pycom15g16300

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0001510
Physical Location & Seq
Reverse (-)
11424662 .. 11427590
2929 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1017 bp
ATGAACCACCACATCAACTGTGATATAAGTTGGTATATAAATGTGATATATAAAAGATATGATATATCTAATAATATCTTGTACTCAATAAATATTGGTTTCGCTCAAGTATTCCCTGCGCGCTACTGTCAAGCCTTGCATGTCAACCGCAAGCAGTGCTCCCGCACCAGACCCCACGTGGCCTCTCCTTATTCGCCACCCCTCGTGGCCTCTCCTTTTTCGCCACCCCTCGTGCCATTGAATTTTTCTACCTCCGCCTCCCCTCCTGTTCTCAATATATTTAACAGCAAAGCAAACAACCTGCACACGTTCGCACAGTGCTGGCCGGCCGGAAAGATGATGTCAGCAAATGAAAGCATGGAGTTTAAGGACACTGAGCTTACCTTAGGGTTACCTGGTACGACGTCAGATTACAACCATCCTCGTGTCTTTGGAGCGTTCAACTTCAAGATAAGTATTACTAATAGCAGCTCGGTGAAACGTGGTTTCATGGATACCGTTGATCAGATTAGCCTGCAGAAAAGCTCTACCGCCAGCGAGCCACGTGGCAGCTCATCATCTAGTACCAGTAGTTCTACAACTACTTCCGTCATCAAAGCCGCAGCTGCAAAAGCAAGAATAGCTGGGTGGCCGCCGGTAAGATCGATGAGACAGAAGTCGTTGGTGGTGGTGGAAAGCAGCAGAAAGTATGTAAAAGTGAGCGCAGATGGAGCTCCTTATCTACGTAAACTCGACTTGGAGATGTACAAAAGTTATCAAGAGCTATTGAGAGCCTTAGAGCAAATGTTCCCTTCCTTAACCATAAGCGGTAAGTGTATCGAGGATGGCAATGAGGTTATGAAACCTGCAGTGAAAGGAATGGATCAATATATGTTGACAAATGAAGACAAGGATGGAGACTGGATGTTAGTTGGAGACGTCCCTTGGAAGATGTTTATAGAATCATGCAAGCGTATTCGGTTAATGAAAAGTTCAGATGCTGTTGGGATAGCTCCGAGGGCTCCGATACCTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AUX_IAA

Protein Analysis

339

Amino Acids

37.53

Weight (kDa)

9.39

Isoelectric Point (pI)

