RchiOBHm_Chr7g0226101

nuclear matrix constituent protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001591
Physical Location & Seq
Forward (+)
49133098 .. 49138954
5857 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20251

Sequence Viewer

Length: 3456 bp
ATGTTCACGCCGCTGAGGAAGGCGCCGGCGACGCCGAGGACCACCGACAAGGGCAAGGCCGTGGCGTACGTCGACGGTCCTCCGCCGCCACTTGGTTCTCTGAGCGAAATCAGAAGCGGCGGCGGTGGGGCCAAAACGTTGCCGGATTTGGACAATGCCGATTGGAGGCGGTTTAAGGAGGTTGGGCTGCTCGATGAGGCGGCCATGGAGCGCCGGGACCGCCAAGAACTCGCCAATAAGGTCGCTAGGCTTGAGTCTGAGCTTCATGATTACCAACATAGCATGGGGCTTCTCCTCATTGAGAAGAAAGAATGGGCATTGGAGCATGAGGAGTTGAGCCAAGCATTGGCTGAAACCCATGAGATTCTACACCGGGAGCAGAGGGCTCATCTGATTGCAATTTCGGAAGTCGAAAGGCGAGAGGAGAATTTGAGGAAGATATTGGTGGAGGAAAAGAGGTCTGCAGCTGAGCTCGAAAAGTCATTGCGTCAAATGCATGAGGAGTATACCCGGATAAAGCATGCATCTGAGGCGAAGGTGGCTGATGCGAATGCGTTGATTGTTAGTGTTGAAGATAAATCTCTAGTGACAGATGAGAAGTTTCTTGCTGCTGAAACCAAGCTTGCTGAGGCCAACAAAAAGAGTCTAGAGGTGGAGAGGAGGTTGCAAGAGGTGGAGACACGTGAGAGTGTGCTTCGGAGGGAGCAAGCGTCATTAAGTACAGAGAGAGAGGCACACAAGACAACTTTTTACAGACAAAGAGAAGACTTGAACGAGTGGGAGAAGAAGTTAAAAGAAGGGGAAGAGAGGCTAAGCAATCTTCGTAAAATTTTGAATGAGAAAGAGGAAAAAACAAATGAAAACGATATGATTCTTAAGCAAAAAGAGAAAGACCTTTATGAGGCTGAAAGGAAGATTGAGTCTTCAAACTCTCTTTTGAAAGAGAAGGAAGATGATGTGAATAAACGCCAAGCTGACCTAGTCTCAAAGGAAAAGGAGGTTGATTCAGCTTCATACATCTTAGAGACGAAGGAGAAAGAATTAAATGCATTGGAGGAAAAACTAAATTCAAGGGAAAATGTGGAGATTCAAGAGCATCTTGACCAGCACAGGGTCATTCTTGATAGAAAAATGCAGGCTTTTGAGTTGGGAATTGAGGAAAGGAGGAACTCTTTTGAGAAGGAACAGAGTAGCAAGATAGAAGCGGTGGAACAAAAGGAACTTGAAATCAGTCATAAGGAAGAAATGTTGAAGAAGCAAGAGAAAGCGTTGGATGAGAAATCAGAGAGGCTGAAGGAGAAAAATAAGGAAGTTGAAACAAATTTGAAAAATCTGAAGGAGAAAGAGAAGACCTTCAAAGCTGATGAGAAAAAGTTAGAGCTGGAAAGGCAGCAAATACTTACTGATATAGAGCACCTTCAGAATCTTAAAGATGAAATTCAGAAAATAAAAGACGAAAATGCTCAACTGGAGCAGCAAATTCGTGAAGGCAGAGAGAAGCAGGGAATTACCGAGAAAGAGAGGTCGGACCACGTACGTTTGCAGTCAGAACTGCAACAGGAGATAAACAATTATAGACTTCAGAATGAGTTACTTTTGAAGGAAGCTGAAGATCTGAAGCAGGAGAGGGAAAAGTTTGAAAAAGAGTGGGAAGATTTGGATGAAAGAAGAGCTAAAGTTGATGTAGAGCTTAGAAAAGTTGTTGAAGAGAAAGAAAAATTGGAAAGATTACAATGTATTGAAGCAGAGAGGTTGAAAGTAGAGAGACAAGCAGTGCAAGATTACAGACAGAGGGAGATGGAAAATCTCAAGCAGGAGAAAGAATCATTTGCAGCTAAGATGAGTAATGAGCAGATAGCCTTATCTGAAAAGGCCCAATCTGAGCATGCTCAAATGGTCCAAGATTTTGAGTCACGGAGAAGAGATCTTGAGATTGATATGCAGAATAGGCAGGATAAGATGGTAAAACAACTGCAGGAAAGGGAGAGAGCATTTGAGGAGGAGAAGGATAGAGAACATACTAATATTAATTTCTTGAAGGGAGTTGCTGATAAGCAAGGAGAAGAGCTATTATCTGAAAGGAATACGAATGAAAAGGAAAGGGAAGCACTTGCACTGCAGAAAAAGGAGCTGGAAGCGAACCAACTTGAAATGCGAGAAGACATAGATCAGCTTGACATGCTTAGCAAGAAGATCAAGTGCCAGAGGGAACAGCTGATTGAGGAAAGAGGTCGCTTTCTTGCCTTTGTTGAGAAGGTTAAGAGTTGCAAGGACTGTGGAGAAATAACAAGGGAATTTGTTCTCTCTGATCTCCAAGTACCTGGGATGTACCATGCTGACGCTGTTCCTGATTCTGAACATAAAGAGTCAGGATGGGGGAAAAAACTGCAACAGAAATGCAAGTTAGTAGTTTCCAAAGTAACATCAAATAAAATACATGTTGTATCTACCGAGTTACCACCACTATCGGCTATGCAGAAAGGAAAGGAACCCAACTTGCTTGCTGATGAGGAAGCAAGAGGTGCTAGCAGTCATGAAGAGCCACAGCCATCTGTTAGGAGATGTAATGACTCGGCCAATGCTCAAGCAGTCGTAAATGATAACAACTCTAAGAAGGTGGATGATGGGTATGCTCCATCAATTGACGATCACAGTTTCGTTGACAGCCAGGAGCAAGACATTCCAGAAGATTCTGAGCAATCAGAGCTGAAAAGTGGCCGAAGAAAACCAGCTAGGGGACGCAAGTCTAGATTGTCTAGAACACACTCAGTGAAGGAAGTTGTTGAAGACGCAAAGAAATTTTTAGGAGAAACTCCAGAAGCATCAAATGCAAGTTTGCTTAATGAGAGTAGTTACATAAATGAAGGTGATTCCAGTTTTACGAGCATTGGTCGGAAACGAGCCCGCCCTCAGAGCTCCAGGATAGAAAGCGAGCAAGATGATTGTGATAGCGAAGGACGTTCTGGTAGTGTCACAGCTGGTGGGCGTAGGAAGAGGCGCCAACCCGTTGCTTCAGCAGTGCAAACCCCTGGAGAACAGCGATATAATCTCAGACACCGCAAGACTGCAGGCACATTGGCAGGAACATCAGCAGCGCCTCACCTGAAAAGTACAAGGAAGGAAGAATCAAATCCTGAATCTGTTGGTGTAGACCTGATACAGGTCACAACATTAAAAACTGTGGAGTCAACAGAGGAGAGAGTTGTGAGGTTTGCAACACCAGAACCACAAGACACTGTTAATGGCAATGCTGATGCTGATGCTGATGCAACGAAGTTGGTTGAAGAGACAGAGTTGAGTACGGAAGTGAATGGCACAGAATCTAGTGGTGGCAACAGCACAGGCGGTGAGAGTGACAACAGCACAGGCGATGAGAGCGGCGATGACTATGATGATGAGGATCATCCTGGTCAAGCGTCAATAGGGAAGAAGATCTGGACCTTCTTCAGCACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1151

Amino Acids

132.34

Weight (kDa)

5.25

Isoelectric Point (pI)

54.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 22, 2997
AccB7I CCANNNNNTGG 1 cut(s) 346
AccBSI CCGCTC 1 cut(s) 3378
AccI GTMKAC 3 cut(s) 72, 506, 3150
AclI AACGTT 1 cut(s) 137
AclWI GGATC 1 cut(s) 3408
AcoI YGGCCR 3 cut(s) 201, 2574, 2716
AcsI RAATTY 8 cut(s) 427, 828, 1066, 1321, 1437, 1479, 2294, 2798
AcuI CTGAAG 8 cut(s) 1313, 1355, 1403, 1565, 1629, 1637, 2997, 3430
AcvI CACGTG 1 cut(s) 683
AcyI GRCGYC 3 cut(s) 23, 32, 2998
AdeI CACNNNGTG 1 cut(s) 2770
AfaI GTAC 7 cut(s) 68, 721, 1536, 2319, 2330, 3112, 3301
AfiI CCNNNNNNNGG 7 cut(s) 92, 165, 346, 901, 1111, 2735, 3160
AflII CTTAAG 1 cut(s) 875
AflIII ACRYGT 2 cut(s) 680, 2437
AjnI CCWGG 5 cut(s) 2320, 2667, 2918, 3028, 3406
