pycom15g16290

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0001510
Physical Location & Seq
Reverse (-)
11419764 .. 11420844
1081 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 876 bp
ATGTGTGCAGTCAACCTGTTTAGAGTTTTTCCACCTAAGTACCGATCTAATTTTACTGGGGGTGAAACAGACGATGAAGAACCAATGTTTGATCCTTCTTGGTCATACCTGCAAATTGTGTATGATCTTCTGGTTCGGTTTATCAGTTATACTTCACTTGACATAAAGGTGGCAAAAAAGCATGTGGATCACTCTTTTATCGTAAAATTACTTGACCTCTTTGACTCTGAGGACCCAAGAGAAAGAGACTGTTTGAAAACCATTCTGCATAGGATTTATGGAAAGTTCATGGTACACAGACCCTTTATTCGGAAAGCTGTCAGCAATATCATATATCGTTTTGTTTTCGAAACTGAAAGGCATAATGGAATTTCTGAGCTGTTGGAAATTTTTGGAAGTGTAATTAGTGGGTTTGCTTTGCCATTGAAGGAGGAACACAAGATATTTTTGTGGAGGGCTCTTATTCCTCTACACAAACCAAAGTCAGTGGGTATTTATCATCAGCAATTGACATATTGTGTTGTACAGTTCATAGATAAGGATCCGAAGTTGGCAAGTACTGTAATAAAGGGTTTGTTGAAGTTCTGGCCTGTAACAAATAGCCAGAAGGAGTTGATGTTTCTAAGTGAGTTAGAAGAGGTTTTGGAAATGACTAGCATGGATGAGTTTCAAAGATCGATCATGGTTCCACTGTTCCGGCGCATAAGATGCTGCCTCAATAGTTCTCATTACCAGGTGGCTGAACGAGCCCACTTGCTGTGGAACAATGAACACATCCTTAACCTTATCGCACATAACCGCCAGGTTCGATCATTCCTCTTGTCTTCCCAGCTATTGAGCGAAACACCCAAAACCACTGGAATCAAGCAGTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 ko04071 ko04113 ko04114 ko04151 ko04152 ko04261 ko04728 ko05165 map03015 map04071 map04113 map04114 map04151 map04152 map04261 map04728 map05165
Pfam Domains
Protein Families
B56

Protein Analysis

292

Amino Acids

34.17

Weight (kDa)

8.65

Isoelectric Point (pI)

