pycom15g02140

isoflavone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0003310
Physical Location & Seq
Reverse (-)
1263503 .. 1265229
1727 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 927 bp
ATGGCAGAAAAGAGTAAAATTTTGATCATCGGAGGCACTGGCTATATCGGAAAGTTTATCGTCGAAGCGAGCGCAAAGGCCGGCCATCCAACCTTTGCTCTAGTTAGAGAGAGCACGGTCAAAGACCCTGCCAAGGCTAACCTCATTGAGAAGTTTAAGAACTTGGGTGTCACTTTGCTCTACGGAGATCTTTATGACCATGAGAGTTTGGTGAAGGCGATCAAACAGGTGGATGTTGTGATATCGACCGTGGGTTTCATGCAAACTGCAGACCAAACCAAGATCATTGCTGCCATTAAAGAAGCTGGCAATATCAAGAGATTCTTCCCGTCGGAGTTTGGAAACGATGTGGATCGAGTGCATGCAGTTGAACCAGCCAAGTCTACGTTCGACCTCAAGGTCCAAATCCGGCGTGCTATTGAGGCCGAGGGCATCCCCTACACTTATGTCTCCAGCAACTGCTTTGCTGGCTACTTTCTGCCCTCATTGGCACAGCCAGGTGCCACTTCTCCACCCAGAGACAAAGTCACCATCTTAGGGGACGGAAATCCCAAAGCTGTGTTCAACAAGGAAGATGACATTGGAACCTATACGATCTGCGCCGTTGATGACCCAAGAACATTGAACAAGATCCTCTACCTGAAGCCTCCACAGAACATCTACTCCTTCAATGAGCTTGTTGCACTGTGGGAGAAGAAGATTGGCAAAGTTCTTGAAAAAGTCTACGTCCCAGAGGACAAACTTCTCAAGGATATTGCAGAGGCCCCAATCCCAATCAATGTGATATTAGCAATCAACCATTCAGTGTTTGTGAAGGGAGATCATACCAACTTCGAAATCGAGCCGTCCTTCGGAGTTGAGGCTTATGAGCTCTACCCTGATGTGAAGTACACCACCGTGGATGAGTACCTTGATCAATTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006979 GO:0008150 GO:0010035 GO:0010038 GO:0016020 GO:0042221 GO:0044464 GO:0046686 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NmrA

Protein Analysis

309

Amino Acids

34.12

Weight (kDa)

5.52

Isoelectric Point (pI)

