pycom17g23480

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0002484
Physical Location & Seq
Reverse (-)
21829264 .. 21830322
1059 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 546 bp
ATGACTCTAGAAGATTACCTTCTCCTCATTCAATCCAACTCCCACCTCCATCTCACCGTCGCTCATCTCAATCAGATCATTTCCATGCACGGATACAAGAAGATCTACAAAGTTCCCAAGGCACGTTTGAGCGACGCCGTGAGCTCGCTGCCGCTGGTCGATCCGGCTCGCTCGACGCTCAAAGACTACATCTCTCCATTTGTGATCACCACCCTGGAGGACGTCGTCGCCGACCTCGCCGACCTCAACTGGAAGGAGTGCTGCGTCACATCCGTCGAAACCCTAAGCTCATGGAAACACACAACCTCCACCCCTGCTTCTCCTGTGAGCCCCAGCCACGACGTCGTCCAGTACTTGCAACTTCAACAATCACCGCCGTTGGCCCTTGATTGCAGACCCTACGGCGTCGTTTCTGCTCCCAACTCTGCTTCCGGGACTGCTTGTCCAGAGAAGAAACTGGTGCCGAAGAGGAAGAGGAAGCGATCGCCGGTAGCCGCCGGTTTCGCTCGTGCTGCTCTGGACTCTGTTTCGTACGGATCGTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

182

Amino Acids

19.72

Weight (kDa)

8.79

Isoelectric Point (pI)

