pycom49g00020

Belongs to the GDA1 CD39 NTPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_49
OG0001988
Physical Location & Seq
Reverse (-)
29896 .. 32248
2353 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1407 bp
ATGCTCAAGCGTCCCGGCAGAGGAATCGGAATGGGGAGTCCCCAGTGCGACTCCCTCTCCGACAAAATGTACAGATACAGGAGCGTCGTCCTCGTCATCTCAATCCCTCTCCTCCTCCTTACCATGGTCCTTTACGTCATGCCCCCCGGCGGTAATTTGAGCAACCAGCTCCCCCGCAAGCTGCCTTCCAAGACATCCTACGCTGTGATCGTCGACGCCGGCAGCTCCGGCAGCCGCGTCCACGTCTTCTGCTTCGACCACAACCTCCAGCTCCTTCCCATTGGCAAAGATCTCGAGCTCTTTCTTCAGGTCAAGCCTGGTCTGAGCGCTTATGCCAAGGACCCTCGCTCTGCTGCGGAGTCCCTCACCGGTCTTCTCGATAAGGCTGAGAACGCAGTTCCCAAGGAGCTTCGTTCCTTAACTCTCGTCCGCGTTGGCGCTACGGCTGGCCTTAGGGCATTGGAGGGCGATGCTTCTGACCGCATTCTCCAGGCCGTCAGGGATCTGCTCAAACAAAGAAGTAGCCTCAGCTCCCGCCCTGATGCCGTTACTGTCATTGATGGAACTCAAGAAGGTTCCTTCCAGTGGGTGACAATAAACTATCTACTAGGAAATTTGGGGAAGAAATATTCAGATACTGTAGGGGTGGTTGACCTTGGAGGTGGATCTGTGCAGATGGCTTATGCTATCTCAGAGGCAGATGCTGCAAAAGCACCCAAGTTATCAGATGGAGAGGATGTATATGTAAGGCAAATGAATCTTAAGGGAGTTAAATATAACCTTTATGTTCACAGTTACTTGCATTACGGGTTACTAGCAGCTCGAGCAGAGATTTTAAAGGTTTCTGAAGATTCTGGCAACCCCTGCATCTTAGCCGGATATTATGGTTCTTACGCATATGGAGGACAATCTTATAAAGCATCAGCTTCCTCATCTGGATCAAGTGTTGAGGAGTGCAGGAGGGTAGTTACAAAGGCACTCAAAGTAAATGAAGCAACATGTTCACACATGAAGTGCACATTTGGCGGGGTATGGAACGGTGGAGGAGGGGATGGACAAAGGAACCTATTTGTGGCTTCATTTTTCTTTGACAGAGCTGCTGAGGCTGGATTTGTTAACCCAAATGAACCTGTTGCGAAAGTTCACCCTGGTAACTTTGAGGAGGCAGCTAAGCGTGCTTGTGAAACTAAACTTGCGGATGCTAAATCAACATATCCAAGAGTTGAGGAGGGTAACCTGCCGTTCTTGTGCATGGATCTTGTTTACCAATCTACATTGCTTGTGGATGGATTTGGTCTTGATCCCTGGCAAGAGATTACATTGGTGAAGAAAGTTAAATATCAAAATTCCTTGGTTGAAGCAGCTTGGCCACTGGGGAGTGCCATAGAGGCCGTGTCCTCATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 map00230 map00240
Pfam Domains
Protein Families
GDA1_CD39

Protein Analysis

469

Amino Acids

50.46

Weight (kDa)

8.25

Isoelectric Point (pI)

41.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GDA1_CD39 PF01150 63 - 466 2.5e-105 GDA1/CD39 (nucleoside phosphatase) family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 915, 1405
Acc36I ACCTGC 1 cut(s) 1245
AccI GTMKAC 1 cut(s) 213
