pycom17g06500

tRNA (Guanine(26)-N(2))-dimethyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0001945
Physical Location & Seq
Reverse (-)
4674939 .. 4677480
2542 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1053 bp
ATGTGTACAGCAACTGATATGGCAGTGCTATGTGGTGGAAATGGGGAGGTTTGCTATTCAAAATATGGTTCCTATCCATTGAGAGGGAAGTATTGTCACGAAATGGCTTTGAGGATCCTCCTAGCTTGCATTGAGAGTCATGCAAATCGTTACAAACGGTACATTGTTCCTGTGCTATCTGTTCAGATGGACTTTTATGTTCGAGTTTTCGTCCGTATCTACTCTTCTGCAAGTGCAATGAAGAACTCTCCCCTTAAGCTATCCTATGTTTATCAGTGCATTGGCTGCGATTCATTTCATCTTCAGCCTATTGGAAGGACAGTCACAAAGAACACCAGTGTAAGATATCTACCGGGTTTTGGTCCCGTTGTTCCTCAAGAATGCAGTGACTGTGGGAAGAAATTCAATATGGGTGGACCTATATGGTCTGCTCCTATCCATGATCAAGAATGGGTGACTTCCATACTATCTGATGTGAAATCTATGAAGGAGCGGTATCCCGCTTATGGTCGCATCTCTGCTGTACTAACAACAATTTCAGAGGAACTGCCTGATGTTCCTTTGTTCTTGAGTCTGCACAACCTCTGTGCCACATTAAAGTGCACTTCCCCATCTGCTGTGATTTTCCGTTCTGCTGTGATCAATGCAGGGTATCGTATATCTGGAACTCACGTAAACCCATTGGGGTTGAAATCAGATGCTCCCATGGATGTCATTTGGGACATAATGCGCTGTTGGGTGAAGAATCACCCGGTGAAACCTCAGCCAGCGGATCAGTCAGGAACCGTGATACTTGCCAAGGAACCCATCCTTCAAGCTAACTTTGCTCGAGCTGTTGCGTCTCTTAGCAAGGCACAAACCAAGAAGGTAGCACGCTTCCTTCCAAATCCAGAAAGGCACTGGGGGCCAAAGCTTAGGGCAGGTCGTACAATCACCAGCAAACACATCTCTCTCTTGGGTCCGGAGGCTGTCAATGGACATCTTAACCATGAAGATGGTGAGGAGCATGATGCCAAGCGTCAAAAGATTGAAGATCAACCCGCCCCGAAGTGA

Protein Analysis

351

Amino Acids

38.84

Weight (kDa)

9.17

Isoelectric Point (pI)

