pycom17g26350

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0004545
Physical Location & Seq
Forward (+)
24650038 .. 24652506
2469 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1371 bp
ATGAAGAGGAAAAGAAAATCTGCTGCTTGTGACACCCAAATCCAAATAGGCAACATGGATGATCAAATTCAAGAAAAGGGTCCCCTTATCAGAGATCTTCCGAAGCCCGTCCTTTGGAATATTTTACTAAAGTTACCAACCAGGAGCATTCTCATTTGCAAATGTGTTTGCAAGACTTGGTGTGCTCGAATTTCGGACCCTGAATTTGCTAAGCTGCACTTTGCACAGGCAGAGCCCTGTCCTCTGCTGTCGCTCCTTGGTCCAACTCCAACTCGTGTGCCAAGAACCCTTTACATGGTTGAGCCCGAAGATCGCTCTGGGTTTGATTTGAAGGATTGTGCCTGTGCTGCTACTGGCAATGCTTGTGACCAGTCTCATGTCCACATGAAGCTTACCAAGTACAAGATCCCATTGCGCAACGCTGAGGAGGTCATCAATAGCAACAGTGATAACGGCAATGTGATGCCTAATGGGAACCGCGGCACCAAAAGGAAGACTTGCATCAGGATACCGCCACATCATCACAAGTACAATGTGGTGAATTCGTGTAATGGCCTGCTTTGCTTGTCTGAGCCAGTCCACAATGACCCTGCTGTTGTGTGCAATCCAGTAACCGGTGAATTTATAAACCTTCCCAAAAGTTCTAGGCCGCATCAGAATATAAGGATTCCTGTCGAATGTGGATTGGGTTTCAGTTCCGAGACCAATGAATATAAGGTGATACGAATTTTTGAAGCTGAAGCAGGCTATTGCTTTAAGTTTGCTGAGATACACACACTTGGAACAGGTTCATGGAAGTCCATTGGTGAAGTTTCTGAAACAGCAGGTAACTTAGTATCCCCTACCTATGCGAAAGGAGTGCTTTATTGGTATGAGTCTTATGGCTATAGAATTCGTTCTTTTGATTTGAACACTGAAAAGTTCAAATCAGTTCCATCACCACCTTTTCCGATCAAGCAATTTATGTATGTGGGCATGGGAGTTTTGGAAGGTTGTCTTTGCTTGTGTGATTCCTATGGTGATCGTATCACTGTGTGGACACTGACAGATTCTGGTGGAAAATATCGTTGGACAAAGGTGATTGTTATTGATAAAGGTGATTGTGGAAGGTGGCCATATGGCGTATATACAGCTATGAAATACCATAAGAACTATGGTTTGTTTATGTTTCATTCTCAAACAAATTCCTTCTTTTACTATCACCCAGAAAAACCAAGTTCATACGTTTATCTTAAGCTTTGTGGCTTCAAGGCAAATTTTCAAGCAATTAGTCATGTTCCGAGCTTTATTTTACTCAAGGACAATCTGGCAGGGAAGGATGTAGAAGTATTGAATATAAATACAAGGGTTGAAACTGCAAGGAGAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

457

Amino Acids

51.53

Weight (kDa)

8.91

Isoelectric Point (pI)

44.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 30 - 68 8e-07 F-box-like
F-box PF00646 31 - 69 3.7e-07 F-box domain
Beta-prop_KIB1-4 PF03478 167 - 334 1.5e-07 KIB1-4 beta-propeller
FBA_3 PF08268 168 - 404 2.5e-21 F-box associated beta propeller domain
FBA_1 PF07734 174 - 362 7.7e-18 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 626