AjuI GAANNNNNNNTTGG 2 cut(s) 1703, 1735
Alw21I GWGCWC 3 cut(s) 474, 1416, 2918
Alw26I GTCTC 5 cut(s) 671, 988, 1019, 1759, 3281
AlwI GGATC 1 cut(s) 3408
AoxI GGCC 7 cut(s) 57, 129, 201, 630, 1872, 2574, 2716
ApeKI GCWGC 7 cut(s) 187, 464, 608, 1390, 1474, 1832, 3092
ApoI RAATTY 8 cut(s) 427, 828, 1066, 1321, 1437, 1479, 2294, 2798
AseI ATTAAT 1 cut(s) 2028
Asp700I GAANNNNTTC 2 cut(s) 1352, 2298
AspLEI GCGC 4 cut(s) 25, 213, 3000, 3097
AspS9I GGNCC 8 cut(s) 39, 77, 129, 217, 1528, 1873, 1897, 3438
AsuC2I CCSGG 3 cut(s) 215, 374, 511
AsuHPI GGTGA 3 cut(s) 2879, 3092, 3359
AsuNHI GCTAGC 1 cut(s) 2525
AvaII GGWCC 6 cut(s) 39, 77, 217, 1528, 1897, 3438
BanI GGYRCC 2 cut(s) 22, 2997
BanII GRGCYC 4 cut(s) 388, 474, 2905, 2918
BbrPI CACGTG 1 cut(s) 683
BbsI GAAGAC 5 cut(s) 771, 915, 1355, 2166, 2793
Bbv12I GWGCWC 3 cut(s) 474, 1416, 2918
BbvCI CCTCAGC 2 cut(s) 14, 627
BbvI GCAGC 7 cut(s) 174, 476, 595, 1402, 1486, 1844, 3104
BccI CCATC 6 cut(s) 1792, 1954, 2367, 2557, 2618, 2644
BceAI ACGGC 1 cut(s) 44
BcgI CGANNNNNNTGC 2 cut(s) 1463, 1497
BciT130I CCWGG 5 cut(s) 2322, 2669, 2920, 3030, 3408
BcnI CCSGG 3 cut(s) 215, 374, 511
BcoDI GTCTC 5 cut(s) 671, 988, 1019, 1759, 3281
BfaI CTAG 9 cut(s) 246, 584, 647, 980, 2526, 2733, 2748, 2757, 3324
BfmI CTRYAG 4 cut(s) 462, 1973, 2117, 3066
BfoI RGCGCY 4 cut(s) 26, 214, 3001, 3098
BfrI CTTAAG 1 cut(s) 875
BglII AGATCT 3 cut(s) 1612, 1924, 3432
BlpI GCTNAGC 3 cut(s) 468, 812, 2183
Bme1390I CCNGG 8 cut(s) 215, 374, 511, 2322, 2669, 2920, 3030, 3408
Bme18I GGWCC 6 cut(s) 39, 77, 217, 1528, 1897, 3438
BmgT120I GGNCC 8 cut(s) 39, 77, 129, 217, 1528, 1873, 1897, 3438
BmiI GGNNCC 5 cut(s) 24, 130, 218, 2490, 2999
BmrFI CCNGG 8 cut(s) 215, 374, 511, 2322, 2669, 2920, 3030, 3408
BmsI GCATC 7 cut(s) 533, 535, 1105, 2831, 3244, 3250, 3256
BmtI GCTAGC 1 cut(s) 2529
BoxI GACNNNNGTC 1 cut(s) 2743
BpiI GAAGAC 5 cut(s) 771, 915, 1355, 2166, 2793
BpmI CTGGAG 4 cut(s) 1490, 2799, 2902, 3051
Bpu10I CCTNAGC 2 cut(s) 14, 627
Bpu1102I GCTNAGC 3 cut(s) 468, 812, 2183
BpuEI CTTGAG 4 cut(s) 272, 1793, 1949, 2568
BpuMI CCSGG 3 cut(s) 215, 374, 511
BsaAI YACGTR 2 cut(s) 683, 1534
BsaBI GATNNNNATC 1 cut(s) 3399
BsaHI GRCGYC 3 cut(s) 23, 32, 2998
BsaJI CCNNGG 5 cut(s) 35, 60, 204, 2321, 3028
BsaXI ACNNNNNCTCC 6 cut(s) 647, 677, 695, 725, 773, 803
