pycom17g19740

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0003380
Physical Location & Seq
Reverse (-)
17685263 .. 17687510
2248 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 996 bp
ATGTCTAGGCCACTAGAGCTTGATTACATAGGCCTAACAGAGACTTCATCTTCTCCAATGGAAAGAAGCTCTGAGAAGATTTCATCTTCCACTTCCTCTACCCTCTCTTCCACAACAGCTGAGGAAGAGAGGACCTCCTCCTCTGCCATCAACCTCAGGGAGACTGAGCTCAGGCTCGGCCTTCCTGGGTCCCACTCCCCTGACAGAAAAAAACTGTCAGGGGTCTCAATTTTTGGGAAAGATTTGGAGGACAACAACAAGCCAAATGGGTTTTCTCCAAATCCTCCAAAGAACCCTGTGTCAGGGGCAAAAAGGGGATTCTCCGACGCCATTGATGGGTGTGAGAAATGGGTTTTCTCCATGGGTAATGGATCTGAGGTTGATGTGGGTAACGCAGCAGTCTTGGGTTCTCCATTAGCCAAGAAAGAGGTTTCTTCTGTTCCTCTGCCACCAAAGCCATCAGCTCAGTTGGAGAAGAAGAAGCCTCAGGCCTCTGAGCATGCTGCTGCTCCTCCTGCTGCCAAGGCACAGGTGGTAGGATGGCCCCCAATTCGATCGTTTCGGAAGAACTCCATGGCCTCTAATTTGGCGAAGAACAACGACGATGCTGAGGGCAAACAAGGGTCCGGGTGCCTGTATGTGAAGGTTGGCATGGATGGTGCTCCGTACCTACGAAAGGTTGACCTTAAAACGTACAACAACTACACTGAACTTTCCATGGCTCTGGAGAAGATGTTTAGCTGCTTCACCATTGGGCAGTGCAGTTGTAATGGACTTCCGGAGCGAGATGGTCTGAGTGCGAGTCGTCTGATGGATCTTCTCAACGGTTCTGAATTTGTTCTGACTTATGAGGACAGGGATGATGACTGGATGCTTGTTGGTGATGTTCCTTGGCAGATGTTCACCGAGACCTGTAGGAGGCTGAGGATCATGAAAGGTTCCGAAGCAATTGGGCTAGCTCCAAGGGCCGTGGAGAAATGCAAGAACCGGAACTAA

Protein Analysis

332

Amino Acids

35.91

Weight (kDa)

7.51

Isoelectric Point (pI)