54.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 124 - 333 1.6e-57 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 407, 921
Acc36I ACCTGC 2 cut(s) 309, 853
AccII CGCG 1 cut(s) 121
AciI CCGC 7 cut(s) 148, 163, 255, 531, 600, 632, 807
AclWI GGATC 1 cut(s) 870
AcoI YGGCCR 3 cut(s) 323, 327, 629
AcsI RAATTY 1 cut(s) 241
AcvI CACGTG 2 cut(s) 178, 545
AcyI GRCGYC 2 cut(s) 404, 918
AfaI GTAC 4 cut(s) 83, 400, 565, 746
AflIII ACRYGT 1 cut(s) 306
AgsI TTSAA 3 cut(s) 241, 442, 448
AjnI CCWGG 1 cut(s) 394
AluBI AGCT 9 cut(s) 379, 471, 525, 552, 605, 623, 713, 763, 992
AluI AGCT 9 cut(s) 379, 471, 525, 552, 605, 623, 713, 763, 992
Alw21I GWGCWC 2 cut(s) 161, 715
Alw26I GTCTC 3 cut(s) 643, 891, 909
AlwI GGATC 1 cut(s) 870
AlwNI CAGNNNCTG 1 cut(s) 980
AoxI GGCC 5 cut(s) 180, 207, 323, 327, 629
ApeKI GCWGC 5 cut(s) 468, 549, 602, 605, 678
ApoI RAATTY 1 cut(s) 241
AspLEI GCGC 3 cut(s) 121, 123, 704
AsuHPI GGTGA 1 cut(s) 487
AxyI CCTNAGG 1 cut(s) 385
BanII GRGCYC 2 cut(s) 715, 1003
BauI CACGAG 3 cut(s) 203, 230, 423
BbrPI CACGTG 2 cut(s) 178, 545
BbsI GAAGAC 1 cut(s) 891
Bbv12I GWGCWC 2 cut(s) 161, 715
BbvI GCAGC 5 cut(s) 480, 561, 592, 614, 690
BccI CCATC 4 cut(s) 426, 701, 818, 887
BciT130I CCWGG 1 cut(s) 396
BciVI GTATCC 1 cut(s) 487
BclI TGATCA 1 cut(s) 502
BcoDI GTCTC 3 cut(s) 643, 891, 909
BfaI CTAG 1 cut(s) 561
BfmI CTRYAG 2 cut(s) 515, 846
BfuAI ACCTGC 2 cut(s) 309, 853
BfuI GTATCC 1 cut(s) 487
BisI GCNGC 7 cut(s) 469, 550, 600, 603, 606, 632, 679
BlsI GCNGC 7 cut(s) 470, 551, 601, 604, 607, 633, 680
Bme1390I CCNGG 1 cut(s) 396
BmiI GGNNCC 1 cut(s) 1002
BmrFI CCNGG 1 cut(s) 396
BmsI GCATC 1 cut(s) 967
BoxI GACNNNNGTC 1 cut(s) 655
BpiI GAAGAC 1 cut(s) 891
BpuEI CTTGAG 1 cut(s) 90
Bsa29I ATCGAT 1 cut(s) 644
BsaAI YACGTR 3 cut(s) 178, 545, 725
BsaHI GRCGYC 2 cut(s) 404, 918
BsaJI CCNNGG 2 cut(s) 923, 995
Bse118I RCCGGY 2 cut(s) 325, 634
Bse1I ACTGG 2 cut(s) 567, 905
Bse21I CCTNAGG 1 cut(s) 385
Bse3DI GCAATG 1 cut(s) 835
BseBI CCWGG 1 cut(s) 396
BseCI ATCGAT 1 cut(s) 644
BseDI CCNNGG 2 cut(s) 923, 995
BseGI GGATG 4 cut(s) 418, 829, 898, 909
BseMI GCAATG 1 cut(s) 835
BseMII CTCAG 1 cut(s) 366
BseNI ACTGG 2 cut(s) 567, 905
BsePI GCGCGC 1 cut(s) 119
BseX3I CGGCCG 1 cut(s) 327
BseXI GCAGC 5 cut(s) 480, 561, 592, 614, 690
BseYI CCCAGC 1 cut(s) 623
BsgI GTGCAG 1 cut(s) 287
Bsh1236I CGCG 1 cut(s) 121
Bsh1285I CGRYCG 1 cut(s) 330
BshFI GGCC 5 cut(s) 182, 209, 325, 329, 631
BshVI ATCGAT 1 cut(s) 644
BsiEI CGRYCG 1 cut(s) 330
BsiHKAI GWGCWC 2 cut(s) 161, 715
BsiSI CCGG 3 cut(s) 326, 330, 635
BslFI GGGAC 1 cut(s) 905