Bsc4I CCNNNNNNNGG 7 cut(s) 92, 165, 346, 901, 1111, 2735, 3160
Bse118I RCCGGY 1 cut(s) 25
Bse1I ACTGG 2 cut(s) 1473, 2874
Bse3DI GCAATG 2 cut(s) 482, 3253
Bse8I GATNNNNATC 1 cut(s) 3399
BseBI CCWGG 5 cut(s) 2322, 2669, 2920, 3030, 3408
BseDI CCNNGG 5 cut(s) 35, 60, 204, 2321, 3028
BseGI GGATG 6 cut(s) 1279, 1666, 2331, 2378, 2626, 3403
BseJI GATNNNNATC 1 cut(s) 3399
BseLI CCNNNNNNNGG 7 cut(s) 92, 165, 346, 901, 1111, 2735, 3160
BseMI GCAATG 2 cut(s) 482, 3253
BseNI ACTGG 2 cut(s) 1473, 2874
BseRI GAGGAG 8 cut(s) 284, 344, 437, 515, 673, 2012, 2015, 3209
BseXI GCAGC 7 cut(s) 174, 476, 595, 1402, 1486, 1844, 3104
BshFI GGCC 7 cut(s) 59, 131, 203, 632, 1874, 2576, 2718
BshNI GGYRCC 2 cut(s) 22, 2997
BsiHKAI GWGCWC 3 cut(s) 474, 1416, 2918
BsiSI CCGG 5 cut(s) 26, 143, 214, 373, 511
BsiWI CGTACG 2 cut(s) 66, 1534
BslFI GGGAC 2 cut(s) 230, 2751
BslI CCNNNNNNNGG 7 cut(s) 92, 165, 346, 901, 1111, 2735, 3160
BsmAI GTCTC 5 cut(s) 671, 988, 1019, 1759, 3281
BsmBI CGTCTC 1 cut(s) 1019
BsmFI GGGAC 2 cut(s) 230, 2751
BsmI GAATGC 1 cut(s) 556
BsnI GGCC 7 cut(s) 59, 131, 203, 632, 1874, 2576, 2718
Bsp1286I GDGCHC 5 cut(s) 388, 474, 1416, 2905, 2918
Bsp143I GATC 8 cut(s) 1612, 1924, 2167, 2193, 2308, 2647, 3400, 3432
Bsp1720I GCTNAGC 3 cut(s) 468, 812, 2183
Bsp19I CCATGG 1 cut(s) 204
BspANI GGCC 7 cut(s) 59, 131, 203, 632, 1874, 2576, 2718
BspHI TCATGA 2 cut(s) 265, 2533
BspLI GGNNCC 5 cut(s) 24, 130, 218, 2490, 2999
BspMAI CTGCAG 4 cut(s) 466, 1977, 2121, 3070
BspOI GCTAGC 1 cut(s) 2529
BspPI GGATC 1 cut(s) 3408
BspQI GCTCTTC 3 cut(s) 1663, 2058, 2532
BspT107I GGYRCC 2 cut(s) 22, 2997
BspTI CTTAAG 1 cut(s) 875
BsrBI CCGCTC 1 cut(s) 3378
BsrDI GCAATG 2 cut(s) 482, 3253
BsrFI RCCGGY 1 cut(s) 25
BsrI ACTGG 2 cut(s) 1473, 2874
BssAI RCCGGY 1 cut(s) 25
BssECI CCNNGG 5 cut(s) 35, 60, 204, 2321, 3028
BssMI GATC 8 cut(s) 1612, 1924, 2167, 2193, 2308, 2647, 3400, 3432
BssNAI GTATAC 1 cut(s) 507
BssNI GRCGYC 3 cut(s) 23, 32, 2998
BssT1I CCWWGG 1 cut(s) 204
Bst1107I GTATAC 1 cut(s) 507
Bst2UI CCWGG 5 cut(s) 2322, 2669, 2920, 3030, 3408
Bst4CI ACNGT 5 cut(s) 77, 2276, 2654, 3181, 3238
Bst6I CTCTTC 8 cut(s) 798, 1663, 1701, 1915, 2058, 2532, 2987, 3279
BstACI GRCGYC 3 cut(s) 23, 32, 2998
BstAFI CTTAAG 1 cut(s) 875
BstAPI GCANNNNNTGC 2 cut(s) 2522, 2828
