Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr17

Orthogroup ID

OG0002457

Physical Location & Seq

Genomic Coordinates Forward (+)

149898 .. 151503

Gene Structure 1606 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom17g00220.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1101 bp

ATGAATGGGATTCTGACGTGCCAAAATCACGGCCACCAGCTAAATATAAATATATCGATAACACGGTCATTTCCCTATTGTTCCAAATTCCCATCACAAAGGGAAAATCTGGTTTGCCCATTTTATCCCTATCCTGCTCTCTTGTGCCCCGTCTTTCTTTACGGGCAGCATCTGTATAGCAGCGAAACCCGAAGGCCATTTCGCAGCGTAACAGTCGTAAACCTCAAAACTCAGAAGAATAGTTACAAAAAGAAGAAAAATAAAAAGGAAAACAATATGTTGAGGGTTGCGGCGAGGAGGCTCTGTTCTGTGTCCGTGTCGCCATGGAGGTCCAACCTAGCCGCCACCGGCACTGCCTCCTCCTCTATCCTGTCTCGAAATCTCATCGACGGCCATGACTCCTCCGATGAGCTCAGATCCGCCTATTTCACCCCTGACTTCTATCTTCCGTCCAGAGGTTTTGTGTCTGGTTCAGTTACCCCGTCCCAGGACAACAATCTAGTTCCAGACGTTCCTCCAACTGTCGCAGCTGTCAAGAATCCTAGTTCAAAGATAGTTTATGATGAGTACAACCATGAGCGTTACCCTCCAGGTGACCCCAGCAAGCGTGCATTTGCCTACTTTGTCCTCACAGGCGGGAGGTTTGTCTATGCTTCTCTGATTCGTCTGCTTATCCTCAAGTTTGTGCTAAGCATGTCAGCCAGTAAGGATGTTCTTGCTATGGCCTCGCTCGAGGTTGATCTCTCCAGCATTGAGCCCGGTTCAACTGTGACTGTTAAGTGGCGTGGAAAGCCAGTCTTCATCAGACGCCGCACTGACGATGATATCAAGCTGGCAAATAGTGTAGATGTTAATTCTCTCCGTGATCCGCAGCAAGATGCTGAGAGGGTTAAAAACCCGGAATGGCTCATTGTTGTGGGAGTTTGCACGCATCTGGGTTGCATTCCCTTGCCTAATGCTGGTGATTTTGGTGGATGGTTTTGTCCATGCCATGGTTCCCACTATGATATTTCTGGAAGGATCCGCAAGGGACCAGCACCATACAATCTGGAGGTACCCACTTATTCATTCTTGGAGGAGAACAAGTTACTGATTGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0016020 GO:0019866 GO:0031090 GO:0031966 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00190 ko01100 ko02020 ko04260 ko04714 ko04932 ko05010 ko05012 ko05016 map00190 map01100 map02020 map04260 map04714 map04932 map05010 map05012 map05016

Pfam Domains

Protein Families

Rieske UCR_TM

Protein Analysis

367

Amino Acids

40.9

Weight (kDa)

9.3

Isoelectric Point (pI)

