Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0001594

Physical Location & Seq

Genomic Coordinates Forward (+)

10280967 .. 10284385

Gene Structure 3419 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G006410.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1422 bp

ATGCCGAATCACCTTCTCCTCCGCCGCATCCTCCTCCGTCCTCCACTCTCCTCCTCCGCCGAATCCGGCGGCGATAGGCTCTTCATCTCACCTTTCCACTACTCCCGATCTTACGCCACCACCCAACCATTTCACGAAGACAAACAAAAGCGCGGCAAAAAGTGGTTCACTCTACCTCCGTTCACTGACTCCGTAAACGGCGCCGCTTTGGGACGAGAGCTGTTTCGCAGCGTCGGCAGAGATGTGTATGCTAAACCCACAGCCGCCTCAGTCACGACGGCGCTTAAATGGGTCACCAGGTGCTGCCCTGAGATTCCCAGAAGCCTTGTCCAGAAGCTCTTTCGGTTAAGACAGGTTCGAAGAGAAGCATCTGACATGGATCAAGGTGTTCAAGCGCTTAAAAGGGTGGGGGCTAAAGACTCGATGAACTTAGGAGATCGAATTTGTCTTCCTATTTCTGTGAAACAGTTCCCTGATGATAAACAAGAAGAGGGACATTGTAGTGAAGAAGAAGTCAAGTTTATTTGTAGTCTCGTGTTGTATAAGGATCCAGCTATTGTTGTCATCAATAAACCCCCTGGATTGCCTGTTCAGGGCGGTATTGGAATCAAAAGGAGTTTAGATGAACTGGCTGCCACTTGTTTAAGTTACGGCTTTTCAGAACCCCCTCGTCTGGTACACAGACTTGACAGAGATTGTAGTGGTATCCTTGTGATGGGACGGACACAAATAAGTGCAACAGTTTTGCATTCCCTCTTCCGTGAGAAAACTTTTGGAGCTTCAGATGGTGTTATTGACCATGCTAAAGCCATTCTGCAAAGAAGGTATTGGGCACTCGTCATTGGATCTCCCAGACAACAAGAGGGGTTAATCTCCGTGCCTTTAGGAAAGGTGGTGGTAGACAATGGTAAATCAGATAGGATCACCATTGTGAGTAAGGCACAATGTTTGCCATCTCAACATGCACTTACAAAGTACAAAGTGATTGGATCATCATGTCCTGGTTTCACATGGTTAGAGCTAATTCCATTTACTGGTCGAAAGCACCAGCTGCGCGTACACTGTGCAGAAGTTTTGGGAACACCAATAGTCGGAGACTACAAATATGGATGGCGAACGCATAAGGACTGGAAACCTGTTGCTTGGTCTAAGATTGAAAGCCATTCCAGTGAGGGGCATTCGAAGAGAAAAAACATTCCTTTTGATCTCAATATGGACAGTGGAAGTATTGCCGAAAAGAATTTGCGCTTGCATCTTCATTCTAAGCAAATGGTTTTGCCTAATGTGTCTCAGGCTTTGCAAAATGTGAAACTGCATACGGAGTGTGATCTTTCAGGACTAGAAAGCCTAGAATTGGTTGCTCCTATGCCTTCATACATGCAAAAAAGTTGGGATATTACAAATTCTGGTTTTGATGGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001522 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0009451 GO:0009982 GO:0009987 GO:0010467 GO:0016070 GO:0016853 GO:0016866 GO:0031119 GO:0034470 GO:0034641 GO:0034660 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PseudoU_synth_2

Protein Analysis

473

Amino Acids

52.84

Weight (kDa)

9.42

Isoelectric Point (pI)

