pycom16g04350

nuclear matrix constituent protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0001591
Physical Location & Seq
Forward (+)
2736083 .. 2736467
385 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g04350.2

Sequence Viewer

Length: 276 bp
ATGCAGGTCACCACATTGAAAAGTGTGGAATTCTCACAGGAGCGGGTTGTGAGGTTTAGAACACCAAAAGGAACCGTTGATGACAATGCTGAAGCTGATGCAGCAAAATCAGTTGAAAATACAGAGTTGAGTGCAGAAGATAATGGTACACCGGAATCTGGTGGCGGCAACAATACAAGTGGCGAGAGCGACGATGATTATGATGACGAGGACGTTGAAGAGCGTCCGGGCGAGAAGTCAATAGGAAAGAAGATCTGGACTTTCTTGACAACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

92

Amino Acids

9.93

Weight (kDa)

4.2

Isoelectric Point (pI)

20.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 43
AciI CCGC 2 cut(s) 43, 165
AcsI RAATTY 1 cut(s) 29
AcuI CTGAAG 1 cut(s) 111
AfaI GTAC 1 cut(s) 148
AfiI CCNNNNNNNGG 1 cut(s) 158
AgsI TTSAA 3 cut(s) 19, 116, 218
AluBI AGCT 1 cut(s) 95
AluI AGCT 1 cut(s) 95
ApeKI GCWGC 1 cut(s) 101
ApoI RAATTY 1 cut(s) 29
AsuC2I CCSGG 1 cut(s) 228
BbvI GCAGC 1 cut(s) 113
BcnI CCSGG 1 cut(s) 228
BglII AGATCT 1 cut(s) 252
BisI GCNGC 2 cut(s) 102, 166
BlsI GCNGC 2 cut(s) 103, 167
Bme1390I CCNGG 1 cut(s) 228
BmiI GGNNCC 1 cut(s) 73
BmrFI CCNGG 1 cut(s) 228
BmsI GCATC 1 cut(s) 88
BpuMI CCSGG 1 cut(s) 228
BsaWI WCCGGW 1 cut(s) 151
Bsc4I CCNNNNNNNGG 1 cut(s) 158
BseLI CCNNNNNNNGG 1 cut(s) 158
BseXI GCAGC 1 cut(s) 113
BsgI GTGCAG 1 cut(s) 153
BsiSI CCGG 2 cut(s) 152, 227
BslI CCNNNNNNNGG 1 cut(s) 158
Bsp143I GATC 1 cut(s) 252
BspACI CCGC 2 cut(s) 43, 165
BspLI GGNNCC 1 cut(s) 73
BspQI GCTCTTC 1 cut(s) 213
BsrBI CCGCTC 1 cut(s) 43
BssMI GATC 1 cut(s) 252
Bst4CI ACNGT 1 cut(s) 76
Bst6I CTCTTC 1 cut(s) 213
BstEII GGTNACC 1 cut(s) 7
BstKTI GATC 1 cut(s) 255
BstMBI GATC 1 cut(s) 252
BstMWI GCNNNNNNNGC 1 cut(s) 101
BstPI GGTNACC 1 cut(s) 7
BstSCI CCNGG 1 cut(s) 226
BstV1I GCAGC 1 cut(s) 113
BstX2I RGATCY 1 cut(s) 252
BstYI RGATCY 1 cut(s) 252
CseI GACGC 1 cut(s) 212
Csp6I GTAC 1 cut(s) 147
CspCI CAANNNNNGTGG 2 cut(s) 160, 195
CviJI RGCY 1 cut(s) 95
CviKI_1 RGCY 1 cut(s) 95
CviQI GTAC 1 cut(s) 147
DpnI GATC 1 cut(s) 254
DpnII GATC 1 cut(s) 252
Eam1104I CTCTTC 1 cut(s) 213
EarI CTCTTC 1 cut(s) 213
Eco57I CTGAAG 1 cut(s) 111
Eco91I GGTNACC 1 cut(s) 7
EcoO65I GGTNACC 1 cut(s) 7
EcoRI GAATTC 1 cut(s) 29
FaiI YATR 1 cut(s) 201
FauI CCCGC 1 cut(s) 36
Fnu4HI GCNGC 2 cut(s) 102, 166
Fsp4HI GCNGC 2 cut(s) 102, 166
GluI GCNGC 2 cut(s) 102, 166
HapII CCGG 2 cut(s) 152, 227
HgaI GACGC 1 cut(s) 212
HinfI GANTC 1 cut(s) 155
HpaII CCGG 2 cut(s) 152, 227
Hpy166II GTNNAC 1 cut(s) 149
Hpy188III TCNNGA 2 cut(s) 256, 265
Hpy8I GTNNAC 1 cut(s) 149
Hpy99I CGWCG 1 cut(s) 194
HpyCH4III ACNGT 1 cut(s) 76
HpyCH4IV ACGT 2 cut(s) 213, 272
HpyCH4V TGCA 3 cut(s) 4, 101, 134
HpyF10VI GCNNNNNNNGC 1 cut(s) 101
HpySE526I ACGT 2 cut(s) 213, 272
Kzo9I GATC 1 cut(s) 252
LguI GCTCTTC 1 cut(s) 213
LmnI GCTCC 1 cut(s) 40
LpnPI CCDG 5 cut(s) 23, 144, 165, 240, 241
Lsp1109I GCAGC 1 cut(s) 113
LweI GCATC 1 cut(s) 88
MaeII ACGT 2 cut(s) 213, 272
MaeIII GTNAC 1 cut(s) 7
MalI GATC 1 cut(s) 254
MbiI CCGCTC 1 cut(s) 43
MboI GATC 1 cut(s) 252
MboII GAAGA 3 cut(s) 149, 230, 262
MflI RGATCY 1 cut(s) 252
MluCI AATT 1 cut(s) 29
MnlI CCTC 2 cut(s) 45, 202
MspI CCGG 2 cut(s) 152, 227
MspR9I CCNGG 1 cut(s) 228
MwoI GCNNNNNNNGC 1 cut(s) 101
NciI CCSGG 1 cut(s) 228
NdeII GATC 1 cut(s) 252
NlaIV GGNNCC 1 cut(s) 73
NmuCI GTSAC 1 cut(s) 7
PciSI GCTCTTC 1 cut(s) 213
PfeI GAWTC 1 cut(s) 155
PkrI GCNGC 2 cut(s) 103, 167
PspEI GGTNACC 1 cut(s) 7
PspN4I GGNNCC 1 cut(s) 73
PsuI RGATCY 1 cut(s) 252
RsaI GTAC 1 cut(s) 148
RsaNI GTAC 1 cut(s) 147
SapI GCTCTTC 1 cut(s) 213
SatI GCNGC 2 cut(s) 102, 166
Sau3AI GATC 1 cut(s) 252
ScrFI CCNGG 1 cut(s) 228
SetI ASST 5 cut(s) 9, 56, 97, 216, 275
SfaNI GCATC 1 cut(s) 88
Sse9I AATT 1 cut(s) 29
SsiI CCGC 2 cut(s) 43, 165
StyD4I CCNGG 1 cut(s) 226
TaaI ACNGT 1 cut(s) 76
TaiI ACGT 2 cut(s) 216, 275
TasI AATT 1 cut(s) 29
TauI GCSGC 1 cut(s) 168
TfiI GAWTC 1 cut(s) 155
TseFI GTSAC 1 cut(s) 7
TseI GCWGC 1 cut(s) 101
Tsp45I GTSAC 1 cut(s) 7
XapI RAATTY 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.