BstBAI YACGTR 2 cut(s) 683, 1534
BstDSI CCRYGG 2 cut(s) 60, 204
BstENI CCTNNNNNAGG 2 cut(s) 899, 3158
BstF5I GGATG 6 cut(s) 1279, 1666, 2331, 2378, 2626, 3403
BstH2I RGCGCY 4 cut(s) 26, 214, 3001, 3098
BstHHI GCGC 4 cut(s) 25, 213, 3000, 3097
BstKTI GATC 8 cut(s) 1615, 1927, 2170, 2196, 2311, 2650, 3403, 3435
BstMAI GTCTC 5 cut(s) 671, 988, 1019, 1759, 3281
BstMBI GATC 8 cut(s) 1612, 1924, 2167, 2193, 2308, 2647, 3400, 3432
BstNI CCWGG 5 cut(s) 2322, 2669, 2920, 3030, 3408
BstNSI RCATGY 4 cut(s) 524, 1889, 2182, 2441
BstPAI GACNNNNGTC 1 cut(s) 2743
BstSCI CCNGG 8 cut(s) 213, 372, 509, 2320, 2667, 2918, 3028, 3406
BstSFI CTRYAG 4 cut(s) 462, 1973, 2117, 3066
BstV1I GCAGC 7 cut(s) 174, 476, 595, 1402, 1486, 1844, 3104
BstV2I GAAGAC 5 cut(s) 771, 915, 1355, 2166, 2793
BstX2I RGATCY 3 cut(s) 1612, 1924, 3432
BstXI CCANNNNNNTGG 1 cut(s) 2321
BstYI RGATCY 3 cut(s) 1612, 1924, 3432
BstZ17I GTATAC 1 cut(s) 507
BsuRI GGCC 7 cut(s) 59, 131, 203, 632, 1874, 2576, 2718
BtgI CCRYGG 2 cut(s) 60, 204
BtgZI GCGATG 2 cut(s) 3384, 3396
BtsCI GGATG 6 cut(s) 1279, 1666, 2331, 2378, 2626, 3403
BtsI GCAGTG 3 cut(s) 1779, 2114, 3024
BtsIMutI CAGTG 5 cut(s) 1779, 2114, 2775, 3024, 3234
CciI TCATGA 2 cut(s) 265, 2533
CfoI GCGC 4 cut(s) 25, 213, 3000, 3097
Cfr10I RCCGGY 1 cut(s) 25
Cfr13I GGNCC 8 cut(s) 39, 77, 129, 217, 1528, 1873, 1897, 3438
CseI GACGC 7 cut(s) 40, 476, 699, 2348, 2748, 2798, 3405
Csp6I GTAC 7 cut(s) 67, 720, 1535, 2318, 2329, 3111, 3300
CspCI CAANNNNNGTGG 2 cut(s) 2257, 2292
CviQI GTAC 7 cut(s) 67, 720, 1535, 2318, 2329, 3111, 3300
DinI GGCGCC 2 cut(s) 24, 2999
DpnI GATC 8 cut(s) 1614, 1926, 2169, 2195, 2310, 2649, 3402, 3434
DpnII GATC 8 cut(s) 1612, 1924, 2167, 2193, 2308, 2647, 3400, 3432
DraIII CACNNNGTG 1 cut(s) 2770
EaeI YGGCCR 3 cut(s) 201, 2574, 2716
Eam1104I CTCTTC 8 cut(s) 798, 1663, 1701, 1915, 2058, 2532, 2987, 3279
EarI CTCTTC 8 cut(s) 798, 1663, 1701, 1915, 2058, 2532, 2987, 3279
EciI GGCGGA 1 cut(s) 72
Ecl136II GAGCTC 2 cut(s) 472, 2916
Eco130I CCWWGG 1 cut(s) 204
Eco24I GRGCYC 4 cut(s) 388, 474, 2905, 2918
Eco47I GGWCC 6 cut(s) 39, 77, 217, 1528, 1897, 3438
Eco53kI GAGCTC 2 cut(s) 472, 2916
Eco57I CTGAAG 8 cut(s) 1313, 1355, 1403, 1565, 1629, 1637, 2997, 3430
Eco72I CACGTG 1 cut(s) 683
EcoICRI GAGCTC 2 cut(s) 472, 2916
