RchiOBHm_Chr2g0142431

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000481
Physical Location & Seq
Forward (+)
59918838 .. 59921680
2843 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1077 bp
ATGGCTGCAGTTGCTCAGGCCTTAGAGACCCCCTCATCTGTTACCAGCATCAAATCACTAGCCGAGTCCTCTGCTCTAAACTCTGTCCCTTCTGCCTATGCCTTCAACAGAAACCCCAATGAGGAAGCAGATCCAAACGATCCCGAATTCGCAATTCCAATCATCGATATGTCACTCCTCACATCTGGTACTCCTGAACAACGCTCCCAAATCATCCATGACCTCGTCAAAATTTGTCAAGAATGGGGCTTCTTCATAGCAATTAACCACGGAGTGCCAGAGAGCCTAATGAAGGCCATGATTGACGCCTGTCACGGCTTCTTCAGTCTTCCAGACGAGGAGAAAAAGGAATTCAAATCAGGGAATGATGTGCTAGAAATGTTCAAGTATGGTACCAGCTATAACCTGGCCTTGGATAAGGTCCTTCTCTGGAGGGACTTCTTCAAGGTCAGAACACATCCTGAGTTTTACTCCCTCTACAAACCGGCTACCTTCAGTGAGGTTTCAATGGAGTTTGGCAAAAGAACAAGAGAAGTGGCACTAGAAATCATAAAAGCAATATCTGAAAGCCTGGGATTGGAGCCGAATTACATATACGAAAAGATGAACATGGACCGTGGCTTACAATTGCTGGCCGGGAACTTCTATCCTCCTTGCCCTCAGCCGGAACAGGCCATTGGTATACCACATCACACCGATCACGGTCTAGTCACACTCCTCATCCAGAATGAGATGACCGGTCTCCAAGTCGAACACAACGGAAAATGGCTCACCGTTAACGGCCCTGCCAATGGGCTTTTTGTTAACCTTGCTGATCAAATGCAGATTCTTACCAACGGTAAATACAAGAGTGTGATGCACAGGGCGACTGTGAACAACAAAGCAACGAGGATATCAATAGCTATACCACATGGACCTTCTGTAGATACAACTATTGCTCCAGCTCCAGAGCTGTGTGAAAAAGATGGCAAACCTCCCAAGTACCTTGCCATGAACTACAAGGAGTACATTCAGCTTCAGCAGAGCGGCAAGAACTACATGAAGACCGGATCCGCATTCGATCAAATACTAGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

358

Amino Acids

39.91

Weight (kDa)

5.62

Isoelectric Point (pI)

36.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 52 - 156 6.1e-26 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 209 - 303 1.7e-27 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000481)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g20420 FvH4_5g20421 FvH4_5g20430 FvH4_5g20431 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g33280
malus_domestica MD03G1140300.v1.1 MD03G1140400.v1.1 MD11G1171500.v1.1
prunus_persica Prupe.8G112300_v2.0.a1
pyrus_communis pycom03g09520 pycom11g13270
rosa_chinensis RchiOBHm_Chr2g0142431 RchiOBHm_Chr5g0068661 RchiOBHm_Chr6g0271401 RchiOBHm_Chr7g0205561 RchiOBHm_Chr7g0205581 RchiOBHm_Chr7g0205721 RchiOBHm_Chr7g0205741
rosa_laevigata RLG00000003357 RLG00000003358 RLG00000003359 RLG00000003363 RLG00000009087 RLG00000019958 RLG00000035989
rosa_multiflora Rmu_sc0000076.1_g000053 Rmu_sc0000076.1_g000054 Rmu_sc0000076.1_g000055 Rmu_sc0001211.1_g000022 Rmu_sc0001679.1_g000017 Rmu_sc0008518.1_g000018 Rmu_sc0008518.1_g000022 Rmu_sc0008518.1_g000024 Rmu_ssc0000482.1_g000004
rosa_roxburghii Rroxscaffold_1G00012230 Rroxscaffold_2G00102620 Rroxscaffold_3G00252010 Rroxscaffold_3G00252020 Rroxscaffold_3G00252030 Rroxscaffold_3G00252050 Rroxscaffold_7G00197030
