 110, 472, 601, 899, 1043, 1258, 1381
BstMAI GTCTC 2 cut(s) 761, 1140
BstMBI GATC 7 cut(s) 107, 469, 598, 896, 1040, 1255, 1378
BstMWI GCNNNNNNNGC 3 cut(s) 278, 413, 1102
BstNI CCWGG 5 cut(s) 584, 630, 761, 904, 1053
BstNSI RCATGY 2 cut(s) 1016, 1432
BstSCI CCNGG 6 cut(s) 330, 582, 628, 759, 902, 1051
BstSLI GKGCMC 2 cut(s) 11, 886
BstUI CGCG 1 cut(s) 74
BstV1I GCAGC 5 cut(s) 81, 314, 967, 1089, 1350
BstV2I GAAGAC 1 cut(s) 996
BstX2I RGATCY 2 cut(s) 598, 896
BstYI RGATCY 2 cut(s) 598, 896
BsuRI GGCC 2 cut(s) 9, 1089
BtsI GCAGTG 1 cut(s) 186
BtsIMutI CAGTG 3 cut(s) 186, 1111, 1276
Cac8I GCNNGC 3 cut(s) 494, 1014, 1301
CfoI GCGC 4 cut(s) 74, 206, 307, 426
Cfr13I GGNCC 2 cut(s) 7, 8
Csp6I GTAC 4 cut(s) 255, 728, 1027, 1109
CviAII CATG 7 cut(s) 393, 419, 457, 1013, 1047, 1062, 1429
CviQI GTAC 4 cut(s) 255, 728, 1027, 1109
DdeI CTNAG 4 cut(s) 88, 371, 925, 1008
DinI GGCGCC 1 cut(s) 205
DpnI GATC 7 cut(s) 109, 471, 600, 898, 1042, 1257, 1380
DpnII GATC 7 cut(s) 107, 469, 598, 896, 1040, 1255, 1378
EaeI YGGCCR 1 cut(s) 1087
Eam1104I CTCTTC 2 cut(s) 135, 379
EarI CTCTTC 2 cut(s) 135, 379
EciI GGCGGA 2 cut(s) 11, 46
Ecl136II GAGCTC 1 cut(s) 223
Eco24I GRGCYC 2 cut(s) 11, 225
Eco31I GGTCTC 1 cut(s) 761
Eco53kI GAGCTC 1 cut(s) 223
EcoICRI GAGCTC 1 cut(s) 223
EcoNI CCTNNNNNAGG 1 cut(s) 374
EcoO109I RGGNCCY 1 cut(s) 8
EcoRI GAATTC 1 cut(s) 861
EcoRII CCWGG 5 cut(s) 582, 628, 759, 902, 1051
EcoT38I GRGCYC 2 cut(s) 11, 225
EgeI GGCGCC 1 cut(s) 205
EheI GGCGCC 1 cut(s) 205
FaeI CATG 7 cut(s) 396, 422, 460, 1016, 1050, 1065, 1432
FatI CATG 7 cut(s) 392, 418, 456, 1012, 1046, 1061, 1428
FauI CCCGC 1 cut(s) 1304
FriOI GRGCYC 2 cut(s) 11, 225
FspBI CTAG 5 cut(s) 342, 671, 1067, 1391, 1400
GlaI GCGC 4 cut(s) 73, 205, 306, 425
GsuI CTGGAG 1 cut(s) 605
HaeII RGCGCY 3 cut(s) 207, 308, 427
HaeIII GGCC 2 cut(s) 9, 1089
HapII CCGG 2 cut(s) 332, 577
HhaI GCGC 4 cut(s) 74, 206, 307, 426
Hin1I GRCGYC 1 cut(s) 204
Hin1II CATG 7 cut(s) 396, 422, 460, 1016, 1050, 1065, 1432
Hin6I GCGC 4 cut(s) 72, 204, 305, 424
HinP1I GCGC 4 cut(s) 72, 204, 305, 424
HinfI GANTC 7 cut(s) 27, 389, 452, 657, 972, 989, 1208