62.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7787 PF25042 1 - 54 4.7e-23 Domain of unknown function (DUF7787)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 225, 345
AccB1I GGYRCC 1 cut(s) 460
AciI CCGC 3 cut(s) 152, 374, 495
AclWI GGATC 2 cut(s) 155, 544
AcyI GRCGYC 4 cut(s) 135, 222, 342, 405
AfaI GTAC 2 cut(s) 353, 533
AgsI TTSAA 2 cut(s) 32, 365
AhdI GACNNNNNGTC 1 cut(s) 441
AjnI CCWGG 1 cut(s) 213
AluBI AGCT 2 cut(s) 144, 288
AluI AGCT 2 cut(s) 144, 288
Alw21I GWGCWC 1 cut(s) 146
AlwI GGATC 2 cut(s) 155, 544
AoxI GGCC 1 cut(s) 381
ApeKI GCWGC 3 cut(s) 148, 261, 512
AsiSI GCGATCGC 1 cut(s) 485
AspS9I GGNCC 1 cut(s) 382
AsuC2I CCSGG 1 cut(s) 433
AsuHPI GGTGA 3 cut(s) 46, 199, 363
BanI GGYRCC 1 cut(s) 460
BanII GRGCYC 2 cut(s) 146, 332
BauI CACGAG 1 cut(s) 507
Bbv12I GWGCWC 1 cut(s) 146
BbvI GCAGC 3 cut(s) 135, 248, 499
BccI CCATC 1 cut(s) 57
BceAI ACGGC 3 cut(s) 122, 361, 418
BciT130I CCWGG 1 cut(s) 215
BciVI GTATCC 1 cut(s) 86
BclI TGATCA 1 cut(s) 204
BcnI CCSGG 1 cut(s) 433
BfaI CTAG 1 cut(s) 8
BfuI GTATCC 1 cut(s) 86
BglII AGATCT 1 cut(s) 102
BisI GCNGC 5 cut(s) 149, 152, 262, 495, 513
BlsI GCNGC 5 cut(s) 150, 153, 263, 496, 514
BmcAI AGTACT 1 cut(s) 353
Bme1390I CCNGG 2 cut(s) 215, 433
BmeRI GACNNNNNGTC 1 cut(s) 441
BmgT120I GGNCC 1 cut(s) 382
BmiI GGNNCC 1 cut(s) 462
BmrFI CCNGG 2 cut(s) 215, 433
BpmI CTGGAG 1 cut(s) 236
Bpu10I CCTNAGC 1 cut(s) 284
BpuMI CCSGG 1 cut(s) 433
BsaHI GRCGYC 4 cut(s) 135, 222, 342, 405
BsaJI CCNNGG 2 cut(s) 117, 213
BsaXI ACNNNNNCTCC 2 cut(s) 290, 320
Bse118I RCCGGY 2 cut(s) 487, 497
Bse1I ACTGG 3 cut(s) 254, 349, 462
BseBI CCWGG 1 cut(s) 215
BseDI CCNNGG 2 cut(s) 117, 213
BseGI GGATG 1 cut(s) 269
BseNI ACTGG 3 cut(s) 254, 349, 462
BseRI GAGGAG 1 cut(s) 14
BseXI GCAGC 3 cut(s) 135, 248, 499
BseYI CCCAGC 1 cut(s) 332
Bsh1285I CGRYCG 1 cut(s) 485
BshFI GGCC 1 cut(s) 383
BshNI GGYRCC 1 cut(s) 460
BsiEI CGRYCG 1 cut(s) 485
BsiHKAI GWGCWC 1 cut(s) 146
BsiSI CCGG 4 cut(s) 164, 432, 488, 498
BsiWI CGTACG 1 cut(s) 531
BslFI GGGAC 1 cut(s) 448
BsmFI GGGAC 1 cut(s) 448
BsnI GGCC 1 cut(s) 383
Bsp1286I GDGCHC 2 cut(s) 146, 332
Bsp143I GATC 6 cut(s) 75, 102, 160, 204, 482, 536
BspACI CCGC 3 cut(s) 152, 374, 495
BspANI GGCC 1 cut(s) 383
BspLI GGNNCC 1 cut(s) 462
BspPI GGATC 2 cut(s) 155, 544
BspT107I GGYRCC 1 cut(s) 460
BsrFI RCCGGY 2 cut(s) 487, 497
BsrI ACTGG 3 cut(s) 254, 349, 462
BssAI RCCGGY 2 cut(s) 487, 497
BssECI CCNNGG 2 cut(s) 117, 213
BssMI GATC 6 cut(s) 75, 102, 160, 204, 482, 536
BssNI GRCGYC 4 cut(s) 135, 222, 342, 405