AccII CGCG 2 cut(s) 237, 432
AciI CCGC 9 cut(s) 150, 175, 235, 356, 430, 481, 535, 1026, 1196
AclWI GGATC 5 cut(s) 510, 673, 946, 1263, 1295
AcoI YGGCCR 1 cut(s) 1367
AcsI RAATTY 2 cut(s) 613, 1345
AcuI CTGAAG 2 cut(s) 290, 867
AcyI GRCGYC 1 cut(s) 216
AfaI GTAC 1 cut(s) 71
AfeI AGCGCT 1 cut(s) 328
AfiI CCNNNNNNNGG 5 cut(s) 20, 124, 149, 585, 1072
AflII CTTAAG 1 cut(s) 761
AflIII ACRYGT 1 cut(s) 998
AgeI ACCGGT 1 cut(s) 368
AgsI TTSAA 1 cut(s) 1358
AjiI CACGTC 1 cut(s) 244
AjnI CCWGG 4 cut(s) 316, 489, 1147, 1304
Alw21I GWGCWC 2 cut(s) 300, 1019
Alw44I GTGCAC 1 cut(s) 1015
AlwI GGATC 5 cut(s) 510, 673, 946, 1263, 1295
AlwNI CAGNNNCTG 2 cut(s) 638, 704
Ama87I CYCGRG 2 cut(s) 293, 822
Aor51HI AGCGCT 1 cut(s) 328
AoxI GGCC 4 cut(s) 448, 492, 1367, 1389
ApaLI GTGCAC 1 cut(s) 1015
ApeKI GCWGC 9 cut(s) 181, 222, 231, 353, 704, 818, 1097, 1166, 1361
ApoI RAATTY 2 cut(s) 613, 1345
AsiGI ACCGGT 1 cut(s) 368
AspLEI GCGC 2 cut(s) 329, 440
AspS9I GGNCC 2 cut(s) 127, 340
AsuC2I CCSGG 2 cut(s) 15, 147
AsuHPI GGTGA 4 cut(s) 358, 601, 1136, 1336
AvaI CYCGRG 2 cut(s) 293, 822
AvaII GGWCC 2 cut(s) 127, 340
AxyI CCTNAGG 1 cut(s) 452
BaeGI GKGCMC 1 cut(s) 1019
BalI TGGCCA 1 cut(s) 1369
BanII GRGCYC 1 cut(s) 300
BbsI GAAGAC 2 cut(s) 238, 365
Bbv12I GWGCWC 2 cut(s) 300, 1019
BbvCI CCTCAGC 2 cut(s) 527, 1101
BbvI GCAGC 9 cut(s) 168, 234, 243, 340, 691, 830, 1084, 1178, 1373
BccI CCATC 5 cut(s) 554, 670, 722, 1046, 1280
BceAI ACGGC 5 cut(s) 459, 479, 530, 1225, 1376
BcgI CGANNNNNNTGC 4 cut(s) 7, 41, 274, 308
BciT130I CCWGG 4 cut(s) 318, 491, 1149, 1306
BcnI CCSGG 2 cut(s) 15, 147
BfaI CTAG 2 cut(s) 608, 815
BfmI CTRYAG 1 cut(s) 639
BfoI RGCGCY 2 cut(s) 330, 441
BfrI CTTAAG 1 cut(s) 761
BfuAI ACCTGC 1 cut(s) 1245
BglI GCCNNNNNGGC 1 cut(s) 1388
BglII AGATCT 1 cut(s) 289
BlpI GCTNAGC 1 cut(s) 1170
Bme1390I CCNGG 6 cut(s) 15, 147, 318, 491, 1149, 1306
Bme18I GGWCC 2 cut(s) 127, 340
BmeT110I CYCGRG 2 cut(s) 293, 822
BmgBI CACGTC 1 cut(s) 244
BmgT120I GGNCC 2 cut(s) 127, 340
BmiI GGNNCC 3 cut(s) 342, 577, 1064
BmrFI CCNGG 6 cut(s) 15, 147, 318, 491, 1149, 1306
