RchiOBHm_Chr7g0218911

O-methyltransferase that catalyzes the 2 O-methylation steps in the ubiquinone biosynthetic pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0003217
Physical Location & Seq
Forward (+)
37119941 .. 37126199
6259 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 663 bp
ATGAATCCTACAAGGCTTGCCTTCATTCGCTCAACGCTGTGCCGTCATTTCAGGAGGGACCCGTTATCTGCTAGGCCCTTTGAAGGGCTCAGATTTATTGATGTTGGCTGTGGAGGTGGGATTCTTTCTGAGCCTTTAGCTCGGATGGGAGCTATTGTAACAGGAGTTGATGCTGTTGAGAAAAACATCAAGATAGCTCGCCTTCACGCAGACTTGGATCCAGTGACCTTAAAAATTGAATATCAATGCACTACAGCTGAAAAGCTAGTCGAGGAGCAAAAGAGTTTTGATGCTGTGTTTGCTTTAGAGGTGATTGAGCATGTGGAAAATCCTGCTGATTTCTGTAAATCTCTGTCAGCCTTAACTGTTCCCAGTGGAGCTACTGTGATTTCAACCATTAACCGTTCTATGAGAGCATATGCCACTGCCATAATTGCAGCCGAGTATCTGCTCCACTGGCTTCCTAAAGGTACACATCAATGGTCAACTTTTCTTACTCCTGAAGAACTAATCCTGATCCTGGAGCGTGCTTCTATTTCAGTCCAAGAGATGGCTGGGTTTGCATACAACCCTTTGACAGGAAGATGGTCAATATCTGATGATGTTAGTGTAAATTTCATCGCTTATGGTACTAAAACCAAAGCAGGCAATTTAGAAATATAA

Protein Analysis

220

Amino Acids

24.27

Weight (kDa)

5.97

Isoelectric Point (pI)