52.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PseudoU_synth_2	PF00849	186 - 356	3e-21	RNA pseudouridylate synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001594)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G19440 AT3G19440
fragaria_vesca	FvH4_2g05120 FvH4_2g05120 FvH4_2g05120 FvH4_2g05120
malus_domestica	MD10G1097600.v1.1
prunus_persica	Prupe.8G135400_v2.0.a1 Prupe.8G135400_v2.0.a1 Prupe.8G135400_v2.0.a1
pyrus_communis	pycom01g14220 pycom10g08370
rosa_chinensis	RchiOBHm_Chr4g0415551 RchiOBHm_Chr6g0255041
rosa_laevigata	RLG00000003460 RLG00000014859 RLG00000014868 RLG00000018864 RLG00000024043 RLG00000027684
rosa_multiflora	Rmu_ssc0000011.1_g000078
rosa_roxburghii	Rroxscaffold_2G00121110 Rroxscaffold_6G00396470 Rroxscaffold_6G00402590 Rroxscaffold_7G00210260
rosa_rugosa	Rorug03G0278700 Rorug03G0278800 Rorug03G0278900 Rorug05G0556400 Rorug05G0556500
rosa_samantha	Rh1AG091300 Rh1AG167900 Rh1DG094800 Rh2BG117700 Rh2BG330200 Rh2DG118600 Rh3BG349000 Rh4CG206700 Rh5BG449900 Rh6AG035800 Rh6AG073700 Rh6BG065900 Rh6BG066100 Rh6BG131700 Rh7AG357100 Rh7CG374500
rosa_wichuraiana	Rw2G034900 Rw6G006400 Rw6G006410 Rw6G026380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	200
AccB7I	CCANNNNNTGG	1 cut(s)	1034
AccI	GTMKAC	1 cut(s)	900
AccII	CGCG	2 cut(s)	153, 1056
AciI	CCGC	8 cut(s)	22, 25, 57, 69, 153, 204, 264, 597
AclWI	GGATC	6 cut(s)	387, 542, 555, 853, 929, 997
AcsI	RAATTY	3 cut(s)	441, 1240, 1402
AcuI	CTGAAG	1 cut(s)	765
AcyI	GRCGYC	1 cut(s)	201
AdeI	CACNNNGTG	1 cut(s)	300
AfaI	GTAC	3 cut(s)	678, 977, 1059
AfeI	AGCGCT	1 cut(s)	396
AfiI	CCNNNNNNNGG	7 cut(s)	593, 673, 715, 1034, 1091, 1173, 1354
AgsI	TTSAA	2 cut(s)	392, 1157
AjnI	CCWGG	3 cut(s)	296, 577, 1000
AleI	CACNNNNGTG	1 cut(s)	929
AloI	GAACNNNNNNTCC	2 cut(s)	573, 605
AluBI	AGCT	6 cut(s)	220, 337, 554, 779, 1021, 1051
AluI	AGCT	6 cut(s)	220, 337, 554, 779, 1021, 1051
Alw26I	GTCTC	3 cut(s)	536, 1089, 1293
AlwI	GGATC	6 cut(s)	387, 542, 555, 853, 929, 997
AlwNI	CAGNNNCTG	1 cut(s)	303
Aor51HI	AGCGCT	1 cut(s)	396
ApeKI	GCWGC	4 cut(s)	228, 303, 632, 1051
ApoI	RAATTY	3 cut(s)	441, 1240, 1402
Asp700I	GAANNNNTTC	1 cut(s)	467
AspLEI	GCGC	6 cut(s)	153, 203, 283, 397, 1056, 1248
AsuHPI	GGTGA	3 cut(s)	81, 286, 916
AsuII	TTCGAA	2 cut(s)	358, 1181
BaeGI	GKGCMC	1 cut(s)	835
