RLG00000026648

alpha-L-fucosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0003070
Physical Location & Seq
Forward (+)
4583715 .. 4585215
1501 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026648

Sequence Viewer

Length: 1209 bp
ATGGGTAAACAATGGTGCCCATTGTTCATAATCACATTCTTTGAACTATGCACATTTACATTCTCTAGCCTTGAGCAAGTAACAACACCACCCTTGCCAGTTCTACCACTTCCTACGTATCAACAACTGAAATGGCAACAAAGGGAGATAATAATGTTCCTCCATTTTGGAGTGAACACATTCACTGGCCTTGAGCGTGGCACTGGGCATGAAAACCCAGCCATATTCAACCCAGTTGCGCTTGACGCGAGCCAATGGGTGAGTGTAGCAGAAGATGCAGGCATATCACTAATGATACTTACTGCAAAGCACTATGATGGGTTTTGCTTGTGGCCTTCTAAGTACACTGACCATTCTGTGGAAAGAAGTCCTTGGAAAAATGGCCATGGCGATGTTGTTCAGGAGTTGGTTAATGCGGCTAAGCCCCGTGGGATTGACGTTGGGCTGTATCTCTCACCCTGGGACAGACATGATCGCAGATATGGGCTGGAGAAGCCATATAACGAATACTACTTGGCTCAGTTACAAGAGCTACTCAGTAGATACGGGAGTTTAAGAGAAATTTGGTTTGATGGATATAAAGGTGAACATGCGCCAAACATGTCCTATTATTTTACAGATTGGTTCTCTATGGTGGAAGAGTTGCAGAGTTCCATTAACATATTTTCTGATGCTGGTCCTGATGTTAGATGGGTTGGAAATGAGGCTGGGTTTGCAGGGAAAACATGCTGGTCCACCATCAATCGGACCTCGTTATCAATTGGGAATGCAAGCATTGAGGATTATCTCAACAAAGGTGATCCAAAAGGGACAGATTGGTTGCCAGCGGAGTGCGATGTCTCAATCAGAAAAGGATGGTTTTGGCATAGTTCAGAGTCACCTAAGAAGCTAAGCCAGCTACTCGAAATTTACTATAACTCAGTAGGACGAAATGCTGTGTTACTACTCAACGTGCCTCCTAATTCGACAGGGCTCATATCTGAAGTAGATGTCCACAGACTCCGAGAATTCAGAATTGCAATCGACACCATTTTTTCGACCAATGTAGCAGAGAAATGCTCCATCAAGGTGAGTAGCCAAAGAGGGGGTACGGGAGGAGGTTTGGACCTGAGAATGTGCTCGACAGTGACCATTTGCGGACATATTGGGCTCCAAGGGATGGTGACAAAGAGGACCACTGGATTGAATTCAGAGCAGATGAAGGGATGA

Protein Analysis

403

Amino Acids

45.31

Weight (kDa)

5.96

Isoelectric Point (pI)

