RchiOBHm_Chr1g0375871

alpha-L-fucosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0003070
Physical Location & Seq
Reverse (-)
63182372 .. 63184226
1855 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59955

Sequence Viewer

Length: 1311 bp
ATGTTCCTCCATTTTGGAGTGAACACATTCACTGGCCTTGAGCGTGGCACCGGGCATGAAAACCCAGCCATATTCAACCCAGTTGCGCTTGATGCGAGCCAATGGGTGAGTGTAGCAGAAGATGCAGGCATATCACTAATGATACTTACTGCAAAGCACCATGATGGGTTTTGCTTGTGGCCTTCTAAGTACACTGACCATTCTGTGGAAAGAAGTCCTTGGAAAAATGGCCATGGCGATGTTGTTCAGGAGTTGGTTAATGCGGCTAAGCCCCGTGGGATTGACGTTGGGCTGTATCTCTCACCCTGGGACAGACATGATCGCAGATATGGGCTGGAGAAGCCATATAACGAATACTACTTGGCTCAGTTACAAGAGCTACTCAGTAGATATGGGAGTTTAAGAGAAATTTGGTTTGATGGATATAAAGGTGAACATGCGCCGAACATGTCCTATTATTTTACAGATTGGTTCTCTATGGTGAAGGAGTTGCAGAGTTCCATTAACATATTTTCTGATGCTGGTCCCGATGTTAGATGGGTTGGAAATGAGGCTGGGTTTGCGGGGAAAACATGCTGGTCCACCATCAATCGGACCTCGTTATCAATTGGGAATGCAAGCATTGAGGATTATCTCAACAAAGGTGATCCAAAAGGGACGGATTGGTTGCCAGCAGAGTGCGATGTCTCAATCAGAAAAGGATGGTTTTGGCATAGTTCAGAGTCACCTAAGAAGCTAAGCCAGCTACTCGAAATTTACTATAACTCAGTAGGACGAAATGCTGTGTTACTACTCAACGTACCTCCTAATTTGACAGGGCTCATATCCGAAGTAGATGTCCAAAGACTCCGAGAATTCAGAATTGCAATCGACACCATATTTTCTACCAATGTAGCAAAGAAATGCTCCATCAAGGTGAGTAGCCAAAGAGGGGGTACAGGAGGAGGTTTTGGACCTGAGAATGTGCTAGACAGTGACCATTTGTGGACATATTGGGCTCCAAGGGATGGTGACAAAGAGGACCACTGGATTGAATTCAGAGCAGATGAAGGGATGAGGTTCAATGTGATCAGGATTCAAGAGGCGATTGGGCTAGGTCAGAGGATCAAACGGCACGAAATTTGTGTCGATGGTAAAATAGTAGTTAATGGAACCACAGTGGGTCACAAGAGACTTCACAGACTTGAAGAGGGAGTAGTCCATGGCCAAACAGTAAGAATTAGAATTAAAGAATCAAGAGGAGGGCTTCCTTTAATTTCATCCATTGGCCTGCATTTTGATCCCTATTGGCACCCAGATAAAGGTGTGTGA

Protein Analysis

436

Amino Acids

49.29

Weight (kDa)

6.39

Isoelectric Point (pI)