Acc16I TGCGCA 1 cut(s) 416
Acc36I ACCTGC 1 cut(s) 815
AccB1I GGYRCC 1 cut(s) 482
AccII CGCG 1 cut(s) 480
AciI CCGC 4 cut(s) 478, 480, 512, 650
AclWI GGATC 1 cut(s) 400
AcoI YGGCCR 1 cut(s) 1112
AcsI RAATTY 9 cut(s) 66, 189, 203, 541, 620, 726, 891, 1183, 1255
AcuI CTGAAG 1 cut(s) 759
AdeI CACNNNGTG 1 cut(s) 1035
AfaI GTAC 2 cut(s) 401, 530
AfiI CCNNNNNNNGG 3 cut(s) 114, 295, 614
AflII CTTAAG 1 cut(s) 1232
AgeI ACCGGT 1 cut(s) 614
AgsI TTSAA 9 cut(s) 71, 331, 734, 910, 925, 1249, 1262, 1333, 1352
AjnI CCWGG 1 cut(s) 140
AjuI GAANNNNNNNTTGG 2 cut(s) 1051, 1083
AluBI AGCT 6 cut(s) 214, 391, 737, 1133, 1237, 1284
AluI AGCT 6 cut(s) 214, 391, 737, 1133, 1237, 1284
Alw21I GWGCWC 1 cut(s) 187
Alw26I GTCTC 3 cut(s) 378, 695, 1359
AlwI GGATC 1 cut(s) 400
AlwNI CAGNNNCTG 1 cut(s) 1052
AoxI GGCC 3 cut(s) 553, 647, 1112
ApeKI GCWGC 3 cut(s) 23, 214, 347
ApoI RAATTY 9 cut(s) 66, 189, 203, 541, 620, 726, 891, 1183, 1255
ArsI GACNNNNNNTTYG 2 cut(s) 188, 220
AsiGI ACCGGT 1 cut(s) 614
Asp700I GAANNNNTTC 2 cut(s) 787, 895
AspLEI GCGC 1 cut(s) 417
AspS9I GGNCC 3 cut(s) 80, 196, 260
AsuHPI GGTGA 9 cut(s) 550, 629, 730, 818, 930, 1031, 1090, 1109, 1193
AvaII GGWCC 3 cut(s) 80, 196, 260
BalI TGGCCA 1 cut(s) 1114
BanI GGYRCC 1 cut(s) 482
BanII GRGCYC 2 cut(s) 237, 306
BauI CACGAG 1 cut(s) 273
BbsI GAAGAC 1 cut(s) 500
Bbv12I GWGCWC 1 cut(s) 187
BbvCI CCTCAGC 1 cut(s) 423
BbvI GCAGC 3 cut(s) 10, 201, 334
BccI CCATC 1 cut(s) 943
BceAI ACGGC 1 cut(s) 469
BcgI CGANNNNNNTGC 2 cut(s) 841, 875
BciT130I CCWGG 1 cut(s) 142
BciVI GTATCC 2 cut(s) 501, 847
BclI TGATCA 1 cut(s) 61
BcoDI GTCTC 3 cut(s) 378, 695, 1359
BfaI CTAG 1 cut(s) 645
BfmI CTRYAG 1 cut(s) 886
BfrI CTTAAG 1 cut(s) 1232
BfuAI ACCTGC 1 cut(s) 815
BfuI GTATCC 2 cut(s) 501, 847
BglII AGATCT 1 cut(s) 94
BisI GCNGC 5 cut(s) 24, 215, 348, 481, 650
BlpI GCTNAGC 1 cut(s) 210
BlsI GCNGC 5 cut(s) 25, 216, 349, 482, 651
Bme1390I CCNGG 1 cut(s) 142
Bme18I GGWCC 3 cut(s) 80, 196, 260
BmgT120I GGNCC 3 cut(s) 80, 196, 260
BmiI GGNNCC 5 cut(s) 81, 82, 198, 476, 484