rosa_rugosa Rorug02G0372500 Rorug02G0372600 Rorug06G0060800 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092700 Rorug07G0092800
rosa_samantha Rh2AG424300 Rh2BG433900 Rh2CG410900 Rh2DG443900 Rh5AG449900 Rh5DG481500 Rh5DG481700 Rh6AG180000 Rh6BG183200 Rh6CG179900 Rh6DG171700 Rh7AG222800 Rh7AG223900 Rh7AG224200 Rh7AG224300 Rh7BG219100 Rh7BG219200 Rh7BG219300 Rh7BG219500 Rh7CG235600 Rh7CG237400 Rh7CG237700 Rh7CG237800 Rh7DG229400 Rh7DG229600 Rh7DG231000
rosa_wichuraiana Rw2G034750 Rw5G042000 Rw6G015460 Rw7G019220 Rw7G019310 Rw7G019320 Rw7G019330 Rw7G019340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 392
AccB1I GGYRCC 1 cut(s) 392
AccBSI CCGCTC 1 cut(s) 1026
AccI GTMKAC 1 cut(s) 682
AciI CCGC 2 cut(s) 1026, 1053
AclWI GGATC 4 cut(s) 125, 134, 1044, 1057
AcoI YGGCCR 1 cut(s) 633
AcsI RAATTY 3 cut(s) 146, 231, 350
AcuI CTGAAG 3 cut(s) 307, 478, 1001
AcyI GRCGYC 1 cut(s) 306
AdeI CACNNNGTG 1 cut(s) 274
AfaI GTAC 4 cut(s) 190, 394, 983, 1007
AfiI CCNNNNNNNGG 6 cut(s) 121, 292, 412, 577, 664, 791
AgeI ACCGGT 1 cut(s) 737
AgsI TTSAA 5 cut(s) 106, 355, 385, 445, 507
AjnI CCWGG 2 cut(s) 405, 570
AjuI GAANNNNNNNTTGG 2 cut(s) 660, 692
AluBI AGCT 5 cut(s) 399, 902, 944, 952, 1015
AluI AGCT 5 cut(s) 399, 902, 944, 952, 1015
Alw26I GTCTC 2 cut(s) 20, 746
AlwI GGATC 4 cut(s) 125, 134, 1044, 1057
AoxI GGCC 6 cut(s) 18, 294, 408, 633, 672, 781
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 3 cut(s) 146, 231, 350
AsiGI ACCGGT 1 cut(s) 737
Asp718I GGTACC 1 cut(s) 392
AspS9I GGNCC 4 cut(s) 421, 613, 782, 914
AsuC2I CCSGG 1 cut(s) 637
AsuHPI GGTGA 1 cut(s) 763
AvaII GGWCC 3 cut(s) 421, 613, 914
BamHI GGATCC 1 cut(s) 1049
BanI GGYRCC 1 cut(s) 392
BbsI GAAGAC 2 cut(s) 320, 1049
BbvCI CCTCAGC 1 cut(s) 660
BccI CCATC 1 cut(s) 959
BceAI ACGGC 2 cut(s) 331, 796
BcgI CGANNNNNNTGC 2 cut(s) 53, 87
BciT130I CCWGG 2 cut(s) 407, 572
BclI TGATCA 1 cut(s) 814
BcnI CCSGG 1 cut(s) 637
BcoDI GTCTC 2 cut(s) 20, 746
BfaI CTAG 6 cut(s) 59, 374, 542, 707, 1070, 1075
BfmI CTRYAG 2 cut(s) 6, 921
BisI GCNGC 2 cut(s) 6, 1027
BlsI GCNGC 2 cut(s) 7, 1028
Bme1390I CCNGG 3 cut(s) 407, 572, 637
Bme18I GGWCC 3 cut(s) 421, 613, 914
BmgT120I GGNCC 4 cut(s) 421, 613, 782, 914
BmiI GGNNCC 3 cut(s) 394, 582, 1051
BmrFI CCNGG 3 cut(s) 407, 572, 637
BmsI GCATC 2 cut(s) 57, 846
BoxI GACNNNNGTC 1 cut(s) 309
BpiI GAAGAC 2 cut(s) 320, 1049
BplI GAGNNNNNCTC 4 cut(s) 17, 49, 455, 487
BpmI CTGGAG 3 cut(s) 451, 924, 930
Bpu10I CCTNAGC 2 cut(s) 15, 660
BpuMI CCSGG 1 cut(s) 637
Bsa29I ATCGAT 1 cut(s) 165
BsaHI GRCGYC 1 cut(s) 306