46.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 54 - 322 1.1e-88 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 630
AccIII TCCGGA 1 cut(s) 778
AclWI GGATC 3 cut(s) 379, 822, 935
AcsI RAATTY 1 cut(s) 833
AcyI GRCGYC 1 cut(s) 327
AfaI GTAC 2 cut(s) 668, 695
AfiI CCNNNNNNNGG 4 cut(s) 302, 336, 676, 918
AjnI CCWGG 1 cut(s) 184
AjuI GAANNNNNNNTTGG 2 cut(s) 256, 288
AluBI AGCT 7 cut(s) 19, 69, 119, 169, 464, 741, 959
AluI AGCT 7 cut(s) 19, 69, 119, 169, 464, 741, 959
Alw21I GWGCWC 2 cut(s) 171, 664
Alw26I GTCTC 4 cut(s) 35, 155, 229, 902
AlwI GGATC 3 cut(s) 379, 822, 935
Aor13HI TCCGGA 1 cut(s) 778
AoxI GGCC 7 cut(s) 8, 31, 178, 489, 542, 576, 966
ApeKI GCWGC 5 cut(s) 395, 503, 506, 518, 741
ApoI RAATTY 1 cut(s) 833
Asp700I GAANNNNTTC 1 cut(s) 837
AspS9I GGNCC 5 cut(s) 132, 189, 543, 624, 966
AsuC2I CCSGG 1 cut(s) 628
AsuHPI GGTGA 3 cut(s) 739, 893, 895
AsuNHI GCTAGC 1 cut(s) 955
AvaII GGWCC 3 cut(s) 132, 189, 624
AxyI CCTNAGG 2 cut(s) 155, 486
BanI GGYRCC 1 cut(s) 630
BanII GRGCYC 1 cut(s) 171
Bbv12I GWGCWC 2 cut(s) 171, 664
BbvCI CCTCAGC 3 cut(s) 120, 609, 923
BbvI GCAGC 5 cut(s) 407, 490, 493, 505, 728
BccI CCATC 7 cut(s) 155, 329, 466, 534, 650, 782, 805
BceAI ACGGC 1 cut(s) 953
BciT130I CCWGG 1 cut(s) 186
BcnI CCSGG 1 cut(s) 628
BcoDI GTCTC 4 cut(s) 35, 155, 229, 902
BfaI CTAG 3 cut(s) 6, 14, 956
BfmI CTRYAG 1 cut(s) 913
BisI GCNGC 5 cut(s) 396, 504, 507, 519, 742
BlsI GCNGC 5 cut(s) 397, 505, 508, 520, 743
Bme1390I CCNGG 2 cut(s) 186, 628
Bme18I GGWCC 3 cut(s) 132, 189, 624
BmgT120I GGNCC 5 cut(s) 132, 189, 543, 624, 966
BmiI GGNNCC 6 cut(s) 190, 191, 545, 625, 632, 940
BmrFI CCNGG 2 cut(s) 186, 628
BmsI GCATC 2 cut(s) 595, 861
BmtI GCTAGC 1 cut(s) 959
BplI GAGNNNNNCTC 2 cut(s) 119, 151
BpmI CTGGAG 1 cut(s) 746
Bpu10I CCTNAGC 4 cut(s) 120, 170, 609, 923
BpuMI CCSGG 1 cut(s) 628
BsaHI GRCGYC 1 cut(s) 327
BsaI GGTCTC 2 cut(s) 229, 902
BsaJI CCNNGG 8 cut(s) 185, 360, 522, 573, 717, 890, 962, 969
BsaWI WCCGGW 2 cut(s) 778, 987
BsaXI ACNNNNNCTCC 2 cut(s) 719, 749
Bsc4I CCNNNNNNNGG 4 cut(s) 302, 336, 676, 918
Bse1I ACTGG 1 cut(s) 872
Bse21I CCTNAGG 2 cut(s) 155, 486
BseAI TCCGGA 1 cut(s) 778
BseBI CCWGG 1 cut(s) 186
BseDI CCNNGG 8 cut(s) 185, 360, 522, 573, 717, 890, 962, 969
BseGI GGATG 4 cut(s) 545, 661, 865, 876
BseLI CCNNNNNNNGG 4 cut(s) 302, 336, 676, 918
BseNI ACTGG 1 cut(s) 872
BseRI GAGGAG 3 cut(s) 127, 130, 501