BmrFI CCNGG 1 cut(s) 142
BmsI GCATC 3 cut(s) 453, 510, 661
BpiI GAAGAC 1 cut(s) 500
Bpu10I CCTNAGC 1 cut(s) 423
Bpu1102I GCTNAGC 1 cut(s) 210
BpuEI CTTGAG 1 cut(s) 1280
BsaI GGTCTC 1 cut(s) 695
BsaJI CCNNGG 2 cut(s) 256, 478
BsaWI WCCGGW 1 cut(s) 614
Bsc4I CCNNNNNNNGG 3 cut(s) 114, 295, 614
Bse118I RCCGGY 1 cut(s) 614
Bse1I ACTGG 4 cut(s) 358, 370, 575, 608
Bse3DI GCAATG 3 cut(s) 364, 410, 463
BseBI CCWGG 1 cut(s) 142
BseDI CCNNGG 2 cut(s) 256, 478
BseGI GGATG 2 cut(s) 64, 1324
BseLI CCNNNNNNNGG 3 cut(s) 114, 295, 614
BseMI GCAATG 3 cut(s) 364, 410, 463
BseMII CTCAG 3 cut(s) 414, 561, 756
BseNI ACTGG 4 cut(s) 358, 370, 575, 608
BseRI GAGGAG 1 cut(s) 440
BseXI GCAGC 3 cut(s) 10, 201, 334
BsgI GTGCAG 1 cut(s) 200
Bsh1236I CGCG 1 cut(s) 480
BshFI GGCC 3 cut(s) 555, 649, 1114
BshNI GGYRCC 1 cut(s) 482
BshTI ACCGGT 1 cut(s) 614
BsiHKAI GWGCWC 1 cut(s) 187
BsiSI CCGG 1 cut(s) 615
BslFI GGGAC 1 cut(s) 66
BslI CCNNNNNNNGG 3 cut(s) 114, 295, 614
BsmAI GTCTC 3 cut(s) 378, 695, 1359
BsmFI GGGAC 1 cut(s) 66
BsmI GAATGC 1 cut(s) 147
BsnI GGCC 3 cut(s) 555, 649, 1114
Bso31I GGTCTC 1 cut(s) 695
Bsp1286I GDGCHC 3 cut(s) 187, 237, 306
Bsp143I GATC 6 cut(s) 61, 94, 310, 405, 951, 1021
Bsp1720I GCTNAGC 1 cut(s) 210
BspACI CCGC 4 cut(s) 478, 480, 512, 650
BspANI GGCC 3 cut(s) 555, 649, 1114
BspCNI CTCAG 3 cut(s) 415, 562, 757
BspFNI CGCG 1 cut(s) 480
BspLI GGNNCC 5 cut(s) 81, 82, 198, 476, 484
BspMI ACCTGC 1 cut(s) 815
BspPI GGATC 1 cut(s) 400
BspT107I GGYRCC 1 cut(s) 482
BspTI CTTAAG 1 cut(s) 1232
BspTNI GGTCTC 1 cut(s) 695
BsrDI GCAATG 3 cut(s) 364, 410, 463
BsrFI RCCGGY 1 cut(s) 614
BsrI ACTGG 4 cut(s) 358, 370, 575, 608
BssAI RCCGGY 1 cut(s) 614
BssECI CCNNGG 2 cut(s) 256, 478
BssMI GATC 6 cut(s) 61, 94, 310, 405, 951, 1021
BssSI CACGAG 1 cut(s) 273
BssT1I CCWWGG 1 cut(s) 256
Bst2BI CACGAG 1 cut(s) 273
Bst2UI CCWGG 1 cut(s) 142
Bst4CI ACNGT 2 cut(s) 446, 1033
BstAFI CTTAAG 1 cut(s) 1232
BstC8I GCNNGC 2 cut(s) 557, 745
BstDEI CTNAG 5 cut(s) 210, 423, 570, 765, 832
BstDSI CCRYGG 1 cut(s) 478