38.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B56 PF01603 1 - 277 5.3e-124 Protein phosphatase 2A regulatory B subunit (B56 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 117
AciI CCGC 1 cut(s) 799
AclWI GGATC 4 cut(s) 86, 195, 536, 549
AcsI RAATTY 2 cut(s) 369, 387
AfaI GTAC 4 cut(s) 41, 294, 525, 559
AfiI CCNNNNNNNGG 1 cut(s) 309
AgsI TTSAA 4 cut(s) 256, 427, 580, 671
AjnI CCWGG 2 cut(s) 732, 801
AluBI AGCT 3 cut(s) 317, 379, 832
AluI AGCT 3 cut(s) 317, 379, 832
Alw26I GTCTC 1 cut(s) 240
AlwI GGATC 4 cut(s) 86, 195, 536, 549
AoxI GGCC 1 cut(s) 587
ApeKI GCWGC 1 cut(s) 711
ApoI RAATTY 2 cut(s) 369, 387
ArsI GACNNNNNNTTYG 2 cut(s) 291, 323
AspLEI GCGC 1 cut(s) 702
AspS9I GGNCC 1 cut(s) 232
AsuHPI GGTGA 1 cut(s) 74
AsuII TTCGAA 1 cut(s) 348
AvaII GGWCC 1 cut(s) 232
BaeI ACNNNNGTAYC 2 cut(s) 23, 56
BamHI GGATCC 1 cut(s) 541
BanII GRGCYC 2 cut(s) 460, 751
BbsI GAAGAC 1 cut(s) 816
BbvI GCAGC 1 cut(s) 698
BciT130I CCWGG 2 cut(s) 734, 803
BcoDI GTCTC 1 cut(s) 240
BfaI CTAG 1 cut(s) 654
BfuAI ACCTGC 1 cut(s) 117
BisI GCNGC 1 cut(s) 712
BlsI GCNGC 1 cut(s) 713
BmcAI AGTACT 1 cut(s) 559
Bme1390I CCNGG 2 cut(s) 734, 803
Bme18I GGWCC 1 cut(s) 232
BmgT120I GGNCC 1 cut(s) 232
BmiI GGNNCC 3 cut(s) 234, 543, 687
BmrFI CCNGG 2 cut(s) 734, 803
BmrI ACTGGG 1 cut(s) 66
BmsI GCATC 1 cut(s) 698
BmuI ACTGGG 1 cut(s) 66
BpiI GAAGAC 1 cut(s) 816
Bpu14I TTCGAA 1 cut(s) 348
Bsa29I ATCGAT 1 cut(s) 677
BsaBI GATNNNNATC 1 cut(s) 540
BsaXI ACNNNNNCTCC 2 cut(s) 602, 632
Bsc4I CCNNNNNNNGG 1 cut(s) 309
Bse1I ACTGG 2 cut(s) 61, 862
Bse8I GATNNNNATC 1 cut(s) 540
BseBI CCWGG 2 cut(s) 734, 803
BseCI ATCGAT 1 cut(s) 677
BseGI GGATG 2 cut(s) 667, 774
BseJI GATNNNNATC 1 cut(s) 540
BseLI CCNNNNNNNGG 1 cut(s) 309
BseMII CTCAG 2 cut(s) 219, 366
BseNI ACTGG 2 cut(s) 61, 862
BseXI GCAGC 1 cut(s) 698
BseYI CCCAGC 1 cut(s) 828
BsgI GTGCAG 1 cut(s) 27
BshFI GGCC 1 cut(s) 589
BshVI ATCGAT 1 cut(s) 677
BsiSI CCGG 1 cut(s) 697
BslI CCNNNNNNNGG 1 cut(s) 309
BsmAI GTCTC 1 cut(s) 240
BsnI GGCC 1 cut(s) 589
Bsp119I TTCGAA 1 cut(s) 348
Bsp1286I GDGCHC 2 cut(s) 460, 751
Bsp1407I TGTACA 1 cut(s) 523
Bsp143I GATC 8 cut(s) 44, 91, 124, 187, 541, 674, 678, 809
BspACI CCGC 1 cut(s) 799
BspANI GGCC 1 cut(s) 589
BspCNI CTCAG 2 cut(s) 220, 367
BspDI ATCGAT 1 cut(s) 677
BspLI GGNNCC 3 cut(s) 234, 543, 687
BspMI ACCTGC 1 cut(s) 117
BspPI GGATC 4 cut(s) 86, 195, 536, 549
BspT104I TTCGAA 1 cut(s) 348
BsrGI TGTACA 1 cut(s) 523
BsrI ACTGG 2 cut(s) 61, 862
BssMI GATC 8 cut(s) 44, 91, 124, 187, 541, 674, 678, 809