45.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRM PF02005 2 - 244 3.8e-70 N2,N2-dimethylguanosine tRNA methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 911
AccBSI CCGCTC 1 cut(s) 493
AccIII TCCGGA 1 cut(s) 961
AciI CCGC 4 cut(s) 493, 501, 770, 1041
AclWI GGATC 3 cut(s) 109, 122, 780
AcsI RAATTY 1 cut(s) 401
AcuI CTGAAG 1 cut(s) 287
AdeI CACNNNGTG 1 cut(s) 754
AfaI GTAC 4 cut(s) 7, 161, 525, 928
AfiI CCNNNNNNNGG 3 cut(s) 83, 359, 506
AflII CTTAAG 1 cut(s) 254
AgsI TTSAA 5 cut(s) 60, 406, 691, 815, 1031
AluBI AGCT 5 cut(s) 125, 259, 818, 833, 913
AluI AGCT 5 cut(s) 125, 259, 818, 833, 913
Alw21I GWGCWC 1 cut(s) 605
Alw26I GTCTC 1 cut(s) 846
Alw44I GTGCAC 1 cut(s) 601
AlwI GGATC 3 cut(s) 109, 122, 780
AlwNI CAGNNNCTG 2 cut(s) 14, 390
Ama87I CYCGRG 1 cut(s) 828
Aor13HI TCCGGA 1 cut(s) 961
AoxI GGCC 1 cut(s) 905
ApaLI GTGCAC 1 cut(s) 601
ApeKI GCWGC 1 cut(s) 285
ApoI RAATTY 1 cut(s) 401
Asp700I GAANNNNTTC 1 cut(s) 401
AspLEI GCGC 1 cut(s) 732
AspS9I GGNCC 4 cut(s) 362, 416, 905, 959
AsuC2I CCSGG 2 cut(s) 354, 752
AsuHPI GGTGA 6 cut(s) 466, 740, 751, 766, 925, 1010
AvaI CYCGRG 1 cut(s) 828
AvaII GGWCC 3 cut(s) 362, 416, 959
BaeGI GKGCMC 1 cut(s) 605
BamHI GGATCC 1 cut(s) 114
BarI GAAGNNNNNNTAC 2 cut(s) 208, 240
Bbv12I GWGCWC 1 cut(s) 605
BbvCI CCTCAGC 1 cut(s) 762
BbvI GCAGC 1 cut(s) 272
BccI CCATC 4 cut(s) 181, 619, 815, 989
BciVI GTATCC 1 cut(s) 507
BclI TGATCA 2 cut(s) 442, 639
BcnI CCSGG 2 cut(s) 354, 752
BcoDI GTCTC 1 cut(s) 846
BfaI CTAG 1 cut(s) 122
BfrI CTTAAG 1 cut(s) 254
BfuAI ACCTGC 1 cut(s) 911
BfuI GTATCC 1 cut(s) 507
BisI GCNGC 1 cut(s) 286
BlsI GCNGC 1 cut(s) 287
Bme1390I CCNGG 2 cut(s) 354, 752
Bme18I GGWCC 3 cut(s) 362, 416, 959
BmeT110I CYCGRG 1 cut(s) 828
BmgT120I GGNCC 4 cut(s) 362, 416, 905, 959
BmiI GGNNCC 7 cut(s) 70, 116, 364, 784, 804, 906, 960
BmrFI CCNGG 2 cut(s) 354, 752
BmrI ACTGGG 1 cut(s) 910
BmsI GCATC 3 cut(s) 522, 688, 1000
BmuI ACTGGG 1 cut(s) 910
Bpu10I CCTNAGC 2 cut(s) 762, 914
BpuEI CTTGAG 2 cut(s) 360, 589
BpuMI CCSGG 2 cut(s) 354, 752
BsaAI YACGTR 1 cut(s) 673
BsaJI CCNNGG 2 cut(s) 705, 798
BsaWI WCCGGW 1 cut(s) 961
Bsc4I CCNNNNNNNGG 3 cut(s) 83, 359, 506
Bse1I ACTGG 2 cut(s) 336, 905
Bse3DI GCAATG 1 cut(s) 243
BseAI TCCGGA 1 cut(s) 961
BseDI CCNNGG 2 cut(s) 705, 798
BseGI GGATG 2 cut(s) 715, 807
BseLI CCNNNNNNNGG 3 cut(s) 83, 359, 506
BseMI GCAATG 1 cut(s) 243