26.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 3 - 304 7.6e-102 NmrA-like family
Epimerase PF01370 7 - 85 1.4e-08 NAD dependent epimerase/dehydratase family
Sacchrp_dh_NADP PF03435 7 - 96 4e-06 Saccharopine dehydrogenase NADP binding domain
3Beta_HSD PF01073 8 - 91 9.4e-07 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 8 - 78 1.3e-06 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 11 - 151 8.3e-14 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 398
AccB1I GGYRCC 1 cut(s) 500
AccI GTMKAC 2 cut(s) 383, 723
AclWI GGATC 2 cut(s) 360, 625
AcoI YGGCCR 1 cut(s) 82
AcsI RAATTY 1 cut(s) 18
AcuI CTGAAG 1 cut(s) 662
AfaI GTAC 2 cut(s) 890, 908
AfiI CCNNNNNNNGG 3 cut(s) 133, 537, 851
AgsI TTSAA 5 cut(s) 371, 565, 625, 670, 716
AjnI CCWGG 1 cut(s) 496
AjuI GAANNNNNNNTTGG 6 cut(s) 371, 403, 545, 564, 577, 596
AleI CACNNNNGTG 1 cut(s) 896
AloI GAACNNNNNNTCC 2 cut(s) 647, 679
AluBI AGCT 4 cut(s) 305, 557, 676, 871
AluI AGCT 4 cut(s) 305, 557, 676, 871
Alw21I GWGCWC 2 cut(s) 116, 873
Alw26I GTCTC 2 cut(s) 454, 513
AlwI GGATC 2 cut(s) 360, 625
AlwNI CAGNNNCTG 1 cut(s) 459
AoxI GGCC 4 cut(s) 78, 82, 423, 762
ApeKI GCWGC 1 cut(s) 290
ApoI RAATTY 1 cut(s) 18
AspLEI GCGC 2 cut(s) 74, 602
AspS9I GGNCC 2 cut(s) 400, 763
AsuHPI GGTGA 2 cut(s) 223, 520
AsuII TTCGAA 1 cut(s) 834
AvaII GGWCC 1 cut(s) 400
BanI GGYRCC 1 cut(s) 500
BanII GRGCYC 1 cut(s) 873
Bbv12I GWGCWC 2 cut(s) 116, 873
BbvI GCAGC 1 cut(s) 277
BccI CCATC 2 cut(s) 93, 539
BceAI ACGGC 2 cut(s) 587, 829
BciT130I CCWGG 1 cut(s) 498
BclI TGATCA 2 cut(s) 24, 913
BcoDI GTCTC 2 cut(s) 454, 513
BfaI CTAG 1 cut(s) 101
BfmI CTRYAG 1 cut(s) 267
BglII AGATCT 1 cut(s) 187
BisI GCNGC 1 cut(s) 291
BlsI GCNGC 1 cut(s) 292
Bme1390I CCNGG 1 cut(s) 498
Bme18I GGWCC 1 cut(s) 400
BmgT120I GGNCC 2 cut(s) 400, 763
BmiI GGNNCC 3 cut(s) 502, 586, 765
BmrFI CCNGG 1 cut(s) 498
BmsI GCATC 1 cut(s) 441
BpmI CTGGAG 1 cut(s) 436
Bpu14I TTCGAA 1 cut(s) 834
BpuEI CTTGAG 2 cut(s) 380, 731
BsaBI GATNNNNATC 1 cut(s) 351
BsaJI CCNNGG 4 cut(s) 132, 249, 426, 897
BsaXI ACNNNNNCTCC 2 cut(s) 647, 677
Bsc4I CCNNNNNNNGG 3 cut(s) 133, 537, 851
Bse118I RCCGGY 1 cut(s) 80
Bse1I ACTGG 1 cut(s) 43
Bse3DI GCAATG 1 cut(s) 285
Bse8I GATNNNNATC 1 cut(s) 351
BseBI CCWGG 1 cut(s) 498
BseDI CCNNGG 4 cut(s) 132, 249, 426, 897