Bst2UI CCWGG 2 cut(s) 734, 803
Bst4CI ACNGT 4 cut(s) 251, 528, 562, 693
Bst6I CTCTTC 1 cut(s) 630
BstAPI GCANNNNNTGC 1 cut(s) 708
BstAUI TGTACA 1 cut(s) 523
BstBI TTCGAA 1 cut(s) 348
BstDEI CTNAG 4 cut(s) 36, 228, 375, 623
BstF5I GGATG 2 cut(s) 667, 774
BstHHI GCGC 1 cut(s) 702
BstKTI GATC 8 cut(s) 47, 94, 127, 190, 544, 677, 681, 812
BstMAI GTCTC 1 cut(s) 240
BstMBI GATC 8 cut(s) 44, 91, 124, 187, 541, 674, 678, 809
BstMWI GCNNNNNNNGC 2 cut(s) 708, 746
BstNI CCWGG 2 cut(s) 734, 803
BstNSI RCATGY 1 cut(s) 185
BstSCI CCNGG 2 cut(s) 732, 801
BstV1I GCAGC 1 cut(s) 698
BstV2I GAAGAC 1 cut(s) 816
BstX2I RGATCY 1 cut(s) 541
BstYI RGATCY 1 cut(s) 541
Bsu15I ATCGAT 1 cut(s) 677
BsuRI GGCC 1 cut(s) 589
BsuTUI ATCGAT 1 cut(s) 677
BtsCI GGATG 2 cut(s) 667, 774
BtsI GCAGTG 1 cut(s) 875
BtsIMutI CAGTG 4 cut(s) 492, 689, 855, 875
BveI ACCTGC 1 cut(s) 117
CfoI GCGC 1 cut(s) 702
Cfr13I GGNCC 1 cut(s) 232
ClaI ATCGAT 1 cut(s) 677
CsiI ACCWGGT 1 cut(s) 732
Csp6I GTAC 4 cut(s) 40, 293, 524, 558
CspCI CAANNNNNGTGG 2 cut(s) 468, 503
CviAII CATG 4 cut(s) 182, 289, 658, 682
CviJI RGCY 8 cut(s) 317, 379, 458, 589, 603, 740, 749, 832
CviKI_1 RGCY 8 cut(s) 317, 379, 458, 589, 603, 740, 749, 832
CviQI GTAC 4 cut(s) 40, 293, 524, 558
DdeI CTNAG 4 cut(s) 36, 228, 375, 623
DpnI GATC 8 cut(s) 46, 93, 126, 189, 543, 676, 680, 811
DpnII GATC 8 cut(s) 44, 91, 124, 187, 541, 674, 678, 809
Eam1104I CTCTTC 1 cut(s) 630
EarI CTCTTC 1 cut(s) 630
Eco24I GRGCYC 2 cut(s) 460, 751
Eco47I GGWCC 1 cut(s) 232
EcoO109I RGGNCCY 1 cut(s) 232
EcoRII CCWGG 2 cut(s) 732, 801
EcoT38I GRGCYC 2 cut(s) 460, 751
FaeI CATG 4 cut(s) 185, 292, 661, 685
FatI CATG 4 cut(s) 181, 288, 657, 681
Fnu4HI GCNGC 1 cut(s) 712
FokI GGATG 2 cut(s) 674, 761
FriOI GRGCYC 2 cut(s) 460, 751
Fsp4HI GCNGC 1 cut(s) 712
FspBI CTAG 1 cut(s) 654
GlaI GCGC 1 cut(s) 701
GluI GCNGC 1 cut(s) 712
GsaI CCCAGC 1 cut(s) 832
HaeIII GGCC 1 cut(s) 589
HapII CCGG 1 cut(s) 697
HhaI GCGC 1 cut(s) 702
Hin1II CATG 4 cut(s) 185, 292, 661, 685
Hin6I GCGC 1 cut(s) 700
HinP1I GCGC 1 cut(s) 700
HincII GTYRAC 1 cut(s) 13
HindII GTYRAC 1 cut(s) 13
HinfI GANTC 2 cut(s) 224, 861
HpaII CCGG 1 cut(s) 697
HphI GGTGA 1 cut(s) 74
Hpy166II GTNNAC 2 cut(s) 13, 295
Hpy188I TCNGA 4 cut(s) 229, 312, 376, 546
Hpy8I GTNNAC 2 cut(s) 13, 295
HpyAV CCTTC 3 cut(s) 105, 421, 601
HpyCH4III ACNGT 4 cut(s) 251, 528, 562, 693
HpyCH4V TGCA 3 cut(s) 8, 112, 268
HpyF10VI GCNNNNNNNGC 2 cut(s) 708, 746
HpyF3I CTNAG 4 cut(s) 36, 228, 375, 623
Hsp92II CATG 4 cut(s) 185, 292, 661, 685
