Rroxscaffold_3G00233250

nuclear matrix constituent protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001591
Physical Location & Seq
Reverse (-)
19186403 .. 19191897
5495 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00233250.1

Sequence Viewer

Length: 3372 bp
ATGTTCACGCCGCTGAGTAAGGCGCCGGCGACGCCGAGGACCACCGACAAGGGCAAAGCCGTGGCGTACGTCGACGGTCCTCCGCCGCCACTTGGTTCTCTGAGCGAAATCAGAAGCGGCGGCGGCGGGGCCAAAACGTCGCCGGATTTGGACAATGCCGATTGGAGGCGGTTTAGGGAGGTTGGGCTGCTAGATGAGGCGGCCATGGAGCGCCGAGACCGCCAAGAACTCGCCAATAAGGTCGCTAGGCTTGAGTCTGAGCTTCATGATTACCAACATAGCATGGGGCTTCTCCTCATTGAGAAGAAAGAATGGGCTTTGGAGCATGAGGAGTTGAGCCAAGCATTGGCTGAAACCCATGAGATTCTACACCGGGAGCAGAGGGCTCATCTGATTGCAATTTCGGAACTCGAAAGGCGAGAGGAGAATTTGAGGAAGATATTGGTGGAGGAAAAGAGGTCTGCAGCTGAGCTCGAAAAGTCATTGCGTGAAATGCACGAGGAGTATACCCGGATAAAGCATGCATCTGAGGCGAAGGTGGCTGATGCGAATGCGTTGATTGTTAGTGTTGAAGATAAATCTCTAGTGACGGATGAGAAGTTTCTTGCTGCTGAAGCCAAGCTTGCTGAGGCCAACAAAAAGAGTCTAGAGGTGGAGAGGAGGTTGCAAGAGGTGGAGACACGTGAGAGTGTGCTTCGGAGGGAGCAAGCGTCGTTAAGTACAGAGAGAGAGGCACACAAGACAACTTTTTACAGACAAAGAGAAGACTTGAACGAGTGGGAGAAGAAGTTAAAAGAAGGGGAAGAGAGGCTAATCAATCTTCGTAAAATTTTGAACGAGAAAGAGGAAAAAACAAATGAAAACGATATGATTCTTAAGCAAAAAGAGAAAGACCTTTATGAGGCTGAAAGGAAGATTGAGTCTTCAAACTATCTGTTGAAAGAGAAGGAAGGTGATGTGAATAAACGCCTAGCTGACCTAGTCTCAAAGGAAAAGGAGGTTGATTCAGCTTCATACATCTTAGAGAGGAAGGAGAAAGAATTACATTCATTGGAGGAAAAACTAAATTCAAGGGAAAATGTGGAGATTCAAGAGCATCTTGACCAGCACAGGGCCATTCTTGATAGAAAAATGCAGGCTTTTGAGTTGGGAATTGAGGAAAGGAGGAAATCTTATGAGAAGGAGCAGAGTAGCAAGATAGAAGCGGTGGAACAAAAGGAACTTGAAATCAGTCATAAGGAAGAAATGTTGAAGAAGCAAGAGAAAGCGTTGGATGAGAAATCAGAGAGGCTGAAGGAGAAAAATAAGGAAGTTGAAACAAATTTGAAAAATTTGAAGGAGAAAGAGAAGACCTTCAAAGCTGATGAGAAAAAGTTAGAGCTGGAAAGGCAGCAAATACTTACTGATATAGAGCACCTTCAGAATCTTAAAGATGAAATTCAGAAAATAAAAGACGAAAATGCTCAACTGGAGCAGCAAATTCGTGAAGGCAGAGAGAAGCAGGGAATTACCGAGAAAGAGAGATCTGAAGCAGGAGAGGGAAAAGTTCGAAAAGAGTGGGAAGATTTGGATGAGAGAAGAGCTAAAGTTGATGTAGAACTTAGAAAAGTTGTTGAAGAGAAAGAAAAATTGGAAAGATTACAATGTATTGAAGCAGAGAGGTTGAAAGTAGAGAAACAAGCAGTGCAAGATTACAGACAGAGGGAGATGGAAAATCTCAAGCAGGAGAAAGAATCATTTGCAGCTAAGATGAGTAATGAGCAGGTAGCCTTATCTGAAAAGGCCCAATCTGAGCATGCTCAAATGGTCCAAGATTTTGAGTCACGGAGAAGAGATCTTGAGATTGGTATGCAGAATAGGCAGGATAAGATGGTAAAACAACTGCAGGAAAGGGAGAGAGCATTTGAGGAGGAGAAGGATAGAGAACATACTAATATTAATTTCTTGAAGGGAGTTGCTGATAAGCAAAGAGAAGAGCTATTATCTGAAAGGAATACGAATGAAAAGGAAAGGGAAGCGCTTGCACTGCAGAAAAAGGAGCTGGAAGCGAACCAACTTGAAATGCGAGAAGACATAGATCAGCTTGACAAGCTTAGCAAGAAGATCAAGTGCCAGAGGGAACAGCTGATTGAGGAAAGAGGTCGCTTTCTTGCCTTTGTTGAGAAGGTTAAGAGTTGCAAGGACTGTGGAGAAATAGCAAGGGAATTTGTTCTCTCTGATCTCCAAGTACCTGGGATGTACCATGCTGAGGCTGTTCCTGATTCTGAACATAAAGAGTCAGGATGGGGGAAAAAACTGCAACAGAAATGCAAATTAGTGGTTTCCAAAGTAACATCAAATAAAAAACATGCTGTATCTACTGAGTTACCCCCACTATCGGCTATGCAGAAAGGAAAGGAACCCAACTTGCTTGCTGATGAGGAAGCAAGAGGTGCTAGCAGTCATGAAGAGCCTCAGCCATCTGTTAGGAGATGTAATGACTCGGCCAATGCTCAAGCAGTCGTAACTGATAACAACTCTAAGAAGGTGGATGATGGGTATGCTCCATCAATTGACGATCACAGTTTCGTTGACAGCCAGGAGCAAGACATTCCAGAAGATTCTGAGCAATCAGAGCTGAAAAGTGGCCGACGAAAACCAGCTAGGGGACGCAAGTCTAGATTGTCTAGAACACACTCAGTGAAGGAAGCTGTTGAAGACGCAAAGAAATTTTTAGGAGAAACTCCAGAAGCATCAAATGCAAGTTTGCTTAATGAGAGTAGTTACATAAATGAAGGTGATTCCAGTTTTGCGAGCATCGGTCGGAAACGAGCCCGCCCTCAGAGCTCCAGGATAGAAAGCGAGCAAGATGATTGTGATAGCGAAGGACGTTCTGGTAGTGTCACAGCTGGTGGGCGTAGGAAGAGGCGCGAACCCGTTGCTTCAGCAGTGCAAACCCCTGGAGAACAGCGATATAATCTCAGACACCGCAAGACTGCAGGCACATTGGCAGCAACATCAGCAGCGCCTCACCCGAAAAATAGAAGGAAGGAAGAATCAAATCCTGAATCTGTTGGTGTAGACCTGATTCAGGTCACAACATTAAAAACTGTGGAGTCAACAGAGGAGCGAGTTGTGAGGTTTGCAACACCAGAACCACAAGACACTGTTAATGGCAATGCTGATGCTGACGCTGACGCTGATGCAACGAAGTTGGTTGAAGAGACAGAGTTGAGTACGGAAGTGAATGGCACAGACTCTAGTGGTGGCAACAGCACAGGCGGTGAGATTGGCAACAGCACAGGTGATGAGAGTGGCGATGACTATGATGATGAGGATCATCCTGGTCAAGCATCAATAGGGAAGAAGATCTGGACCTTCTTCAGCACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1123

Amino Acids

128.62

Weight (kDa)

5.39

Isoelectric Point (pI)

52.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1753
AccB1I GGYRCC 1 cut(s) 22
AccB7I CCANNNNNTGG 1 cut(s) 346
AccI GTMKAC 3 cut(s) 72, 506, 3060
AccII CGCG 1 cut(s) 2910
AclWI GGATC 1 cut(s) 3324
AcoI YGGCCR 3 cut(s) 201, 2484, 2626
AcsI RAATTY 9 cut(s) 427, 828, 1066, 1321, 1330, 1437, 1479, 2204, 2708
AcuI CTGAAG 6 cut(s) 633, 1313, 1403, 1548, 2907, 3346
AcvI CACGTG 1 cut(s) 683
AcyI GRCGYC 2 cut(s) 23, 32
AdeI CACNNNGTG 1 cut(s) 2680
AfaI GTAC 5 cut(s) 68, 721, 2229, 2240, 3217
AfeI AGCGCT 1 cut(s) 2019
AfiI CCNNNNNNNGG 9 cut(s) 92, 165, 346, 901, 1111, 2248, 2377, 2645, 3070
AflII CTTAAG 1 cut(s) 875
AflIII ACRYGT 1 cut(s) 680
AjnI CCWGG 5 cut(s) 2230, 2577, 2828, 2938, 3322
AjuI GAANNNNNNNTTGG 2 cut(s) 1613, 1645
Alw21I GWGCWC 3 cut(s) 474, 1416, 2828
