RchiOBHm_Chr4g0436101

nuclear matrix constituent protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0001591
Physical Location & Seq
Forward (+)
59464309 .. 59466454
2146 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40447

Sequence Viewer

Length: 477 bp
ATGATTGTGATAGCGAAGGACGTTCTGGTAGTGTCACAGCTGGTGGGCATAGGAAGAGGCGCCAACCCGTTGCTTCAGCAGTGCAAACCCCTGGAGAACAGTGATATAATCTCAGACACCGCAAGACGCACATTGGCAGCAGCATCAGCAGCGCCTCACCTGAAAAGTAGAAGGAAGGAAGAATCAAATCCTGAATCTGTTGGTGTAGACCTGATTCAGGTCACAACGTTAAAAACTGTGGAGTCAACAGAGGAACGAGTTGTGAGGTTTGCAACACCAGAACCACAAGACACTGTTAATGGCAATGCTGATGCTGATGAAACGAAGTTGGTTGAAGAGAGAGAGTTGAGTACAGAAGTGAATGGCACAGAATCTAGTGGTGGCAACAGCACAGGCGGGGAGAGTGACAACAGCACAGGTGATGAGAGCGGCGATGACTATGATGATGAGGATCCAATCCTGGTCAAGCGTCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

158

Amino Acids

16.89

Weight (kDa)

4.37

Isoelectric Point (pI)

