RchiOBHm_Chr1g0375091

phosphopyruvate hydratase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0002577
Physical Location & Seq
Reverse (-)
62663909 .. 62667243
3335 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1341 bp
ATGGCGACCACGATCAAGCTCATCAAGGCCCGTCAGATCTTCGACAGCCGTGGAAATCCCACCGTTGAAGTTGATGTTACTCTTTCCGATGGAACTCTGGCCAGAGCGGCCGTGCCGAGTGGAGCTTCCACAGGTGTATATGAAGCACTGGAGTTGAGAGATGGCGGATCGGATTACCTTGGGAAGGGTGTCCTGAAGGCTGTGGAGAATGTGAATGGTATCATTGGACCTGCCTTGATTGGAAAGGACCCAACAGAACAGACCCAAATTGACAATTATATGGTTCAACAGCTTGATGGAACTACAAATGAATGGGGTTGGTGCAAACAGAAGCTTGGAGCAAATGCAATATTGGCAGTGTCTCTTGCTGTTTGTAAAGCAGGTGCCATTGTGAAAAAGATACCCCTATACAAGCACATAGCCAATCTTGCTGGAAACAAGACCTTGGTTCTGCCTGTTCCTGCATTTAATGTCATCAATGGAGGTTCTCATGCTGGCAATAAACTAGCCATGCAGGAATTTATGATTCTCCCTGTTGGAGCATCTTCTTTTAAGGAAGCCATGAAGATGGGTGTAGAAGTATATCATCATCTGAAGGCTGTAATTAAGAAGAAGTATGGACAAGATGCCACAAATGTTGGGGATGAAGGTGGCTTTGCACCCAATATTCAGGAAAACAAAGAGGGTCTTGAACTTCTGAAGACAGCTATTGCTAAAGCTGGATATACCGGAAAGGTTGTTATTGGGATGGATGTTGCTGCTTCAGAATTCTATGATGATAAGGATAAGACTTATGATTTGAATTTCAAGGAAGAGAAAAATGACGGATCACAAAGAATATCAGGAGACAGTCTGAAGAATGTTTACAAGTCATTTGTTACAGAGTATCCAATTGTGTCAATTGAAGATCCCTTTGATCAGGATGACTGGGAACACTATGCTAAGATGACTGCAGAAATTGGCGAGCAAGTGCAAATTGTTGGTGATGATCTCCTTGTTACAAACCCAAAGCGAGTGGAGAAAGCAATCAAGGAGAAGAGCTGCAATGCCCTTCTTTTGAAGGTGAATCAAATTGGTTCAGTAACCGAAAGTATTGAAGCTGTGAAAATGTCAAAACGTGCTGGATGGGGTGTCATGGCAAGTCACCGAAGTGGTGAAACTGAGGATACTTTCATTGCTGACCTTTCTGTGGGGCTTGCAACGGGCCAGATTAAGACTGGAGCTCCATGCAGATCCGAGCGTCTTGCTAAGTACAACCAGCTTCTTAGAATTGAAGAGGAGCTTGGGCCTGCAGCAGTCTATGCTGGATCAAAATTCAGAGTACCAGTGGAGCCATACTGA

Protein Analysis

446

Amino Acids

48.22

Weight (kDa)

5.85

Isoelectric Point (pI)

