Rroxscaffold_5G00377210

nuclear matrix constituent protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001591
Physical Location & Seq
Forward (+)
57736785 .. 57746770
9986 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00377210.1

Sequence Viewer

Length: 735 bp
ATGGGGAAAAAACTGCAACAGAAATGCAAGCTAGTGGTTTCCAAAGTAACTTCAAATAAAAAACATGTTGTATCTACTGACTTACCACCAGTATCGGCTATGCAGAAAGGAAAGGAACCCAACTTTCTTGCTGATGAGGAAGCAAGAGGTCCTAGCAGTCATGAAGAGCCTCAGCCATCTCTTAGGAGATGTAATGACTTGGCCAATGCTCAAGCAGTCATAACTGATAACAACTCTAAGGAGGTGGATGATGGGTATGCTCCATCAATTGACGATCACAGTTTCATTGACAGCCATGAGCAAGACATTCCAGAAGATTCTGAGCAATCAGAGCTGAAAAGTGGCCGACGAAAACCAGCTAGGGGACGCAAGTCTAGATTGTCTAGAGCACACTCAGTGAAGGCAGTTGTTGAAGACGCAAAGAAATTTTTAGGAGAAACTCCAGAAGCATCAAATGCAAATTTGCTCAATGAGAGTAGTTACATAAATGAAGGTGATTCCAGTTTTACGAGCATCGGTCGGAAACAAGCCCGCCCTGAGAGCTCCAGGATAGAAAGTGAGCAAGATGATTGTGATAGCAAAGGACGTTCTGGTAGTGTCACAGCTGGTGGGCATAGGAAGAGGCACCAACCCGTTGCTTCAGCAGTGCAAACCCCTGGAGAACAGCGATATAATCTCAGACACCGCAAGACGCACATTGGCAGCAGCATCAGCGGCGCCTCACCTGAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

244

Amino Acids

26.71

Weight (kDa)

8.67

Isoelectric Point (pI)