BsaI GGTCTC 2 cut(s) 20, 746
BsaJI CCNNGG 4 cut(s) 268, 411, 571, 616
BsaWI WCCGGW 2 cut(s) 737, 1046
BsaXI ACNNNNNCTCC 2 cut(s) 922, 952
Bsc4I CCNNNNNNNGG 6 cut(s) 121, 292, 412, 577, 664, 791
Bse118I RCCGGY 2 cut(s) 484, 737
BseBI CCWGG 2 cut(s) 407, 572
BseCI ATCGAT 1 cut(s) 165
BseDI CCNNGG 4 cut(s) 268, 411, 571, 616
BseGI GGATG 3 cut(s) 213, 457, 720
BseLI CCNNNNNNNGG 6 cut(s) 121, 292, 412, 577, 664, 791
BseMII CTCAG 3 cut(s) 29, 453, 674
BseRI GAGGAG 3 cut(s) 167, 353, 707
BshFI GGCC 6 cut(s) 20, 296, 410, 635, 674, 783
BshNI GGYRCC 1 cut(s) 392
BshTI ACCGGT 1 cut(s) 737
BshVI ATCGAT 1 cut(s) 165
BsiSI CCGG 5 cut(s) 485, 636, 665, 738, 1047
BslFI GGGAC 2 cut(s) 71, 449
BslI CCNNNNNNNGG 6 cut(s) 121, 292, 412, 577, 664, 791
BsmAI GTCTC 2 cut(s) 20, 746
BsmFI GGGAC 2 cut(s) 71, 449
BsmI GAATGC 1 cut(s) 1055
BsnI GGCC 6 cut(s) 20, 296, 410, 635, 674, 783
Bso31I GGTCTC 2 cut(s) 20, 746
Bsp143I GATC 6 cut(s) 130, 139, 697, 814, 1049, 1060
BspACI CCGC 2 cut(s) 1026, 1053
BspANI GGCC 6 cut(s) 20, 296, 410, 635, 674, 783
BspCNI CTCAG 3 cut(s) 28, 454, 673
BspDI ATCGAT 1 cut(s) 165
BspLI GGNNCC 3 cut(s) 394, 582, 1051
BspMAI CTGCAG 1 cut(s) 10
BspPI GGATC 4 cut(s) 125, 134, 1044, 1057
BspT107I GGYRCC 1 cut(s) 392
BspTNI GGTCTC 2 cut(s) 20, 746
BsrBI CCGCTC 1 cut(s) 1026
BsrFI RCCGGY 2 cut(s) 484, 737
BssAI RCCGGY 2 cut(s) 484, 737
BssECI CCNNGG 4 cut(s) 268, 411, 571, 616
BssMI GATC 6 cut(s) 130, 139, 697, 814, 1049, 1060
BssNAI GTATAC 1 cut(s) 683
BssNI GRCGYC 1 cut(s) 306
BssT1I CCWWGG 1 cut(s) 411
Bst1107I GTATAC 1 cut(s) 683
Bst2UI CCWGG 2 cut(s) 407, 572
Bst4CI ACNGT 5 cut(s) 617, 704, 775, 839, 871
BstACI GRCGYC 1 cut(s) 306
BstC8I GCNNGC 1 cut(s) 633
BstDEI CTNAG 4 cut(s) 15, 22, 462, 660
BstDSI CCRYGG 2 cut(s) 268, 616
BstENI CCTNNNNNAGG 1 cut(s) 290
BstF5I GGATG 3 cut(s) 213, 457, 720
BstKTI GATC 6 cut(s) 133, 142, 700, 817, 1052, 1063
BstMAI GTCTC 2 cut(s) 20, 746
BstMBI GATC 6 cut(s) 130, 139, 697, 814, 1049, 1060
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 2 cut(s) 407, 572
BstPAI GACNNNNGTC 1 cut(s) 309
BstSCI CCNGG 3 cut(s) 405, 570, 635
BstSFI CTRYAG 2 cut(s) 6, 921
BstV2I GAAGAC 2 cut(s) 320, 1049
BstX2I RGATCY 2 cut(s) 130, 1049
BstYI RGATCY 2 cut(s) 130, 1049
BstZ17I GTATAC 1 cut(s) 683
Bsu15I ATCGAT 1 cut(s) 165
BsuRI GGCC 6 cut(s) 20, 296, 410, 635, 674, 783
BsuTUI ATCGAT 1 cut(s) 165
BtgI CCRYGG 2 cut(s) 268, 616
BtsCI GGATG 3 cut(s) 213, 457, 720
BtsIMutI CAGTG 1 cut(s) 502
Cac8I GCNNGC 1 cut(s) 633
Cfr10I RCCGGY 2 cut(s) 484, 737
Cfr13I GGNCC 4 cut(s) 421, 613, 782, 914
ClaI ATCGAT 1 cut(s) 165