37.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_23 PF13489 18 - 177 1.3e-19 Methyltransferase domain
Methyltransf_31 PF13847 28 - 163 1.4e-13 Methyltransferase domain
CMAS PF02353 28 - 140 4.8e-06 Mycolic acid cyclopropane synthetase
PrmA PF06325 31 - 133 2.2e-06 Ribosomal protein L11 methyltransferase (PrmA)
Methyltransf_11 PF08241 33 - 129 2.1e-19 Methyltransferase domain
Methyltransf_25 PF13649 33 - 126 6.9e-15 Methyltransferase domain
Methyltransf_12 PF08242 33 - 126 5.8e-11 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 550
AclWI GGATC 3 cut(s) 212, 225, 511
AcsI RAATTY 1 cut(s) 613
AcuI CTGAAG 1 cut(s) 522
AfaI GTAC 2 cut(s) 472, 631
AfiI CCNNNNNNNGG 5 cut(s) 83, 84, 520, 550, 578
AgsI TTSAA 3 cut(s) 83, 239, 393
AjnI CCWGG 1 cut(s) 519
AluBI AGCT 6 cut(s) 140, 152, 197, 257, 265, 380
AluI AGCT 6 cut(s) 140, 152, 197, 257, 265, 380
AlwI GGATC 3 cut(s) 212, 225, 511
AoxI GGCC 1 cut(s) 74
ApeKI GCWGC 1 cut(s) 437
ApoI RAATTY 1 cut(s) 613
AspS9I GGNCC 2 cut(s) 58, 75
AsuHPI GGTGA 1 cut(s) 322
AvaII GGWCC 1 cut(s) 58
BamHI GGATCC 1 cut(s) 217
BanII GRGCYC 1 cut(s) 90
BbvI GCAGC 1 cut(s) 449
BccI CCATC 3 cut(s) 139, 544, 579
BceAI ACGGC 1 cut(s) 27
BciT130I CCWGG 1 cut(s) 521
BfaI CTAG 2 cut(s) 72, 266
BfmI CTRYAG 1 cut(s) 252
BisI GCNGC 1 cut(s) 438
BlsI GCNGC 1 cut(s) 439
Bme1390I CCNGG 1 cut(s) 521
Bme18I GGWCC 1 cut(s) 58
BmgT120I GGNCC 2 cut(s) 58, 75
BmiI GGNNCC 3 cut(s) 59, 60, 219
BmrFI CCNGG 1 cut(s) 521
BmrI ACTGGG 1 cut(s) 366
BmsI GCATC 2 cut(s) 160, 280
BmuI ACTGGG 1 cut(s) 366
BpmI CTGGAG 1 cut(s) 542
BsaXI ACNNNNNCTCC 4 cut(s) 141, 171, 369, 399
Bsc4I CCNNNNNNNGG 5 cut(s) 83, 84, 520, 550, 578
Bse1I ACTGG 3 cut(s) 221, 372, 461
BseBI CCWGG 1 cut(s) 521
BseGI GGATG 1 cut(s) 150
BseLI CCNNNNNNNGG 5 cut(s) 83, 84, 520, 550, 578
BseMII CTCAG 2 cut(s) 103, 120
BseNI ACTGG 3 cut(s) 221, 372, 461
BseRI GAGGAG 1 cut(s) 287
BseXI GCAGC 1 cut(s) 449
BseYI CCCAGC 1 cut(s) 554
BshFI GGCC 1 cut(s) 76
BslFI GGGAC 1 cut(s) 71
BslI CCNNNNNNNGG 5 cut(s) 83, 84, 520, 550, 578
BsmFI GGGAC 1 cut(s) 71
BsnI GGCC 1 cut(s) 76
Bsp1286I GDGCHC 1 cut(s) 90
Bsp143I GATC 2 cut(s) 217, 516
BspANI GGCC 1 cut(s) 76
BspCNI CTCAG 2 cut(s) 102, 121
BspLI GGNNCC 3 cut(s) 59, 60, 219
BspPI GGATC 3 cut(s) 212, 225, 511
BsrI ACTGG 3 cut(s) 221, 372, 461
BssMI GATC 2 cut(s) 217, 516
Bst2UI CCWGG 1 cut(s) 521
Bst4CI ACNGT 3 cut(s) 367, 385, 404
BstC8I GCNNGC 4 cut(s) 18, 199, 528, 646
BstDEI CTNAG 2 cut(s) 89, 129
BstENI CCTNNNNNAGG 1 cut(s) 576
BstF5I GGATG 1 cut(s) 150
BstKTI GATC 2 cut(s) 220, 519
BstMBI GATC 2 cut(s) 217, 516
BstMWI GCNNNNNNNGC 4 cut(s) 299, 434, 457, 560
BstNI CCWGG 1 cut(s) 521
BstNSI RCATGY 1 cut(s) 323
BstSCI CCNGG 1 cut(s) 519
BstSFI CTRYAG 1 cut(s) 252
BstV1I GCAGC 1 cut(s) 449
BstX2I RGATCY 1 cut(s) 217
BstYI RGATCY 1 cut(s) 217
BsuRI GGCC 1 cut(s) 76
BtgZI GCGATG 1 cut(s) 604
BtsCI GGATG 1 cut(s) 150
BtsI GCAGTG 1 cut(s) 423
BtsIMutI CAGTG 4 cut(s) 228, 379, 423, 454
Cac8I GCNNGC 4 cut(s) 18, 199, 528, 646
Cfr13I GGNCC 2 cut(s) 58, 75
Csp6I GTAC 2 cut(s) 471, 630
CviAII CATG 1 cut(s) 320
CviQI GTAC 2 cut(s) 471, 630
DdeI CTNAG 2 cut(s) 89, 129
DpnI GATC 2 cut(s) 219, 518
DpnII GATC 2 cut(s) 217, 516
Eco24I GRGCYC 1 cut(s) 90
Eco47I GGWCC 1 cut(s) 58
Eco57I CTGAAG 1 cut(s) 522