36.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alpha_L_fucos PF01120 24 - 285 1.9e-34 Alpha-L-fucosidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 47, 1290
AccB7I CCANNNNNTGG 2 cut(s) 205, 1007
AciI CCGC 2 cut(s) 263, 563
AclWI GGATC 3 cut(s) 641, 1112, 1274
AcoI YGGCCR 2 cut(s) 229, 1204
AcsI RAATTY 5 cut(s) 408, 753, 854, 1034, 1119
AfaI GTAC 3 cut(s) 191, 801, 937
AfiI CCNNNNNNNGG 5 cut(s) 205, 591, 931, 1007, 1301
AflIII ACRYGT 1 cut(s) 447
AgsI TTSAA 5 cut(s) 76, 1034, 1063, 1079, 1187
AjnI CCWGG 1 cut(s) 305
AluBI AGCT 3 cut(s) 379, 736, 745
AluI AGCT 3 cut(s) 379, 736, 745
Alw26I GTCTC 2 cut(s) 691, 1165
AlwI GGATC 3 cut(s) 641, 1112, 1274
AoxI GGCC 5 cut(s) 34, 179, 229, 1204, 1267
ApoI RAATTY 5 cut(s) 408, 753, 854, 1034, 1119
ArsI GACNNNNNNTTYG 4 cut(s) 822, 854, 1110, 1142
Asp700I GAANNNNTTC 1 cut(s) 26
AspLEI GCGC 2 cut(s) 88, 442
AspS9I GGNCC 5 cut(s) 524, 579, 594, 953, 1021
AsuC2I CCSGG 1 cut(s) 52
AsuHPI GGTGA 8 cut(s) 118, 294, 443, 493, 656, 717, 928, 1022
AvaII GGWCC 5 cut(s) 524, 579, 594, 953, 1021
BalI TGGCCA 2 cut(s) 231, 1206
BanI GGYRCC 2 cut(s) 47, 1290
BanII GRGCYC 2 cut(s) 822, 1000
BccI CCATC 8 cut(s) 158, 413, 531, 593, 696, 917, 1001, 1124
BceAI ACGGC 1 cut(s) 1127
BciT130I CCWGG 1 cut(s) 307
BclI TGATCA 1 cut(s) 1068
BcnI CCSGG 1 cut(s) 52
BcoDI GTCTC 2 cut(s) 691, 1165
BfaI CTAG 2 cut(s) 968, 1094
BisI GCNGC 1 cut(s) 264
BlpI GCTNAGC 2 cut(s) 267, 737
BlsI GCNGC 1 cut(s) 265
Bme1390I CCNGG 2 cut(s) 52, 307
Bme18I GGWCC 5 cut(s) 524, 579, 594, 953, 1021
BmgT120I GGNCC 5 cut(s) 524, 579, 594, 953, 1021
BmiI GGNNCC 5 cut(s) 49, 526, 999, 1153, 1292
BmrFI CCNGG 2 cut(s) 52, 307
BmrI ACTGGG 1 cut(s) 74
BmsI GCATC 3 cut(s) 82, 112, 508
BmuI ACTGGG 1 cut(s) 74
BpmI CTGGAG 1 cut(s) 356
Bpu1102I GCTNAGC 2 cut(s) 267, 737
BpuEI CTTGAG 1 cut(s) 59
BpuMI CCSGG 1 cut(s) 52
BsaJI CCNNGG 7 cut(s) 218, 232, 274, 305, 306, 1001, 1201
Bsc4I CCNNNNNNNGG 5 cut(s) 205, 591, 931, 1007, 1301
Bse1I ACTGG 3 cut(s) 37, 80, 1031
BseBI CCWGG 1 cut(s) 307
BseDI CCNNGG 7 cut(s) 218, 232, 274, 305, 306, 1001, 1201
BseGI GGATG 4 cut(s) 707, 1012, 1059, 1259
BseLI CCNNNNNNNGG 5 cut(s) 205, 591, 931, 1007, 1301
BseMII CTCAG 4 cut(s) 380, 397, 780, 948
BseNI ACTGG 3 cut(s) 37, 80, 1031
BseRI GAGGAG 2 cut(s) 957, 1254
BseYI CCCAGC 2 cut(s) 64, 554
BshFI GGCC 5 cut(s) 36, 181, 231, 1206, 1269