BseMII CTCAG 1 cut(s) 776
BseNI ACTGG 2 cut(s) 336, 905
BseRI GAGGAG 1 cut(s) 1016
BseSI GKGCMC 1 cut(s) 605
BseXI GCAGC 1 cut(s) 272
BsgI GTGCAG 1 cut(s) 560
BshFI GGCC 1 cut(s) 907
BsiHKAI GWGCWC 1 cut(s) 605
BsiHKCI CYCGRG 1 cut(s) 828
BsiSI CCGG 3 cut(s) 353, 752, 962
BslFI GGGAC 2 cut(s) 348, 734
BslI CCNNNNNNNGG 3 cut(s) 83, 359, 506
BsmAI GTCTC 1 cut(s) 846
BsmBI CGTCTC 1 cut(s) 846
BsmFI GGGAC 2 cut(s) 348, 734
BsmI GAATGC 1 cut(s) 386
BsnI GGCC 1 cut(s) 907
BsoBI CYCGRG 1 cut(s) 828
Bsp1286I GDGCHC 1 cut(s) 605
Bsp13I TCCGGA 1 cut(s) 961
Bsp1407I TGTACA 1 cut(s) 5
Bsp143I GATC 5 cut(s) 114, 442, 639, 772, 1033
Bsp19I CCATGG 1 cut(s) 705
BspACI CCGC 4 cut(s) 493, 501, 770, 1041
BspANI GGCC 1 cut(s) 907
BspCNI CTCAG 1 cut(s) 775
BspEI TCCGGA 1 cut(s) 961
BspLI GGNNCC 7 cut(s) 70, 116, 364, 784, 804, 906, 960
BspMI ACCTGC 1 cut(s) 911
BspPI GGATC 3 cut(s) 109, 122, 780
BspTI CTTAAG 1 cut(s) 254
BsrBI CCGCTC 1 cut(s) 493
BsrDI GCAATG 1 cut(s) 243
BsrGI TGTACA 1 cut(s) 5
BsrI ACTGG 2 cut(s) 336, 905
BssECI CCNNGG 2 cut(s) 705, 798
BssMI GATC 5 cut(s) 114, 442, 639, 772, 1033
BssT1I CCWWGG 2 cut(s) 705, 798
Bst4CI ACNGT 4 cut(s) 159, 322, 392, 787
Bst6I CTCTTC 1 cut(s) 229
BstAFI CTTAAG 1 cut(s) 254
BstAPI GCANNNNNTGC 1 cut(s) 285
BstAUI TGTACA 1 cut(s) 5
BstBAI YACGTR 1 cut(s) 673
BstC8I GCNNGC 3 cut(s) 127, 768, 874
BstDEI CTNAG 3 cut(s) 762, 845, 914
BstDSI CCRYGG 1 cut(s) 705
BstF5I GGATG 2 cut(s) 715, 807
BstHHI GCGC 1 cut(s) 732
BstKTI GATC 5 cut(s) 117, 445, 642, 775, 1036
BstMAI GTCTC 1 cut(s) 846
BstMBI GATC 5 cut(s) 114, 442, 639, 772, 1033
BstMWI GCNNNNNNNGC 3 cut(s) 285, 824, 904
BstSCI CCNGG 2 cut(s) 352, 750
BstSLI GKGCMC 1 cut(s) 605
BstV1I GCAGC 1 cut(s) 272
BstX2I RGATCY 1 cut(s) 114
BstXI CCANNNNNNTGG 1 cut(s) 995
BstYI RGATCY 1 cut(s) 114
BsuI GTATCC 1 cut(s) 507
BsuRI GGCC 1 cut(s) 907
BtgI CCRYGG 1 cut(s) 705
BtsCI GGATG 2 cut(s) 715, 807
BtsI GCAGTG 2 cut(s) 30, 391
BtsIMutI CAGTG 5 cut(s) 30, 281, 343, 391, 898
BveI ACCTGC 1 cut(s) 911
Cac8I GCNNGC 3 cut(s) 127, 768, 874
CaiI CAGNNNCTG 2 cut(s) 14, 390
CfoI GCGC 1 cut(s) 732
Cfr13I GGNCC 4 cut(s) 362, 416, 905, 959
CseI GACGC 2 cut(s) 828, 1007
Csp6I GTAC 4 cut(s) 6, 160, 524, 927
CspCI CAANNNNNGTGG 2 cut(s) 394, 429
CviAII CATG 5 cut(s) 140, 440, 706, 989, 1007
CviQI GTAC 4 cut(s) 6, 160, 524, 927
DdeI CTNAG 3 cut(s) 762, 845, 914