55.69

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002457)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13430 AT5G13440
fragaria_vesca	FvH4_4g22600 FvH4_6g54320
malus_domestica	MD05G1033200.v1.1 MD05G1252000.v1.1 MD09G1002400.v1.1 MD13G1131100.v1.1 MD13G1261000.v1.1 MD16G1141300.v1.1 MD17G1006600.v1.1
prunus_persica	Prupe.1G214500_v2.0.a1 Prupe.3G313800_v2.0.a1
pyrus_communis	pycom111g00190 pycom13g11440 pycom16g11180 pycom17g00220
rosa_chinensis	RchiOBHm_Chr2g0176541 RchiOBHm_Chr4g0428841
rosa_laevigata	RLG00000007121 RLG00000022421
rosa_multiflora	Rmu_co8327683.1_g000001 Rmu_sc0001417.1_g000007 Rmu_sc0008612.1_g000002 Rmu_sc0009525.1_g000003 Rmu_sc0011904.1_g000004 Rmu_sc0013114.1_g000001
rosa_roxburghii	Rroxscaffold_2G00076320 Rroxscaffold_5G00370520
rosa_rugosa	Rorug02G0596000.1 Rorug04G0229600
rosa_samantha	Rh2AG673800 Rh2BG685000 Rh2CG648000 Rh2DG698900 Rh4AG286300 Rh4BG292100 Rh4CG308100 Rh4DG289300
rosa_wichuraiana	Rw2G055270 Rw4G024820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1054
AccB1I	GGYRCC	1 cut(s)	1054
AccB7I	CCANNNNNTGG	1 cut(s)	992
AciI	CCGC	7 cut(s)	290, 342, 420, 636, 811, 869, 1024
AclWI	GGATC	4 cut(s)	411, 860, 1015, 1028
AcoI	YGGCCR	2 cut(s)	31, 391
AcsI	RAATTY	1 cut(s)	86
AcyI	GRCGYC	1 cut(s)	808
AfaI	GTAC	2 cut(s)	569, 1056
AfiI	CCNNNNNNNGG	4 cut(s)	455, 487, 959, 992
AgsI	TTSAA	2 cut(s)	549, 765
AjiI	CACGTC	1 cut(s)	18
AjnI	CCWGG	2 cut(s)	486, 589
AloI	GAACNNNNNNTCC	2 cut(s)	289, 321
AluBI	AGCT	4 cut(s)	40, 412, 530, 832
AluI	AGCT	4 cut(s)	40, 412, 530, 832
Alw21I	GWGCWC	1 cut(s)	414
Alw26I	GTCTC	1 cut(s)	378
AlwI	GGATC	4 cut(s)	411, 860, 1015, 1028
AlwNI	CAGNNNCTG	1 cut(s)	172
Ama87I	CYCGRG	1 cut(s)	731
AoxI	GGCC	4 cut(s)	31, 194, 391, 723
ApeKI	GCWGC	5 cut(s)	166, 180, 204, 527, 871
ApoI	RAATTY	1 cut(s)	86
Asp718I	GGTACC	1 cut(s)	1054
AspS9I	GGNCC	2 cut(s)	330, 1031
AsuC2I	CCSGG	2 cut(s)	759, 899
AsuHPI	GGTGA	3 cut(s)	421, 605, 974
AvaI	CYCGRG	1 cut(s)	731
AvaII	GGWCC	2 cut(s)	330, 1031
BaeGI	GKGCMC	1 cut(s)	149
BamHI	GGATCC	1 cut(s)	1020
BanI	GGYRCC	1 cut(s)	1054
BanII	GRGCYC	2 cut(s)	414, 759
BarI	GAAGNNNNNNTAC	2 cut(s)	227, 259
BbsI	GAAGAC	1 cut(s)	790
Bbv12I	GWGCWC	1 cut(s)	414