BstF5I GGATG 2 cut(s) 64, 1324
BstFNI CGCG 1 cut(s) 480
BstHHI GCGC 1 cut(s) 417
BstKTI GATC 6 cut(s) 64, 97, 313, 408, 954, 1024
BstMAI GTCTC 3 cut(s) 378, 695, 1359
BstMBI GATC 6 cut(s) 61, 94, 310, 405, 951, 1021
BstMWI GCNNNNNNNGC 2 cut(s) 347, 561
BstNI CCWGG 1 cut(s) 142
BstSCI CCNGG 1 cut(s) 140
BstSFI CTRYAG 1 cut(s) 886
BstUI CGCG 1 cut(s) 480
BstV1I GCAGC 3 cut(s) 10, 201, 334
BstV2I GAAGAC 1 cut(s) 500
BstX2I RGATCY 2 cut(s) 94, 405
BstYI RGATCY 2 cut(s) 94, 405
BsuI GTATCC 2 cut(s) 501, 847
BsuRI GGCC 3 cut(s) 555, 649, 1114
BtgI CCRYGG 1 cut(s) 478
BtsCI GGATG 2 cut(s) 64, 1324
BtsIMutI CAGTG 4 cut(s) 451, 912, 1029, 1040
BveI ACCTGC 1 cut(s) 815
Cac8I GCNNGC 2 cut(s) 557, 745
CaiI CAGNNNCTG 1 cut(s) 1052
CfoI GCGC 1 cut(s) 417
Cfr10I RCCGGY 1 cut(s) 614
Cfr13I GGNCC 3 cut(s) 80, 196, 260
Cfr42I CCGCGG 1 cut(s) 481
Csp6I GTAC 2 cut(s) 400, 529
CspAI ACCGGT 1 cut(s) 614
CviAII CATG 7 cut(s) 55, 295, 377, 385, 792, 976, 1274
CviQI GTAC 2 cut(s) 400, 529
DdeI CTNAG 5 cut(s) 210, 423, 570, 765, 832
DpnI GATC 6 cut(s) 63, 96, 312, 407, 953, 1023
DpnII GATC 6 cut(s) 61, 94, 310, 405, 951, 1021
DraIII CACNNNGTG 1 cut(s) 1035
EaeI YGGCCR 1 cut(s) 1112
Eco130I CCWWGG 1 cut(s) 256
Eco24I GRGCYC 2 cut(s) 237, 306
Eco31I GGTCTC 1 cut(s) 695
Eco47I GGWCC 3 cut(s) 80, 196, 260
Eco57I CTGAAG 1 cut(s) 759
EcoO109I RGGNCCY 1 cut(s) 80
EcoRI GAATTC 2 cut(s) 541, 891
EcoRII CCWGG 1 cut(s) 140
EcoT14I CCWWGG 1 cut(s) 256
EcoT38I GRGCYC 2 cut(s) 237, 306
ErhI CCWWGG 1 cut(s) 256
FaeI CATG 7 cut(s) 58, 298, 380, 388, 795, 979, 1277
FalI AAGNNNNNCTT 8 cut(s) 203, 235, 481, 513, 846, 878, 981, 1013
FaqI GGGAC 1 cut(s) 66
FatI CATG 7 cut(s) 54, 294, 376, 384, 791, 975, 1273
FauNDI CATATG 1 cut(s) 1117
FbaI TGATCA 1 cut(s) 61
Fnu4HI GCNGC 5 cut(s) 24, 215, 348, 481, 650
FokI GGATG 2 cut(s) 71, 1331
FriOI GRGCYC 2 cut(s) 237, 306
Fsp4HI GCNGC 5 cut(s) 24, 215, 348, 481, 650
FspBI CTAG 1 cut(s) 645
FspI TGCGCA 1 cut(s) 416
GlaI GCGC 1 cut(s) 416
GluI GCNGC 5 cut(s) 24, 215, 348, 481, 650
HaeIII GGCC 3 cut(s) 555, 649, 1114