BssSI CACGAG 1 cut(s) 507
BssT1I CCWWGG 1 cut(s) 117
Bst2BI CACGAG 1 cut(s) 507
Bst2UI CCWGG 1 cut(s) 215
Bst4CI ACNGT 1 cut(s) 58
Bst6I CTCTTC 2 cut(s) 461, 467
BstACI GRCGYC 4 cut(s) 135, 222, 342, 405
BstC8I GCNNGC 2 cut(s) 146, 169
BstDEI CTNAG 1 cut(s) 284
BstF5I GGATG 1 cut(s) 269
BstKTI GATC 6 cut(s) 78, 105, 163, 207, 485, 539
BstMBI GATC 6 cut(s) 75, 102, 160, 204, 482, 536
BstMCI CGRYCG 1 cut(s) 485
BstMWI GCNNNNNNNGC 3 cut(s) 236, 503, 512
BstNI CCWGG 1 cut(s) 215
BstSCI CCNGG 2 cut(s) 213, 431
BstV1I GCAGC 3 cut(s) 135, 248, 499
BstX2I RGATCY 1 cut(s) 102
BstYI RGATCY 1 cut(s) 102
BsuI GTATCC 1 cut(s) 86
BsuRI GGCC 1 cut(s) 383
BtsCI GGATG 1 cut(s) 269
Cac8I GCNNGC 2 cut(s) 146, 169
Cfr10I RCCGGY 2 cut(s) 487, 497
Cfr13I GGNCC 1 cut(s) 382
CseI GACGC 4 cut(s) 143, 184, 253, 394
Csp6I GTAC 2 cut(s) 352, 532
CviAII CATG 2 cut(s) 85, 291
CviJI RGCY 7 cut(s) 144, 167, 288, 330, 336, 383, 494
CviKI_1 RGCY 7 cut(s) 144, 167, 288, 330, 336, 383, 494
CviQI GTAC 2 cut(s) 352, 532
DdeI CTNAG 1 cut(s) 284
DpnI GATC 6 cut(s) 77, 104, 162, 206, 484, 538
DpnII GATC 6 cut(s) 75, 102, 160, 204, 482, 536
DriI GACNNNNNGTC 1 cut(s) 441
Eam1104I CTCTTC 2 cut(s) 461, 467
Eam1105I GACNNNNNGTC 1 cut(s) 441
EarI CTCTTC 2 cut(s) 461, 467
Ecl136II GAGCTC 1 cut(s) 144
Eco130I CCWWGG 1 cut(s) 117
Eco24I GRGCYC 2 cut(s) 146, 332
Eco53kI GAGCTC 1 cut(s) 144
EcoICRI GAGCTC 1 cut(s) 144
EcoRII CCWGG 1 cut(s) 213
EcoT14I CCWWGG 1 cut(s) 117
EcoT38I GRGCYC 2 cut(s) 146, 332
ErhI CCWWGG 1 cut(s) 117
FaeI CATG 2 cut(s) 88, 294
FaiI YATR 2 cut(s) 86, 292
FalI AAGNNNNNCTT 1 cut(s) 35
FaqI GGGAC 1 cut(s) 448
FatI CATG 2 cut(s) 84, 290
FbaI TGATCA 1 cut(s) 204
Fnu4HI GCNGC 5 cut(s) 149, 152, 262, 495, 513
FokI GGATG 1 cut(s) 256
FriOI GRGCYC 2 cut(s) 146, 332
Fsp4HI GCNGC 5 cut(s) 149, 152, 262, 495, 513
FspBI CTAG 1 cut(s) 8
GluI GCNGC 5 cut(s) 149, 152, 262, 495, 513
GsaI CCCAGC 1 cut(s) 336
GsuI CTGGAG 1 cut(s) 236
HaeIII GGCC 1 cut(s) 383
HapII CCGG 4 cut(s) 164, 432, 488, 498
HgaI GACGC 4 cut(s) 143, 184, 253, 394
Hin1I GRCGYC 4 cut(s) 135, 222, 342, 405
Hin1II CATG 2 cut(s) 88, 294
HinfI GANTC 2 cut(s) 4, 521
HpaII CCGG 4 cut(s) 164, 432, 488, 498
HphI GGTGA 3 cut(s) 46, 199, 363
Hpy188I TCNGA 1 cut(s) 75
Hpy188III TCNNGA 3 cut(s) 8, 446, 518
Hpy99I CGWCG 9 cut(s) 62, 137, 178, 227, 230, 278, 344, 347, 410
HpyAV CCTTC 2 cut(s) 29, 247
HpyCH4III ACNGT 1 cut(s) 58
HpyCH4IV ACGT 3 cut(s) 124, 222, 342
HpyCH4V TGCA 3 cut(s) 88, 358, 393
HpyF10VI GCNNNNNNNGC 3 cut(s) 236, 503, 512