DpnI GATC 5 cut(s) 116, 444, 641, 774, 1035
DpnII GATC 5 cut(s) 114, 442, 639, 772, 1033
DraIII CACNNNGTG 1 cut(s) 754
Eam1104I CTCTTC 1 cut(s) 229
EarI CTCTTC 1 cut(s) 229
Eco130I CCWWGG 2 cut(s) 705, 798
Eco32I GATATC 1 cut(s) 347
Eco47I GGWCC 3 cut(s) 362, 416, 959
Eco57I CTGAAG 1 cut(s) 287
Eco88I CYCGRG 1 cut(s) 828
EcoRV GATATC 1 cut(s) 347
EcoT14I CCWWGG 2 cut(s) 705, 798
ErhI CCWWGG 2 cut(s) 705, 798
Esp3I CGTCTC 1 cut(s) 846
FaeI CATG 5 cut(s) 143, 443, 709, 992, 1010
FaqI GGGAC 2 cut(s) 348, 734
FatI CATG 5 cut(s) 139, 439, 705, 988, 1006
FauI CCCGC 2 cut(s) 508, 1048
FbaI TGATCA 2 cut(s) 442, 639
Fnu4HI GCNGC 1 cut(s) 286
FokI GGATG 2 cut(s) 722, 794
Fsp4HI GCNGC 1 cut(s) 286
FspBI CTAG 1 cut(s) 122
GlaI GCGC 1 cut(s) 731
GluI GCNGC 1 cut(s) 286
HaeIII GGCC 1 cut(s) 907
HapII CCGG 3 cut(s) 353, 752, 962
HgaI GACGC 2 cut(s) 828, 1007
HhaI GCGC 1 cut(s) 732
Hin1II CATG 5 cut(s) 143, 443, 709, 992, 1010
Hin6I GCGC 1 cut(s) 730
HinP1I GCGC 1 cut(s) 730
HindIII AAGCTT 1 cut(s) 911
HinfI GANTC 4 cut(s) 136, 290, 571, 745
HpaII CCGG 3 cut(s) 353, 752, 962
HphI GGTGA 6 cut(s) 466, 740, 751, 766, 925, 1010
Hpy166II GTNNAC 4 cut(s) 6, 416, 603, 676
Hpy188I TCNGA 4 cut(s) 186, 472, 541, 697
Hpy188III TCNNGA 8 cut(s) 98, 377, 446, 568, 663, 780, 890, 962
Hpy8I GTNNAC 4 cut(s) 6, 416, 603, 676
HpyAV CCTTC 5 cut(s) 309, 481, 821, 859, 890
HpyCH4III ACNGT 4 cut(s) 159, 322, 392, 787
HpyCH4IV ACGT 1 cut(s) 672
HpyCH4V TGCA 9 cut(s) 129, 143, 230, 236, 279, 384, 577, 603, 647
HpyF10VI GCNNNNNNNGC 3 cut(s) 285, 824, 904
HpyF3I CTNAG 3 cut(s) 762, 845, 914
HpySE526I ACGT 1 cut(s) 672
Hsp92II CATG 5 cut(s) 143, 443, 709, 992, 1010
HspAI GCGC 1 cut(s) 730
Kpn2I TCCGGA 1 cut(s) 961
Ksp22I TGATCA 2 cut(s) 442, 639
Kzo9I GATC 5 cut(s) 114, 442, 639, 772, 1033
LmnI GCTCC 4 cut(s) 436, 490, 706, 1003
Lsp1109I GCAGC 1 cut(s) 272
LweI GCATC 3 cut(s) 522, 688, 1000
MaeI CTAG 1 cut(s) 122
MaeII ACGT 1 cut(s) 672
MaeIII GTNAC 5 cut(s) 95, 149, 322, 386, 454
MalI GATC 5 cut(s) 116, 444, 641, 774, 1035
MbiI CCGCTC 1 cut(s) 493
MboI GATC 5 cut(s) 114, 442, 639, 772, 1033
MboII GAAGA 7 cut(s) 216, 253, 293, 409, 754, 1004, 1043
MflI RGATCY 1 cut(s) 114
MhlI GDGCHC 1 cut(s) 605
MluCI AATT 2 cut(s) 401, 534
MlyI GAGTC 2 cut(s) 145, 580
MroI TCCGGA 1 cut(s) 961
MroXI GAANNNNTTC 1 cut(s) 401
MseI TTAA 3 cut(s) 255, 596, 984