46.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alpha_L_fucos PF01120 74 - 336 2e-33 Alpha-L-fucosidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 15
AccB7I CCANNNNNTGG 2 cut(s) 358, 1159
AccII CGCG 1 cut(s) 248
AciI CCGC 3 cut(s) 416, 827, 1137
AclWI GGATC 1 cut(s) 794
AcoI YGGCCR 1 cut(s) 382
AcsI RAATTY 4 cut(s) 561, 906, 1007, 1186
AcuI CTGAAG 1 cut(s) 1002
AfaI GTAC 2 cut(s) 344, 1090
AfiI CCNNNNNNNGG 4 cut(s) 358, 744, 1084, 1159
AflIII ACRYGT 1 cut(s) 600
AgsI TTSAA 3 cut(s) 44, 229, 1186
AjnI CCWGG 1 cut(s) 458
AluBI AGCT 3 cut(s) 532, 889, 898
AluI AGCT 3 cut(s) 532, 889, 898
Alw21I GWGCWC 1 cut(s) 1121
Alw26I GTCTC 1 cut(s) 844
AlwI GGATC 1 cut(s) 794
AoxI GGCC 3 cut(s) 187, 332, 382
ApoI RAATTY 4 cut(s) 561, 906, 1007, 1186
Asp700I GAANNNNTTC 1 cut(s) 179
AspLEI GCGC 2 cut(s) 241, 595
AspS9I GGNCC 5 cut(s) 677, 732, 747, 1105, 1173
AsuHPI GGTGA 7 cut(s) 271, 447, 596, 809, 870, 1081, 1174
AvaII GGWCC 5 cut(s) 677, 732, 747, 1105, 1173
BaeGI GKGCMC 1 cut(s) 20
BaeI ACNNNNGTAYC 2 cut(s) 535, 568
BalI TGGCCA 1 cut(s) 384
BanI GGYRCC 1 cut(s) 15
BanII GRGCYC 2 cut(s) 975, 1152
Bbv12I GWGCWC 1 cut(s) 1121
BccI CCATC 7 cut(s) 311, 566, 684, 746, 849, 1070, 1153
BciT130I CCWGG 1 cut(s) 460
BcoDI GTCTC 1 cut(s) 844
BfaI CTAG 1 cut(s) 66
BisI GCNGC 1 cut(s) 417
BlpI GCTNAGC 2 cut(s) 420, 890
BlsI GCNGC 1 cut(s) 418
Bme1390I CCNGG 1 cut(s) 460
Bme18I GGWCC 5 cut(s) 677, 732, 747, 1105, 1173
BmgT120I GGNCC 5 cut(s) 677, 732, 747, 1105, 1173
BmiI GGNNCC 2 cut(s) 17, 1151
BmrFI CCNGG 1 cut(s) 460
BmrI ACTGGG 2 cut(s) 213, 227
BmsI GCATC 2 cut(s) 265, 661
BmuI ACTGGG 2 cut(s) 213, 227
BplI GAGNNNNNCTC 2 cut(s) 1043, 1075
BpmI CTGGAG 1 cut(s) 509
Bpu1102I GCTNAGC 2 cut(s) 420, 890
BpuEI CTTGAG 2 cut(s) 92, 212
BsaAI YACGTR 1 cut(s) 117
BsaJI CCNNGG 6 cut(s) 371, 385, 427, 458, 459, 1153
Bsc4I CCNNNNNNNGG 4 cut(s) 358, 744, 1084, 1159
Bse1I ACTGG 5 cut(s) 98, 190, 208, 233, 1183
BseBI CCWGG 1 cut(s) 460
BseDI CCNNGG 6 cut(s) 371, 385, 427, 458, 459, 1153
BseGI GGATG 2 cut(s) 860, 1164
BseLI CCNNNNNNNGG 4 cut(s) 358, 744, 1084, 1159
BseMII CTCAG 4 cut(s) 533, 550, 933, 1100
BseNI ACTGG 5 cut(s) 98, 190, 208, 233, 1183
BseRI GAGGAG 1 cut(s) 1110
BseSI GKGCMC 1 cut(s) 20
BseYI CCCAGC 2 cut(s) 217, 707
Bsh1236I CGCG 1 cut(s) 248