40.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 59
AccBSI CCGCTC 1 cut(s) 429
AccI GTMKAC 1 cut(s) 207
AciI CCGC 3 cut(s) 120, 396, 429
AclI AACGTT 1 cut(s) 227
AclWI GGATC 2 cut(s) 446, 459
AcuI CTGAAG 1 cut(s) 59
AcyI GRCGYC 1 cut(s) 60
AfaI GTAC 1 cut(s) 352
AfiI CCNNNNNNNGG 1 cut(s) 217
AgsI TTSAA 1 cut(s) 335
AjnI CCWGG 2 cut(s) 90, 459
AluBI AGCT 1 cut(s) 40
AluI AGCT 1 cut(s) 40
AlwI GGATC 2 cut(s) 446, 459
ApeKI GCWGC 3 cut(s) 137, 140, 149
AspLEI GCGC 2 cut(s) 62, 154
AsuHPI GGTGA 2 cut(s) 149, 431
BamHI GGATCC 1 cut(s) 451
BanI GGYRCC 1 cut(s) 59
BbvI GCAGC 3 cut(s) 149, 152, 161
BciT130I CCWGG 2 cut(s) 92, 461
BfaI CTAG 1 cut(s) 375
BfoI RGCGCY 2 cut(s) 63, 155
BisI GCNGC 4 cut(s) 138, 141, 150, 430
BlsI GCNGC 4 cut(s) 139, 142, 151, 431
Bme1390I CCNGG 2 cut(s) 92, 461
BmiI GGNNCC 2 cut(s) 61, 453
BmrFI CCNGG 2 cut(s) 92, 461
BmsI GCATC 2 cut(s) 152, 301
BpmI CTGGAG 1 cut(s) 113
BsaBI GATNNNNATC 1 cut(s) 450
BsaHI GRCGYC 1 cut(s) 60
BsaJI CCNNGG 1 cut(s) 90
Bsc4I CCNNNNNNNGG 1 cut(s) 217
Bse3DI GCAATG 1 cut(s) 310
Bse8I GATNNNNATC 1 cut(s) 450
BseBI CCWGG 2 cut(s) 92, 461
BseDI CCNNGG 1 cut(s) 90
BseJI GATNNNNATC 1 cut(s) 450
BseLI CCNNNNNNNGG 1 cut(s) 217
BseMI GCAATG 1 cut(s) 310
BseMII CTCAG 1 cut(s) 126
BseXI GCAGC 3 cut(s) 149, 152, 161
BshNI GGYRCC 1 cut(s) 59
BslI CCNNNNNNNGG 1 cut(s) 217
Bsp143I GATC 1 cut(s) 451
BspACI CCGC 3 cut(s) 120, 396, 429
BspCNI CTCAG 1 cut(s) 125
BspLI GGNNCC 2 cut(s) 61, 453
BspPI GGATC 2 cut(s) 446, 459
BspT107I GGYRCC 1 cut(s) 59
BsrBI CCGCTC 1 cut(s) 429
BsrDI GCAATG 1 cut(s) 310
BssECI CCNNGG 1 cut(s) 90
BssMI GATC 1 cut(s) 451
BssNI GRCGYC 1 cut(s) 60
Bst2UI CCWGG 2 cut(s) 92, 461
Bst4CI ACNGT 3 cut(s) 101, 238, 295
Bst6I CTCTTC 2 cut(s) 49, 330
BstACI GRCGYC 1 cut(s) 60
BstDEI CTNAG 1 cut(s) 112
BstENI CCTNNNNNAGG 1 cut(s) 215
BstH2I RGCGCY 2 cut(s) 63, 155
BstHHI GCGC 2 cut(s) 62, 154
BstKTI GATC 1 cut(s) 454
BstMBI GATC 1 cut(s) 451
BstMWI GCNNNNNNNGC 2 cut(s) 146, 149
BstNI CCWGG 2 cut(s) 92, 461
BstSCI CCNGG 2 cut(s) 90, 459
BstV1I GCAGC 3 cut(s) 149, 152, 161
BstX2I RGATCY 1 cut(s) 451
BstYI RGATCY 1 cut(s) 451
BtgZI GCGATG 1 cut(s) 447
BtsI GCAGTG 1 cut(s) 86
BtsIMutI CAGTG 3 cut(s) 86, 106, 291
CfoI GCGC 2 cut(s) 62, 154
CseI GACGC 2 cut(s) 135, 458
Csp6I GTAC 1 cut(s) 351
CviJI RGCY 1 cut(s) 40
CviKI_1 RGCY 1 cut(s) 40
CviQI GTAC 1 cut(s) 351
DdeI CTNAG 1 cut(s) 112
DinI GGCGCC 1 cut(s) 61
DpnI GATC 1 cut(s) 453
DpnII GATC 1 cut(s) 451
Eam1104I CTCTTC 2 cut(s) 49, 330
EarI CTCTTC 2 cut(s) 49, 330
Eco57I CTGAAG 1 cut(s) 59
EcoNI CCTNNNNNAGG 1 cut(s) 215
EcoRII CCWGG 2 cut(s) 90, 459
EgeI GGCGCC 1 cut(s) 61
EheI GGCGCC 1 cut(s) 61
FaiI YATR 3 cut(s) 50, 107, 441
FauI CCCGC 1 cut(s) 389
FblI GTMKAC 1 cut(s) 207
Fnu4HI GCNGC 4 cut(s) 138, 141, 150, 430
