pycom12g00050

Belongs to the GDA1 CD39 NTPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001988
Physical Location & Seq
Forward (+)
108881 .. 111545
2665 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1311 bp
ATGCTCAAGCGTCCCGGCAGAGGAATTGGAATGGGGAATCCCCAGTACGACTCCCTCTCCGACAGAATATACAGATACAGGAGCGTCATCCTCGTCATCTCAATCCCTCTCCTCCTCCTTACCATGGTCCTCTACGTCATGCCTCCGGGGGGTAATTTGAGCAACCAGCTCCCCCGCAAGCTGCCTTCCAAGACCTCCTTCGCCGTGATCGTTCGGAGGAACGCCGGCAGCTCCGGCAGCCGCGTCCACGTCTTCTGCTTCGACCACAACCTCCAGCTCCTTCCCATCGGCAAAGATCTCGAGCTCTTTCTTCAGGTCAAGCCTGGCCTGAGCGCTTATGCCAAGGACCCTCGCTCTGCTGCCGAGTCCCTCACCAGTCTTCTCGATAAGGCTGAGAGCGCAGTTCCCAAGGAGCTACGTTCCTTCACTCCCGTCCGCGTTGGGGCTACGGCTGGCCTGAGGGCTTTGAAGGGCGATGCTTCTGACCGCATTCTCCAGGCCGTTAGGGATCTGCTCAAACAAAGAAGCAGCCTCAGCTCCCGCCCTGATGCCGTTACTGTCATTGATGGAACTCAAGAGGGTTCTTTCCAGTGGGTGACAATAAACTATCTACTAGGAAATTTGGGGAAGAAATATTCAGATACTGTAGGGGTGGTTGACCTTGGGGGTGGATCTGTTCAGATGGCTTATGCTATCTCAGAGGCGGATGCTGCAAAAGCACCCAAGTTATCAGATGGAGAGGATGTATATGTAAGGGAAATGAATCTTAAAGGAGCTAAATATAACCTTTATGTTCACAGTTACTTGCATTACGGGTTACTAGCAGCTCGAGCAGAGATTTTAAAGGTTTCTGAAGATTCTGCCAACCCCTGCATCTTAGCTGGATATTACGGTTCTTACACATATGGAGGACAATCTTATAAAGCATCAGCTTCCTCATCTGGATCAAGTGTTGAGGAGTGCAGGGGGGTAGTTACAAAGGCACTCAAAGTAAACAAAGCGACATGTTCACACATGAAGTGCACATTTGGTGGGGTATGGAACGGTGGAGGAGGGGATGGACAAAGGAACCTATTTGTGGCTTCATTTTTCTTTGACAGAGCTGCTGAGGCTGGTTTTGTTAACCCAGACGAACCTGTTGCCAAAGTTCACCCTGGTAACTTTGAGGAGGCAGCTAAGCGTGCTTGTGAAACTAAACTTGAGGATGCTAAATCAACGTATCCACAAGTTGAGGAGGGAAACCTGCCATTCTTGTGCATGGATCTAGTCTACCAATCTACATTGCTTGTGGATGGATTTGGTGGGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 map00230 map00240
Pfam Domains
Protein Families
GDA1_CD39

Protein Analysis

437

Amino Acids

47.02

Weight (kDa)

8.76

Isoelectric Point (pI)

41.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GDA1_CD39 PF01150 63 - 434 1.3e-94 GDA1/CD39 (nucleoside phosphatase) family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 921
Acc36I ACCTGC 1 cut(s) 1251
AccI GTMKAC 1 cut(s) 1269
AccII CGCG 2 cut(s) 243, 438
AciI CCGC 6 cut(s) 175, 241, 436, 487, 541, 704
AclWI GGATC 4 cut(s) 516, 679, 952, 1269
AcsI RAATTY 1 cut(s) 619
AcuI CTGAAG 2 cut(s) 296, 873
AfaI GTAC 1 cut(s) 47
AfeI AGCGCT 1 cut(s) 334