Alw26I GTCTC 4 cut(s) 210, 671, 988, 3197
AlwI GGATC 1 cut(s) 3324
Aor51HI AGCGCT 1 cut(s) 2019
AoxI GGCC 7 cut(s) 129, 201, 630, 1113, 1782, 2484, 2626
ApeKI GCWGC 8 cut(s) 187, 464, 608, 1390, 1474, 1742, 2990, 3002
ApoI RAATTY 9 cut(s) 427, 828, 1066, 1321, 1330, 1437, 1479, 2204, 2708
AseI ATTAAT 1 cut(s) 1938
Asp700I GAANNNNTTC 2 cut(s) 1352, 2208
AspLEI GCGC 5 cut(s) 25, 213, 2020, 2910, 3007
AspS9I GGNCC 7 cut(s) 39, 77, 129, 1113, 1783, 1807, 3354
AsuC2I CCSGG 2 cut(s) 374, 511
AsuHPI GGTGA 5 cut(s) 965, 2789, 3002, 3275, 3296
AsuII TTCGAA 1 cut(s) 1549
AsuNHI GCTAGC 1 cut(s) 2435
AvaII GGWCC 4 cut(s) 39, 77, 1807, 3354
BanI GGYRCC 1 cut(s) 22
BanII GRGCYC 4 cut(s) 388, 474, 2815, 2828
BauI CACGAG 1 cut(s) 497
BbrPI CACGTG 1 cut(s) 683
BbsI GAAGAC 5 cut(s) 771, 915, 1355, 2076, 2703
Bbv12I GWGCWC 3 cut(s) 474, 1416, 2828
BbvCI CCTCAGC 3 cut(s) 627, 2247, 2454
BbvI GCAGC 8 cut(s) 174, 476, 595, 1402, 1486, 1754, 3002, 3014
BccI CCATC 6 cut(s) 1702, 1864, 2277, 2467, 2528, 2554
BceAI ACGGC 1 cut(s) 44
BcgI CGANNNNNNTGC 4 cut(s) 1463, 1497, 2900, 2934
BciT130I CCWGG 5 cut(s) 2232, 2579, 2830, 2940, 3324
BcnI CCSGG 2 cut(s) 374, 511
BcoDI GTCTC 4 cut(s) 210, 671, 988, 3197
BfmI CTRYAG 4 cut(s) 462, 1883, 2027, 2976
BfoI RGCGCY 4 cut(s) 26, 214, 2021, 3008
BfrI CTTAAG 1 cut(s) 875
BfuAI ACCTGC 1 cut(s) 1753
BglII AGATCT 3 cut(s) 1523, 1834, 3348
BlpI GCTNAGC 2 cut(s) 468, 2093
Bme1390I CCNGG 7 cut(s) 374, 511, 2232, 2579, 2830, 2940, 3324
Bme18I GGWCC 4 cut(s) 39, 77, 1807, 3354
BmgT120I GGNCC 7 cut(s) 39, 77, 129, 1113, 1783, 1807, 3354
BmiI GGNNCC 3 cut(s) 24, 130, 2400
BmrFI CCNGG 7 cut(s) 374, 511, 2232, 2579, 2830, 2940, 3324
BmsI GCATC 8 cut(s) 533, 535, 1105, 2741, 2805, 3154, 3172, 3341
BmtI GCTAGC 1 cut(s) 2439
BoxI GACNNNNGTC 1 cut(s) 2653
BpiI GAAGAC 5 cut(s) 771, 915, 1355, 2076, 2703
BpmI CTGGAG 4 cut(s) 1490, 2709, 2812, 2961
Bpu10I CCTNAGC 3 cut(s) 627, 2247, 2454
Bpu1102I GCTNAGC 2 cut(s) 468, 2093
Bpu14I TTCGAA 1 cut(s) 1549
BpuEI CTTGAG 4 cut(s) 272, 1703, 1859, 2478
BpuMI CCSGG 2 cut(s) 374, 511
BsaAI YACGTR 1 cut(s) 683
BsaBI GATNNNNATC 1 cut(s) 3315
BsaHI GRCGYC 2 cut(s) 23, 32
BsaI GGTCTC 1 cut(s) 210
BsaJI CCNNGG 5 cut(s) 35, 60, 204, 2231, 2938
BsaXI ACNNNNNCTCC 6 cut(s) 647, 677, 695, 725, 773, 803