BseXI GCAGC 5 cut(s) 407, 490, 493, 505, 728
BsgI GTGCAG 1 cut(s) 781
Bsh1285I CGRYCG 1 cut(s) 557
BshFI GGCC 7 cut(s) 10, 33, 180, 491, 544, 578, 968
BshNI GGYRCC 1 cut(s) 630
BsiEI CGRYCG 1 cut(s) 557
BsiHKAI GWGCWC 2 cut(s) 171, 664
BsiSI CCGG 3 cut(s) 627, 779, 988
BslFI GGGAC 1 cut(s) 175
BslI CCNNNNNNNGG 4 cut(s) 302, 336, 676, 918
BsmAI GTCTC 4 cut(s) 35, 155, 229, 902
BsmFI GGGAC 1 cut(s) 175
BsnI GGCC 7 cut(s) 10, 33, 180, 491, 544, 578, 968
Bso31I GGTCTC 2 cut(s) 229, 902
Bsp1286I GDGCHC 2 cut(s) 171, 664
Bsp13I TCCGGA 1 cut(s) 778
Bsp143I GATC 4 cut(s) 371, 554, 814, 927
Bsp19I CCATGG 3 cut(s) 360, 573, 717
BspANI GGCC 7 cut(s) 10, 33, 180, 491, 544, 578, 968
BspEI TCCGGA 1 cut(s) 778
BspHI TCATGA 1 cut(s) 930
BspLI GGNNCC 6 cut(s) 190, 191, 545, 625, 632, 940
BspOI GCTAGC 1 cut(s) 959
BspPI GGATC 3 cut(s) 379, 822, 935
BspT107I GGYRCC 1 cut(s) 630
BspTNI GGTCTC 2 cut(s) 229, 902
BsrI ACTGG 1 cut(s) 872
BssECI CCNNGG 8 cut(s) 185, 360, 522, 573, 717, 890, 962, 969
BssMI GATC 4 cut(s) 371, 554, 814, 927
BssNI GRCGYC 1 cut(s) 327
BssT1I CCWWGG 6 cut(s) 360, 522, 573, 717, 890, 962
Bst2UI CCWGG 1 cut(s) 186
Bst4CI ACNGT 2 cut(s) 216, 827
Bst6I CTCTTC 2 cut(s) 112, 120
BstACI GRCGYC 1 cut(s) 327
BstC8I GCNNGC 2 cut(s) 501, 957
BstDSI CCRYGG 4 cut(s) 360, 573, 717, 969
BstENI CCTNNNNNAGG 3 cut(s) 300, 674, 916
BstF5I GGATG 4 cut(s) 545, 661, 865, 876
BstKTI GATC 4 cut(s) 374, 557, 817, 930
BstMAI GTCTC 4 cut(s) 35, 155, 229, 902
BstMBI GATC 4 cut(s) 371, 554, 814, 927
BstMCI CGRYCG 1 cut(s) 557
BstMWI GCNNNNNNNGC 5 cut(s) 16, 454, 515, 524, 965
BstNI CCWGG 1 cut(s) 186
BstNSI RCATGY 1 cut(s) 503
BstSCI CCNGG 2 cut(s) 184, 626
BstSFI CTRYAG 1 cut(s) 913
BstV1I GCAGC 5 cut(s) 407, 490, 493, 505, 728
BstX2I RGATCY 2 cut(s) 371, 814
BstXI CCANNNNNNTGG 1 cut(s) 724
BstYI RGATCY 2 cut(s) 371, 814
Bsu36I CCTNAGG 2 cut(s) 155, 486
BsuRI GGCC 7 cut(s) 10, 33, 180, 491, 544, 578, 968
BtgI CCRYGG 4 cut(s) 360, 573, 717, 969
BtsCI GGATG 4 cut(s) 545, 661, 865, 876
BtsI GCAGTG 1 cut(s) 764
BtsIMutI CAGTG 2 cut(s) 705, 764
Cac8I GCNNGC 2 cut(s) 501, 957
CciI TCATGA 1 cut(s) 930
Cfr13I GGNCC 5 cut(s) 132, 189, 543, 624, 966
CseI GACGC 1 cut(s) 335
Csp6I GTAC 2 cut(s) 667, 694
CspCI CAANNNNNGTGG 2 cut(s) 951, 986
CviAII CATG 6 cut(s) 361, 500, 574, 652, 718, 931
CviQI GTAC 2 cut(s) 667, 694
DpnI GATC 4 cut(s) 373, 556, 816, 929
DpnII GATC 4 cut(s) 371, 554, 814, 927