BmrI ACTGGG 2 cut(s) 37, 1382
BmsI GCATC 6 cut(s) 460, 532, 691, 876, 929, 1189
BmuI ACTGGG 2 cut(s) 37, 1382
BpiI GAAGAC 2 cut(s) 238, 365
BpmI CTGGAG 2 cut(s) 251, 473
Bpu10I CCTNAGC 2 cut(s) 527, 1101
Bpu1102I GCTNAGC 1 cut(s) 1170
BpuEI CTTGAG 1 cut(s) 552
BpuMI CCSGG 2 cut(s) 15, 147
BsaHI GRCGYC 1 cut(s) 216
BsaJI CCNNGG 8 cut(s) 123, 145, 336, 402, 655, 1147, 1304, 1350
BsaWI WCCGGW 1 cut(s) 368
BsaXI ACNNNNNCTCC 4 cut(s) 41, 71, 723, 753
Bsc4I CCNNNNNNNGG 5 cut(s) 20, 124, 149, 585, 1072
Bse118I RCCGGY 2 cut(s) 218, 368
Bse1I ACTGG 3 cut(s) 43, 583, 1377
Bse21I CCTNAGG 1 cut(s) 452
Bse3DI GCAATG 1 cut(s) 1274
BseBI CCWGG 4 cut(s) 318, 491, 1149, 1306
BseDI CCNNGG 8 cut(s) 123, 145, 336, 402, 655, 1147, 1304, 1350
BseGI GGATG 5 cut(s) 194, 742, 1057, 1204, 1291
BseLI CCNNNNNNNGG 5 cut(s) 20, 124, 149, 585, 1072
BseMI GCAATG 1 cut(s) 1274
BseMII CTCAG 5 cut(s) 314, 378, 541, 705, 1092
BseNI ACTGG 3 cut(s) 43, 583, 1377
BseRI GAGGAG 6 cut(s) 101, 104, 965, 1059, 1175, 1241
BseSI GKGCMC 1 cut(s) 1019
BseXI GCAGC 9 cut(s) 168, 234, 243, 340, 691, 830, 1084, 1178, 1373
BsgI GTGCAG 2 cut(s) 692, 976
Bsh1236I CGCG 2 cut(s) 237, 432
BshFI GGCC 4 cut(s) 450, 494, 1369, 1391
BshTI ACCGGT 1 cut(s) 368
BsiHKAI GWGCWC 2 cut(s) 300, 1019
BsiHKCI CYCGRG 2 cut(s) 293, 822
BsiSI CCGG 6 cut(s) 15, 147, 219, 228, 369, 876
BslFI GGGAC 2 cut(s) 24, 346
BslI CCNNNNNNNGG 5 cut(s) 20, 124, 149, 585, 1072
BsmFI GGGAC 2 cut(s) 24, 346
BsmI GAATGC 1 cut(s) 483
BsnI GGCC 4 cut(s) 450, 494, 1369, 1391
BsoBI CYCGRG 2 cut(s) 293, 822
Bsp1286I GDGCHC 2 cut(s) 300, 1019
Bsp1407I TGTACA 1 cut(s) 69
Bsp143I GATC 7 cut(s) 207, 289, 502, 665, 938, 1255, 1300
Bsp1720I GCTNAGC 1 cut(s) 1170
Bsp19I CCATGG 1 cut(s) 123
BspACI CCGC 9 cut(s) 150, 175, 235, 356, 430, 481, 535, 1026, 1196
BspANI GGCC 4 cut(s) 450, 494, 1369, 1391
BspCNI CTCAG 5 cut(s) 315, 379, 540, 704, 1093
BspFNI CGCG 2 cut(s) 237, 432
BspLI GGNNCC 3 cut(s) 342, 577, 1064
BspMI ACCTGC 1 cut(s) 1245
BspPI GGATC 5 cut(s) 510, 673, 946, 1263, 1295
BspTI CTTAAG 1 cut(s) 761
BsrDI GCAATG 1 cut(s) 1274