HpyF3I CTNAG 1 cut(s) 284
HpySE526I ACGT 3 cut(s) 124, 222, 342
Hsp92I GRCGYC 4 cut(s) 135, 222, 342, 405
Hsp92II CATG 2 cut(s) 88, 294
Ksp22I TGATCA 1 cut(s) 204
Kzo9I GATC 6 cut(s) 75, 102, 160, 204, 482, 536
LmnI GCTCC 1 cut(s) 421
Lsp1109I GCAGC 3 cut(s) 135, 248, 499
MaeI CTAG 1 cut(s) 8
MaeII ACGT 3 cut(s) 124, 222, 342
MaeIII GTNAC 1 cut(s) 265
MalI GATC 6 cut(s) 77, 104, 162, 206, 484, 538
MboI GATC 6 cut(s) 75, 102, 160, 204, 482, 536
MboII GAAGA 5 cut(s) 23, 112, 463, 478, 484
MflI RGATCY 1 cut(s) 102
MhlI GDGCHC 2 cut(s) 146, 332
MlyI GAGTC 1 cut(s) 515
MmeI TCCRAC 1 cut(s) 60
MnlI CCTC 8 cut(s) 35, 56, 211, 245, 254, 316, 462, 468
MslI CAYNNNNRTG 1 cut(s) 83
MspA1I CMGCKG 1 cut(s) 154
MspI CCGG 4 cut(s) 164, 432, 488, 498
MspR9I CCNGG 2 cut(s) 215, 433
MvaI CCWGG 1 cut(s) 215
MwoI GCNNNNNNNGC 3 cut(s) 236, 503, 512
NciI CCSGG 1 cut(s) 433
NdeII GATC 6 cut(s) 75, 102, 160, 204, 482, 536
NlaIII CATG 2 cut(s) 88, 294
NlaIV GGNNCC 1 cut(s) 462
NmuCI GTSAC 1 cut(s) 265
PcsI WCGNNNNNNNCGW 2 cut(s) 228, 536
Pfl23II CGTACG 1 cut(s) 531
PflFI GACNNNGTC 2 cut(s) 224, 344
PfoI TCCNGGA 1 cut(s) 431
PkrI GCNGC 5 cut(s) 150, 153, 263, 496, 514
Ple19I CGATCG 1 cut(s) 485
PleI GAGTC 1 cut(s) 515
PpsI GAGTC 1 cut(s) 515
Psp124BI GAGCTC 1 cut(s) 146
Psp6I CCWGG 1 cut(s) 213
PspFI CCCAGC 1 cut(s) 332
PspGI CCWGG 1 cut(s) 213
PspLI CGTACG 1 cut(s) 531
PspN4I GGNNCC 1 cut(s) 462
PspPI GGNCC 1 cut(s) 382
PsuI RGATCY 1 cut(s) 102
PsyI GACNNNGTC 2 cut(s) 224, 344
PvuI CGATCG 1 cut(s) 485
RgaI GCGATCGC 1 cut(s) 485
RsaI GTAC 2 cut(s) 353, 533
RsaNI GTAC 2 cut(s) 352, 532
RseI CAYNNNNRTG 1 cut(s) 83
SacI GAGCTC 1 cut(s) 146
SatI GCNGC 5 cut(s) 149, 152, 262, 495, 513
Sau3AI GATC 6 cut(s) 75, 102, 160, 204, 482, 536
Sau96I GGNCC 1 cut(s) 382
ScaI AGTACT 1 cut(s) 353
SchI GAGTC 1 cut(s) 515
ScrFI CCNGG 2 cut(s) 215, 433
SduI GDGCHC 2 cut(s) 146, 332
SfaAI GCGATCGC 1 cut(s) 485
SgfI GCGATCGC 1 cut(s) 485
SmiMI CAYNNNNRTG 1 cut(s) 83
SsiI CCGC 3 cut(s) 152, 374, 495
SspMI CTAG 1 cut(s) 8
SstI GAGCTC 1 cut(s) 146
StyD4I CCNGG 2 cut(s) 213, 431
StyI CCWWGG 1 cut(s) 117
TaaI ACNGT 1 cut(s) 58
TaiI ACGT 3 cut(s) 127, 225, 345
TaqI TCGA 3 cut(s) 159, 173, 276
TatI WGTACW 1 cut(s) 351
TauI GCSGC 2 cut(s) 154, 497
TseFI GTSAC 1 cut(s) 265
TseI GCWGC 3 cut(s) 148, 261, 512
Tsp45I GTSAC 1 cut(s) 265
TspGWI ACGGA 2 cut(s) 105, 262
Tth111I GACNNNGTC 2 cut(s) 224, 344
XbaI TCTAGA 1 cut(s) 7
XspI CTAG 1 cut(s) 8
ZraI GACGTC 2 cut(s) 223, 343
ZrmI AGTACT 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.