Eam1104I CTCTTC 2 cut(s) 112, 120
EarI CTCTTC 2 cut(s) 112, 120
Ecl136II GAGCTC 1 cut(s) 169
Eco130I CCWWGG 6 cut(s) 360, 522, 573, 717, 890, 962
Eco147I AGGCCT 2 cut(s) 33, 491
Eco24I GRGCYC 1 cut(s) 171
Eco31I GGTCTC 2 cut(s) 229, 902
Eco47I GGWCC 3 cut(s) 132, 189, 624
Eco53kI GAGCTC 1 cut(s) 169
Eco81I CCTNAGG 2 cut(s) 155, 486
EcoICRI GAGCTC 1 cut(s) 169
EcoNI CCTNNNNNAGG 3 cut(s) 300, 674, 916
EcoO109I RGGNCCY 2 cut(s) 132, 189
EcoRII CCWGG 1 cut(s) 184
EcoT14I CCWWGG 6 cut(s) 360, 522, 573, 717, 890, 962
EcoT38I GRGCYC 1 cut(s) 171
ErhI CCWWGG 6 cut(s) 360, 522, 573, 717, 890, 962
FaeI CATG 6 cut(s) 364, 503, 577, 655, 721, 934
FaiI YATR 9 cut(s) 29, 362, 501, 575, 639, 653, 719, 849, 932
FaqI GGGAC 1 cut(s) 175
FatI CATG 6 cut(s) 360, 499, 573, 651, 717, 930
Fnu4HI GCNGC 5 cut(s) 396, 504, 507, 519, 742
FokI GGATG 4 cut(s) 552, 668, 872, 883
FriOI GRGCYC 1 cut(s) 171
Fsp4HI GCNGC 5 cut(s) 396, 504, 507, 519, 742
FspBI CTAG 3 cut(s) 6, 14, 956
GluI GCNGC 5 cut(s) 396, 504, 507, 519, 742
GsuI CTGGAG 1 cut(s) 746
HaeIII GGCC 7 cut(s) 10, 33, 180, 491, 544, 578, 968
HapII CCGG 3 cut(s) 627, 779, 988
HgaI GACGC 1 cut(s) 335
Hin1I GRCGYC 1 cut(s) 327
Hin1II CATG 6 cut(s) 364, 503, 577, 655, 721, 934
HincII GTYRAC 1 cut(s) 682
HindII GTYRAC 1 cut(s) 682
HinfI GANTC 2 cut(s) 318, 802
HpaII CCGG 3 cut(s) 627, 779, 988
HphI GGTGA 3 cut(s) 739, 893, 895
Hpy166II GTNNAC 2 cut(s) 682, 903
Hpy188III TCNNGA 3 cut(s) 725, 779, 931
Hpy8I GTNNAC 2 cut(s) 682, 903
Hpy99I CGWCG 2 cut(s) 329, 605
HpyAV CCTTC 2 cut(s) 191, 637
HpyCH4III ACNGT 2 cut(s) 216, 827
HpyCH4IV ACGT 1 cut(s) 692
HpyCH4V TGCA 2 cut(s) 762, 981
HpyF10VI GCNNNNNNNGC 5 cut(s) 16, 454, 515, 524, 965
HpySE526I ACGT 1 cut(s) 692
Hsp92I GRCGYC 1 cut(s) 327
Hsp92II CATG 6 cut(s) 364, 503, 577, 655, 721, 934
KflI GGGWCCC 1 cut(s) 189
Kpn2I TCCGGA 1 cut(s) 778
Kzo9I GATC 4 cut(s) 371, 554, 814, 927
LmnI GCTCC 4 cut(s) 514, 667, 781, 964
Lsp1109I GCAGC 5 cut(s) 407, 490, 493, 505, 728
LweI GCATC 2 cut(s) 595, 861
MaeI CTAG 3 cut(s) 6, 14, 956
MaeII ACGT 1 cut(s) 692
MaeIII GTNAC 1 cut(s) 389
MalI GATC 4 cut(s) 373, 556, 816, 929
MboI GATC 4 cut(s) 371, 554, 814, 927
MfeI CAATTG 1 cut(s) 948
MflI RGATCY 2 cut(s) 371, 814
MhlI GDGCHC 2 cut(s) 171, 664
MluCI AATT 5 cut(s) 228, 549, 583, 833, 948
MlyI GAGTC 1 cut(s) 811
MmeI TCCRAC 2 cut(s) 348, 450
MroI TCCGGA 1 cut(s) 778
MroXI GAANNNNTTC 1 cut(s) 837