BseGI GGATG 4 cut(s) 85, 238, 432, 907
BseJI GATNNNNATC 1 cut(s) 351
BseLI CCNNNNNNNGG 3 cut(s) 133, 537, 851
BseMI GCAATG 1 cut(s) 285
BseNI ACTGG 1 cut(s) 43
BseXI GCAGC 1 cut(s) 277
Bsh1285I CGRYCG 1 cut(s) 249
BshFI GGCC 4 cut(s) 80, 84, 425, 764
BshNI GGYRCC 1 cut(s) 500
BsiEI CGRYCG 1 cut(s) 249
BsiHKAI GWGCWC 2 cut(s) 116, 873
BsiSI CCGG 2 cut(s) 81, 409
BslFI GGGAC 2 cut(s) 554, 713
BslI CCNNNNNNNGG 3 cut(s) 133, 537, 851
BsmAI GTCTC 2 cut(s) 454, 513
BsmFI GGGAC 2 cut(s) 554, 713
BsnI GGCC 4 cut(s) 80, 84, 425, 764
Bsp119I TTCGAA 1 cut(s) 834
Bsp1286I GDGCHC 2 cut(s) 116, 873
Bsp143I GATC 9 cut(s) 24, 187, 219, 282, 352, 594, 630, 820, 913
BspANI GGCC 4 cut(s) 80, 84, 425, 764
BspLI GGNNCC 3 cut(s) 502, 586, 765
BspMAI CTGCAG 1 cut(s) 271
BspPI GGATC 2 cut(s) 360, 625
BspT104I TTCGAA 1 cut(s) 834
BspT107I GGYRCC 1 cut(s) 500
BsrDI GCAATG 1 cut(s) 285
BsrFI RCCGGY 1 cut(s) 80
BsrI ACTGG 1 cut(s) 43
BssAI RCCGGY 1 cut(s) 80
BssECI CCNNGG 4 cut(s) 132, 249, 426, 897
BssMI GATC 9 cut(s) 24, 187, 219, 282, 352, 594, 630, 820, 913
BssT1I CCWWGG 1 cut(s) 132
Bst2UI CCWGG 1 cut(s) 498
Bst4CI ACNGT 4 cut(s) 118, 250, 687, 898
BstBI TTCGAA 1 cut(s) 834
BstC8I GCNNGC 6 cut(s) 70, 82, 307, 363, 414, 469
BstDEI CTNAG 1 cut(s) 535
BstDSI CCRYGG 2 cut(s) 249, 897
BstF5I GGATG 4 cut(s) 85, 238, 432, 907
BstHHI GCGC 2 cut(s) 74, 602
BstKTI GATC 9 cut(s) 27, 190, 222, 285, 355, 597, 633, 823, 916
BstMAI GTCTC 2 cut(s) 454, 513
BstMBI GATC 9 cut(s) 24, 187, 219, 282, 352, 594, 630, 820, 913
BstMCI CGRYCG 1 cut(s) 249
BstMWI GCNNNNNNNGC 2 cut(s) 422, 468
BstNI CCWGG 1 cut(s) 498
BstNSI RCATGY 1 cut(s) 365
BstSCI CCNGG 1 cut(s) 496
BstSFI CTRYAG 1 cut(s) 267
BstV1I GCAGC 1 cut(s) 277
BstX2I RGATCY 2 cut(s) 187, 630
BstYI RGATCY 2 cut(s) 187, 630
BsuRI GGCC 4 cut(s) 80, 84, 425, 764
BtgI CCRYGG 2 cut(s) 249, 897
BtsCI GGATG 4 cut(s) 85, 238, 432, 907
BtsIMutI CAGTG 3 cut(s) 36, 683, 810
Cac8I GCNNGC 6 cut(s) 70, 82, 307, 363, 414, 469
CaiI CAGNNNCTG 1 cut(s) 459
CfoI GCGC 2 cut(s) 74, 602
Cfr10I RCCGGY 1 cut(s) 80
Cfr13I GGNCC 2 cut(s) 400, 763
Csp6I GTAC 2 cut(s) 889, 907
CviAII CATG 3 cut(s) 200, 259, 362
CviQI GTAC 2 cut(s) 889, 907
DdeI CTNAG 1 cut(s) 535
DpnI GATC 9 cut(s) 26, 189, 221, 284, 354, 596, 632, 822, 915
DpnII GATC 9 cut(s) 24, 187, 219, 282, 352, 594, 630, 820, 913