Bsc4I CCNNNNNNNGG 9 cut(s) 92, 165, 346, 901, 1111, 2248, 2377, 2645, 3070
Bse118I RCCGGY 1 cut(s) 25
Bse1I ACTGG 2 cut(s) 1473, 2784
Bse3DI GCAATG 2 cut(s) 482, 3163
Bse8I GATNNNNATC 1 cut(s) 3315
BseBI CCWGG 5 cut(s) 2232, 2579, 2830, 2940, 3324
BseDI CCNNGG 5 cut(s) 35, 60, 204, 2231, 2938
BseGI GGATG 7 cut(s) 598, 1279, 1576, 2241, 2288, 2536, 3319
BseJI GATNNNNATC 1 cut(s) 3315
BseLI CCNNNNNNNGG 9 cut(s) 92, 165, 346, 901, 1111, 2248, 2377, 2645, 3070
BseMI GCAATG 2 cut(s) 482, 3163
BseNI ACTGG 2 cut(s) 1473, 2784
BseRI GAGGAG 8 cut(s) 284, 344, 437, 515, 673, 1922, 1925, 3119
BseXI GCAGC 8 cut(s) 174, 476, 595, 1402, 1486, 1754, 3002, 3014
Bsh1236I CGCG 1 cut(s) 2910
Bsh1285I CGRYCG 1 cut(s) 2803
BshFI GGCC 7 cut(s) 131, 203, 632, 1115, 1784, 2486, 2628
BshNI GGYRCC 1 cut(s) 22
BsiEI CGRYCG 1 cut(s) 2803
BsiHKAI GWGCWC 3 cut(s) 474, 1416, 2828
BsiSI CCGG 4 cut(s) 26, 143, 373, 511
BsiWI CGTACG 1 cut(s) 66
BslFI GGGAC 1 cut(s) 2661
BslI CCNNNNNNNGG 9 cut(s) 92, 165, 346, 901, 1111, 2248, 2377, 2645, 3070
BsmAI GTCTC 4 cut(s) 210, 671, 988, 3197
BsmFI GGGAC 1 cut(s) 2661
BsmI GAATGC 1 cut(s) 556
BsnI GGCC 7 cut(s) 131, 203, 632, 1115, 1784, 2486, 2628
Bso31I GGTCTC 1 cut(s) 210
Bsp119I TTCGAA 1 cut(s) 1549
Bsp1286I GDGCHC 5 cut(s) 388, 474, 1416, 2815, 2828
Bsp143I GATC 8 cut(s) 1523, 1834, 2077, 2103, 2218, 2557, 3316, 3348
Bsp1720I GCTNAGC 2 cut(s) 468, 2093
Bsp19I CCATGG 1 cut(s) 204
BspANI GGCC 7 cut(s) 131, 203, 632, 1115, 1784, 2486, 2628
BspFNI CGCG 1 cut(s) 2910
BspHI TCATGA 2 cut(s) 265, 2443
BspLI GGNNCC 3 cut(s) 24, 130, 2400
BspMAI CTGCAG 4 cut(s) 466, 1887, 2031, 2980
BspMI ACCTGC 1 cut(s) 1753
BspOI GCTAGC 1 cut(s) 2439
BspPI GGATC 1 cut(s) 3324
BspQI GCTCTTC 3 cut(s) 1573, 1968, 2442
BspT104I TTCGAA 1 cut(s) 1549
BspT107I GGYRCC 1 cut(s) 22
BspTI CTTAAG 1 cut(s) 875
BspTNI GGTCTC 1 cut(s) 210
BsrDI GCAATG 2 cut(s) 482, 3163
BsrFI RCCGGY 1 cut(s) 25
BsrI ACTGG 2 cut(s) 1473, 2784
BssAI RCCGGY 1 cut(s) 25
BssECI CCNNGG 5 cut(s) 35, 60, 204, 2231, 2938
BssMI GATC 8 cut(s) 1523, 1834, 2077, 2103, 2218, 2557, 3316, 3348
BssNAI GTATAC 1 cut(s) 507
BssNI GRCGYC 2 cut(s) 23, 32
BssSI CACGAG 1 cut(s) 497
BssT1I CCWWGG 1 cut(s) 204
Bst1107I GTATAC 1 cut(s) 507
Bst2BI CACGAG 1 cut(s) 497
Bst2UI CCWGG 5 cut(s) 2232, 2579, 2830, 2940, 3324