BamHI	GGATCC	1 cut(s)	547
BanI	GGYRCC	1 cut(s)	200
BauI	CACGAG	1 cut(s)	533
BbsI	GAAGAC	2 cut(s)	144, 440
BbvI	GCAGC	4 cut(s)	240, 290, 619, 1038
BccI	CCATC	5 cut(s)	709, 779, 961, 1104, 1409
BceAI	ACGGC	3 cut(s)	214, 294, 667
BciT130I	CCWGG	3 cut(s)	298, 579, 1002
BciVI	GTATCC	1 cut(s)	716
BcoDI	GTCTC	3 cut(s)	536, 1089, 1293
BfaI	CTAG	2 cut(s)	1340, 1349
BfoI	RGCGCY	3 cut(s)	204, 284, 398
BfuI	GTATCC	1 cut(s)	716
BisI	GCNGC	9 cut(s)	25, 70, 154, 204, 229, 264, 304, 633, 1052
BlsI	GCNGC	9 cut(s)	26, 71, 155, 205, 230, 265, 305, 634, 1053
Bme1390I	CCNGG	3 cut(s)	298, 579, 1002
BmiI	GGNNCC	2 cut(s)	202, 549
BmrFI	CCNGG	3 cut(s)	298, 579, 1002
BmsI	GCATC	3 cut(s)	36, 377, 1261
BpiI	GAAGAC	2 cut(s)	144, 440
Bpu14I	TTCGAA	2 cut(s)	358, 1181
BsaHI	GRCGYC	1 cut(s)	201
BsaJI	CCNNGG	1 cut(s)	577
BsaXI	ACNNNNNCTCC	2 cut(s)	160, 190
Bsc4I	CCNNNNNNNGG	7 cut(s)	593, 673, 715, 1034, 1091, 1173, 1354
Bse1I	ACTGG	4 cut(s)	633, 1039, 1133, 1167
BseBI	CCWGG	3 cut(s)	298, 579, 1002
BseDI	CCNNGG	1 cut(s)	577
BseGI	GGATG	2 cut(s)	27, 1115
BseLI	CCNNNNNNNGG	7 cut(s)	593, 673, 715, 1034, 1091, 1173, 1354
BseMII	CTCAG	3 cut(s)	282, 300, 1304
BseNI	ACTGG	4 cut(s)	633, 1039, 1133, 1167
BseRI	GAGGAG	4 cut(s)	8, 23, 40, 43
BseSI	GKGCMC	1 cut(s)	835
BseXI	GCAGC	4 cut(s)	240, 290, 619, 1038
BsgI	GTGCAG	1 cut(s)	1086
Bsh1236I	CGCG	2 cut(s)	153, 1056
BshNI	GGYRCC	1 cut(s)	200
BsiSI	CCGG	1 cut(s)	66
BslFI	GGGAC	3 cut(s)	225, 507, 732
BslI	CCNNNNNNNGG	7 cut(s)	593, 673, 715, 1034, 1091, 1173, 1354
BsmAI	GTCTC	3 cut(s)	536, 1089, 1293
BsmFI	GGGAC	3 cut(s)	225, 507, 732
BsmI	GAATGC	2 cut(s)	748, 1177
Bsp119I	TTCGAA	2 cut(s)	358, 1181
Bsp1286I	GDGCHC	1 cut(s)	835
Bsp143I	GATC	9 cut(s)	107, 379, 436, 547, 845, 921, 989, 1204, 1327
BspACI	CCGC	8 cut(s)	22, 25, 57, 69, 153, 204, 264, 597
BspCNI	CTCAG	3 cut(s)	281, 301, 1303
BspFNI	CGCG	2 cut(s)	153, 1056
BspLI	GGNNCC	2 cut(s)	202, 549
BspPI	GGATC	6 cut(s)	387, 542, 555, 853, 929, 997
BspQI	GCTCTTC	1 cut(s)	86
BspT104I	TTCGAA	2 cut(s)	358, 1181
BspT107I	GGYRCC	1 cut(s)	200
BsrI	ACTGG	4 cut(s)	633, 1039, 1133, 1167
BssECI	CCNNGG	1 cut(s)	577
BssMI	GATC	9 cut(s)	107, 379, 436, 547, 845, 921, 989, 1204, 1327
BssNI	GRCGYC	1 cut(s)	201
BssSI	CACGAG	1 cut(s)	533
Bst2BI	CACGAG	1 cut(s)	533
Bst2UI	CCWGG	3 cut(s)	298, 579, 1002
