pycom17g13580

O-methyltransferase that catalyzes the 2 O-methylation steps in the ubiquinone biosynthetic pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0003217
Physical Location & Seq
Forward (+)
11197161 .. 11200533
3373 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1101 bp
ATGGGCCATATTTTGGGCCTTCACCATCCCACTCATAAACCCAACCCAAAGAATGCCTTTGCCGGACTCAATTTGCAGACCAGACCCGGATCTACAAAAACTGAAAAAGAATCCACCGATCCCCTTCCCTTCTTCTTCGTTCAATTTTACTCTCTCAGCAAAATCGCCATGGCTGTGGCTTTGAAGCTCCTGAAGCAACTCCGAGCACCGCACACCACCTCCACAATCCTCCACCGCAATCTCAGCCCACGGCTCTCGGGCATTCACAGTGCAACCAGACTCTTCTCAGACGCTCCTCCTCTAACCGAGCCTCTTCCGGCTCCACCGCCGCTAAACCCTCGTTCTCCTTCCCCGTCGTCTCTGAACCAGTACGAGCTCGCCAAGTTCGCCGCAGTTGCCGAAACCTGGTGGGATTCTGAAGGGCCGTTCAAACCATTGCATATGCTGAATCCTACAAGACTTGCTTTCATTCGCTCCACGCTTTGCCGACATTTCGGGAGGGATCCGTTGTCCCCTAAGCCATTTGAAGGTCTCAAATTTATTGATGTTGGCTGCGGTGGTGGAATTCTTTCTGAGCCTTTAGCTCGAATGGGAGCTACTGTGACGGGAGTTGATGCAGTGGAGAAAAATATCAACATAGCTCGCGTTCACGCTGATTTAGATCCAGTGACTTCGAGAATTGACTATCAATGCACAACAGCTGAAAAGCTAGTTGAGGAACAGAAGAATTTTGATGCTGTGTTTGCTTTGGAGGTAATTGAGCACGTTGCAGACCCTACTGAGTTCTGCAAATCTCTATCAGCCTTAACTGTCCCCAGTGGAGCTACTGTTGTTTCAACTATTAACCGTTCTATGAGAGCATATGCTACTGCCATTGTTGCAGCAGAGTACATCCTCCGCTGGCTTCCTAAAGGTACACATCAATGGTCCAGTTTTCTCACTCCCGAAGAACTAGCCCTGATCCTGGAGCTTGCTTCTATTTCAGTCCAAGAGATGGCTGGGATCGAGTACAATCCTTTGACAGGAAGATGGTCTCTATCTGATGATGTCAGTGTAAATTTTATTGCTTATGGTACCAAAACAGTGACATCCAATTTATAA

Protein Analysis

367

Amino Acids

40.12

Weight (kDa)

7.21

Isoelectric Point (pI)