Bst4CI ACNGT 5 cut(s) 77, 2186, 2564, 3091, 3148
Bst6I CTCTTC 8 cut(s) 798, 1573, 1611, 1825, 1968, 2442, 2897, 3195
BstACI GRCGYC 2 cut(s) 23, 32
BstAFI CTTAAG 1 cut(s) 875
BstAPI GCANNNNNTGC 2 cut(s) 2432, 2738
BstBAI YACGTR 1 cut(s) 683
BstBI TTCGAA 1 cut(s) 1549
BstDSI CCRYGG 2 cut(s) 60, 204
BstENI CCTNNNNNAGG 2 cut(s) 899, 3068
BstF5I GGATG 7 cut(s) 598, 1279, 1576, 2241, 2288, 2536, 3319
BstFNI CGCG 1 cut(s) 2910
BstH2I RGCGCY 4 cut(s) 26, 214, 2021, 3008
BstHHI GCGC 5 cut(s) 25, 213, 2020, 2910, 3007
BstKTI GATC 8 cut(s) 1526, 1837, 2080, 2106, 2221, 2560, 3319, 3351
BstMAI GTCTC 4 cut(s) 210, 671, 988, 3197
BstMBI GATC 8 cut(s) 1523, 1834, 2077, 2103, 2218, 2557, 3316, 3348
BstMCI CGRYCG 1 cut(s) 2803
BstNI CCWGG 5 cut(s) 2232, 2579, 2830, 2940, 3324
BstNSI RCATGY 3 cut(s) 524, 1799, 2351
BstPAI GACNNNNGTC 1 cut(s) 2653
BstSCI CCNGG 7 cut(s) 372, 509, 2230, 2577, 2828, 2938, 3322
BstSFI CTRYAG 4 cut(s) 462, 1883, 2027, 2976
BstUI CGCG 1 cut(s) 2910
BstV1I GCAGC 8 cut(s) 174, 476, 595, 1402, 1486, 1754, 3002, 3014
BstV2I GAAGAC 5 cut(s) 771, 915, 1355, 2076, 2703
BstX2I RGATCY 3 cut(s) 1523, 1834, 3348
BstXI CCANNNNNNTGG 1 cut(s) 2231
BstYI RGATCY 3 cut(s) 1523, 1834, 3348
BstZ17I GTATAC 1 cut(s) 507
BsuRI GGCC 7 cut(s) 131, 203, 632, 1115, 1784, 2486, 2628
BtgI CCRYGG 2 cut(s) 60, 204
BtgZI GCGATG 1 cut(s) 3312
BtsCI GGATG 7 cut(s) 598, 1279, 1576, 2241, 2288, 2536, 3319
BtsI GCAGTG 3 cut(s) 1689, 2024, 2934
BtsIMutI CAGTG 5 cut(s) 1689, 2024, 2685, 2934, 3144
BveI ACCTGC 1 cut(s) 1753
CciI TCATGA 2 cut(s) 265, 2443
CfoI GCGC 5 cut(s) 25, 213, 2020, 2910, 3007
Cfr10I RCCGGY 1 cut(s) 25
Cfr13I GGNCC 7 cut(s) 39, 77, 129, 1113, 1783, 1807, 3354
CseI GACGC 6 cut(s) 40, 699, 2658, 2708, 3179, 3185
Csp6I GTAC 5 cut(s) 67, 720, 2228, 2239, 3216
CspCI CAANNNNNGTGG 2 cut(s) 2167, 2202
CviQI GTAC 5 cut(s) 67, 720, 2228, 2239, 3216
DinI GGCGCC 1 cut(s) 24
DpnI GATC 8 cut(s) 1525, 1836, 2079, 2105, 2220, 2559, 3318, 3350
DpnII GATC 8 cut(s) 1523, 1834, 2077, 2103, 2218, 2557, 3316, 3348
DraIII CACNNNGTG 1 cut(s) 2680
EaeI YGGCCR 3 cut(s) 201, 2484, 2626
Eam1104I CTCTTC 8 cut(s) 798, 1573, 1611, 1825, 1968, 2442, 2897, 3195
EarI CTCTTC 8 cut(s) 798, 1573, 1611, 1825, 1968, 2442, 2897, 3195
EciI GGCGGA 1 cut(s) 72
Ecl136II GAGCTC 2 cut(s) 472, 2826
Eco130I CCWWGG 1 cut(s) 204