Bst4CI	ACNGT	4 cut(s)	468, 742, 1064, 1220
Bst6I	CTCTTC	5 cut(s)	86, 355, 483, 761, 1178
BstACI	GRCGYC	1 cut(s)	201
BstAPI	GCANNNNNTGC	1 cut(s)	1051
BstBI	TTCGAA	2 cut(s)	358, 1181
BstC8I	GCNNGC	1 cut(s)	1250
BstDEI	CTNAG	6 cut(s)	268, 309, 430, 1149, 1263, 1290
BstEII	GGTNACC	1 cut(s)	292
BstF5I	GGATG	2 cut(s)	27, 1115
BstFNI	CGCG	2 cut(s)	153, 1056
BstH2I	RGCGCY	3 cut(s)	204, 284, 398
BstHHI	GCGC	6 cut(s)	153, 203, 283, 397, 1056, 1248
BstKTI	GATC	9 cut(s)	110, 382, 439, 550, 848, 924, 992, 1207, 1330
BstMAI	GTCTC	3 cut(s)	536, 1089, 1293
BstMBI	GATC	9 cut(s)	107, 379, 436, 547, 845, 921, 989, 1204, 1327
BstMWI	GCNNNNNNNGC	2 cut(s)	234, 1051
BstNI	CCWGG	3 cut(s)	298, 579, 1002
BstNSI	RCATGY	2 cut(s)	965, 1381
BstPI	GGTNACC	1 cut(s)	292
BstSCI	CCNGG	3 cut(s)	296, 577, 1000
BstSLI	GKGCMC	1 cut(s)	835
BstUI	CGCG	2 cut(s)	153, 1056
BstV1I	GCAGC	4 cut(s)	240, 290, 619, 1038
BstV2I	GAAGAC	2 cut(s)	144, 440
BstX2I	RGATCY	2 cut(s)	547, 845
BstYI	RGATCY	2 cut(s)	547, 845
BsuI	GTATCC	1 cut(s)	716
BtsCI	GGATG	2 cut(s)	27, 1115
BtsIMutI	CAGTG	4 cut(s)	183, 1060, 1174, 1225
Cac8I	GCNNGC	1 cut(s)	1250
CaiI	CAGNNNCTG	1 cut(s)	303
CfoI	GCGC	6 cut(s)	153, 203, 283, 397, 1056, 1248
CseI	GACGC	1 cut(s)	220
CsiI	ACCWGGT	1 cut(s)	296
Csp6I	GTAC	3 cut(s)	677, 976, 1058
CviAII	CATG	6 cut(s)	376, 800, 962, 996, 1011, 1378
CviQI	GTAC	3 cut(s)	677, 976, 1058
DdeI	CTNAG	6 cut(s)	268, 309, 430, 1149, 1263, 1290
DinI	GGCGCC	1 cut(s)	202
DpnI	GATC	9 cut(s)	109, 381, 438, 549, 847, 923, 991, 1206, 1329
DpnII	GATC	9 cut(s)	107, 379, 436, 547, 845, 921, 989, 1204, 1327
DraIII	CACNNNGTG	1 cut(s)	300
Eam1104I	CTCTTC	5 cut(s)	86, 355, 483, 761, 1178
EarI	CTCTTC	5 cut(s)	86, 355, 483, 761, 1178
EciI	GGCGGA	2 cut(s)	11, 46
Eco47III	AGCGCT	1 cut(s)	396
Eco57I	CTGAAG	1 cut(s)	765
Eco91I	GGTNACC	1 cut(s)	292
EcoO65I	GGTNACC	1 cut(s)	292
EcoRII	CCWGG	3 cut(s)	296, 577, 1000
EgeI	GGCGCC	1 cut(s)	202
EheI	GGCGCC	1 cut(s)	202
FaeI	CATG	6 cut(s)	379, 803, 965, 999, 1014, 1381
FaqI	GGGAC	3 cut(s)	225, 507, 732
FatI	CATG	6 cut(s)	375, 799, 961, 995, 1010, 1377
FblI	GTMKAC	1 cut(s)	900
Fnu4HI	GCNGC	9 cut(s)	25, 70, 154, 204, 229, 264, 304, 633, 1052
FokI	GGATG	2 cut(s)	14, 1122
Fsp4HI	GCNGC	9 cut(s)	25, 70, 154, 204, 229, 264, 304, 633, 1052
FspBI	CTAG	2 cut(s)	1340, 1349