BshNI GGYRCC 2 cut(s) 47, 1290
BsiSI CCGG 1 cut(s) 51
BslFI GGGAC 3 cut(s) 323, 510, 670
BslI CCNNNNNNNGG 5 cut(s) 205, 591, 931, 1007, 1301
BsmAI GTCTC 2 cut(s) 691, 1165
BsmFI GGGAC 3 cut(s) 323, 510, 670
BsmI GAATGC 1 cut(s) 619
BsnI GGCC 5 cut(s) 36, 181, 231, 1206, 1269
Bsp1286I GDGCHC 2 cut(s) 822, 1000
Bsp143I GATC 5 cut(s) 319, 646, 1068, 1104, 1279
Bsp1720I GCTNAGC 2 cut(s) 267, 737
Bsp19I CCATGG 2 cut(s) 232, 1201
BspACI CCGC 2 cut(s) 263, 563
BspANI GGCC 5 cut(s) 36, 181, 231, 1206, 1269
BspCNI CTCAG 4 cut(s) 379, 396, 779, 949
BspLI GGNNCC 5 cut(s) 49, 526, 999, 1153, 1292
BspPI GGATC 3 cut(s) 641, 1112, 1274
BspT107I GGYRCC 2 cut(s) 47, 1290
BsrI ACTGG 3 cut(s) 37, 80, 1031
BssECI CCNNGG 7 cut(s) 218, 232, 274, 305, 306, 1001, 1201
BssMI GATC 5 cut(s) 319, 646, 1068, 1104, 1279
BssT1I CCWWGG 4 cut(s) 218, 232, 1001, 1201
Bst2UI CCWGG 1 cut(s) 307
Bst4CI ACNGT 3 cut(s) 974, 1159, 1213
Bst6I CTCTTC 1 cut(s) 1182
BstAPI GCANNNNNTGC 1 cut(s) 122
BstC8I GCNNGC 6 cut(s) 97, 127, 619, 672, 743, 1271
BstDEI CTNAG 8 cut(s) 186, 267, 366, 383, 729, 737, 766, 957
BstDSI CCRYGG 3 cut(s) 232, 274, 1201
BstF5I GGATG 4 cut(s) 707, 1012, 1059, 1259
BstHHI GCGC 2 cut(s) 88, 442
BstKTI GATC 5 cut(s) 322, 649, 1071, 1107, 1282
BstMAI GTCTC 2 cut(s) 691, 1165
BstMBI GATC 5 cut(s) 319, 646, 1068, 1104, 1279
BstMWI GCNNNNNNNGC 5 cut(s) 92, 122, 340, 560, 742
BstNI CCWGG 1 cut(s) 307
BstNSI RCATGY 3 cut(s) 440, 451, 576
BstSCI CCNGG 2 cut(s) 50, 305
BsuRI GGCC 5 cut(s) 36, 181, 231, 1206, 1269
BtgI CCRYGG 3 cut(s) 232, 274, 1201
BtgZI GCGATG 2 cut(s) 252, 696
BtsCI GGATG 4 cut(s) 707, 1012, 1059, 1259
BtsIMutI CAGTG 5 cut(s) 30, 192, 979, 1024, 1164
Cac8I GCNNGC 6 cut(s) 97, 127, 619, 672, 743, 1271
CfoI GCGC 2 cut(s) 88, 442
Cfr13I GGNCC 5 cut(s) 524, 579, 594, 953, 1021
Csp6I GTAC 3 cut(s) 190, 800, 936
CviAII CATG 8 cut(s) 56, 161, 233, 317, 437, 448, 573, 1202
CviQI GTAC 3 cut(s) 190, 800, 936
DdeI CTNAG 8 cut(s) 186, 267, 366, 383, 729, 737, 766, 957
DpnI GATC 5 cut(s) 321, 648, 1070, 1106, 1281
DpnII GATC 5 cut(s) 319, 646, 1068, 1104, 1279
EaeI YGGCCR 2 cut(s) 229, 1204
Eam1104I CTCTTC 1 cut(s) 1182
EarI CTCTTC 1 cut(s) 1182
Eco130I CCWWGG 4 cut(s) 218, 232, 1001, 1201
Eco24I GRGCYC 2 cut(s) 822, 1000
Eco47I GGWCC 5 cut(s) 524, 579, 594, 953, 1021
EcoRI GAATTC 2 cut(s) 854, 1034
EcoRII CCWGG 1 cut(s) 305