63.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 624, 716
AciI CCGC 3 cut(s) 532, 685, 714
AcoI YGGCCR 2 cut(s) 201, 343
AcsI RAATTY 2 cut(s) 425, 460
AcuI CTGAAG 1 cut(s) 624
AcyI GRCGYC 1 cut(s) 717
AdeI CACNNNGTG 1 cut(s) 397
AfiI CCNNNNNNNGG 1 cut(s) 362
AflIII ACRYGT 1 cut(s) 64
AgsI TTSAA 2 cut(s) 54, 413
AjnI CCWGG 2 cut(s) 545, 655
AluBI AGCT 5 cut(s) 31, 334, 359, 543, 605
AluI AGCT 5 cut(s) 31, 334, 359, 543, 605
Alw21I GWGCWC 2 cut(s) 391, 545
AoxI GGCC 2 cut(s) 201, 343
ApeKI GCWGC 2 cut(s) 702, 705
ApoI RAATTY 2 cut(s) 425, 460
AspLEI GCGC 1 cut(s) 719
AspS9I GGNCC 1 cut(s) 149
AsuHPI GGTGA 2 cut(s) 506, 714
AvaII GGWCC 1 cut(s) 149
BalI TGGCCA 1 cut(s) 203
BanI GGYRCC 2 cut(s) 624, 716
BanII GRGCYC 1 cut(s) 545
BbsI GAAGAC 1 cut(s) 420
Bbv12I GWGCWC 2 cut(s) 391, 545
BbvCI CCTCAGC 1 cut(s) 171
BbvI GCAGC 2 cut(s) 714, 717
BccI CCATC 3 cut(s) 184, 245, 271
BciT130I CCWGG 2 cut(s) 547, 657
BfaI CTAG 5 cut(s) 32, 153, 360, 375, 384
BfoI RGCGCY 1 cut(s) 720
BisI GCNGC 3 cut(s) 703, 706, 715
BlsI GCNGC 3 cut(s) 704, 707, 716
Bme1390I CCNGG 2 cut(s) 547, 657
Bme18I GGWCC 1 cut(s) 149
BmgT120I GGNCC 1 cut(s) 149
BmiI GGNNCC 3 cut(s) 117, 626, 718
BmrFI CCNGG 2 cut(s) 547, 657
BmsI GCATC 3 cut(s) 458, 522, 717
BoxI GACNNNNGTC 1 cut(s) 370
BpiI GAAGAC 1 cut(s) 420
BpmI CTGGAG 3 cut(s) 426, 529, 678
Bpu10I CCTNAGC 1 cut(s) 171
BpuEI CTTGAG 1 cut(s) 195
BsaHI GRCGYC 1 cut(s) 717
BsaJI CCNNGG 1 cut(s) 655
Bsc4I CCNNNNNNNGG 1 cut(s) 362
Bse1I ACTGG 2 cut(s) 89, 501
BseBI CCWGG 2 cut(s) 547, 657
BseDI CCNNGG 1 cut(s) 655
BseGI GGATG 1 cut(s) 253
BseLI CCNNNNNNNGG 1 cut(s) 362
BseMII CTCAG 5 cut(s) 185, 312, 408, 528, 691
BseNI ACTGG 2 cut(s) 89, 501
BseXI GCAGC 2 cut(s) 714, 717
Bsh1285I CGRYCG 1 cut(s) 520
BshFI GGCC 2 cut(s) 203, 345
BshNI GGYRCC 2 cut(s) 624, 716
BsiEI CGRYCG 1 cut(s) 520
BsiHKAI GWGCWC 2 cut(s) 391, 545
BslFI GGGAC 1 cut(s) 378
BslI CCNNNNNNNGG 1 cut(s) 362
BsmFI GGGAC 1 cut(s) 378
BsnI GGCC 2 cut(s) 203, 345
Bsp1286I GDGCHC 2 cut(s) 391, 545
Bsp143I GATC 1 cut(s) 274
BspACI CCGC 3 cut(s) 532, 685, 714
BspANI GGCC 2 cut(s) 203, 345
BspCNI CTCAG 5 cut(s) 184, 313, 407, 529, 690
BspHI TCATGA 1 cut(s) 160
BspLI GGNNCC 3 cut(s) 117, 626, 718
BspQI GCTCTTC 1 cut(s) 159
BspT107I GGYRCC 2 cut(s) 624, 716
BsrI ACTGG 2 cut(s) 89, 501
BssECI CCNNGG 1 cut(s) 655
BssMI GATC 1 cut(s) 274
BssNI GRCGYC 1 cut(s) 717
Bst2UI CCWGG 2 cut(s) 547, 657
Bst4CI ACNGT 1 cut(s) 281
Bst6I CTCTTC 2 cut(s) 159, 614
BstACI GRCGYC 1 cut(s) 717
BstAPI GCANNNNNTGC 1 cut(s) 455