AfiI CCNNNNNNNGG 5 cut(s) 20, 124, 149, 442, 1078
AflIII ACRYGT 1 cut(s) 1004
AgsI TTSAA 1 cut(s) 469
AjiI CACGTC 1 cut(s) 250
AjnI CCWGG 3 cut(s) 322, 495, 1153
AjuI GAANNNNNNNTTGG 2 cut(s) 182, 214
Alw21I GWGCWC 2 cut(s) 306, 1025
Alw44I GTGCAC 1 cut(s) 1021
AlwI GGATC 4 cut(s) 516, 679, 952, 1269
AlwNI CAGNNNCTG 1 cut(s) 644
Ama87I CYCGRG 2 cut(s) 299, 828
Aor51HI AGCGCT 1 cut(s) 334
AoxI GGCC 3 cut(s) 325, 454, 498
ApaLI GTGCAC 1 cut(s) 1021
ApeKI GCWGC 9 cut(s) 181, 228, 237, 359, 528, 710, 824, 1103, 1172
ApoI RAATTY 1 cut(s) 619
AspLEI GCGC 2 cut(s) 335, 401
AspS9I GGNCC 2 cut(s) 127, 346
AsuC2I CCSGG 2 cut(s) 15, 147
AsuHPI GGTGA 3 cut(s) 364, 607, 1142
AvaI CYCGRG 2 cut(s) 299, 828
AvaII GGWCC 2 cut(s) 127, 346
AxyI CCTNAGG 1 cut(s) 458
BaeGI GKGCMC 1 cut(s) 1025
BanII GRGCYC 1 cut(s) 306
BbsI GAAGAC 2 cut(s) 244, 371
Bbv12I GWGCWC 2 cut(s) 306, 1025
BbvCI CCTCAGC 2 cut(s) 533, 1107
BbvI GCAGC 9 cut(s) 168, 240, 249, 346, 540, 697, 836, 1090, 1184
BccI CCATC 6 cut(s) 293, 560, 676, 728, 1052, 1286
BceAI ACGGC 4 cut(s) 188, 465, 485, 536
BcgI CGANNNNNNTGC 4 cut(s) 280, 314, 1121, 1155
BciT130I CCWGG 3 cut(s) 324, 497, 1155
BciVI GTATCC 1 cut(s) 1230
BcnI CCSGG 2 cut(s) 15, 147
BfaI CTAG 3 cut(s) 614, 821, 1265
BfmI CTRYAG 1 cut(s) 645
BfoI RGCGCY 1 cut(s) 336
BfuAI ACCTGC 1 cut(s) 1251
BfuI GTATCC 1 cut(s) 1230
BglII AGATCT 1 cut(s) 295
BlpI GCTNAGC 1 cut(s) 1176
Bme1390I CCNGG 5 cut(s) 15, 147, 324, 497, 1155
Bme18I GGWCC 2 cut(s) 127, 346
BmeT110I CYCGRG 2 cut(s) 299, 828
BmgBI CACGTC 1 cut(s) 250
BmgT120I GGNCC 2 cut(s) 127, 346
BmiI GGNNCC 2 cut(s) 348, 1070
BmrFI CCNGG 5 cut(s) 15, 147, 324, 497, 1155
BmrI ACTGGG 1 cut(s) 37
BmsI GCATC 6 cut(s) 466, 538, 697, 882, 935, 1195
BmuI ACTGGG 1 cut(s) 37
BpiI GAAGAC 2 cut(s) 244, 371
BpmI CTGGAG 2 cut(s) 257, 479
Bpu10I CCTNAGC 3 cut(s) 329, 533, 1107
Bpu1102I GCTNAGC 1 cut(s) 1176
BpuEI CTTGAG 2 cut(s) 558, 1220
BpuMI CCSGG 2 cut(s) 15, 147
BsaJI CCNNGG 6 cut(s) 123, 146, 342, 408, 661, 1153
BsaXI ACNNNNNCTCC 4 cut(s) 41, 71, 729, 759
Bsc4I CCNNNNNNNGG 5 cut(s) 20, 124, 149, 442, 1078
Bse118I RCCGGY 1 cut(s) 224
Bse1I ACTGG 3 cut(s) 43, 375, 589
Bse21I CCTNAGG 1 cut(s) 458
Bse3DI GCAATG 1 cut(s) 1280
BseBI CCWGG 3 cut(s) 324, 497, 1155