GlaI	GCGC	6 cut(s)	152, 202, 282, 396, 1055, 1247
GluI	GCNGC	9 cut(s)	25, 70, 154, 204, 229, 264, 304, 633, 1052
HaeII	RGCGCY	3 cut(s)	204, 284, 398
HapII	CCGG	1 cut(s)	66
HgaI	GACGC	1 cut(s)	220
HhaI	GCGC	6 cut(s)	153, 203, 283, 397, 1056, 1248
Hin1I	GRCGYC	1 cut(s)	201
Hin1II	CATG	6 cut(s)	379, 803, 965, 999, 1014, 1381
Hin6I	GCGC	6 cut(s)	151, 201, 281, 395, 1054, 1246
HinP1I	GCGC	6 cut(s)	151, 201, 281, 395, 1054, 1246
HinfI	GANTC	6 cut(s)	7, 62, 188, 313, 419, 606
HpaII	CCGG	1 cut(s)	66
HphI	GGTGA	3 cut(s)	81, 286, 916
Hpy166II	GTNNAC	6 cut(s)	168, 183, 196, 679, 901, 1060
Hpy188I	TCNGA	5 cut(s)	373, 661, 784, 916, 1094
Hpy188III	TCNNGA	5 cut(s)	105, 134, 274, 331, 1335
Hpy8I	GTNNAC	6 cut(s)	168, 183, 196, 679, 901, 1060
Hpy99I	CGWCG	2 cut(s)	236, 280
HpyAV	CCTTC	3 cut(s)	23, 816, 1380
HpyCH4III	ACNGT	4 cut(s)	468, 742, 1064, 1220
HpyCH4V	TGCA	9 cut(s)	737, 748, 817, 965, 1067, 1252, 1300, 1315, 1381
HpyF10VI	GCNNNNNNNGC	2 cut(s)	234, 1051
HpyF3I	CTNAG	6 cut(s)	268, 309, 430, 1149, 1263, 1290
Hsp92I	GRCGYC	1 cut(s)	201
Hsp92II	CATG	6 cut(s)	379, 803, 965, 999, 1014, 1381
HspAI	GCGC	6 cut(s)	151, 201, 281, 395, 1054, 1246
KasI	GGCGCC	1 cut(s)	200
Kzo9I	GATC	9 cut(s)	107, 379, 436, 547, 845, 921, 989, 1204, 1327
LguI	GCTCTTC	1 cut(s)	86
LmnI	GCTCC	2 cut(s)	776, 1366
Lsp1109I	GCAGC	4 cut(s)	240, 290, 619, 1038
LweI	GCATC	3 cut(s)	36, 377, 1261
MabI	ACCWGGT	1 cut(s)	296
MaeI	CTAG	2 cut(s)	1340, 1349
MaeIII	GTNAC	3 cut(s)	271, 292, 647
MalI	GATC	9 cut(s)	109, 381, 438, 549, 847, 923, 991, 1206, 1329
MboI	GATC	9 cut(s)	107, 379, 436, 547, 845, 921, 989, 1204, 1327
MflI	RGATCY	2 cut(s)	547, 845
MhlI	GDGCHC	1 cut(s)	835
MluCI	AATT	5 cut(s)	441, 1023, 1240, 1352, 1402
Mly113I	GGCGCC	1 cut(s)	201
MlyI	GAGTC	2 cut(s)	182, 413
MmeI	TCCRAC	1 cut(s)	1072
MroXI	GAANNNNTTC	1 cut(s)	467
MseI	TTAA	5 cut(s)	285, 347, 399, 644, 869
MslI	CAYNNNNRTG	3 cut(s)	501, 929, 1167
MspA1I	CMGCKG	1 cut(s)	1051
MspI	CCGG	1 cut(s)	66
MspR9I	CCNGG	3 cut(s)	298, 579, 1002
Mva1269I	GAATGC	2 cut(s)	748, 1177
MvaI	CCWGG	3 cut(s)	298, 579, 1002
MvnI	CGCG	2 cut(s)	153, 1056
MwoI	GCNNNNNNNGC	2 cut(s)	234, 1051
NarI	GGCGCC	1 cut(s)	201
NdeII	GATC	9 cut(s)	107, 379, 436, 547, 845, 921, 989, 1204, 1327
NlaIII	CATG	6 cut(s)	379, 803, 965, 999, 1014, 1381
