pycom10g22650

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0001843
Physical Location & Seq
Forward (+)
24977551 .. 24980563
3013 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1128 bp
ATGGCTGGTGGTACGAAACTATTGGCTTTGGTGATTGCTCTATCTTGGGTATCGAACATGCAGCAATTCGTTCAGGGAAAGCCACAAGCTGTCCTGGGAAAGCCACAAGTGCCTTGCTTTTTCATATTTGGAGACTCAGTAGCTGATCCTGGCAACAACAACAAGCTTCCCACATTGGCTAAAGTCAACTACAAGCCGTATGGTATTGACTTCCCCGCTGGCCCAACAGGAAGGTTCTGCAATGGCCGAAACATCGTTGATATTCTTGCTGAACTTCTGGGATTTGAAAACTACATTCCAGCATTTGCTGATGCTAATGGCACGGACATACTCCAAGGAGTGAACTATGCATCTGGTGCAGCAGGAATTAGAAAAGAAAGTGGAAAGCAGCTGGGTGCACGTATTAGCATGGGGGAACAGTTGAAGAATCACAGAACTACAGTGTCGCGCATTATCGACATATTTGGAAAGAAAAGCTTAGCCAAAAATCAATTAAACCAGTGCTTATATTCAGTTGGAATGGGAAGCAATGACTACATCAACAATTACTTCATGCCTCAGTATTACACCACCAGTCAAAAATATACCCTTGAGGAATATGCTGATGTTCTTATTAAACAGTACACTCGCCAAATACTGAAGTTGCACCAGTATGGAGCAAGGAAGGTGGCCTTGGTTGGACTGGGACTAATAGGCTGCACTCCAGATGCAATTAAGACCTATGGCACAAATGGGTCTGCATGTGTGGATATAATGAACAATGCATCCCAAATTTTCAACGGAAAACTTGTTTCTCTTGTGGACGAGCTCAACACCAATCTCACTGACTCACAATTTATCTATATCAACAGTTATGGAATGGGCTCAGGGGACGCTACATTAGCTGGGTTCAAGGTTTTGAATGCTGGGTGCTGTGAAGCAGATGAAGTTGGTCAGTGCGTTCCCGATCTAACTCCATGCCAGAATAGGAGTGAATATGTGTTTTGGGACTCATTCCATCCTACTGAGGCCGGCAATTTGATCAGTGCAAGCAGAACATACACAGCTTCTGAACCTTCAGACACATATCCTATGGATATGAGTCAATTAGCTGAGCTGCAAATTAATCCTCAAGTCAACGAGATCTAG

Protein Analysis

376

Amino Acids

40.85

Weight (kDa)

5.51

Isoelectric Point (pI)

