RchiOBHm_Chr3g0465291

Aldo-keto reductase family 4 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0002704
Physical Location & Seq
Reverse (-)
12115071 .. 12117220
2150 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 969 bp
ATGGCGAACGCAATTCGGTTTTTTGAGTTGAACACCGGCGCCAAGATGCCTTCTTTAGGCTTAGGCACTTATCGCTCCGACACTCATCTCGTGGGCGACGTCCTCACCTCTGCAATTAAGATTGGATATCGTCATGTTGACTGTGCCCAAATGTATGGCAATGAGAAGGAGATTGGTGATGCTTTGAAGAAGCTATGTAATGATGGGATAGTGAAGCGTGAGGAATTATGGGTCACTTCCAAACTCTGGTGTACTGATCATGAACCAGAAGATGTACCGAAGGCATTTGATAAAACTTTGCAAGATTTGCAACTTGACTATCTGGATCTTTATCTTATGCATTGGCCAGTCAGCATGAAAAAGGGGTCAGTTGGCTCTGATCCTGAAAAATTTACCACACCTAACATACCCAGTACATGGAAAGCAATGGAGGCACTGTATGATTCAGGCAAGGCCAGGGCAATTGGAGTCAGCAATTTCTCATCAAAGAAGCTTGGAGAACTGTTAGAGGTGGCACGAATACATCCAGCAGTTAATCAAGTTGAGTTGCACCCTCAATGGCAGCAAGCTAAGCTGCATTCTTTCTGTAAATCTAAGGGAATCCACTTAACTGGATATGCACCATTGGGAAGGCAAAAGGTGCTTGAACATTCTATTGTGAATATGGTGGCAGAAAAATTGGGCAAGACTCCTGCACAAGTAGTTCTTCGGTGGGGGATGCAAATGGGTCACAGTGTAGTGCCTAAAAGCACAAATCAGATGAGGATTCAGGAAAATTTTAATATTTTTGATTGGTCTATACCTGATAACTTGTTTGCCAAGTTTTCTGAAATTGAGCAGGCAAGTGCCTATTCCTTTATCCCCTTCTTTTCTTTTCTTCACACTGAGAGGCTGCTTAAAGGTACTGCATTCGTCCACAAGACTCACGGTTTTTACAAGACCATTGAGGAACTCTGGGATGGAGAGTAG

Protein Analysis

322

Amino Acids

36.49

Weight (kDa)

7.22

Isoelectric Point (pI)