HpaII CCGG 2 cut(s) 332, 577
HphI GGTGA 1 cut(s) 775
Hpy166II GTNNAC 7 cut(s) 44, 171, 186, 199, 952, 1109, 1111
Hpy188I TCNGA 8 cut(s) 127, 357, 712, 967, 1260, 1285, 1372, 1387
Hpy188III TCNNGA 2 cut(s) 577, 671
Hpy8I GTNNAC 7 cut(s) 44, 171, 186, 199, 952, 1109, 1111
Hpy99I CGWCG 1 cut(s) 304
HpyAV CCTTC 5 cut(s) 103, 189, 765, 867, 1431
HpyCH4III ACNGT 4 cut(s) 51, 823, 1115, 1271
HpyCH4IV ACGT 2 cut(s) 910, 1356
HpyCH4V TGCA 9 cut(s) 496, 799, 854, 868, 983, 1303, 1351, 1366, 1432
HpyF10VI GCNNNNNNNGC 3 cut(s) 278, 413, 1102
HpyF3I CTNAG 4 cut(s) 88, 371, 925, 1008
HpySE526I ACGT 2 cut(s) 910, 1356
Hsp92I GRCGYC 1 cut(s) 204
Hsp92II CATG 7 cut(s) 396, 422, 460, 1016, 1050, 1065, 1432
HspAI GCGC 4 cut(s) 72, 204, 305, 424
KasI GGCGCC 1 cut(s) 203
Kpn2I TCCGGA 1 cut(s) 576
Kzo9I GATC 7 cut(s) 107, 469, 598, 896, 1040, 1255, 1378
LmnI GCTCC 5 cut(s) 334, 579, 827, 1225, 1417
Lsp1109I GCAGC 5 cut(s) 81, 314, 967, 1089, 1350
LweI GCATC 4 cut(s) 839, 841, 1105, 1312
MaeI CTAG 5 cut(s) 342, 671, 1067, 1391, 1400
MaeII ACGT 2 cut(s) 910, 1356
MaeIII GTNAC 2 cut(s) 316, 841
MalI GATC 7 cut(s) 109, 471, 600, 898, 1042, 1257, 1380
MboI GATC 7 cut(s) 107, 469, 598, 896, 1040, 1255, 1378
MfeI CAATTG 2 cut(s) 132, 783
MflI RGATCY 2 cut(s) 598, 896
MhlI GDGCHC 3 cut(s) 11, 225, 886
MluCI AATT 5 cut(s) 132, 529, 783, 861, 1453
Mly113I GGCGCC 1 cut(s) 204
MlyI GAGTC 1 cut(s) 21
MmeI TCCRAC 2 cut(s) 805, 1123
MroI TCCGGA 1 cut(s) 576
MseI TTAA 4 cut(s) 309, 432, 920, 1459
MslI CAYNNNNRTG 2 cut(s) 552, 1218
MspA1I CMGCKG 4 cut(s) 194, 209, 980, 1102
MspI CCGG 2 cut(s) 332, 577
MspR9I CCNGG 6 cut(s) 332, 584, 630, 761, 904, 1053
MunI CAATTG 2 cut(s) 132, 783
Mva1269I GAATGC 3 cut(s) 639, 799, 1366
MvaI CCWGG 5 cut(s) 584, 630, 761, 904, 1053
MvnI CGCG 1 cut(s) 74
MwoI GCNNNNNNNGC 3 cut(s) 278, 413, 1102
NarI GGCGCC 1 cut(s) 204
NciI CCSGG 1 cut(s) 332
NdeII GATC 7 cut(s) 107, 469, 598, 896, 1040, 1255, 1378
NlaIII CATG 7 cut(s) 396, 422, 460, 1016, 1050, 1065, 1432
NlaIV GGNNCC 5 cut(s) 9, 10, 205, 581, 600
NmeAIII GCCGAG 2 cut(s) 85, 700
