pycom14g19550
MYB Family

Myb-related protein 308-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0001619
Physical Location & Seq
Forward (+)
20824651 .. 20826484
1834 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 744 bp
ATGACGTTGTCAGAAAACTTTGTTATATTGTTCTCTAGCTCCCTTCACTTGGGCAGCTTGAGTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGTGCATTTGCTGTAAGAAGAACTTCAATATTACTACTGTAATGAGGAAACCTTGCTGTGAAAAGAAAAAGACGAACAAAGGAGCATGGTCGAAGCAAGAAGATAAGAAGCTCACTGAATTTGTTGAAAAAAATGGCGAAGGAAGCTGGCGCTCCCTTCCACTTGCTGCAGGCTTGCTCCGTTGTGGAAAGAGTTGTAGATTAAGATGGGTAAATTATCTAAGGCCAAACTTGAAACGTGGCAACTTTGGTGAAGATGAAGAAGACCTAATCATCAAGCTCCATGCACTTCTTGGAAACAGGTGGTCGCTAATCGCTGGAAGATTGCCGGGACGAACTGACAATGAAGTGAAGAATTACTGGAACACTCATCTAAGGAGAAAACTAATACAAATGGGAGTTGACCCCAATAACCATCGCATAGGACACACACAGAATATTGGCCTTTCAAAATCATCATTTGGCTCCAGAAAAGTAAATCATCCATGTAAAGCAGCGAATTCTCAAGGGTACAACGACTCTGATGATCATCAGAAACCATTTACAGATTCTGCAAGTGGCCCGGAAAGCAACACAAGCTGCAGTGGTTTGCCTGACCTAAATCTTGATCTCACAATAGGCCTTCCAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

248

Amino Acids

27.48

Weight (kDa)

9.39

Isoelectric Point (pI)