36.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Enolase_N PF03952 5 - 140 4e-53 Enolase, N-terminal domain
Enolase_C PF00113 149 - 440 1.2e-158 Enolase, C-terminal TIM barrel domain
MR_MLE_C PF13378 275 - 408 1.7e-08 Enolase C-terminal domain-like
MAAL_C PF07476 300 - 403 8.5e-06 Methylaspartate ammonia-lyase C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 371
Acc36I ACCTGC 2 cut(s) 238, 371
AccB1I GGYRCC 1 cut(s) 383
AccBSI CCGCTC 1 cut(s) 107
AciI CCGC 2 cut(s) 107, 165
AclWI GGATC 5 cut(s) 175, 835, 902, 1227, 1315
AcoI YGGCCR 2 cut(s) 99, 108
AcsI RAATTY 4 cut(s) 518, 767, 802, 1313
AcuI CTGAAG 5 cut(s) 215, 614, 719, 747, 875
AfaI GTAC 2 cut(s) 1253, 1323
AfiI CCNNNNNNNGG 2 cut(s) 184, 1189
AgsI TTSAA 9 cut(s) 68, 287, 692, 802, 808, 905, 1060, 1097, 1274
AjuI GAANNNNNNNTTGG 2 cut(s) 1266, 1298
AleI CACNNNNGTG 1 cut(s) 1149
Alw21I GWGCWC 1 cut(s) 1225
Alw26I GTCTC 2 cut(s) 366, 840
AlwI GGATC 5 cut(s) 175, 835, 902, 1227, 1315
AoxI GGCC 5 cut(s) 27, 99, 108, 1204, 1286
ApeKI GCWGC 3 cut(s) 758, 1041, 1292
ApoI RAATTY 4 cut(s) 518, 767, 802, 1313
ArsI GACNNNNNNTTYG 2 cut(s) 781, 813
AspS9I GGNCC 5 cut(s) 28, 227, 247, 1204, 1286
AsuHPI GGTGA 4 cut(s) 995, 1075, 1136, 1166
AvaII GGWCC 2 cut(s) 227, 247
BalI TGGCCA 1 cut(s) 101
BanI GGYRCC 1 cut(s) 383
BanII GRGCYC 1 cut(s) 1225
BarI GAAGNNNNNNTAC 2 cut(s) 848, 880
BbsI GAAGAC 1 cut(s) 707
Bbv12I GWGCWC 1 cut(s) 1225
BbvI GCAGC 3 cut(s) 745, 1028, 1304
BccI CCATC 6 cut(s) 83, 155, 290, 562, 742, 1119
BceAI ACGGC 2 cut(s) 33, 95
BcgI CGANNNNNNTGC 2 cut(s) 1226, 1260
BciVI GTATCC 2 cut(s) 897, 1159
BclI TGATCA 1 cut(s) 916
BcoDI GTCTC 2 cut(s) 366, 840
BfaI CTAG 1 cut(s) 506
BfmI CTRYAG 2 cut(s) 951, 1290
BfuAI ACCTGC 2 cut(s) 238, 371
BfuI GTATCC 2 cut(s) 897, 1159
BglI GCCNNNNNGGC 1 cut(s) 107
BglII AGATCT 1 cut(s) 36
BisI GCNGC 4 cut(s) 108, 759, 1042, 1293
BlsI GCNGC 4 cut(s) 109, 760, 1043, 1294
Bme18I GGWCC 2 cut(s) 227, 247
BmgT120I GGNCC 5 cut(s) 28, 227, 247, 1204, 1286
BmiI GGNNCC 3 cut(s) 249, 385, 1332
BmrI ACTGGG 1 cut(s) 937
BmsI GCATC 2 cut(s) 551, 616
BmuI ACTGGG 1 cut(s) 937
BpiI GAAGAC 1 cut(s) 707
BpmI CTGGAG 2 cut(s) 170, 1239
BsaJI CCNNGG 3 cut(s) 49, 178, 444
BsaWI WCCGGW 1 cut(s) 728
BsaXI ACNNNNNCTCC 2 cut(s) 1207, 1237
Bsc4I CCNNNNNNNGG 2 cut(s) 184, 1189
Bse1I ACTGG 4 cut(s) 153, 932, 1222, 1325
Bse3DI GCAATG 2 cut(s) 1051, 1173
BseDI CCNNGG 3 cut(s) 49, 178, 444
BseGI GGATG 5 cut(s) 649, 753, 757, 928, 1130
