RchiOBHm_Chr5g0072231

Endo-1,4-beta-xylanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002751
Physical Location & Seq
Forward (+)
78194829 .. 78195768
940 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 888 bp
ATGCTCAAGGGTGGCCTAACTGCGAATGCATCAAGCGCAGCTGAGCTTTATTTTGAGAGTAAGAACACATCGGTTGAGATTTGGGTTGATAGCGTCTCATTACAACCATTCAACAAAAAGGAATGGAAGTCTCATTATGATCAAAGCATTGACAAGAACCGAAAGAGCAACGTGAGAATCCAAGCAGTTGATGCAAAAGGAAACCCTCTACGGAATGCAACAATATCCATCCAACAAAAGGCTCCCAGTTTCCCTTTCGGATGCGCTATGAACAAGAACATTCTCACCAATACTGCTTACCAAAACTGGTTCACTTCGAGGTTTGGAGTCACAACTTTTGAAGACGAAATGAAATGGTACAGCACAGAACCTTCACAGGGACAAGAGGACTACTCAGTGGCTGATGCCCTGCTTCAGTTTGCTAAACAACAGGACATTGCAGTCAGAGGCCACAATGTGTTCTGGGATGATCCTCAGTACCATCTAGGTTGGGTCAAATCACTCGATAAACAACAACTGGCTCTGGCTGCTTCCAAGAGGTTAAATTCTTTGGGTGAAAATGCTTCAGCCATTTTATACAATTGGGCTACTAAAGCTGATGGAGCAACCACCTTGTTCATGAATGACTACAATACCATTGAGGAGAGCAAAGATGGAGCCTCCACCCCGGCTAAGTACCTTCAAAAGCTCAGAGATATACAAGGCTTTCCCGGTAACAACAACTTAAGGATGGCAATAGGACTTGAGTCTCATTTCAAGACTCCTAACATTCCTTACATAAGATCTCCAATTGATACTCTGGCTGCGCTGCAAACCTACCAATCTGGATTACAAAGTTGGATGTGGAAAGCGGTCCCAATCAAGCATGGTACTTGGAGCAGATTCTGA

Protein Analysis

295

Amino Acids

33.31

Weight (kDa)

8.92

Isoelectric Point (pI)