DrdI GACNNNNNNGTC 1 cut(s) 398
DseDI GACNNNNNNGTC 1 cut(s) 398
EaeI YGGCCR 1 cut(s) 82
Ecl136II GAGCTC 1 cut(s) 871
Eco130I CCWWGG 1 cut(s) 132
Eco24I GRGCYC 1 cut(s) 873
Eco32I GATATC 1 cut(s) 243
Eco47I GGWCC 1 cut(s) 400
Eco53kI GAGCTC 1 cut(s) 871
Eco57I CTGAAG 1 cut(s) 662
EcoICRI GAGCTC 1 cut(s) 871
EcoO109I RGGNCCY 1 cut(s) 763
EcoRII CCWGG 1 cut(s) 496
EcoRV GATATC 1 cut(s) 243
EcoT14I CCWWGG 1 cut(s) 132
EcoT38I GRGCYC 1 cut(s) 873
ErhI CCWWGG 1 cut(s) 132
FaeI CATG 3 cut(s) 203, 262, 365
FaiI YATR 9 cut(s) 45, 195, 201, 260, 363, 447, 591, 825, 867
FalI AAGNNNNNCTT 2 cut(s) 308, 340
FaqI GGGAC 2 cut(s) 554, 713
FatI CATG 3 cut(s) 199, 258, 361
FbaI TGATCA 2 cut(s) 24, 913
FblI GTMKAC 2 cut(s) 383, 723
Fnu4HI GCNGC 1 cut(s) 291
FokI GGATG 4 cut(s) 72, 245, 419, 914
FriOI GRGCYC 1 cut(s) 873
FseI GGCCGGCC 1 cut(s) 84
Fsp4HI GCNGC 1 cut(s) 291
FspBI CTAG 1 cut(s) 101
GlaI GCGC 2 cut(s) 73, 601
GluI GCNGC 1 cut(s) 291
GsuI CTGGAG 1 cut(s) 436
HaeIII GGCC 4 cut(s) 80, 84, 425, 764
HapII CCGG 2 cut(s) 81, 409
HhaI GCGC 2 cut(s) 74, 602
Hin1II CATG 3 cut(s) 203, 262, 365
Hin6I GCGC 2 cut(s) 72, 600
HinP1I GCGC 2 cut(s) 72, 600
HinfI GANTC 1 cut(s) 321
HpaII CCGG 2 cut(s) 81, 409
HphI GGTGA 2 cut(s) 223, 520
Hpy166II GTNNAC 3 cut(s) 384, 724, 891
Hpy188I TCNGA 4 cut(s) 32, 50, 334, 854
Hpy188III TCNNGA 2 cut(s) 316, 713
Hpy8I GTNNAC 3 cut(s) 384, 724, 891
Hpy99I CGWCG 2 cut(s) 65, 334
HpyAV CCTTC 4 cut(s) 208, 676, 808, 859
HpyCH4III ACNGT 4 cut(s) 118, 250, 687, 898
HpyCH4IV ACGT 2 cut(s) 386, 726
HpyCH4V TGCA 6 cut(s) 262, 269, 361, 365, 683, 758
HpyF10VI GCNNNNNNNGC 2 cut(s) 422, 468
HpyF3I CTNAG 1 cut(s) 535
HpySE526I ACGT 2 cut(s) 386, 726
Hsp92II CATG 3 cut(s) 203, 262, 365
HspAI GCGC 2 cut(s) 72, 600
KroI GCCGGC 1 cut(s) 80
KroNI GCCGGC 1 cut(s) 82
Ksp22I TGATCA 2 cut(s) 24, 913
Kzo9I GATC 9 cut(s) 24, 187, 219, 282, 352, 594, 630, 820, 913
Lsp1109I GCAGC 1 cut(s) 277
LweI GCATC 1 cut(s) 441
MaeI CTAG 1 cut(s) 101
MaeII ACGT 2 cut(s) 386, 726
MaeIII GTNAC 2 cut(s) 169, 526
MalI GATC 9 cut(s) 26, 189, 221, 284, 354, 596, 632, 822, 915
MboI GATC 9 cut(s) 24, 187, 219, 282, 352, 594, 630, 820, 913
MboII GAAGA 4 cut(s) 316, 584, 706, 709
MflI RGATCY 2 cut(s) 187, 630
MhlI GDGCHC 2 cut(s) 116, 873
MluCI AATT 2 cut(s) 18, 917