EcoT14I CCWWGG 4 cut(s) 218, 232, 1001, 1201
EcoT38I GRGCYC 2 cut(s) 822, 1000
ErhI CCWWGG 4 cut(s) 218, 232, 1001, 1201
FaeI CATG 8 cut(s) 59, 164, 236, 320, 440, 451, 576, 1205
FalI AAGNNNNNCTT 2 cut(s) 202, 234
FaqI GGGAC 3 cut(s) 323, 510, 670
FatI CATG 8 cut(s) 55, 160, 232, 316, 436, 447, 572, 1201
FauI CCCGC 1 cut(s) 556
FbaI TGATCA 1 cut(s) 1068
Fnu4HI GCNGC 1 cut(s) 264
FokI GGATG 4 cut(s) 714, 1019, 1066, 1246
FriOI GRGCYC 2 cut(s) 822, 1000
Fsp4HI GCNGC 1 cut(s) 264
FspBI CTAG 2 cut(s) 968, 1094
GlaI GCGC 2 cut(s) 87, 441
GluI GCNGC 1 cut(s) 264
GsaI CCCAGC 2 cut(s) 68, 558
GsuI CTGGAG 1 cut(s) 356
HaeIII GGCC 5 cut(s) 36, 181, 231, 1206, 1269
HapII CCGG 1 cut(s) 51
HhaI GCGC 2 cut(s) 88, 442
Hin1II CATG 8 cut(s) 59, 164, 236, 320, 440, 451, 576, 1205
Hin6I GCGC 2 cut(s) 86, 440
HinP1I GCGC 2 cut(s) 86, 440
HinfI GANTC 4 cut(s) 722, 846, 1075, 1232
HpaII CCGG 1 cut(s) 51
HphI GGTGA 8 cut(s) 118, 294, 443, 493, 656, 717, 928, 1022
Hpy166II GTNNAC 5 cut(s) 22, 192, 434, 582, 987
Hpy188I TCNGA 9 cut(s) 517, 594, 695, 721, 829, 851, 860, 1040, 1101
Hpy188III TCNNGA 5 cut(s) 248, 527, 1072, 1079, 1236
Hpy8I GTNNAC 5 cut(s) 22, 192, 434, 582, 987
HpyAV CCTTC 3 cut(s) 192, 478, 1043
HpyCH4III ACNGT 3 cut(s) 974, 1159, 1213
HpyCH4IV ACGT 2 cut(s) 285, 798
HpyCH4V TGCA 6 cut(s) 125, 152, 493, 617, 866, 1273
HpyF10VI GCNNNNNNNGC 5 cut(s) 92, 122, 340, 560, 742
HpyF3I CTNAG 8 cut(s) 186, 267, 366, 383, 729, 737, 766, 957
HpySE526I ACGT 2 cut(s) 285, 798
Hsp92II CATG 8 cut(s) 59, 164, 236, 320, 440, 451, 576, 1205
HspAI GCGC 2 cut(s) 86, 440
Ksp22I TGATCA 1 cut(s) 1068
Kzo9I GATC 5 cut(s) 319, 646, 1068, 1104, 1279
LmnI GCTCC 2 cut(s) 911, 1003
LweI GCATC 3 cut(s) 82, 112, 508
MaeI CTAG 2 cut(s) 968, 1094
MaeII ACGT 2 cut(s) 285, 798
MaeIII GTNAC 6 cut(s) 369, 723, 786, 974, 1010, 1163
MalI GATC 5 cut(s) 321, 648, 1070, 1106, 1281
MboI GATC 5 cut(s) 319, 646, 1068, 1104, 1279
MboII GAAGA 2 cut(s) 131, 1199
MfeI CAATTG 1 cut(s) 606
MhlI GDGCHC 2 cut(s) 822, 1000
MlsI TGGCCA 2 cut(s) 231, 1206
MluNI TGGCCA 2 cut(s) 231, 1206
MlyI GAGTC 2 cut(s) 731, 840
MmeI TCCRAC 1 cut(s) 523
Mox20I TGGCCA 2 cut(s) 231, 1206
MroXI GAANNNNTTC 1 cut(s) 26
MscI TGGCCA 2 cut(s) 231, 1206
MseI TTAA 6 cut(s) 258, 401, 504, 1146, 1227, 1253
MslI CAYNNNNRTG 3 cut(s) 162, 237, 914
Msp20I TGGCCA 2 cut(s) 231, 1206
MspI CCGG 1 cut(s) 51