BbvI	GCAGC	5 cut(s)	178, 192, 216, 539, 883
BccI	CCATC	2 cut(s)	100, 969
BceAI	ACGGC	2 cut(s)	46, 406
BciT130I	CCWGG	2 cut(s)	488, 591
BcnI	CCSGG	2 cut(s)	759, 899
BcoDI	GTCTC	1 cut(s)	378
BfaI	CTAG	3 cut(s)	338, 500, 543
BisI	GCNGC	8 cut(s)	167, 181, 205, 291, 342, 528, 811, 872
BlpI	GCTNAGC	1 cut(s)	689
BlsI	GCNGC	8 cut(s)	168, 182, 206, 292, 343, 529, 812, 873
Bme1390I	CCNGG	4 cut(s)	488, 591, 759, 899
Bme18I	GGWCC	2 cut(s)	330, 1031
BmeT110I	CYCGRG	1 cut(s)	731
BmgBI	CACGTC	1 cut(s)	18
BmgT120I	GGNCC	2 cut(s)	330, 1031
BmiI	GGNNCC	4 cut(s)	997, 1022, 1032, 1056
BmrFI	CCNGG	4 cut(s)	488, 591, 759, 899
BmsI	GCATC	3 cut(s)	178, 868, 940
BpiI	GAAGAC	1 cut(s)	790
BpmI	CTGGAG	3 cut(s)	573, 730, 1070
Bpu1102I	GCTNAGC	1 cut(s)	689
BpuEI	CTTGAG	1 cut(s)	662
BpuMI	CCSGG	2 cut(s)	759, 899
Bsa29I	ATCGAT	1 cut(s)	56
BsaHI	GRCGYC	1 cut(s)	808
BsaJI	CCNNGG	3 cut(s)	323, 486, 991
BsaXI	ACNNNNNCTCC	4 cut(s)	289, 319, 1070, 1100
Bsc4I	CCNNNNNNNGG	4 cut(s)	455, 487, 959, 992
Bse118I	RCCGGY	1 cut(s)	347
Bse1I	ACTGG	2 cut(s)	702, 794
BseBI	CCWGG	2 cut(s)	488, 591
BseCI	ATCGAT	1 cut(s)	56
BseDI	CCNNGG	3 cut(s)	323, 486, 991
BseGI	GGATG	2 cut(s)	715, 980
BseLI	CCNNNNNNNGG	4 cut(s)	455, 487, 959, 992
BseMII	CTCAG	3 cut(s)	245, 427, 873
BseNI	ACTGG	2 cut(s)	702, 794
BseRI	GAGGAG	5 cut(s)	310, 349, 352, 391, 1091
BseSI	GKGCMC	1 cut(s)	149
BseXI	GCAGC	5 cut(s)	178, 192, 216, 539, 883
BseYI	CCCAGC	1 cut(s)	599
BshFI	GGCC	4 cut(s)	33, 196, 393, 725
BshNI	GGYRCC	1 cut(s)	1054
BshVI	ATCGAT	1 cut(s)	56
BsiHKAI	GWGCWC	1 cut(s)	414
BsiHKCI	CYCGRG	1 cut(s)	731
BsiSI	CCGG	3 cut(s)	348, 759, 899
BslFI	GGGAC	2 cut(s)	469, 1044
BslI	CCNNNNNNNGG	4 cut(s)	455, 487, 959, 992
BsmAI	GTCTC	1 cut(s)	378
BsmFI	GGGAC	2 cut(s)	469, 1044
BsmI	GAATGC	1 cut(s)	942
BsnI	GGCC	4 cut(s)	33, 196, 393, 725
BsoBI	CYCGRG	1 cut(s)	731
Bsp1286I	GDGCHC	3 cut(s)	149, 414, 759
Bsp143I	GATC	4 cut(s)	416, 739, 865, 1020
Bsp1720I	GCTNAGC	1 cut(s)	689
Bsp19I	CCATGG	2 cut(s)	323, 991
BspACI	CCGC	7 cut(s)	290, 342, 420, 636, 811, 869, 1024
BspANI	GGCC	4 cut(s)	33, 196, 393, 725
BspCNI	CTCAG	3 cut(s)	244, 426, 874
BspDI	ATCGAT	1 cut(s)	56
BspLI	GGNNCC	4 cut(s)	997, 1022, 1032, 1056
BspPI	GGATC	4 cut(s)	411, 860, 1015, 1028