MmeI TCCRAC 2 cut(s) 113, 312
MroNI GCCGGC 1 cut(s) 80
MseI TTAA 2 cut(s) 156, 297
MslI CAYNNNNRTG 1 cut(s) 896
MspI CCGG 2 cut(s) 81, 409
MspR9I CCNGG 1 cut(s) 498
MvaI CCWGG 1 cut(s) 498
MwoI GCNNNNNNNGC 2 cut(s) 422, 468
NaeI GCCGGC 1 cut(s) 82
NdeII GATC 9 cut(s) 24, 187, 219, 282, 352, 594, 630, 820, 913
NgoMIV GCCGGC 1 cut(s) 80
NlaIII CATG 3 cut(s) 203, 262, 365
NlaIV GGNNCC 3 cut(s) 502, 586, 765
NmeAIII GCCGAG 1 cut(s) 451
NmuCI GTSAC 2 cut(s) 169, 526
NspI RCATGY 1 cut(s) 365
NspV TTCGAA 1 cut(s) 834
OliI CACNNNNGTG 1 cut(s) 896
PaeI GCATGC 1 cut(s) 365
PdiI GCCGGC 1 cut(s) 82
PfeI GAWTC 1 cut(s) 321
PflFI GACNNNGTC 1 cut(s) 524
PkrI GCNGC 1 cut(s) 292
Psp124BI GAGCTC 1 cut(s) 873
Psp6I CCWGG 1 cut(s) 496
PspGI CCWGG 1 cut(s) 496
PspN4I GGNNCC 3 cut(s) 502, 586, 765
PspPI GGNCC 2 cut(s) 400, 763
PstI CTGCAG 1 cut(s) 271
PstNI CAGNNNCTG 1 cut(s) 459
PsuI RGATCY 2 cut(s) 187, 630
PsyI GACNNNGTC 1 cut(s) 524
RigI GGCCGGCC 1 cut(s) 84
RsaI GTAC 2 cut(s) 890, 908
RsaNI GTAC 2 cut(s) 889, 907
RseI CAYNNNNRTG 1 cut(s) 896
SacI GAGCTC 1 cut(s) 873
SaqAI TTAA 2 cut(s) 156, 297
SatI GCNGC 1 cut(s) 291
Sau3AI GATC 9 cut(s) 24, 187, 219, 282, 352, 594, 630, 820, 913
Sau96I GGNCC 2 cut(s) 400, 763
ScrFI CCNGG 1 cut(s) 498
SduI GDGCHC 2 cut(s) 116, 873
SfaNI GCATC 1 cut(s) 441
SfcI CTRYAG 1 cut(s) 267
SfuI TTCGAA 1 cut(s) 834
SinI GGWCC 1 cut(s) 400
SmiMI CAYNNNNRTG 1 cut(s) 896
SmlI CTYRAG 2 cut(s) 395, 746
SmoI CTYRAG 2 cut(s) 395, 746
SphI GCATGC 1 cut(s) 365
Sse9I AATT 2 cut(s) 18, 917
SspMI CTAG 1 cut(s) 101
SstI GAGCTC 1 cut(s) 873
StyD4I CCNGG 1 cut(s) 496
StyI CCWWGG 1 cut(s) 132
TaaI ACNGT 4 cut(s) 118, 250, 687, 898
TaiI ACGT 2 cut(s) 389, 729
TaqI TCGA 6 cut(s) 63, 245, 355, 390, 834, 840
TasI AATT 2 cut(s) 18, 917
TatI WGTACW 1 cut(s) 888
TfiI GAWTC 1 cut(s) 321
Tru1I TTAA 2 cut(s) 156, 297
Tru9I TTAA 2 cut(s) 156, 297
TscAI CASTG 3 cut(s) 43, 690, 810
TseFI GTSAC 2 cut(s) 169, 526
TseI GCWGC 1 cut(s) 290
Tsp45I GTSAC 2 cut(s) 169, 526
TspDTI ATGAA 1 cut(s) 247
TspGWI ACGGA 2 cut(s) 198, 558
TspRI CASTG 3 cut(s) 43, 690, 810
Tth111I GACNNNGTC 1 cut(s) 524
VpaK11BI GGWCC 1 cut(s) 400
XapI RAATTY 1 cut(s) 18
XceI RCATGY 1 cut(s) 365
XmiI GTMKAC 2 cut(s) 383, 723
XspI CTAG 1 cut(s) 101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.