CseI GACGC 1 cut(s) 314
Csp6I GTAC 4 cut(s) 189, 393, 982, 1006
CspAI ACCGGT 1 cut(s) 737
CspCI CAANNNNNGTGG 2 cut(s) 516, 551
CviAII CATG 6 cut(s) 218, 298, 610, 911, 991, 1039
CviQI GTAC 4 cut(s) 189, 393, 982, 1006
DdeI CTNAG 4 cut(s) 15, 22, 462, 660
DpnI GATC 6 cut(s) 132, 141, 699, 816, 1051, 1062
DpnII GATC 6 cut(s) 130, 139, 697, 814, 1049, 1060
DraIII CACNNNGTG 1 cut(s) 274
EaeI YGGCCR 1 cut(s) 633
Eco130I CCWWGG 1 cut(s) 411
Eco147I AGGCCT 1 cut(s) 20
Eco31I GGTCTC 2 cut(s) 20, 746
Eco32I GATATC 1 cut(s) 894
Eco47I GGWCC 3 cut(s) 421, 613, 914
Eco57I CTGAAG 3 cut(s) 307, 478, 1001
EcoNI CCTNNNNNAGG 1 cut(s) 290
EcoO109I RGGNCCY 1 cut(s) 421
EcoRI GAATTC 2 cut(s) 146, 350
EcoRII CCWGG 2 cut(s) 405, 570
EcoRV GATATC 1 cut(s) 894
EcoT14I CCWWGG 1 cut(s) 411
ErhI CCWWGG 1 cut(s) 411
FaeI CATG 6 cut(s) 221, 301, 613, 914, 994, 1042
FaqI GGGAC 2 cut(s) 71, 449
FatI CATG 6 cut(s) 217, 297, 609, 910, 990, 1038
FbaI TGATCA 1 cut(s) 814
FblI GTMKAC 1 cut(s) 682
Fnu4HI GCNGC 2 cut(s) 6, 1027
FokI GGATG 3 cut(s) 200, 444, 707
Fsp4HI GCNGC 2 cut(s) 6, 1027
FspBI CTAG 6 cut(s) 59, 374, 542, 707, 1070, 1075
GluI GCNGC 2 cut(s) 6, 1027
GsuI CTGGAG 3 cut(s) 451, 924, 930
HaeIII GGCC 6 cut(s) 20, 296, 410, 635, 674, 783
HapII CCGG 5 cut(s) 485, 636, 665, 738, 1047
HgaI GACGC 1 cut(s) 314
Hin1I GRCGYC 1 cut(s) 306
Hin1II CATG 6 cut(s) 221, 301, 613, 914, 994, 1042
HincII GTYRAC 2 cut(s) 778, 805
HindII GTYRAC 2 cut(s) 778, 805
HinfI GANTC 2 cut(s) 65, 826
HpaI GTTAAC 2 cut(s) 778, 805
HpaII CCGG 5 cut(s) 485, 636, 665, 738, 1047
HphI GGTGA 1 cut(s) 763
Hpy166II GTNNAC 4 cut(s) 683, 778, 805, 874
Hpy188I TCNGA 2 cut(s) 452, 565
Hpy188III TCNNGA 8 cut(s) 143, 194, 239, 332, 430, 461, 724, 947
Hpy8I GTNNAC 4 cut(s) 683, 778, 805, 874
HpyAV CCTTC 6 cut(s) 99, 112, 286, 434, 502, 927
HpyCH4III ACNGT 5 cut(s) 617, 704, 775, 839, 871
HpyCH4V TGCA 3 cut(s) 8, 823, 859
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 4 cut(s) 15, 22, 462, 660
Hsp92I GRCGYC 1 cut(s) 306
Hsp92II CATG 6 cut(s) 221, 301, 613, 914, 994, 1042
KpnI GGTACC 1 cut(s) 396
Ksp22I TGATCA 1 cut(s) 814
KspAI GTTAAC 2 cut(s) 778, 805
Kzo9I GATC 6 cut(s) 130, 139, 697, 814, 1049, 1060
LmnI GCTCC 4 cut(s) 209, 580, 943, 949
LweI GCATC 2 cut(s) 57, 846
MaeI CTAG 6 cut(s) 59, 374, 542, 707, 1070, 1075
MaeIII GTNAC 4 cut(s) 40, 171, 311, 709
MalI GATC 6 cut(s) 132, 141, 699, 816, 1051, 1062
MbiI CCGCTC 1 cut(s) 1026
MboI GATC 6 cut(s) 130, 139, 697, 814, 1049, 1060
MboII GAAGA 5 cut(s) 244, 313, 320, 433, 1054
MfeI CAATTG 1 cut(s) 626
MflI RGATCY 2 cut(s) 130, 1049