BseLI CCNNNNNNNGG 2 cut(s) 184, 1189
BseMI GCAATG 2 cut(s) 1051, 1173
BseMII CTCAG 1 cut(s) 1152
BseNI ACTGG 4 cut(s) 153, 932, 1222, 1325
BseRI GAGGAG 1 cut(s) 1292
BseX3I CGGCCG 1 cut(s) 108
BseXI GCAGC 3 cut(s) 745, 1028, 1304
Bsh1285I CGRYCG 1 cut(s) 111
BshFI GGCC 5 cut(s) 29, 101, 110, 1206, 1288
BshNI GGYRCC 1 cut(s) 383
BsiEI CGRYCG 1 cut(s) 111
BsiHKAI GWGCWC 1 cut(s) 1225
BsiSI CCGG 1 cut(s) 729
BslI CCNNNNNNNGG 2 cut(s) 184, 1189
BsmAI GTCTC 2 cut(s) 366, 840
BsnI GGCC 5 cut(s) 29, 101, 110, 1206, 1288
Bsp1286I GDGCHC 1 cut(s) 1225
Bsp143I GATC 9 cut(s) 12, 36, 167, 827, 907, 916, 988, 1232, 1307
BspACI CCGC 2 cut(s) 107, 165
BspANI GGCC 5 cut(s) 29, 101, 110, 1206, 1288
BspCNI CTCAG 1 cut(s) 1153
BspLI GGNNCC 3 cut(s) 249, 385, 1332
BspMAI CTGCAG 2 cut(s) 955, 1294
BspMI ACCTGC 2 cut(s) 238, 371
BspPI GGATC 5 cut(s) 175, 835, 902, 1227, 1315
BspQI GCTCTTC 1 cut(s) 1031
BspT107I GGYRCC 1 cut(s) 383
BsrBI CCGCTC 1 cut(s) 107
BsrDI GCAATG 2 cut(s) 1051, 1173
BsrI ACTGG 4 cut(s) 153, 932, 1222, 1325
BssECI CCNNGG 3 cut(s) 49, 178, 444
BssMI GATC 9 cut(s) 12, 36, 167, 827, 907, 916, 988, 1232, 1307
BssT1I CCWWGG 2 cut(s) 178, 444
Bst4CI ACNGT 2 cut(s) 64, 851
Bst6I CTCTTC 3 cut(s) 807, 1031, 1269
BstAPI GCANNNNNTGC 1 cut(s) 1301
BstC8I GCNNGC 4 cut(s) 496, 965, 1197, 1290
BstDEI CTNAG 4 cut(s) 942, 1161, 1248, 1265
BstDSI CCRYGG 1 cut(s) 49
BstENI CCTNNNNNAGG 1 cut(s) 182
BstF5I GGATG 5 cut(s) 649, 753, 757, 928, 1130
BstKTI GATC 9 cut(s) 15, 39, 170, 830, 910, 919, 991, 1235, 1310
BstMAI GTCTC 2 cut(s) 366, 840
BstMBI GATC 9 cut(s) 12, 36, 167, 827, 907, 916, 988, 1232, 1307
BstMCI CGRYCG 1 cut(s) 111
BstMWI GCNNNNNNNGC 4 cut(s) 107, 353, 428, 1301
BstSFI CTRYAG 2 cut(s) 951, 1290
BstV1I GCAGC 3 cut(s) 745, 1028, 1304
BstV2I GAAGAC 1 cut(s) 707
BstX2I RGATCY 3 cut(s) 36, 907, 1232
BstXI CCANNNNNNTGG 1 cut(s) 568
BstYI RGATCY 3 cut(s) 36, 907, 1232
BstZI CGGCCG 1 cut(s) 108
BsuI GTATCC 2 cut(s) 897, 1159
BsuRI GGCC 5 cut(s) 29, 101, 110, 1206, 1288
BtgI CCRYGG 1 cut(s) 49
BtsCI GGATG 5 cut(s) 649, 753, 757, 928, 1130
BtsI GCAGTG 1 cut(s) 363
BtsIMutI CAGTG 3 cut(s) 146, 363, 1332
BveI ACCTGC 2 cut(s) 238, 371
Cac8I GCNNGC 4 cut(s) 496, 965, 1197, 1290
Cfr13I GGNCC 5 cut(s) 28, 227, 247, 1204, 1286
CseI GACGC 1 cut(s) 1229
Csp6I GTAC 2 cut(s) 1252, 1322
CspCI CAANNNNNGTGG 4 cut(s) 619, 654, 996, 1031
CviAII CATG 5 cut(s) 491, 511, 562, 1135, 1227
CviQI GTAC 2 cut(s) 1252, 1322