BstC8I GCNNGC 2 cut(s) 29, 532
BstDEI CTNAG 7 cut(s) 171, 182, 237, 321, 394, 537, 677
BstF5I GGATG 1 cut(s) 253
BstH2I RGCGCY 1 cut(s) 720
BstHHI GCGC 1 cut(s) 719
BstKTI GATC 1 cut(s) 277
BstMBI GATC 1 cut(s) 274
BstMCI CGRYCG 1 cut(s) 520
BstMWI GCNNNNNNNGC 5 cut(s) 331, 455, 540, 711, 714
BstNI CCWGG 2 cut(s) 547, 657
BstNSI RCATGY 1 cut(s) 68
BstPAI GACNNNNGTC 1 cut(s) 370
BstSCI CCNGG 2 cut(s) 545, 655
BstV1I GCAGC 2 cut(s) 714, 717
BstV2I GAAGAC 1 cut(s) 420
BsuRI GGCC 2 cut(s) 203, 345
BtsCI GGATG 1 cut(s) 253
BtsI GCAGTG 1 cut(s) 651
BtsIMutI CAGTG 2 cut(s) 402, 651
Cac8I GCNNGC 2 cut(s) 29, 532
CciI TCATGA 1 cut(s) 160
CfoI GCGC 1 cut(s) 719
Cfr13I GGNCC 1 cut(s) 149
CseI GACGC 3 cut(s) 375, 425, 700
CviAII CATG 3 cut(s) 65, 161, 296
DdeI CTNAG 7 cut(s) 171, 182, 237, 321, 394, 537, 677
DinI GGCGCC 1 cut(s) 718
DpnI GATC 1 cut(s) 276
DpnII GATC 1 cut(s) 274
DraIII CACNNNGTG 1 cut(s) 397
EaeI YGGCCR 2 cut(s) 201, 343
Eam1104I CTCTTC 2 cut(s) 159, 614
EarI CTCTTC 2 cut(s) 159, 614
Ecl136II GAGCTC 1 cut(s) 543
Eco24I GRGCYC 1 cut(s) 545
Eco47I GGWCC 1 cut(s) 149
Eco53kI GAGCTC 1 cut(s) 543
Eco57I CTGAAG 1 cut(s) 624
EcoICRI GAGCTC 1 cut(s) 543
EcoO109I RGGNCCY 1 cut(s) 149
EcoRII CCWGG 2 cut(s) 545, 655
EcoT38I GRGCYC 1 cut(s) 545
EgeI GGCGCC 1 cut(s) 718
EheI GGCGCC 1 cut(s) 718
FaeI CATG 3 cut(s) 68, 164, 299
FaiI YATR 9 cut(s) 66, 101, 162, 221, 258, 297, 485, 615, 672
FaqI GGGAC 1 cut(s) 378
FatI CATG 3 cut(s) 64, 160, 295
FauI CCCGC 1 cut(s) 539
Fnu4HI GCNGC 3 cut(s) 703, 706, 715
FokI GGATG 1 cut(s) 260
FriOI GRGCYC 1 cut(s) 545
Fsp4HI GCNGC 3 cut(s) 703, 706, 715
FspBI CTAG 5 cut(s) 32, 153, 360, 375, 384
GlaI GCGC 1 cut(s) 718
GluI GCNGC 3 cut(s) 703, 706, 715
GsuI CTGGAG 3 cut(s) 426, 529, 678
HaeII RGCGCY 1 cut(s) 720
HaeIII GGCC 2 cut(s) 203, 345
HgaI GACGC 3 cut(s) 375, 425, 700
HhaI GCGC 1 cut(s) 719
Hin1I GRCGYC 1 cut(s) 717
Hin1II CATG 3 cut(s) 68, 164, 299
Hin6I GCGC 1 cut(s) 717
HinP1I GCGC 1 cut(s) 717
HinfI GANTC 2 cut(s) 317, 497
HphI GGTGA 2 cut(s) 506, 714
Hpy188I TCNGA 4 cut(s) 322, 331, 522, 680
Hpy188III TCNNGA 5 cut(s) 161, 311, 375, 384, 443
Hpy99I CGWCG 1 cut(s) 351
HpyAV CCTTC 2 cut(s) 394, 485
HpyCH4III ACNGT 1 cut(s) 281
HpyCH4IV ACGT 1 cut(s) 586
HpyCH4V TGCA 5 cut(s) 16, 27, 103, 458, 649
HpyF10VI GCNNNNNNNGC 5 cut(s) 331, 455, 540, 711, 714
HpyF3I CTNAG 7 cut(s) 171, 182, 237, 321, 394, 537, 677
HpySE526I ACGT 1 cut(s) 586
Hsp92I GRCGYC 1 cut(s) 717
Hsp92II CATG 3 cut(s) 68, 164, 299
HspAI GCGC 1 cut(s) 717
KasI GGCGCC 1 cut(s) 716
Kzo9I GATC 1 cut(s) 274