BspT107I	GGYRCC	1 cut(s)	1054
BsrFI	RCCGGY	1 cut(s)	347
BsrI	ACTGG	2 cut(s)	702, 794
BssAI	RCCGGY	1 cut(s)	347
BssECI	CCNNGG	3 cut(s)	323, 486, 991
BssMI	GATC	4 cut(s)	416, 739, 865, 1020
BssNI	GRCGYC	1 cut(s)	808
BssT1I	CCWWGG	2 cut(s)	323, 991
Bst2UI	CCWGG	2 cut(s)	488, 591
Bst4CI	ACNGT	5 cut(s)	66, 214, 523, 769, 775
BstACI	GRCGYC	1 cut(s)	808
BstC8I	GCNNGC	4 cut(s)	605, 609, 834, 929
BstDEI	CTNAG	4 cut(s)	231, 413, 689, 882
BstDSI	CCRYGG	2 cut(s)	323, 991
BstEII	GGTNACC	1 cut(s)	593
BstF5I	GGATG	2 cut(s)	715, 980
BstKTI	GATC	4 cut(s)	419, 742, 868, 1023
BstMAI	GTCTC	1 cut(s)	378
BstMBI	GATC	4 cut(s)	416, 739, 865, 1020
BstMWI	GCNNNNNNNGC	1 cut(s)	790
BstNI	CCWGG	2 cut(s)	488, 591
BstNSI	RCATGY	1 cut(s)	697
BstPI	GGTNACC	1 cut(s)	593
BstSCI	CCNGG	4 cut(s)	486, 589, 757, 897
BstSLI	GKGCMC	1 cut(s)	149
BstV1I	GCAGC	5 cut(s)	178, 192, 216, 539, 883
BstV2I	GAAGAC	1 cut(s)	790
BstX2I	RGATCY	2 cut(s)	416, 1020
BstYI	RGATCY	2 cut(s)	416, 1020
Bsu15I	ATCGAT	1 cut(s)	56
BsuRI	GGCC	4 cut(s)	33, 196, 393, 725
BsuTUI	ATCGAT	1 cut(s)	56
BtgI	CCRYGG	2 cut(s)	323, 991
BtrI	CACGTC	1 cut(s)	18
BtsCI	GGATG	2 cut(s)	715, 980
BtsI	GCAGTG	1 cut(s)	351
BtsIMutI	CAGTG	2 cut(s)	351, 813
Cac8I	GCNNGC	4 cut(s)	605, 609, 834, 929
CaiI	CAGNNNCTG	1 cut(s)	172
Cfr10I	RCCGGY	1 cut(s)	347
Cfr13I	GGNCC	2 cut(s)	330, 1031
ClaI	ATCGAT	1 cut(s)	56
CseI	GACGC	1 cut(s)	816
Csp6I	GTAC	2 cut(s)	568, 1055
CviAII	CATG	6 cut(s)	324, 395, 575, 694, 987, 992
CviQI	GTAC	2 cut(s)	568, 1055
DdeI	CTNAG	4 cut(s)	231, 413, 689, 882
DpnI	GATC	4 cut(s)	418, 741, 867, 1022
DpnII	GATC	4 cut(s)	416, 739, 865, 1020
EaeI	YGGCCR	2 cut(s)	31, 391
EciI	GGCGGA	1 cut(s)	409
Ecl136II	GAGCTC	1 cut(s)	412
Eco130I	CCWWGG	2 cut(s)	323, 991
Eco24I	GRGCYC	2 cut(s)	414, 759
Eco32I	GATATC	1 cut(s)	826
Eco47I	GGWCC	2 cut(s)	330, 1031
Eco53kI	GAGCTC	1 cut(s)	412
Eco88I	CYCGRG	1 cut(s)	731
Eco91I	GGTNACC	1 cut(s)	593
EcoICRI	GAGCTC	1 cut(s)	412
EcoO65I	GGTNACC	1 cut(s)	593
EcoRII	CCWGG	2 cut(s)	486, 589
EcoRV	GATATC	1 cut(s)	826
EcoT14I	CCWWGG	2 cut(s)	323, 991
EcoT38I	GRGCYC	2 cut(s)	414, 759
ErhI	CCWWGG	2 cut(s)	323, 991
FaeI	CATG	6 cut(s)	327, 398, 578, 697, 990, 995
FalI	AAGNNNNNCTT	2 cut(s)	782, 814