21.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 41 - 344 1.5e-31 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 448
AciI CCGC 1 cut(s) 216
AclWI GGATC 1 cut(s) 140
AcoI YGGCCR 1 cut(s) 244
AcsI RAATTY 1 cut(s) 771
AcuI CTGAAG 2 cut(s) 659, 1041
AfaI GTAC 2 cut(s) 13, 623
AgsI TTSAA 5 cut(s) 287, 424, 778, 892, 901
AjnI CCWGG 2 cut(s) 93, 148
AjuI GAANNNNNNNTTGG 2 cut(s) 656, 688
AloI GAACNNNNNNTCC 2 cut(s) 749, 781
Alw21I GWGCWC 2 cut(s) 400, 810
Alw26I GTCTC 1 cut(s) 126
Alw44I GTGCAC 1 cut(s) 396
AlwI GGATC 1 cut(s) 140
AlwNI CAGNNNCTG 2 cut(s) 143, 1049
AoxI GGCC 4 cut(s) 220, 244, 669, 1008
ApaLI GTGCAC 1 cut(s) 396
ApeKI GCWGC 5 cut(s) 61, 359, 388, 696, 1096
ApoI RAATTY 1 cut(s) 771
AseI ATTAAT 1 cut(s) 1104
AspLEI GCGC 1 cut(s) 450
AspS9I GGNCC 1 cut(s) 221
AsuHPI GGTGA 1 cut(s) 43
BaeGI GKGCMC 1 cut(s) 400
BanII GRGCYC 2 cut(s) 810, 866
Bbv12I GWGCWC 2 cut(s) 400, 810
BbvI GCAGC 5 cut(s) 73, 371, 400, 683, 1083
BccI CCATC 1 cut(s) 1005
BceAI ACGGC 1 cut(s) 181
BciT130I CCWGG 2 cut(s) 95, 150
BclI TGATCA 1 cut(s) 1020
BcoDI GTCTC 1 cut(s) 126
BfaI CTAG 1 cut(s) 1126
BfmI CTRYAG 1 cut(s) 438
BglII AGATCT 1 cut(s) 1122
BisI GCNGC 5 cut(s) 62, 360, 389, 697, 1097
BlpI GCTNAGC 2 cut(s) 478, 1092
BlsI GCNGC 5 cut(s) 63, 361, 390, 698, 1098
Bme1390I CCNGG 2 cut(s) 95, 150
BmgT120I GGNCC 1 cut(s) 221
BmrFI CCNGG 2 cut(s) 95, 150
BmrI ACTGGG 1 cut(s) 692
BmsI GCATC 4 cut(s) 301, 359, 697, 773
BmuI ACTGGG 1 cut(s) 692
BpmI CTGGAG 1 cut(s) 687
Bpu10I CCTNAGC 1 cut(s) 865
Bpu1102I GCTNAGC 2 cut(s) 478, 1092
BpuEI CTTGAG 2 cut(s) 611, 1095
BsaAI YACGTR 1 cut(s) 401
BsaJI CCNNGG 3 cut(s) 94, 334, 672
BsaXI ACNNNNNCTCC 2 cut(s) 123, 153
Bse118I RCCGGY 1 cut(s) 1010
Bse1I ACTGG 4 cut(s) 499, 573, 649, 687
Bse3DI GCAATG 2 cut(s) 247, 535
BseBI CCWGG 2 cut(s) 95, 150
BseDI CCNNGG 3 cut(s) 94, 334, 672
BseGI GGATG 2 cut(s) 764, 997
BseMI GCAATG 2 cut(s) 247, 535
BseMII CTCAG 5 cut(s) 150, 572, 879, 996, 1083
BseNI ACTGG 4 cut(s) 499, 573, 649, 687
BseSI GKGCMC 1 cut(s) 400
BseXI GCAGC 5 cut(s) 73, 371, 400, 683, 1083
BseYI CCCAGC 3 cut(s) 391, 884, 905
BsgI GTGCAG 2 cut(s) 378, 682
Bsh1236I CGCG 1 cut(s) 448
BshFI GGCC 4 cut(s) 222, 246, 671, 1010
BsiHKAI GWGCWC 2 cut(s) 400, 810