Eco24I GRGCYC 4 cut(s) 388, 474, 2815, 2828
Eco31I GGTCTC 1 cut(s) 210
Eco47I GGWCC 4 cut(s) 39, 77, 1807, 3354
Eco47III AGCGCT 1 cut(s) 2019
Eco53kI GAGCTC 2 cut(s) 472, 2826
Eco57I CTGAAG 6 cut(s) 633, 1313, 1403, 1548, 2907, 3346
Eco72I CACGTG 1 cut(s) 683
EcoICRI GAGCTC 2 cut(s) 472, 2826
EcoNI CCTNNNNNAGG 2 cut(s) 899, 3068
EcoRII CCWGG 5 cut(s) 2230, 2577, 2828, 2938, 3322
EcoT14I CCWWGG 1 cut(s) 204
EcoT22I ATGCAT 1 cut(s) 526
EcoT38I GRGCYC 4 cut(s) 388, 474, 2815, 2828
EgeI GGCGCC 1 cut(s) 24
EheI GGCGCC 1 cut(s) 24
ErhI CCWWGG 1 cut(s) 204
FalI AAGNNNNNCTT 4 cut(s) 606, 638, 1083, 1115
FaqI GGGAC 1 cut(s) 2661
FauI CCCGC 2 cut(s) 119, 2822
FblI GTMKAC 3 cut(s) 72, 506, 3060
FokI GGATG 7 cut(s) 605, 1286, 1583, 2248, 2295, 2543, 3306
FriOI GRGCYC 4 cut(s) 388, 474, 2815, 2828
GlaI GCGC 5 cut(s) 24, 212, 2019, 2909, 3006
GsuI CTGGAG 4 cut(s) 1490, 2709, 2812, 2961
HaeII RGCGCY 4 cut(s) 26, 214, 2021, 3008
HaeIII GGCC 7 cut(s) 131, 203, 632, 1115, 1784, 2486, 2628
HapII CCGG 4 cut(s) 26, 143, 373, 511
HgaI GACGC 6 cut(s) 40, 699, 2658, 2708, 3179, 3185
HhaI GCGC 5 cut(s) 25, 213, 2020, 2910, 3007
Hin1I GRCGYC 2 cut(s) 23, 32
Hin6I GCGC 5 cut(s) 23, 211, 2018, 2908, 3005
HinP1I GCGC 5 cut(s) 23, 211, 2018, 2908, 3005
HincII GTYRAC 3 cut(s) 73, 2572, 3099
HindII GTYRAC 3 cut(s) 73, 2572, 3099
HindIII AAGCTT 2 cut(s) 620, 2090
HpaII CCGG 4 cut(s) 26, 143, 373, 511
HphI GGTGA 5 cut(s) 965, 2789, 3002, 3275, 3296
Hpy166II GTNNAC 6 cut(s) 6, 73, 507, 2572, 3061, 3099
Hpy8I GTNNAC 6 cut(s) 6, 73, 507, 2572, 3061, 3099
Hpy99I CGWCG 6 cut(s) 34, 74, 77, 142, 715, 2634
HpyCH4III ACNGT 5 cut(s) 77, 2186, 2564, 3091, 3148
HpyCH4IV ACGT 4 cut(s) 69, 137, 682, 2869
HpySE526I ACGT 4 cut(s) 69, 137, 682, 2869
Hsp92I GRCGYC 2 cut(s) 23, 32
HspAI GCGC 5 cut(s) 23, 211, 2018, 2908, 3005
KasI GGCGCC 1 cut(s) 22
KroI GCCGGC 1 cut(s) 25
KroNI GCCGGC 1 cut(s) 27
Kzo9I GATC 8 cut(s) 1523, 1834, 2077, 2103, 2218, 2557, 3316, 3348
LguI GCTCTTC 3 cut(s) 1573, 1968, 2442
Lsp1109I GCAGC 8 cut(s) 174, 476, 595, 1402, 1486, 1754, 3002, 3014
LweI GCATC 8 cut(s) 533, 535, 1105, 2741, 2805, 3154, 3172, 3341
MaeII ACGT 4 cut(s) 69, 137, 682, 2869
MaeIII GTNAC 8 cut(s) 586, 1821, 2329, 2364, 2503, 2762, 2881, 3073
MalI GATC 8 cut(s) 1525, 1836, 2079, 2105, 2220, 2559, 3318, 3350