HapII CCGG 1 cut(s) 615
HhaI GCGC 1 cut(s) 417
Hin1II CATG 7 cut(s) 58, 298, 380, 388, 795, 979, 1277
Hin6I GCGC 1 cut(s) 415
HinP1I GCGC 1 cut(s) 415
HindIII AAGCTT 2 cut(s) 389, 1235
HinfI GANTC 4 cut(s) 667, 875, 1010, 1049
HpaII CCGG 1 cut(s) 615
HphI GGTGA 9 cut(s) 550, 629, 730, 818, 930, 1031, 1090, 1109, 1193
Hpy166II GTNNAC 3 cut(s) 382, 580, 1038
Hpy188I TCNGA 9 cut(s) 92, 102, 196, 571, 657, 700, 817, 951, 1281
Hpy188III TCNNGA 2 cut(s) 71, 505
Hpy8I GTNNAC 3 cut(s) 382, 580, 1038
HpyAV CCTTC 6 cut(s) 325, 641, 983, 1101, 1198, 1309
HpyCH4III ACNGT 2 cut(s) 446, 1033
HpyCH4IV ACGT 1 cut(s) 1224
HpyCH4V TGCA 7 cut(s) 159, 171, 217, 224, 501, 603, 1358
HpyF10VI GCNNNNNNNGC 2 cut(s) 347, 561
HpyF3I CTNAG 5 cut(s) 210, 423, 570, 765, 832
HpySE526I ACGT 1 cut(s) 1224
Hsp92II CATG 7 cut(s) 58, 298, 380, 388, 795, 979, 1277
HspAI GCGC 1 cut(s) 415
KflI GGGWCCC 1 cut(s) 80
Ksp22I TGATCA 1 cut(s) 61
KspI CCGCGG 1 cut(s) 481
Kzo9I GATC 6 cut(s) 61, 94, 310, 405, 951, 1021
LmnI GCTCC 2 cut(s) 144, 258
Lsp1109I GCAGC 3 cut(s) 10, 201, 334
LweI GCATC 3 cut(s) 453, 510, 661
MaeI CTAG 1 cut(s) 645
MaeII ACGT 1 cut(s) 1224
MaeIII GTNAC 5 cut(s) 29, 132, 365, 610, 827
MalI GATC 6 cut(s) 63, 96, 312, 407, 953, 1023
MboI GATC 6 cut(s) 61, 94, 310, 405, 951, 1021
MboII GAAGA 4 cut(s) 16, 89, 320, 505
MflI RGATCY 2 cut(s) 94, 405
MhlI GDGCHC 3 cut(s) 187, 237, 306
MlsI TGGCCA 1 cut(s) 1114
MluNI TGGCCA 1 cut(s) 1114
MlyI GAGTC 1 cut(s) 884
MmeI TCCRAC 3 cut(s) 287, 293, 1049
MnlI CCTC 3 cut(s) 252, 418, 421
Mox20I TGGCCA 1 cut(s) 1114
MroXI GAANNNNTTC 2 cut(s) 787, 895
MscI TGGCCA 1 cut(s) 1114
MseI TTAA 2 cut(s) 756, 1233
Msp20I TGGCCA 1 cut(s) 1114
MspA1I CMGCKG 1 cut(s) 480
MspCI CTTAAG 1 cut(s) 1232
MspI CCGG 1 cut(s) 615
MspR9I CCNGG 1 cut(s) 142
Mva1269I GAATGC 1 cut(s) 147
MvaI CCWGG 1 cut(s) 142
MvnI CGCG 1 cut(s) 480
MwoI GCNNNNNNNGC 2 cut(s) 347, 561
NdeI CATATG 1 cut(s) 1117
NdeII GATC 6 cut(s) 61, 94, 310, 405, 951, 1021
NlaIII CATG 7 cut(s) 58, 298, 380, 388, 795, 979, 1277