47.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_23 PF13489 168 - 324 1.4e-19 Methyltransferase domain
Methyltransf_31 PF13847 176 - 310 2.5e-13 Methyltransferase domain
PrmA PF06325 179 - 281 8.7e-07 Ribosomal protein L11 methyltransferase (PrmA)
Methyltransf_11 PF08241 181 - 277 5.4e-18 Methyltransferase domain
Methyltransf_25 PF13649 181 - 274 1.6e-14 Methyltransferase domain
Methyltransf_12 PF08242 181 - 274 3.2e-11 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1099
Acc65I GGTACC 1 cut(s) 1073
AccB1I GGYRCC 1 cut(s) 1073
AccB7I CCANNNNNTGG 2 cut(s) 13, 994
AccII CGCG 1 cut(s) 645
AciI CCGC 7 cut(s) 209, 235, 326, 329, 390, 555, 898
AclWI GGATC 7 cut(s) 97, 113, 497, 510, 656, 955, 1010
AcsI RAATTY 4 cut(s) 536, 564, 727, 1057
AcuI CTGAAG 2 cut(s) 212, 438
AfaI GTAC 5 cut(s) 371, 890, 916, 1010, 1075
AfiI CCNNNNNNNGG 6 cut(s) 13, 405, 527, 964, 994, 1022
AgsI TTSAA 5 cut(s) 143, 184, 430, 527, 837
AjnI CCWGG 2 cut(s) 404, 963
AluBI AGCT 9 cut(s) 187, 376, 584, 596, 641, 701, 709, 824, 970
AluI AGCT 9 cut(s) 187, 376, 584, 596, 641, 701, 709, 824, 970
Alw21I GWGCWC 3 cut(s) 208, 378, 765
Alw26I GTCTC 3 cut(s) 363, 536, 1038
AlwI GGATC 7 cut(s) 97, 113, 497, 510, 656, 955, 1010
Ama87I CYCGRG 1 cut(s) 256
AoxI GGCC 3 cut(s) 4, 16, 422
ApeKI GCWGC 2 cut(s) 552, 881
ApoI RAATTY 4 cut(s) 536, 564, 727, 1057
Asp700I GAANNNNTTC 1 cut(s) 568
Asp718I GGTACC 1 cut(s) 1073
AspS9I GGNCC 4 cut(s) 4, 16, 422, 927
AsuC2I CCSGG 1 cut(s) 87
AsuHPI GGTGA 1 cut(s) 14
AvaI CYCGRG 1 cut(s) 256
AvaII GGWCC 1 cut(s) 927
BamHI GGATCC 1 cut(s) 502
BanI GGYRCC 1 cut(s) 1073
BanII GRGCYC 1 cut(s) 378
Bbv12I GWGCWC 3 cut(s) 208, 378, 765
BbvI GCAGC 2 cut(s) 539, 893
BccI CCATC 3 cut(s) 33, 988, 1023
BceAI ACGGC 2 cut(s) 266, 409
BciT130I CCWGG 2 cut(s) 406, 965
BcnI CCSGG 1 cut(s) 87
BcoDI GTCTC 3 cut(s) 363, 536, 1038
BfaI CTAG 2 cut(s) 710, 953
BisI GCNGC 4 cut(s) 329, 390, 553, 882
BlsI GCNGC 4 cut(s) 330, 391, 554, 883
Bme1390I CCNGG 3 cut(s) 87, 406, 965
Bme18I GGWCC 1 cut(s) 927
BmeT110I CYCGRG 1 cut(s) 256
BmgT120I GGNCC 4 cut(s) 4, 16, 422, 927
BmiI GGNNCC 3 cut(s) 321, 504, 1075
BmrFI CCNGG 3 cut(s) 87, 406, 965
BmrI ACTGGG 1 cut(s) 810
BmsI GCATC 2 cut(s) 604, 724
BmuI ACTGGG 1 cut(s) 810
BpmI CTGGAG 1 cut(s) 986
Bpu10I CCTNAGC 1 cut(s) 516
BpuMI CCSGG 1 cut(s) 87
BsaBI GATNNNNATC 1 cut(s) 660
BsaI GGTCTC 2 cut(s) 536, 1038
BsaJI CCNNGG 2 cut(s) 168, 248
BsaXI ACNNNNNCTCC 6 cut(s) 203, 233, 585, 615, 813, 843
Bsc4I CCNNNNNNNGG 6 cut(s) 13, 405, 527, 964, 994, 1022
Bse1I ACTGG 4 cut(s) 367, 665, 816, 930
Bse3DI GCAATG 1 cut(s) 434
Bse8I GATNNNNATC 1 cut(s) 660