MboI GATC 8 cut(s) 1523, 1834, 2077, 2103, 2218, 2557, 3316, 3348
MfeI CAATTG 1 cut(s) 2550
MflI RGATCY 3 cut(s) 1523, 1834, 3348
MhlI GDGCHC 5 cut(s) 388, 474, 1416, 2815, 2828
Mly113I GGCGCC 1 cut(s) 23
MlyI GAGTC 8 cut(s) 263, 652, 929, 1829, 2285, 2474, 3104, 3230
MmeI TCCRAC 2 cut(s) 1251, 2783
Mph1103I ATGCAT 1 cut(s) 526
MreI CGCCGGCG 1 cut(s) 25
MroNI GCCGGC 1 cut(s) 25
MroXI GAANNNNTTC 2 cut(s) 1352, 2208
MseI TTAA 9 cut(s) 716, 791, 876, 1428, 1938, 2169, 2751, 3083, 3150
MspA1I CMGCKG 4 cut(s) 13, 467, 2125, 2888
MspCI CTTAAG 1 cut(s) 875
MspI CCGG 4 cut(s) 26, 143, 373, 511
MspR9I CCNGG 7 cut(s) 374, 511, 2232, 2579, 2830, 2940, 3324
MunI CAATTG 1 cut(s) 2550
Mva1269I GAATGC 1 cut(s) 556
MvaI CCWGG 5 cut(s) 2232, 2579, 2830, 2940, 3324
MvnI CGCG 1 cut(s) 2910
NaeI GCCGGC 1 cut(s) 27
NarI GGCGCC 1 cut(s) 23
NciI CCSGG 2 cut(s) 374, 511
NcoI CCATGG 1 cut(s) 204
NdeII GATC 8 cut(s) 1523, 1834, 2077, 2103, 2218, 2557, 3316, 3348
NgoMIV GCCGGC 1 cut(s) 25
NheI GCTAGC 1 cut(s) 2435
NlaIV GGNNCC 3 cut(s) 24, 130, 2400
NmeAIII GCCGAG 3 cut(s) 60, 239, 2462
NmuCI GTSAC 4 cut(s) 586, 1821, 2881, 3073
NsiI ATGCAT 1 cut(s) 526
NspI RCATGY 3 cut(s) 524, 1799, 2351
NspV TTCGAA 1 cut(s) 1549
PaeI GCATGC 2 cut(s) 524, 1799
PagI TCATGA 2 cut(s) 265, 2443
PciSI GCTCTTC 3 cut(s) 1573, 1968, 2442
PctI GAATGC 1 cut(s) 556
PdiI GCCGGC 1 cut(s) 27
PdmI GAANNNNTTC 2 cut(s) 1352, 2208
Pfl23II CGTACG 1 cut(s) 66
PflFI GACNNNGTC 1 cut(s) 980
PflMI CCANNNNNTGG 1 cut(s) 346
PfoI TCCNGGA 1 cut(s) 2828
PleI GAGTC 8 cut(s) 262, 651, 928, 1828, 2284, 2474, 3103, 3230
PluTI GGCGCC 1 cut(s) 26
PmaCI CACGTG 1 cut(s) 683
PmlI CACGTG 1 cut(s) 683
PpsI GAGTC 8 cut(s) 262, 651, 928, 1828, 2284, 2474, 3103, 3230
Ppu21I YACGTR 1 cut(s) 683
PshAI GACNNNNGTC 1 cut(s) 2653
PshBI ATTAAT 1 cut(s) 1938
Psp124BI GAGCTC 2 cut(s) 474, 2828
Psp6I CCWGG 5 cut(s) 2230, 2577, 2828, 2938, 3322
PspCI CACGTG 1 cut(s) 683
PspGI CCWGG 5 cut(s) 2230, 2577, 2828, 2938, 3322
PspLI CGTACG 1 cut(s) 66
PspN4I GGNNCC 3 cut(s) 24, 130, 2400
PspPI GGNCC 7 cut(s) 39, 77, 129, 1113, 1783, 1807, 3354
PstI CTGCAG 4 cut(s) 466, 1887, 2031, 2980
PsuI RGATCY 3 cut(s) 1523, 1834, 3348
PsyI GACNNNGTC 1 cut(s) 980
PvuII CAGCTG 3 cut(s) 467, 2125, 2888
RsaI GTAC 5 cut(s) 68, 721, 2229, 2240, 3217