36.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 21 - 278 3.7e-48 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 102
AccB1I GGYRCC 1 cut(s) 38
AccB7I CCANNNNNTGG 2 cut(s) 246, 417
AclWI GGATC 2 cut(s) 333, 374
AcoI YGGCCR 1 cut(s) 344
AcsI RAATTY 2 cut(s) 389, 775
AcyI GRCGYC 2 cut(s) 39, 99
AfaI GTAC 4 cut(s) 253, 276, 415, 904
AfiI CCNNNNNNNGG 3 cut(s) 56, 246, 417
AgsI TTSAA 3 cut(s) 31, 187, 647
AjnI CCWGG 1 cut(s) 455
AluBI AGCT 4 cut(s) 193, 493, 569, 574
AluI AGCT 4 cut(s) 193, 493, 569, 574
AlwI GGATC 2 cut(s) 333, 374
AoxI GGCC 2 cut(s) 344, 453
ApeKI GCWGC 3 cut(s) 562, 574, 892
ApoI RAATTY 2 cut(s) 389, 775
AspLEI GCGC 1 cut(s) 41
AsuHPI GGTGA 2 cut(s) 97, 188
BaeGI GKGCMC 1 cut(s) 148
BalI TGGCCA 1 cut(s) 346
BanI GGYRCC 1 cut(s) 38
BauI CACGAG 1 cut(s) 89
BbvI GCAGC 3 cut(s) 561, 574, 879
BccI CCATC 2 cut(s) 197, 953
BciT130I CCWGG 1 cut(s) 457
BclI TGATCA 1 cut(s) 256
BfoI RGCGCY 1 cut(s) 42
BisI GCNGC 3 cut(s) 563, 575, 893
BlpI GCTNAGC 1 cut(s) 570
BlsI GCNGC 3 cut(s) 564, 576, 894
Bme1390I CCNGG 1 cut(s) 457
BmiI GGNNCC 1 cut(s) 40
BmrFI CCNGG 1 cut(s) 457
BmrI ACTGGG 1 cut(s) 405
BmsI GCATC 3 cut(s) 36, 169, 708
BmuI ACTGGG 1 cut(s) 405
Bpu10I CCTNAGC 1 cut(s) 61
Bpu1102I GCTNAGC 1 cut(s) 570
BsaBI GATNNNNATC 1 cut(s) 330
BsaHI GRCGYC 2 cut(s) 39, 99
BsaJI CCNNGG 1 cut(s) 456
BsaXI ACNNNNNCTCC 2 cut(s) 422, 452
Bsc4I CCNNNNNNNGG 3 cut(s) 56, 246, 417
Bse118I RCCGGY 1 cut(s) 35
Bse1I ACTGG 3 cut(s) 347, 411, 616
Bse3DI GCAATG 2 cut(s) 166, 432
Bse8I GATNNNNATC 1 cut(s) 330
BseBI CCWGG 1 cut(s) 457
BseDI CCNNGG 1 cut(s) 456
BseGI GGATG 3 cut(s) 523, 723, 964
BseJI GATNNNNATC 1 cut(s) 330
BseLI CCNNNNNNNGG 3 cut(s) 56, 246, 417
BseMI GCAATG 2 cut(s) 166, 432
BseMII CTCAG 1 cut(s) 876
BseNI ACTGG 3 cut(s) 347, 411, 616
BseSI GKGCMC 1 cut(s) 148
BseXI GCAGC 3 cut(s) 561, 574, 879
BsgI GTGCAG 1 cut(s) 678
BshFI GGCC 2 cut(s) 346, 455
BshNI GGYRCC 1 cut(s) 38
BsiSI CCGG 1 cut(s) 36
BslI CCNNNNNNNGG 3 cut(s) 56, 246, 417
BsmI GAATGC 2 cut(s) 577, 908
BsnI GGCC 2 cut(s) 346, 455
Bsp1286I GDGCHC 1 cut(s) 148
Bsp143I GATC 3 cut(s) 256, 325, 379
Bsp1720I GCTNAGC 1 cut(s) 570
BspANI GGCC 2 cut(s) 346, 455
BspCNI CTCAG 1 cut(s) 877
BspHI TCATGA 1 cut(s) 259
BspLI GGNNCC 1 cut(s) 40
BspPI GGATC 2 cut(s) 333, 374
BspT107I GGYRCC 1 cut(s) 38
BsrDI GCAATG 2 cut(s) 166, 432
BsrFI RCCGGY 1 cut(s) 35
BsrI ACTGG 3 cut(s) 347, 411, 616
BssAI RCCGGY 1 cut(s) 35
BssECI CCNNGG 1 cut(s) 456
BssMI GATC 3 cut(s) 256, 325, 379
BssNI GRCGYC 2 cut(s) 39, 99
BssSI CACGAG 1 cut(s) 89
Bst2BI CACGAG 1 cut(s) 89
Bst2UI CCWGG 1 cut(s) 457
Bst4CI ACNGT 5 cut(s) 143, 438, 504, 734, 929
BstACI GRCGYC 2 cut(s) 39, 99