BsmAI GTCTC 3 cut(s) 643, 891, 909
BsmBI CGTCTC 1 cut(s) 909
BsmFI GGGAC 1 cut(s) 905
BsnI GGCC 5 cut(s) 182, 209, 325, 329, 631
Bsp1286I GDGCHC 3 cut(s) 161, 715, 1003
Bsp1407I TGTACA 1 cut(s) 744
Bsp143I GATC 3 cut(s) 502, 641, 862
BspACI CCGC 7 cut(s) 148, 163, 255, 531, 600, 632, 807
BspANI GGCC 5 cut(s) 182, 209, 325, 329, 631
BspCNI CTCAG 1 cut(s) 367
BspDI ATCGAT 1 cut(s) 644
BspFNI CGCG 1 cut(s) 121
BspLI GGNNCC 1 cut(s) 1002
BspMAI CTGCAG 2 cut(s) 519, 850
BspMI ACCTGC 2 cut(s) 309, 853
BspPI GGATC 1 cut(s) 870
BsrDI GCAATG 1 cut(s) 835
BsrFI RCCGGY 2 cut(s) 325, 634
BsrGI TGTACA 1 cut(s) 744
BsrI ACTGG 2 cut(s) 567, 905
BssAI RCCGGY 2 cut(s) 325, 634
BssECI CCNNGG 2 cut(s) 923, 995
BssHII GCGCGC 1 cut(s) 119
BssMI GATC 3 cut(s) 502, 641, 862
BssNI GRCGYC 2 cut(s) 404, 918
BssSI CACGAG 3 cut(s) 203, 230, 423
BssT1I CCWWGG 1 cut(s) 923
Bst2BI CACGAG 3 cut(s) 203, 230, 423
Bst2UI CCWGG 1 cut(s) 396
Bst4CI ACNGT 4 cut(s) 20, 128, 318, 499
BstACI GRCGYC 2 cut(s) 404, 918
BstAPI GCANNNNNTGC 1 cut(s) 156
BstAUI TGTACA 1 cut(s) 744
BstBAI YACGTR 3 cut(s) 178, 545, 725
BstC8I GCNNGC 8 cut(s) 121, 152, 323, 327, 515, 535, 539, 950
BstDEI CTNAG 3 cut(s) 375, 385, 775
BstEII GGTNACC 1 cut(s) 390
BstF5I GGATG 4 cut(s) 418, 829, 898, 909
BstFNI CGCG 1 cut(s) 121
BstHHI GCGC 3 cut(s) 121, 123, 704
BstKTI GATC 3 cut(s) 505, 644, 865
BstMAI GTCTC 3 cut(s) 643, 891, 909
BstMBI GATC 3 cut(s) 502, 641, 862
BstMCI CGRYCG 1 cut(s) 330
BstMWI GCNNNNNNNGC 6 cut(s) 156, 605, 611, 620, 710, 998
BstNI CCWGG 1 cut(s) 396
BstNSI RCATGY 1 cut(s) 143
BstPAI GACNNNNGTC 1 cut(s) 655
BstPI GGTNACC 1 cut(s) 390
BstSCI CCNGG 1 cut(s) 394
BstSFI CTRYAG 2 cut(s) 515, 846
BstSNI TACGTA 1 cut(s) 725
BstUI CGCG 1 cut(s) 121
BstV1I GCAGC 5 cut(s) 480, 561, 592, 614, 690
BstV2I GAAGAC 1 cut(s) 891
BstZI CGGCCG 1 cut(s) 327
Bsu15I ATCGAT 1 cut(s) 644
Bsu36I CCTNAGG 1 cut(s) 385
BsuI GTATCC 1 cut(s) 487
BsuRI GGCC 5 cut(s) 182, 209, 325, 329, 631
BsuTUI ATCGAT 1 cut(s) 644
BtsCI GGATG 4 cut(s) 418, 829, 898, 909
BtsI GCAGTG 2 cut(s) 161, 855
BtsIMutI CAGTG 4 cut(s) 161, 323, 372, 855
BveI ACCTGC 2 cut(s) 309, 853
Cac8I GCNNGC 8 cut(s) 121, 152, 323, 327, 515, 535, 539, 950
CaiI CAGNNNCTG 1 cut(s) 980
CfoI GCGC 3 cut(s) 121, 123, 704
Cfr10I RCCGGY 2 cut(s) 325, 634
ClaI ATCGAT 1 cut(s) 644
CsiI ACCWGGT 1 cut(s) 394
Csp6I GTAC 4 cut(s) 82, 399, 564, 745
CviAII CATG 4 cut(s) 140, 358, 490, 945
CviQI GTAC 4 cut(s) 82, 399, 564, 745
DdeI CTNAG 3 cut(s) 375, 385, 775
DpnI GATC 3 cut(s) 504, 643, 864
DpnII GATC 3 cut(s) 502, 641, 862