Fsp4HI GCNGC 4 cut(s) 138, 141, 150, 430
FspBI CTAG 1 cut(s) 375
GlaI GCGC 2 cut(s) 61, 153
GluI GCNGC 4 cut(s) 138, 141, 150, 430
GsuI CTGGAG 1 cut(s) 113
HaeII RGCGCY 2 cut(s) 63, 155
HgaI GACGC 2 cut(s) 135, 458
HhaI GCGC 2 cut(s) 62, 154
Hin1I GRCGYC 1 cut(s) 60
Hin6I GCGC 2 cut(s) 60, 152
HinP1I GCGC 2 cut(s) 60, 152
HincII GTYRAC 1 cut(s) 246
HindII GTYRAC 1 cut(s) 246
HinfI GANTC 5 cut(s) 182, 194, 214, 242, 371
HphI GGTGA 2 cut(s) 149, 431
Hpy166II GTNNAC 2 cut(s) 208, 246
Hpy188I TCNGA 1 cut(s) 115
Hpy188III TCNNGA 1 cut(s) 191
Hpy8I GTNNAC 2 cut(s) 208, 246
HpyAV CCTTC 3 cut(s) 10, 165, 169
HpyCH4III ACNGT 3 cut(s) 101, 238, 295
HpyCH4IV ACGT 2 cut(s) 21, 227
HpyCH4V TGCA 2 cut(s) 84, 272
HpyF10VI GCNNNNNNNGC 2 cut(s) 146, 149
HpyF3I CTNAG 1 cut(s) 112
HpySE526I ACGT 2 cut(s) 21, 227
Hsp92I GRCGYC 1 cut(s) 60
HspAI GCGC 2 cut(s) 60, 152
KasI GGCGCC 1 cut(s) 59
Kzo9I GATC 1 cut(s) 451
Lsp1109I GCAGC 3 cut(s) 149, 152, 161
LweI GCATC 2 cut(s) 152, 301
MaeI CTAG 1 cut(s) 375
MaeII ACGT 2 cut(s) 21, 227
MaeIII GTNAC 3 cut(s) 33, 220, 404
MalI GATC 1 cut(s) 453
MbiI CCGCTC 1 cut(s) 429
MboI GATC 1 cut(s) 451
MboII GAAGA 3 cut(s) 66, 191, 347
MflI RGATCY 1 cut(s) 451
Mly113I GGCGCC 1 cut(s) 60
MlyI GAGTC 1 cut(s) 251
MnlI CCTC 5 cut(s) 50, 165, 244, 258, 442
MseI TTAA 2 cut(s) 230, 297
MspA1I CMGCKG 1 cut(s) 40
MspR9I CCNGG 2 cut(s) 92, 461
MvaI CCWGG 2 cut(s) 92, 461
MwoI GCNNNNNNNGC 2 cut(s) 146, 149
NarI GGCGCC 1 cut(s) 60
NdeII GATC 1 cut(s) 451
NlaIV GGNNCC 2 cut(s) 61, 453
NmuCI GTSAC 3 cut(s) 33, 220, 404
PfeI GAWTC 4 cut(s) 182, 194, 214, 371
PkrI GCNGC 4 cut(s) 139, 142, 151, 431
PleI GAGTC 1 cut(s) 250
PluTI GGCGCC 1 cut(s) 63
PpsI GAGTC 1 cut(s) 250
Psp1406I AACGTT 1 cut(s) 227
Psp6I CCWGG 2 cut(s) 90, 459
PspGI CCWGG 2 cut(s) 90, 459
PspN4I GGNNCC 2 cut(s) 61, 453
PsuI RGATCY 1 cut(s) 451
PvuII CAGCTG 1 cut(s) 40
RsaI GTAC 1 cut(s) 352
RsaNI GTAC 1 cut(s) 351
SaqAI TTAA 2 cut(s) 230, 297
SatI GCNGC 4 cut(s) 138, 141, 150, 430
Sau3AI GATC 1 cut(s) 451
SchI GAGTC 1 cut(s) 251
ScrFI CCNGG 2 cut(s) 92, 461
SetI ASST 8 cut(s) 24, 42, 162, 213, 222, 230, 269, 421
SfaNI GCATC 2 cut(s) 152, 301
SfoI GGCGCC 1 cut(s) 61
SsiI CCGC 3 cut(s) 120, 396, 429
SspDI GGCGCC 1 cut(s) 59
SspMI CTAG 1 cut(s) 375
StyD4I CCNGG 2 cut(s) 90, 459
TaaI ACNGT 3 cut(s) 101, 238, 295
TaiI ACGT 2 cut(s) 24, 230
TatI WGTACW 1 cut(s) 350
TauI GCSGC 1 cut(s) 432
TfiI GAWTC 4 cut(s) 182, 194, 214, 371
Tru1I TTAA 2 cut(s) 230, 297
Tru9I TTAA 2 cut(s) 230, 297
TscAI CASTG 3 cut(s) 86, 106, 298
TseFI GTSAC 3 cut(s) 33, 220, 404
TseI GCWGC 3 cut(s) 137, 140, 149
Tsp45I GTSAC 3 cut(s) 33, 220, 404
TspDTI ATGAA 1 cut(s) 333
TspRI CASTG 3 cut(s) 86, 106, 298
XagI CCTNNNNNAGG 1 cut(s) 215
XmiI GTMKAC 1 cut(s) 207
XspI CTAG 1 cut(s) 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.