pycom17g10580

Protein of unknown function (DUF423)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0002952
Physical Location & Seq
Reverse (-)
8199744 .. 8201154
1411 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 336 bp
ATGGATCCTCAAGTGTGGCACAAAGTAGCCGCCCTCTCTGGAATGGCAGCTCTTGGGTTGGGTACATATGGCGCCCATGGCTTCAAACCCAAAAACCCAACTTACAAAGAGGTTTGGCACACAGCTTCTCTCTACCATTTGGTTCACACGGCAGCTCTTCTTGCTGCCCCTATCACCAAGAACCCCACCATTTTTGGAGGCCTTTTGACTACTGGTATACTCGCATTTTCCGGGACGTGTTATACTGTGGCATTACTCGAAGATAGAAAGTACTCTACGTTGGCCCCTTTCGGTGGCTTTGCTTTCATCGCTGCTTGGGGCAGCTTGCTATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF423

Protein Analysis

112

Amino Acids

11.84

Weight (kDa)

9.17

Isoelectric Point (pI)

17.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF423 PF04241 20 - 99 4.8e-23 Protein of unknown function (DUF423)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 71
AccI GTMKAC 1 cut(s) 217
AciI CCGC 1 cut(s) 30
AclWI GGATC 1 cut(s) 12
AcyI GRCGYC 1 cut(s) 72
AfaI GTAC 2 cut(s) 64, 272
AfiI CCNNNNNNNGG 1 cut(s) 293
AflIII ACRYGT 1 cut(s) 236
AgsI TTSAA 1 cut(s) 85
AjiI CACGTC 1 cut(s) 237
AluBI AGCT 4 cut(s) 50, 125, 155, 324
AluI AGCT 4 cut(s) 50, 125, 155, 324
AlwI GGATC 1 cut(s) 12
AoxI GGCC 2 cut(s) 199, 282
ApeKI GCWGC 5 cut(s) 47, 152, 164, 311, 321
AspLEI GCGC 1 cut(s) 74
AspS9I GGNCC 1 cut(s) 283
AsuC2I CCSGG 1 cut(s) 232
AsuHPI GGTGA 1 cut(s) 166
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 71
BbvI GCAGC 4 cut(s) 59, 151, 164, 298
BceAI ACGGC 1 cut(s) 165
BcnI CCSGG 1 cut(s) 232
BfoI RGCGCY 1 cut(s) 75
BisI GCNGC 6 cut(s) 30, 48, 153, 165, 312, 322
BlsI GCNGC 6 cut(s) 31, 49, 154, 166, 313, 323
BmcAI AGTACT 1 cut(s) 272
Bme1390I CCNGG 1 cut(s) 232
BmgBI CACGTC 1 cut(s) 237
BmgT120I GGNCC 1 cut(s) 283
BmiI GGNNCC 3 cut(s) 6, 73, 285
BmrFI CCNGG 1 cut(s) 232
BpuMI CCSGG 1 cut(s) 232
BsaHI GRCGYC 1 cut(s) 72
BsaJI CCNNGG 1 cut(s) 76
Bsc4I CCNNNNNNNGG 1 cut(s) 293
Bse1I ACTGG 1 cut(s) 217
BseDI CCNNGG 1 cut(s) 76
BseLI CCNNNNNNNGG 1 cut(s) 293
BseNI ACTGG 1 cut(s) 217
BseXI GCAGC 4 cut(s) 59, 151, 164, 298
BshFI GGCC 2 cut(s) 201, 284
BshNI GGYRCC 1 cut(s) 71