HspAI GCGC 1 cut(s) 700
Kzo9I GATC 8 cut(s) 44, 91, 124, 187, 541, 674, 678, 809
Lsp1109I GCAGC 1 cut(s) 698
LweI GCATC 1 cut(s) 698
MabI ACCWGGT 1 cut(s) 732
MaeI CTAG 1 cut(s) 654
MaeIII GTNAC 1 cut(s) 592
MalI GATC 8 cut(s) 46, 93, 126, 189, 543, 676, 680, 811
MboI GATC 8 cut(s) 44, 91, 124, 187, 541, 674, 678, 809
MboII GAAGA 4 cut(s) 89, 119, 647, 816
MfeI CAATTG 1 cut(s) 506
MflI RGATCY 1 cut(s) 541
MhlI GDGCHC 2 cut(s) 460, 751
MluCI AATT 7 cut(s) 49, 114, 206, 369, 387, 402, 506
MlyI GAGTC 1 cut(s) 218
MmeI TCCRAC 1 cut(s) 363
MnlI CCTC 8 cut(s) 223, 227, 424, 447, 477, 631, 725, 827
MseI TTAA 1 cut(s) 780
MslI CAYNNNNRTG 1 cut(s) 167
MspI CCGG 1 cut(s) 697
MspR9I CCNGG 2 cut(s) 734, 803
MunI CAATTG 1 cut(s) 506
MvaI CCWGG 2 cut(s) 734, 803
MwoI GCNNNNNNNGC 2 cut(s) 708, 746
NdeII GATC 8 cut(s) 44, 91, 124, 187, 541, 674, 678, 809
NlaIII CATG 4 cut(s) 185, 292, 661, 685
NlaIV GGNNCC 3 cut(s) 234, 543, 687
NspI RCATGY 1 cut(s) 185
NspV TTCGAA 1 cut(s) 348
PfeI GAWTC 1 cut(s) 861
PkrI GCNGC 1 cut(s) 713
PleI GAGTC 1 cut(s) 218
PpsI GAGTC 1 cut(s) 218
PpuMI RGGWCCY 1 cut(s) 232
Psp5II RGGWCCY 1 cut(s) 232
Psp6I CCWGG 2 cut(s) 732, 801
PspFI CCCAGC 1 cut(s) 828
PspGI CCWGG 2 cut(s) 732, 801
PspN4I GGNNCC 3 cut(s) 234, 543, 687
PspPI GGNCC 1 cut(s) 232
PspPPI RGGWCCY 1 cut(s) 232
PsuI RGATCY 1 cut(s) 541
RsaI GTAC 4 cut(s) 41, 294, 525, 559
RsaNI GTAC 4 cut(s) 40, 293, 524, 558
RseI CAYNNNNRTG 1 cut(s) 167
SaqAI TTAA 1 cut(s) 780
SatI GCNGC 1 cut(s) 712
Sau3AI GATC 8 cut(s) 44, 91, 124, 187, 541, 674, 678, 809
Sau96I GGNCC 1 cut(s) 232
ScaI AGTACT 1 cut(s) 559
SchI GAGTC 1 cut(s) 218
ScrFI CCNGG 2 cut(s) 734, 803
SduI GDGCHC 2 cut(s) 460, 751
SexAI ACCWGGT 1 cut(s) 732
SfaNI GCATC 1 cut(s) 698
SfuI TTCGAA 1 cut(s) 348
SinI GGWCC 1 cut(s) 232
SmiMI CAYNNNNRTG 1 cut(s) 167
Sse9I AATT 7 cut(s) 49, 114, 206, 369, 387, 402, 506
SsiI CCGC 1 cut(s) 799
SspMI CTAG 1 cut(s) 654
StyD4I CCNGG 2 cut(s) 732, 801
TaaI ACNGT 4 cut(s) 251, 528, 562, 693
TaqI TCGA 3 cut(s) 348, 677, 808
TasI AATT 7 cut(s) 49, 114, 206, 369, 387, 402, 506
TatI WGTACW 2 cut(s) 523, 557
TfiI GAWTC 1 cut(s) 861
Tru1I TTAA 1 cut(s) 780
Tru9I TTAA 1 cut(s) 780
TscAI CASTG 4 cut(s) 492, 696, 862, 875
TseI GCWGC 1 cut(s) 711
TspDTI ATGAA 4 cut(s) 90, 277, 520, 783
TspRI CASTG 4 cut(s) 492, 696, 862, 875
VpaK11BI GGWCC 1 cut(s) 232
XapI RAATTY 2 cut(s) 369, 387
XceI RCATGY 1 cut(s) 185
XspI CTAG 1 cut(s) 654
ZrmI AGTACT 1 cut(s) 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.