BstAPI GCANNNNNTGC 2 cut(s) 307, 640
BstC8I GCNNGC 2 cut(s) 567, 840
BstDEI CTNAG 4 cut(s) 61, 570, 594, 885
BstENI CCTNNNNNAGG 1 cut(s) 54
BstF5I GGATG 3 cut(s) 523, 723, 964
BstH2I RGCGCY 1 cut(s) 42
BstHHI GCGC 1 cut(s) 41
BstKTI GATC 3 cut(s) 259, 328, 382
BstMBI GATC 3 cut(s) 256, 325, 379
BstMWI GCNNNNNNNGC 5 cut(s) 72, 307, 431, 571, 640
BstNI CCWGG 1 cut(s) 457
BstSCI CCNGG 1 cut(s) 455
BstSLI GKGCMC 1 cut(s) 148
BstV1I GCAGC 3 cut(s) 561, 574, 879
BstX2I RGATCY 1 cut(s) 325
BstXI CCANNNNNNTGG 2 cut(s) 155, 611
BstYI RGATCY 1 cut(s) 325
BsuRI GGCC 2 cut(s) 346, 455
BtsCI GGATG 3 cut(s) 523, 723, 964
BtsIMutI CAGTG 3 cut(s) 434, 739, 882
Cac8I GCNNGC 2 cut(s) 567, 840
CciI TCATGA 1 cut(s) 259
CfoI GCGC 1 cut(s) 41
Cfr10I RCCGGY 1 cut(s) 35
Csp6I GTAC 4 cut(s) 252, 275, 414, 903
CviAII CATG 4 cut(s) 134, 260, 355, 417
CviJI RGCY 9 cut(s) 60, 193, 346, 375, 455, 493, 569, 574, 892
CviKI_1 RGCY 9 cut(s) 60, 193, 346, 375, 455, 493, 569, 574, 892
CviQI GTAC 4 cut(s) 252, 275, 414, 903
DdeI CTNAG 4 cut(s) 61, 570, 594, 885
DinI GGCGCC 1 cut(s) 40
DpnI GATC 3 cut(s) 258, 327, 381
DpnII GATC 3 cut(s) 256, 325, 379
EaeI YGGCCR 1 cut(s) 344
Eco32I GATATC 1 cut(s) 128
EcoNI CCTNNNNNAGG 1 cut(s) 54
EcoRII CCWGG 1 cut(s) 455
EcoRV GATATC 1 cut(s) 128
EcoT22I ATGCAT 1 cut(s) 342
EgeI GGCGCC 1 cut(s) 40
EheI GGCGCC 1 cut(s) 40
FaeI CATG 4 cut(s) 137, 263, 358, 420
FalI AAGNNNNNCTT 2 cut(s) 690, 722
FatI CATG 4 cut(s) 133, 259, 354, 416
FbaI TGATCA 1 cut(s) 256
Fnu4HI GCNGC 3 cut(s) 563, 575, 893
FokI GGATG 2 cut(s) 510, 730
Fsp4HI GCNGC 3 cut(s) 563, 575, 893
GlaI GCGC 1 cut(s) 40
GluI GCNGC 3 cut(s) 563, 575, 893
HaeII RGCGCY 1 cut(s) 42
HaeIII GGCC 2 cut(s) 346, 455
HapII CCGG 1 cut(s) 36
HhaI GCGC 1 cut(s) 41
Hin1I GRCGYC 2 cut(s) 39, 99
Hin1II CATG 4 cut(s) 137, 263, 358, 420
Hin6I GCGC 1 cut(s) 39
HinP1I GCGC 1 cut(s) 39
HincII GTYRAC 1 cut(s) 139
HindII GTYRAC 1 cut(s) 139
HindIII AAGCTT 1 cut(s) 491
HinfI GANTC 6 cut(s) 443, 468, 600, 688, 766, 922
HpaII CCGG 1 cut(s) 36
HphI GGTGA 2 cut(s) 97, 188
Hpy166II GTNNAC 3 cut(s) 139, 252, 916
Hpy188I TCNGA 4 cut(s) 79, 379, 759, 829
Hpy188III TCNNGA 4 cut(s) 260, 323, 383, 770
Hpy8I GTNNAC 3 cut(s) 139, 252, 916
Hpy99I CGWCG 1 cut(s) 101
HpyAV CCTTC 5 cut(s) 60, 160, 274, 624, 874
HpyCH4III ACNGT 5 cut(s) 143, 438, 504, 734, 929
HpyCH4IV ACGT 1 cut(s) 99
HpyF10VI GCNNNNNNNGC 5 cut(s) 72, 307, 431, 571, 640
HpyF3I CTNAG 4 cut(s) 61, 570, 594, 885
HpySE526I ACGT 1 cut(s) 99
Hsp92I GRCGYC 2 cut(s) 39, 99
Hsp92II CATG 4 cut(s) 137, 263, 358, 420
HspAI GCGC 1 cut(s) 39
KasI GGCGCC 1 cut(s) 38
Ksp22I TGATCA 1 cut(s) 256
Kzo9I GATC 3 cut(s) 256, 325, 379
LmnI GCTCC 1 cut(s) 80