37.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 64 - 111 8.3e-14 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 67 - 125 1.5e-09 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 117 - 162 1.9e-17 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 122 - 165 3e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 2 cut(s) 230, 610
AfaI GTAC 1 cut(s) 623
AfiI CCNNNNNNNGG 1 cut(s) 49
AgsI TTSAA 4 cut(s) 139, 239, 346, 561
AluBI AGCT 6 cut(s) 39, 57, 223, 258, 391, 690
AluI AGCT 6 cut(s) 39, 57, 223, 258, 391, 690
AlwNI CAGNNNCTG 1 cut(s) 662
AoxI GGCC 4 cut(s) 335, 553, 670, 730
ApeKI GCWGC 4 cut(s) 54, 278, 605, 690
ApoI RAATTY 2 cut(s) 230, 610
Asp700I GAANNNNTTC 1 cut(s) 134
AspLEI GCGC 1 cut(s) 264
AspS9I GGNCC 1 cut(s) 671
AsuC2I CCSGG 2 cut(s) 441, 674
AsuHPI GGTGA 1 cut(s) 374
BbsI GAAGAC 1 cut(s) 381
BbvI GCAGC 4 cut(s) 66, 265, 617, 677
BccI CCATC 2 cut(s) 312, 534
BclI TGATCA 1 cut(s) 637
BcnI CCSGG 2 cut(s) 441, 674
BfaI CTAG 1 cut(s) 36
BfmI CTRYAG 2 cut(s) 279, 691
BfoI RGCGCY 1 cut(s) 265
BisI GCNGC 4 cut(s) 55, 279, 606, 691
BlsI GCNGC 4 cut(s) 56, 280, 607, 692
Bme1390I CCNGG 2 cut(s) 441, 674
BmgT120I GGNCC 1 cut(s) 671
BmiI GGNNCC 1 cut(s) 577
BmrFI CCNGG 2 cut(s) 441, 674
BpiI GAAGAC 1 cut(s) 381
BplI GAGNNNNNCTC 2 cut(s) 52, 84
BpmI CTGGAG 1 cut(s) 562
BpuEI CTTGAG 2 cut(s) 79, 600
BpuMI CCSGG 2 cut(s) 441, 674
BsaBI GATNNNNATC 1 cut(s) 639
Bsc4I CCNNNNNNNGG 1 cut(s) 49
Bse1I ACTGG 1 cut(s) 476
Bse8I GATNNNNATC 1 cut(s) 639
BseGI GGATG 1 cut(s) 592
BseJI GATNNNNATC 1 cut(s) 639
BseLI CCNNNNNNNGG 1 cut(s) 49
BseNI ACTGG 1 cut(s) 476
BseXI GCAGC 4 cut(s) 66, 265, 617, 677
BshFI GGCC 4 cut(s) 337, 555, 672, 732
BsiSI CCGG 2 cut(s) 440, 674
BslFI GGGAC 1 cut(s) 456
BslI CCNNNNNNNGG 1 cut(s) 49
BsmFI GGGAC 1 cut(s) 456
BsnI GGCC 4 cut(s) 337, 555, 672, 732
Bsp143I GATC 2 cut(s) 637, 718
BspANI GGCC 4 cut(s) 337, 555, 672, 732
BspLI GGNNCC 1 cut(s) 577
BspMAI CTGCAG 2 cut(s) 283, 695
BsrI ACTGG 1 cut(s) 476
BssMI GATC 2 cut(s) 637, 718
Bst4CI ACNGT 1 cut(s) 151
BstC8I GCNNGC 3 cut(s) 260, 283, 287
BstDEI CTNAG 2 cut(s) 332, 485
BstF5I GGATG 1 cut(s) 592
BstH2I RGCGCY 1 cut(s) 265
BstHHI GCGC 1 cut(s) 264
BstKTI GATC 2 cut(s) 640, 721
BstMBI GATC 2 cut(s) 637, 718
BstMWI GCNNNNNNNGC 3 cut(s) 255, 678, 687
BstSCI CCNGG 2 cut(s) 439, 672
BstSFI CTRYAG 2 cut(s) 279, 691
BstV1I GCAGC 4 cut(s) 66, 265, 617, 677
BstV2I GAAGAC 1 cut(s) 381
BsuRI GGCC 4 cut(s) 337, 555, 672, 732
BtgZI GCGATG 1 cut(s) 512
BtsCI GGATG 1 cut(s) 592
BtsI GCAGTG 1 cut(s) 700
BtsIMutI CAGTG 2 cut(s) 225, 700
Cac8I GCNNGC 3 cut(s) 260, 283, 287
CaiI CAGNNNCTG 1 cut(s) 662
CfoI GCGC 1 cut(s) 264
Cfr13I GGNCC 1 cut(s) 671
Csp6I GTAC 1 cut(s) 622
CviAII CATG 3 cut(s) 198, 395, 597
CviQI GTAC 1 cut(s) 622
DdeI CTNAG 2 cut(s) 332, 485
DpnI GATC 2 cut(s) 639, 720
DpnII GATC 2 cut(s) 637, 718
Eco147I AGGCCT 1 cut(s) 732
EcoRI GAATTC 1 cut(s) 610
FaeI CATG 3 cut(s) 201, 398, 600
FaiI YATR 5 cut(s) 26, 199, 396, 533, 598
FalI AAGNNNNNCTT 2 cut(s) 119, 151
FaqI GGGAC 1 cut(s) 456
FatI CATG 3 cut(s) 197, 394, 596
FbaI TGATCA 1 cut(s) 637