MslI CAYNNNNRTG 2 cut(s) 598, 993
MspA1I CMGCKG 1 cut(s) 770
MspCI CTTAAG 1 cut(s) 254
MspI CCGG 3 cut(s) 353, 752, 962
MspR9I CCNGG 2 cut(s) 354, 752
Mva1269I GAATGC 1 cut(s) 386
MwoI GCNNNNNNNGC 3 cut(s) 285, 824, 904
NciI CCSGG 2 cut(s) 354, 752
NcoI CCATGG 1 cut(s) 705
NdeII GATC 5 cut(s) 114, 442, 639, 772, 1033
NlaIII CATG 5 cut(s) 143, 443, 709, 992, 1010
NlaIV GGNNCC 7 cut(s) 70, 116, 364, 784, 804, 906, 960
NmuCI GTSAC 4 cut(s) 95, 322, 386, 454
PaeR7I CTCGAG 1 cut(s) 828
PctI GAATGC 1 cut(s) 386
PdmI GAANNNNTTC 1 cut(s) 401
PfeI GAWTC 2 cut(s) 290, 745
PkrI GCNGC 1 cut(s) 287
PleI GAGTC 2 cut(s) 144, 579
PpsI GAGTC 2 cut(s) 144, 579
Ppu21I YACGTR 1 cut(s) 673
PspN4I GGNNCC 7 cut(s) 70, 116, 364, 784, 804, 906, 960
PspPI GGNCC 4 cut(s) 362, 416, 905, 959
PspXI VCTCGAGB 1 cut(s) 828
PstNI CAGNNNCTG 2 cut(s) 14, 390
PsuI RGATCY 1 cut(s) 114
RsaI GTAC 4 cut(s) 7, 161, 525, 928
RsaNI GTAC 4 cut(s) 6, 160, 524, 927
RseI CAYNNNNRTG 2 cut(s) 598, 993
SaqAI TTAA 3 cut(s) 255, 596, 984
SatI GCNGC 1 cut(s) 286
Sau3AI GATC 5 cut(s) 114, 442, 639, 772, 1033
Sau96I GGNCC 4 cut(s) 362, 416, 905, 959
SchI GAGTC 2 cut(s) 145, 580
ScrFI CCNGG 2 cut(s) 354, 752
SduI GDGCHC 1 cut(s) 605
SfaNI GCATC 3 cut(s) 522, 688, 1000
Sfr274I CTCGAG 1 cut(s) 828
SinI GGWCC 3 cut(s) 362, 416, 959
SlaI CTCGAG 1 cut(s) 828
SmiMI CAYNNNNRTG 2 cut(s) 598, 993
SmlI CTYRAG 4 cut(s) 254, 375, 568, 828
SmoI CTYRAG 4 cut(s) 254, 375, 568, 828
Sse9I AATT 2 cut(s) 401, 534
SsiI CCGC 4 cut(s) 493, 501, 770, 1041
SspMI CTAG 1 cut(s) 122
StyD4I CCNGG 2 cut(s) 352, 750
StyI CCWWGG 2 cut(s) 705, 798
TaaI ACNGT 4 cut(s) 159, 322, 392, 787
TaiI ACGT 1 cut(s) 675
TaqI TCGA 2 cut(s) 202, 829
TasI AATT 2 cut(s) 401, 534
TatI WGTACW 2 cut(s) 5, 523
TfiI GAWTC 2 cut(s) 290, 745
Tru1I TTAA 3 cut(s) 255, 596, 984
Tru9I TTAA 3 cut(s) 255, 596, 984
TscAI CASTG 5 cut(s) 30, 281, 343, 391, 905
TseFI GTSAC 4 cut(s) 95, 322, 386, 454
TseI GCWGC 1 cut(s) 285
Tsp45I GTSAC 4 cut(s) 95, 322, 386, 454
TspDTI ATGAA 5 cut(s) 254, 282, 287, 500, 1005
TspGWI ACGGA 2 cut(s) 203, 617
TspRI CASTG 5 cut(s) 30, 281, 343, 391, 905
Vha464I CTTAAG 1 cut(s) 254
VneI GTGCAC 1 cut(s) 601
VpaK11BI GGWCC 3 cut(s) 362, 416, 959
XapI RAATTY 1 cut(s) 401
XcmI CCANNNNNNNNNTGG 1 cut(s) 897
XhoI CTCGAG 1 cut(s) 828
XmnI GAANNNNTTC 1 cut(s) 401
XspI CTAG 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.