BsrFI RCCGGY 2 cut(s) 218, 368
BsrGI TGTACA 1 cut(s) 69
BsrI ACTGG 3 cut(s) 43, 583, 1377
BssAI RCCGGY 2 cut(s) 218, 368
BssECI CCNNGG 8 cut(s) 123, 145, 336, 402, 655, 1147, 1304, 1350
BssMI GATC 7 cut(s) 207, 289, 502, 665, 938, 1255, 1300
BssNI GRCGYC 1 cut(s) 216
BssT1I CCWWGG 5 cut(s) 123, 336, 402, 655, 1350
Bst2UI CCWGG 4 cut(s) 318, 491, 1149, 1306
Bst4CI ACNGT 4 cut(s) 553, 640, 794, 1040
BstACI GRCGYC 1 cut(s) 216
BstAFI CTTAAG 1 cut(s) 761
BstAPI GCANNNNNTGC 2 cut(s) 704, 864
BstAUI TGTACA 1 cut(s) 69
BstC8I GCNNGC 4 cut(s) 179, 220, 448, 1176
BstDEI CTNAG 8 cut(s) 323, 387, 452, 527, 691, 871, 1101, 1170
BstDSI CCRYGG 1 cut(s) 123
BstEII GGTNACC 1 cut(s) 1232
BstF5I GGATG 5 cut(s) 194, 742, 1057, 1204, 1291
BstFNI CGCG 2 cut(s) 237, 432
BstH2I RGCGCY 2 cut(s) 330, 441
BstHHI GCGC 2 cut(s) 329, 440
BstKTI GATC 7 cut(s) 210, 292, 505, 668, 941, 1258, 1303
BstMBI GATC 7 cut(s) 207, 289, 502, 665, 938, 1255, 1300
BstNI CCWGG 4 cut(s) 318, 491, 1149, 1306
BstNSI RCATGY 1 cut(s) 1002
BstPI GGTNACC 1 cut(s) 1232
BstSCI CCNGG 6 cut(s) 13, 145, 316, 489, 1147, 1304
BstSFI CTRYAG 1 cut(s) 639
BstSLI GKGCMC 1 cut(s) 1019
BstUI CGCG 2 cut(s) 237, 432
BstV1I GCAGC 9 cut(s) 168, 234, 243, 340, 691, 830, 1084, 1178, 1373
BstV2I GAAGAC 2 cut(s) 238, 365
BstX2I RGATCY 4 cut(s) 289, 502, 665, 1255
BstYI RGATCY 4 cut(s) 289, 502, 665, 1255
Bsu36I CCTNAGG 1 cut(s) 452
BsuRI GGCC 4 cut(s) 450, 494, 1369, 1391
BtgI CCRYGG 1 cut(s) 123
BtgZI GCGATG 1 cut(s) 483
BtrI CACGTC 1 cut(s) 244
BtsCI GGATG 5 cut(s) 194, 742, 1057, 1204, 1291
BtsIMutI CAGTG 3 cut(s) 50, 590, 1370
BveI ACCTGC 1 cut(s) 1245
Cac8I GCNNGC 4 cut(s) 179, 220, 448, 1176
CaiI CAGNNNCTG 2 cut(s) 638, 704
CfoI GCGC 2 cut(s) 329, 440
Cfr10I RCCGGY 2 cut(s) 218, 368
Cfr13I GGNCC 2 cut(s) 127, 340
CseI GACGC 3 cut(s) 73, 224, 226
Csp6I GTAC 1 cut(s) 70
CspAI ACCGGT 1 cut(s) 368
CviAII CATG 5 cut(s) 124, 139, 999, 1009, 1252
CviQI GTAC 1 cut(s) 70
DdeI CTNAG 8 cut(s) 323, 387, 452, 527, 691, 871, 1101, 1170
DpnI GATC 7 cut(s) 209, 291, 504, 667, 940, 1257, 1302
DpnII GATC 7 cut(s) 207, 289, 502, 665, 938, 1255, 1300