Lsp1109I GCAGC 3 cut(s) 561, 574, 879
LweI GCATC 3 cut(s) 36, 169, 708
MaeII ACGT 1 cut(s) 99
MaeIII GTNAC 2 cut(s) 232, 728
MalI GATC 3 cut(s) 258, 327, 381
MboI GATC 3 cut(s) 256, 325, 379
MboII GAAGA 4 cut(s) 199, 281, 698, 869
MfeI CAATTG 1 cut(s) 462
MflI RGATCY 1 cut(s) 325
MhlI GDGCHC 1 cut(s) 148
MlsI TGGCCA 1 cut(s) 346
MluCI AATT 9 cut(s) 12, 114, 224, 389, 462, 475, 677, 775, 831
MluNI TGGCCA 1 cut(s) 346
Mly113I GGCGCC 1 cut(s) 39
MlyI GAGTC 3 cut(s) 477, 682, 916
MmeI TCCRAC 1 cut(s) 102
MnlI CCTC 9 cut(s) 113, 118, 214, 424, 502, 564, 756, 882, 940
Mox20I TGGCCA 1 cut(s) 346
Mph1103I ATGCAT 1 cut(s) 342
MscI TGGCCA 1 cut(s) 346
MseI TTAA 5 cut(s) 117, 534, 608, 780, 897
Msp20I TGGCCA 1 cut(s) 346
MspI CCGG 1 cut(s) 36
MspR9I CCNGG 1 cut(s) 457
MunI CAATTG 1 cut(s) 462
Mva1269I GAATGC 2 cut(s) 577, 908
MvaI CCWGG 1 cut(s) 457
MwoI GCNNNNNNNGC 5 cut(s) 72, 307, 431, 571, 640
NarI GGCGCC 1 cut(s) 39
NdeII GATC 3 cut(s) 256, 325, 379
NlaIII CATG 4 cut(s) 137, 263, 358, 420
NlaIV GGNNCC 1 cut(s) 40
NmuCI GTSAC 2 cut(s) 232, 728
NsiI ATGCAT 1 cut(s) 342
PagI TCATGA 1 cut(s) 259
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PctI GAATGC 2 cut(s) 577, 908
PfeI GAWTC 3 cut(s) 443, 600, 766
PflMI CCANNNNNTGG 2 cut(s) 246, 417
PkrI GCNGC 3 cut(s) 564, 576, 894
PleI GAGTC 3 cut(s) 476, 682, 916
PluTI GGCGCC 1 cut(s) 42
PpsI GAGTC 3 cut(s) 476, 682, 916
Psp6I CCWGG 1 cut(s) 455
PspGI CCWGG 1 cut(s) 455
PspN4I GGNNCC 1 cut(s) 40
PsuI RGATCY 1 cut(s) 325
RsaI GTAC 4 cut(s) 253, 276, 415, 904
RsaNI GTAC 4 cut(s) 252, 275, 414, 903
SaqAI TTAA 5 cut(s) 117, 534, 608, 780, 897
SatI GCNGC 3 cut(s) 563, 575, 893
Sau3AI GATC 3 cut(s) 256, 325, 379
SchI GAGTC 3 cut(s) 477, 682, 916
ScrFI CCNGG 1 cut(s) 457
SduI GDGCHC 1 cut(s) 148
SfaNI GCATC 3 cut(s) 36, 169, 708
SfoI GGCGCC 1 cut(s) 40
SgrAI CRCCGGYG 1 cut(s) 35
Sse9I AATT 9 cut(s) 12, 114, 224, 389, 462, 475, 677, 775, 831
SspDI GGCGCC 1 cut(s) 38
SspI AATATT 1 cut(s) 784
StyD4I CCNGG 1 cut(s) 455
TaaI ACNGT 5 cut(s) 143, 438, 504, 734, 929
TaiI ACGT 1 cut(s) 102
TasI AATT 9 cut(s) 12, 114, 224, 389, 462, 475, 677, 775, 831
TatI WGTACW 2 cut(s) 251, 413
TfiI GAWTC 3 cut(s) 443, 600, 766
Tru1I TTAA 5 cut(s) 117, 534, 608, 780, 897
Tru9I TTAA 5 cut(s) 117, 534, 608, 780, 897
TscAI CASTG 3 cut(s) 441, 739, 889
TseFI GTSAC 2 cut(s) 232, 728
TseI GCWGC 3 cut(s) 562, 574, 892
Tsp45I GTSAC 2 cut(s) 232, 728
TspDTI ATGAA 2 cut(s) 276, 371
TspRI CASTG 3 cut(s) 441, 739, 889
Van91I CCANNNNNTGG 2 cut(s) 246, 417
XagI CCTNNNNNAGG 1 cut(s) 54
XapI RAATTY 2 cut(s) 389, 775
ZraI GACGTC 1 cut(s) 100
Zsp2I ATGCAT 1 cut(s) 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.