48.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_10 PF00331 83 - 171 3.6e-17 Glycosyl hydrolase family 10
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 440
AciI CCGC 1 cut(s) 851
AclWI GGATC 1 cut(s) 464
AcsI RAATTY 1 cut(s) 544
AcuI CTGAAG 2 cut(s) 398, 549
AdeI CACNNNGTG 1 cut(s) 457
AfaI GTAC 4 cut(s) 359, 479, 677, 871
AfiI CCNNNNNNNGG 2 cut(s) 238, 377
AflII CTTAAG 1 cut(s) 724
AgsI TTSAA 4 cut(s) 112, 341, 683, 757
AluBI AGCT 4 cut(s) 41, 46, 596, 688
AluI AGCT 4 cut(s) 41, 46, 596, 688
Alw26I GTCTC 3 cut(s) 100, 135, 753
AlwI GGATC 1 cut(s) 464
AlwNI CAGNNNCTG 2 cut(s) 401, 885
AoxI GGCC 2 cut(s) 13, 448
ApeKI GCWGC 4 cut(s) 38, 527, 803, 808
ApoI RAATTY 1 cut(s) 544
AspLEI GCGC 3 cut(s) 38, 266, 808
AspS9I GGNCC 1 cut(s) 853
AsuC2I CCSGG 2 cut(s) 668, 711
AsuHPI GGTGA 2 cut(s) 277, 566
AvaII GGWCC 1 cut(s) 853
BbsI GAAGAC 1 cut(s) 348
BbvI GCAGC 4 cut(s) 50, 514, 790, 795
BccI CCATC 5 cut(s) 236, 489, 593, 647, 724
BclI TGATCA 1 cut(s) 139
BcnI CCSGG 2 cut(s) 668, 711
BcoDI GTCTC 3 cut(s) 100, 135, 753
BfaI CTAG 1 cut(s) 485
BfrI CTTAAG 1 cut(s) 724
BglII AGATCT 1 cut(s) 782
BisI GCNGC 4 cut(s) 39, 528, 804, 809
BlpI GCTNAGC 1 cut(s) 42
BlsI GCNGC 4 cut(s) 40, 529, 805, 810
Bme1390I CCNGG 2 cut(s) 668, 711
Bme18I GGWCC 1 cut(s) 853
BmgT120I GGNCC 1 cut(s) 853
BmiI GGNNCC 3 cut(s) 243, 658, 855
BmrFI CCNGG 2 cut(s) 668, 711
BmrI ACTGGG 1 cut(s) 240
BmsI GCATC 4 cut(s) 38, 181, 251, 394
BmuI ACTGGG 1 cut(s) 240
BoxI GACNNNNGTC 1 cut(s) 745
BpiI GAAGAC 1 cut(s) 348
BplI GAGNNNNNCTC 2 cut(s) 377, 409
Bpu1102I GCTNAGC 1 cut(s) 42
BpuEI CTTGAG 1 cut(s) 764
BpuMI CCSGG 2 cut(s) 668, 711
BsaJI CCNNGG 1 cut(s) 666
Bsc4I CCNNNNNNNGG 2 cut(s) 238, 377
Bse1I ACTGG 3 cut(s) 246, 311, 522
Bse3DI GCAATG 1 cut(s) 435
BseDI CCNNGG 1 cut(s) 666
BseGI GGATG 5 cut(s) 228, 266, 472, 735, 846
BseLI CCNNNNNNNGG 2 cut(s) 238, 377
BseMI GCAATG 1 cut(s) 435
BseMII CTCAG 4 cut(s) 33, 408, 488, 703
BseNI ACTGG 3 cut(s) 246, 311, 522
BseRI GAGGAG 1 cut(s) 656
BseXI GCAGC 4 cut(s) 50, 514, 790, 795
BshFI GGCC 2 cut(s) 15, 450
BsiSI CCGG 2 cut(s) 668, 711
BslFI GGGAC 2 cut(s) 393, 839
BslI CCNNNNNNNGG 2 cut(s) 238, 377
BsmAI GTCTC 3 cut(s) 100, 135, 753
BsmBI CGTCTC 1 cut(s) 100
BsmFI GGGAC 2 cut(s) 393, 839
BsmI GAATGC 2 cut(s) 31, 220
BsnI GGCC 2 cut(s) 15, 450
Bsp143I GATC 3 cut(s) 139, 469, 782
Bsp1720I GCTNAGC 1 cut(s) 42
BspACI CCGC 1 cut(s) 851
BspANI GGCC 2 cut(s) 15, 450
BspCNI CTCAG 4 cut(s) 34, 407, 487, 702
BspHI TCATGA 1 cut(s) 618