NspI RCATGY 2 cut(s) 1016, 1432
NspV TTCGAA 1 cut(s) 382
PaeI GCATGC 1 cut(s) 1016
PctI GAATGC 3 cut(s) 639, 799, 1366
PfeI GAWTC 6 cut(s) 389, 452, 657, 972, 989, 1208
PleI GAGTC 1 cut(s) 21
PluTI GGCGCC 1 cut(s) 207
PpsI GAGTC 1 cut(s) 21
PshBI ATTAAT 1 cut(s) 920
PsiI TTATAA 1 cut(s) 534
Psp124BI GAGCTC 1 cut(s) 225
Psp6I CCWGG 5 cut(s) 582, 628, 759, 902, 1051
PspGI CCWGG 5 cut(s) 582, 628, 759, 902, 1051
PspN4I GGNNCC 5 cut(s) 9, 10, 205, 581, 600
PspOMI GGGCCC 1 cut(s) 7
PspPI GGNCC 2 cut(s) 7, 8
PsuI RGATCY 2 cut(s) 598, 896
PvuII CAGCTG 2 cut(s) 980, 1102
RsaI GTAC 4 cut(s) 256, 729, 1028, 1110
RsaNI GTAC 4 cut(s) 255, 728, 1027, 1109
RseI CAYNNNNRTG 2 cut(s) 552, 1218
SacI GAGCTC 1 cut(s) 225
SaqAI TTAA 4 cut(s) 309, 432, 920, 1459
Sau3AI GATC 7 cut(s) 107, 469, 598, 896, 1040, 1255, 1378
Sau96I GGNCC 2 cut(s) 7, 8
SchI GAGTC 1 cut(s) 21
ScrFI CCNGG 6 cut(s) 332, 584, 630, 761, 904, 1053
SduI GDGCHC 3 cut(s) 11, 225, 886
SfaNI GCATC 4 cut(s) 839, 841, 1105, 1312
SfoI GGCGCC 1 cut(s) 205
SfuI TTCGAA 1 cut(s) 382
SmiMI CAYNNNNRTG 2 cut(s) 552, 1218
SphI GCATGC 1 cut(s) 1016
Sse9I AATT 5 cut(s) 132, 529, 783, 861, 1453
SspDI GGCGCC 1 cut(s) 203
SspMI CTAG 5 cut(s) 342, 671, 1067, 1391, 1400
SstI GAGCTC 1 cut(s) 225
StyD4I CCNGG 6 cut(s) 330, 582, 628, 759, 902, 1051
TaaI ACNGT 4 cut(s) 51, 823, 1115, 1271
TaiI ACGT 2 cut(s) 913, 1359
TaqI TCGA 2 cut(s) 382, 472
TaqII GACCGA 1 cut(s) 756
TasI AATT 5 cut(s) 132, 529, 783, 861, 1453
TatI WGTACW 2 cut(s) 1026, 1108
TauI GCSGC 8 cut(s) 119, 194, 209, 249, 281, 284, 446, 791
TfiI GAWTC 6 cut(s) 389, 452, 657, 972, 989, 1208
Tru1I TTAA 4 cut(s) 309, 432, 920, 1459
Tru9I TTAA 4 cut(s) 309, 432, 920, 1459
TscAI CASTG 3 cut(s) 193, 1118, 1276
TseI GCWGC 5 cut(s) 69, 327, 980, 1102, 1363
TspDTI ATGAA 3 cut(s) 473, 1298, 1413
TspGWI ACGGA 1 cut(s) 800
TspRI CASTG 3 cut(s) 193, 1118, 1276
VspI ATTAAT 1 cut(s) 920
XagI CCTNNNNNAGG 1 cut(s) 374
XapI RAATTY 1 cut(s) 861
XbaI TCTAGA 1 cut(s) 670
XceI RCATGY 2 cut(s) 1016, 1432
XspI CTAG 5 cut(s) 342, 671, 1067, 1391, 1400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.