BseDI CCNNGG 6 cut(s) 123, 146, 342, 408, 661, 1153
BseGI GGATG 6 cut(s) 87, 712, 748, 1063, 1210, 1297
BseLI CCNNNNNNNGG 5 cut(s) 20, 124, 149, 442, 1078
BseMI GCAATG 1 cut(s) 1280
BseMII CTCAG 6 cut(s) 320, 384, 449, 547, 711, 1098
BseNI ACTGG 3 cut(s) 43, 375, 589
BseRI GAGGAG 6 cut(s) 101, 104, 971, 1065, 1181, 1247
BseSI GKGCMC 1 cut(s) 1025
BseXI GCAGC 9 cut(s) 168, 240, 249, 346, 540, 697, 836, 1090, 1184
BsgI GTGCAG 1 cut(s) 982
Bsh1236I CGCG 2 cut(s) 243, 438
BshFI GGCC 3 cut(s) 327, 456, 500
BsiHKAI GWGCWC 2 cut(s) 306, 1025
BsiHKCI CYCGRG 2 cut(s) 299, 828
BsiSI CCGG 4 cut(s) 15, 146, 225, 234
BslFI GGGAC 1 cut(s) 352
BslI CCNNNNNNNGG 5 cut(s) 20, 124, 149, 442, 1078
BsmFI GGGAC 1 cut(s) 352
BsmI GAATGC 1 cut(s) 489
BsnI GGCC 3 cut(s) 327, 456, 500
BsoBI CYCGRG 2 cut(s) 299, 828
Bsp1286I GDGCHC 2 cut(s) 306, 1025
Bsp143I GATC 6 cut(s) 207, 295, 508, 671, 944, 1261
Bsp1720I GCTNAGC 1 cut(s) 1176
Bsp19I CCATGG 1 cut(s) 123
BspACI CCGC 6 cut(s) 175, 241, 436, 487, 541, 704
BspANI GGCC 3 cut(s) 327, 456, 500
BspCNI CTCAG 6 cut(s) 321, 385, 450, 546, 710, 1099
BspFNI CGCG 2 cut(s) 243, 438
BspLI GGNNCC 2 cut(s) 348, 1070
BspMI ACCTGC 1 cut(s) 1251
BspPI GGATC 4 cut(s) 516, 679, 952, 1269
BsrDI GCAATG 1 cut(s) 1280
BsrFI RCCGGY 1 cut(s) 224
BsrI ACTGG 3 cut(s) 43, 375, 589
BssAI RCCGGY 1 cut(s) 224
BssECI CCNNGG 6 cut(s) 123, 146, 342, 408, 661, 1153
BssMI GATC 6 cut(s) 207, 295, 508, 671, 944, 1261
BssT1I CCWWGG 4 cut(s) 123, 342, 408, 661
Bst2UI CCWGG 3 cut(s) 324, 497, 1155
Bst4CI ACNGT 5 cut(s) 559, 646, 800, 893, 1046
BstC8I GCNNGC 4 cut(s) 179, 226, 454, 1182
BstDEI CTNAG 8 cut(s) 329, 393, 458, 533, 697, 877, 1107, 1176
BstDSI CCRYGG 1 cut(s) 123
BstF5I GGATG 6 cut(s) 87, 712, 748, 1063, 1210, 1297
BstFNI CGCG 2 cut(s) 243, 438
BstH2I RGCGCY 1 cut(s) 336
BstHHI GCGC 2 cut(s) 335, 401
BstKTI GATC 6 cut(s) 210, 298, 511, 674, 947, 1264
BstMBI GATC 6 cut(s) 207, 295, 508, 671, 944, 1261
BstMWI GCNNNNNNNGC 9 cut(s) 234, 237, 398, 534, 710, 716, 830, 1109, 1181
BstNI CCWGG 3 cut(s) 324, 497, 1155
BstNSI RCATGY 1 cut(s) 1008
BstSCI CCNGG 5 cut(s) 13, 145, 322, 495, 1153
BstSFI CTRYAG 1 cut(s) 645
BstSLI GKGCMC 1 cut(s) 1025
BstUI CGCG 2 cut(s) 243, 438