BspLI GGNNCC 3 cut(s) 243, 658, 855
BspPI GGATC 1 cut(s) 464
BspTI CTTAAG 1 cut(s) 724
BsrDI GCAATG 1 cut(s) 435
BsrI ACTGG 3 cut(s) 246, 311, 522
BssECI CCNNGG 1 cut(s) 666
BssMI GATC 3 cut(s) 139, 469, 782
BstAFI CTTAAG 1 cut(s) 724
BstAPI GCANNNNNTGC 1 cut(s) 191
BstDEI CTNAG 5 cut(s) 42, 394, 474, 672, 689
BstF5I GGATG 5 cut(s) 228, 266, 472, 735, 846
BstHHI GCGC 3 cut(s) 38, 266, 808
BstKTI GATC 3 cut(s) 142, 472, 785
BstMAI GTCTC 3 cut(s) 100, 135, 753
BstMBI GATC 3 cut(s) 139, 469, 782
BstMWI GCNNNNNNNGC 5 cut(s) 35, 191, 527, 593, 602
BstPAI GACNNNNGTC 1 cut(s) 745
BstSCI CCNGG 2 cut(s) 666, 709
BstV1I GCAGC 4 cut(s) 50, 514, 790, 795
BstV2I GAAGAC 1 cut(s) 348
BstX2I RGATCY 1 cut(s) 782
BstYI RGATCY 1 cut(s) 782
BsuRI GGCC 2 cut(s) 15, 450
BtsCI GGATG 5 cut(s) 228, 266, 472, 735, 846
BtsIMutI CAGTG 1 cut(s) 402
CaiI CAGNNNCTG 2 cut(s) 401, 885
CciI TCATGA 1 cut(s) 618
CfoI GCGC 3 cut(s) 38, 266, 808
Cfr13I GGNCC 1 cut(s) 853
CseI GACGC 1 cut(s) 82
Csp6I GTAC 4 cut(s) 358, 478, 676, 870
CviAII CATG 2 cut(s) 619, 866
CviQI GTAC 4 cut(s) 358, 478, 676, 870
DdeI CTNAG 5 cut(s) 42, 394, 474, 672, 689
DpnI GATC 3 cut(s) 141, 471, 784
DpnII GATC 3 cut(s) 139, 469, 782
DraIII CACNNNGTG 1 cut(s) 457
DrdI GACNNNNNNGTC 1 cut(s) 440
DseDI GACNNNNNNGTC 1 cut(s) 440
Eco47I GGWCC 1 cut(s) 853
Eco57I CTGAAG 2 cut(s) 398, 549
EcoT22I ATGCAT 1 cut(s) 31
Esp3I CGTCTC 1 cut(s) 100
FaeI CATG 2 cut(s) 622, 869
FaiI YATR 7 cut(s) 138, 269, 577, 620, 698, 779, 867
FaqI GGGAC 2 cut(s) 393, 839
FatI CATG 2 cut(s) 618, 865
FbaI TGATCA 1 cut(s) 139
Fnu4HI GCNGC 4 cut(s) 39, 528, 804, 809
FokI GGATG 5 cut(s) 215, 273, 479, 742, 853
Fsp4HI GCNGC 4 cut(s) 39, 528, 804, 809
FspBI CTAG 1 cut(s) 485
GlaI GCGC 3 cut(s) 37, 265, 807
GluI GCNGC 4 cut(s) 39, 528, 804, 809
HaeIII GGCC 2 cut(s) 15, 450
HapII CCGG 2 cut(s) 668, 711
HgaI GACGC 1 cut(s) 82
HhaI GCGC 3 cut(s) 38, 266, 808
Hin1II CATG 2 cut(s) 622, 869
Hin6I GCGC 3 cut(s) 36, 264, 806
HinP1I GCGC 3 cut(s) 36, 264, 806
HinfI GANTC 5 cut(s) 177, 327, 746, 760, 882
HpaII CCGG 2 cut(s) 668, 711
HphI GGTGA 2 cut(s) 277, 566
Hpy166II GTNNAC 1 cut(s) 312
Hpy188I TCNGA 4 cut(s) 260, 446, 692, 887
Hpy188III TCNNGA 3 cut(s) 619, 757, 825
Hpy8I GTNNAC 1 cut(s) 312
HpyAV CCTTC 2 cut(s) 381, 689
HpyCH4IV ACGT 1 cut(s) 171
HpyCH4V TGCA 5 cut(s) 29, 194, 218, 440, 811
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 191, 527, 593, 602
HpyF3I CTNAG 5 cut(s) 42, 394, 474, 672, 689
HpySE526I ACGT 1 cut(s) 171
Hsp92II CATG 2 cut(s) 622, 869