NlaIV	GGNNCC	2 cut(s)	202, 549
NmuCI	GTSAC	2 cut(s)	271, 292
NspI	RCATGY	2 cut(s)	965, 1381
NspV	TTCGAA	2 cut(s)	358, 1181
OliI	CACNNNNGTG	1 cut(s)	929
PciSI	GCTCTTC	1 cut(s)	86
PctI	GAATGC	2 cut(s)	748, 1177
PdmI	GAANNNNTTC	1 cut(s)	467
PfeI	GAWTC	4 cut(s)	7, 62, 313, 606
PflMI	CCANNNNNTGG	1 cut(s)	1034
PkrI	GCNGC	9 cut(s)	26, 71, 155, 205, 230, 265, 305, 634, 1053
PleI	GAGTC	2 cut(s)	182, 413
PluTI	GGCGCC	1 cut(s)	204
PpsI	GAGTC	2 cut(s)	182, 413
Psp6I	CCWGG	3 cut(s)	296, 577, 1000
PspEI	GGTNACC	1 cut(s)	292
PspGI	CCWGG	3 cut(s)	296, 577, 1000
PspN4I	GGNNCC	2 cut(s)	202, 549
PstNI	CAGNNNCTG	1 cut(s)	303
PsuI	RGATCY	2 cut(s)	547, 845
PvuII	CAGCTG	1 cut(s)	1051
RsaI	GTAC	3 cut(s)	678, 977, 1059
RsaNI	GTAC	3 cut(s)	677, 976, 1058
RseI	CAYNNNNRTG	3 cut(s)	501, 929, 1167
SapI	GCTCTTC	1 cut(s)	86
SaqAI	TTAA	5 cut(s)	285, 347, 399, 644, 869
SatI	GCNGC	9 cut(s)	25, 70, 154, 204, 229, 264, 304, 633, 1052
Sau3AI	GATC	9 cut(s)	107, 379, 436, 547, 845, 921, 989, 1204, 1327
SchI	GAGTC	2 cut(s)	182, 413
ScrFI	CCNGG	3 cut(s)	298, 579, 1002
SduI	GDGCHC	1 cut(s)	835
SexAI	ACCWGGT	1 cut(s)	296
SfaNI	GCATC	3 cut(s)	36, 377, 1261
SfoI	GGCGCC	1 cut(s)	202
SfuI	TTCGAA	2 cut(s)	358, 1181
SmiMI	CAYNNNNRTG	3 cut(s)	501, 929, 1167
Sse9I	AATT	5 cut(s)	441, 1023, 1240, 1352, 1402
SsiI	CCGC	8 cut(s)	22, 25, 57, 69, 153, 204, 264, 597
SspDI	GGCGCC	1 cut(s)	200
SspMI	CTAG	2 cut(s)	1340, 1349
StyD4I	CCNGG	3 cut(s)	296, 577, 1000
TaaI	ACNGT	4 cut(s)	468, 742, 1064, 1220
TaqI	TCGA	5 cut(s)	358, 422, 439, 1039, 1181
TasI	AATT	5 cut(s)	441, 1023, 1240, 1352, 1402
TatI	WGTACW	1 cut(s)	975
TauI	GCSGC	5 cut(s)	27, 72, 156, 206, 266
TfiI	GAWTC	4 cut(s)	7, 62, 313, 606
Tru1I	TTAA	5 cut(s)	285, 347, 399, 644, 869
Tru9I	TTAA	5 cut(s)	285, 347, 399, 644, 869
TscAI	CASTG	4 cut(s)	190, 1067, 1174, 1225
TseFI	GTSAC	2 cut(s)	271, 292
TseI	GCWGC	4 cut(s)	228, 303, 632, 1051
Tsp45I	GTSAC	2 cut(s)	271, 292
TspDTI	ATGAA	5 cut(s)	73, 440, 639, 1247, 1362
TspGWI	ACGGA	7 cut(s)	26, 168, 181, 736, 749, 865, 1334
TspRI	CASTG	4 cut(s)	190, 1067, 1174, 1225
Van91I	CCANNNNNTGG	1 cut(s)	1034
XapI	RAATTY	3 cut(s)	441, 1240, 1402
XceI	RCATGY	2 cut(s)	965, 1381
XmiI	GTMKAC	1 cut(s)	900
XmnI	GAANNNNTTC	1 cut(s)	467
XspI	CTAG	2 cut(s)	1340, 1349

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.