BstV1I GCAGC 9 cut(s) 168, 240, 249, 346, 540, 697, 836, 1090, 1184
BstV2I GAAGAC 2 cut(s) 244, 371
BstX2I RGATCY 4 cut(s) 295, 508, 671, 1261
BstYI RGATCY 4 cut(s) 295, 508, 671, 1261
Bsu36I CCTNAGG 1 cut(s) 458
BsuI GTATCC 1 cut(s) 1230
BsuRI GGCC 3 cut(s) 327, 456, 500
BtgI CCRYGG 1 cut(s) 123
BtgZI GCGATG 1 cut(s) 489
BtrI CACGTC 1 cut(s) 250
BtsCI GGATG 6 cut(s) 87, 712, 748, 1063, 1210, 1297
BtsIMutI CAGTG 1 cut(s) 596
BveI ACCTGC 1 cut(s) 1251
Cac8I GCNNGC 4 cut(s) 179, 226, 454, 1182
CaiI CAGNNNCTG 1 cut(s) 644
CfoI GCGC 2 cut(s) 335, 401
Cfr10I RCCGGY 1 cut(s) 224
Cfr13I GGNCC 2 cut(s) 127, 346
CseI GACGC 2 cut(s) 73, 232
Csp6I GTAC 1 cut(s) 46
CviAII CATG 5 cut(s) 124, 139, 1005, 1015, 1258
CviQI GTAC 1 cut(s) 46
DdeI CTNAG 8 cut(s) 329, 393, 458, 533, 697, 877, 1107, 1176
DpnI GATC 6 cut(s) 209, 297, 510, 673, 946, 1263
DpnII GATC 6 cut(s) 207, 295, 508, 671, 944, 1261
DraI TTTAAA 1 cut(s) 843
EciI GGCGGA 1 cut(s) 719
Ecl136II GAGCTC 1 cut(s) 304
Eco130I CCWWGG 4 cut(s) 123, 342, 408, 661
Eco24I GRGCYC 1 cut(s) 306
Eco47I GGWCC 2 cut(s) 127, 346
Eco47III AGCGCT 1 cut(s) 334
Eco53kI GAGCTC 1 cut(s) 304
Eco57I CTGAAG 2 cut(s) 296, 873
Eco81I CCTNAGG 1 cut(s) 458
Eco88I CYCGRG 2 cut(s) 299, 828
EcoICRI GAGCTC 1 cut(s) 304
EcoO109I RGGNCCY 1 cut(s) 346
EcoRII CCWGG 3 cut(s) 322, 495, 1153
EcoT14I CCWWGG 4 cut(s) 123, 342, 408, 661
EcoT38I GRGCYC 1 cut(s) 306
ErhI CCWWGG 4 cut(s) 123, 342, 408, 661
FaeI CATG 5 cut(s) 127, 142, 1008, 1018, 1261
FalI AAGNNNNNCTT 2 cut(s) 182, 214
FaqI GGGAC 1 cut(s) 352
FatI CATG 5 cut(s) 123, 138, 1004, 1014, 1257
FauI CCCGC 2 cut(s) 182, 548
FauNDI CATATG 1 cut(s) 904
FblI GTMKAC 1 cut(s) 1269
FokI GGATG 6 cut(s) 74, 719, 755, 1070, 1217, 1304
FriOI GRGCYC 1 cut(s) 306
FspBI CTAG 3 cut(s) 614, 821, 1265
GlaI GCGC 2 cut(s) 334, 400
GsuI CTGGAG 2 cut(s) 257, 479
HaeII RGCGCY 1 cut(s) 336
HaeIII GGCC 3 cut(s) 327, 456, 500
HapII CCGG 4 cut(s) 15, 146, 225, 234
HgaI GACGC 2 cut(s) 73, 232
HhaI GCGC 2 cut(s) 335, 401
Hin1II CATG 5 cut(s) 127, 142, 1008, 1018, 1261
Hin6I GCGC 2 cut(s) 333, 399
HinP1I GCGC 2 cut(s) 333, 399
HincII GTYRAC 2 cut(s) 658, 1123
HindII GTYRAC 2 cut(s) 658, 1123
HinfI GANTC 5 cut(s) 37, 50, 365, 763, 857
HpaI GTTAAC 1 cut(s) 1123