BsiSI CCGG 1 cut(s) 1011
BslFI GGGAC 3 cut(s) 699, 884, 1001
BsmAI GTCTC 1 cut(s) 126
BsmFI GGGAC 3 cut(s) 699, 884, 1001
BsmI GAATGC 1 cut(s) 907
BsnI GGCC 4 cut(s) 222, 246, 671, 1010
Bsp1286I GDGCHC 3 cut(s) 400, 810, 866
Bsp143I GATC 4 cut(s) 145, 946, 1020, 1122
Bsp1720I GCTNAGC 2 cut(s) 478, 1092
BspACI CCGC 1 cut(s) 216
BspANI GGCC 4 cut(s) 222, 246, 671, 1010
BspCNI CTCAG 5 cut(s) 149, 571, 878, 997, 1084
BspFNI CGCG 1 cut(s) 448
BspPI GGATC 1 cut(s) 140
BsrDI GCAATG 2 cut(s) 247, 535
BsrFI RCCGGY 1 cut(s) 1010
BsrI ACTGG 4 cut(s) 499, 573, 649, 687
BssAI RCCGGY 1 cut(s) 1010
BssECI CCNNGG 3 cut(s) 94, 334, 672
BssMI GATC 4 cut(s) 145, 946, 1020, 1122
BssT1I CCWWGG 2 cut(s) 334, 672
Bst2UI CCWGG 2 cut(s) 95, 150
Bst4CI ACNGT 4 cut(s) 420, 442, 621, 851
BstAPI GCANNNNNTGC 1 cut(s) 356
BstBAI YACGTR 1 cut(s) 401
BstC8I GCNNGC 3 cut(s) 220, 1012, 1030
BstDEI CTNAG 6 cut(s) 136, 478, 558, 865, 1005, 1092
BstF5I GGATG 2 cut(s) 764, 997
BstFNI CGCG 1 cut(s) 448
BstHHI GCGC 1 cut(s) 450
BstKTI GATC 4 cut(s) 148, 949, 1023, 1125
BstMAI GTCTC 1 cut(s) 126
BstMBI GATC 4 cut(s) 145, 946, 1020, 1122
BstMWI GCNNNNNNNGC 3 cut(s) 109, 356, 881
BstNI CCWGG 2 cut(s) 95, 150
BstNSI RCATGY 2 cut(s) 61, 744
BstSCI CCNGG 2 cut(s) 93, 148
BstSFI CTRYAG 1 cut(s) 438
BstSLI GKGCMC 1 cut(s) 400
BstUI CGCG 1 cut(s) 448
BstV1I GCAGC 5 cut(s) 73, 371, 400, 683, 1083
BstX2I RGATCY 1 cut(s) 1122
BstYI RGATCY 1 cut(s) 1122
BsuRI GGCC 4 cut(s) 222, 246, 671, 1010
BtsCI GGATG 2 cut(s) 764, 997
BtsIMutI CAGTG 5 cut(s) 447, 506, 822, 941, 1030
Cac8I GCNNGC 3 cut(s) 220, 1012, 1030
CaiI CAGNNNCTG 2 cut(s) 143, 1049
CfoI GCGC 1 cut(s) 450
Cfr10I RCCGGY 1 cut(s) 1010
Cfr13I GGNCC 1 cut(s) 221
CseI GACGC 1 cut(s) 881
Csp6I GTAC 2 cut(s) 12, 622
CspCI CAANNNNNGTGG 2 cut(s) 648, 683
CviAII CATG 5 cut(s) 58, 409, 553, 741, 957
CviQI GTAC 2 cut(s) 12, 622
DdeI CTNAG 6 cut(s) 136, 478, 558, 865, 1005, 1092
DpnI GATC 4 cut(s) 147, 948, 1022, 1124
DpnII GATC 4 cut(s) 145, 946, 1020, 1122
EaeI YGGCCR 1 cut(s) 244
Ecl136II GAGCTC 1 cut(s) 808
Eco130I CCWWGG 2 cut(s) 334, 672
Eco24I GRGCYC 2 cut(s) 810, 866
Eco53kI GAGCTC 1 cut(s) 808
Eco57I CTGAAG 2 cut(s) 659, 1041
EcoICRI GAGCTC 1 cut(s) 808