BseBI CCWGG 2 cut(s) 406, 965
BseDI CCNNGG 2 cut(s) 168, 248
BseGI GGATG 3 cut(s) 25, 891, 1088
BseJI GATNNNNATC 1 cut(s) 660
BseLI CCNNNNNNNGG 6 cut(s) 13, 405, 527, 964, 994, 1022
BseMI GCAATG 1 cut(s) 434
BseMII CTCAG 5 cut(s) 169, 256, 300, 564, 771
BseNI ACTGG 4 cut(s) 367, 665, 816, 930
BseRI GAGGAG 2 cut(s) 285, 288
BseXI GCAGC 2 cut(s) 539, 893
BseYI CCCAGC 1 cut(s) 998
Bsh1236I CGCG 1 cut(s) 645
BshFI GGCC 3 cut(s) 6, 18, 424
BshNI GGYRCC 1 cut(s) 1073
BsiHKAI GWGCWC 3 cut(s) 208, 378, 765
BsiHKCI CYCGRG 1 cut(s) 256
BsiSI CCGG 3 cut(s) 63, 87, 317
BslFI GGGAC 2 cut(s) 496, 797
BslI CCNNNNNNNGG 6 cut(s) 13, 405, 527, 964, 994, 1022
BsmAI GTCTC 3 cut(s) 363, 536, 1038
BsmBI CGTCTC 1 cut(s) 363
BsmFI GGGAC 2 cut(s) 496, 797
BsmI GAATGC 2 cut(s) 58, 261
BsnI GGCC 3 cut(s) 6, 18, 424
Bso31I GGTCTC 2 cut(s) 536, 1038
BsoBI CYCGRG 1 cut(s) 256
Bsp1286I GDGCHC 3 cut(s) 208, 378, 765
Bsp143I GATC 6 cut(s) 89, 118, 502, 661, 960, 1002
Bsp19I CCATGG 1 cut(s) 168
BspACI CCGC 7 cut(s) 209, 235, 326, 329, 390, 555, 898
BspANI GGCC 3 cut(s) 6, 18, 424
BspCNI CTCAG 5 cut(s) 168, 255, 299, 565, 772
BspFNI CGCG 1 cut(s) 645
BspLI GGNNCC 3 cut(s) 321, 504, 1075
BspPI GGATC 7 cut(s) 97, 113, 497, 510, 656, 955, 1010
BspT107I GGYRCC 1 cut(s) 1073
BspTNI GGTCTC 2 cut(s) 536, 1038
BsrDI GCAATG 1 cut(s) 434
BsrI ACTGG 4 cut(s) 367, 665, 816, 930
BssECI CCNNGG 2 cut(s) 168, 248
BssMI GATC 6 cut(s) 89, 118, 502, 661, 960, 1002
BssT1I CCWWGG 1 cut(s) 168
Bst2UI CCWGG 2 cut(s) 406, 965
Bst4CI ACNGT 6 cut(s) 269, 601, 811, 829, 848, 1084
Bst6I CTCTTC 2 cut(s) 287, 318
BstC8I GCNNGC 4 cut(s) 378, 643, 902, 972
BstDEI CTNAG 6 cut(s) 155, 242, 286, 516, 573, 780
BstDSI CCRYGG 2 cut(s) 168, 248
BstENI CCTNNNNNAGG 1 cut(s) 1020
BstF5I GGATG 3 cut(s) 25, 891, 1088
BstFNI CGCG 1 cut(s) 645
BstKTI GATC 6 cut(s) 92, 121, 505, 664, 963, 1005
BstMAI GTCTC 3 cut(s) 363, 536, 1038
BstMBI GATC 6 cut(s) 89, 118, 502, 661, 960, 1002
BstMWI GCNNNNNNNGC 6 cut(s) 193, 243, 386, 395, 743, 878
BstNI CCWGG 2 cut(s) 406, 965
BstSCI CCNGG 3 cut(s) 85, 404, 963
BstUI CGCG 1 cut(s) 645
BstV1I GCAGC 2 cut(s) 539, 893
BstX2I RGATCY 3 cut(s) 89, 502, 661
BstXI CCANNNNNNTGG 1 cut(s) 175
BstYI RGATCY 3 cut(s) 89, 502, 661
BsuRI GGCC 3 cut(s) 6, 18, 424
BtgI CCRYGG 2 cut(s) 168, 248
BtsCI GGATG 3 cut(s) 25, 891, 1088
BtsI GCAGTG 1 cut(s) 624
BtsIMutI CAGTG 6 cut(s) 274, 624, 672, 823, 1057, 1089
Cac8I GCNNGC 4 cut(s) 378, 643, 902, 972
Cfr13I GGNCC 4 cut(s) 4, 16, 422, 927
CseI GACGC 1 cut(s) 299
CsiI ACCWGGT 1 cut(s) 404
Csp6I GTAC 5 cut(s) 370, 889, 915, 1009, 1074
CviAII CATG 1 cut(s) 169