HspAI GCGC 3 cut(s) 36, 264, 806
Ksp22I TGATCA 1 cut(s) 139
Kzo9I GATC 3 cut(s) 139, 469, 782
LmnI GCTCC 4 cut(s) 247, 602, 656, 876
Lsp1109I GCAGC 4 cut(s) 50, 514, 790, 795
LweI GCATC 4 cut(s) 38, 181, 251, 394
MaeI CTAG 1 cut(s) 485
MaeII ACGT 1 cut(s) 171
MaeIII GTNAC 2 cut(s) 328, 713
MalI GATC 3 cut(s) 141, 471, 784
MboI GATC 3 cut(s) 139, 469, 782
MboII GAAGA 1 cut(s) 353
MfeI CAATTG 2 cut(s) 580, 789
MflI RGATCY 1 cut(s) 782
MluCI AATT 3 cut(s) 544, 580, 789
MlyI GAGTC 3 cut(s) 336, 754, 755
MmeI TCCRAC 2 cut(s) 256, 818
MnlI CCTC 8 cut(s) 216, 312, 379, 440, 483, 531, 634, 670
Mph1103I ATGCAT 1 cut(s) 31
MseI TTAA 2 cut(s) 542, 725
MspA1I CMGCKG 1 cut(s) 41
MspCI CTTAAG 1 cut(s) 724
MspI CCGG 2 cut(s) 668, 711
MspR9I CCNGG 2 cut(s) 668, 711
MunI CAATTG 2 cut(s) 580, 789
Mva1269I GAATGC 2 cut(s) 31, 220
MwoI GCNNNNNNNGC 5 cut(s) 35, 191, 527, 593, 602
NciI CCSGG 2 cut(s) 668, 711
NdeII GATC 3 cut(s) 139, 469, 782
NlaIII CATG 2 cut(s) 622, 869
NlaIV GGNNCC 3 cut(s) 243, 658, 855
NmuCI GTSAC 1 cut(s) 328
NsiI ATGCAT 1 cut(s) 31
PagI TCATGA 1 cut(s) 618
PctI GAATGC 2 cut(s) 31, 220
PfeI GAWTC 2 cut(s) 177, 882
PkrI GCNGC 4 cut(s) 40, 529, 805, 810
PleI GAGTC 3 cut(s) 335, 754, 754
PpsI GAGTC 3 cut(s) 335, 754, 754
PshAI GACNNNNGTC 1 cut(s) 745
PspN4I GGNNCC 3 cut(s) 243, 658, 855
PspPI GGNCC 1 cut(s) 853
PstNI CAGNNNCTG 2 cut(s) 401, 885
PsuI RGATCY 1 cut(s) 782
PvuII CAGCTG 1 cut(s) 41
RsaI GTAC 4 cut(s) 359, 479, 677, 871
RsaNI GTAC 4 cut(s) 358, 478, 676, 870
SaqAI TTAA 2 cut(s) 542, 725
SatI GCNGC 4 cut(s) 39, 528, 804, 809
Sau3AI GATC 3 cut(s) 139, 469, 782
Sau96I GGNCC 1 cut(s) 853
SchI GAGTC 3 cut(s) 336, 754, 755
ScrFI CCNGG 2 cut(s) 668, 711
SfaNI GCATC 4 cut(s) 38, 181, 251, 394
SinI GGWCC 1 cut(s) 853
SmlI CTYRAG 3 cut(s) 5, 724, 743
SmoI CTYRAG 3 cut(s) 5, 724, 743
Sse9I AATT 3 cut(s) 544, 580, 789
SsiI CCGC 1 cut(s) 851
SspMI CTAG 1 cut(s) 485
StyD4I CCNGG 2 cut(s) 666, 709
TaiI ACGT 1 cut(s) 174
TaqI TCGA 2 cut(s) 317, 504
TasI AATT 3 cut(s) 544, 580, 789
TfiI GAWTC 2 cut(s) 177, 882
Tru1I TTAA 2 cut(s) 542, 725
Tru9I TTAA 2 cut(s) 542, 725
TscAI CASTG 1 cut(s) 402
TseFI GTSAC 1 cut(s) 328
TseI GCWGC 4 cut(s) 38, 527, 803, 808
Tsp45I GTSAC 1 cut(s) 328
TspDTI ATGAA 4 cut(s) 284, 365, 607, 635
TspGWI ACGGA 1 cut(s) 226
TspRI CASTG 1 cut(s) 402
Vha464I CTTAAG 1 cut(s) 724
VpaK11BI GGWCC 1 cut(s) 853
XapI RAATTY 1 cut(s) 544
XspI CTAG 1 cut(s) 485
Zsp2I ATGCAT 1 cut(s) 31
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.