Fnu4HI GCNGC 4 cut(s) 55, 279, 606, 691
FokI GGATG 1 cut(s) 579
Fsp4HI GCNGC 4 cut(s) 55, 279, 606, 691
FspBI CTAG 1 cut(s) 36
GlaI GCGC 1 cut(s) 263
GluI GCNGC 4 cut(s) 55, 279, 606, 691
GsuI CTGGAG 1 cut(s) 562
HaeII RGCGCY 1 cut(s) 265
HaeIII GGCC 4 cut(s) 337, 555, 672, 732
HapII CCGG 2 cut(s) 440, 674
HhaI GCGC 1 cut(s) 264
Hin1II CATG 3 cut(s) 201, 398, 600
Hin6I GCGC 1 cut(s) 262
HinP1I GCGC 1 cut(s) 262
HincII GTYRAC 1 cut(s) 514
HindII GTYRAC 1 cut(s) 514
HinfI GANTC 2 cut(s) 629, 659
HpaII CCGG 2 cut(s) 440, 674
HphI GGTGA 1 cut(s) 374
Hpy166II GTNNAC 1 cut(s) 514
Hpy188I TCNGA 3 cut(s) 13, 634, 645
Hpy188III TCNNGA 2 cut(s) 579, 716
Hpy8I GTNNAC 1 cut(s) 514
HpyAV CCTTC 4 cut(s) 53, 245, 278, 743
HpyCH4III ACNGT 1 cut(s) 151
HpyCH4IV ACGT 2 cut(s) 5, 349
HpyCH4V TGCA 5 cut(s) 117, 281, 398, 665, 693
HpyF10VI GCNNNNNNNGC 3 cut(s) 255, 678, 687
HpyF3I CTNAG 2 cut(s) 332, 485
HpySE526I ACGT 2 cut(s) 5, 349
Hsp92II CATG 3 cut(s) 201, 398, 600
HspAI GCGC 1 cut(s) 262
Ksp22I TGATCA 1 cut(s) 637
Kzo9I GATC 2 cut(s) 637, 718
LmnI GCTCC 6 cut(s) 44, 194, 269, 294, 396, 581
LpnPI CCDG 9 cut(s) 244, 267, 397, 414, 453, 457, 592, 687, 717
Lsp1109I GCAGC 4 cut(s) 66, 265, 617, 677
MaeI CTAG 1 cut(s) 36
MaeII ACGT 2 cut(s) 5, 349
MalI GATC 2 cut(s) 639, 720
MboI GATC 2 cut(s) 637, 718
MboII GAAGA 7 cut(s) 142, 224, 377, 383, 386, 444, 475
MluCI AATT 4 cut(s) 230, 325, 466, 610
MlyI GAGTC 1 cut(s) 623
MnlI CCTC 1 cut(s) 150
MroXI GAANNNNTTC 1 cut(s) 134
MseI TTAA 2 cut(s) 314, 742
MspI CCGG 2 cut(s) 440, 674
MspR9I CCNGG 2 cut(s) 441, 674
MwoI GCNNNNNNNGC 3 cut(s) 255, 678, 687
NciI CCSGG 2 cut(s) 441, 674
NdeII GATC 2 cut(s) 637, 718
NlaIII CATG 3 cut(s) 201, 398, 600
NlaIV GGNNCC 1 cut(s) 577
PceI AGGCCT 1 cut(s) 732
PdmI GAANNNNTTC 1 cut(s) 134
PfeI GAWTC 1 cut(s) 659
PflFI GACNNNGTC 1 cut(s) 7
PkrI GCNGC 4 cut(s) 56, 280, 607, 692
PleI GAGTC 1 cut(s) 623
PpsI GAGTC 1 cut(s) 623
PspN4I GGNNCC 1 cut(s) 577
PspPI GGNCC 1 cut(s) 671
PstI CTGCAG 2 cut(s) 283, 695
PstNI CAGNNNCTG 1 cut(s) 662
PsyI GACNNNGTC 1 cut(s) 7
RsaI GTAC 1 cut(s) 623
RsaNI GTAC 1 cut(s) 622
SaqAI TTAA 2 cut(s) 314, 742
SatI GCNGC 4 cut(s) 55, 279, 606, 691
Sau3AI GATC 2 cut(s) 637, 718
Sau96I GGNCC 1 cut(s) 671
SchI GAGTC 1 cut(s) 623
ScrFI CCNGG 2 cut(s) 441, 674
SfcI CTRYAG 2 cut(s) 279, 691
SmlI CTYRAG 2 cut(s) 58, 615
SmoI CTYRAG 2 cut(s) 58, 615
Sse9I AATT 4 cut(s) 230, 325, 466, 610
SseBI AGGCCT 1 cut(s) 732
SspI AATATT 2 cut(s) 142, 550
SspMI CTAG 1 cut(s) 36
StuI AGGCCT 1 cut(s) 732
StyD4I CCNGG 2 cut(s) 439, 672
TaaI ACNGT 1 cut(s) 151
TaiI ACGT 2 cut(s) 8, 352
TaqI TCGA 1 cut(s) 203
TasI AATT 4 cut(s) 230, 325, 466, 610
TfiI GAWTC 1 cut(s) 659
Tru1I TTAA 2 cut(s) 314, 742
Tru9I TTAA 2 cut(s) 314, 742
TscAI CASTG 2 cut(s) 232, 700
TseI GCWGC 4 cut(s) 54, 278, 605, 690
TspDTI ATGAA 2 cut(s) 384, 471
TspGWI ACGGA 1 cut(s) 281
TspRI CASTG 2 cut(s) 232, 700
Tth111I GACNNNGTC 1 cut(s) 7
XapI RAATTY 2 cut(s) 230, 610
XcmI CCANNNNNNNNNTGG 1 cut(s) 401
XmnI GAANNNNTTC 1 cut(s) 134
XspI CTAG 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.