EaeI YGGCCR 3 cut(s) 323, 327, 629
EagI CGGCCG 1 cut(s) 327
EciI GGCGGA 1 cut(s) 244
Ecl136II GAGCTC 1 cut(s) 713
EclXI CGGCCG 1 cut(s) 327
Eco105I TACGTA 1 cut(s) 725
Eco130I CCWWGG 1 cut(s) 923
Eco24I GRGCYC 2 cut(s) 715, 1003
Eco52I CGGCCG 1 cut(s) 327
Eco53kI GAGCTC 1 cut(s) 713
Eco72I CACGTG 2 cut(s) 178, 545
Eco81I CCTNAGG 1 cut(s) 385
Eco91I GGTNACC 1 cut(s) 390
EcoICRI GAGCTC 1 cut(s) 713
EcoO65I GGTNACC 1 cut(s) 390
EcoRII CCWGG 1 cut(s) 394
EcoT14I CCWWGG 1 cut(s) 923
EcoT38I GRGCYC 2 cut(s) 715, 1003
ErhI CCWWGG 1 cut(s) 923
Esp3I CGTCTC 1 cut(s) 909
FaeI CATG 4 cut(s) 143, 361, 493, 948
FaqI GGGAC 1 cut(s) 905
FatI CATG 4 cut(s) 139, 357, 489, 944
FauI CCCGC 1 cut(s) 170
FbaI TGATCA 1 cut(s) 502
Fnu4HI GCNGC 7 cut(s) 469, 550, 600, 603, 606, 632, 679
FokI GGATG 4 cut(s) 405, 836, 905, 916
FriOI GRGCYC 2 cut(s) 715, 1003
FseI GGCCGGCC 1 cut(s) 329
Fsp4HI GCNGC 7 cut(s) 469, 550, 600, 603, 606, 632, 679
FspBI CTAG 1 cut(s) 561
GlaI GCGC 3 cut(s) 120, 122, 703
GluI GCNGC 7 cut(s) 469, 550, 600, 603, 606, 632, 679
GsaI CCCAGC 1 cut(s) 627
HaeIII GGCC 5 cut(s) 182, 209, 325, 329, 631
HapII CCGG 3 cut(s) 326, 330, 635
HhaI GCGC 3 cut(s) 121, 123, 704
Hin1I GRCGYC 2 cut(s) 404, 918
Hin1II CATG 4 cut(s) 143, 361, 493, 948
Hin6I GCGC 3 cut(s) 119, 121, 702
HinP1I GCGC 3 cut(s) 119, 121, 702
HincII GTYRAC 2 cut(s) 145, 876
HindII GTYRAC 2 cut(s) 145, 876
HinfI GANTC 1 cut(s) 941
HpaII CCGG 3 cut(s) 326, 330, 635
HphI GGTGA 1 cut(s) 487
Hpy166II GTNNAC 3 cut(s) 145, 728, 876
Hpy188I TCNGA 5 cut(s) 409, 507, 976, 996, 1005
Hpy188III TCNNGA 3 cut(s) 448, 758, 1014
Hpy8I GTNNAC 3 cut(s) 145, 728, 876
Hpy99I CGWCG 1 cut(s) 406
HpyAV CCTTC 1 cut(s) 801
HpyCH4III ACNGT 4 cut(s) 20, 128, 318, 499
HpyCH4IV ACGT 7 cut(s) 177, 308, 404, 481, 544, 724, 918
HpyCH4V TGCA 6 cut(s) 139, 304, 517, 608, 848, 948
HpyF10VI GCNNNNNNNGC 6 cut(s) 156, 605, 611, 620, 710, 998
HpyF3I CTNAG 3 cut(s) 375, 385, 775
HpySE526I ACGT 7 cut(s) 177, 308, 404, 481, 544, 724, 918
Hsp92I GRCGYC 2 cut(s) 404, 918
Hsp92II CATG 4 cut(s) 143, 361, 493, 948
HspAI GCGC 3 cut(s) 119, 121, 702
KroI GCCGGC 1 cut(s) 325
KroNI GCCGGC 1 cut(s) 327
Ksp22I TGATCA 1 cut(s) 502
Kzo9I GATC 3 cut(s) 502, 641, 862
LmnI GCTCC 6 cut(s) 164, 434, 710, 718, 997, 1006
Lsp1109I GCAGC 5 cut(s) 480, 561, 592, 614, 690
LweI GCATC 1 cut(s) 967
MabI ACCWGGT 1 cut(s) 394
MaeI CTAG 1 cut(s) 561
MaeII ACGT 7 cut(s) 177, 308, 404, 481, 544, 724, 918
MaeIII GTNAC 1 cut(s) 390
MalI GATC 3 cut(s) 504, 643, 864
MboI GATC 3 cut(s) 502, 641, 862
MboII GAAGA 2 cut(s) 896, 940