EcoRII CCWGG 2 cut(s) 93, 148
EcoT14I CCWWGG 2 cut(s) 334, 672
EcoT22I ATGCAT 2 cut(s) 352, 766
EcoT38I GRGCYC 2 cut(s) 810, 866
ErhI CCWWGG 2 cut(s) 334, 672
FaeI CATG 5 cut(s) 61, 412, 556, 744, 960
FalI AAGNNNNNCTT 4 cut(s) 461, 493, 656, 688
FaqI GGGAC 3 cut(s) 699, 884, 1001
FatI CATG 5 cut(s) 57, 408, 552, 740, 956
FauI CCCGC 1 cut(s) 223
FbaI TGATCA 1 cut(s) 1020
Fnu4HI GCNGC 5 cut(s) 62, 360, 389, 697, 1097
FokI GGATG 2 cut(s) 751, 984
FriOI GRGCYC 2 cut(s) 810, 866
Fsp4HI GCNGC 5 cut(s) 62, 360, 389, 697, 1097
FspBI CTAG 1 cut(s) 1126
GlaI GCGC 1 cut(s) 449
GluI GCNGC 5 cut(s) 62, 360, 389, 697, 1097
GsaI CCCAGC 3 cut(s) 395, 888, 909
GsuI CTGGAG 1 cut(s) 687
HaeIII GGCC 4 cut(s) 222, 246, 671, 1010
HapII CCGG 1 cut(s) 1011
HgaI GACGC 1 cut(s) 881
HhaI GCGC 1 cut(s) 450
Hin1II CATG 5 cut(s) 61, 412, 556, 744, 960
Hin6I GCGC 1 cut(s) 448
HinP1I GCGC 1 cut(s) 448
HincII GTYRAC 2 cut(s) 187, 1117
HindII GTYRAC 2 cut(s) 187, 1117
HindIII AAGCTT 2 cut(s) 164, 475
HinfI GANTC 5 cut(s) 134, 427, 827, 989, 1081
HpaII CCGG 1 cut(s) 1011
HphI GGTGA 1 cut(s) 43
Hpy166II GTNNAC 6 cut(s) 187, 343, 398, 624, 802, 1117
Hpy188I TCNGA 2 cut(s) 1051, 1060
Hpy188III TCNNGA 2 cut(s) 704, 944
Hpy8I GTNNAC 6 cut(s) 187, 343, 398, 624, 802, 1117
HpyAV CCTTC 3 cut(s) 225, 658, 1065
HpyCH4III ACNGT 4 cut(s) 420, 442, 621, 851
HpyCH4IV ACGT 1 cut(s) 400
HpyF10VI GCNNNNNNNGC 3 cut(s) 109, 356, 881
HpyF3I CTNAG 6 cut(s) 136, 478, 558, 865, 1005, 1092
HpySE526I ACGT 1 cut(s) 400
Hsp92II CATG 5 cut(s) 61, 412, 556, 744, 960
HspAI GCGC 1 cut(s) 448
KroI GCCGGC 1 cut(s) 1010
KroNI GCCGGC 1 cut(s) 1012
Ksp22I TGATCA 1 cut(s) 1020
Kzo9I GATC 4 cut(s) 145, 946, 1020, 1122
LmnI GCTCC 1 cut(s) 656
Lsp1109I GCAGC 5 cut(s) 73, 371, 400, 683, 1083
LweI GCATC 4 cut(s) 301, 359, 697, 773
MaeI CTAG 1 cut(s) 1126
MaeII ACGT 1 cut(s) 400
MalI GATC 4 cut(s) 147, 948, 1022, 1124
MboI GATC 4 cut(s) 145, 946, 1020, 1122
MboII GAAGA 1 cut(s) 436
MflI RGATCY 1 cut(s) 1122
MhlI GDGCHC 3 cut(s) 400, 810, 866
MlyI GAGTC 4 cut(s) 128, 821, 983, 1090
MmeI TCCRAC 2 cut(s) 496, 658
MnlI CCTC 4 cut(s) 567, 586, 1000, 1119
Mph1103I ATGCAT 2 cut(s) 352, 766
MroNI GCCGGC 1 cut(s) 1010
MseI TTAA 4 cut(s) 494, 615, 714, 1104