EcoNI CCTNNNNNAGG 2 cut(s) 899, 3158
EcoRII CCWGG 5 cut(s) 2320, 2667, 2918, 3028, 3406
EcoT14I CCWWGG 1 cut(s) 204
EcoT22I ATGCAT 3 cut(s) 498, 526, 1051
EcoT38I GRGCYC 4 cut(s) 388, 474, 2905, 2918
EgeI GGCGCC 2 cut(s) 24, 2999
EheI GGCGCC 2 cut(s) 24, 2999
ErhI CCWWGG 1 cut(s) 204
Esp3I CGTCTC 1 cut(s) 1019
FalI AAGNNNNNCTT 2 cut(s) 1083, 1115
FaqI GGGAC 2 cut(s) 230, 2751
FauI CCCGC 1 cut(s) 2912
FblI GTMKAC 3 cut(s) 72, 506, 3150
FokI GGATG 6 cut(s) 1286, 1673, 2338, 2385, 2633, 3390
FriOI GRGCYC 4 cut(s) 388, 474, 2905, 2918
FspBI CTAG 9 cut(s) 246, 584, 647, 980, 2526, 2733, 2748, 2757, 3324
GlaI GCGC 4 cut(s) 24, 212, 2999, 3096
GsuI CTGGAG 4 cut(s) 1490, 2799, 2902, 3051
HaeII RGCGCY 4 cut(s) 26, 214, 3001, 3098
HaeIII GGCC 7 cut(s) 59, 131, 203, 632, 1874, 2576, 2718
HapII CCGG 5 cut(s) 26, 143, 214, 373, 511
HgaI GACGC 7 cut(s) 40, 476, 699, 2348, 2748, 2798, 3405
HhaI GCGC 4 cut(s) 25, 213, 3000, 3097
Hin1I GRCGYC 3 cut(s) 23, 32, 2998
Hin6I GCGC 4 cut(s) 23, 211, 2998, 3095
HinP1I GCGC 4 cut(s) 23, 211, 2998, 3095
HincII GTYRAC 3 cut(s) 73, 2662, 3189
HindII GTYRAC 3 cut(s) 73, 2662, 3189
HindIII AAGCTT 1 cut(s) 620
HpaII CCGG 5 cut(s) 26, 143, 214, 373, 511
HphI GGTGA 3 cut(s) 2879, 3092, 3359
Hpy166II GTNNAC 6 cut(s) 6, 73, 507, 2662, 3151, 3189
Hpy8I GTNNAC 6 cut(s) 6, 73, 507, 2662, 3151, 3189
Hpy99I CGWCG 3 cut(s) 34, 74, 77
HpyCH4III ACNGT 5 cut(s) 77, 2276, 2654, 3181, 3238
HpyCH4IV ACGT 6 cut(s) 69, 137, 682, 1533, 1537, 2959
HpySE526I ACGT 6 cut(s) 69, 137, 682, 1533, 1537, 2959
Hsp92I GRCGYC 3 cut(s) 23, 32, 2998
HspAI GCGC 4 cut(s) 23, 211, 2998, 3095
KasI GGCGCC 2 cut(s) 22, 2997
KroI GCCGGC 1 cut(s) 25
KroNI GCCGGC 1 cut(s) 27
Kzo9I GATC 8 cut(s) 1612, 1924, 2167, 2193, 2308, 2647, 3400, 3432
LguI GCTCTTC 3 cut(s) 1663, 2058, 2532
LmnI GCTCC 9 cut(s) 208, 322, 376, 703, 1471, 2128, 2638, 2671, 2921
Lsp1109I GCAGC 7 cut(s) 174, 476, 595, 1402, 1486, 1844, 3104
LweI GCATC 7 cut(s) 533, 535, 1105, 2831, 3244, 3250, 3256
MaeI CTAG 9 cut(s) 246, 584, 647, 980, 2526, 2733, 2748, 2757, 3324
MaeII ACGT 6 cut(s) 69, 137, 682, 1533, 1537, 2959
MaeIII GTNAC 9 cut(s) 586, 1590, 1911, 2419, 2454, 2852, 2971, 3163, 3353
MalI GATC 8 cut(s) 1614, 1926, 2169, 2195, 2310, 2649, 3402, 3434
MbiI CCGCTC 1 cut(s) 3378