MhlI GDGCHC 3 cut(s) 161, 715, 1003
MluCI AATT 1 cut(s) 241
MmeI TCCRAC 1 cut(s) 892
MroNI GCCGGC 1 cut(s) 325
MseI TTAA 4 cut(s) 282, 366, 797, 962
MslI CAYNNNNRTG 1 cut(s) 423
MspA1I CMGCKG 1 cut(s) 605
MspI CCGG 3 cut(s) 326, 330, 635
MspR9I CCNGG 1 cut(s) 396
MvaI CCWGG 1 cut(s) 396
MvnI CGCG 1 cut(s) 121
MwoI GCNNNNNNNGC 6 cut(s) 156, 605, 611, 620, 710, 998
NaeI GCCGGC 1 cut(s) 327
NdeII GATC 3 cut(s) 502, 641, 862
NgoMIV GCCGGC 1 cut(s) 325
NlaIII CATG 4 cut(s) 143, 361, 493, 948
NlaIV GGNNCC 1 cut(s) 1002
NspI RCATGY 1 cut(s) 143
PauI GCGCGC 1 cut(s) 119
PdiI GCCGGC 1 cut(s) 327
PfeI GAWTC 1 cut(s) 941
PkrI GCNGC 7 cut(s) 470, 551, 601, 604, 607, 633, 680
PmaCI CACGTG 2 cut(s) 178, 545
PmlI CACGTG 2 cut(s) 178, 545
Ppu21I YACGTR 3 cut(s) 178, 545, 725
PshAI GACNNNNGTC 1 cut(s) 655
Psp124BI GAGCTC 1 cut(s) 715
Psp6I CCWGG 1 cut(s) 394
PspCI CACGTG 2 cut(s) 178, 545
PspEI GGTNACC 1 cut(s) 390
PspFI CCCAGC 1 cut(s) 623
PspGI CCWGG 1 cut(s) 394
PspN4I GGNNCC 1 cut(s) 1002
PsrI GAACNNNNNNTAC 2 cut(s) 556, 588
PstI CTGCAG 2 cut(s) 519, 850
PstNI CAGNNNCTG 1 cut(s) 980
PteI GCGCGC 1 cut(s) 119
PvuII CAGCTG 1 cut(s) 605
RigI GGCCGGCC 1 cut(s) 329
RsaI GTAC 4 cut(s) 83, 400, 565, 746
RsaNI GTAC 4 cut(s) 82, 399, 564, 745
RseI CAYNNNNRTG 1 cut(s) 423
SacI GAGCTC 1 cut(s) 715
SaqAI TTAA 4 cut(s) 282, 366, 797, 962
SatI GCNGC 7 cut(s) 469, 550, 600, 603, 606, 632, 679
Sau3AI GATC 3 cut(s) 502, 641, 862
ScrFI CCNGG 1 cut(s) 396
SduI GDGCHC 3 cut(s) 161, 715, 1003
SexAI ACCWGGT 1 cut(s) 394
SfaNI GCATC 1 cut(s) 967
SfcI CTRYAG 2 cut(s) 515, 846
SmiMI CAYNNNNRTG 1 cut(s) 423
SmlI CTYRAG 1 cut(s) 105
SmoI CTYRAG 1 cut(s) 105
SnaBI TACGTA 1 cut(s) 725
Sse9I AATT 1 cut(s) 241
SsiI CCGC 7 cut(s) 148, 163, 255, 531, 600, 632, 807
SspI AATATT 1 cut(s) 94
SspMI CTAG 1 cut(s) 561
SstI GAGCTC 1 cut(s) 715
StyD4I CCNGG 1 cut(s) 394
StyI CCWWGG 1 cut(s) 923
TaaI ACNGT 4 cut(s) 20, 128, 318, 499
TaiI ACGT 7 cut(s) 180, 311, 407, 484, 547, 727, 921
TaqI TCGA 3 cut(s) 644, 732, 819
TasI AATT 1 cut(s) 241
TatI WGTACW 2 cut(s) 81, 744
TauI GCSGC 2 cut(s) 602, 634
TfiI GAWTC 1 cut(s) 941
Tru1I TTAA 4 cut(s) 282, 366, 797, 962
Tru9I TTAA 4 cut(s) 282, 366, 797, 962
TscAI CASTG 4 cut(s) 161, 323, 379, 855
TseI GCWGC 5 cut(s) 468, 549, 602, 605, 678
TspDTI ATGAA 6 cut(s) 17, 366, 478, 854, 897, 980
TspGWI ACGGA 1 cut(s) 577
TspRI CASTG 4 cut(s) 161, 323, 379, 855
XapI RAATTY 1 cut(s) 241
XceI RCATGY 1 cut(s) 143
XcmI CCANNNNNNNNNTGG 1 cut(s) 175
XspI CTAG 1 cut(s) 561
ZraI GACGTC 2 cut(s) 405, 919
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.