CviQI GTAC 5 cut(s) 370, 889, 915, 1009, 1074
DdeI CTNAG 6 cut(s) 155, 242, 286, 516, 573, 780
DpnI GATC 6 cut(s) 91, 120, 504, 663, 962, 1004
DpnII GATC 6 cut(s) 89, 118, 502, 661, 960, 1002
Eam1104I CTCTTC 2 cut(s) 287, 318
EarI CTCTTC 2 cut(s) 287, 318
Ecl136II GAGCTC 1 cut(s) 376
Eco130I CCWWGG 1 cut(s) 168
Eco24I GRGCYC 1 cut(s) 378
Eco31I GGTCTC 2 cut(s) 536, 1038
Eco47I GGWCC 1 cut(s) 927
Eco53kI GAGCTC 1 cut(s) 376
Eco57I CTGAAG 2 cut(s) 212, 438
Eco88I CYCGRG 1 cut(s) 256
EcoICRI GAGCTC 1 cut(s) 376
EcoNI CCTNNNNNAGG 1 cut(s) 1020
EcoRI GAATTC 1 cut(s) 564
EcoRII CCWGG 2 cut(s) 404, 963
EcoT14I CCWWGG 1 cut(s) 168
EcoT38I GRGCYC 1 cut(s) 378
ErhI CCWWGG 1 cut(s) 168
Esp3I CGTCTC 1 cut(s) 363
FaeI CATG 1 cut(s) 172
FalI AAGNNNNNCTT 4 cut(s) 41, 73, 448, 480
FaqI GGGAC 2 cut(s) 496, 797
FatI CATG 1 cut(s) 168
FauNDI CATATG 2 cut(s) 441, 862
Fnu4HI GCNGC 4 cut(s) 329, 390, 553, 882
FokI GGATG 3 cut(s) 12, 878, 1075
FriOI GRGCYC 1 cut(s) 378
Fsp4HI GCNGC 4 cut(s) 329, 390, 553, 882
FspBI CTAG 2 cut(s) 710, 953
GluI GCNGC 4 cut(s) 329, 390, 553, 882
GsaI CCCAGC 1 cut(s) 1002
GsuI CTGGAG 1 cut(s) 986
HaeIII GGCC 3 cut(s) 6, 18, 424
HapII CCGG 3 cut(s) 63, 87, 317
HgaI GACGC 1 cut(s) 299
Hin1II CATG 1 cut(s) 172
HinfI GANTC 5 cut(s) 66, 110, 279, 413, 448
HpaII CCGG 3 cut(s) 63, 87, 317
HphI GGTGA 1 cut(s) 14
Hpy166II GTNNAC 2 cut(s) 649, 917
Hpy188I TCNGA 6 cut(s) 203, 289, 363, 418, 574, 1042
Hpy188III TCNNGA 4 cut(s) 190, 496, 675, 944
Hpy8I GTNNAC 2 cut(s) 649, 917
Hpy99I CGWCG 1 cut(s) 358
HpyAV CCTTC 6 cut(s) 29, 134, 139, 357, 413, 521
HpyCH4III ACNGT 6 cut(s) 269, 601, 811, 829, 848, 1084
HpyCH4IV ACGT 1 cut(s) 765
HpyCH4V TGCA 8 cut(s) 76, 272, 439, 617, 693, 770, 789, 881
HpyF10VI GCNNNNNNNGC 6 cut(s) 193, 243, 386, 395, 743, 878
HpyF3I CTNAG 6 cut(s) 155, 242, 286, 516, 573, 780
HpySE526I ACGT 1 cut(s) 765
Hsp92II CATG 1 cut(s) 172
KpnI GGTACC 1 cut(s) 1077
Kzo9I GATC 6 cut(s) 89, 118, 502, 661, 960, 1002
LmnI GCTCC 7 cut(s) 192, 298, 325, 479, 593, 821, 967
Lsp1109I GCAGC 2 cut(s) 539, 893
LweI GCATC 2 cut(s) 604, 724
MabI ACCWGGT 1 cut(s) 404
MaeI CTAG 2 cut(s) 710, 953
MaeII ACGT 1 cut(s) 765
MaeIII GTNAC 3 cut(s) 601, 667, 1084
MalI GATC 6 cut(s) 91, 120, 504, 663, 962, 1004
MboI GATC 6 cut(s) 89, 118, 502, 661, 960, 1002
MboII GAAGA 7 cut(s) 124, 127, 274, 305, 736, 959, 1038
MflI RGATCY 3 cut(s) 89, 502, 661
MhlI GDGCHC 3 cut(s) 208, 378, 765
MluCI AATT 9 cut(s) 70, 143, 536, 564, 678, 727, 756, 1057, 1093
MlyI GAGTC 2 cut(s) 60, 273
MroXI GAANNNNTTC 1 cut(s) 568
MseI TTAA 2 cut(s) 806, 843
MslI CAYNNNNRTG 2 cut(s) 173, 922