BshFI GGCC 3 cut(s) 189, 334, 384
BshNI GGYRCC 1 cut(s) 15
BsiHKAI GWGCWC 1 cut(s) 1121
BslFI GGGAC 2 cut(s) 476, 823
BslI CCNNNNNNNGG 4 cut(s) 358, 744, 1084, 1159
BsmAI GTCTC 1 cut(s) 844
BsmFI GGGAC 2 cut(s) 476, 823
BsmI GAATGC 1 cut(s) 772
BsnI GGCC 3 cut(s) 189, 334, 384
Bsp1286I GDGCHC 4 cut(s) 20, 975, 1121, 1152
Bsp143I GATC 2 cut(s) 472, 799
Bsp1720I GCTNAGC 2 cut(s) 420, 890
Bsp19I CCATGG 1 cut(s) 385
BspACI CCGC 3 cut(s) 416, 827, 1137
BspANI GGCC 3 cut(s) 189, 334, 384
BspCNI CTCAG 4 cut(s) 532, 549, 932, 1101
BspFNI CGCG 1 cut(s) 248
BspLI GGNNCC 2 cut(s) 17, 1151
BspPI GGATC 1 cut(s) 794
BspT107I GGYRCC 1 cut(s) 15
BsrI ACTGG 5 cut(s) 98, 190, 208, 233, 1183
BssECI CCNNGG 6 cut(s) 371, 385, 427, 458, 459, 1153
BssMI GATC 2 cut(s) 472, 799
BssT1I CCWWGG 3 cut(s) 371, 385, 1153
Bst2UI CCWGG 1 cut(s) 460
Bst4CI ACNGT 1 cut(s) 1126
Bst6I CTCTTC 1 cut(s) 633
BstAPI GCANNNNNTGC 1 cut(s) 275
BstBAI YACGTR 1 cut(s) 117
BstC8I GCNNGC 5 cut(s) 250, 280, 772, 825, 896
BstDEI CTNAG 8 cut(s) 339, 420, 519, 536, 882, 890, 919, 1109
BstDSI CCRYGG 2 cut(s) 385, 427
BstF5I GGATG 2 cut(s) 860, 1164
BstFNI CGCG 1 cut(s) 248
BstHHI GCGC 2 cut(s) 241, 595
BstKTI GATC 2 cut(s) 475, 802
BstMAI GTCTC 1 cut(s) 844
BstMBI GATC 2 cut(s) 472, 799
BstMWI GCNNNNNNNGC 5 cut(s) 245, 275, 493, 713, 895
BstNI CCWGG 1 cut(s) 460
BstNSI RCATGY 3 cut(s) 593, 604, 729
BstSCI CCNGG 1 cut(s) 458
BstSLI GKGCMC 1 cut(s) 20
BstSNI TACGTA 1 cut(s) 117
BstUI CGCG 1 cut(s) 248
BsuRI GGCC 3 cut(s) 189, 334, 384
BtgI CCRYGG 2 cut(s) 385, 427
BtgZI GCGATG 2 cut(s) 405, 849
BtsCI GGATG 2 cut(s) 860, 1164
BtsIMutI CAGTG 5 cut(s) 183, 201, 345, 1131, 1176
Cac8I GCNNGC 5 cut(s) 250, 280, 772, 825, 896
CfoI GCGC 2 cut(s) 241, 595
Cfr13I GGNCC 5 cut(s) 677, 732, 747, 1105, 1173
CseI GACGC 1 cut(s) 254
Csp6I GTAC 2 cut(s) 343, 1089
CviAII CATG 6 cut(s) 209, 386, 470, 590, 601, 726
CviQI GTAC 2 cut(s) 343, 1089
DdeI CTNAG 8 cut(s) 339, 420, 519, 536, 882, 890, 919, 1109
DpnI GATC 2 cut(s) 474, 801
DpnII GATC 2 cut(s) 472, 799
EaeI YGGCCR 1 cut(s) 382
Eam1104I CTCTTC 1 cut(s) 633
EarI CTCTTC 1 cut(s) 633
Eco105I TACGTA 1 cut(s) 117
Eco130I CCWWGG 3 cut(s) 371, 385, 1153
Eco24I GRGCYC 2 cut(s) 975, 1152
Eco47I GGWCC 5 cut(s) 677, 732, 747, 1105, 1173