BsiSI CCGG 1 cut(s) 231
BslFI GGGAC 1 cut(s) 247
BslI CCNNNNNNNGG 1 cut(s) 293
BsmFI GGGAC 1 cut(s) 247
BsnI GGCC 2 cut(s) 201, 284
Bsp143I GATC 1 cut(s) 4
Bsp19I CCATGG 1 cut(s) 76
BspACI CCGC 1 cut(s) 30
BspANI GGCC 2 cut(s) 201, 284
BspLI GGNNCC 3 cut(s) 6, 73, 285
BspPI GGATC 1 cut(s) 12
BspQI GCTCTTC 1 cut(s) 162
BspT107I GGYRCC 1 cut(s) 71
BsrI ACTGG 1 cut(s) 217
BssECI CCNNGG 1 cut(s) 76
BssMI GATC 1 cut(s) 4
BssNAI GTATAC 1 cut(s) 218
BssNI GRCGYC 1 cut(s) 72
BssT1I CCWWGG 1 cut(s) 76
Bst1107I GTATAC 1 cut(s) 218
Bst4CI ACNGT 1 cut(s) 247
Bst6I CTCTTC 1 cut(s) 162
BstACI GRCGYC 1 cut(s) 72
BstC8I GCNNGC 1 cut(s) 326
BstDSI CCRYGG 1 cut(s) 76
BstH2I RGCGCY 1 cut(s) 75
BstHHI GCGC 1 cut(s) 74
BstKTI GATC 1 cut(s) 7
BstMBI GATC 1 cut(s) 4
BstMWI GCNNNNNNNGC 3 cut(s) 78, 161, 308
BstSCI CCNGG 1 cut(s) 230
BstV1I GCAGC 4 cut(s) 59, 151, 164, 298
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BstZ17I GTATAC 1 cut(s) 218
BsuRI GGCC 2 cut(s) 201, 284
BtgI CCRYGG 1 cut(s) 76
BtgZI GCGATG 1 cut(s) 292
BtrI CACGTC 1 cut(s) 237
Cac8I GCNNGC 1 cut(s) 326
CfoI GCGC 1 cut(s) 74
Cfr13I GGNCC 1 cut(s) 283
Csp6I GTAC 2 cut(s) 63, 271
CspCI CAANNNNNGTGG 2 cut(s) 175, 210
CviAII CATG 1 cut(s) 77
CviJI RGCY 9 cut(s) 29, 50, 81, 125, 155, 201, 284, 297, 324
CviKI_1 RGCY 9 cut(s) 29, 50, 81, 125, 155, 201, 284, 297, 324
CviQI GTAC 2 cut(s) 63, 271
DinI GGCGCC 1 cut(s) 73
DpnI GATC 1 cut(s) 6
DpnII GATC 1 cut(s) 4
Eam1104I CTCTTC 1 cut(s) 162
EarI CTCTTC 1 cut(s) 162
Eco130I CCWWGG 1 cut(s) 76
Eco147I AGGCCT 1 cut(s) 201
EcoT14I CCWWGG 1 cut(s) 76
EgeI GGCGCC 1 cut(s) 73
EheI GGCGCC 1 cut(s) 73
ErhI CCWWGG 1 cut(s) 76
FaeI CATG 1 cut(s) 80
FaiI YATR 5 cut(s) 67, 69, 78, 218, 243
FaqI GGGAC 1 cut(s) 247
FatI CATG 1 cut(s) 76
FauNDI CATATG 1 cut(s) 67
FblI GTMKAC 1 cut(s) 217
Fnu4HI GCNGC 6 cut(s) 30, 48, 153, 165, 312, 322
Fsp4HI GCNGC 6 cut(s) 30, 48, 153, 165, 312, 322
GlaI GCGC 1 cut(s) 73
GluI GCNGC 6 cut(s) 30, 48, 153, 165, 312, 322
HaeII RGCGCY 1 cut(s) 75
HaeIII GGCC 2 cut(s) 201, 284
HapII CCGG 1 cut(s) 231
HhaI GCGC 1 cut(s) 74
Hin1I GRCGYC 1 cut(s) 72
Hin1II CATG 1 cut(s) 80