DraI TTTAAA 1 cut(s) 837
EaeI YGGCCR 1 cut(s) 1367
Ecl136II GAGCTC 1 cut(s) 298
Eco130I CCWWGG 5 cut(s) 123, 336, 402, 655, 1350
Eco24I GRGCYC 1 cut(s) 300
Eco47I GGWCC 2 cut(s) 127, 340
Eco47III AGCGCT 1 cut(s) 328
Eco53kI GAGCTC 1 cut(s) 298
Eco57I CTGAAG 2 cut(s) 290, 867
Eco81I CCTNAGG 1 cut(s) 452
Eco88I CYCGRG 2 cut(s) 293, 822
Eco91I GGTNACC 1 cut(s) 1232
EcoICRI GAGCTC 1 cut(s) 298
EcoO109I RGGNCCY 1 cut(s) 340
EcoO65I GGTNACC 1 cut(s) 1232
EcoRII CCWGG 4 cut(s) 316, 489, 1147, 1304
EcoT14I CCWWGG 5 cut(s) 123, 336, 402, 655, 1350
EcoT38I GRGCYC 1 cut(s) 300
ErhI CCWWGG 5 cut(s) 123, 336, 402, 655, 1350
FaeI CATG 5 cut(s) 127, 142, 1002, 1012, 1255
FaqI GGGAC 2 cut(s) 24, 346
FatI CATG 5 cut(s) 123, 138, 998, 1008, 1251
FauI CCCGC 3 cut(s) 182, 542, 1019
FauNDI CATATG 1 cut(s) 898
FblI GTMKAC 1 cut(s) 213
FokI GGATG 5 cut(s) 181, 749, 1064, 1211, 1298
FriOI GRGCYC 1 cut(s) 300
FspBI CTAG 2 cut(s) 608, 815
GlaI GCGC 2 cut(s) 328, 439
GsuI CTGGAG 2 cut(s) 251, 473
HaeII RGCGCY 2 cut(s) 330, 441
HaeIII GGCC 4 cut(s) 450, 494, 1369, 1391
HapII CCGG 6 cut(s) 15, 147, 219, 228, 369, 876
HgaI GACGC 3 cut(s) 73, 224, 226
HhaI GCGC 2 cut(s) 329, 440
Hin1I GRCGYC 1 cut(s) 216
Hin1II CATG 5 cut(s) 127, 142, 1002, 1012, 1255
Hin6I GCGC 2 cut(s) 327, 438
HinP1I GCGC 2 cut(s) 327, 438
HincII GTYRAC 3 cut(s) 214, 652, 1117
HindII GTYRAC 3 cut(s) 214, 652, 1117
HinfI GANTC 6 cut(s) 24, 37, 50, 359, 757, 851
HpaI GTTAAC 1 cut(s) 1117
HpaII CCGG 6 cut(s) 15, 147, 219, 228, 369, 876
HphI GGTGA 4 cut(s) 358, 601, 1136, 1336
Hpy166II GTNNAC 9 cut(s) 214, 241, 652, 790, 1004, 1017, 1117, 1144, 1264
Hpy188I TCNGA 8 cut(s) 29, 61, 324, 478, 634, 694, 727, 847
Hpy188III TCNNGA 5 cut(s) 293, 377, 569, 936, 1298
Hpy8I GTNNAC 9 cut(s) 214, 241, 652, 790, 1004, 1017, 1117, 1144, 1264
Hpy99I CGWCG 3 cut(s) 89, 215, 218
HpyAV CCTTC 4 cut(s) 195, 284, 566, 589
HpyCH4III ACNGT 4 cut(s) 553, 640, 794, 1040
HpyCH4IV ACGT 2 cut(s) 135, 243
HpyCH4V TGCA 7 cut(s) 673, 707, 802, 867, 957, 1017, 1251
HpyF3I CTNAG 8 cut(s) 323, 387, 452, 527, 691, 871, 1101, 1170