HpaII CCGG 4 cut(s) 15, 146, 225, 234
HphI GGTGA 3 cut(s) 364, 607, 1142
Hpy166II GTNNAC 9 cut(s) 247, 658, 796, 994, 1010, 1023, 1123, 1150, 1270
Hpy188I TCNGA 8 cut(s) 61, 216, 484, 640, 681, 700, 733, 853
Hpy188III TCNNGA 5 cut(s) 299, 383, 575, 942, 1308
Hpy8I GTNNAC 9 cut(s) 247, 658, 796, 994, 1010, 1023, 1123, 1150, 1270
HpyAV CCTTC 5 cut(s) 195, 208, 290, 433, 463
HpyCH4III ACNGT 5 cut(s) 559, 646, 800, 893, 1046
HpyCH4IV ACGT 4 cut(s) 135, 249, 418, 1217
HpyCH4V TGCA 6 cut(s) 713, 808, 873, 963, 1023, 1257
HpyF10VI GCNNNNNNNGC 9 cut(s) 234, 237, 398, 534, 710, 716, 830, 1109, 1181
HpyF3I CTNAG 8 cut(s) 329, 393, 458, 533, 697, 877, 1107, 1176
HpySE526I ACGT 4 cut(s) 135, 249, 418, 1217
Hsp92II CATG 5 cut(s) 127, 142, 1008, 1018, 1261
HspAI GCGC 2 cut(s) 333, 399
KroI GCCGGC 1 cut(s) 224
KroNI GCCGGC 1 cut(s) 226
KspAI GTTAAC 1 cut(s) 1123
Kzo9I GATC 6 cut(s) 207, 295, 508, 671, 944, 1261
LmnI GCTCC 7 cut(s) 81, 174, 236, 282, 412, 542, 773
Lsp1109I GCAGC 9 cut(s) 168, 240, 249, 346, 540, 697, 836, 1090, 1184
LweI GCATC 6 cut(s) 466, 538, 697, 882, 935, 1195
MaeI CTAG 3 cut(s) 614, 821, 1265
MaeII ACGT 4 cut(s) 135, 249, 418, 1217
MaeIII GTNAC 6 cut(s) 553, 595, 800, 816, 973, 1157
MalI GATC 6 cut(s) 209, 297, 510, 673, 946, 1263
MboI GATC 6 cut(s) 207, 295, 508, 671, 944, 1261
MboII GAAGA 5 cut(s) 244, 302, 371, 640, 866
MflI RGATCY 4 cut(s) 295, 508, 671, 1261
MhlI GDGCHC 2 cut(s) 306, 1025
MluCI AATT 3 cut(s) 24, 154, 619
MlyI GAGTC 2 cut(s) 44, 374
MmeI TCCRAC 1 cut(s) 84
MroNI GCCGGC 1 cut(s) 224
MseI TTAA 3 cut(s) 768, 842, 1122
MslI CAYNNNNRTG 1 cut(s) 1252
MspI CCGG 4 cut(s) 15, 146, 225, 234
MspR9I CCNGG 5 cut(s) 15, 147, 324, 497, 1155
Mva1269I GAATGC 1 cut(s) 489
MvaI CCWGG 3 cut(s) 324, 497, 1155
MvnI CGCG 2 cut(s) 243, 438
MwoI GCNNNNNNNGC 9 cut(s) 234, 237, 398, 534, 710, 716, 830, 1109, 1181
NaeI GCCGGC 1 cut(s) 226
NciI CCSGG 2 cut(s) 15, 147
NcoI CCATGG 1 cut(s) 123
NdeI CATATG 1 cut(s) 904
NdeII GATC 6 cut(s) 207, 295, 508, 671, 944, 1261
NgoMIV GCCGGC 1 cut(s) 224
NlaIII CATG 5 cut(s) 127, 142, 1008, 1018, 1261
NlaIV GGNNCC 2 cut(s) 348, 1070
NmeAIII GCCGAG 1 cut(s) 388
NmuCI GTSAC 1 cut(s) 595
NspI RCATGY 1 cut(s) 1008
PaeR7I CTCGAG 2 cut(s) 299, 828