Hin6I GCGC 1 cut(s) 72
HinP1I GCGC 1 cut(s) 72
HpaII CCGG 1 cut(s) 231
HphI GGTGA 1 cut(s) 166
Hpy166II GTNNAC 2 cut(s) 145, 218
Hpy188III TCNNGA 1 cut(s) 39
Hpy8I GTNNAC 2 cut(s) 145, 218
HpyCH4III ACNGT 1 cut(s) 247
HpyCH4IV ACGT 2 cut(s) 236, 278
HpyF10VI GCNNNNNNNGC 3 cut(s) 78, 161, 308
HpySE526I ACGT 2 cut(s) 236, 278
Hsp92I GRCGYC 1 cut(s) 72
Hsp92II CATG 1 cut(s) 80
HspAI GCGC 1 cut(s) 72
KasI GGCGCC 1 cut(s) 71
Kzo9I GATC 1 cut(s) 4
LguI GCTCTTC 1 cut(s) 162
LpnPI CCDG 3 cut(s) 24, 198, 244
Lsp1109I GCAGC 4 cut(s) 59, 151, 164, 298
MaeII ACGT 2 cut(s) 236, 278
MalI GATC 1 cut(s) 6
MboI GATC 1 cut(s) 4
MboII GAAGA 2 cut(s) 149, 272
MflI RGATCY 1 cut(s) 4
Mly113I GGCGCC 1 cut(s) 72
MnlI CCTC 4 cut(s) 18, 44, 103, 191
MseI TTAA 1 cut(s) 334
MspI CCGG 1 cut(s) 231
MspR9I CCNGG 1 cut(s) 232
MwoI GCNNNNNNNGC 3 cut(s) 78, 161, 308
NarI GGCGCC 1 cut(s) 72
NciI CCSGG 1 cut(s) 232
NcoI CCATGG 1 cut(s) 76
NdeI CATATG 1 cut(s) 67
NdeII GATC 1 cut(s) 4
NlaIII CATG 1 cut(s) 80
NlaIV GGNNCC 3 cut(s) 6, 73, 285
PceI AGGCCT 1 cut(s) 201
PciSI GCTCTTC 1 cut(s) 162
PfoI TCCNGGA 1 cut(s) 230
PkrI GCNGC 6 cut(s) 31, 49, 154, 166, 313, 323
PluTI GGCGCC 1 cut(s) 75
PspN4I GGNNCC 3 cut(s) 6, 73, 285
PspPI GGNCC 1 cut(s) 283
PsuI RGATCY 1 cut(s) 4
RsaI GTAC 2 cut(s) 64, 272
RsaNI GTAC 2 cut(s) 63, 271
SapI GCTCTTC 1 cut(s) 162
SaqAI TTAA 1 cut(s) 334
SatI GCNGC 6 cut(s) 30, 48, 153, 165, 312, 322
Sau3AI GATC 1 cut(s) 4
Sau96I GGNCC 1 cut(s) 283
ScaI AGTACT 1 cut(s) 272
ScrFI CCNGG 1 cut(s) 232
SetI ASST 7 cut(s) 52, 114, 127, 157, 239, 281, 326
SfoI GGCGCC 1 cut(s) 73
SmlI CTYRAG 1 cut(s) 9
SmoI CTYRAG 1 cut(s) 9
SseBI AGGCCT 1 cut(s) 201
SsiI CCGC 1 cut(s) 30
SspDI GGCGCC 1 cut(s) 71
StuI AGGCCT 1 cut(s) 201
StyD4I CCNGG 1 cut(s) 230
StyI CCWWGG 1 cut(s) 76
TaaI ACNGT 1 cut(s) 247
TaiI ACGT 2 cut(s) 239, 281
TaqI TCGA 1 cut(s) 258
TatI WGTACW 1 cut(s) 270
TauI GCSGC 1 cut(s) 32
Tru1I TTAA 1 cut(s) 334
Tru9I TTAA 1 cut(s) 334
TseI GCWGC 5 cut(s) 47, 152, 164, 311, 321
TspDTI ATGAA 1 cut(s) 295
XmiI GTMKAC 1 cut(s) 217
ZrmI AGTACT 1 cut(s) 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.