HpySE526I ACGT 2 cut(s) 135, 243
Hsp92I GRCGYC 1 cut(s) 216
Hsp92II CATG 5 cut(s) 127, 142, 1002, 1012, 1255
HspAI GCGC 2 cut(s) 327, 438
KroI GCCGGC 1 cut(s) 218
KroNI GCCGGC 1 cut(s) 220
KspAI GTTAAC 1 cut(s) 1117
Kzo9I GATC 7 cut(s) 207, 289, 502, 665, 938, 1255, 1300
LmnI GCTCC 6 cut(s) 81, 174, 230, 276, 406, 536
Lsp1109I GCAGC 9 cut(s) 168, 234, 243, 340, 691, 830, 1084, 1178, 1373
LweI GCATC 6 cut(s) 460, 532, 691, 876, 929, 1189
MaeI CTAG 2 cut(s) 608, 815
MaeII ACGT 2 cut(s) 135, 243
MaeIII GTNAC 7 cut(s) 547, 589, 794, 810, 967, 1151, 1232
MalI GATC 7 cut(s) 209, 291, 504, 667, 940, 1257, 1302
MboI GATC 7 cut(s) 207, 289, 502, 665, 938, 1255, 1300
MboII GAAGA 6 cut(s) 238, 296, 365, 634, 860, 1339
MflI RGATCY 4 cut(s) 289, 502, 665, 1255
MhlI GDGCHC 2 cut(s) 300, 1019
MlsI TGGCCA 1 cut(s) 1369
MluCI AATT 3 cut(s) 154, 613, 1345
MluNI TGGCCA 1 cut(s) 1369
MlyI GAGTC 3 cut(s) 44, 46, 368
MmeI TCCRAC 1 cut(s) 84
Mox20I TGGCCA 1 cut(s) 1369
MroNI GCCGGC 1 cut(s) 218
MscI TGGCCA 1 cut(s) 1369
MseI TTAA 6 cut(s) 419, 762, 771, 836, 1116, 1335
Msp20I TGGCCA 1 cut(s) 1369
MspCI CTTAAG 1 cut(s) 761
MspI CCGG 6 cut(s) 15, 147, 219, 228, 369, 876
MspR9I CCNGG 6 cut(s) 15, 147, 318, 491, 1149, 1306
Mva1269I GAATGC 1 cut(s) 483
MvaI CCWGG 4 cut(s) 318, 491, 1149, 1306
MvnI CGCG 2 cut(s) 237, 432
NaeI GCCGGC 1 cut(s) 220
NciI CCSGG 2 cut(s) 15, 147
NcoI CCATGG 1 cut(s) 123
NdeI CATATG 1 cut(s) 898
NdeII GATC 7 cut(s) 207, 289, 502, 665, 938, 1255, 1300
NgoMIV GCCGGC 1 cut(s) 218
NlaIII CATG 5 cut(s) 127, 142, 1002, 1012, 1255
NlaIV GGNNCC 3 cut(s) 342, 577, 1064
NmuCI GTSAC 1 cut(s) 589
NspI RCATGY 1 cut(s) 1002
PaeR7I CTCGAG 2 cut(s) 293, 822
PciI ACATGT 1 cut(s) 998
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PctI GAATGC 1 cut(s) 483
PdiI GCCGGC 1 cut(s) 220
PfeI GAWTC 3 cut(s) 24, 757, 851
PinAI ACCGGT 1 cut(s) 368
PleI GAGTC 3 cut(s) 44, 45, 367
PpsI GAGTC 3 cut(s) 44, 45, 367
PpuMI RGGWCCY 1 cut(s) 340
PscI ACATGT 1 cut(s) 998
PsiI TTATAA 2 cut(s) 915, 1405
Psp124BI GAGCTC 1 cut(s) 300
Psp5II RGGWCCY 1 cut(s) 340