Eco57I CTGAAG 1 cut(s) 1002
EcoRI GAATTC 2 cut(s) 1007, 1186
EcoRII CCWGG 1 cut(s) 458
EcoT14I CCWWGG 3 cut(s) 371, 385, 1153
EcoT38I GRGCYC 2 cut(s) 975, 1152
ErhI CCWWGG 3 cut(s) 371, 385, 1153
FaeI CATG 6 cut(s) 212, 389, 473, 593, 604, 729
FalI AAGNNNNNCTT 2 cut(s) 355, 387
FaqI GGGAC 2 cut(s) 476, 823
FatI CATG 6 cut(s) 208, 385, 469, 589, 600, 725
Fnu4HI GCNGC 1 cut(s) 417
FokI GGATG 2 cut(s) 867, 1171
FriOI GRGCYC 2 cut(s) 975, 1152
Fsp4HI GCNGC 1 cut(s) 417
FspBI CTAG 1 cut(s) 66
GlaI GCGC 2 cut(s) 240, 594
GluI GCNGC 1 cut(s) 417
GsaI CCCAGC 2 cut(s) 221, 711
GsuI CTGGAG 1 cut(s) 509
HaeIII GGCC 3 cut(s) 189, 334, 384
HgaI GACGC 1 cut(s) 254
HhaI GCGC 2 cut(s) 241, 595
Hin1II CATG 6 cut(s) 212, 389, 473, 593, 604, 729
Hin6I GCGC 2 cut(s) 239, 593
HinP1I GCGC 2 cut(s) 239, 593
HinfI GANTC 2 cut(s) 875, 999
HphI GGTGA 7 cut(s) 271, 447, 596, 809, 870, 1081, 1174
Hpy166II GTNNAC 6 cut(s) 8, 175, 345, 587, 735, 994
Hpy188I TCNGA 8 cut(s) 670, 747, 848, 874, 982, 1004, 1013, 1192
Hpy188III TCNNGA 2 cut(s) 401, 680
Hpy8I GTNNAC 6 cut(s) 8, 175, 345, 587, 735, 994
HpyAV CCTTC 2 cut(s) 345, 1195
HpyCH4III ACNGT 1 cut(s) 1126
HpyCH4IV ACGT 3 cut(s) 116, 438, 951
HpyCH4V TGCA 7 cut(s) 51, 278, 305, 646, 716, 770, 1019
HpyF10VI GCNNNNNNNGC 5 cut(s) 245, 275, 493, 713, 895
HpyF3I CTNAG 8 cut(s) 339, 420, 519, 536, 882, 890, 919, 1109
HpySE526I ACGT 3 cut(s) 116, 438, 951
Hsp92II CATG 6 cut(s) 212, 389, 473, 593, 604, 729
HspAI GCGC 2 cut(s) 239, 593
Kzo9I GATC 2 cut(s) 472, 799
LmnI GCTCC 2 cut(s) 1064, 1155
LweI GCATC 2 cut(s) 265, 661
MaeI CTAG 1 cut(s) 66
MaeII ACGT 3 cut(s) 116, 438, 951
MaeIII GTNAC 6 cut(s) 79, 522, 876, 939, 1126, 1162
MalI GATC 2 cut(s) 474, 801
MboI GATC 2 cut(s) 472, 799
MboII GAAGA 2 cut(s) 284, 650
MfeI CAATTG 1 cut(s) 759
MhlI GDGCHC 4 cut(s) 20, 975, 1121, 1152
MlsI TGGCCA 1 cut(s) 384
MluCI AATT 7 cut(s) 561, 759, 906, 961, 1007, 1014, 1186
MluNI TGGCCA 1 cut(s) 384
MlyI GAGTC 2 cut(s) 884, 993
MmeI TCCRAC 1 cut(s) 676
MnlI CCTC 9 cut(s) 170, 697, 760, 772, 966, 1076, 1088, 1091, 1164
Mox20I TGGCCA 1 cut(s) 384
MroXI GAANNNNTTC 1 cut(s) 179
MscI TGGCCA 1 cut(s) 384
MseI TTAA 3 cut(s) 411, 554, 657
MslI CAYNNNNRTG 3 cut(s) 315, 390, 1067
Msp20I TGGCCA 1 cut(s) 384
MspA1I CMGCKG 1 cut(s) 827