DdeI CTNAG 4 cut(s) 942, 1161, 1248, 1265
DpnI GATC 9 cut(s) 14, 38, 169, 829, 909, 918, 990, 1234, 1309
DpnII GATC 9 cut(s) 12, 36, 167, 827, 907, 916, 988, 1232, 1307
EaeI YGGCCR 2 cut(s) 99, 108
EagI CGGCCG 1 cut(s) 108
Eam1104I CTCTTC 3 cut(s) 807, 1031, 1269
EarI CTCTTC 3 cut(s) 807, 1031, 1269
EciI GGCGGA 1 cut(s) 180
Ecl136II GAGCTC 1 cut(s) 1223
EclXI CGGCCG 1 cut(s) 108
Eco130I CCWWGG 2 cut(s) 178, 444
Eco24I GRGCYC 1 cut(s) 1225
Eco47I GGWCC 2 cut(s) 227, 247
Eco52I CGGCCG 1 cut(s) 108
Eco53kI GAGCTC 1 cut(s) 1223
Eco57I CTGAAG 5 cut(s) 215, 614, 719, 747, 875
EcoICRI GAGCTC 1 cut(s) 1223
EcoNI CCTNNNNNAGG 1 cut(s) 182
EcoO109I RGGNCCY 1 cut(s) 247
EcoRI GAATTC 1 cut(s) 767
EcoT14I CCWWGG 2 cut(s) 178, 444
EcoT38I GRGCYC 1 cut(s) 1225
ErhI CCWWGG 2 cut(s) 178, 444
FaeI CATG 5 cut(s) 494, 514, 565, 1138, 1230
FalI AAGNNNNNCTT 4 cut(s) 672, 704, 1266, 1298
FatI CATG 5 cut(s) 490, 510, 561, 1134, 1226
FbaI TGATCA 1 cut(s) 916
Fnu4HI GCNGC 4 cut(s) 108, 759, 1042, 1293
FokI GGATG 5 cut(s) 656, 760, 764, 935, 1137
FriOI GRGCYC 1 cut(s) 1225
Fsp4HI GCNGC 4 cut(s) 108, 759, 1042, 1293
FspBI CTAG 1 cut(s) 506
GluI GCNGC 4 cut(s) 108, 759, 1042, 1293
GsuI CTGGAG 2 cut(s) 170, 1239
HaeIII GGCC 5 cut(s) 29, 101, 110, 1206, 1288
HapII CCGG 1 cut(s) 729
HgaI GACGC 1 cut(s) 1229
Hin1II CATG 5 cut(s) 494, 514, 565, 1138, 1230
HindIII AAGCTT 1 cut(s) 332
HinfI GANTC 2 cut(s) 526, 1066
HpaII CCGG 1 cut(s) 729
HphI GGTGA 4 cut(s) 995, 1075, 1136, 1166
Hpy166II GTNNAC 1 cut(s) 865
Hpy188I TCNGA 9 cut(s) 36, 88, 172, 594, 699, 766, 855, 1237, 1319
Hpy188III TCNNGA 5 cut(s) 193, 671, 689, 843, 920
Hpy8I GTNNAC 1 cut(s) 865
HpyAV CCTTC 6 cut(s) 178, 190, 589, 641, 1054, 1061
HpyCH4III ACNGT 2 cut(s) 64, 851
HpyCH4IV ACGT 1 cut(s) 1117
HpyF10VI GCNNNNNNNGC 4 cut(s) 107, 353, 428, 1301
HpyF3I CTNAG 4 cut(s) 942, 1161, 1248, 1265
HpySE526I ACGT 1 cut(s) 1117
Hsp92II CATG 5 cut(s) 494, 514, 565, 1138, 1230
Ksp22I TGATCA 1 cut(s) 916
Kzo9I GATC 9 cut(s) 12, 36, 167, 827, 907, 916, 988, 1232, 1307
LguI GCTCTTC 1 cut(s) 1031
LmnI GCTCC 7 cut(s) 122, 338, 539, 1220, 1228, 1279, 1330
Lsp1109I GCAGC 3 cut(s) 745, 1028, 1304
LweI GCATC 2 cut(s) 551, 616
MaeI CTAG 1 cut(s) 506
MaeII ACGT 1 cut(s) 1117
MaeIII GTNAC 5 cut(s) 76, 877, 997, 1081, 1142
MalI GATC 9 cut(s) 14, 38, 169, 829, 909, 918, 990, 1234, 1309
MbiI CCGCTC 1 cut(s) 107
MboI GATC 9 cut(s) 12, 36, 167, 827, 907, 916, 988, 1232, 1307