MspA1I CMGCKG 2 cut(s) 701, 900
MspI CCGG 3 cut(s) 63, 87, 317
MspR9I CCNGG 3 cut(s) 87, 406, 965
Mva1269I GAATGC 2 cut(s) 58, 261
MvaI CCWGG 2 cut(s) 406, 965
MvnI CGCG 1 cut(s) 645
MwoI GCNNNNNNNGC 6 cut(s) 193, 243, 386, 395, 743, 878
NciI CCSGG 1 cut(s) 87
NcoI CCATGG 1 cut(s) 168
NdeI CATATG 2 cut(s) 441, 862
NdeII GATC 6 cut(s) 89, 118, 502, 661, 960, 1002
NlaIII CATG 1 cut(s) 172
NlaIV GGNNCC 3 cut(s) 321, 504, 1075
NmuCI GTSAC 3 cut(s) 601, 667, 1084
PctI GAATGC 2 cut(s) 58, 261
PdmI GAANNNNTTC 1 cut(s) 568
PfeI GAWTC 3 cut(s) 110, 413, 448
PflMI CCANNNNNTGG 2 cut(s) 13, 994
PfoI TCCNGGA 1 cut(s) 963
PkrI GCNGC 4 cut(s) 330, 391, 554, 883
PleI GAGTC 2 cut(s) 60, 273
PpsI GAGTC 2 cut(s) 60, 273
PsiI TTATAA 1 cut(s) 1099
Psp124BI GAGCTC 1 cut(s) 378
Psp6I CCWGG 2 cut(s) 404, 963
PspFI CCCAGC 1 cut(s) 998
PspGI CCWGG 2 cut(s) 404, 963
PspN4I GGNNCC 3 cut(s) 321, 504, 1075
PspPI GGNCC 4 cut(s) 4, 16, 422, 927
PsuI RGATCY 3 cut(s) 89, 502, 661
PvuII CAGCTG 1 cut(s) 701
RsaI GTAC 5 cut(s) 371, 890, 916, 1010, 1075
RsaNI GTAC 5 cut(s) 370, 889, 915, 1009, 1074
RseI CAYNNNNRTG 2 cut(s) 173, 922
SacI GAGCTC 1 cut(s) 378
SaqAI TTAA 2 cut(s) 806, 843
SatI GCNGC 4 cut(s) 329, 390, 553, 882
Sau3AI GATC 6 cut(s) 89, 118, 502, 661, 960, 1002
Sau96I GGNCC 4 cut(s) 4, 16, 422, 927
SchI GAGTC 2 cut(s) 60, 273
ScrFI CCNGG 3 cut(s) 87, 406, 965
SduI GDGCHC 3 cut(s) 208, 378, 765
SexAI ACCWGGT 1 cut(s) 404
SfaNI GCATC 2 cut(s) 604, 724
SinI GGWCC 1 cut(s) 927
SmiMI CAYNNNNRTG 2 cut(s) 173, 922
Sse9I AATT 9 cut(s) 70, 143, 536, 564, 678, 727, 756, 1057, 1093
SsiI CCGC 7 cut(s) 209, 235, 326, 329, 390, 555, 898
SspMI CTAG 2 cut(s) 710, 953
SstI GAGCTC 1 cut(s) 378
StyD4I CCNGG 3 cut(s) 85, 404, 963
StyI CCWWGG 1 cut(s) 168
TaaI ACNGT 6 cut(s) 269, 601, 811, 829, 848, 1084
TaiI ACGT 1 cut(s) 768
TaqI TCGA 3 cut(s) 586, 674, 1005
TasI AATT 9 cut(s) 70, 143, 536, 564, 678, 727, 756, 1057, 1093
TatI WGTACW 2 cut(s) 888, 1008
TauI GCSGC 2 cut(s) 331, 392
TfiI GAWTC 3 cut(s) 110, 413, 448
Tru1I TTAA 2 cut(s) 806, 843
Tru9I TTAA 2 cut(s) 806, 843
TscAI CASTG 6 cut(s) 274, 624, 672, 823, 1057, 1089
TseFI GTSAC 3 cut(s) 601, 667, 1084
TseI GCWGC 2 cut(s) 552, 881
Tsp45I GTSAC 3 cut(s) 601, 667, 1084
TspDTI ATGAA 1 cut(s) 457
TspGWI ACGGA 1 cut(s) 495
TspRI CASTG 6 cut(s) 274, 624, 672, 823, 1057, 1089
Van91I CCANNNNNTGG 2 cut(s) 13, 994
VpaK11BI GGWCC 1 cut(s) 927
XagI CCTNNNNNAGG 1 cut(s) 1020
XapI RAATTY 4 cut(s) 536, 564, 727, 1057
XcmI CCANNNNNNNNNTGG 1 cut(s) 995
XmnI GAANNNNTTC 1 cut(s) 568
XspI CTAG 2 cut(s) 710, 953
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.