NlaIV GGNNCC 5 cut(s) 81, 82, 198, 476, 484
NmuCI GTSAC 2 cut(s) 29, 365
NsbI TGCGCA 1 cut(s) 416
PctI GAATGC 1 cut(s) 147
PdmI GAANNNNTTC 2 cut(s) 787, 895
PfeI GAWTC 3 cut(s) 667, 1010, 1049
PinAI ACCGGT 1 cut(s) 614
PkrI GCNGC 5 cut(s) 25, 216, 349, 482, 651
PleI GAGTC 1 cut(s) 883
PpsI GAGTC 1 cut(s) 883
PpuMI RGGWCCY 1 cut(s) 80
PsiI TTATAA 1 cut(s) 626
Psp5II RGGWCCY 1 cut(s) 80
Psp6I CCWGG 1 cut(s) 140
PspGI CCWGG 1 cut(s) 140
PspN4I GGNNCC 5 cut(s) 81, 82, 198, 476, 484
PspPI GGNCC 3 cut(s) 80, 196, 260
PspPPI RGGWCCY 1 cut(s) 80
PstNI CAGNNNCTG 1 cut(s) 1052
PsuI RGATCY 2 cut(s) 94, 405
RsaI GTAC 2 cut(s) 401, 530
RsaNI GTAC 2 cut(s) 400, 529
SacII CCGCGG 1 cut(s) 481
SaqAI TTAA 2 cut(s) 756, 1233
SatI GCNGC 5 cut(s) 24, 215, 348, 481, 650
Sau3AI GATC 6 cut(s) 61, 94, 310, 405, 951, 1021
Sau96I GGNCC 3 cut(s) 80, 196, 260
SchI GAGTC 1 cut(s) 884
ScrFI CCNGG 1 cut(s) 142
SduI GDGCHC 3 cut(s) 187, 237, 306
SfaNI GCATC 3 cut(s) 453, 510, 661
SfcI CTRYAG 1 cut(s) 886
Sfr303I CCGCGG 1 cut(s) 481
SgrBI CCGCGG 1 cut(s) 481
SinI GGWCC 3 cut(s) 80, 196, 260
SmlI CTYRAG 2 cut(s) 1232, 1295
SmoI CTYRAG 2 cut(s) 1232, 1295
SsiI CCGC 4 cut(s) 478, 480, 512, 650
SspI AATATT 1 cut(s) 121
SspMI CTAG 1 cut(s) 645
StyD4I CCNGG 1 cut(s) 140
StyI CCWWGG 1 cut(s) 256
TaaI ACNGT 2 cut(s) 446, 1033
TaiI ACGT 1 cut(s) 1227
TaqI TCGA 2 cut(s) 187, 675
TatI WGTACW 2 cut(s) 399, 528
TauI GCSGC 2 cut(s) 483, 652
TfiI GAWTC 3 cut(s) 667, 1010, 1049
Tru1I TTAA 2 cut(s) 756, 1233
Tru9I TTAA 2 cut(s) 756, 1233
TscAI CASTG 4 cut(s) 451, 919, 1036, 1047
TseFI GTSAC 2 cut(s) 29, 365
TseI GCWGC 3 cut(s) 23, 214, 347
Tsp45I GTSAC 2 cut(s) 29, 365
TspDTI ATGAA 7 cut(s) 17, 401, 723, 780, 1151, 1160, 1209
TspRI CASTG 4 cut(s) 451, 919, 1036, 1047
Vha464I CTTAAG 1 cut(s) 1232
VpaK11BI GGWCC 3 cut(s) 80, 196, 260
XapI RAATTY 9 cut(s) 66, 189, 203, 541, 620, 726, 891, 1183, 1255
XcmI CCANNNNNNNNNTGG 1 cut(s) 1151
XmnI GAANNNNTTC 2 cut(s) 787, 895
XspI CTAG 1 cut(s) 645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.