RsaNI GTAC 5 cut(s) 67, 720, 2228, 2239, 3216
SacI GAGCTC 2 cut(s) 474, 2828
SalI GTCGAC 1 cut(s) 71
SapI GCTCTTC 3 cut(s) 1573, 1968, 2442
SaqAI TTAA 9 cut(s) 716, 791, 876, 1428, 1938, 2169, 2751, 3083, 3150
Sau3AI GATC 8 cut(s) 1523, 1834, 2077, 2103, 2218, 2557, 3316, 3348
Sau96I GGNCC 7 cut(s) 39, 77, 129, 1113, 1783, 1807, 3354
SchI GAGTC 8 cut(s) 263, 652, 929, 1829, 2285, 2474, 3104, 3230
ScrFI CCNGG 7 cut(s) 374, 511, 2232, 2579, 2830, 2940, 3324
SduI GDGCHC 5 cut(s) 388, 474, 1416, 2815, 2828
SfaNI GCATC 8 cut(s) 533, 535, 1105, 2741, 2805, 3154, 3172, 3341
SfcI CTRYAG 4 cut(s) 462, 1883, 2027, 2976
SfoI GGCGCC 1 cut(s) 24
SfuI TTCGAA 1 cut(s) 1549
SgrAI CRCCGGYG 1 cut(s) 25
SgrDI CGTCGACG 1 cut(s) 71
SinI GGWCC 4 cut(s) 39, 77, 1807, 3354
SmlI CTYRAG 5 cut(s) 251, 875, 1718, 1838, 2493
SmoI CTYRAG 5 cut(s) 251, 875, 1718, 1838, 2493
SphI GCATGC 2 cut(s) 524, 1799
SspDI GGCGCC 1 cut(s) 22
SspI AATATT 1 cut(s) 1936
SstI GAGCTC 2 cut(s) 474, 2828
StyD4I CCNGG 7 cut(s) 372, 509, 2230, 2577, 2828, 2938, 3322
StyI CCWWGG 1 cut(s) 204
TaaI ACNGT 5 cut(s) 77, 2186, 2564, 3091, 3148
TaiI ACGT 4 cut(s) 72, 140, 685, 2872
TaqI TCGA 4 cut(s) 72, 411, 474, 1549
TaqII GACCGA 1 cut(s) 2789
TatI WGTACW 1 cut(s) 719
TauI GCSGC 6 cut(s) 13, 88, 120, 123, 126, 203
Tru1I TTAA 9 cut(s) 716, 791, 876, 1428, 1938, 2169, 2751, 3083, 3150
Tru9I TTAA 9 cut(s) 716, 791, 876, 1428, 1938, 2169, 2751, 3083, 3150
TscAI CASTG 5 cut(s) 1689, 2031, 2685, 2934, 3151
TseFI GTSAC 4 cut(s) 586, 1821, 2881, 3073
TseI GCWGC 8 cut(s) 187, 464, 608, 1390, 1474, 1742, 2990, 3002
Tsp45I GTSAC 4 cut(s) 586, 1821, 2881, 3073
TspDTI ATGAA 8 cut(s) 254, 873, 1002, 1038, 1449, 2016, 2460, 2787
TspGWI ACGGA 3 cut(s) 605, 1840, 3233
TspRI CASTG 5 cut(s) 1689, 2031, 2685, 2934, 3151
Tth111I GACNNNGTC 1 cut(s) 980
Van91I CCANNNNNTGG 1 cut(s) 346
Vha464I CTTAAG 1 cut(s) 875
VpaK11BI GGWCC 4 cut(s) 39, 77, 1807, 3354
VspI ATTAAT 1 cut(s) 1938
XagI CCTNNNNNAGG 2 cut(s) 899, 3068
XapI RAATTY 9 cut(s) 427, 828, 1066, 1321, 1330, 1437, 1479, 2204, 2708
XbaI TCTAGA 3 cut(s) 646, 2657, 2666
XceI RCATGY 3 cut(s) 524, 1799, 2351
XmiI GTMKAC 3 cut(s) 72, 506, 3060
XmnI GAANNNNTTC 2 cut(s) 1352, 2208
Zsp2I ATGCAT 1 cut(s) 526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.