FaqI	GGGAC	2 cut(s)	469, 1044
FatI	CATG	6 cut(s)	323, 394, 574, 693, 986, 991
FauI	CCCGC	1 cut(s)	629
Fnu4HI	GCNGC	8 cut(s)	167, 181, 205, 291, 342, 528, 811, 872
FokI	GGATG	2 cut(s)	722, 987
FriOI	GRGCYC	2 cut(s)	414, 759
Fsp4HI	GCNGC	8 cut(s)	167, 181, 205, 291, 342, 528, 811, 872
FspBI	CTAG	3 cut(s)	338, 500, 543
GluI	GCNGC	8 cut(s)	167, 181, 205, 291, 342, 528, 811, 872
GsaI	CCCAGC	1 cut(s)	603
GsuI	CTGGAG	3 cut(s)	573, 730, 1070
HaeIII	GGCC	4 cut(s)	33, 196, 393, 725
HapII	CCGG	3 cut(s)	348, 759, 899
HgaI	GACGC	1 cut(s)	816
Hin1I	GRCGYC	1 cut(s)	808
Hin1II	CATG	6 cut(s)	327, 398, 578, 697, 990, 995
HinfI	GANTC	4 cut(s)	10, 398, 538, 661
HpaII	CCGG	3 cut(s)	348, 759, 899
HphI	GGTGA	3 cut(s)	421, 605, 974
Hpy166II	GTNNAC	1 cut(s)	220
Hpy188I	TCNGA	6 cut(s)	15, 234, 406, 416, 660, 806
Hpy188III	TCNNGA	6 cut(s)	375, 453, 506, 535, 1014, 1049
Hpy8I	GTNNAC	1 cut(s)	220
Hpy99I	CGWCG	1 cut(s)	392
HpyAV	CCTTC	2 cut(s)	186, 1011
HpyCH4III	ACNGT	5 cut(s)	66, 214, 523, 769, 775
HpyCH4IV	ACGT	2 cut(s)	17, 510
HpyCH4V	TGCA	3 cut(s)	611, 927, 942
HpyF10VI	GCNNNNNNNGC	1 cut(s)	790
HpyF3I	CTNAG	4 cut(s)	231, 413, 689, 882
HpySE526I	ACGT	2 cut(s)	17, 510
Hsp92I	GRCGYC	1 cut(s)	808
Hsp92II	CATG	6 cut(s)	327, 398, 578, 697, 990, 995
KpnI	GGTACC	1 cut(s)	1058
Kzo9I	GATC	4 cut(s)	416, 739, 865, 1020
Lsp1109I	GCAGC	5 cut(s)	178, 192, 216, 539, 883
LweI	GCATC	3 cut(s)	178, 868, 940
MaeI	CTAG	3 cut(s)	338, 500, 543
MaeII	ACGT	2 cut(s)	17, 510
MaeIII	GTNAC	7 cut(s)	208, 242, 475, 581, 593, 769, 1086
MalI	GATC	4 cut(s)	418, 741, 867, 1022
MboI	GATC	4 cut(s)	416, 739, 865, 1020
MboII	GAAGA	4 cut(s)	247, 265, 437, 790
MflI	RGATCY	2 cut(s)	416, 1020
MhlI	GDGCHC	3 cut(s)	149, 414, 759
MluCI	AATT	2 cut(s)	86, 853
MlyI	GAGTC	1 cut(s)	392
MmeI	TCCRAC	2 cut(s)	357, 542
MseI	TTAA	3 cut(s)	777, 852, 891
MslI	CAYNNNNRTG	1 cut(s)	914
MspA1I	CMGCKG	1 cut(s)	530
MspI	CCGG	3 cut(s)	348, 759, 899
MspR9I	CCNGG	4 cut(s)	488, 591, 759, 899
Mva1269I	GAATGC	1 cut(s)	942
MvaI	CCWGG	2 cut(s)	488, 591
MwoI	GCNNNNNNNGC	1 cut(s)	790
NciI	CCSGG	2 cut(s)	759, 899
NcoI	CCATGG	2 cut(s)	323, 991
NdeII	GATC	4 cut(s)	416, 739, 865, 1020
NlaIII	CATG	6 cut(s)	327, 398, 578, 697, 990, 995
NlaIV	GGNNCC	4 cut(s)	997, 1022, 1032, 1056