MfeI CAATTG 2 cut(s) 891, 900
MflI RGATCY 3 cut(s) 36, 907, 1232
MhlI GDGCHC 1 cut(s) 1225
MlsI TGGCCA 1 cut(s) 101
MluNI TGGCCA 1 cut(s) 101
MmeI TCCRAC 1 cut(s) 517
MnlI CCTC 4 cut(s) 476, 676, 1156, 1270
Mox20I TGGCCA 1 cut(s) 101
MscI TGGCCA 1 cut(s) 101
MseI TTAA 4 cut(s) 468, 552, 606, 1212
MslI CAYNNNNRTG 2 cut(s) 566, 1149
Msp20I TGGCCA 1 cut(s) 101
MspI CCGG 1 cut(s) 729
MunI CAATTG 2 cut(s) 891, 900
MwoI GCNNNNNNNGC 4 cut(s) 107, 353, 428, 1301
NdeII GATC 9 cut(s) 12, 36, 167, 827, 907, 916, 988, 1232, 1307
NlaIII CATG 5 cut(s) 494, 514, 565, 1138, 1230
NlaIV GGNNCC 3 cut(s) 249, 385, 1332
NmeAIII GCCGAG 1 cut(s) 141
NmuCI GTSAC 1 cut(s) 1142
OliI CACNNNNGTG 1 cut(s) 1149
PaqCI CACCTGC 1 cut(s) 371
PciSI GCTCTTC 1 cut(s) 1031
PfeI GAWTC 2 cut(s) 526, 1066
PkrI GCNGC 4 cut(s) 109, 760, 1043, 1294
PpuMI RGGWCCY 1 cut(s) 247
Psp124BI GAGCTC 1 cut(s) 1225
Psp5II RGGWCCY 1 cut(s) 247
PspN4I GGNNCC 3 cut(s) 249, 385, 1332
PspPI GGNCC 5 cut(s) 28, 227, 247, 1204, 1286
PspPPI RGGWCCY 1 cut(s) 247
PstI CTGCAG 2 cut(s) 955, 1294
PsuI RGATCY 3 cut(s) 36, 907, 1232
RsaI GTAC 2 cut(s) 1253, 1323
RsaNI GTAC 2 cut(s) 1252, 1322
RseI CAYNNNNRTG 2 cut(s) 566, 1149
SacI GAGCTC 1 cut(s) 1225
SapI GCTCTTC 1 cut(s) 1031
SaqAI TTAA 4 cut(s) 468, 552, 606, 1212
SatI GCNGC 4 cut(s) 108, 759, 1042, 1293
Sau3AI GATC 9 cut(s) 12, 36, 167, 827, 907, 916, 988, 1232, 1307
Sau96I GGNCC 5 cut(s) 28, 227, 247, 1204, 1286
SduI GDGCHC 1 cut(s) 1225
SfaNI GCATC 2 cut(s) 551, 616
SfcI CTRYAG 2 cut(s) 951, 1290
SfiI GGCCNNNNNGGCC 1 cut(s) 107
SinI GGWCC 2 cut(s) 227, 247
SmiMI CAYNNNNRTG 2 cut(s) 566, 1149
SsiI CCGC 2 cut(s) 107, 165
SspI AATATT 2 cut(s) 351, 667
SspMI CTAG 1 cut(s) 506
SstI GAGCTC 1 cut(s) 1225
StyI CCWWGG 2 cut(s) 178, 444
TaaI ACNGT 2 cut(s) 64, 851
TaiI ACGT 1 cut(s) 1120
TaqI TCGA 1 cut(s) 42
TatI WGTACW 1 cut(s) 1251
TauI GCSGC 1 cut(s) 110
TfiI GAWTC 2 cut(s) 526, 1066
Tru1I TTAA 4 cut(s) 468, 552, 606, 1212
Tru9I TTAA 4 cut(s) 468, 552, 606, 1212
TscAI CASTG 3 cut(s) 153, 363, 1332
TseFI GTSAC 1 cut(s) 1142
TseI GCWGC 3 cut(s) 758, 1041, 1292
Tsp45I GTSAC 1 cut(s) 1142
TspDTI ATGAA 5 cut(s) 156, 324, 578, 660, 1162
TspGWI ACGGA 1 cut(s) 840
TspRI CASTG 3 cut(s) 153, 363, 1332
VpaK11BI GGWCC 2 cut(s) 227, 247
XagI CCTNNNNNAGG 1 cut(s) 182
XapI RAATTY 4 cut(s) 518, 767, 802, 1313
XcmI CCANNNNNNNNNTGG 1 cut(s) 1214
XspI CTAG 1 cut(s) 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.