Psp6I CCWGG 4 cut(s) 316, 489, 1147, 1304
PspEI GGTNACC 1 cut(s) 1232
PspGI CCWGG 4 cut(s) 316, 489, 1147, 1304
PspN4I GGNNCC 3 cut(s) 342, 577, 1064
PspPI GGNCC 2 cut(s) 127, 340
PspPPI RGGWCCY 1 cut(s) 340
PspXI VCTCGAGB 1 cut(s) 822
PstNI CAGNNNCTG 2 cut(s) 638, 704
PsuI RGATCY 4 cut(s) 289, 502, 665, 1255
RsaI GTAC 1 cut(s) 71
RsaNI GTAC 1 cut(s) 70
SacI GAGCTC 1 cut(s) 300
SalI GTCGAC 1 cut(s) 212
SaqAI TTAA 6 cut(s) 419, 762, 771, 836, 1116, 1335
Sau3AI GATC 7 cut(s) 207, 289, 502, 665, 938, 1255, 1300
Sau96I GGNCC 2 cut(s) 127, 340
SchI GAGTC 3 cut(s) 44, 46, 368
ScrFI CCNGG 6 cut(s) 15, 147, 318, 491, 1149, 1306
SduI GDGCHC 2 cut(s) 300, 1019
SfaNI GCATC 6 cut(s) 460, 532, 691, 876, 929, 1189
SfcI CTRYAG 1 cut(s) 639
Sfr274I CTCGAG 2 cut(s) 293, 822
SgrDI CGTCGACG 1 cut(s) 212
SinI GGWCC 2 cut(s) 127, 340
SlaI CTCGAG 2 cut(s) 293, 822
SmlI CTYRAG 5 cut(s) 5, 293, 567, 761, 822
SmoI CTYRAG 5 cut(s) 5, 293, 567, 761, 822
Sse9I AATT 3 cut(s) 154, 613, 1345
SsiI CCGC 9 cut(s) 150, 175, 235, 356, 430, 481, 535, 1026, 1196
SspI AATATT 1 cut(s) 629
SspMI CTAG 2 cut(s) 608, 815
SstI GAGCTC 1 cut(s) 300
StyD4I CCNGG 6 cut(s) 13, 145, 316, 489, 1147, 1304
StyI CCWWGG 5 cut(s) 123, 336, 402, 655, 1350
TaaI ACNGT 4 cut(s) 553, 640, 794, 1040
TaiI ACGT 2 cut(s) 138, 246
TaqI TCGA 5 cut(s) 213, 255, 294, 378, 823
TasI AATT 3 cut(s) 154, 613, 1345
TatI WGTACW 1 cut(s) 69
TauI GCSGC 1 cut(s) 237
TfiI GAWTC 3 cut(s) 24, 757, 851
Tru1I TTAA 6 cut(s) 419, 762, 771, 836, 1116, 1335
Tru9I TTAA 6 cut(s) 419, 762, 771, 836, 1116, 1335
TscAI CASTG 3 cut(s) 50, 590, 1377
TseFI GTSAC 1 cut(s) 589
TseI GCWGC 9 cut(s) 181, 222, 231, 353, 704, 818, 1097, 1166, 1361
Tsp45I GTSAC 1 cut(s) 589
TspDTI ATGAA 5 cut(s) 770, 1005, 1025, 1068, 1140
TspRI CASTG 3 cut(s) 50, 590, 1377
Vha464I CTTAAG 1 cut(s) 761
VneI GTGCAC 1 cut(s) 1015
VpaK11BI GGWCC 2 cut(s) 127, 340
XapI RAATTY 2 cut(s) 613, 1345
XceI RCATGY 1 cut(s) 1002
XhoI CTCGAG 2 cut(s) 293, 822
XmiI GTMKAC 1 cut(s) 213
XspI CTAG 2 cut(s) 608, 815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.