NmuCI	GTSAC	2 cut(s)	593, 769
NspI	RCATGY	1 cut(s)	697
PaeR7I	CTCGAG	1 cut(s)	731
PctI	GAATGC	1 cut(s)	942
PfeI	GAWTC	3 cut(s)	10, 538, 661
PflMI	CCANNNNNTGG	1 cut(s)	992
PkrI	GCNGC	8 cut(s)	168, 182, 206, 292, 343, 529, 812, 873
PleI	GAGTC	1 cut(s)	392
PpsI	GAGTC	1 cut(s)	392
Psp124BI	GAGCTC	1 cut(s)	414
Psp6I	CCWGG	2 cut(s)	486, 589
PspEI	GGTNACC	1 cut(s)	593
PspFI	CCCAGC	1 cut(s)	599
PspGI	CCWGG	2 cut(s)	486, 589
PspN4I	GGNNCC	4 cut(s)	997, 1022, 1032, 1056
PspPI	GGNCC	2 cut(s)	330, 1031
PspXI	VCTCGAGB	1 cut(s)	731
PstNI	CAGNNNCTG	1 cut(s)	172
PsuI	RGATCY	2 cut(s)	416, 1020
PvuII	CAGCTG	1 cut(s)	530
RsaI	GTAC	2 cut(s)	569, 1056
RsaNI	GTAC	2 cut(s)	568, 1055
RseI	CAYNNNNRTG	1 cut(s)	914
SacI	GAGCTC	1 cut(s)	414
SaqAI	TTAA	3 cut(s)	777, 852, 891
SatI	GCNGC	8 cut(s)	167, 181, 205, 291, 342, 528, 811, 872
Sau3AI	GATC	4 cut(s)	416, 739, 865, 1020
Sau96I	GGNCC	2 cut(s)	330, 1031
SchI	GAGTC	1 cut(s)	392
ScrFI	CCNGG	4 cut(s)	488, 591, 759, 899
SduI	GDGCHC	3 cut(s)	149, 414, 759
SfaNI	GCATC	3 cut(s)	178, 868, 940
Sfr274I	CTCGAG	1 cut(s)	731
SinI	GGWCC	2 cut(s)	330, 1031
SlaI	CTCGAG	1 cut(s)	731
SmiMI	CAYNNNNRTG	1 cut(s)	914
SmlI	CTYRAG	2 cut(s)	677, 731
SmoI	CTYRAG	2 cut(s)	677, 731
Sse9I	AATT	2 cut(s)	86, 853
SsiI	CCGC	7 cut(s)	290, 342, 420, 636, 811, 869, 1024
SspMI	CTAG	3 cut(s)	338, 500, 543
SstI	GAGCTC	1 cut(s)	414
StyD4I	CCNGG	4 cut(s)	486, 589, 757, 897
StyI	CCWWGG	2 cut(s)	323, 991
TaaI	ACNGT	5 cut(s)	66, 214, 523, 769, 775
TaiI	ACGT	2 cut(s)	20, 513
TaqI	TCGA	4 cut(s)	56, 376, 387, 732
TasI	AATT	2 cut(s)	86, 853
TatI	WGTACW	1 cut(s)	567
TauI	GCSGC	3 cut(s)	293, 344, 813
TfiI	GAWTC	3 cut(s)	10, 538, 661
Tru1I	TTAA	3 cut(s)	777, 852, 891
Tru9I	TTAA	3 cut(s)	777, 852, 891
TscAI	CASTG	2 cut(s)	358, 820
TseFI	GTSAC	2 cut(s)	593, 769
TseI	GCWGC	5 cut(s)	166, 180, 204, 527, 871
Tsp45I	GTSAC	2 cut(s)	593, 769
TspDTI	ATGAA	3 cut(s)	17, 790, 1056
TspGWI	ACGGA	3 cut(s)	304, 438, 851
TspRI	CASTG	2 cut(s)	358, 820
Van91I	CCANNNNNTGG	1 cut(s)	992
VpaK11BI	GGWCC	2 cut(s)	330, 1031
XapI	RAATTY	1 cut(s)	86
XceI	RCATGY	1 cut(s)	697
XhoI	CTCGAG	1 cut(s)	731
XspI	CTAG	3 cut(s)	338, 500, 543

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.