MboI GATC 8 cut(s) 1612, 1924, 2167, 2193, 2308, 2647, 3400, 3432
MfeI CAATTG 1 cut(s) 2640
MflI RGATCY 3 cut(s) 1612, 1924, 3432
MhlI GDGCHC 5 cut(s) 388, 474, 1416, 2905, 2918
Mly113I GGCGCC 2 cut(s) 23, 2998
MlyI GAGTC 7 cut(s) 263, 652, 929, 1919, 2375, 2564, 3194
MmeI TCCRAC 3 cut(s) 1251, 1506, 2873
Mph1103I ATGCAT 3 cut(s) 498, 526, 1051
MreI CGCCGGCG 1 cut(s) 25
MroNI GCCGGC 1 cut(s) 25
MroXI GAANNNNTTC 2 cut(s) 1352, 2298
MspA1I CMGCKG 4 cut(s) 13, 467, 2215, 2978
MspCI CTTAAG 1 cut(s) 875
MspI CCGG 5 cut(s) 26, 143, 214, 373, 511
MspR9I CCNGG 8 cut(s) 215, 374, 511, 2322, 2669, 2920, 3030, 3408
MunI CAATTG 1 cut(s) 2640
Mva1269I GAATGC 1 cut(s) 556
MvaI CCWGG 5 cut(s) 2322, 2669, 2920, 3030, 3408
NaeI GCCGGC 1 cut(s) 27
NarI GGCGCC 2 cut(s) 23, 2998
NciI CCSGG 3 cut(s) 215, 374, 511
NcoI CCATGG 1 cut(s) 204
NdeII GATC 8 cut(s) 1612, 1924, 2167, 2193, 2308, 2647, 3400, 3432
NgoMIV GCCGGC 1 cut(s) 25
NheI GCTAGC 1 cut(s) 2525
NlaIV GGNNCC 5 cut(s) 24, 130, 218, 2490, 2999
NmeAIII GCCGAG 2 cut(s) 60, 2552
NmuCI GTSAC 5 cut(s) 586, 1911, 2971, 3163, 3353
NsiI ATGCAT 3 cut(s) 498, 526, 1051
NspI RCATGY 4 cut(s) 524, 1889, 2182, 2441
PaeI GCATGC 2 cut(s) 524, 1889
PagI TCATGA 2 cut(s) 265, 2533
PciI ACATGT 1 cut(s) 2437
PciSI GCTCTTC 3 cut(s) 1663, 2058, 2532
PctI GAATGC 1 cut(s) 556
PdiI GCCGGC 1 cut(s) 27
PdmI GAANNNNTTC 2 cut(s) 1352, 2298
Pfl23II CGTACG 2 cut(s) 66, 1534
PflFI GACNNNGTC 1 cut(s) 980
PflMI CCANNNNNTGG 1 cut(s) 346
PfoI TCCNGGA 1 cut(s) 2918
PleI GAGTC 7 cut(s) 262, 651, 928, 1918, 2374, 2564, 3193
PluTI GGCGCC 2 cut(s) 26, 3001
PmaCI CACGTG 1 cut(s) 683
PmlI CACGTG 1 cut(s) 683
PpsI GAGTC 7 cut(s) 262, 651, 928, 1918, 2374, 2564, 3193
Ppu21I YACGTR 2 cut(s) 683, 1534
PscI ACATGT 1 cut(s) 2437
PshAI GACNNNNGTC 1 cut(s) 2743
PshBI ATTAAT 1 cut(s) 2028
Psp124BI GAGCTC 2 cut(s) 474, 2918
Psp1406I AACGTT 1 cut(s) 137
Psp6I CCWGG 5 cut(s) 2320, 2667, 2918, 3028, 3406
PspCI CACGTG 1 cut(s) 683
PspGI CCWGG 5 cut(s) 2320, 2667, 2918, 3028, 3406
PspLI CGTACG 2 cut(s) 66, 1534
PspN4I GGNNCC 5 cut(s) 24, 130, 218, 2490, 2999
PspPI GGNCC 8 cut(s) 39, 77, 129, 217, 1528, 1873, 1897, 3438
PstI CTGCAG 4 cut(s) 466, 1977, 2121, 3070
PsuI RGATCY 3 cut(s) 1612, 1924, 3432
PsyI GACNNNGTC 1 cut(s) 980
PvuII CAGCTG 3 cut(s) 467, 2215, 2978