MseI TTAA 1 cut(s) 687
MspA1I CMGCKG 1 cut(s) 119
MspI CCGG 3 cut(s) 627, 779, 988
MspR9I CCNGG 2 cut(s) 186, 628
MunI CAATTG 1 cut(s) 948
MvaI CCWGG 1 cut(s) 186
MwoI GCNNNNNNNGC 5 cut(s) 16, 454, 515, 524, 965
NciI CCSGG 1 cut(s) 628
NcoI CCATGG 3 cut(s) 360, 573, 717
NdeII GATC 4 cut(s) 371, 554, 814, 927
NheI GCTAGC 1 cut(s) 955
NlaIII CATG 6 cut(s) 364, 503, 577, 655, 721, 934
NlaIV GGNNCC 6 cut(s) 190, 191, 545, 625, 632, 940
NmeAIII GCCGAG 1 cut(s) 156
NspI RCATGY 1 cut(s) 503
PaeI GCATGC 1 cut(s) 503
PagI TCATGA 1 cut(s) 930
PceI AGGCCT 2 cut(s) 33, 491
PdmI GAANNNNTTC 1 cut(s) 837
PfeI GAWTC 1 cut(s) 318
PkrI GCNGC 5 cut(s) 397, 505, 508, 520, 743
Ple19I CGATCG 1 cut(s) 557
PleI GAGTC 1 cut(s) 810
PpsI GAGTC 1 cut(s) 810
PpuMI RGGWCCY 2 cut(s) 132, 189
Psp124BI GAGCTC 1 cut(s) 171
Psp5II RGGWCCY 2 cut(s) 132, 189
Psp6I CCWGG 1 cut(s) 184
PspGI CCWGG 1 cut(s) 184
PspN4I GGNNCC 6 cut(s) 190, 191, 545, 625, 632, 940
PspPI GGNCC 5 cut(s) 132, 189, 543, 624, 966
PspPPI RGGWCCY 2 cut(s) 132, 189
PsuI RGATCY 2 cut(s) 371, 814
PvuI CGATCG 1 cut(s) 557
PvuII CAGCTG 1 cut(s) 119
RsaI GTAC 2 cut(s) 668, 695
RsaNI GTAC 2 cut(s) 667, 694
SacI GAGCTC 1 cut(s) 171
SaqAI TTAA 1 cut(s) 687
SatI GCNGC 5 cut(s) 396, 504, 507, 519, 742
Sau3AI GATC 4 cut(s) 371, 554, 814, 927
Sau96I GGNCC 5 cut(s) 132, 189, 543, 624, 966
SchI GAGTC 1 cut(s) 811
ScrFI CCNGG 2 cut(s) 186, 628
SduI GDGCHC 2 cut(s) 171, 664
SfaNI GCATC 2 cut(s) 595, 861
SfcI CTRYAG 1 cut(s) 913
SinI GGWCC 3 cut(s) 132, 189, 624
SphI GCATGC 1 cut(s) 503
Sse9I AATT 5 cut(s) 228, 549, 583, 833, 948
SseBI AGGCCT 2 cut(s) 33, 491
SspMI CTAG 3 cut(s) 6, 14, 956
SstI GAGCTC 1 cut(s) 171
StuI AGGCCT 2 cut(s) 33, 491
StyD4I CCNGG 2 cut(s) 184, 626
StyI CCWWGG 6 cut(s) 360, 522, 573, 717, 890, 962
TaaI ACNGT 2 cut(s) 216, 827
TaiI ACGT 1 cut(s) 695
TaqI TCGA 1 cut(s) 553
TasI AATT 5 cut(s) 228, 549, 583, 833, 948
TfiI GAWTC 1 cut(s) 318
Tru1I TTAA 1 cut(s) 687
Tru9I TTAA 1 cut(s) 687
TscAI CASTG 2 cut(s) 712, 764
TseI GCWGC 5 cut(s) 395, 503, 506, 518, 741
TspDTI ATGAA 3 cut(s) 36, 72, 947
TspGWI ACGGA 1 cut(s) 654
TspRI CASTG 2 cut(s) 712, 764
VpaK11BI GGWCC 3 cut(s) 132, 189, 624
XagI CCTNNNNNAGG 3 cut(s) 300, 674, 916
XapI RAATTY 1 cut(s) 833
XceI RCATGY 1 cut(s) 503
XcmI CCANNNNNNNNNTGG 1 cut(s) 529
XmnI GAANNNNTTC 1 cut(s) 837
XspI CTAG 3 cut(s) 6, 14, 956
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.