PciI ACATGT 1 cut(s) 1004
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PctI GAATGC 1 cut(s) 489
PdiI GCCGGC 1 cut(s) 226
PfeI GAWTC 3 cut(s) 37, 763, 857
PleI GAGTC 2 cut(s) 44, 373
PpsI GAGTC 2 cut(s) 44, 373
PpuMI RGGWCCY 1 cut(s) 346
PscI ACATGT 1 cut(s) 1004
PsiI TTATAA 1 cut(s) 921
Psp124BI GAGCTC 1 cut(s) 306
Psp5II RGGWCCY 1 cut(s) 346
Psp6I CCWGG 3 cut(s) 322, 495, 1153
PspGI CCWGG 3 cut(s) 322, 495, 1153
PspN4I GGNNCC 2 cut(s) 348, 1070
PspPI GGNCC 2 cut(s) 127, 346
PspPPI RGGWCCY 1 cut(s) 346
PspXI VCTCGAGB 1 cut(s) 828
PstNI CAGNNNCTG 1 cut(s) 644
PsuI RGATCY 4 cut(s) 295, 508, 671, 1261
RsaI GTAC 1 cut(s) 47
RsaNI GTAC 1 cut(s) 46
RseI CAYNNNNRTG 1 cut(s) 1252
SacI GAGCTC 1 cut(s) 306
SaqAI TTAA 3 cut(s) 768, 842, 1122
Sau3AI GATC 6 cut(s) 207, 295, 508, 671, 944, 1261
Sau96I GGNCC 2 cut(s) 127, 346
SchI GAGTC 2 cut(s) 44, 374
ScrFI CCNGG 5 cut(s) 15, 147, 324, 497, 1155
SduI GDGCHC 2 cut(s) 306, 1025
SfaNI GCATC 6 cut(s) 466, 538, 697, 882, 935, 1195
SfcI CTRYAG 1 cut(s) 645
Sfr274I CTCGAG 2 cut(s) 299, 828
SinI GGWCC 2 cut(s) 127, 346
SlaI CTCGAG 2 cut(s) 299, 828
SmiMI CAYNNNNRTG 1 cut(s) 1252
SmlI CTYRAG 5 cut(s) 5, 299, 573, 828, 1199
SmoI CTYRAG 5 cut(s) 5, 299, 573, 828, 1199
Sse9I AATT 3 cut(s) 24, 154, 619
SsiI CCGC 6 cut(s) 175, 241, 436, 487, 541, 704
SspI AATATT 1 cut(s) 635
SspMI CTAG 3 cut(s) 614, 821, 1265
SstI GAGCTC 1 cut(s) 306
StyD4I CCNGG 5 cut(s) 13, 145, 322, 495, 1153
StyI CCWWGG 4 cut(s) 123, 342, 408, 661
TaaI ACNGT 5 cut(s) 559, 646, 800, 893, 1046
TaiI ACGT 4 cut(s) 138, 252, 421, 1220
TaqI TCGA 4 cut(s) 261, 300, 384, 829
TasI AATT 3 cut(s) 24, 154, 619
TauI GCSGC 1 cut(s) 243
TfiI GAWTC 3 cut(s) 37, 763, 857
Tru1I TTAA 3 cut(s) 768, 842, 1122
Tru9I TTAA 3 cut(s) 768, 842, 1122
TscAI CASTG 1 cut(s) 596
TseFI GTSAC 1 cut(s) 595
TseI GCWGC 9 cut(s) 181, 228, 237, 359, 528, 710, 824, 1103, 1172
Tsp45I GTSAC 1 cut(s) 595
TspDTI ATGAA 3 cut(s) 776, 1031, 1074
TspRI CASTG 1 cut(s) 596
VneI GTGCAC 1 cut(s) 1021
VpaK11BI GGWCC 2 cut(s) 127, 346
XapI RAATTY 1 cut(s) 619
XceI RCATGY 1 cut(s) 1008
XhoI CTCGAG 2 cut(s) 299, 828
XmiI GTMKAC 1 cut(s) 1269
XspI CTAG 3 cut(s) 614, 821, 1265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.