LguI GCTCTTC 1 cut(s) 159
LmnI GCTCC 2 cut(s) 265, 548
Lsp1109I GCAGC 2 cut(s) 714, 717
LweI GCATC 3 cut(s) 458, 522, 717
MaeI CTAG 5 cut(s) 32, 153, 360, 375, 384
MaeII ACGT 1 cut(s) 586
MaeIII GTNAC 3 cut(s) 46, 479, 598
MalI GATC 1 cut(s) 276
MboI GATC 1 cut(s) 274
MboII GAAGA 4 cut(s) 176, 326, 425, 631
MfeI CAATTG 1 cut(s) 267
MhlI GDGCHC 2 cut(s) 391, 545
MlsI TGGCCA 1 cut(s) 203
MluCI AATT 3 cut(s) 267, 425, 460
MluNI TGGCCA 1 cut(s) 203
Mly113I GGCGCC 1 cut(s) 717
MmeI TCCRAC 1 cut(s) 500
MnlI CCTC 6 cut(s) 130, 140, 180, 235, 615, 730
Mox20I TGGCCA 1 cut(s) 203
MscI TGGCCA 1 cut(s) 203
Msp20I TGGCCA 1 cut(s) 203
MspA1I CMGCKG 2 cut(s) 605, 714
MspR9I CCNGG 2 cut(s) 547, 657
MunI CAATTG 1 cut(s) 267
MvaI CCWGG 2 cut(s) 547, 657
MwoI GCNNNNNNNGC 5 cut(s) 331, 455, 540, 711, 714
NarI GGCGCC 1 cut(s) 717
NdeII GATC 1 cut(s) 274
NlaIII CATG 3 cut(s) 68, 164, 299
NlaIV GGNNCC 3 cut(s) 117, 626, 718
NmuCI GTSAC 1 cut(s) 598
NspI RCATGY 1 cut(s) 68
PagI TCATGA 1 cut(s) 160
PciI ACATGT 1 cut(s) 64
PciSI GCTCTTC 1 cut(s) 159
PfeI GAWTC 2 cut(s) 317, 497
PfoI TCCNGGA 1 cut(s) 545
PkrI GCNGC 3 cut(s) 704, 707, 716
PluTI GGCGCC 1 cut(s) 720
PpuMI RGGWCCY 1 cut(s) 149
PscI ACATGT 1 cut(s) 64
PshAI GACNNNNGTC 1 cut(s) 370
Psp124BI GAGCTC 1 cut(s) 545
Psp5II RGGWCCY 1 cut(s) 149
Psp6I CCWGG 2 cut(s) 545, 655
PspGI CCWGG 2 cut(s) 545, 655
PspN4I GGNNCC 3 cut(s) 117, 626, 718
PspPI GGNCC 1 cut(s) 149
PspPPI RGGWCCY 1 cut(s) 149
PvuII CAGCTG 1 cut(s) 605
SacI GAGCTC 1 cut(s) 545
SapI GCTCTTC 1 cut(s) 159
SatI GCNGC 3 cut(s) 703, 706, 715
Sau3AI GATC 1 cut(s) 274
Sau96I GGNCC 1 cut(s) 149
ScrFI CCNGG 2 cut(s) 547, 657
SduI GDGCHC 2 cut(s) 391, 545
SfaNI GCATC 3 cut(s) 458, 522, 717
SfoI GGCGCC 1 cut(s) 718
SinI GGWCC 1 cut(s) 149
SmlI CTYRAG 1 cut(s) 210
SmoI CTYRAG 1 cut(s) 210
Sse9I AATT 3 cut(s) 267, 425, 460
SsiI CCGC 3 cut(s) 532, 685, 714
SspDI GGCGCC 1 cut(s) 716
SspMI CTAG 5 cut(s) 32, 153, 360, 375, 384
SstI GAGCTC 1 cut(s) 545
StyD4I CCNGG 2 cut(s) 545, 655
TaaI ACNGT 1 cut(s) 281
TaiI ACGT 1 cut(s) 589
TaqII GACCGA 1 cut(s) 506
TasI AATT 3 cut(s) 267, 425, 460
TauI GCSGC 1 cut(s) 717
TfiI GAWTC 2 cut(s) 317, 497
TscAI CASTG 2 cut(s) 402, 651
TseFI GTSAC 1 cut(s) 598
TseI GCWGC 2 cut(s) 702, 705
Tsp45I GTSAC 1 cut(s) 598
TspDTI ATGAA 3 cut(s) 177, 274, 504
TspRI CASTG 2 cut(s) 402, 651
VpaK11BI GGWCC 1 cut(s) 149
XapI RAATTY 2 cut(s) 425, 460
XbaI TCTAGA 2 cut(s) 374, 383
XceI RCATGY 1 cut(s) 68
XspI CTAG 5 cut(s) 32, 153, 360, 375, 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.