EcoNI CCTNNNNNAGG 1 cut(s) 576
EcoO109I RGGNCCY 2 cut(s) 58, 75
EcoRII CCWGG 1 cut(s) 519
EcoT38I GRGCYC 1 cut(s) 90
FaeI CATG 1 cut(s) 323
FaiI YATR 8 cut(s) 321, 410, 418, 420, 431, 565, 627, 661
FaqI GGGAC 1 cut(s) 71
FatI CATG 1 cut(s) 319
FauNDI CATATG 1 cut(s) 418
Fnu4HI GCNGC 1 cut(s) 438
FokI GGATG 1 cut(s) 157
FriOI GRGCYC 1 cut(s) 90
Fsp4HI GCNGC 1 cut(s) 438
FspBI CTAG 2 cut(s) 72, 266
GluI GCNGC 1 cut(s) 438
GsaI CCCAGC 1 cut(s) 558
GsuI CTGGAG 1 cut(s) 542
HaeIII GGCC 1 cut(s) 76
Hin1II CATG 1 cut(s) 323
HincII GTYRAC 1 cut(s) 486
HindII GTYRAC 1 cut(s) 486
HinfI GANTC 2 cut(s) 4, 121
HphI GGTGA 1 cut(s) 322
Hpy166II GTNNAC 2 cut(s) 473, 486
Hpy188I TCNGA 4 cut(s) 92, 130, 144, 598
Hpy188III TCNNGA 4 cut(s) 52, 190, 500, 514
Hpy8I GTNNAC 2 cut(s) 473, 486
HpyAV CCTTC 3 cut(s) 31, 77, 212
HpyCH4III ACNGT 3 cut(s) 367, 385, 404
HpyCH4V TGCA 3 cut(s) 249, 437, 563
HpyF10VI GCNNNNNNNGC 4 cut(s) 299, 434, 457, 560
HpyF3I CTNAG 2 cut(s) 89, 129
Hsp92II CATG 1 cut(s) 323
KflI GGGWCCC 1 cut(s) 58
Kzo9I GATC 2 cut(s) 217, 516
LmnI GCTCC 5 cut(s) 149, 274, 377, 456, 523
Lsp1109I GCAGC 1 cut(s) 449
LweI GCATC 2 cut(s) 160, 280
MaeI CTAG 2 cut(s) 72, 266
MaeIII GTNAC 2 cut(s) 157, 223
MalI GATC 2 cut(s) 219, 518
MboI GATC 2 cut(s) 217, 516
MboII GAAGA 2 cut(s) 515, 594
MflI RGATCY 1 cut(s) 217
MhlI GDGCHC 1 cut(s) 90
MluCI AATT 4 cut(s) 234, 432, 613, 649
MnlI CCTC 4 cut(s) 48, 107, 265, 301
MseI TTAA 3 cut(s) 230, 362, 399
MslI CAYNNNNRTG 1 cut(s) 478
MspA1I CMGCKG 1 cut(s) 257
MspR9I CCNGG 1 cut(s) 521
MvaI CCWGG 1 cut(s) 521
MwoI GCNNNNNNNGC 4 cut(s) 299, 434, 457, 560
NdeI CATATG 1 cut(s) 418
NdeII GATC 2 cut(s) 217, 516
NlaIII CATG 1 cut(s) 323
NlaIV GGNNCC 3 cut(s) 59, 60, 219
NmeAIII GCCGAG 1 cut(s) 466
NmuCI GTSAC 1 cut(s) 223
NspI RCATGY 1 cut(s) 323
PfeI GAWTC 2 cut(s) 4, 121
PflMI CCANNNNNTGG 1 cut(s) 550
PfoI TCCNGGA 1 cut(s) 519
PkrI GCNGC 1 cut(s) 439
PpuMI RGGWCCY 1 cut(s) 58
Psp5II RGGWCCY 1 cut(s) 58
Psp6I CCWGG 1 cut(s) 519
PspFI CCCAGC 1 cut(s) 554
PspGI CCWGG 1 cut(s) 519
PspN4I GGNNCC 3 cut(s) 59, 60, 219
PspPI GGNCC 2 cut(s) 58, 75
PspPPI RGGWCCY 1 cut(s) 58
PsuI RGATCY 1 cut(s) 217
PvuII CAGCTG 1 cut(s) 257
RsaI GTAC 2 cut(s) 472, 631
RsaNI GTAC 2 cut(s) 471, 630
RseI CAYNNNNRTG 1 cut(s) 478
SaqAI TTAA 3 cut(s) 230, 362, 399
SatI GCNGC 1 cut(s) 438
Sau3AI GATC 2 cut(s) 217, 516
Sau96I GGNCC 2 cut(s) 58, 75
ScrFI CCNGG 1 cut(s) 521
SduI GDGCHC 1 cut(s) 90
SfaNI GCATC 2 cut(s) 160, 280
SfcI CTRYAG 1 cut(s) 252
SinI GGWCC 1 cut(s) 58
SmiMI CAYNNNNRTG 1 cut(s) 478
Sse9I AATT 4 cut(s) 234, 432, 613, 649
SspMI CTAG 2 cut(s) 72, 266
StyD4I CCNGG 1 cut(s) 519
TaaI ACNGT 3 cut(s) 367, 385, 404
TaqI TCGA 1 cut(s) 270
TasI AATT 4 cut(s) 234, 432, 613, 649
TfiI GAWTC 2 cut(s) 4, 121
Tru1I TTAA 3 cut(s) 230, 362, 399
Tru9I TTAA 3 cut(s) 230, 362, 399
TscAI CASTG 4 cut(s) 228, 379, 430, 461
TseFI GTSAC 1 cut(s) 223
TseI GCWGC 1 cut(s) 437
Tsp45I GTSAC 1 cut(s) 223
TspDTI ATGAA 3 cut(s) 13, 17, 607
TspRI CASTG 4 cut(s) 228, 379, 430, 461
Van91I CCANNNNNTGG 1 cut(s) 550
VpaK11BI GGWCC 1 cut(s) 58
XagI CCTNNNNNAGG 1 cut(s) 576
XapI RAATTY 1 cut(s) 613
XceI RCATGY 1 cut(s) 323
XcmI CCANNNNNNNNNTGG 1 cut(s) 551
XspI CTAG 2 cut(s) 72, 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.