RsaI GTAC 7 cut(s) 68, 721, 1536, 2319, 2330, 3112, 3301
RsaNI GTAC 7 cut(s) 67, 720, 1535, 2318, 2329, 3111, 3300
SacI GAGCTC 2 cut(s) 474, 2918
SalI GTCGAC 1 cut(s) 71
SapI GCTCTTC 3 cut(s) 1663, 2058, 2532
Sau3AI GATC 8 cut(s) 1612, 1924, 2167, 2193, 2308, 2647, 3400, 3432
Sau96I GGNCC 8 cut(s) 39, 77, 129, 217, 1528, 1873, 1897, 3438
SchI GAGTC 7 cut(s) 263, 652, 929, 1919, 2375, 2564, 3194
ScrFI CCNGG 8 cut(s) 215, 374, 511, 2322, 2669, 2920, 3030, 3408
SduI GDGCHC 5 cut(s) 388, 474, 1416, 2905, 2918
SfaNI GCATC 7 cut(s) 533, 535, 1105, 2831, 3244, 3250, 3256
SfcI CTRYAG 4 cut(s) 462, 1973, 2117, 3066
SfoI GGCGCC 2 cut(s) 24, 2999
SgrAI CRCCGGYG 1 cut(s) 25
SgrDI CGTCGACG 1 cut(s) 71
SinI GGWCC 6 cut(s) 39, 77, 217, 1528, 1897, 3438
SmlI CTYRAG 5 cut(s) 251, 875, 1808, 1928, 2583
SmoI CTYRAG 5 cut(s) 251, 875, 1808, 1928, 2583
SphI GCATGC 2 cut(s) 524, 1889
SspDI GGCGCC 2 cut(s) 22, 2997
SspI AATATT 1 cut(s) 2026
SspMI CTAG 9 cut(s) 246, 584, 647, 980, 2526, 2733, 2748, 2757, 3324
SstI GAGCTC 2 cut(s) 474, 2918
StyD4I CCNGG 8 cut(s) 213, 372, 509, 2320, 2667, 2918, 3028, 3406
StyI CCWWGG 1 cut(s) 204
TaaI ACNGT 5 cut(s) 77, 2276, 2654, 3181, 3238
TaiI ACGT 6 cut(s) 72, 140, 685, 1536, 1540, 2962
TaqI TCGA 4 cut(s) 72, 192, 411, 474
TatI WGTACW 2 cut(s) 719, 3110
TauI GCSGC 6 cut(s) 13, 88, 120, 123, 203, 3381
TscAI CASTG 5 cut(s) 1779, 2121, 2775, 3024, 3241
TseFI GTSAC 5 cut(s) 586, 1911, 2971, 3163, 3353
TseI GCWGC 7 cut(s) 187, 464, 608, 1390, 1474, 1832, 3092
Tsp45I GTSAC 5 cut(s) 586, 1911, 2971, 3163, 3353
TspDTI ATGAA 8 cut(s) 254, 873, 1002, 1449, 1677, 2106, 2550, 2877
TspGWI ACGGA 2 cut(s) 1930, 3317
TspRI CASTG 5 cut(s) 1779, 2121, 2775, 3024, 3241
Tth111I GACNNNGTC 1 cut(s) 980
Van91I CCANNNNNTGG 1 cut(s) 346
Vha464I CTTAAG 1 cut(s) 875
VpaK11BI GGWCC 6 cut(s) 39, 77, 217, 1528, 1897, 3438
VspI ATTAAT 1 cut(s) 2028
XagI CCTNNNNNAGG 2 cut(s) 899, 3158
XapI RAATTY 8 cut(s) 427, 828, 1066, 1321, 1437, 1479, 2294, 2798
XbaI TCTAGA 3 cut(s) 646, 2747, 2756
XceI RCATGY 4 cut(s) 524, 1889, 2182, 2441
XmiI GTMKAC 3 cut(s) 72, 506, 3150
XmnI GAANNNNTTC 2 cut(s) 1352, 2298
XspI CTAG 9 cut(s) 246, 584, 647, 980, 2526, 2733, 2748, 2757, 3324
Zsp2I ATGCAT 3 cut(s) 498, 526, 1051
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.