MspR9I CCNGG 2 cut(s) 52, 307
MunI CAATTG 1 cut(s) 606
Mva1269I GAATGC 1 cut(s) 619
MvaI CCWGG 1 cut(s) 307
MwoI GCNNNNNNNGC 5 cut(s) 92, 122, 340, 560, 742
NciI CCSGG 1 cut(s) 52
NcoI CCATGG 2 cut(s) 232, 1201
NdeII GATC 5 cut(s) 319, 646, 1068, 1104, 1279
NlaIII CATG 8 cut(s) 59, 164, 236, 320, 440, 451, 576, 1205
NlaIV GGNNCC 5 cut(s) 49, 526, 999, 1153, 1292
NmuCI GTSAC 4 cut(s) 723, 974, 1010, 1163
NspI RCATGY 3 cut(s) 440, 451, 576
PasI CCCWGGG 1 cut(s) 306
PciI ACATGT 1 cut(s) 447
PctI GAATGC 1 cut(s) 619
PdmI GAANNNNTTC 1 cut(s) 26
PfeI GAWTC 2 cut(s) 1075, 1232
PflMI CCANNNNNTGG 2 cut(s) 205, 1007
PkrI GCNGC 1 cut(s) 265
PleI GAGTC 2 cut(s) 730, 840
PpsI GAGTC 2 cut(s) 730, 840
PscI ACATGT 1 cut(s) 447
Psp6I CCWGG 1 cut(s) 305
PspFI CCCAGC 2 cut(s) 64, 554
PspGI CCWGG 1 cut(s) 305
PspN4I GGNNCC 5 cut(s) 49, 526, 999, 1153, 1292
PspPI GGNCC 5 cut(s) 524, 579, 594, 953, 1021
RsaI GTAC 3 cut(s) 191, 801, 937
RsaNI GTAC 3 cut(s) 190, 800, 936
RseI CAYNNNNRTG 3 cut(s) 162, 237, 914
SaqAI TTAA 6 cut(s) 258, 401, 504, 1146, 1227, 1253
SatI GCNGC 1 cut(s) 264
Sau3AI GATC 5 cut(s) 319, 646, 1068, 1104, 1279
Sau96I GGNCC 5 cut(s) 524, 579, 594, 953, 1021
SchI GAGTC 2 cut(s) 731, 840
ScrFI CCNGG 2 cut(s) 52, 307
SduI GDGCHC 2 cut(s) 822, 1000
SfaNI GCATC 3 cut(s) 82, 112, 508
SinI GGWCC 5 cut(s) 524, 579, 594, 953, 1021
SmiMI CAYNNNNRTG 3 cut(s) 162, 237, 914
SmlI CTYRAG 1 cut(s) 38
SmoI CTYRAG 1 cut(s) 38
SsiI CCGC 2 cut(s) 263, 563
SspMI CTAG 2 cut(s) 968, 1094
StyD4I CCNGG 2 cut(s) 50, 305
StyI CCWWGG 4 cut(s) 218, 232, 1001, 1201
TaaI ACNGT 3 cut(s) 974, 1159, 1213
TaiI ACGT 2 cut(s) 288, 801
TaqI TCGA 3 cut(s) 750, 870, 1128
TatI WGTACW 1 cut(s) 189
TauI GCSGC 1 cut(s) 266
TfiI GAWTC 2 cut(s) 1075, 1232
Tru1I TTAA 6 cut(s) 258, 401, 504, 1146, 1227, 1253
Tru9I TTAA 6 cut(s) 258, 401, 504, 1146, 1227, 1253
TscAI CASTG 5 cut(s) 37, 199, 979, 1031, 1164
TseFI GTSAC 4 cut(s) 723, 974, 1010, 1163
Tsp45I GTSAC 4 cut(s) 723, 974, 1010, 1163
TspDTI ATGAA 3 cut(s) 72, 1062, 1248
TspGWI ACGGA 1 cut(s) 674
TspRI CASTG 5 cut(s) 37, 199, 979, 1031, 1164
Van91I CCANNNNNTGG 2 cut(s) 205, 1007
VpaK11BI GGWCC 5 cut(s) 524, 579, 594, 953, 1021
XapI RAATTY 5 cut(s) 408, 753, 854, 1034, 1119
XceI RCATGY 3 cut(s) 440, 451, 576
XmnI GAANNNNTTC 1 cut(s) 26
XspI CTAG 2 cut(s) 968, 1094
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.