MluCI AATT 7 cut(s) 146, 153, 231, 261, 350, 586, 626
MlyI GAGTC 1 cut(s) 74
MseI TTAA 3 cut(s) 264, 777, 804
MslI CAYNNNNRTG 1 cut(s) 167
MspI CCGG 5 cut(s) 485, 636, 665, 738, 1047
MspR9I CCNGG 3 cut(s) 407, 572, 637
MunI CAATTG 1 cut(s) 626
Mva1269I GAATGC 1 cut(s) 1055
MvaI CCWGG 2 cut(s) 407, 572
MwoI GCNNNNNNNGC 1 cut(s) 11
NciI CCSGG 1 cut(s) 637
NdeII GATC 6 cut(s) 130, 139, 697, 814, 1049, 1060
NlaIII CATG 6 cut(s) 221, 301, 613, 914, 994, 1042
NlaIV GGNNCC 3 cut(s) 394, 582, 1051
NmeAIII GCCGAG 1 cut(s) 88
NmuCI GTSAC 3 cut(s) 171, 311, 709
PceI AGGCCT 1 cut(s) 20
PctI GAATGC 1 cut(s) 1055
PfeI GAWTC 1 cut(s) 826
PflFI GACNNNGTC 1 cut(s) 224
PinAI ACCGGT 1 cut(s) 737
PkrI GCNGC 2 cut(s) 7, 1028
PleI GAGTC 1 cut(s) 73
PpsI GAGTC 1 cut(s) 73
PpuMI RGGWCCY 1 cut(s) 421
PshAI GACNNNNGTC 1 cut(s) 309
Psp5II RGGWCCY 1 cut(s) 421
Psp6I CCWGG 2 cut(s) 405, 570
PspGI CCWGG 2 cut(s) 405, 570
PspN4I GGNNCC 3 cut(s) 394, 582, 1051
PspPI GGNCC 4 cut(s) 421, 613, 782, 914
PspPPI RGGWCCY 1 cut(s) 421
PstI CTGCAG 1 cut(s) 10
PsuI RGATCY 2 cut(s) 130, 1049
PsyI GACNNNGTC 1 cut(s) 224
RsaI GTAC 4 cut(s) 190, 394, 983, 1007
RsaNI GTAC 4 cut(s) 189, 393, 982, 1006
RseI CAYNNNNRTG 1 cut(s) 167
SaqAI TTAA 3 cut(s) 264, 777, 804
SatI GCNGC 2 cut(s) 6, 1027
Sau3AI GATC 6 cut(s) 130, 139, 697, 814, 1049, 1060
Sau96I GGNCC 4 cut(s) 421, 613, 782, 914
SchI GAGTC 1 cut(s) 74
ScrFI CCNGG 3 cut(s) 407, 572, 637
SfaNI GCATC 2 cut(s) 57, 846
SfcI CTRYAG 2 cut(s) 6, 921
SinI GGWCC 3 cut(s) 421, 613, 914
SmiMI CAYNNNNRTG 1 cut(s) 167
Sse9I AATT 7 cut(s) 146, 153, 231, 261, 350, 586, 626
SseBI AGGCCT 1 cut(s) 20
SsiI CCGC 2 cut(s) 1026, 1053
SspMI CTAG 6 cut(s) 59, 374, 542, 707, 1070, 1075
StuI AGGCCT 1 cut(s) 20
StyD4I CCNGG 3 cut(s) 405, 570, 635
StyI CCWWGG 1 cut(s) 411
TaaI ACNGT 5 cut(s) 617, 704, 775, 839, 871
TaqI TCGA 3 cut(s) 165, 750, 1059
TasI AATT 7 cut(s) 146, 153, 231, 261, 350, 586, 626
TatI WGTACW 1 cut(s) 1005
TauI GCSGC 1 cut(s) 1029
TfiI GAWTC 1 cut(s) 826
Tru1I TTAA 3 cut(s) 264, 777, 804
Tru9I TTAA 3 cut(s) 264, 777, 804
TscAI CASTG 1 cut(s) 502
TseFI GTSAC 3 cut(s) 171, 311, 709
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 3 cut(s) 171, 311, 709
TspDTI ATGAA 5 cut(s) 244, 305, 620, 1007, 1055
TspGWI ACGGA 2 cut(s) 285, 774
TspRI CASTG 1 cut(s) 502
Tth111I GACNNNGTC 1 cut(s) 224
VpaK11BI GGWCC 3 cut(s) 421, 613, 914
XagI CCTNNNNNAGG 1 cut(s) 290
XapI RAATTY 3 cut(s) 146, 231, 350
XcmI CCANNNNNNNNNTGG 1 cut(s) 403
XmiI GTMKAC 1 cut(s) 682
XspI CTAG 6 cut(s) 59, 374, 542, 707, 1070, 1075
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.