MspR9I CCNGG 1 cut(s) 460
MunI CAATTG 1 cut(s) 759
Mva1269I GAATGC 1 cut(s) 772
MvaI CCWGG 1 cut(s) 460
MvnI CGCG 1 cut(s) 248
MwoI GCNNNNNNNGC 5 cut(s) 245, 275, 493, 713, 895
NcoI CCATGG 1 cut(s) 385
NdeII GATC 2 cut(s) 472, 799
NlaIII CATG 6 cut(s) 212, 389, 473, 593, 604, 729
NlaIV GGNNCC 2 cut(s) 17, 1151
NmuCI GTSAC 3 cut(s) 876, 1126, 1162
NspI RCATGY 3 cut(s) 593, 604, 729
PasI CCCWGGG 1 cut(s) 459
PciI ACATGT 1 cut(s) 600
PctI GAATGC 1 cut(s) 772
PdmI GAANNNNTTC 1 cut(s) 179
PflMI CCANNNNNTGG 2 cut(s) 358, 1159
PkrI GCNGC 1 cut(s) 418
PleI GAGTC 2 cut(s) 883, 993
PpsI GAGTC 2 cut(s) 883, 993
Ppu21I YACGTR 1 cut(s) 117
PscI ACATGT 1 cut(s) 600
Psp6I CCWGG 1 cut(s) 458
PspFI CCCAGC 2 cut(s) 217, 707
PspGI CCWGG 1 cut(s) 458
PspN4I GGNNCC 2 cut(s) 17, 1151
PspPI GGNCC 5 cut(s) 677, 732, 747, 1105, 1173
RsaI GTAC 2 cut(s) 344, 1090
RsaNI GTAC 2 cut(s) 343, 1089
RseI CAYNNNNRTG 3 cut(s) 315, 390, 1067
SaqAI TTAA 3 cut(s) 411, 554, 657
SatI GCNGC 1 cut(s) 417
Sau3AI GATC 2 cut(s) 472, 799
Sau96I GGNCC 5 cut(s) 677, 732, 747, 1105, 1173
SchI GAGTC 2 cut(s) 884, 993
ScrFI CCNGG 1 cut(s) 460
SduI GDGCHC 4 cut(s) 20, 975, 1121, 1152
SfaNI GCATC 2 cut(s) 265, 661
SinI GGWCC 5 cut(s) 677, 732, 747, 1105, 1173
SmiMI CAYNNNNRTG 3 cut(s) 315, 390, 1067
SmlI CTYRAG 2 cut(s) 71, 191
SmoI CTYRAG 2 cut(s) 71, 191
SnaBI TACGTA 1 cut(s) 117
Sse9I AATT 7 cut(s) 561, 759, 906, 961, 1007, 1014, 1186
SsiI CCGC 3 cut(s) 416, 827, 1137
SspMI CTAG 1 cut(s) 66
StyD4I CCNGG 1 cut(s) 458
StyI CCWWGG 3 cut(s) 371, 385, 1153
TaaI ACNGT 1 cut(s) 1126
TaiI ACGT 3 cut(s) 119, 441, 954
TaqI TCGA 5 cut(s) 903, 965, 1023, 1037, 1121
TasI AATT 7 cut(s) 561, 759, 906, 961, 1007, 1014, 1186
TatI WGTACW 1 cut(s) 342
TauI GCSGC 1 cut(s) 419
Tru1I TTAA 3 cut(s) 411, 554, 657
Tru9I TTAA 3 cut(s) 411, 554, 657
TscAI CASTG 5 cut(s) 190, 208, 352, 1131, 1183
TseFI GTSAC 3 cut(s) 876, 1126, 1162
Tsp45I GTSAC 3 cut(s) 876, 1126, 1162
TspDTI ATGAA 2 cut(s) 16, 225
TspRI CASTG 5 cut(s) 190, 208, 352, 1131, 1183
Van91I CCANNNNNTGG 2 cut(s) 358, 1159
VpaK11BI GGWCC 5 cut(s) 677, 732, 747, 1105, 1173
XapI RAATTY 4 cut(s) 561, 906, 1007, 1186
XceI RCATGY 3 cut(s) 593, 604, 729
XmnI GAANNNNTTC 1 cut(s) 179
XspI CTAG 1 cut(s) 66
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.