MslI CAYNNNNRTG 1 cut(s) 651
MspA1I CMGCKG 2 cut(s) 218, 391
MspI CCGG 1 cut(s) 1011
MspR9I CCNGG 2 cut(s) 95, 150
Mva1269I GAATGC 1 cut(s) 907
MvaI CCWGG 2 cut(s) 95, 150
MvnI CGCG 1 cut(s) 448
MwoI GCNNNNNNNGC 3 cut(s) 109, 356, 881
NaeI GCCGGC 1 cut(s) 1012
NdeII GATC 4 cut(s) 145, 946, 1020, 1122
NgoMIV GCCGGC 1 cut(s) 1010
NlaIII CATG 5 cut(s) 61, 412, 556, 744, 960
NsiI ATGCAT 2 cut(s) 352, 766
NspI RCATGY 2 cut(s) 61, 744
PctI GAATGC 1 cut(s) 907
PdiI GCCGGC 1 cut(s) 1012
PfeI GAWTC 1 cut(s) 427
PkrI GCNGC 5 cut(s) 63, 361, 390, 698, 1098
PleI GAGTC 4 cut(s) 128, 821, 983, 1089
PpsI GAGTC 4 cut(s) 128, 821, 983, 1089
Ppu21I YACGTR 1 cut(s) 401
PshBI ATTAAT 1 cut(s) 1104
Psp124BI GAGCTC 1 cut(s) 810
Psp6I CCWGG 2 cut(s) 93, 148
PspFI CCCAGC 3 cut(s) 391, 884, 905
PspGI CCWGG 2 cut(s) 93, 148
PspPI GGNCC 1 cut(s) 221
PstNI CAGNNNCTG 2 cut(s) 143, 1049
PsuI RGATCY 1 cut(s) 1122
PvuII CAGCTG 1 cut(s) 391
RsaI GTAC 2 cut(s) 13, 623
RsaNI GTAC 2 cut(s) 12, 622
RseI CAYNNNNRTG 1 cut(s) 651
SacI GAGCTC 1 cut(s) 810
SaqAI TTAA 4 cut(s) 494, 615, 714, 1104
SatI GCNGC 5 cut(s) 62, 360, 389, 697, 1097
Sau3AI GATC 4 cut(s) 145, 946, 1020, 1122
Sau96I GGNCC 1 cut(s) 221
SchI GAGTC 4 cut(s) 128, 821, 983, 1090
ScrFI CCNGG 2 cut(s) 95, 150
SduI GDGCHC 3 cut(s) 400, 810, 866
SfaNI GCATC 4 cut(s) 301, 359, 697, 773
SfcI CTRYAG 1 cut(s) 438
SmiMI CAYNNNNRTG 1 cut(s) 651
SmlI CTYRAG 2 cut(s) 590, 1110
SmoI CTYRAG 2 cut(s) 590, 1110
SsiI CCGC 1 cut(s) 216
SspMI CTAG 1 cut(s) 1126
SstI GAGCTC 1 cut(s) 810
StyD4I CCNGG 2 cut(s) 93, 148
StyI CCWWGG 2 cut(s) 334, 672
TaaI ACNGT 4 cut(s) 420, 442, 621, 851
TaiI ACGT 1 cut(s) 403
TaqI TCGA 2 cut(s) 53, 456
TatI WGTACW 1 cut(s) 621
TfiI GAWTC 1 cut(s) 427
Tru1I TTAA 4 cut(s) 494, 615, 714, 1104
Tru9I TTAA 4 cut(s) 494, 615, 714, 1104
TscAI CASTG 5 cut(s) 447, 506, 829, 941, 1030
TseI GCWGC 5 cut(s) 61, 359, 388, 696, 1096
TspDTI ATGAA 4 cut(s) 112, 541, 770, 939
TspGWI ACGGA 2 cut(s) 338, 795
TspRI CASTG 5 cut(s) 447, 506, 829, 941, 1030
VneI GTGCAC 1 cut(s) 396
VspI ATTAAT 1 cut(s) 1104
XapI RAATTY 1 cut(s) 771
XceI RCATGY 2 cut(s) 61, 744
XspI CTAG 1 cut(s) 1126
Zsp2I ATGCAT 2 cut(s) 352, 766
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.